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Simultaneous determination of niacin, niacinamide, pyridoxine, thiamine, and riboflavin in multivitamin blends by ion-pair high-pressure liquid chromatography.

A high-pressure liquid chromatographic procedure for the simultaneous determination of niacin, niacinamide, pyridoxine, thiamine, and riboflavin was developed and applied to the analysis of multivitamin blends for these water-soluble vitamins. Reversed-phase ion-pair chromatography, using sodium hexanesulfonate as the counterion, was employed. Analysis time is shortened considerably and precision is improved by the application of this analytical technique as compared to the current official methods of analysis. Involved sample pretreatment is not required.