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Heat properties of an integrated micro PCR vessel

The PCR amplification is based on multiple temperature cycles of DNA synthesis; each includes denaturation of the template, annealing of the primers to complementary sites in the template and primer extension. The key technique of PCR amplification is the heating control in design and fabrication of its chip form. The specifications of the chip are heat properties. In this paper the heat properties of a micro PCR vessel integration heater and temperature sensor was introduced. The temperature distribution of the vessel was simulated with software tool IntelliSuite. The temperatures cycles were measured and the time response of the chip was discussed. It is found that the integrate micro vessel is a very useful tool not only for DNA synthesis but also as a biochemical reactor for many other biological and chemical analyses.