{'fraction_non_alphanumeric': 0.053303720155469185, 'fraction_numerical': 0.05719044975013881, 'mean_word_length': 5.0673400673400675, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Creatine supplementation attenuates inflammation in collateral lung after left lung transplantation

Background: Creatine can reduce oxidative stress and improve mitochondrial function, by improving protein turnover and mitochondrial energy production. Our hypothesis was that creatine (Cr) could attenuate ischemia and reperfusion injury (IRI). Objective: To evaluate the Cr role in the attenuation of IRI in collateral lungs of rats underwent to left lung transplantation (LTx). Methods: Sixty Sprague Dawley rats distributed into four groups: W90, control/water + 90 min of ischemia; Cr90, creatine + 90 min of ischemia; W180, control/water + 180 min of ischemia; Cr180, creatine + 180 min of ischemia. Donor animals received creatine (0.5g/kg/day) five days prior to LTx. Control group received only the vehicle. After 90 or 180 min of cold ischemia left lung was implanted and reperfused for 120 min. After reperfusion and isolation, we evaluated the right lung by inflammatory responses in lung parenchyma and BALF. Results: Edema and apoptosis in lung parenchyma decreased in both Cr groups (p≤0.001 and p≤0.007, respectively); TLR-7 was improved in Cr90 group; mononuclear cells were reduced in Cr90 and Cr180 groups (p≤0.017), but only Cr90 group showed a decrease in polymorphonuclear cells (p=0.001). The number of leucocytes and neutrophils in BALF was lower in both Cr groups (p≤0.028 and p≤0.011, respectively), and there was a decrease in macrophages in Cr180 group (p=0.019). Inflammatory mediators were reduced in Cr groups (IL6, p≤0.041; TNF alfa, p≤0.040; and CINC1, p≤0.002), and IL10 was increased in Cr90 group (p=0.003). Conclusion: Creatine supplementation attenuated the IRI in collateral lungs after left lung transplantation. Financial Support: FAPESP 2013/14848-1 and CAPES