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Effect of Upemla Artifact on HbAI 0 Concentration In 8 Samples

densitometer, which also calculated the percentage of HbAic. Samples that initially produced the lipemia artifact were centrifuged for 10 miii at 1000 x g. The lipemic plasma was decanted and replaced with an equal volume of isotonic saline (9 g/L NaC1). The samples were mixed and the electrophoresis procedure was repeated. To evaluate the effect of this treatment, we used the saline replacement procedure with several samples having various HbA10 concentrations and normal-appearing nonlipemic plasma. The IThAic concentration was unaffected, the average change of concentration in these samples before and after the saline replacement treatment being 0.0%. Table 1 summarizes the error that occurs when attempting to quantify HbA1C in the presence of the interfering lipemia peak. The lipemia interference was evaluated by scanning the gel and either including or exdudung the artifact in the area under the HbA1C peak. Next, the lipemia was removed from the sample by the replacement technique, and the HbA1C value was determined in the absence of the interfering lipemia peak. Interference was calculated by comparing the calculated HbA1C values with the value measured when the lipemia artifact was removed by the saline replacement technique. When the artifact was included in the calculated area under the HhAC peak, the HbA1C values were falsely elevated. The positive error ranged from 5.5% to 34.8% (average, 18.2%). When the artifact was electronically excluded from the ic peak, the HbA1C values were falsely decreased. The negative error ranged from 0.0% to -27.5% (average, 15.7%). The saline replacement procedure is simple and easy to use and allows a more accurate measurement of HbA1C by the Beckman Diatrac electrophoretic procedure.