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Fractionation of Poly(γ-b-enzyl-L-glutamate) by Gel-Permeation Chromatography

Gel-permeation chromatography (GPC) was utilized for fractionating poly(γ-benzyl-L-glutamate) (PBLG) polymerized from N-carboxylanhydrides. The resolving power of this GPC column was checked by several monodisperse materials such as Gramicidin D, carbobenzoxy-L-Leu-L-Leu-L-Val-L-Phe-methyl ester, diketopiperazine, and 1,2,4-trichlorobenzene (TCB). The fractionation efficiency was analyzed by comparison of the molecular-weight distribution of fractionated PBLG and that of unfractionated PBLG, which was determined by the relation between molecular weights of PBLG samples and their elution volumes. On the basis of these results we conclude that GPC offers a useful method of obtaining the monodisperse PBLG whose Mw/Mn ratio is less than 1.1.