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[Alternation of intracellular Ca2+ caused by streptomycin in the isolated cochlear OHC].

Stained with fluorescence, the intracellular free calcium concentration (Ca2+) of the cochlear out hair cell (OHC) isolated from guinea pigs was measured using ACAS 570. Streptomycin caused a remarkable rise in Ca2+ and reached peak value in 60s, decreased gradually after lasting for 60s in that value. The Ca2+ fell back to primary level in another 100s and followed by a slight drop. The distribution of free calcium in the OHC was showed. The rise of intracellular free calcium in the OHC was assumed to be a period in the ototoxicity caused by aminoglycoside antibiotics.