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Some modifications on freeze‐fracture replica preparation

Freeze‐fracture replicas are freed from lipid material by saponification in a hot sodium hydroxide solution and subsequently rinsed with 10% ethanol in distilled water. All manipulations are carried out with a glass bead fashioned onto a pasteur pipette. A stable, two‐layered plastic support film has been developed to allow the use of grids with larger openings so that more of the specimen is observable.