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Parameter Estimating Procedures for the Michaelis-- Menten Model : Reply to Tseng and Hsu

In a recent account of methods of obtaining best-fit parameters of the parameters V and Km of the Michael is-Menten equation, Tseng & Hsu (1990) appear to have misunderstood the method used by Lineweaver & Burk (1934), and to have overlooked much of the work done since 1934. The Michaelis-Menten equation represents the observed rate of v of an enzyme-catalysed reaction in terms of the two parameters V and Kin, and the substrate concentration s,