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A Gambogic Acid-Loaded Delivery System Mediated by Ultrasound-Targeted Microbubble Destruction: A Promising Therapy Method for Malignant Cerebral Glioma

Background The blood–brain barrier (BBB) inhibits the delivery of macromolecular chemotherapeutic drugs to brain tumors, leading to low utilization rates and toxic side effects to surrounding tissues and organs. Ultrasonic targeted microbubble destruction (UTMD) technology can open the BBB, leading to a new type of drug delivery system with particular utility in glioma. Purpose We have developed a new type of drug-loaded microbubble complex based on poly(lactic-co-glycolic acid) (PLGA) that targets gambogic acid (GA) to the area of brain tumors through UTMD. Methods GA/PLGA nanoparticles were prepared by the double emulsification method, and cationic microbubbles (CMBs) were prepared by a thin film hydration method. The GA/PLGA-CMB microbubble complex was assembled through electrostatic attractions and was characterized chemically. The anti-glioblastoma effect of GA/PLGA-CMB combined with focused ultrasound (FUS) was evaluated by biochemical and imaging assays in cultured cells and model mice. Results GA/PLGA-CMB combined with FUS demonstrated a significant inhibitory effect on glioblastoma cell lines U87 and U251 as compared with controls (P<0.05). Tumor access and imaging analyses demonstrated that administration of GA/PLGA-CMBs combined with FUS can open the BBB and target the treatment of glioblastoma in a mouse model, as compared with control groups (P<0.05). Conclusion The combination of PLGA-CMB with FUS provides an effective and biocompatible drug delivery system, and its application to the delivery of GA in a mouse glioblastoma model was successful.


Synthesis of PLGA-CMBs
PLGA and DSPC were dissolved at a mass ratio of 20:1 in 1 to 3 mL dichloromethane. The following ultrasonic parameters were used: power 900 W, power ratio 60%, ultrasonic on time 3 s and off time 3 s, and working cycle 2 min. After 5 mL 4% polyvinyl alcohol was added, the suspension was homogenized for 5 min. Double distilled water (10 mL) was added to the homogenized liquid, and the mixture was stirred for 2 to 3 h. After centrifugation at 4500 rpm for 3 min, the supernatant was discarded, 1 mL double distilled water was added, and the sample was incubated overnight at −80°C. After freeze-drying for 48 h, PLGA nanobubbles (NBs) were obtained and stored at −20°C.
A cationic microbubble solution was prepared with DSPC, PEG-2000, PEI and stearic acid as the main components. After air was removed with a vacuum, the cationic microbubble was filled with inert gas (C 3 F 8 ), and the cationic microbubble (CMB) suspension was obtained after shaking for 45s. Aliquots of 100 μL CMB suspension were mixed with 80 μg PLGA NBs, resulting in a PLGA-CMB suspension. Defined amounts of GA were added to the PLGA-CMB raw material to obtain CA/PLGA-CMBs.

Characterization of GA/PLGA-CMBs
The drug loading and entrapment efficiency were calculated by spectrophotometry. The drug loading and entrapment efficiency of GA/PLGA dissolved in DMSO were then calculated. Drug loading was calculated as LE(%) = W e /W m × 100%, where LE represents drug loading, W e represents the amount of drug encapsulated in the lipid and W m represents the total weight of GA/PLGA NBs. Entrapment efficiency was calculated as EN% = (1 -C f /C t ) × 100, where EN represents the drug entrapment efficiency as a percentage, C f is the amount of free drug and C t is the total amount of drug (free and entrapped). The particle size and potential distribution of GA/PLGA-CMBs were detected by a Malvern particle size analyzer, scanning electron microscopy and transmission electron microscopy.

Physiological Stability of GA/PLGA-CMBs
The GA/PLGA-CMBs showed a good physiological stability. B-mode scan ultrasonography was performed using a Sonix SP High Performance B-mode System (Ultrasonix, Richmond, BC, Canada). The age of BALB/c mice is 6-7 weeks. Each mouse was injected with 100 μL GA/PLGA-CMBs suspension via tail vein. Ultrasound imaging experiments were carried out at 0-40 min. The liver US image in comparative mode was recorded. Further analysis was performed using a high frequency US scanning high-resolution B-mode ultrasonography system. High-resolution B-mode ultrasonography of the right liver was performed with a linear 8 MHz frequency.

Cell Culture
Dulbecco's modified Eagle's medium containing 10% fetal bovine serum and 1% penicillin and streptomycin was used for culturing U87 and U251 cells. Cells were cultured in an incubator at 37°C and 5% CO 2 . Cells were passaged with 0.25% trypsin when confluence reached 80 to 90% at the logarithmic growth phase and were diluted to 1×10 5 cells/mL. Cells were cryopreserved in a solution containing 10% DMSO. Drugs were dissolved in a solvent containing phosphatebuffered saline (PBS) with DMSO (5%) and Tween-80. The concentration of drug was adjusted so that animals were administered 1.5 μmol/L.

Measurements of Drug-Release Behavior
Aliquots (100 μL) of a suspension of GA/PLGA-CMBs were placed in wells of a 96-well plate. The samples were subjected to ultrasound under the following conditions: frequency 949 KHz, 10,000 cycles, 5% duty cycle, 1 s pulse time and 60s action time. The sound pressure intensity was increased from 0 to 0.88 MPa, and the concentration of GA/PLGA-CMBs in suspension at each sound pressure intensity interval was detected by enzyme labeling and spectrophotometry at λ = 500 nm.
Using the same ultrasonic parameters as above, the viability of U251 cells treated with different sound pressure intensities was detected via CCK-8 assays. Then, U251 cells were incubated with different concentrations of GA for 24 hours, and the cell viability of U251 cells in each group was detected via CCK-8 assays. The GA concentrations of all treatment groups in Figures 2-4 were consistent. The concentration was 1.5 μmol/L.

CCK-8 Assays for Cell Viability
Wells of a 96-well plate were seeded with 100 μL of a 5×10 4 cells/mL suspension, and the plates were then incubated at 37°C and 5% CO 2 for 24 h in serum-free medium. Then, 10 μL of CCK-8 solution was added to each well. The absorbance at 450 nm was

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A s was the absorbance of the experimental well including medium, CCK-8 reagent and GA, A c was the absorbance of the control well without GA and A b was the absorbance of the blank well without cells or CCK-8 reagent.

Detection of Apoptosis
In order to detect apoptosis by flow cytometry, cells growing in suspension were collected by centrifugation at 500g for 5 min, and adherent cells were collected by trypsin digestion without EDTA. Cells were washed with 10 mL PBS and collected by scraping in 5 mL PBS followed by centrifugation at 2000 RPM for 5 min at 4°C. Then, 300 μL of binding buffer and 195 μL Annexin V-FITC binding buffer were added, followed by incubation with 5 μL annexin V-FITC and 10 μL propidium iodide Apoptosis was also quantified using a microscopy-based kit. Double staining of U87 cells with AlexaFluor 488annexin V and PI enabled the discrimination of live (AlexaFluor 488-/PI-), early apoptotic (AlexaFluor 488+/PI-), late apoptotic (AlexaFluor 488+/PI+), or necrotic (Alexa Fluor 488-/PI+) cells. Cell nuclei were counterstained with DAPI, and fluorescence images were acquired using a fluorescence microscope (DMI3000B, Leica, Germany). Fluorescent signal was quantified by ImageJ software.

Animals and Tumor Models
Female nude BALB/c mice, aged 6 to 8 weeks, were purchased from Beijing HFK Bioscience (Beijing). They were raised in a specific pathogen-free (SPF) animal room at a constant temperature (25°C). All animal procedures were reviewed and approved by the Institutional Animal Care and Use Committee at Xinxiang Medical University and are in  Mice were anesthetized with 4% isoflurane/O 2 gas, and anesthesia was maintained with 1.5% isoflurane/O 2 gas. U87-Luc cells carrying luciferase (Luc) gene fragments were implanted in order to generate the mouse glioma model. According to the mouse brain atlas, the anterior fontanelle was coordinate zero, and 2.5 μL of a U87-Luc cell suspension in PBS with a cell density of 2×10 5 cells/mL was injected at the coordinate (1.0 mm, 1.5 mm, 2.0 mm). The injection time was 5 min, and the needle was retained for 10 min. Penicillin (10,000 U/day) was injected intramuscularly every day for 1 week following surgery.

Construction of the in vitro BBB Model
Cells of the mouse brain microvascular endothelial cell line bEend3 were inoculated on a Transwell membrane. After 5 days of routine culture, the in vitro BBB model was established. High glucose medium (500 μL) was added to the upper and lower chambers of the Transwell apparatus, and 500 μL of NaF (10 µg/mL) was added to the upper chamber.
The BBB model was treated with 5 different treatments: untreated (control), ultrasound-only (FUS), empty phospholipid microbubbles (MB), ultrasound combined with empty phospholipid microbubbles (FUS+MB) and ultrasound combined with drug-loaded microbubble complex (FUS+GA/PLGA-CMB). The system was treated with ultrasound following the parameters noted above, and the sound pressure intensity was 0.63 MPa. Cells were stained with an anti-Zo-1 antibody and a secondary antibody that was indicated by green fluorescence, and DAPI-labeled nuclei were blue as observed by fluorescence microscopy. The fluorescence signal value of NaF in the lower chamber was detected by an enzyme labeling instrument.

In vivo Assays of BBB Opening
The experiment was divided into three groups: a group treated only with ultrasound (FUS), a group treated with ultrasound and GA/PLGA microbubbles (FUS+GA/PLGA) and a group treated with ultrasound combined with the drug-loaded microbubble complex (FUS+GA/PLGA-CMB). For treatment, 50 μL of a GA/PLGA or GA/PLGA-CMB suspension was injected into the tail vein of each mouse. After 1 min, the sound pressure intensity was adjusted to 0.50, 0.63, 0.76, 0.88 or 1.01 MPa for 30s. After the treatment of FUS combined with GA/PLGA-CMBs, the corresponding brain tissue was perfused and processed under different sound pressure intensities, embedded in paraffin, stained with hematoxylin and eosin, and stained with DAPI (nuclei) and TUNEL (apoptotic cells). Hematoxylin and eosin (H&E) staining was performed according to a standard H&E protocol. The apoptosis rate, as determined by TUNEL labeling, was assessed with ImageJ software.

In vivo Imaging
Anesthesia of mice was induced with continuous application of 1.5% isoflurane. Each mouse was injected intraperitoneally with 100 μL of a solution of D-fluorescein potassium salt (Alameda, CA, USA; 15 mg/mL in normal saline). After 15 min, the fluorescence signal was detected with a live animal imaging system (IVIS) (Lumina LT; PerkinElmer, Inc.).

Evaluation of Anti-Tumor Effects
Brain glioma model mice were treated on the 10th day after successful establishment of the glioma model. The treatment occurred once every 2 days for a total of 3 treatments. The mice in each group were imaged by IVIS on days 10, 17 and 24 after successful establishment of the glioma model. On day 17, the brains were perfused, and the tumor tissues were sectioned in paraffin. An anti-PCNA antibody and an anti-Ki-67 antibody were used to detect tumor proliferation. TUNEL assays were performed using a TUNEL Apoptosis Detection Kit (Keygentech, China) for the detection of apoptotic tumor cells in the paraffin-embedded tumor tissue. At day 37 following establishment of the model, the brains were perfused to observe the appearance of the tumor tissue, and the tumor volumes were measured. The number of mice surviving in each group on day 60 was recorded.

Statistical Analysis
Data were analyzed using SPSS 22.0 statistical software and presented as the mean ± SD. The one-way ANOVA test was applied for evaluating possible statistical differences among three or more groups of data. P < 0.05 was considered statistically significant (In each figure, statistical significance is indicated with the following symbols: *P < 0.05, **P < 0.01, ***P < 0.001, #P < 0.05, ##P < 0.01, ###P < 0.001, nsP > 0.05).

Synthesis and Characterization of GA/PLGA-CMB
The results of scanning electron microscopy (SEM) and tunneling electron microscopy (TEM) analyses showed that the GA/ PLGA was spherical, the surface contained both drug molecules and pores, and the particle sizes were dispersed uniformly ( Figure 5A and B). CMBs were manifest as spherical and hollow structures under TEM ( Figure 5C). The GA/PLGA-CMB complexes were confirmed to be composed of both CMBs and GA/PLGA ( Figure 5D) according to TEM images.
The entrapment efficiency and drug loading was quantified by adding varying amounts of GA nanoparticles to a constant mass (50 mg) of PLGA. Here, the entrapment efficiency and drug loading of GA/PLGA prepared by adding GA in a ratio of 4 mg GA to 50 mg PLGA were 83.04 ± 7.51% and 6.64 ± 0.60%, respectively ( Figure 5E and F). Malvern particle size analyses showed that the average particle size distribution of the GA/PLGA-CMBs was 951.37 ± 110.32 nm,

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International Journal of Nanomedicine 2022:17 and the potential distribution was 5.30 ± 0.49 mV (Figure 5G and H). The distribution was shown to be normal. The results of physiological stability test showed that GA/PLGA-CMBs could persist around 30 min in vivo ( Figure 6).

Drug Loading and Release
To measure the ability of the particles to release drug upon application of ultrasound, FUS was applied under conditions noted in Materials and Methods, and the quantity of drug released was determined by measuring the spectroscopic absorbance of the supernatant at 500 nm. The concentration of drug released upon application of a sound pressure of 0.63 MPa (OD 500 = 1.07 ± 0.07) was significantly different from that when the sound pressure was zero (OD 500 = 1.51 ± 0.03) (P < 0.01, Figure 2A).
The biological effects of this sound application were also tested. When the sound pressure intensity was 0.63 MPa, FUS had no effect on the viability of U251 cells, as detected by CCK-8 assays. The U251 cell viability was 68.17 ± 7.70% when the sound pressure intensity was 0.76 MPa, and the U251 cell viability was 54.59 ± 4.01% when the sound pressure intensity was 0.88 Mpa. The difference in cell viability following treatment with 0.76 MPa was significantly higher than that following treatment with 0.88 MPa FUS (P < 0.01, Figure 2B).
After U251 cells were incubated with free GA at 1.5 μmol/L for 24 h, the cell viability was 48.71 ± 2.84%, which was significantly lower than the viability of cells treated with vehicle for 24 h, which was defined as 100% (p < 0.05, Figure 2C).

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Application of FUS combined with GA/PLGA-CMBs was found to open the model BBB (see, especially, the white arrow in Figure 2D). After opening the model BBB, NaF was able to infiltrate from the upper chamber of the model apparatus into the liquid in the lower chamber. The opening of the BBB was demonstrated by an increase of the NaF signal to 1.30 ± 0.04% in the lower chamber solution, which was significantly higher than the signal in the control system (p < 0.001, Figure 2D and E).

Inhibition of Growth of Brain Glioma Cell Lines
U251 cells were treated with FUS and GA/PLGA-CMBs for 24 h. The final drug concentration was 1.5 µmol/L relative to GA, the sound pressure intensity was 0.63 MPa, the action time was 30s, and other FUS parameters were as noted in Materials and Methods. Following the treatment, the rate of apoptosis was detected by flow cytometry. The rate of apoptosis of cells treated with FUS and GA/PLGA was 76.73 ± 2.70%, and the rate of apoptosis of cells treated with FUS and GA/PLGA-CMB was 83.43 ± 1.43%. As shown in Figure 3A and C, the difference in the rate of apoptosis between these two treatment groups was significant (p < 0.001).
The viability of U251 cells post-treatment was detected by CCK-8 ( Figure 3B). The viability of cells treated with FUS and GA/PLGA-CMB was 0.81 ± 0.26%, which was significantly different from the viability of control cells 100 ± 13.62% (p < 0.001).
We performed the same test with another GBM cell line, U87, and the results were similar to those found for U251. Here, the rate of apoptosis of U87 cells treated with FUS and GA/PLGA-CMB (27.68 ± 0.89%) was significantly higher than that of apoptosis of cells treated with FUS and GA/PLGA (18.9 ± 0.78%; p < 0.01; Figure 4A and C). The viability of U87 cells treated with FUS and GA/PLGA-CMB was 49.34 ± 1.95% and was significantly different from that of the control group (p < 0.01; Figure 4B).
After U87 cells were cultured for 24 h under various treatments, rates of apoptosis were detected using a YF488-Annexin V/PI apoptosis detection kit ( Figure 7A). Early apoptotic cells were labeled with YF488, and late apoptotic cells were labeled with PI. The nuclei of cells in each group were labeled with DAPI, and the cells in each group were counted using ImageJ. The results showed that compared with other groups, the number of U87 cells in the group treated with FUS and GA/PLGA-CMB was the lowest (P < 0.001; Figure 7B). The results also demonstrated that apoptosis and necrosis occurred in all of the groups of U87 cells. The apoptotic rate of U87 cells treated with FUS and GA/PLGA-CMB was the highest, and it was significantly higher than that of other groups (P < 0.001; Figure 7C). In addition, this experiment demonstrated that FUS combined with GA/PLGA-CMB induced the apoptosis of U87 cells only under certain ultrasound parameters (Figure 7). These results suggest that the sound pore effect mediated by GA/PLGA-CMB under the action of FUS increased the permeability of the U87 cell membrane, promoted the entry of GA/PLGA into U87 cells, stimulated the release of GA, and increased the apoptotic rate of U87 cells.

Opening of the BBB in vivo
We used a mouse model to further explore the opening of the BBB in vivo. The permeability of the blood-brain barrier was evaluated using Evans blue dye (EB, Sigma Co. USA). The results of permeability studies show that FUS combined with GA/PLGA-CMBs enhanced the opening of the BBB when the ultrasonic intensity was between 0.63 and 1.01 MPa. FUS alone or FUS with GA/PLGA did not open the BBB (Figure 8). The brain tissues of mice treated with different ultrasound intensities were embedded in paraffin and stained with H&E ( Figure 9A). Microscopic analyses of these tissues showed that when the ultrasound intensity was between 0.69 and 0.88 MPa, the erythrocyte extravasation became obvious ( Figure 9B). The relative amount of erythrocyte exudation (86.67 ± 9.53) was significantly higher than that in tissues treated with an ultrasound intensity of zero MPa (7 ± 1.63; p < 0.05).
We also labeled apoptotic cells using a TUNEL kit and labeled nuclei with DAPI. Quantification of the rate of apoptosis demonstrated that the rate of apoptosis was relatively high when the ultrasound intensity was between 0.69 and 0.88 MPa. When the ultrasound intensity was 0.88 MPa, the rate of apoptosis was 79.03 ± 5.12% and was significantly higher than that in tissues treated with an ultrasound intensity of zero MPa (16.03 ± 2.41%; p < 0.05) ( Figure 9C).

Evaluation of the Anti-Tumor Effect
IVIS was used to detect the growth of tumors in mice treated under various conditions. The treatment began on the day 10 after the establishment of the model and was performed once every 2 days, for a total of 3 treatments. The luciferase Tumor proliferation and apoptosis were detected by immunohistochemistry. The positive expression of markers of proliferation and apoptosis was observed by microscopy ( Figure 11A). The tumor proliferation rate of each group was detected with immunohistochemistry utilizing an anti-PCNA antibody, and the tumor proliferation rate in mice treated with FUS and GA/PLGA-CMB was 31.77 ± 3.57% (p < 0.001 vs control group; Figure 11B). In addition, the tumor proliferation rate of each group was detected by an anti-Ki-67 antibody, and the tumor tissue growth rate of mice treated with FUS and GA/ PLGA-CMB was 25.94 ± 3.12% (p<0.001 vs control group; Figure 11C). A TUNEL antibody was used to detect apoptosis within the tumors of each group of similarly treated mice. The results showed that the rate of apoptosis of tumor tissue in mice treated with FUS and GA/PLGA-CMB group was 78.98 ± 7.15% (p<0.001 vs control group; Figure 11D).
Mice were also subjected to general tests for anti-tumor viability following the treatment. On the 37th day after the establishment of the model, the brains of the mice in each treatment group were perfused for observation ( Figure 12A). The volume of tumors from mice treated with FUS and GA/PLGA was 0.17 ± 0.04 mm 3 , while the volume of tumors from mice treated with FUS and GA/PLGA-CMB was 0.04 ± 0.01 mm 3 (p<0.001 vs control group; Figure 12B). This reduction in tumor size correlated with life span, as the longest lived mice were those treated with FUS and GA/PLGA-CMB group, which lived 45±11 d (p<0.05 versus GA/PLGA group; Figure 12C).

Discussion
Delivering chemotherapeutic drugs into brain tumors remains an important challenge. 30 The existence of biological barriers such as the BBB and BTB limits the penetration of most chemotherapeutic agents. 8 In addition, the complexity 2012 of brain tumors and the lack of effective brain drug delivery techniques have had a significant impact on the survival of patients. Among different types of brain tumors, GBM is a highly invasive, aggressive and malignant tumor. New treatments for this important disease are needed. Recent studies have shown that ultrasound targeted microbubble destruction (UTMD) can safely and effectively open the BBB and BTB and deliver drugs to brain tissue, which provides a new strategy for the treatment of brain diseases. 19,23,24,31,32 Because of the potential advantages of the UTMD system, we developed a new drug-loaded microbubble complex (PLGA-CMB) using DSPC, PEG-2000, PEI and PLGA as raw materials. PLGA-CMB was found to be stable, to have a high drug loading content, and to provide a long imaging duration in vivo. To take advantage of these properties, we prepared PLGA-CMB complexes containing GA (Figure 1).
PLGA-CMB complexes consist of drug-loaded PLGA nano-microbubbles and phospholipid cationic microbubbles, and in particular, our previous studies have shown that drug-loaded PLGA nano-microbubbles have good stability. 33 In addition, the core of phospholipid cationic microbubbles is an inert gas (C 3 F 8 ) that has been shown to be relatively stable under physiological condition. [34][35][36][37] The results of Omata et al, who found that perfluoropropane and perfluorobutane-loaded MBs (MB-C 3 F 8 and MB-C 4 F 10 ) showed sustained ultrasound imaging stability in vitro and in vivo compared with sulfur hexafluoride-loaded MBs (MB-SF 6 ), 37 suggested that perfluoropropane and perfluorobutane could be useful for the production of MBs with high stability to allow for US imaging and drug delivery. 37 In this study, the results shows that PLGA-CMB complexes, with C 3 F 8 core, have optimal stability under physiological conditions ( Figure 6).
Our previous studies have shown that porous lipid GA/PLGA microbubbles combined with FUS have led to an opening of an in vitro BBB model. 33 However, we found that the opening effect of porous lipid GA/PLGA microbubbles combined with FUS on the BBB in vivo is not ideal. Therefore, here, we developed a new type of GA/PLGA-CMBs complex that incorporates microbubble technology. Compared with GA/PLGA, GA/PLGA-CMBs have a more obvious opening effect on the BBB in vivo under the action of FUS.
In this study, PLGA nanoparticles containing GA were prepared by the double emulsion method. 38,39 Negatively charged GA/PLGA nanoparticles were connected to the surface of positively charged cationic microbubbles (CMBs) to form GA/PLGA-CMB microbubble complexes. 34,40 We demonstrate that GA/PLGA-CMBs can produce a sound pore effect under the action of FUS, which leads to the increase of cell membrane permeability. 41 In in vitro experiments, the cavitation effect supported by GA/PLGA-CMB under the action of low frequency FUS induced permeability of the cell membrane, which further improved the killing effect of GA on two GBM cell lines, U87 and U251. 42,43 When a suspension containing GA/PLGA-CMB was injected into the caudal vein of mice, it ultimately reached the area of the induced brain tumor via the circulation. Under the action of FUS with specific sound pressure intensities, steady-state cavitation occurred in the GA/PLGA-CMB complexes, resulting in an increase in the permeability of the BBB and BBTB. GA/PLGA-CMB was uniformly distributed in the tumor tissue area, which resulted in increased sound Notably, these results suggest that these efforts may be expanded to the visual targeting of anti-tumor therapies. Visual targeting makes use of ultrasonic imaging, which is a real-time imaging technology that can visualize the structures of organs. [44][45][46] MBs in particular can be used as contrast agents to enhance the utility of ultrasound images. 45 By choosing the appropriate ultrasound frequency and other ultrasound parameters, we predict that it will be possible to realize tumor imaging and tracking of the drug release process of GA/PLGA-CMBs under the action of FUS. In addition, this technique will allow the detection of the viability of microbubbles in vivo through passive cavitation detection, which has been tested widely in clinical trials. 28,47,48 The ultrasonic parameters such as sound pressure intensity, duty cycle, action time, cycle time and pulse interval have obvious influences on the effects of FUS. Our results show that when other parameters are constant and microbubbles are combined with ultrasound, as the sound pressure intensity increases, the cavitation effect becomes more obvious, and the permeability of the physiological barrier becomes higher. For our next step, then, we plan to use this method to carry out in vivo experimental research to explore the safety range of FUS parameters. Whether the shell will cause inflammation after the rupture of microbubbles will also be a focus of attention. The biosafety of microbubbles is also of great concern, and we plan to systematically evaluate the biosafety of GA/PLGA-CMB. 28,49,50

Conclusion
We have developed a new type of drug-loaded microbubble complex (PLGA-CMB). The microbubble complex can carry drugs with high volume. PLGA-CMBs can produce a cavitation effect under the action of UTMD and can thus open up the BBB and BBTB to efficiently target brain tumors. This microbubble complex provides a new strategy for the ultrasound-targeted treatment of tumors. Effective on-demand drug delivery can be achieved by using PLGA-CMBs in a sonodynamic-dependent manner both in vitro and in vivo. The explosion of PLGA-CMBs under the action of FUS provides a promising platform for the delivery of anti-tumor therapeutic agents.