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Laser polarimeter LP101M and its applications in liquid cromatography

A high sensitivity laser polarimeter LP101M employing a He-Ne laser has been designed and constructed as a detector for liquid chromatography achieving a sensitivity better than 0.001 degree. The operation principle and technical characteristics of this instrument are described. A liquid gel chromatography column system suitable for sugar cane juice analysis was also designed and calibrated. It separated and analyzed the medium molecular weight carbohydrates and demonstrated the strong influence of these substances in the conventional polarimetric determinations.