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Evaluation of a filter-syringe set for preparation of packed cell aliquots for neonatal transfusion.

A closed-system filter-syringe set designed for preparation of prefiltered aliquots of packed red cells for neonatal transfusion was evaluated. In three experiments, filter-syringe sets were sterile-connected, and aliquots were prepared at six intervals during 35-day storage of CPDA-1 packed cells. Hemoglobin (HGB), supernatant potassium (K+), and free hemoglobin (fHGB) in the aliquot and primary storage container were compared. There was no tubing weld failure, filter-occlusion, or bacterial contamination of the units. Hemoglobin remained stable over 35 days of storage, with comparable values in the primary collection container and syringe aliquot. Supernatant potassium in the units increased to an average of 89.5 mEq/L at 35 days, and levels in the aliquots closely matched those in the primary containers at each storage interval (maximum average 91.8 mEq/L). Free hemoglobin also progressively and comparably increased in both the primary containers and syringe aliquots. The gentle negative pressure and turbulence during use of the filter-syringe set apparently causes little hemolysis. The set simplifies preparation of aliquots for neonatal small volume and syringe-pump transfusion at costs comparable to alternative preparation methods. When sterile-connected, the filter-syringe set facilitates "assigned unit" inventory management for neonatal transfusion, which has been shown to be highly effective in limiting donor exposures.