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Distribution of ITI phenotypes in the Iberian Peninsula: Negative correlation between ITI*3 frequencies and latitude in human populations

The molecular phenotypes of ITI were analysed in samples from seven populations of the Iberian Peninsula (Galicia, Central Portugal, Castilla‐Leon, Castilla‐La Mancha, Extremadura, Western Andalusia, and Eastern Andalusia) by means of IEF in ultrathin layer polyacrylamide gels followed by immunofixation‐silver staining. The observed ITI*3 frequencies exhibit the highest values thus far reported in European populations. Regression analysis between ITI*3 frequencies and latitude reveals a negative correlation (r = −0.8798, P < 0.001). © 1994 Wiley‐Liss, Inc.