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[Comparative proteomics of pancreatic cancer].

OBJECTIVE
To identify differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues by comparative proteomics analysis.


METHODS
Six pairs pancreatic tumor tissues and the adjacent noncancerous tissues were obtained and selected for two-dimensional polyacrylamide gel electrophoresis (2-DE) analysis. The differentially expressed proteins were identified using the PDQuest 2-D analysis software. After cutting and enzymolysis, the differentially expressed proteins were analysised by ESI-Q-TOF mass spectrometer. The MS/MS data were acquired and searched in the Swiss-Prot database using MASCOT software. Altered expression of representative proteins was validated by immunohistochemical staining.


RESULTS
A total of 97 points were identified as differentially expressed by two-dimensional gel electrophoresis, of which 31 were successfully identified by ESI-Q-TOF mass spectrometer. Among the proteins identified by mass spectrometer, 23 were upregulated and the other 8 were downregulated. These proteins were involved in different functional processes and protein families: glucolysis, hyaluronidase, I, II-phase metabolic enzymes, metalloproteinase, cytoskeleton, Ca2+ metabolism and so on. Immunohistochemical staining revealed an overexpression of HYAL1 and low expression of CYP2C8 and GSTM2 in pancreatic tumor.


CONCLUSION
The differentially expressed proteins between pancreatic tumor tissues and the adjacent noncancerous tissues can be identified by comparative proteomics approach. The selected proteins will provide a valuable clue and help for further research.