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Imprinted-Zeolite-X-Based Sensor for Non-Enzymatic Detection of Blood Glucose by Potentiometry

The development of sensors based on imprinted zeolite X to detect blood glucose through potentiometry was performed. In this study, the sensor was made of a mixture of carbon paste and imprinted zeolite X. Zeolite X was synthesized using a sol–gel-hydrothermal method at a temperature of 100 °C with basic materials of NaAlO2, NaOH, TEOS, and distilled water. The characterization results of XRD showed the presence of specific peaks, which were confirmed with standard zeolite X. Imprinted zeolite X exhibited a 20 times greater adsorption capacity size, and an adsorption efficiency 3 times greater than that of zeolite X. This is thought to be due to the presence of a molecular template within it. The IZ–carbon paste electrode showed optimum performance due to a mass ratio of carbon, paraffin, and imprinted zeolite X of 12:7:1. The electrode performance was expressed by the Nernst factor value of 30 mV/decade, the measuring range of 10−4–10−2 M, the upper detection limit of 1.38 × 10−2 M, and the lower detection limit of 1.28 × 10−4 M, so this electrode can be used for glucose analysis with a normal concentration (70–110 mg/dL or equivalent to 3.8 × 10−3–6.1 × 10−3 M), as well as the glucose concentration of people with diabetes mellitus (>200 mg/dL or about 10−2 M). This electrode showed precision values of 97.14–99.02%, accuracy values of 98.65–99.39%, and electrode response times of 10–13 s. The electrodes showed high stability for more than 5 weeks with 141 uses. The electrodes also showed high selectivity for glucose in the matrix of uric acid, urea, NaCl, and KCl. Therefore, its use as an alternative electrode for routine glucose analysis in the medical field is recommended.