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Salt‐ and pH‐dependent hemagglutination with Kasba virus, a member of the Palyam serogroup of the genus Orbivirus

Kasba virus grown in BHK21‐WI2 cells was tested for hemagglutination (HA) with erythrocytes of a variety of species at 4°C, 25°C and 37°C. HA was observed at all temperatures with cattle, sheep and goat but not with swine, chicken, and goose erythrocytes. The HA was dependent on not only the NaCl concentration but also the pH of the diluent. The HA titer significantly improved by increasing the NaCl molarity to 0.6 M and standardizing pH to 7.5. The HA titer was 16‐ or 32‐fold higher in 0.4 M or 0.6 M solution than in 0.2 M solution of not only NaCl but also several other salts. The HA reaction was inhibited by specific antibody.