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[Establishment of immunohistochemical technique for polyamine and its application in research of lung cancer].

Antiputrescine, antispermidine and antispermine were used to detect polyamine in normal and cancer tissues of lung by PAP method. Sections of lung cancer, which were known to have high concentration of polyamine, were used as positive control. Results showed highly significant difference in the staining between the experimental and control groups. Immunohistochemical assay further demonstrated that polyamine located mainly in the nucleus, nucleolus and cytoplasm of the cancer cells and was evenly distributed. The staining results of normal lung tissue and epithelium of bronchi were negative or weakly positive. The morphological finding of this experiment is considered important for further study on the correlation of polyamine with the differentiation, proliferation and malignant change of cells.