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The UK National External Quality Assessment Scheme in Blood Group Serology. Compatibility testing 1983-1984: the influence of variables in test procedures on detection of incomplete antibodies.

Error rates in exercises in compatibility testing varied between 3% and 36% over the six years 1979-1984. Evolution of serological procedures was continuous through this period but without clear evidence of improvement in performance of antibody detection although performance in the UK appears to be comparable with that elsewhere. Relationships have been established between a number of variables of reagents and techniques, and their performance. The influence of some variables is reasonably clear but there appear to be interactions between certain variables in their effects on performance, and interpretation is less straightforward. A test for agglutination of enzyme treated cells together with an antiglobulin test appears to be the most reliable combination for detection of incomplete antibodies in compatibility testing although one stage enzyme techniques are an inevitable compromise between reliability and convenience. Compatibility testing should not be considered in isolation from antibody screening in determining the optimum combination of tests for pretransfusion antibody detection.