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Optimization of the HPLC analysis of biogenic amines in Peucedanum palustre plants and cell culture lines.

The HPLC separation of dansylated amides including mono-, di-, and polyamines from a Peucedanum palustre (L.) Moench plant and an embryogenic cell culture line was studied. The flowers, stems, leaves, and roots of the plant were analyzed separately. The mobile phase was optimized with the "PRISMA" model, which is a multi-solvent optimization system. Twenty-five standard amines were tested, twelve of which were present in the plant samples. The amine concentrations varied considerably in the different organs of the plant. There were also differences in amine concentrations between the wild plant and the embryogenic cell line and plantlets. The "PRISMA" optimization system proved to be a rapid and efficient method in the HPLC analysis of biogenic amines present in plant samples.