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[Inflammatory reaction to polyester material in the guinea pig. IV. Production and liberation of beta glucuronidase and lactate dehydrogenase by peritoneal macrophages in culture].

Peritoneal macrophages obtained from guinea pigs intraperitoneally injected with minced polyester threads with saline, and from control animals were cultivated and the activities of the lysosomal enzyme beta-glucuronidase and of the cytoplasmic enzyme lactate dehydrogenase were determined in the cells and in the culture media. The production and particularly the release into the medium of beta-glucuronidase increased in the cultures from the treated animals; the LDH production was also markedly increased, and the enzyme was partly lost into the medium, suggesting that the injection of Mersilene into the guinea pigs markedly induces these enzymes in the phagocytes and at the same time probably increases the leakiness of their cell membrane.