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Acetylglutamate synthase from Neurospora crassa: structure and regulation of expression

A DNA clone which complemented an arg‐14 mutation of Neurospora crassa was isolated by sib selection from a cosmid library (pMOcosX). Southern and restriction‐fragment‐length polymorphism (RFLP) analysis confirmed that the cloned DNA contained the arg‐14 gene. The arg‐14 gene was identified as the structural gene for acetylglutamate synthase by immunodepletion of enzyme activity with antibodies prepared against an arg‐14 fusion protein and by the thermal instability of acetylglutamate synthase in a temperature‐sensitive arg‐14 mutant. The fungal acetylglutamate synthase has little sequence homology to its bacterial counterpart, unlike other arginine biosynthetic enzymes. Expression of the arg‐14 gene is regulated by cross‐pathway control similar to many amino acid biosynthetic genes. However, expression of acetylglutamate synthase occurs throughout the developmental growth cycle, unlike other arginine biosynthetic enzymes.