{'fraction_non_alphanumeric': 0.044617958728388175, 'fraction_numerical': 0.04210819854991634, 'mean_word_length': 5.079661016949153, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Genome Sequence of a Cluster DN1 Gordonia terrae Phage, Periwinkle

ABSTRACT Periwinkle is a temperate bacteriophage that was isolated on the host Gordonia terrae 3612. The genome has a length of 55,657 bp and a GC content of 62.9% and contains 109 protein-coding genes and no tRNA genes. An 8-kb region after the structural protein genes encodes eight membrane proteins, a tyrosine integrase, and an immunity repressor.

A ctinobacteriophages are extremely abundant and diverse viruses that infect bacteria within the phylum Actinobacteria (1-4). By studying bacteriophages through programs such as the Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) Program, we advance our understanding of phage and bacterial diversity, evolution, and virus-host interactions (3)(4)(5)(6). Periwinkle was isolated from a composted manure sample collected in Orono, Maine (44.915628N, 68.69072W), using Gordonia terrae 3612 (7). Soil extracts were prepared in peptone-yeast extract-calcium (PYCa) medium, filtered on 0.22-mm filters, inoculated with G. terrae, and incubated at 30°C for 48 h. Dilutions of the enriched extract were plated onto PYCa agar in soft agar containing G. terrae, and plaques were purified by five rounds of plaque assays (7). Periwinkle formed 3-mm turbid plaques on a lawn of G. terrae (7). Periwinkle has a Siphoviridae particle morphology, as determined by negative staining transmission electron microscopy. The particle has a 65-nm (standard error [SE], 60.8 nm) icosahedral head and a 335-nm (SE, 68.2 nm) flexible, noncontractile tail (n = 5).
A phenol-chloroform extraction method was used to extract DNA from a high-titer lysate before it was prepared for sequencing using the NEBNext Ultra II library preparation kit (New England BioLabs, Ipswich, MA) (8). Sequencing on an Illumina MiSeq platform yielded 168,288 single-end 150-bp reads. Newbler v2.9 and Consed v29 (9) were used for de novo assembly and checks for completeness, yielding a 55,657-bp genome with a GC content of 58.1%. Genome ends are defined by single-stranded 10-bp 39 extensions (CTCGGGGCAT). Periwinkle shares .35% gene content with members of cluster DN in the Phamerator Actino_Draft database and was assigned to subcluster DN1 (4, 10, 11).
The integrase and immunity repressor genes are located in an 8-kb region following the minor tail protein genes (gp35 and gp36) that contains forward-and reverse-transcribed genes (gp37 to gp58) that are likely expressed during lysogeny (19). Included are two DNA-binding proteins (gp42 and gp47), an acetyltransferase (gp37), and seven membrane proteins (gp40, gp41, gp44 to gp46, and gp48) that could contribute to superinfection immunity (19).
Data availability. Periwinkle is available in GenBank with the accession number ON456334 and the Sequence Read Archive (SRA) accession number SRR18715698.

ACKNOWLEDGMENTS
This research was made possible by the SEA-PHAGES program at the Howard Hughes Medical Institute. We thank Daniel Russel and Rebecca Garlena for sequencing services in assembling the Periwinkle genome. We are grateful to Geoff Williams at the Brown Bioimaging Facility for providing electron microscopy services. Students are grateful for the support of teaching assistants Remi Geohagen, Mathew Cox, Allie Conner, Caitlin Wiafe-Kwakye, Abigail McNally, and Jacob Cote.
Research reported in this project was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant P20GM103423.