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Visualization of active ingredients uptake in seed coats with stimulated Raman scattering microscopy

The tissues surrounding the seeds play an important role in the control of germination vigour and in the uptake of active ingredients (AIs) applied as seed dressings. The seeds can reduce costs through more efficient germination rates and have less environmental impact due to more efficient use of AIs. In this study, we use epi-detected stimulated Raman scattering (SRS) microscopy with a fiber laser source for analyzing modes of D2O into intact seeds and determining uptake kinetics. We demonstrate that SRS microscopy is an ideal tool for visualizing uptake of AIs and worthy of further applications in real field conditions.