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Endoplasmic reticulum stress affected chondrocyte apoptosis in femoral head necrosis induced by glucocorticoid in broilers

ABSTRACT In our previous study, chondrocyte apoptosis in femoral head necrosis (FHN)‐affected broilers was found to be associated with the endoplasmic reticulum stress (ERS) signaling pathway. In the present study, we further explored the role of ERS‐induced chondrocyte apoptosis in FHN‐affected broilers and the parallel test was carried out with articular chondrocytes cultivated in vitro. The broilers and chondrocytes were treated with methylprednisolone (MP). The main pathological changes in FHN‐affected broilers included the proximal femoral head separated from its articular cartilage and growth plate lesions. MP‐treated chondrocytes demonstrated morphology changes, cell viability reduction, secretory capacity dysfunction, and apoptosis. The mRNA expressions of pro‐apoptotic genes controlled by ERS signaling pathway were up‐regulated both in vivo and in vitro experiments. It showed that MP induced FHN in broilers, activated apoptosis‐related genes on ERS signaling pathway, and affected the survival and apoptosis of chondrocytes, and bone growth.