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Interactions of Rat Embryonic Hippocampal Neuronal Stem Cell on Gold Nano‐Film Surface

Hippocampal neuronal stem cell culture on the nano‐substrates has introduced new era in nano‐neuroscience research. Neurons were cultured on the gold nano‐film surfaces to see the viability and interactions between neurons and nanoscale surfaces. Embryonic day 18 rat hippocampus tissues were mechanically dissolved into neuronal cells and cultured on top of the gold nano‐flim (GNF) surfaces and preserved at 370 C constant temperatures in a 5% CO2 incubator. These neurons on the GNF surfaces started to grow from day 2 and survived up to 25 days or more. For adhesion and growth of neurons we used an external coating protein, Poly‐D‐Lysine (PDL) layer on the top of the GNF. The extracellular protein PDL acted as an intermediate substance for neurites growth on the gold nano films slips. Without a PDL coating layer showed no or very little growth of neurons on the GNF surfaces. This study is the initial result for implementing the gold nano‐substrates to record the electrical signals from the neurons through gold nano‐films or nano‐wires.