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Radioassay of cortisol and corticosterone by a modified competitive protein-binding method.

A competitive protein-binding radioassay using adsorption was evaluated for the individual determination of cortisol and corticosterone. The competitive protein-binding assay using fuller's earth to separate bound and free cortisol was tested and found to offer no specificity for cortisol in favor of corticosterone. The introduction of a carbon tetrachloride/water partitioning procedure to separate corticosterone and cortisol made possible the individual determination of corticosterone and cortisol using the competitive protein-binding radioassay. The modified competitive protein-binding radioassay was evaluated as to precision, accuracy, and specificity; normal values of cortisol and corticosterone in human plasma were determined.