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Preliminary Phytochemical Screening and Antimicrobial Activity of the Hydroethanolic Extract of the Fruits of Solanum torvum (Swartz) (Solanaceae) Use as Vegetable in Togo

The fruits of Solanum torvum (Swartz), a vegetable-fruit, are used in traditional medicine in Togo in the treatment of infectious diseases and as an anti-anemic. This study then focused on the antimicrobial activity assessment of the hydroethanolic extract of these fruits in the interest of contributing to the valorization of this Togolese flora’s species. A hydroethanolic extraction (50 % 50 %: v/v) was performed followed by preliminary phytochemical tests. Antimicrobial activity was determined on fourteen bacterial strains using the agar diffusion method. Qualitative phytochemical screening revealed the presence of alkaloids, reducing compounds, tannins, cardiac glycosides, flavonoids, coumarins, triterpenes, saponins, total carbohydrates and free quinones. The extract was active on the reference strains of S. aureus, E. coli, S. pneumoniae and P. aeruginosa. However, this activity was only observed on clinical strains of S. pneumoniae and P. aeruginosa. The extract showed MICs of 25 and 50 mg/ml and BMCs of 50 and 100 mg/ml respectively for S. pneumoniae and P. aeruginosa. The MBC/MIC ratio for these two strains was 2. These fruits would then have bacteriostatic activity on S. pneumoniae and P. aeruginosa. The antibacterial properties of the extract on these germs could justify the use of this plant in traditional medicine for the treatment of certain bacterial infections.


INTRODUCTION
The use of antibiotics in the treatment of bacterial infections is currently limited by the development of antibiotic resistance in bacteria. This bacterial resistance is becoming a growing concern with the proliferation of multi-drug resistant strains such as ESBLs and MRSA, especially in hospitals 1 . Antimicrobial resistance then became a public health issue and a real concern for the scientific communities. The search for new natural antibacterial agents based on medicinal plants, used in the treatment of infectious diseases then became essential 2 .
Among the plants used in antibiotic therapy are Solanum torvum (Swartz) (Solanaceae), a plant native to Central and South America. The species is cultivated as a vegetable in West Africa, Central Africa, South and East Asia. Indeed, Solanum torvum (Swartz) is an edible plant whose fruits are an ingredient in the preparation of certain meals. In addition, the seeds and roots are used in traditional medicine to treat infections and other diseases such as malaria and anemia 3 . In Togo, the fruits of Solanum torvum are used as a fruiting vegetable and are also used in the treatment of anemia. It is therefore necessary to valorize this species in health promotion. It is in this context that this study fits, with the objective to contributing to the valorization of Solanum torvum (Swartz), through qualitative phytochemical screening and evaluation of the antimicrobial activity of the hydroethanolic extract of its fruits.

Study framework
This study was carried out at the Laboratory of Process

Plant material
Fruits of Solanum torvum (Swartz) (Solanaceae) constituted the main plant material of this study. They were harvested in the prefecture of Agoe, at Légbassito (Lomé-Togo), then dried for one week at room temperature and ground to powder using a laboratory mill.

Microbiological material
The

Preparation of the extract
Three hundred grams (300 g) of Solanum torvum (Swartz) fruit powder was macerated for 72 hours in a hydroethanolic solvent of proportion (50 % -50 %: v/v). After maceration, the solution was filtered through a filter paper. The filtrate obtained was evaporated dry under vacuum using a Büchi type rotary evaporator whose water bath temperature was set at 45 °C. The dry extract was weighed and stored in a small glass bottle, labelled and protected from light in the refrigerator for use in the various tests.

Calculation of extraction yield
Yield is the amount of extract obtained from a plant material. It is expressed as a percentage in relation to the dry matter (vegetable powder) and has been calculated according to the following formula: R (%) = M1 x 100/M0 R: Extract yield expressed as a percentage (%) M1: mass of the extract obtained (g) M0: mass of vegetable powder (g)

Preliminary phytochemical screening
Phytochemical screening is a set of qualitative and quantitative tests that allow the presence and quantification of biomolecules such as: alkaloids; flavonoids; tannins; total polyphenols; saponins; carbohydrates; coumarins and triterpenes contained in a plant organ to be detected and quantified. The detection of the presence of these secondary metabolites was carried out either by precipitation reactions and/or coloring of the reaction medium and their quantification by UV-Visible spectrophotometric assays.

Alkaloids research
The detection of alkaloids in the extract was carried out qualitatively following the different methods described by previous studies 4 .

 Dragendorff Test
An aqueous extract solution was treated with a few drops of Dragendorff's reagent (potassium iodide and bismuth solution). The formation of red precipitate indicates the presence of alkaloids.

 Mayer Test
A few drops of Mayer reagent (potassium iodide and mercury) were added to an aqueous solution of the extract. The formation of a precipitate of yellow coloration reveals the presence of alkaloids.

 Wagner Test
When a few drops of Wagner reagent are introduced into an aqueous solution containing extract, the formation of a reddish-brown precipitate indicates the presence of alkaloids.

Saponin research  Foam Test
According to the experimental protocol, 0.5 g of extract was diluted in 2 ml of distilled water contained in a graduated test tube with a total volume of 15 ml. After shaking the solution for 15 seconds, the solution was left to stand for 15 minutes. The formation of a persistent foam layer at least 1 cm thick shows that saponins are present in the samples studied 5 .

Research on reducing compounds
 Fehling test The extract was dissolved in 5 ml of distilled water, then hydrolyzed with diluted hydrochloric acid (HCl) and neutralized with an alkaline solution. After the addition of Fehling's liqueur (reagent A: copper sulfate and reagent B: potassium hydroxide), the mixture was stirred well and then heated in a test tube with a Bunsen burner flame until a red precipitate appeared indicating the presence of reducing compounds 6 .

Tannin research
 Reaction with ferric chloride 1 % To 1 ml of aqueous extract solution, 2 ml of water and 1-2 drops of 1 % ferric chloride reagent were added. The formation of a blue, blue-black or black coloration indicates the presence of gallic tannins; while the formation of a green or dark green coloration corresponds to the presence of catechic tannins 7 .
 Reaction with 10 % lead acetate To 3 ml of aqueous extract was added 1 ml of 10 % lead acetate. The appearance of a whitish or brownish precipitate indicates the presence of tannins in the extract 4 .
 Reaction with ammoniacal copper sulfate A volume of 2 ml of copper sulfate and 2 drops of ammonia are added to 2 ml of the extract. The presence of tannins in the extract is indicated by a blue-green precipitate.

Flavonoids research
Flavonoids were detected after mixing an extract with a few drops of a sodium hydroxide solution (NaOH: 1 %). The formation of an intense yellow color becoming colorless with the addition of diluted hydrochloric acid, corresponds to a positive test 6 .

Research of phytosterols or triterpenes
The extract was treated with a few drops of sulphuric acid

Searching for coumarins
A volume of 2 ml of the extract solution was introduced into a test tube to which 0.5 ml of a 10 % NaOH solution was added. The mixture was heated in a water bath to boiling. After cooling, the tubes containing the heated solutions were observed at 365 nm with a UV lamp. A fluorescent yellow coloration means that coumarins are present in the samples 4-6 .

Identification of total carbohydrates
To 1 ml aqueous extract was added 500 µL of α-naphthol dissolved in ethanol. After mixing, 1 ml of concentrated sulfuric acid is slowly added to the wall of the inclined specimen without mixing to form a layer. A positive reaction is indicated by the appearance of a purple ring at the interface between the acid and the sugar solution 8 .

Cardiac glycoside research
A volume of 2 ml of chloroform is added to 1 ml of the aqueous solution of S. torvum powder, the appearance of a reddish-brown coloration after the addition of sulfuric acid (H2SO4) indicates the presence of cardiac glycosides 7 .

Evaluation of antimicrobial activity of the hydroethanolic extract of Solanum torvum fruits
 Strain treatment The collected strains are grown on appropriate media (Chapman for S. aureus, Mac conkey for E. coli, Sabouraud for C. albicans, Hecktoen for Shigella flexneiri and Salmonella typhimirium, Fresh Blood Gelose for Streptococcus pneumoniae and Chocolate Gelose for Klebsiella pneumoniae) and preserved. These strains will be transplanted on agar medium without inhibitor (nutritive agar) following the recommendations of the French Society of Microbiology (SFM).

 Inoculum preparation
The microorganisms were cultured in Muller Hinton broth for 18 to 20 hours so that they were in the exponential growth phase. Each culture was then suspended in a saline solution of sodium chloride (0.9 % NaCl) at a turbidity equivalent to that of the 0.5 standard on the Mac Farland scale. This suspension, whose OD at 625 nm should be between 0.08 and 0.10, corresponds to approximately 1 to 2.106 CFU/ml and will be used as inoculum for the tests.

 Preparing the test solution
The extract is dissolved in distilled water at a concentration of 100 mg/ml. The sterility of this solution was verified by inoculating an aliquot on Mueller Hinton agar and incubating at 37 °C for 24 hours.
 Culture medium preparation The dehydrated Muller Hinton agar medium (MH) was suspended in distilled water at 36 g/l and then heated in a water bath until completely dissolved. The pH was adjusted to 7.3 ± 0.1 and then the medium was autoclaved at 121 °C for 15 minutes. It was then cooled to 45 -50 °C and poured into sterile 90 mm diameter Petri dishes so that the thickness is 4 mm.
 Antimicrobial testing  Agar diffusion methods Mueller Hinton agars were inoculated by flooding. After drying the plates, the agar is perforated at 6 points of equal distance with a sterile tip cut so that the diameter is 6 mm. The cavities thus formed are filled with 50 µl of the test solution. The plates are incubated in an incubator at 37 °C for 24 hours. The bactericidal action is manifested by the formation of a halo around the wells. The results were read by measuring the diameters of the inhibition zones. The test was carried out twice and an average was made on the two determinations 9 .

 Determination of MIC and WBC by the dilution method
The MIC and WBC were determined for the extracts that were active on germs during the liquid diffusion test.
-Determination of the minimum inhibitory concentration The minimum inhibitory concentration is determined by the macrodillution tube method. MH broth was used to prepare serial dilutions at half concentrations ranging from 50 to 0.390 mg/ml. 10 µL of the inoculum was added to each tube containing 500 µL of test solutions. Tubes without inoculum were considered as negative controls. All these tubes were incubated at 37 °C for 24 hours. The first tube in the series that did not show any visible sign of culture was considered to be MIC 9 .
-Determination of the minimum bactericidal concentration For the BMC determination, an oese was taken from the tubes that showed no visible culture during the MIC determination and seeded on nutrient agar for bacteria and Sabouraud for Candida.
After 24 hours incubation, the lowest concentration of the extract that did not give any colonies is considered as the MBC. In order to determine whether the observed antimicrobial effect is bactericidal or bacteriostatic, the BMC/MIC ratio was performed. A BMC/MIC ratio greater than 1 is considered bacteriostatic and a BMC/MIC ratio less than or equal to 1 is considered bactericidal. Finally, when this ratio is greater than or equal to 16, the activity is non-existent 9 .

Statistical analysis
All results were entered into the EXCEL 2016 spreadsheet program and processed using Graph Pad Prism version 8.4.3 statistical software. The significance level was set at 5 % (p < 0.05). This methodology with the material involved resulted in results that were discussed and conclusions reached. Table 1 shows the extraction yield and physical characteristics of the hydroethanolic extract of the fruits of Solanum torvum.

Results of the preliminary phytochemical screening
The qualitative phytochemical tests carried out on the hydroethanol extract revealed the existence of a variety of secondary metabolites (Table 2).

Results of antimicrobial tests
Antimicrobial test results are recorded in tables 3 and 4.

Qualitative phytochemical screening
Phytochemical screening revealed the presence of numerous compounds. This justifies the use of the fruits of Solanum torvum in many conditions in traditional medicine 12 . Our results are close to those obtained by Kanga et al. 11 who also reported the presence of polyphenols, tannins, flavonoids, saponins and alkaloids. Chah et al. 13 also reported similar results with the presence of alkaloids, flavonoids, saponins, tannins and glycosides.

Antimicrobial activity
The  13 . This difference could be explained by the fact that the solvent used in our study for extraction is a mixture of water and ethanol, whereas Chah et al. 13 used methanol only, the extraction of phytoelements would depend on the type of solvent.
The CMB/CMI ratio carried out with the results on germs showed that the fruit extract of Solanum torvum analyzed would have bacteriostatic activity on S. pneumoniae and P. aeruginosa (CMB/CMI = 2).
Although the CMB/CMI ratio is greater than 1 and considered bacteriostatic, it is not greater than or equal to 16.0. Therefore, the hydroethanolic extract of solanum torvum fruits analyzed can be considered effective against S. pneumoniae and P. aeruginosa. This antimicrobial capacity could be explained by the presence of antimicrobial compounds in the fruits of this plant. Indeed, many pharmacologically bioactive compounds such as alkaloids, flavonoids, tannins, anthraquinones and phenolic compounds are involved in the antibacterial activities of many plants 14 .

CONLUSION
The study of the hydroethanol extract of the fruits of Solanum torvum (Swartz) (Solanaceae) found the presence of numerous bioactive compounds. The extract was active on certain strains of bacteria, justifying its use in traditional medicine for the treatment of numerous infections and other pathologies. It can therefore be used in the development of improved traditional medicines, in a context of resistance of bacterial strains against conventional antibiotics.

Sources of support: None
Ethical consideration: Not applicable