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The effects of Jatropha curcas latex on activation and phagocytotic binding of Saccharomyces cerevisiae in RAW 264.7 macrophages

The latex derived from the subtropical and tropical plant Jatropha curcas (Euphorbiaceae) is commonly used throughout the world for the treatment of dermal abrasions. There is meager data as to the cellular mechanism of action responsible for the observed latex wound healing properties. In this study, we examined the effects of latex on activation of macrophages and the subsequent binding / recognition of Saccharomyces cerevisiae as a model to ascertain potentiating effects for macrophages to bind invasive pathogens. In this study, non‐viable yeasts destroyed by heat and peroxide (3.5x107/ml) were incubated with varying doses of latex in the presence of macrophages. The data indicate that macrophages incubated with latex (1.2 and 1.5mg/ml) for 15 minutes resulted in greater surface binding of yeast (186% and 191%) relative to untreated controls. The data also show that macrophages were not activated by the presence of latex, as assessed by quantification of inducible nitric oxide synthase and nitric oxide after 24 H incubation. In summary, the preliminary findings suggest that the effects of latex may involve greater trafficking of potential pathogens to immune cells, rather than stimulating the expression of pro‐inflammatory proteins. (Supported by a grant from the National Institutes of Health (NIH)/National Centers for Research Resources (NCRR: RR 03020)