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Tannin biodegradation by tannase from Serratia marcescens: optimization of production by response surface methodology and its partial characterization

Abstract Tannin acyl hydrolases catalyzes the hydrolysis of tannins and can be used in different industrial sectors such as in food, beverage, pharmaceutical and chemical industries. The aim of the current study was to assess tannase production by Serratia marcescens. The experiments showed activity variation between 0.25 and 59.05 U mL−1 in Plackett-Burman design. The production was optimized after applying the Doehlert design, and an activity of 78.79 U mL−1 was obtained, thus corresponding to an increase of 315.16 times. The enzyme had a peak activity at pH 6.0 and 40 °C. It was inhibited in the presence of metal ions such as Cu2+, Fe2+ and Ba2+ and was activated by Na1+ and K1+. Results indicate the feasibility of using tannase from S. marcescens in biotechnological processes, such as in beverages manufacturing and gallic acid production.