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Polypeptide synthesis in human fibroblasts infected with DNA-negative mutants of cytomegalovirus.

Two DNA-negative temperature-sensitive mutants of human cytomegalovirus strain AD169 were shown to be deficient in induction of several prominent viral polypeptides when grown in human foreskin fibroblasts under nonpermissive conditions. The use of a monospecific polyclonal antiserum allowed recognition of a further defect in processing of an 'early' viral polypeptide of 135 kD.