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Physiological Characters Analysis and Gene Fine Mapping of Green-revertible Albino Mutation Line Bai 784

In order to study the physiological characters and relative genetic mechanism of green-revertible albino mutation line Bai 784,the phenotype and physiological characters were studied under different temperature. The results showed that the albino phenotype was controlled by temperature. Under the tem-perature of 20 ℃,22 ℃,24 ℃,pigment synthesis of the mutant seedling was hindered,and the mutant seedling showed albino phenotype. Compared with the control,the content of chlorophyll a,b significantly decreased by 95. 18%,86. 86% respectively under 20 ℃ for 10 d; the content of chlorophyll a,b and carotenoid significantly decreased by 96. 19%,93. 25%,92. 76% respectively under 22 ℃ for 14 d; the content of chlorophyll a,b and carotenoid decreased by 86. 17%,91. 67%,95. 30% respectively under24 ℃ for 12 d; the mutation line turned green,and the chlorophyll a,b and carotenoid content decreased by 27. 63%,25. 70%,27. 17% respectively under 26 ℃ for 12 d; the mutant leaf color was nearly the same as the control under 28 ℃ for 12 d,and there was no difference in the chlorophyll a,b and carotenoid content between mutant and control,indicating that 24- 26 ℃ was the sensitive temperature for albino of leaf. The gene fine mapping was done with F2 mapping population of Bai 784 × Zhengzhouzaojing,and the results showed that the albino phenotype was controlled by a single nuclear recessive gene( Abt6),which was mapped on chromosome 6 between SSR markers RM5998 and RM6071 with a genetic distance of 2. 4 c M and 9. 7 c M respectively. Screening F2 and F3progenies for lines whose marker genotype was heterozygous in target interval,with which and some new In Del markers the target gene was delimited to a 278 kb interval between Y15420 and Y15648.