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Effect of Metabolic Inhibitors on Bone Lipid Synthesis from Radioactive Bases

Iodoacetate at 10 -3 M and 10-4 M concentrations was found to strongly inhibit incorporation of both 14C-choline and 3H-inositol into phospholipids of calvaria from newborn rats; it had somewhat less effect on ethanolamine incorporation and almost no effect on serine incorporation. Similar though less dramatic results were observed with cyanide or dinitrophenol at similar concentrations.