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[Simultaneous determination of three antioxidants BHA, BHT and TBHQ in food by liquid chromatography and gas chromatography].

OBJECTIVE
To develop a method for determining 3 kinds of antioxidants in food in same time with HPLC and GC.


METHODS
Firstly, extracted fat with petroleum ether, then extracted antioxidants with 13 ml methnol, centrifuged, repeated the above procedure twice, combined the extracts, next evaporated to 5 ml, constanted volume to 10 ml, then kepted in freezer (- 1h), lastly, the supernatant injected into high performance liquid chromatography (HPLC) and Gas chromatography (GC) which had been optimized condition.


RESULTS
The limits of quantitation for BHA,BHT and TBHQ were 0.002, 0.010 and 0.002g/kg respectively in HPLC, The limits of quantitation for BHA, BHT and TBHQ were 0.003, 0.002 and 0.005 g/kg respectively in GC, The mean recoveries at the two spiked levels were 82.8% - 109.0%. There were no significant difference between HPLC and GC.


CONCLUSION
The method can be used to determine the antioxidants in food accurately and sensitively, the comparative results are constant between liquid and gas chromatography.