{'fraction_non_alphanumeric': 0.02711864406779661, 'fraction_numerical': 0.010169491525423728, 'mean_word_length': 6.031746031746032, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Antibodies to Postsynaptic PKC Substrate Neurogranin Prevent Long‐term Potentiation in Hippocampal CA1 Neurons

Both pre‐ and postsynaptic protein kinase C have been implicated in long‐term potentiation. Neurogranin (also known as BICKs and RC3) is a neuronal postsynaptic protein kinase C substrate. In the present study we injected monoclonal IgGs that recognize the protein kinase C phosphorylation site in neurogranin and B‐50 (GAP‐43), and that have been shown to inhibit protein kinase C‐mediated B‐50 phosphorylation, through a whole‐cell clamp pipette into CA1 pyramidal neurons in rat hippocampal slices. Injection of neurogranin IgGs, but not of control IgGs, prevented the induction of tetanus‐induced long‐term potentiation without affecting posttetanic potentiation. Our results suggest that neurogranin is involved in mechanisms of activity‐dependent synaptic plasticity.