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[Tumor necrosis factor gene expression in cervical carcinoma by in situ hybridization].

TNF mRNA expression in cervical carcinoma was observed by the use of 3H and digoxigenin labelled TNF-alpha cDNA probes and in situ hybridization technique. Of 33 cancer samples tested, 30 were seen to have positive TNF mRNA expression in cancer cells and in some tumor infiltrating lymphocytes, the average positive rate being 12.18% and 3.63% respectively with a positive linear correlation between them. The poorer the cell differentiation, the more TNF mRNA expression in the cancer cells. The present findings suggest that TNF in cancer cells may possibly be a marker for tumor staging.