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[SH group content and hydrolase activity of the small intestine mucosa in chickens fed a mixture containing aflatoxin B1].

The effect of aflatoxin B1 on the content of SH-groups and the activity of leucinamino peptidase, Mg (Na+, K+), ATP, and glucoamylase in the mucous membrane of the small intestine of birds was studied. The experiment was carried out with three groups of male broilers. The controls were given starter and finisher mixtures with 21.6 and 19.5 per cent protein, with no aflatoxin B1. The birds of the test groups (II and III) were offered one and the same mixture containing 0.250 and 0.600 ppm of aflatoxin B1. By the end of the finisher period on the 49th day mucosa homogenate was used to determine the content of SH-groups and the activity of leucinamino peptidase, Mg (Na+, K+), ATP, and glucoamylase. It was found that rates of 0.600 ppm in the feed suppressed the activity of Mg (Na+, K+) ATP in the mucosa of the small intestine. Lower amounts (0.250 ppm) produced no effect on the activity of this enzyme. Leucinamino peptidase, glucoamylase, and the SH-groups did not change essentially their activity at the two rates of the toxin.