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[Biocompatibility study on tendon mixed extraction of bovine collagen for periodontal tissue engineering].

OBJECTIVE
To study the biocompatibility of tendon mixed extraction of bovine collagen (tMEBC) and to explore the feasibility of using the three-dimensional framework as periodontal tissue engineering scaffold.


METHODS
After being prepared, the tMEBC were cultured with the P4-P6 of human periodontal ligament fibroblasts (HPDLFs) in vitro. Three-dimensional framework was prepared from bovine tendon. The P4-P6 of HPDLFs (with an initial density of 5 x 10(6) cells/ml) were cultured in vitro. Cell attachment and proliferation were measured by cell counting 1 day, 3, 5, and 10 days after cell seeding. Histological examination was performed with light microscope and scanning electron microscope 5 and 10 days after cell seeding.


RESULTS
Porous structure, which supported the proliferation and attachment of HPDLFs, was found in tMEBC. The density of cell increased from 0.556 x 10(4) cells/ml 24 hours after cell seeding to 3.944 X 10(4) cells/mI 10 days after seeding. Light and scanning electron microscope examination indicated that HPDLFs were attached and extended on the three-dimensional scaffolds and were well embedded in the newly formed tissue matrix.


CONCLUSION
tMEBC has good biocompatibility with the HPDLFs, and can be used as scaffold for cell transplantation in periodontal tissue engineering.