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[The effect of leptin on the proliferation of airway smooth muscle cells in rats].

AIM
To explore the effect of leptin on the proliferation of rat airway smooth muscle cells(ASMCs) and its possible mechanism.


METHODS
ASMCs were derived from rat airway tissue and cultured in vitro. RT-PCR and Western blot were used to verify the expression of leptin receptors on ASMCs. And then ASMCs were treated with leptin at different concerntrations (0-100 μg/L) for different periods of time (1-72 h), respectively. Cell proliferation was measured by CCK-8 assay. The expression of p-ERK and PI-3K in ASMCs were quantified by Western blot after being treated with leptin at different concentrations.


RESULTS
Leptin of various concentrations was used to treat different time could promote the proliferation of ASMCs in dose-dependent manner (r=0.837, P<0.01)and time-dependent manner (r=0.874, P<0.01). Western bot indicated that the expression of p-ERK and PI-3K increased with the increase of leptin concerntrations (P<0.05). The expression of p-ERK and PI-3K was a positive correlation with the concerntrations of leptin (r=0.793, P<0.01; r=0.746, P<0.01).


CONCLUSION
Leptin can significantly stimulate ASMCs proliferation and its mechanism may be correlated to activation of p-ERK and PI-3K.