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Modification of Fixation Process in Avian Histologic Sections

Abstract Preparing avian tissue samples in histology laboratories involves a problem we usually confronted with: the process, although the same in the case of all other animals, usually results in widespread artifacts, which requires us to repeat the test, usually without the desired result. Considering the artifact types, the major problem seems to result from an inefficient fixation process of the samples. Therefore, to modify the process, samples of kidney, lung, skin, proventriculus, esophagus, duodenum, jejunum, ileum, cecum, spleen, and bursa of fabricious of healthy mature native hens were taken immediately after slaughter and fixed with buffer formalin at 5%, 7.5%, lo%, and 15%. Then, other histological laboratory procedures were similarly performed for all samples and the 6-μm sections were stained with hematoxylin–eosin and studied under light microscope. The repeated tests on new samples of the same organs in the same conditions and with the same procedures for a sure and reliable result proved the buffer formalin at 15% the best for all the examined tissues except for the lung, for which 7.5% to 10% proved the best. (The J Histotechnol 29:123, 2006) Submitted January 7, 2006; accepted with revisions April 10, 2006