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In Vitro Susceptibility Comparisons and Recommendations for Oxolinic Acid

Minimal inhibitory concentration and disk diffusion susceptibility studies were carried out with oxolinic acid to determine a recommended disk content for disk susceptibility testing. Regression curve analyses were performed with disks containing 1, 2, and 5 μg of oxolinic acid. Data suggest that the 5-μg disk more satisfactorily fulfills requirements for susceptibility testing than the 1- or 2-μg disks evaluated. Ultraviolet spectral analysis studies for absorption maxima and extinction coefficients were done to verify the authenticity and concentration of oxolinic acid in the aqueous solutions studied.