{'fraction_non_alphanumeric': 0.03453453453453453, 'fraction_numerical': 0.0, 'mean_word_length': 4.902654867256637, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
NMR analysis of enzyme-catalyzed and free-equilibrium mutarotation kinetics of monosaccharides.

We have exploited the saturation difference (SD) NMR technique to investigate the mutarotations of two sugars, l-fucose and d-ribose, at equilibrium. The KM and kcat values of Escherichia coli FucU-catalyzed mutarotation of l-fucose have been determined by this technique, and the values of KM for alpha- and beta-forms are shown to be the same. Similarly, the spontaneous mutarotation of d-ribose was investigated by using the same method, and it was found that d-ribose has an exceptionally high spontaneous alpha-to-beta conversion rate only between the furan forms.