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On the Standardisation of the Direct Spectrophotometric Bilirubin Determination

The influence of different albumin solutions and of the molar bilirubin/albumin ratio on the bilirubin concentration determined by the method of Hertz et al was studied. There is good agreement between the true-bilirubin concentrations and those obtained by the Hertz method for bilirubin-HSA and bilirubin-serum solutions. Moreover the observed concentration is scarcely influenced by the molar ratio. Bilirubin standards in HSA-solutions can thus be used to standardise this method. Errors are introduced by preparing standards in BSA-solutions as the absorption spectrum of bilirubin-BSA is different from that of bilirubin-HSA.