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Expression and Distribution of Kinin B2 Receptor in Medulla and Spinal Cord Tissues of Rats Treated with Capsaicin

Kinins are vasoactive peptides that promote pain and inflammation, yet centrally believed to participate to cardiovascular defensive reflexes produced by noxious stimuli. These peptides signal through the activation of two transmembran e Gprotein-coupled receptors named B 1 and B 2 receptors (B 1 R and B 2 R). The B 2 R is constitutive in healthy tissues and animals. The aim of the study was to measure the gene and protein expression of B 2 R kinin receptors in central and peripheral tissues isolated from control rats and rats were pre-treated with capsaicin on the second day of life (50 mg/kg, s.c.) or two weeks prior to sacrifice (125 m g/kg over three days, s.c.). The same treatment with saline was made in control animals. Levels of mRNA for B 2 R were measured by quantitative RT-PCR and Qualitative while receptor binding sites were measured on tissue sections with the radioligands I-HPP-Hoe 140 (B 2 R). B 2 R was expressed in all studied tissues (hypothalamus, paratrigeminal nucleus, nucleus of solitary tract, spinal cord, aorta and liver) and treatment capsaicin neonates when compared to controls, did not affect its level of expression. Capsaicin had no significant ef fect on the expression of B 2 R in some tissues on binding sites. The synthesis of B2R kinin receptor is not associated with sensory C-fibre and tissues showed no significant difference indicating that B 2 R was regulated by distinct mechanisms.