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[Observation of filaments of 10nm in the demembraned cytoplasm of Amoeba proteus].

We report here the first observation of 10 nm filaments in a protozoan, Amoeba proteus. These intermediate sized filaments were observed in spread cytoplasmic preparations of amoeba as stable cytoplasmic components over a wide range of pH (5.0-9.0). Although their morphology is grossly similar to the vertebrate intermediate filaments by negative staining, the filaments of amoeba show a characteristic helical structure with a 25 nm axial periodicity and do not display fibrillar projection along their length or at their extremity.