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RNA interference mediated YAP gene silencing inhibits invasion and metastasis of human gastric cancer cell line SGC-7901.

BACKGROUND/AIMS
This study aimed to investigate the effects of yes-associated protein (YAP) silencing on invasion, migration, anchorage-independent growth and angiogenesis in the human gastric cancer cell line SGC-7901.


METHODOLOGY
SGC-7901 cells were transfected with a shRNA lentivirus vector targeting YAP gene. After puromycin selection of stable YAP-silenced SGC-7901 cell lines, the expression of YAP mRNA and protein were detected using quantitative real-time PCR (qRT-PCR) and western blotting. A transwell invasion assay, wound healing assay and colony formation assay were used to analyze the invasion, migration and anchorage-independent growth effect of RNA interference (RNAi) on YAP expression. The angiogenic cytokines, vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), were measured by qRT-PCR and western blotting.


RESULTS
The results of the qRT-PCR and western blotting showed an obvious inhibition of YAP mRNA and protein expression in the YAP-shRNA group. Moreover, the inhibition of YAP expression clearly impeded SGC-7901 cell migration, invasion and anchorage-independent growth. Furthermore, VEGF and FGF-2 were also downregulated.


CONCLUSIONS
YAP gene targeting by RNAi can inhibit the migration, invasion, anchorage-independent growth and angiogenesis ability of SGC-7901 cells. YAP may be a potential target for the treatment of human gastric cancer.