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[New bioassay method for measurement of angiotensin II (author's transl)].

Angiotensin II has been shown to induce aldosterone synthesis in man, and to stimulate cortisol as well as aldosterone synthesis in isolated bovine adrenals. The present study has been carried out to determine whether low concentration of angiotensin II stimulates 11-0HCS production in isolated bovine adrenal cells, and whether steroidogenesis by angiotensin II can be utilized for the bioassay of angiotensin II. Isolated cells were prepared by a modification of the Hanig procedure from bovine adrenal inner slices. These cells responded to the addition of valyl-5-angiotensin H by increased production of 11-OHCS. And 11-OHCS levels were determined by a modification of the Silber procedure. Production of 11-OHCS by the isolated cells in response to angiotensin II was increased in parallel with calcium and potassium concentrations of the incubation medium. Net production of 11-OHCS was maximum at the calcium and potassium concentrations 5.08 mM and 8 mM respectively. Addition of bovine serum albumin (BSA) to the medium increased 11-OHCS production, and it was maximum at the BSA concentration of 0.75%. Minimum effective dose of angiotensin II for the stimulation of 11-0HCS production was 0.5ng. (p<0.01). The isolated adrenal cell method is of satisfactory accuracy (X=0.038) for the dose range 5ng-20ng, where response is linearly related to the log of the dose.