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A and B and A1Leb Substances in Glycosphingolipid Fractions of Human Serum 1

Abstract. A and B and A1Leb substances were adsorbed onto red cells exposed to glycosphingolipid fractions prepared from the serum of group A and B and A1, Le(a‐b+) donors. Group O cells exposed to fractions prepared from the serum of group A or B donors were agglutinated by an IgM cross‐reacting antibody present in some group O sera. Cells exposed to fractions from A1, Le(a‐b+) serum were agglutinated by anti‐A1Leb. The amount of A substance in the fractions was related to the A subtype (A1 or A2) and to the Lewis and secretor phenotype of the donor. The uptake of blood‐group substances from the lipid fractions was inhibited by the addition of whole serum to the fractions.