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In vitro healing efficacy of stem cell secretome and cord blood platelet lysate on a chronic wound model

Purpose: A chronic wound model provides an opportunity to understand fundamental mechanisms that could possibly provide leads for novel diagnostic molecules. An in vitro chronic wound model was created to evaluate the healing potential of stem cell (SC) secretome obtained from human umbilical cord-derived mesenchymal stem cells (hUCMSCs) and cord blood platelet lysate (CBPL).
Methods: hUCMSCs were obtained from explant culture of umbilical cord. SC secretome was collected from cells at passage 2 after appropriate SC characterisation. Platelets were isolated from cord blood and exposed to a repeated freeze–thaw cycle to obtain CBPL. An in vitro chronic wound model was created using dimethyl sulfoxide (DMSO) and hypoxia in HEK293 cells through scratch in a cell monolayer. Wounds were exposed to SC secretome and CBPL and their wound closure efficacy was calculated as relative wound density (RWD%) at defined time points.
Results: MSCs with typical spindle-shaped morphology were isolated from explant culture that sustained their stemness and morphology until up to passage 10. DMSO efficiently impeded the movement of cells into the wound area to generate a chronic wound model which was efficiently removed upon addition of SC secretome and CBPL.
Conclusion: A transient in vitro chronic wound model was created successfully to determine the healing efficiency of SC secretome and CBPL.