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Vasodilatory Effect of Adrenomedullin in Mouse Aorta

Human adrenomedullin (AM) and human calcitonin gene-related peptide (CGRP) produced a concentration-dependent relaxation in mouse aorta, precontracted with noradrenaline. EC50 values for AM and CGRP were 9.8 ± 2.4 and 4.2 ± 0.1 nmol/l, respectively. AM-mediated vasorelaxation was partially (3-fold) shifted by AM(22–52), the C-terminal AM fragment, but not by CGRP(8–37), a selective CGRP1 antagonist. Both AM(22–52) and CGRP(8–37) failed to inhibit CGRP-mediated vasorelaxation of mouse aorta rings. Binding of rat [125I]AM to these membranes was specific. Both human AM and AM(22–52) displaced rat [125I]AM binding in a concentration-dependent manner with IC50 values of 12.0 ± 4 and 19.4 ± 8 nmol/l, respectively. In contrast, both human CGRP and CGRP(8–37) were weak in displacing [125I]AM binding. Very little specific binding was observed with [125I]CGRP. In conclusion, the data presented here demonstrate that the mouse aorta displays AM receptors that mediate vasorelaxation.