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Stability of Four Steroids in Lyophilised Human Serum

The stability of a preparation of lyophilised serum and its suitability for use as a reference material for routine assays of Cortisol, oestradiol, and progesterone have been studied in four laboratories with a variety of assay systems. Cortisol and oestradiol were also measured by gas chromatography-mass spectrometry. The lyophilised serum was suitable for use in all routine and reference assay methods examined, with between-method variability no greater than that for frozen serum pools. The concentrations of Cortisol, oestradiol, and progesterone were predicted by accelerated degradation studies to decline by 0·01% per annum if the preparations were to be stored at —20°C. The testosterone content of the preparation, determined in one laboratory, provided no evidence for degradation. The preparation can be shipped for use at ambient temperature without deleterious effect.