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S-100 Protein Synthesis by Isolated Polyribosomes from Rat Brain

The radioactive proteins synthesized in a cell-free system and released from polyribosomes from rat brain were analyzed for the presence of a protein found only in the nervous system. Polyribosomes from rat liver were also studied to demonstrate the organ specificity of the radioactive product synthesized in vitro. The S-100 protein constituted 0.15 percent of the radioactive proteins released from brain polyribosomes.