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HIGH PERFORMANCE REVERSED-PHASE LIQUID CHROMATOGRAPHY USING NOVEL CIM RP-SDVB MONOLITHIC SUPPORTS

Monoliths have already proven to be efficient chromatographic supports for the separation of various types of molecules. In this paper, the characterization of the novel reversed-phase support, CIM® RP-SDVB disk monolithic column is presented. Using a 3 mm long RP-SDVB disk monolithic column, excellent separation of proteins within a very short time was achieved. The pressure drop observed on the column was considerably low (few bars), even at flow rates of the mobile phase up to 30 mL/min. Due to the low pressure drop, the use of high flow rates was preferred since they did not influence the quality of the gradient separation. The separation of test proteins was performed within only 14 seconds; faster separations were limited by the configuration of the HPLC system. Furthermore, RP-SDVB disk monolithic columns were applied for fast separation of peptides. Five peptides of different lengths and composition were successfully separated in a very short time. Finally, the preparative purification on the laboratory scale of the complex sample of oligodeoxynucleotide within a range of 1 minute demonstrates practical applicability of these columns.