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Analysis of suppressor T cells induced by donor‐specific transfusion (DST): establishment of a human T cell hybridoma producing an antigen‐nonspecific suppressor factor

Formation of suppressor T cells (Ts) induced by donor‐specific transfusion (DST) is one of the most commonly suggested mechanisms for the beneficial effect of DST. In this study, we established a human T cell hybridoma derived from the peripheral blood lymphocytes (PBL) of a DST‐treated patient, which produced an antigen‐nonspecific suppressor factor. Post‐DST PBL were fused with an azaguanine‐resistant mutant of a human T cell leukemia cell line, CCRF‐CEMAG. After selection and cloning, we established one clone producing the mixed lymphocyte reaction (MLR) inhibitory factor (C524: 18%–43 % suppression). Suppressive activity of the supernatant obtained from C524 after activation by PHA was highly augmented (64%–88 % MLR suppression). This factor inhibited MLR dose‐dependently in an antigen‐nonspecific and HLA non‐restricted manner. These results indicated that Ts clones could be generated in patients receiving DST and that the immunoregulatory factors produced by activated clones may play a role in the prolongation of renal allograft survival.