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The Difference of mRNA Expression of ATP-Sensitive K^+ Channel Subunits in Embryonic and Adult Mouse Heart

ATP-sensitive K + (K A T P ) channels is expressed in early-stage embryonic heart as well as at the adult stage. K A T P channels are composed of pore-forming (Kir) and regulatory sulfonylurea receptor (SUR) subunits. We studied mRNA expression of Kir6.1, Kir6.2, SURI, SUR2A, SUR2B and SUR2C mRNAs encoding for K A T P channels in cardiac ventricles of embryonic (9.5 days postcoitum, dpc) and adult mice using RT-PCR. 30 cycles of PCR revealed mRNA expression of all genes at the adult-stage. At 9.5 dpc, Kir6.1 mRNA was undetectable, whereas mRNAs of other subunits were expressed. At 9.5 dpc, SUR2A expression was higher than SUR1 and SUR2B. In the adulthood, mRNAs of all subunits were detected. This finding suggests that Kir6.2 is dominant pore-forming subunit from an early embryonic stage. The developmental changes of transcriptional profile of K A T P channels may underlie the different functional properties of the channel in pre- and post-natal period.