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Determining the Best Pheromone-Baited Traps for Capturing Scyphophorus acupunctatus (Coleoptera: Dryophthoridae) in Mezcal Agave

Summary Three traps (TOCCI, TOCCIA, and container) lured with synthetic aggregation insect pheromone and food attractant, were evaluated in the capture of Scyphophorus acupunctatus Gyllenhal (Coleoptera: Dryophthoridae) under field conditions on mezcal agave in Guerrero, Mexico. TOCCI and TOCCIA traps were the most efficient for capturing migrant adults. Most of the captured specimens were females, so these traps can reduce future populations of this insect.

Sampling agave fields is complicated because leaves are succulent and rigid and end in a dangerous sharp terminal spine. Thus, the direct sampling method requires dissection of agave crowns (Solís-Aguilar et al. 2001), but it increases operating costs, as it is time consuming. On the other hand, the indirect method is by monitoring the immigrating weevil adults with a pheromone-based trapping system developed in blue agave (Agave tequilana Weber 'Azul'; Asparagaceae) (Ruiz- Montiel et al. 2003Montiel et al. , 2008Rodríguez-Rebollar et al. 2012). Until now, 3 types of promising pheromone and food attractant based traps have been tested for monitoring agave weevil in Mexico; on Jalisco State's blue agave, the TOCCI and TOCCIA traps were evaluated by Bravo-Pérez (2009) andFigueroa-Castro (2014). A 20 L container trap was tested by Azuara-Domínguez et al. (2014), and a 5 L container trap was tested on P. tuberosa 'Perla' (Asparagaceae) by López-Martínez et al. (2011) and García-Ramírez et al. (2014).
Recently, the agave industry in Guerrero has increased to 1,814.41 ha with agaves "papalote" and "espadín" (SIAP 2016) dedicated to low technology mezcal production. Local farmers have requested more efficient traps to monitor the agave weevil under local environmental conditions. Here, we evaluated the 3 main types of pheromone and food baited traps used previously in Mexico for capturing immigrating S. acupunctatus adults in mezcal agave fields.
We performed 2 experiments in a 4-yr-old "maguey espadín" plantation at "El Rancho Frio" (18.386778°N, 99.166111°W; 1,120 m asl); this plantation is located at Quetzalapa, Guerrero, Mexico. The 1st experiment was performed from 16 Sep to 27 Nov 2015, and the 2nd experiment was performed during 2 mo from 27 Nov 2015 to 29 Jan 2016.
In the 1st experiment, we evaluated 3 types of traps to capture the agave weevil: 1) TOCCI trap (  García-Ramírez et al. (2014). A completely randomized experimental design was used, and each trap was replicated 5 times. The distance between traps was 50 m, and traps were placed in a square distribution pattern as recommended by Figueroa-Castro et al. (2016). Traps were checked 5 times, i.e., every 15 d from 16 Sep to 27 Nov 2015.
In the 2nd experiment, the 2 traps with the best performance in experiment 1 were compared: TOCCI trap, and TOCCIA trap (buried at the level of "cones" for weevils entry). The experiment was established in a completely randomized design, and each treatment was replicated 6 times. The distance between traps was 50 m, and all traps were distributed in a square pattern as indicated previously. Traps were checked 4 times, every 15 d, from 27 Nov 2015 to 29 Jan 2016. In both experiments, on each date, we emptied the traps and collected the captured weevils.
The response variable was the average number of captured agave weevils per trap per evaluation date, and within each experiment. Captured weevils were taken to the laboratory for counting and sexing with a stereoscopic microscope (Motic® SMZ-168, British Columbia, Canada) (Ramírez-Choza 1993).
Each trap was baited with an S. acupunctatus synthetic aggregation pheromone dispenser (FeroComps, Mexico City, Mexico) plus 300 g of recently cut agave tissue (Figueroa-Castro et al. unpublished). The agave tissue was placed in a 0.5 L plastic container with 8 holes (0.5 cm diameter each), and sprayed with malathion (Malathion 1000®, Anajalsa, S. A. de C. V., Jalisco, Mexico) at doses of 10 mL/L of water. The pheromone dispenser was changed on a monthly basis, and the agave tissue was replaced biweekly.
All statistical analyses were performed using SAS statistical software (SAS Institute 2004). The data were analyzed by analysis of variance (ANOVA) using PROC GLM. Prior to ANOVA, data normality (Shapiro-Wilk test) and homogeneity of variances (Bartlett test) were tested and data transformation was not necessary for the 1st experiment. Treatment means were separated with a Tukey test (a = 0.05). For the 2nd experiment, data were transformed by √(x+0.5) and a t-test was performed. A chi-squared test was performed to determine differences in the number of females and males captured in both experiments.
The low efficacy of container traps could be a result of a natural variation in weevil populations between the dates of the 1st and 2nd experiment (345 and 155 specimens collected in 1st and 2nd experiments, respectively). Apparently, container trap design could play a role in capturing agave weevils at high densities. In other crop species, López-Martínez et al. (2011) and García-Ramírez et al. (2014) deployed these traps for S. acupunctatus on P. tuberosa and captured up to 700 weevils per trap per wk. This aspect has not been adequately studied. Furthermore, we detected important disadvantages for container trap design in agave "espadín" as follows: many non-target Lepidoptera, Diptera, and Coleoptera species were collected; soapy water used in traps was an alternative source for wild fauna's hydration, affecting its efficacy; and some rodents consumed the captured weevils affecting the results.
In addition, we found that in the 1st experiment more females than males were captured (Fig. 2) in TOCCI traps (χ 2 = 22.28, P = 0.0001), in TOCCIA traps (χ 2 = 5.38, P = 0.02), and in container traps (χ 2 = 17.73, P = 0.0001). Also, in the 2nd experiment more females than males were captured (Table 1) in TOCCI traps (χ 2 = 20.25, P = 0.0001) and in TOCCIA traps (χ 2 = 29.39, P = 0.0001). Differences between captures of females and males in pheromone-baited traps were reported also by Figueroa-Castro et al. (2013) on blue agave in Jalisco, and by Macedonio- Guevara (2015) in mezcal agave in Guerrero. Capturing large numbers of females has an important impact on future population densities of agave weevil, with the potential of reducing egg laying in agave crops.
We thank Daniel Castro Rodríguez, Timoteo Castro Rodríguez, and Luis Figueroa Ocampo for their assistance in the establishment and evaluation of experiments on their agave fields. Also, we thank Programa para el Desarrollo Profesional Docente (SEP PRODEP) for providing a Postdoctoral scholarship for the first author at the Facultad de Ciencias Agropecuarias at the Universidad Autónoma del Estado de Morelos. Comments from 2 reviewers improved the manuscript.

Summary
Three traps (TOCCI, TOCCIA, and container) lured with synthetic aggregation insect pheromone and food attractant, were evaluated in the capture of Scyphophorus acupunctatus Gyllenhal (Coleoptera: Dryophthoridae) under field conditions on mezcal agave in Guerrero, Mexico. TOCCI and TOCCIA traps were the most efficient for capturing migrant adults. Most of the captured specimens were females, so these traps can reduce future populations of this insect.