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Use of Microdialysis for In‐vivo Monitoring of Hydroxyl Free‐radical Generation in the Rat

Free‐radicals are reported to cause the tissue‐damage associated with some toxins and diseases, yet there is no suitable method for routine in‐vivo monitoring of these species. This paper introduces an in‐vivo microdialysis technique in which the hydroxyl radical reacts with salicylate to generate dihydroxybenzoic acids (DHBA) which are measured by HPLC with electrochemical detection.