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Separation and determination synchronously by multichannel mode‐filtered light capillary electrochromatography

Mode‐filtered light capillary electrophoresis (CE) was developed for capillary electrochromatography (CEC) by applying annular columns of different size (250, 320, and 530 μm) and a bovine serum albumin (BSA)‐modified chiral stationary phase. BSA was encapsulated on the pretreated surface of an optic fiber and the inner wall of a capillary using sol‐gel technique with a resultant larger active stationary phase area. The coating was examined by atomic force microscopy (AFM) and Fourier transform infrared (FT‐IR). The separation of DL‐tryptophan (10 mM) was investigated under different voltage/current conditions.