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Validating Field Regeneration Capacity for Selected Accessions of Gossypium hirsutum Using Callus Induction and Regeneration Capacity


 Gossypium hirsutm undergoes a rapid clonal propagation to regenerate a mature plant through tissue culture. In this research, cotton leaf regeneration level for 21 accessions in the field (new leaves) was observed after the first harvest, and a comparison between field regeneration level and callus induction with its regeneration capacity (new shoots and roots) for the same 21 accessions was carried out. During the flowering stage of Gossypium hirsutum, biochemical (Proline), physiological (chlorophyll and carotenoid content) analysis was carried out. Phenotypic observations (plant height, leaf area, fresh leaf weight, dry leaf weight, flower and boll number) were also carried out on 21 accessions for each accession. Callus induction and regeneration capacity of roots and shoots for hypocotyl, cotyledons and shoot tip tissues was used to validate field regeneration capacity through analysis of variance. ZS061, LuMian378, JiMian863, and ZS065 have highest drought tolerance while ZhongMianSuo24, LiaoYangDuoMaoMian, and BeiZheGongSheMian have the lowest tolerance to drought stress. Accessions with both field and callus regeneration capacity were identified.