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Leukocyte Contamination of Red Cells in Leukocyte‐Poor and Frozen‐Deglycerolized Units

Red blood cell recovery was better and removal of white cells equally good when units were centrifuged briefly in an upright position as compared to more prolonged centrifugation in an inverted position. Nylon fiber filtration left more white cells than did differential centrifugation; a combination of the two technics provided the smallest amount of white cell contamination. Red blood cells frozen and deglycerolized by the original Huggins process had as many leukocytes as those prepared by differential centrifugation. The current Huggins process left more white cells than did the original method.