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Quick preparations of human parvovirus B19 microarray probes using PCR.

OBJECTIVE
To prepare DNA microarray probes for the detection of human parvovirus B19. Method Specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome. The PCR products were cloned into the pMD-18 T vector.


METHOD
Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19.


RESULT
Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19.


CONCLUSION
PCR amplification of the conserved and specific human parvovirus B19 genes is simple and effective to prepare the desired probes.