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A Rapid Method for Separating Tomato Polygalacturonases by HPLC


A new procedure has been developed for separating the 2 polygalacturonases (PG I and PG II) in ripe tomatoes (Lycopersicon esculentum Mills cv. Better Boy). It involves high performance liquid chromatography of tomato extracts on a short column of a strong cation exchanger and elution with a salt gradient. The advantages of this procedure are that resolution of the polygalacturonases is achieved in only 20 min, and the extracts containing low levels of activity can be analyzed.