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Stability Characteristics of Freeze-Dried IVRI-CSF-BS Classical Swine Fever Vaccine

Freeze-drying (lyophilization) of biologically active materials including attenuated live virus vaccine is an well accepted method for ensuring stability and preserving the vaccine potency. As freeze- drying does not ensure complete stability, studies for stability of freeze-dried vaccines after exposure at different temperatures are required. Heat stability of classical swine fever (CSF) virus vaccine is also urgently needed in tropical countries including India where the disease is still endemic. In this study, the thermostability of freeze- dried vials of live attenuated CSF vaccines were investigated at 4℃ and 25℃ for 30 days. This lyophilized vaccine containing combination of sucrose, lactose and lactalbumen hydrolysate gives good heat stability and viral infectivity was reduced by a factor of 0.2 to 1 Log10TCID50/mL at the end of freeze drying. In this experiment, we have studied storage stability of lyophilized classical swine fever vaccine produced at Division of Biological Standardization, IVRI using IVRI-CSF-BS vaccine strain which can be stored at -20℃ for more than a year without any loss. Stability at 4℃ and 25℃ may ensure easy transportation and minimize expense related to that.