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Checklist of the continental fishes of the state of Chiapas, Mexico, and their distribution

Abstract An updated checklist of the distribution of fishes that inhabit the continental waters of the Mexican state of Chiapas is presented. The state was compartmentalized into 12 hydrological regions for the purpose of understanding the distribution of fish fauna across a state with large physiographic variance. The ichthyofauna of Chiapas is represented by 311 species distributed in two classes, 26 orders, 73 families, and 182 genera, including 12 exotic species. The families with the highest number of species were Cichlidae, Poeciliidae, Sciaenidae, Carangidae, Ariidae, Gobiidae, and Haemulidae. This study attempts to close gaps in knowledge of the distribution of ichthyofauna in the diverse hydrological regions of Chiapas, Mexico.


Introduction
The hydrological wealth of Chiapas is manifested through its 72 perennial rivers and abundant streams, lakes, and ponds. The presence of large hydroelectric dams has significantly increased the surface area of the state's bodies of water (Velasco-Colín 1976). Chiapas has a coastline of 270 km and more than 70,000 hectares of estuaries and coastal lagoons (Contreras-Espinosa 2010), which favors the presence of rich fish diversity (Velasco-Colín 1976, Lozano-Vilano and Contreras-Balderas 1987. Much of the state is located in the Usumacinta ichthyographic province/area of endemism (Miller et al. 2005, which means that its continental waters host a high number of endemic species, making Chiapas a freshwater biodiversity hotspot (Hudson et al. 2005. Several attempts have been made to record continental water fish diversity in Chiapas through numerous works such as checklists, annotated checklists, books and scattered records in the literature (e.g. Velasco-Colín 1976, Lozano-Vilano and Contreras-Balderas 1987, Lazcano-Barrero and Vogt 1992, Tapia-García et al. 1998, Rodiles-Hernández et al. 1999, Rodiles-Hernández 2005, Lozano-Vilano et al. 2007, González-Díaz et al. 2008, Espinosa-Pérez et al. 2011, Velázquez-Velázquez et al. 2013, Gómez-González et al. 2012. The first comprehensive publication on continental fishes of Chiapas was made by Velasco-Colín (1976), who reported 74 species distributed across 28 families. He also included brief information about the ecology, biology and distribution of several species and, in some cases, added relevant fishing information.
Subsequently Lozano-Vilano and Contreras-Balderas (1987) published an annotated checklist in which they registered 135 species belonging to 38 families in the state's continental waters. In addition to an increase in the number of data records, for the first time the distribution of fishes was associated with seven of the state's physiographic regions. Eighteen years later Rodiles-Hernández (2005) and Rodiles-  recorded 205 species in 44 families and 207 species in 45 families respectively. In the first study, distributions were reported at the level of the two main Chiapas river basins, the Grijalva-Usumacinta and the Coast of Chiapas, whereas, in the second study, the distributional geographic units were the Atlantic and the Pacific slope. Velázquez-Velázquez et al. (2013) was the last published attempt to summarize continental fishes of Chiapas. They reported 262 species across 57 families, and once again the geographic distribution units were the Grijalva and the Usumacinta River basins and the coast of Chiapas.
Two interesting trends emerge about the continental fishes of Chiapas. First, the number of recorded species has continued to increase over time likely due to an increase in sampling localities, implementation of new sampling techniques, new records and species descriptions. The second trend is related to the geographic units in which the state has been divided. For instance, Lozano-Vilano and Contreras-Balderas (1987) divided the state into seven physiographic regions, based on terrestrial relief. Most studies used broad delineations limited to the three major hydrologic regions (coast of Chiapas and the Grijalva and Usumacinta River basins) masking detailed information on finer distributional patterns like localized endemism and drainage interconnections.
Therefore, the aim of this study is to provide an updated checklist of the continental fishes of Chiapas, including distribution data, based on extensive literature research and complemented with material deposited in the ichthyological collection of the Museum of Zoology at the University of Arts and Sciences of Chiapas (MZ-P-UNICACH). For the first time, we use finer scale geographic divisions for the state, implemented at the sub-basin level, following the National Institute of Statistics and Geography (INEGI 2010).
Species were systematically arranged by order and family following Nelson (2006). Genera and species were arranged alphabetically; scientific names and authorities were corroborated following Eschmeyer et al. (2016). Tolerance to salinity was based on Myers (1938).
The 12 geographical units for Chiapas ( Figure 1) were utilized to determine the distribution of each species across the state. These 12 units were based on existing hydrological sub-basins of the state (INEGI 2010). The main rivers, ponds, lakes and coastal lagoons of each sub-basin are listed in Table 1.

Results
The continental fishes of the state of Chiapas are represented by two classes, 26 orders, 73 families, 182 genera and 311 species (    tolerance, and excluding exotic species, 16 are primary freshwater fishes, 65 secondary freshwater fishes, and the rest of the species are peripheral (Table 2). Of the 12 geographical units (Fig. 1), the region with the highest number of species was Costa-Itsmo with 174 species, followed by Costa-Soconusco with 153 species and the third was Usumacinta-Catazajá with 72 species. The region with the lowest recorded species was Usumacinta-Jataté with only 11 species. Numbers of species from other geographical units are presented in Table 2. Spatially, Astyanax aeneus and Rhamdia guatemalensis appeared in all regions within Chiapas. Other species with widespread distributions were Poecilia sphenops and the exotic cichlid Oreochromis niloticus (10 and 11 regions respectively). Atherinella alvarezi, Brycon guatemalensis, Dorosoma anale, Dorosoma petenense, and Ictalurus meridionalis were distributed in nine regions, while Aplodinotus grunniens, Gambusia sexradiata, Ophisternon aenigmaticum, Parachromis managuensis, Poecilia mexicana, Pseudoxiphophorus bimaculatus, and Thorichtys helleri were recorded in eight regions.

Discussion
Knowledge of the species richness of continental fishes in Chiapas has increased significantly over recent years compared to previous assessments (e.g. Rodiles- Hernández et al. 2005, Velázquez-Velázquez et al. 2013). The increasing number of known species is the result of collections in new localities, improvement in sampling effort, and larger systematic and taxonomic reviews. For instance, an extensive literature search provided many reports of marine species, principally elasmobranchs, in continental waters of Chiapas by Castro-Aguirre et al. (1999). The large increment in the checklist is due to the inclusion of many elasmobranchs fishes that were included previously in the work of Castro-Aguirre et al. (1999), but that for some reason these records were ignored in more recent accounts of fishes in the continental waters of Chiapas. Castro-Aguirre et al. (1999) reported 41 species of marine fishes including an important number of sharks and sting-rays in the state continental water.
Two species previously reported were removed from the list of species in Chiapas in this study: the American eel (Anguilla rostrata) and the Mexican tetra (Astyanax mexicanus). The American eel was mentioned in the pioneering work of Velasco-Colín (1976), and since then listed in subsequent publications (Lozano-Vilano and Contreras-Balderas 1987, Rodiles-Hernández 2005, Espinosa-Pérez et al. 2011, Velázquez-Velázquez et al. 2013). However, these works do not offer precise geographical locations for these species and there are no vouchered specimens from Chiapas in national or international collections. Records of the Mexican tetra in Chiapas probably contain misidentifications as mentioned by Lozano-Vilano and Contreras-Balderas (1987) and Ornelas-García et al. (2008), thus supporting the absence of this species in Southern Mexico. We have included Important and recent taxonomic changes made in the family Cichlidae by McMahan et al. (2015) and Říčan et al. (2016), the family Poeciliidae by Palacios et al. (2016) and the family Profundulidae by Morcillo et al. (2016).
More than 1000 species of fishes have been reported in the continental waters of Mexico, including freshwater and estuarine fishes (Espinosa-Pérez 2014). The continental fish fauna of the state of Chiapas represents approximately 29% of the continental fish fauna of the entire country of Mexico. This highlights the great diversity of fishes inhabiting continental environments of Chiapas as a result of the region's hydrological wealth. Our results are comparable with those from other southern Mexican states such as Quintana Roo (Schmitter-Soto 1998), Oaxaca (Martínez-Ramírez et al. 2004) and Tabasco (Espinosa-Pérez and Daza-Zepeda 2005).
The native obligate freshwater (primary and secondary) species of Chiapas accounted for only 26% (81) of the state's total species richness. The communities are dominated by peripheral species, many of them permanent (vicarious) residents of the Grijalva-Usumacinta basin (e.g. Aplodinotus grunniens, Eugerres mexicanus, Hyporhamphus mexicanus, Strongylura hubbsi), but the majority are distributed in brackish environments of the Costa-Itsmo and Costa-Soconusco sub-basins. Some of these communities also permeate nearby rivers. In terms of slopes, the Pacific slope houses 68% of the state fish fauna while the Gulf slope houses 33%, and in terms of regional diversity the Usumacinta region is considered one of the most diverse areas of endemism for freshwater fishes in Central America; however, from a biogeographical perspective the entire Central American region has a depauperate freshwater fish fauna compared with the vast diversity of ostariophysan fishes found in North and South America (Miller 1966, Myers 1966, Bussing 1985, Chakrabarty and Albert 2011. This could explain the presence of a great number of peripheral species recorded in the continental environments of Chiapas. This pattern is comparable with other countries of Central America such as Guatemala (Kihn-Pineda et al., 2006), Honduras (Matamoros et al. 2009) and El Salvador (McMahan et al. 2013).
Mexican law protects thirteen freshwater species; however, Rhamdia guatemalensis is quite abundant in Chiapas and possesses a wide distribution through other geographic areas of Mexico and Central America (Miller et al. 2005, Hernández et al. 2015. Its inclusion should be reconsidered in the NOM-059-SEMARNAT-2010. Conversely, we suggest that Mexican laws should consider including Lacantunia enigmatica, Rhamdia laluchensis and Vieja breidohri as protected species on the grounds of their restricted distribution. Since the pioneering work of Lozano-Vilano and Contreras-Balderas (1987), this is the first time the state of Chiapas has been regionalized in a more detailed scale than the three great basins (Grijalva, Usumacinta and Costa). Lozano-Vilano and Contreras-Balderas (1987) proposed seven physiographic regions; however, their proposal was based on physiographic characteristics of landscape relief rather than hydrology. In this study we present a zonation based on the level of hydrological regions (sub-basins), which provides a more robust delineation of the geographical areas for fish species and facilitates a closer examination of the distribution of endemic species. This approach demonstrates that gaps in knowledge of the distribution of species is still quite large and indicates that some portions of the territory remain moderately sampled or unex-plored. For instance, the Usumacinta-Jataté sub-basin, with only 11 species recorded, remains largely unexplored. The detailed regionalization of Chiapas highlights the necessity of increasing sampling efforts in certain zones.
Although hydrological regions Grijalva, Usumacinta and Costa of Chiapas have been used in previous studies to discover endemism in the state (Rodiles- Hernández, 2005, Velázquez-Velázquez et al. 2013, the zonation of our study allows identification of smaller geographic units, permitting us to be more specific in studies of endemism. Thus, the distribution of endemic species in Chiapas includes: Lacantunia enigmatica in Usumacinta-Lacantún, Rocio ocotal in Usumacinta-Lacantún, Thorichthys socolofi in Grijalva-Tulijá and Usumacinta-Lacantún, Tlaloc hildebrandi in Grijalva-Teapa and Usumacinta-Jataté, and Poecilia thermalis in Grijalva-Teapa. Of the 12 units, Usumacinta-Lacantún stands out as it houses three endemic species: Lacantunia enigamatica, Rocio ocotal, and Thorichthys socolofi. Forty years of scientific research on the continental fish fauna of Chiapas has gone a long way since the work of Velasco-Colín (1976). However, this does not seem nearly enough time to completely finish to record the real extend of the state species richness with its distribution. In this work we present distributional data at 12 geographic units. However, although this is the finest distributional scale for the state, a major goal should be to complete distributional data for the 92 existing sub-drainages in the state. Many of these water bodies have never been sampled either for lack of financial resources or because they are located in remote areas of the state.