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A blastogenic test for foot-and-mouth disease

A blastogenic test to detect peripheral blood leukocytes specifically sensitized to foot-and-mouth disease virus antigen is described. The test is carried out in microtitre plates and optimum conditions were found by titration. These employed 7.5 × 105 cells/well and 20 complement fixing units of antigen. Peak [3H]thymidine incorporation was found to take place at 2–3 days.