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Core–Shell HA-AuNPs@SiNPs Nanoprobe for Sensitive Fluorescence Hyaluronidase Detection and Cell Imaging

A highly sensitive and selective fluorescence strategy for hyaluronidase assay was developed by coupling silicon nanoparticles (SiNPs) as fluorescence indicators with hyaluronic-acid-functionalized gold nanoparticles (HA-AuNPs) as quenchers. With the formation of a core–shell HA-AuNPs@SiNPs nanoprobe by self-assembly, the fluorescence of SiNPs could be quenched by HA-AuNPs through fluorescence resonance energy transfer (FRET). The addition of hyaluronidase leads to cleavage of HA into small fragments, and SiNPs are released. As a result, an immediately fluorescent recovery appears when HAase is introduced. The proposed fluorescence method showed a linear response ranged for HAase from 0.01 to 10 U/mL, and a detection limit of 0.004 U/mL (S/N = 3) was obtained. Furthermore, it can be employed as an enzyme activatable nanoprobe for cell imaging applied in diagnostic and related biological studies.