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The Effect of Microwave on Plasmids Amplification and Cell Differentiation in Bacillus Subtilis

ABSTRACT This study was carried out to investigate plasmid amplification and other differentiation by microwave irradiation (1 mV/cm2) and chloramphenicol treatment (170 μg/ml) in Bacillus subtilis. The effect of microwave on B.subtilis was observed and the amounts of DNA, RNA, protein and plasmid were determined during the experiments. The data obtained were evaluated statistically and the variability of the amounts of DNA, Protein, RNA, Growth and plasmid exposed to microwave were less than those treated with chloramphenicol and these amounts significantly differ between microwave and chloramphenicol. Microwave exposure induces any broken fragment and the amounts of plasmids increased.