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Coumarins from Daphne feddei and their potential anti-inflammatory activities

Chemical examination of the methanolic extract from the stem bark of Daphne feddei led to the isolation of three new dicoumarin glucosides (1–3), and eight known coumarins, dicoumarins and dicoumarin glucosides. Their structures were elucidated by extensive analysis of spectral data and comparison with the literature values. All compounds were tested for inhibitory activity against lipopolysaccharide-induced NO production in RAW 264.7 macrophages, and compounds 4 and 5 showed potent inhibitory activity with IC50 values of 0.161 and 0.127 μM, respectively.