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Survival of microorganisms under simulated space conditions.

Monocellular layers of stationary phase cells of Escherichia coli B/r were exposed to high vacuum of 10(-6) Torr at different temperatures. After simultaneous irradiation with UV of 254 nm or X-rays, the survival was tested as colony forming ability. In vacuum the sensitivity to UV and X-rays was enhanced compared with the controls at atmospheric pressure. Of UV-inactivation in vacuum no photoreactivation was found.