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Polymorphism of Natural Resistance-Associated Macrophage Protein 1 Gene in Hy-Line Chicks infected Salmonella gallinarum

Salmonella gallinarum (S. gallinarum) is the causative agent of fowl typhoid, a severe systemic disease of chickens that results in high mortality amongst infected flocks. Due to its virulence, the immune response to S.gallinarum is poorly characterized. The natural resistance-associated macrophage protein 1 (NRAMP1) affects host innate immunity to intracellular bacteria because of its ability to transport divalent cations in late endosome/lysosomes. The objectives of this study were to identify single nucleotide polymorphisms of the NRAMP1 gene in Hy-Line chicks by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). A PCR-RFLP method was developed to identify a SacI polymorphism for Ser³??. To investigate associations between an NRAMP1 SNP and resistance against S.gallinarum, two NRAMP1 alleles of a T and C were analyzed to 3 genotypes, TT (802 bp), TC (802, 723 bp and 79 bp), and CC (723 and 79 bp). We evaluated the effect of 3 genotypes, such as TT(66 %), CC (36.9 %) and TC (41.9 %) alleles on mortality after S. gallinarum challenge.