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Simplified laboratory technique for diagnosis of chancroid.

Presumptive microscopic identification of Haemophilus ducreyi can be made from clinical material obtained from a lesion. By employing 10 ml of patient's blood (inactivated at 56 C for 30 minutes) as a cultural medium and serous fluid from the lesion as an inoculum, confirmation may be completed within 48 hours. A total of 24 patients with presumptive diagnosis of chancroid were examined and 21 of these showed H ducreyi on smears and culture.