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Lipid peroxidation products inhibit Glutathione based protection of Oxymyoglobin; a Model system

This study was performed as an in vitro model system to investigate the antioxidant role of glutathione in protecting oxymyoglobin from oxidation in competition with its role in detoxifying intracellular toxins such as 4-Hydroxynonenal (HNE). Briefly, the model consisted of a control solution (0.15mM oxymyoglobin solution), a positive control (0.2mM reduced glutathione (GSH) and glutathione peroxidase activity) and test samples (added HNE at 0.1, 0.2 and 0.4, 0.6 and 0.8mM). All samples were incubated for 300 minutes at pH 5.6 and 25 o C. Rapid oxidation of oxymyoglobin occurred in the control solution, while in the positive control, strong antioxidant activity was observed over the entire incubation period. Antioxidant activity was observed up to 240 mins in the presence of 0.1mM HNE, while in the presence of 0.2-0.6mM HNE antioxidant activity was observed for no more than 60 mins before the GSH antioxidant activity was apparently inactivated. A complete loss was observed at HNE concentrations of 0.8mM and above. At molar ratios of HNE to GSH greater than 1:1 the antioxidant activity of GSH seems to be inhibited. The level of HNE in meat are likely to exceed this ratio, thus it seems doubtful that GSH would be an important antioxidant in post mortem muscle.