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Sensitivity of Extracellular Vesicles from Human Blood Serum to Various Detergents

Blood exosomes and microvesicles, collectively known as small extracellular vesicles (sEV), are vesicles about 100-150 nm in size. Small 
EV are involved in various aspects of signaling in the body; in addition, they can serve as markers of various pathologies. For biochemical studies, 
vesicle solubilization is often required. We tested the ability of various detergents to dissolve membranes of the sEV. Small EV were isolated from 
the blood serum of healthy volunteers by gel filtration on Sepharose CL-2B and tried to solubilize them using the anionic detergent DOC (sodium 
deoxycholate), non-ionic detergent Brij 35 (polyoxyethyleneglycol dodecyl ether), zwitterionic detergent CHAPS (3 - [(3-chloramidopropyl) 
dimethylammonio] -1-propanesulfonate), and cationic detergent CTAB (cetyl trimethylammonium bromide). The concentration of sEV in the 
solution was determined by dynamic light scattering. We find DOC is the most effective for sEV solubilization.