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Adhesion of trophoblast to uterine epithelium as related to the state of trophoblast differentiation: In vitro studies using cell lines

At the initial phase of embryo implantation, the trophoblast must have acquired competence for adhesion to the uterine epithelium, a condition whose cell biological basis is far from understood. In the present study, trophoblast‐type cells (BeWo, JAr, and Jeg‐3 choriocarcinoma cell lines) were treated with retinoic acid, methotrexate, dibutyryl‐cAMP, or phorbol‐12‐myristate‐13‐acetate in order to modulate their ability to adhere to uterine epithelial cells (RL95‐2). In an established model, multicellular spheroids of choriocarcinoma cells were transferred onto the surface of monolayer cultures of RL95‐2 cells followed by a centrifugal force‐based adhesion assay. In controls, about 45% of BeWo and JAr cell spheroids and 75% of Jeg‐3 spheroids adhered to uterine monolayers within 30 min. Pretreatment of spheroids with either of the agents stimulated differentiation as indicated by the rate of chorionic gonadotropin secretion, but consistently reduced the adhesion to the endometrial monolayer in all three choriocarcinoma cell lines. While previous investigations had shown that invasiveness of trophoblast cells (into extracellular matrix) does not seem to be linked to the differentiation program in a simple manner, the present data suggest that such an (inverse) link may indeed exist with respect to the ability to initiate an adhesive interaction with the uterine epithelium. These observations support the view that epithelial cell interactions as typical for the initial phase of embryo implantation are regulated in a way that is clearly different from cell–matrix interactions governing later phases of trophoblast invasion into the endometrial stroma Mol. Reprod. Dev. 57:135–145, 2000. © 2000 Wiley‐Liss, Inc.