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Procedure for the isolation of avian adeno and avian adeno-associated viruses.

The avian adenoviruses (AV) are common infectious agents of chickens. They have been isolated from chickens suffering specific diseases, but more often have been associated with inapparent infections. Their role in avian diseases is unclear. The adeno-associated viruses (A-AV) are small defective DNA viruses that are likely as common in poultry as the AV. Being defective, they can multiply only in cells coinfected with a "helper" virus. Experimentally, it appears that the AV are the only agents that can supply the complete "helper" function, that is, assist in the production of infectious virus (1). The importance of the A-AV as a modifier in avian diseases has not been investigated. For proper assessment of the importance of these agents in disease outbreaks their presence in a flock must be determined. The isolation procedures presented here have been found to be sensitive and also convenient enough to be useful in the diagnostic laboratory.