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In Vitro Formation of Homocysteine in Whole Blood in the Presence of Anticoagulants

reference interval value. In addition, 46 serum samples from healthy individuals were tested using the EFC-cAMP method (mean 94.4%, median 94.5%, and SD 6.8%). Results for the normal samples were 80% to 106% of the control (data not shown). This EFC-cAMP assay is a nonradioactive surrogate for RIAcAMP for studying antibody-mediated increases in cAMP concentrations in response to activation of the G-coupled TSH receptor.