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Simultaneous forward and backward digital holography of microparticles

This work explores several techniques in digital holography to image 10–300 μm sized particles and provide information useful for their morphological characterization. In particular, digital holograms are formed with both forward and backward-scattered light from samples fixed to a glass stage. Images of these particles are then rendered from the holograms demonstrating that front and back perspectives of a particle can be acquired simultaneously. In addition, holography’s large depth of field is used to investigate the potential for stand-off interrogation of particles of different types.