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Improvement of cold tolerance by knockout of DWF4 gene in Arabidopsis

A T-DNA deletion mutant dwf4 of Arabidopsis was used as material to investigate the role of DWF4 gene in cold tolerance in plants by comparing the phenotypic performance and physioligcal characteristics between dwf4 mutant and wild type seedlings. The dwf4 mutant knockout of DWF4 gene exhibited an increased tolerance to cold ness resulting from lower electrical conductivity,higher chlorophyll content,and higher concentrations of soluble sugar and proline and higher expression levels of RD29 A and COR47 compared with wild type. The higher levels of peroxidase genes Prx22 and Prx698 in dwf mutant effectively inhibited the harm caused by the increased reactive oxygen species. Taken together,it was suggested that DWF4 gene might play a negative role in response to cold stress in Arabidopsis.