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Simultaneous determination of poly- and perfluoroalkyl substances and organophosphorus flame retardants in serum by ultra performance liquid chromatography-tandem mass spectrometry.

RATIONALE Poly- and perfluoroalkyl substances (PFASs) and organophosphorus flame retardants (OPFRs) are two types of emerging organic pollutants with potential human health hazards. Here, a rapid and sensitive method was developed for the determination of sixteen PFASs and seven OPFRs in human serum by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).


METHOD
After optimized the chromatographic and mass spectrometric conditions, respectively, 100 μL of serum sample was liquid-liquid extracted using 8 mL methyl tert butyl ether. The 23 targets were quantified within 8 mins. All targets were quantified by isotope internal standard method in both negative-ion mode and positive-ion mode by UPLC-MS/MS.


RESULTS
Method was validated in terms of sensitivity, linearity, precision, and accuracy. The limit of detection ranged between 0.004 and 0.650 ng/ml. Recoveries ranged from70.0% to 118.9% with a relative standard deviation lower than 20%. The developed method was successfully applied to analyze targeted analytes in human serum samples. 13 of 23 analytes were detected in over 50% of samples.


CONCLUSION
A sensitive and rapid method was developed to quantify 16 PFASs and 7 OPFRs in serum. Sensitivity, linearity, recovery, and precision were validated and found satisfactory. This method can be a valuable tool for evaluation of exposure to both PFASs and OPFRs with high separation efficiency and sensitivity.