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Simultaneous Monitoring of Target Compounds and Carbohydrate Patterns During Pharmaceutical Fermentations

A simple thin-layer chromatographic method suitable for monitoring microbiological fermentation processes is reported. In addition to compounds related to the target compound the method enables acquisition of data on the formation and degradation of a series of carbohydrates from starch to monosaccharides. The target compounds and the carbohydrates can both be analyzed by use of a single method using one layer only. Both sets of data are useful for optimization of different biosyntheses or bioconversions. The method involves one, effective, thin-layer chromatographic separation, subsequent charring with sulfuric acid solution as detection reagent, heating, and evaluation by scanning the colored spots.