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The nucleotide sequence of a tRNA gene cluster from Spiroplasma meliferum.

FEATURES: The recombinant plasmid containing a tRNA gene cluster from Spiroplasma species BC3 has been described (1). The ten tRNA genes presumably constitute a single transcriptional unit. Probable promoter and terminators are indicated. A sequence GGGG (155-158) may serve as a 'Discriminator' region (2). An o.r.f. starts at nucleotide 1251 if UGA codes for tryptophan (3) . COMMENTS: The order of the tRNA genqs in this cluster, and the isoacceptors encoded (except for the first tRNAuYs gene), is identical to that of two other tRNA gene clusters from Gram-positive eubacteria; from Mvcoplasma mycoides (4), and a portion of the rrnB (trrnE) sequence from Bacillus subt-ilis (5,6). Similar clusters of tRNA genes implies an important transcription- al and regulatory mechanism for these gene clusters.