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In vivo inhibition of Maillard protein fluorescence by acetylarginine: possible multiplicity of mechanisms

Medicinal plants are believed to be an important source of new chemical substances with potential therapeutic effects. There are reports concerning the antinociceptive effects of the plant Trigonella foenum-graecum (Fenugreek) in Iranian traditional medicine. These reports and hypoglycemic effect of fenugreek leaves extract encouraged us to assay cytotoxicity of fenugreek aqueous extract on the NIH3T3 mouse fibroblast cells. The cell line was purchased from National Research Center for Genetic Engineering and Biotechnology of Iran. The cells were plated in 24 well microtitre plates with DMEM + F12 medium containing 10% fetal calf serum supplemented with 445 mg/L L-glutamine and maintained at 37°C with 5% C02/95% air. Following 24 hrs incubation period, treatment was commenced by addition of various concentrations of the extract (0.01-ZO mg) to the culture wells, and the cells viability were assessed using Trypan blue and MTT assays following 5 days of incubation. The results show that IC50 of the fenugreek extract as calculated from the Trypan blue and MTT assays were 0.35 and 0.4 mg/ml, respectively.