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Genetic Stability in Long-Term Micropropagated Plants of Gentiana Kurroo—An Endangered Medicinal Plant

The present investigation was carried out to study genetic stability of micropropagated plants of Gentiana kurroo using randomly amplified polymorphic DNA (RAPD) and karyotype analysis. A large number of micropropagated plantlets of G. kurroo developed from nodal segment explants started more than 10 years ago were assessed for genetic variations. Similarly, the donor mother plant maintained in the arboretum was also subjected to genetic analysis. Out of 20 RAPD primers, 5 displayed the same banding profile within all the micropropagated plants and donor mother plant. The karyotypic analysis showed no chromosomal variations in the plants micropropagated through nodal segment culturing. High multiplication rate of healthy plant material associated with molecular and karyotypic stability ensures the efficacy of the protocol to be used across a long period for in-vitro propagation of this important medicinal plant species.