{'fraction_non_alphanumeric': 0.04806595912639242, 'fraction_numerical': 0.04789053591790194, 'mean_word_length': 4.790756729304215, 'pattern_counts': {'":': 0, '<': 18, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 14, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Association of Age and Sex with Metabolic Syndrome in Taiwanese Adults

Objective This study aimed to investigate the association of age and sex with metabolic syndrome (MS) in Taiwanese adults. Methods We extracted information of 4307 men and 4783 women aged 30–70 from the Taiwan Biobank. Results The interaction between age and sex on MS was significant (p-value = 0.0001). After stratification by sex, men and women aged 50–70 years (reference: 30≤age<50 years) had a higher risk of MS. The odds ratio (OR), 95% confidence interval (CI) was 2.316, 1.936–2.772 in men and 3.101, 2.561–3.754 in women. After stratification by age, men aged 50–70 years had a lower risk of MS compared to women (OR, 95% CI = 0.713, 0.598–0.851). Conclusion The interaction between age and sex on MS was significant. Sex-wise, both men and women aged 50–70 years had a higher likelihood of MS. Age-wise, men aged 50–70 years had a lower risk of MS compared to women.


Study Population
Data were obtained from the Taiwan Biobank, whose participants are Taiwanese men and women between 30 and 70 years, who have never been clinically diagnosed with cancer. The Taiwan Biobank was founded to collect biological, lifestyle, and genetic information to determine health risk factors and establish research resources. Information about enrolment into the Taiwan Biobank project is available through media, posters, brochures, and websites. Currently, 29 centers have been allocated all over Taiwan for recruiting participants. Noteworthy, each city or county has at least one center. Taiwan Biobank complies with relevant data protection and privacy regulations. After interested volunteers sign the consent form, researchers interview them and fill questionnaires on age, sex, lifestyle (e.g, cigarette smoking, exercise, and alcohol drinking), and others. Moreover, trained and qualified researchers collect blood samples to determine parameters like fasting blood glucose (FBG), highdensity lipoprotein (HDL), and triglyceride (TG). Anthropometric data (eg, waist circumference, weight, and height), blood pressure, pulse, and others are determined by physical examinations.
In this cross-sectional study, we included a total of 9090 individuals with body mass index (BMI) ≥18.5kg/ m 2 having complete data. The participants were stratified into two age cohorts (30 to < 50 and 50-70 years). All participants signed the informed consent letter. The Institutional Review Board of Chung Shan Medical University Hospital approved this work (CS1-20009).

Definition of Metabolic Syndrome
Metabolic syndrome was defined according to the criteria of the Ministry of Health and Welfare in Taiwan. Participants were classified as having metabolic syndrome if they met more than three of the following conditions: (1) waist circumference ≥ 90 cm for men and ≥ 80 cm for women; (2) systolic blood pressure (SBP) ≥ 130 mmHg or diastolic blood pressure (DBP) ≥ 85mmHg; (3) fasting blood glucose ≥ 100mg/dl; (4) high-density lipoprotein < 40 mg/dl for men and < 50 mg/dl for women; and (5) triglyceride ≥ 150 mg/ dl. The tools used to assess the above variables were 1) measuring tape, for waist circumference; 2) blood pressure monitor (OMRON HEM7320, HEM7210), for blood pressure; 3) clinical analyzer (Hitachi LST008), for fasting blood glucose, HDL, and triglycerides. The tests were performed at the Chang Gung Memorial Hospital, Linkou, Taiwan following the manufacturers' instructions.

Description of other Variables
Information on cigarette smoking, alcohol drinking, exercise, midnight snack, coffee, vegetarian diet, and fat intake was self-reported. For cigarette smoking and alcohol drinking, we categorized participants into three (current, former, non-smokers or drinkers). Nonsmokers were those with no personal history of smoking or those who have never smoked continuously for at least 6 months. Former smokers were individuals who quit smoking but have ever smoked continuously for a minimum of 6 months. Current smokers were active smokers who have been continuously smoking for at least 6 months. Non-drinkers included individuals with no history of alcohol drinking or those with a weekly intake of < 150 cc of alcohol continuously for 6 months. Former drinkers included those who abstained from alcohol for at least 6 months. Current drinkers included active alcohol drinkers with a weekly alcohol intake of at least 150 cc for continuously 6 months. Doing exercise which lasted for a minimum of 30 minutes at least three times per week was considered as regular exercise. Coffee drinking was defined as consuming coffee at least three times a week within the past month. Midnight snack takers were defined as those who had a habit of eating snacks one hour before bedtime even after eating dinner. Vegetarians included individuals who continuously followed a vegetarian course for 6 months or more. Fat intake was determined using fat scores (0-30) derived from the frequency of eating fat-rich food within the past month. We categorized participants into quartiles: <Q1 (score<13), Q1-Q2 (13≤score<15), Q2-Q3 (15≤score<18), and ≥Q3 (score≥18).
We calculated BMI as weight in kilograms (kg) divided by height in meters squared (m 2 ). Height was determined with a manual height meter while the weight was determined using Tanita body composition analyzer (BC-420MA). BMI categories included normal weight, overweight, and obesity corresponding to 18.5≤BMI<24, 24≤BMI<27, and BMI ≥ 27 kg/m 2 .

Statistical Analysis
We used SAS version 9.4 (SAS Institute Inc., Cary, NC) to analyze data. Demographic data between metabolic syndrome cases and non-cases were compared using Student's t-test (for continuous variables) and chi-square test (for categorical variables). The association of age and sex with MS and the interaction between sex and age on MS were determined using multiple logistic regression. Confounding variables included cigarette smoking, alcohol intake, BMI, exercise, midnight snack intake, coffee intake, vegetarian diet, and fat intake. P-values < 0.05 were considered as being statistically significant. Table 1 shows the demographic information of the study participants with and without MS. Age, sex, smoking, alcohol drinking, BMI, and coffee intake were significantly different between cases and non-cases of MS (p-value <0.0001). MS was significantly associated with age and sex: age (50-70 compared to 30 to < 50 years) was associated with a higher risk (OR = 2.615, 95% CI = 2.298-2.975) while sex (men compared to women) was associated with a lower risk

DovePress
(OR = 0.810, 95% CI = 0.705-0.929). There was also a significant interaction (p-value = 0.0001) between sex and age on metabolic syndrome (Table 2). With participants stratified by sex, the age group 50-70 years (reference: 30≤age<50 years) was significantly associated with a higher risk of MS in both sexes (  (Table 3). With participants stratified by age, there was no significant relationship between sex (men compared to women) and MS among participants aged 30 to < 50 years. However, the risk of MS was significantly lower among men (compared to women) aged 50 to 70 years: OR = 0.713, 95% CI = 0.598-0.851 (Table 4).
With both sex and age combined and the reference group being women aged 30 to < 50 years, the risk of MS was significantly higher among both men and women aged 50 to 70 years. The ORs (95% CIs) were 3.387 (2.809-4.083) in women and 2.279 (1.863-2.788) in men ( Table 5).

Discussion
Many studies conducted within the last two decades have reported inconsistent findings regarding the link between sex and MS. [9][10][11][17][18][19][20][21][22][23][26][27][28][29] Previous studies among Taiwanese adults revealed significant associations between sex and MS and also an interplay between sex and age on the risk of MS. 10,11,22,32,38,40 In the current study, we confirmed the individual association of sex and age with MS as well as an interplay of both factors on MS.
Based on data from the Nutrition and Health Survey in Taiwan (NAHSIT), the prevalence of MS increased from 13.6% between 1993 and 1996 to 25.5% between 2005 and 2008. 11 The prevalence of MS among adults undergoing health checks between 2000 and 2001 at MJ health screening centers was 12.9%. 22 Moreover, according to data from nationwide MJ Health Screening Centers, the prevalence among healthy nonalcoholic Taiwanese adults was 10.1% in 2000. 45 In a nationwide cross-sectional study in 2002, the prevalence was 15.7%. 10 In another cross-sectional study in 2004, the prevalence among residents of a metropolitan city aged 40 years and over was 30%. 33 In other studies, the prevalence was 8.2% among company workers in Southern Taiwan in 2008, 23 12.1% among workers in Changhua County during 2008-2012, 37 12.0% among hospital employees in northern Taiwan in 2011, 49 and 16.6% among company workers in Central Taiwan in 2012. 50 The increase in the prevalence of MS over the past two decades could be attributed to an increase in the aging population, industrialization, and urbanization. The sex-difference (low risk in men relative to women) in MS confirmed in this study is in line with previous studies. 10,20,22,23,26,32,37,45,46,48,[51][52][53] Even though a recent study among Taiwanese adults also showed a lower risk in men than women, the results were not statistically significant. 54 The significant sex-age interaction on MS has also been confirmed in some studies. 10,11,18,22,28,32,33,38,40 Like our results, age was directly associated with the prevalence of MS in Taiwanese 10,51,52,54,55 and other populations. 52,53,56,57 However, unlike our study, the prevalence in women was initially lower than in men but later increased rapidly, surpassing that in men, with a crossover effect occurring

DovePress
between both sexes at 60-69 years. 10 Moreover, the prevalence in women aged < 45 years was lower than in men of the same age group, and there was a crossover between both sexes after age 45. 11 Furthermore, women had a lower risk of MS compared with their male counterparts but became more vulnerable than men at and beyond 50 years. 38 This study is limited in that we used qualitative rather than quantitative data to assess variables like smoking and exercise due to the unavailability of these data. Moreover, there was no information on the type of coffee or how coffee was consumed.

Conclusion
The interconnection between sex and age on MS among Taiwanese was confirmed in this study. Sex-wise, both men and women between 50-70 years had a higher likelihood of MS. However, the risk was much higher in women compared to men. Age-wise, men aged 50-70 years had a lower risk of MS compared to women of the

Disclosure
The authors report no conflicts of interest in this work.

Publish your work in this journal
The International Journal of General Medicine is an international, peer-reviewed open-access journal that focuses on general and internal medicine, pathogenesis, epidemiology, diagnosis, monitoring and treatment protocols. The journal is characterized by the rapid reporting of reviews, original research and clinical studies across all disease areas. The manuscript management system is completely online and includes a very quick and fair peer-review system, which is all easy to use. Visit http://www.dovepress.com/ testimonials.php to read real quotes from published authors.