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Study on SNP of PIT-1 Gene in Pig

The single nucleotide polymorphisms(SNP) of the pituitary specific transcription factor 1(PIT-1) gene's a 938 bp segment encompassing base 1293 through base 2230 was analyzed by PCR and DNA sequence measuring in miniature pig breeds(Xiang Pig and Bama Pig)and control breeds(Shanghai White and Yorkshire).The results showed that there were many mutations in the fifth intron of PIT-1 gene in the four breeds,and G was replaced by A which occurred 1429th in Xiang Pig and Bama Pig in comparison with DNA sequence published in gene bank,the control breeds(Shanghai White and Yorkshire) did not appeared the mutations.In other hand,there was no mutation in the sixth exon of PIT-1 gene in the four breeds.Conclusion: The 1429th of PIT-1 gene is a candidate locus in gene marker of miniature pig breeds.