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[Effect of transforming growth factor - beta1 on expression of CD69(+) and CD25(+) T cell induced by allo - corneal epithelial cells in vitro].

OBJECTIVE
To study the effect of transforming growth factor - beta(1) (TGF-beta1) on changes of CD(69)(+) and CD(25)(+) T cell population induced by allo - corneal epithelial cells in culture.


METHOD
The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was identified by using indirect immune fluorescence flow cytometry analysis system.


RESULT
The expression of CD(69)(+) and CD(25)(+) in peripheral T cells was significantly increased after peripheral blood had been cultured with allo - corneal epithelial cells for 3 hours However, the expression of CD(69)(+) was significantly decreased while TGF - beta(1) was added to the culture.


CONCLUSION
TGF - beta(1) significantly inhibits the expression of CD(69)(+) and CD(25)(+) in peripheral T cells induced by allo-corneal epithelial cells in vitro.