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BAFF‐R gene induced by IFN‐γ in multiple myeloma cells is related to NF‐κB signals

B‐cell activating factor (BAFF) is a potent cell‐survival factor expressed in many haematopoietic cells. BAFF regulates B‐cell survival, differentiation and proliferation by binding to three tumour necrosis factor receptors: transmembrane activator, calcium modulator and cyclophilin ligand interactor; B‐cell maturation antigen; and BAFF receptor (BAFF‐R). Although BAFF‐R is produced by interferon gamma (IFN‐γ), the underlying mechanism remains elusive. In this study, we examined the effects of IFN‐γ on BAFF‐R expression in cultured human multiple myeloma cells (KM3) both at the transcriptional and posttranscriptional levels. Incubation of KM3 cells with IFN‐γ elevated the expression of BAFF‐R mRNA and protein levels. IFN‐γ elicited marked enhancement of the human BAFF‐R promoter activity and nuclear factor kappa B (NF‐κB) DNA binding activity. NF‐κB dependent on the human BAFF‐R gene might be regulated via a transcriptional event through one putative NF‐κB site on the BAFF‐R gene promoter. These results provide a molecular mechanism for the increase in expression of the BAFF‐R gene that is induced by proinflammatory cytokines in responsive cells. Copyright © 2011 John Wiley & Sons, Ltd.