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Role of Adherence vs. Spreading in the Induction of Membrane‐Associated Interleukin‐1 on Mouse Peritoneal Macrophages

Adherence of peritoneal exudate cells (PEC) to plastic has been shown to activate several PEC functions, such as tumor ceil lysis and membrane‐associated interleukin‐1 (mlL‐1) expression. Several studies have demonstrated that leukocyte adherence is dependent on divalent cations. In this study, ethylenediaminetetrascetic acid (EDTA), a known chelator of divalent cations, was used to evaluate the role of cell attachment vs. spreading in adherence‐induced mlL‐1 activity on resident C57BL/6 mouse PEC. Significant inhibition of PEC spreading on plastic and mlL‐1 expression was noted when PEC were cultured in the presence of 10 mM EDTA. However, PEC remained adherent in the presence of EDTA and were able to express mlL‐1 activity in response to a soluble stimulus lipopolysaccharide (LPS) 1 μg/ml. These results suggest that the divalent cation‐dependent spreading of PEC on plastic initiates or enhances the expression of mlL‐1 activity. Additionally, adhesion and LPS stimulate mlL‐1 expression by independent mechanisms.