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Expression of Vascular Endothelial Growth Factor (VEGF) Isoforms VEGFxxxb in Normal Human Renal Development

The VEGF‐A gene is alternatively spliced resulting in the generation of several angiogenic VEGF‐A isoforms, termed VEGFxxx, and anti‐angiogenic isoforms termed VEGFxxxb. VEGFxxx isoforms are generated by a proximal splice site selection in exon 8, whereas the VEGFxxxb isoforms are generated by a distal splice site selection in exon 8. The aim of this study was to characterise VEGFxxxb localisation in normal fetal kidney at 6, 10, and 12 weeks gestation, and adult human tissues using immunohistochemical staining. 5μm thick sections of routinely fixed tissues samples were subjected to immunohistochemical staining using a VEGFxxxb specific antibody before detection with biotinylated secondary antibody, avidin‐biotin enzyme complex and diaminobenzidine substrate. Immunohistochemical staining revealed in the mesonephrois, VEGFxxxb isoforms were localised to cuboidal epithelial cells of the excretory tubules and to podocytes of the glomerulus. In the metanephroi, distinct patterns of VEGFxxxb staining were observed at specific stages of nephron differentiation and maturation. In the adult kidney, strong VEGFxxxb staining was observed in the glomerular podocytes and some epithelial cells of the Bowman’s capsule. The data presented herein demonstrates that the VEGFxxxb isoforms are present in both fetal and adult kidney tissue, with the degree dependent on maturation and cell type. Welcome Trust Grant 69029