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DEVELOPMENT OF A BIOMIMETIC ENZYME-LINKED IMMUNOSORBENT ASSAY METHOD BASED ON A HYDROPHILIC MOLECULARLY IMPRINTED POLYMER FILM FOR DETERMINATION OF OLAQUINDOX IN CHICK FEED SAMPLES

In this study, a new way to substitute the biology antibody was introduced by using a hydrophilic molecularly imprinted film, which was directly prepared on the well surface of MaxiSorp polystyrene 96-well plate by the bulk polymerization technique. This imprinted film exhibited good recognition and fast adsorption-desorption dynamics toward olaquindox. Using it as the recognition element, a fast and new direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) method for the determination of olaquindox in chick feed was developed. This BELISA method had low cross-reactivities of 6.2% and 12% for two analogues. Under the optimal conditions, the sensitivity (IC50) and the limit of detection (IC15) were 700 ± 60 µg L−1 and 17.0 ± 1.6 µg L−1, respectively. The blank chick feed samples spiked with olaquindox at three levels were determined by this developed method with recoveries ranging from 82.0–96.0%.