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Cell‐permeable organic fluorescent probes for live‐cell super‐resolution imaging of actin filaments

The recently developed super-resolution microscopy has been applied to characterize the nanometer-scale structure of actin filaments in live cells, owing to its live-cell imaging ability at sub-diffraction resolution. However, there are still some limitations in existing live-cell labeling methods for super-resolution imaging of F-actin. A series of cell-permeable fluorescent probes was developed for super-resolution imaging of actin filaments with the introduction of a cell-penetrating peptide (rR)3R2. These probes are highly specific, multicolor available, and suitable for different cell lines. With these probes, we resolve actin filaments at ~60-nm spatial resolution in live-cell super-resolution imaging. Our results here extensively expand the types of probes for live-cell super-resolution imaging of F-actin and open up a new avenue in the design of cell-permeable fluorescent probes. REFERENCE [1] Y. Han, M. Li, M. Zhang, X. Huang, L. Chen, X. Hao, C. Kuang, X. Liu, and Y.-H. Zhang, “Cell-permeable organic fluorescent probes for live-cell super-resolution imaging of actin filaments,” J. Chem. Technol. Biotechnol., 94, 2040-2046 (2019).