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Specific [3H]Piflutixol Binding to CHAPS‐Solubilised Rat Striatal Preparations Involves Dopamine D‐2 but Not D‐1 Binding Sites

Abstract: [3H]Piflutixol binding to rat striatal membrane preparations identifies both D‐l and D‐2 sites. We used [3H]piflutixol to characterise those binding sites present in 3‐[(3‐cholamidopropyl)dimethylammonio]‐1‐propanesulfonate (CHAPS)‐solubilised rat striatal preparations. The specific binding of [3H]piflutixol, as defined using cis‐flupenthixol, to CHAPS‐solubilised rat striatal tissue was saturable and of high affinity. Specific [3H]piflutixol binding to the solubilised preparations was displaced stereoselectively by the isomers of butaclamol and to an equal extent by both cis‐flupenthixol and (±)‐sulpiride. A positive correlation was found between the capacity of a range of drugs to displace [3H]piflutixol binding and the displacement of [3H]spiperone to the same preparations. The Bmax of [3H]piflutixol binding was not different from that of [3H]spiperone binding to the same preparation. These studies suggest that, in contrast to specific binding of membrane preparations, the specific binding of [3H]piflutixol to CHAPS‐solubilised preparations involves mainly D‐2 sites. Specific [3H]piflutixol binding, in contrast to [3H]spiperone binding, showed only slow dissociation from soluble preparations. The binding of [3H]piflutixol to CHAPS‐solubilised preparations was retained during passage through a gel filtration column. This prelabelling of solubilised striatal preparations using [3H]piflutixol may aid in the purification of CHAPS‐solubilised rat striatal D‐2 sites.