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Nanobiocatalysts: Cu/TiO2-SiO2 Nanoparticles as Tissue-Regeneration Treatment for Diabetic Foot Ulcers: In Vivo Studies



Diabetic foot ulcers (DFUs) exhibit 80% of prevalence in Mexico. Efficient
tissue regeneration therapies are the key factors to avoid amputations.



In this study, the healing properties of a Cu/TiO2-SiO2 nanobiocatalyst applied in DFUs
were analyzed. Furthermore, the morphology and crystalline structures were characterized.



The nanobiocatalyst was synthesized by a sol-gel patented method proposed by López et
al. The compound was characterized by scanning and transmission electron microscopies and Xray
diffraction. The nanoparticles were embedded in a polymeric gel matrix (nanogel), which was
utilized as a conservative therapy for chronic non-healing DFU in 8 patients with several comorbidities
and chronic complications of diabetes. Wound debridement was performed prior to the
nanogel administration. The nanogel was applied over the ulcers on alternate days for different periods
of time, depending on the case.



Significant improvement in terms of enhanced epithelialization was observed in the
wound healing process after a few applications. Infection spread was limited, and tissue regeneration
was enhanced, with significant healing of the ulcers observed in each case. Furthermore, the
successful outcome allowed to avoid the amputations that were proposed to some of the patients.



The study proved the efficiency of the nanobiocatalyst as a safe, conservative therapy
for chronic non-healing DFUs. Further investigation must be carried out to fully elucidate the
wound-healing mechanisms of the nanoparticles.
