{'fraction_non_alphanumeric': 0.052083333333333336, 'fraction_numerical': 0.05803571428571429, 'mean_word_length': 4.795977011494253, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Matrix Metalloproteinase ( MMP ) Gene Polymorphisms in Chronic Periodontitis : A Case Control Study in Indian Population

Chronic periodontitis (CP), a commonest form of inflammatory oral disease. Matrix metalloproteinases (MMPs) plays a pivotal role in progression of CP by degrading gingival tissue and its remodeling. In this literature we conducted a case-control study to investigate a possible association of single nucleotide polymorphism (SNP) of MMP genes and their interaction with chronic periodontitis in Indian population. A total of 357 DNA samples from venous blood have been isolated, 157 identified chronic periodontitis patients and 200 healthy individuals. Genotyping of six MMP genes (MMP1, MMP3, MMP7, MMP8, MMP12 and MMP13) was done by PCR followed by Sanger method of sequencing. All statistical analysis was performed by SPSS v16.0, R package (SNPassoc). Gene-gene interaction was evaluated by MDR 3.0.2. Frequency of 6A allele of MMP3 1715A-6A gene polymorphism (36%) and G allele of MMP8 +17G-C gene polymorphisms (34%) are higher in CP population compared to HC population (19% and 24% respectively). A significant association of T allele of MMP8 -799C-T gene promoter polymorphism was found with CP (OR= 2.95, 95%CI= 2.16-4.04, p<0.0001). Genotypic frequency of MMP12 -82A-G polymorphism is associated to CP risk while its allelic distribution is not (OR=1.32, 95%CI= 0.93-1.88, p=0.129). Gene-gene interaction showing the best cross validation (CV) consistency model i.e. is MMP1 -519AG X MMP7 -181A-G X MMP8 -799C-T polymorphisms with value of 9/10. This gene-gene interaction showing significant association with CP increased susceptibility. The findings suggest the significant association of MMP8 -799C-T polymorphism with CP increased susceptibility. Allelic distribution of MMP8 +17G-C and MMP3 -11715A-6A polymorphisms revealed their protective role towards decreased risk of CP. MMP1 -519A-G and MMP7 -181A-G polymorphisms are showing combinatorial synergistic effect on CP risk.