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Amperometric sensing of organophosphorus pesticides based on covalently attached multilayer assemblies of diazo-resin, prussian blue single-walled carbon nanotubes, and acetylcholinesterase

A novel organophosphorus pesticides (OPs) biosensor was fabricated based on electrostatic self-asembly in combination with in situ photo-cross-linking of acetylcholinesterase (AChE) on prussian blue (PB) deposited on the single-walled carbon nanotubes (PB-SWCNTs) backbone. The biosensor showed high sensitivity in the linear concentration range from 1.0×10 to 10×10 g·L, and the detection limit of malathion and methyl parathion were 3.11×10 g·L and 1.88×10 g·L, respectively. In addition, the developed biosensor demonstrated to be highly reproducible with R.S.D. values of 4.59% for 5 successive amperometric calibrations using the same surface. When detecting malathion in real samples, the recoveries for the AChE biosensor ranged from 96%–105%, indicating that this had excellent potential for practical application. More importantly, the results exhibited that thus method greatly improved biosensors stability in comparison with electrostatic interaction attributed to the conversion of weak ionic bonds to strong covalent ones for enzyme immobilization by the proposed strategy. It was a simple, cheap and table method to detect OPs and this method might pave the way for the sensitive, simple detection of different analytes without the need for expensive instrumentation.