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Production of citric acid in batch culture

Work has been carried out on the production of citric acid in batch culture using Aspergillus foetidus. It is shown that the dry weight increases throughout the fermentation. The supply of nitrogen is exhausted early and the subsequent increase in dry weight is due to accumulation of carbon by the cells. It appears that the exhaustion of nitrogen is a necessary prerequisite for the production of citric acid, which is produced by carbon‐storing cells. The pH history in the early part of the fermentation did not affect the final yields.