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The prognosic value of the downregulation of leukocyte cell-derived chemotaxin 2 gene of hepatocellular carcinoma.

10559 Background: Vascular invasion of hepatocellular carcinoma (HCC) is the major contribution of high recurrence and poor survival. In our previous study, we used suppression subtractive hybridization and cDNA microarray to identify leukocyte cell-derived chemotaxin 2 (LECT2) as one of the 20 differentially-expressed genes between vascular-invasion, and non-vascular invasion of HCC. We also found LECT2 gene expression level was correlated with inhibitory effect of invasion ability of hepatoma cells through gene over-expression and knockdown experiments. The purpose of this study is to identify the relationship of gene expression level of LECT2, and clinicopathologic outcome in human HCC.


METHODS
127 patients with primary, unifocal HCC were included. Archives HCC tumor tissue samples were used after approval by institutional review board and written informed consent. RT-PCR and western blot were used to measure LECT2 gene and protein expression level of HCC. The correlation with clinicopathologic outcome were analyzed by statistical methods.


RESULTS
Down-regulation of LECT2 gene in HCC is correlated with high tumor stage, more vascular invasion (p<0.0001), and early recurrence (p< 0.005). There's no significant difference with patients' age, gender, HBsAg, AFP level. We also found LECT2 down-regulation is positively correlated with p53 mutation, and osteopontin expression, negatively correlated with beta-catenin mutation of the tumor tissues. Patients with HCC of low LECT2 gene expression level have poor overall survival by Kaplan-Meier estimate (p=0.005).


CONCLUSIONS
Down-regulation of leukocyte cell-derived chemotaxin 2 (LECT2) gene of hepatocellular carcinoma is a poor prognostic factor.