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Detection of Salmonella Enteritidis (SE) Bacterin in Environmental Samples from Poultry Houses Using the RapidChek SE Immunoassay

Important Findings Environmental swabs from pullet houses were tested for the presence of Salmonella Enteritidis (SE) using the RapidChek SE immunoassay test. Of the 1162 samples tested in 2011, 20 samples were positive, but only two samples were confirmed positive for SE by culture. Furthermore, swabs experimentally inoculated with as little as half a dose (0.25 ml) of a commercial killed SE vaccine were positive for SE using the mentioned immunoassay. This finding emphasizes the need to perform bacterial isolation and identification when a positive result occurs using an immunoassay test.