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Isolation and partial purification of hemolytic toxin from sea anemone, Stoichactis helianthus.

The isolation of a toxic principle(s) from the sea anemone, Stoichactis helianthus, was effected by subjecting the anemones to a freeze-thaw cycle followed by immersion in deionized water. The crude toxic material was removed from the aqueous phase by precipitation with acetone and was partially purified by gel filtration. The partially purified toxin has a molecular weight greater than 5000 and an LD50 of 0.25 mg/kg in mice, and it causes complete hemolysis of a 1% suspension of sheep red blood cells at 0.5 μg/ml. The toxicity in mice is destroyed upon interaction of the partially purified toxin with concanavalin A.