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Method for Measurement of Resistance of Oxidation in Beer by Electron Paramagnetic Resonance

This is the second year for this subcommittee’s evaluation of a method for the measurement of resistance of free-radical oxidation in beer by electron paramagnetic resonance (EPR). The term EPR is frequently used interchangeably with electron spin resonance (ESR). In the first year, the method produced unacceptable repeatability coefficients of variation and unacceptable reproducibility coefficients of variation (4). The subcommittee recommended that the collaborative study be repeated, incorporating changes such as freezing samples before shipping, sending the N-tert-butyl-αphenylnitrone (PBN) reagent as a solid with instructions on making up the standard (rather than sending a premade standard solution, which for many collaborators leaked during shipping), and sending samples with predetermined lag times.