{'fraction_non_alphanumeric': 0.04333694474539545, 'fraction_numerical': 0.028710725893824486, 'mean_word_length': 4.338150289017341, 'pattern_counts': {'":': 0, '<': 3, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Relationship of protease-activated receptor-2 and mast cell in the rat model of small intestinal mucosal damage by short-term administration of non-steroidal anti-inflammatory drug].

OBJECTIVE
To identify the expression and significance of protease-activated receptor-2 (PAR-2) and mast cell (MC) in the rat model of small intestinal mucosal damage by a short-term administration of diclofenac.


METHODS
Twenty-four SD-rats were divided into 2 groups (control group and model group, 12 rats each) by random digit table. The rats in the control group were treated with 1 ml distilled water per 250 g, once a day while those in the model group diclofenac 7.5 mg/kg per day. Their terminal ileum was harvested at Day 5 after an intraperitoneal injection. Toluidine blue dyeing was employed to determine the distribution of MC and its count in intestinal mucous membrane. Immunohistochemistry, Western blot and real-time PCR (polymerase chain reaction) were employed analyze the location, expression and change of PAR-2 mRNA in intestinal mucous membrane.


RESULTS
The count of MC was obviously higher in the model group than that in the control group (10.3 ± 2.2 vs 4.2 ± 1.2, P < 0.05). PAR-2 was expressed on the mucous surface, recesses epidermis and lamina propria inflammatory cells. And positive dyes were located within cytoplasm. As compared with the control group, the expression of PAR-2 mRNA was higher (2.63 ± 0.26 vs 1, P < 0.05) and its protein expression higher in the model group (24.3 ± 2.4 vs 17.5 ± 3.5, P < 0.05).


CONCLUSION
Both PAR-2 and mast cell are involved in the pathogenesis of small intestinal mucosa injury induced by diclofenac in rats. PAR-2 may be activated by tryptase released from mast cells and participate in the pathogenesis of small intestinal injury as induced by non-steroidal anti-inflammatory drugs.