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Measurement techniques for melanoma: a statistical comparison

Inter- and intra-observer variation in measuring the depth of invasion of malignant melanomas was assessed using three different techniques: eye-piece graticule, stage Vernier, and projection image analysis. Significant variation was found for all methods but was least pronounced with the stage Ver- nier. It is recommended that this should be the preferred technique for routine use. It has been clearly established that the depth of invasion of the dermis is the single most important prognostic factor in determining the outcome of any melanoma.' It is therefore essential to have reliable, accurate techniques for the measurement of this. This study examines intra-observer variation in assessment of the Breslow thickness2 using three different measurement techniques: the eye- piece graticule, the stage Vernier, and projection image analysis, and inter-observer variation using the same three techniques perfor-med by two pathologists (CJC and APC).