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Novel Reassortant Influenza A(H1N2) Virus Derived from A(H1N1)pdm09 Virus Isolated from Swine, Japan, 2012

We isolated a novel influenza virus A(H1N2) strain from a pig on January 13, 2012, in Gunma Prefecture, Japan. Phylogenetic analysis showed that the strain was a novel type of double-reassortant virus derived from the swine influenza virus strains H1N1pdm09 and H1N2, which were prevalent in Gunma at that time.

I nfluenza A viruses can be transmitted between humans, swine, and birds; virus subtypes have the potential to reassort and generate new viruses by cross-breeding in the various hosts (1). For example, influenza A subtype H1N1 viruses reassorted in swine, and the resulting swine influenza viruses (SIVs) were transmitted to humans. The reassorted combinations have resulted in pandemic viruses as well as low-pathogenicity viruses with low transmissibility among humans. Similarly, seasonal human subtypes of influenza are transmissible to swine (2). In 2009, a novel strain of the H1N1 SIV subtype emerged and was associated with a pandemic (3,4). The virus, later termed influenza A(H1N1)pdm09, hereafter referred to as pH1N1, was confirmed as a reassortant virus resulting from cross-breeding of a European avian subtype H1N1 virus and a North American triple reassortant virus (5). Subsequently, other strains reassorted from the pH1N1 virus (6)(7)(8). We report on an isolated new reassortant H1N2 SIV derived from the pH1N1 virus and SIVs originating in Japan.

The Study
We collected 109 nasal swab samples from pigs for swine influenza surveillance during November 2011-February 2012. Nasal swab samples were collected from healthy pigs, 6 months of age, at an abattoir in Gunma Prefecture, Japan. All samples were inoculated onto MDCK cells (9).
All cell culture supernatants were tested by using a hemagglutination assay of a 0.7% solution of guinea pig erythrocytes (9). To determine the subtype of the isolate, a hemagglutination inhibition assay was performed by using ferret antiserum for A/California For full genome sequencing of the influenza A/swine/ Gunma/1/2012 strain, we conducted reverse transcription PCR (10). Segment-specific primers used for amplification and sequencing are shown in online Technical Appendix Figure, panel A (wwwnc.cdc.gov/EID/article/19/12/12-0944-Techapp1.pdf). Phylogenetic analysis of the nucleotide sequences was conducted by using MEGA version 5 software (www.megasoftware.net) and Tree Explorer version 2.12 (http://en.bio-soft.net/tree/TreeExplorer.html) (11). Evolutionary distances were estimated according to the Kimura 2-parameter method (12). The phylogenetic trees of hemagglutinin (HA) and neuraminidase (NA) genes were constructed by using the neighbor-joining method (13). In addition, phylogenetic trees based on the matrix protein, nucleoprotein genes, nonstructural protein, polymerase acid, polymerase basic 1, and polymerase basic 2 were constructed by using the neighbor-joining method. The reliability of the trees was estimated with 1,000 bootstrap replications. GenBank accession numbers assigned to the gene sequences of the analyzed strain are the following: polymerase basic 2 (AB731582), polymerase basic 1 (AB731583), polymerase acid (AB731584), HA (AB731585), nucleoprotein (AB731586), NA (AB731587), matrix protein (AB731588), and nonstructural protein (AB731589).
Phylogenetic trees based on HA and NA gene sequences are shown in the Figure, ; Table). These results suggest that the A/swine/Gunma/1/2012 strain was a new reassortant of the H1N2 SIV subtype derived from the pH1N1 virus.
We isolated 1 strain in this study. The samples (109 nasal swabs) were collected from different pig farms ≈60 km apart. The epidemiologic association may be low among the samples, because the quarantine inspection system is well established in Japan. All samples were collected from pigs 6 months of age; therefore, the potential for infection with the virus could have been low. Additional and larger studies investigating the emergence of the parent virus of the strain may be needed.

Conclusions
Vijaykrisna et al. found a new reassortant virus among avian-type, swine-type, and pH1N1 viruses (6). In addition, Monero et al. reported a new reassortant virus between SIV, identified in Italy, and pH1N1 viruses (7). Thus, pH1N1 virus and other types of influenza viruses can be reassorted. However, to our knowledge, reassortant H1N2 SIV strains derived from pH1N1 virus in Japan have not been identified before this report. Although the transmission of SIVs to humans has been reported sporadically, the infectious nature of this reassortant H1N2 strain among humans is unknown. The emergence of a novel H1N2 SIV strain raises further concerns about whether the virus will generate further genetic reassortments and gain virulence. Systematic influenza virus surveillance in pigs and humans should be considered.   "milky white threads" in the muscles of a mouse, which for years were known as "Miescher's tubules." In 1882, Lankester named the parasite Sarcocystis, from the Greek sarx (flesh) and kystis (bladder). Scientists were unsure whether to classify the species as protozoa or as fungi because only the sarcocyst stage had been identified. In 1967, crescent-shaped structures typically found in protozoa were seen in sarcocyst cultures, and it was determined to be a protozoan, a close relative of Toxoplasma spp. In 1969, A. M. Mandour described a new species of Sarcocystis in rhesus macaques, which he named Sarcocystis nesbitti, after Mr. P. Nesbitt, who saw the trophozoites in stained smears. Snakes are now known to be the definitive hosts of S. nesbitti, and several primates, including humans, can be intermediate hosts.