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Simultaneous determination of chloramphenicol and salicylic acid in tincture by HPLC

Objective: To establish the HPLC method for simultaneous determination of chloramphenicol and salicylic acid in tincture. Methods: HPLC was used in the quantitative analysis with C18 chromatographic column(4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-water-glacial acetic acid(50 : 50 : 1) with the flow rate of 1.0 m L·min-1. The column temperature was 30 ℃. The detection wavelength was 295 nm and the injection volume was 10 μL. Results: The calibration curves of chloramphenicol and salicylic acid had a good linearity in the range of 60.77 – 141.80 μg·m L-1(r = 0.999 8) and 123.50 – 288.10 μg·m L-1(r = 0.999 7) respectively. The average recovery of chloramphenicol was 99.76% with RSD of 1.08%(n = 9). The average recovery of salicylic acid was 99.87% with RSD of 1.19%(n = 9). Conclusion: The method is simple, accurate and reliable, which can be used as the quality control method of this preparation.