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Preharvest Sprouting in the 2002 Midwestern Barley Crop: Occurrence and Assessment of Methodology

The objective of this research was to compare pearling with other techniques for the assessment of preharvest sprouting (PHS). The 2002 Midwestern crop provided a unique opportunity to evaluate PHS methodology because a large amount of sprouted samples from a limited varietal and geographic base were available. Approximately 30% of the samples collected in Minnesota and northern districts of North Dakota were sprouted. All samples (N = 285) were evaluated by pearling and stirring number (SN), while smaller subsets were utilized for the other methodology. Falling number and SN were most sensitive for the detection of low levels of PHS. Pearling and staining with fluorescein dibutyrate were less sensitive to low levels of PHS. The measure of conductivity was found not strongly related to other measures of PHS. Samples were stored at ∼20°C for up to 11 months following harvest. A decline in the germinative energy (GE) of some samples was observed and, in general, this was most severe for sprouted samples. While significant correlations were observed between GE and measures of PHS, in no case was a correlation of a magnitude where a measure of preharvest sprouting alone could be used to predict seed storability with an acceptable degree of accuracy.