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Comparative Evaluation of Remineralization Potential of Casein Phosphopeptide- Amorphous Calcium Fluoride Phosphate and Novamin on Artificially Demineralized Human Enamel: An In vitro Study

Aim: This study aimed to quantitatively compare the remineralization potential of casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) and Novamin on artificially induced enamel subsurface lesions using scanning electron microscope-energy dispersive X-ray (SEM-EDX). Materials and Methods: Enamel specimens were prepared from seventy caries-free premolars and were kept for demineralization for 4 days. Following demineralization, enamel specimens were assigned to three groups: (1) Control group, (2) Group with CPP-ACFP-containing toothpaste, and (3) Group with Novamin-containing toothpaste. Further, both the experimental groups (CPP-ACFP group and Novamin) were then divided into three subgroups based on the duration of re-mineralization as follows: (a) 7 days, (b) 14 days, and (c) 21 days. Enamel specimens of experimental groups were then subjected to SEM-EDX to evaluate mineral content after treating with CPP-ACFP and Novamin. Results: Both the experimental groups showed very high significant differences between Ca/P ratios of remineralized samples. Conclusion: Even though both CPP-ACFP and Novamin showed remineralization potential, remineralization was found to be higher in the samples treated with CPP-ACFP.