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The Impact of OCA2 (rs1800407) and HERC2 (rs12913832) Gene Polymorphisms on Iris Color in a Sample of Iraqi People

The study was directed in order to determine Single Nucleotide Polymorphisms (SNPs) ( rs1800407 G>A ) of OCA2 and ( rs12913832 A>G ) HERC2 genes and their association as a DNA marker with eye color in 60 samples of Iraqi people. Samples were collected from College of Science / University of Baghdad and also people from different areas of Baghdad, Iraq; during the two months period from September to November 2020. These SNPs determination was carried out by using quantitative real-time polymerase chain reaction (qPCR) of blood samples. The results of rs12913832 genotype showed significantly differences ( p<0.05 ) in the green eye color group. A Survey of HERC2 gene SNP genotypes and allele frequencies in the black and green iris groups showed that there was a significant variation in the wild type ( AA ) and homozygous mutant ( GG ) genotype frequencies in ( rs 12913832 ), while the SNP ( rs1800407 ) of OCA2 concluded that gene may have a non-significant effect among a sample of this study comparing with green eye to black eye color.