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Genetic Analysis of Resistance to Sudden Death Syndrome Caused by Fusarium virguliforme in Hartwig Soybean

Host resistance is the most effective strategy for soybean (Glycine max) sudden death syndrome (SDS) management. The objective of this study was to determine the inheritance of resistance to SDS in soybean cv. Hartwig under greenhouse conditions. One-hundred and fifty F2:3 lines derived from the cross of the SDS-resistant cultivar ‘Hartwig’ with the SDS-susceptible cultivar ‘Camp’ were inoculated with sorghum seeds colonized with a F. virguliforme reference isolate from Arkansas (AR-171). The experiment was arranged in a randomized complete-block design with two replications. Each block included a pot of each F2:3 line and two pots of each parent with eight plants per pot. The experiment was repeated to give a total of four replications. Disease incidence (DI) and disease severity (DS) were rated on a pot basis at 21 and 28 days post-inoculation. A disease index (DX) was calculated as ((DI x DS)/11). The 95% confidence intervals (CI) for DX of the parents were calculated and used to classify the F2:3 lines as susceptible, resistant or segregating. Segregating lines were scored on an individual plant basis. Chi-square tests determined the goodness-of-fit of observed F2:3 segregation data to the expected genetic ratios. Hartwig (SDS-R) and Camp (SDS-S) consistently had low and high DX ratings, respectively. The DX of SDS foliar ratings of the F2:3 lines segregated in a 7:8:1 (resistant:segregating:susceptible) ratio (χ2 = 4.514, P = 0.1046), indicating that Hartwig carries two dominant genes conferring resistance to SDS and can serve as a good source of resistance for crossing in a breeding program.