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Giant cell arteritis.

Burg et al' and Savva and Holliman2 recently described the use of the polymerase chain reaction (PCR) to detect Toxoplasma gondii. We have also been using amplification reactions to detect the presence of this parasite in clinical specimens since 1989. Our method is different from that described by these authors, being based on a different target gene. We use a ribosomal DNA (rDNA) sequence, initially described by Johnson et al in phylogenetic studies.3 Indeed, in eucaryotes the rDNA is frequently repeated and its sequence consists of a patchwork of highly conserved and specific segments. The primers were chosen to match the