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Effect of Bacterial Inoculants on Agronomic Performance of Three Genotypes of Potato (Solanum tuberosum L.) in Coastal Areas of Peru

Corresponding Author: Sergio Eduardo Contreras-Liza Universidad Nacional Agraria La Molina. Lima-Perú, Peru Tel: +511971759585 Email: scontreras@unjfsc.edu.pe Abstract: To study the effect of interaction of plant genotypes and populations of microorganisms in the rhizosphere may be an important factor to assess the effectiveness of PGPRs on crop production. The objective of this research was to determine the effect of inoculation of four bacterial strains on the agronomic performance of three commercial cultivars of potato in the province of Cañete, Peru. A randomized complete blocks design with three replications on a split plot arrangement was used, in which potato genotypes (three commercial varieties) were assigned to complete plots and inoculation treatments (bacterial strains and control) were allocated to sub-plots, then evaluating the effect for growth promotion related to bacterial strains: Bacillus amyloliquefaciens, B. simplex, Azotobacter sp. and Pantoea sp. on potato agronomic characteristics. As result of inoculation with bacterial strains in potato cultivars Canchán, Perricholi and Unica, significant differences were showed (p<0.05) for vegetative vigor, percentage of inflorescences per plant, foliage weight per plant and weight of the total biomass per hectare with use of inoculation of some of these strains, having a stimulatory effect on development in these commercial varieties, highlighting strain AZO16 M2 (Azotobacter sp.) to promote increased weight of foliage and total biomass and flowering in cv. Perricholi and strain Bac15MB (Bacillus amyloliquefaciens) to regulate the degree of senescence of foliage, specifically in cv. Canchan. Concluding that inoculation with bacterial strains in potato cultivars Canchan, Perricholi and Unica influenced some important crop agronomic characters, although its specific effect was dependent on each potato variety.


Introduction
In the decade of 1960-2000, potato production in Peru increased from 1.3 to 3 million tons per year according to Maldonado et al. (2008). This volume of production has made the country's largest potato producer in Latin America. On the other hand, it is known that the potato crop is very sensitive to a number of biotic and abiotic environmental stresses; potato diseases can greatly reduce productivity and tuber quality (FAO, 2008). These problems, coupled with the fact that potato is propagated by vegetative means, make crop health as a considerable problem for the production of quality planting material. The virtues of potatoes, particularly its high nutritional value and their ability to increase farmers' income, have not been the attention they deserve from governments (FAO, 2008), so it is necessary to invest in new technologies with potential to reduce the risk of the crop.
Many bacterial strains with important biological activity, belonging to the genera Azoarcus, Azospirillum, Azotobacter, Arthrobacter, Bacillus, Clostridium, Enterobacter, Gluconacetobacter, Pseudomonas and Serratia, have been reported (Maheshwari, 2011); including importantly the species Pseudomonas sp. and Bacillus sp. as the most extensively studied. Ecological factors such as temperature and nutrient soil conditions affect the behavior of rhizobacteria; bacterial inoculation has a better stimulatory effect of plant growth under conditions of nutrient-deficient soils or rich fertile soils (Egamberdieva, 2012).
Under controlled conditions Bacillus sp. produce clues to the underlying mechanisms of better growth of potato plants as early tuberization, rapid development of leaf area and probably higher rate of photosynthesis (Oswald et al., 2010). Calvo et al. (2008) observed an effect of potato variety on populations of microorganisms in the rhizosphere, confirming the influence of plant exudates on the rhizosphere populations.
Some beneficial microorganisms such as promoting rhizobacteria plant growth and mycorrhizal fungi, may have mediated the effect of plant phytophagous insects; the plant growth promoting rhizobacteria Pseudomonas fluorescens can induce systemic resistance in Arabidopsis thaliana against several microbial pathogens and chewing insects (Pineda et al., 2012).
High prevalence of antagonistic microorganisms was found in bacterial native strains for soil pathogen Rhizoctonia solani; even more, these strains inhibited the growth of Fusarium solani, showed a certain level of auxin indole-3-acetic acid and participate in the solubilization of tri-calcium phosphate. Phylogenetic analysis revealed that most strains belonging to species B. amyloliquefaciens, while strains Bac17M11, Bac20M2 and Bac20M1, as a new group of putative Bacillus sp. (Calvo and Zúñiga, 2010).
The ability of plant growth promotion in strains of Azotobacter sp. and actinomycetes isolated from potato fields in the Andean highlands of Peru, was evaluated by Rico (2009). He found that 42.3% of strains inhibited the growth of Fusarium solani and 17% of Rhizoctonia solani; also that 56.5% of Azotobacter strains produced indole acetic acid (auxin) and 46.7% produced phosphate solubilization halos. Most Azotobacter isolates were recognized as A. chroococcum and A. vinelandii. At greenhouse conditions, Azotobacter strains showed promotion of plant growth in root crops.
The aim of this research was to determine the interactive effect of bacterial strains and commercial cultivars of potato on the agronomic performance in field conditions of the province of Cañete (Peru) and to propose alternatives to the use of external chemical inputs in potato cultivation, favoring the development of plant defenses through the application of biological inducers to increase and improve natural immunity and agronomic performance in farmer fields.

Materials
Seed-potato tubers from in vitro plantlets of cultivars "Unica" CIP number 392797.22 (Gutiérrez-Rosales et al., 2007), "Perricholi" CIP number 374080.5 and "Canchan" CIP number 380389.1 (CIP, 2013) were provided by International Potato Center (CIP Genebank, Lima) and selected considering their vigor and emergence of sprouts, verifying that were free of pests or diseases.
The biological material (bacterial strains) was provided by Laboratorio de Ecología Microbiana y Biotecnología Marino Tabusso at Universidad Nacional Agraria La Molina (Lima). For the purpose of the experiment, inoculation was carried out with the following bacterial strains isolated from the rhizosphere of potato (Calvo and Zúñiga, 2010): • Bacillus amyloliquefaciens strain Bac15MB • Bacillus simplex strain B13 • Azotobacter sp. strain AZO16M2 • Pantoea sp. strain DZ22

Experimental Site
Field research was conducted during the spring season at district Quilmaná, located at province of Cañete, between the geographical coordinates 12°57 '34''S and 76º23'17''W, on average height 151 masl. The weather during the trial was characterized by average temperatures between 16 and 24°C, relative humidity of 82-84% and 2 mm. for monthly rainfall. The soil had the following characteristics: organic matter 0.76%, CaCO 3 0.93%, electrical conductivity 3.40 dS.m −1 , pH 7.84, 16.68 ppm available phosphorus, 154 ppm available potassium, cation exchange capacity 7.83 meq per 100 g.

Preparation and Application of Treatments
In a conditioned area were measured the amounts of each bacterial strain used as separated treatments for each potato cultivar. A dose of 30 ml pure bacterial inoculum (density of ca. 10 8 cfu.mL −1 ) was used for each treatment and additionally four liters of deionized water to make the immersion of the whole planting material and being impregnated bacterial inoculants in seed tubers. Then, immediately proceeded to the field planting at density of 30 000 plants per hectare. The control plots were applied at a dose of 0.4 mM acetylsalicylic acid (pharmacological use) in immersion to the tubers; later 15 days after emergence of the plants, the same dose was applied spraying the foliage. In all cases, seed tubers inoculation lasted 5 min, after which they were sown in field plots. For this, a sample of approximately 200 potato tubers were subdivided into 5 batches of 40 tubers for inoculation treatment of each bacterial strain and a control in each repetition. Inoculation treatments with bacterial strains were again applied to plant foliage at 45 days after planting, at a dose of 30 ml. pure inoculum (density of ca. 10 8 cfu.mL −1 ) with additional 10L current water to each of the four bacterial strains; control treatment (with no bacterial inoculants) were watered on plant foliage.
A single dose of vermicompost enriched with phosphate rock was applied to the soil at potato planting, equivalent to 7.40 t. ha −1 . In vermicompost, an average content of 1% N, 2.5% and 0.5% K 2 O P 2 0 5 (Pineda, 1999), equivalent as dose per hectare of 74 kg nitrogen, 185 kg P 2 0 5 and 37 kg K 2 O. During cropping period, no fungicides were applied and chemical control for pests was applied against leafminer fly (Liryomiza huidobrensis) with Abamectina and Cyromazine.

Experimental Design and Statistical Analysis
A completely randomized design was used with 3 replications on a hierarchical arrangement of split plots (Steel, Torrie & Dickie, 1997) in which potato genotypes (3 cultivars) were assigned to whole plots and 5 treatments of inoculation (4 bacterial strains + control), were allocated to sub-plots. Both plots and sub-plots were randomized in blocks. Analysis of variance was performed and Tukey test was used with a significance level α = 5%. For systematization of information, a spreadsheet (Excel) was used and InfoStat Software (Balzarini et al., 2014) for statistical processing of field data.

Traits Evaluated
The variables for field evaluation were: number of sprouts per plant at 15 days after planting, density of plants per plot emerged at 30 days after sowing, vegetative vigor measured as a phenotypic scale of 1 to 9, plant height 60 days after planting, percentage of plants with visual symptoms of virus infection (data transformed to square root), percentage of damaged plants with leaf miner fly (Liriomyza huidobrensis) at 60 days, percentage of affected plants with Rhizoctonia solani 60 days after sowing (square root transformed data), percentage of flowering 75 days after sowing (square root transformed data). Also was evaluated: degree of senescence of foliage at 100 days measured in phenotypic scale from 1 to 9, fresh weight of foliage at harvest, number of tubers per plot at harvest, weight of tubers per plot at harvest, general appearance of the crop in phenotypic scale from 1 to 9, average weight of tubers per plant, tuber yield projected for 30 000 plants.ha −1 , total weight of biomass (tuber + foliage) projected per hectare. Table 1 and 2 show the results of the analysis of variance for agronomic and productive traits in commercial potato cultivars Canchan, Perricholi and Unica under effect of inoculation with bacterial strains. This analysis shows that all evaluated characters show variation mainly for potato genotypes as expected. Also, there can be noted significant variation for main effects of inoculation with bacterial strains with respect to foliage weight per plot and total weight of biomass per hectare. Other agronomical traits influenced significantly by bacterial inoculants were vegetative vigor and flowering percentage; in the case of interactions of potato genotypes and bacterial strains, there were significant variations for vigor vegetative at 60 days, percentage of flowering at 75 days, foliage weight per plot and total weight of biomass at harvest.

Results
No significant changes were found for sources of inoculation with the bacterial strains or its interactions with potato genotypes with regard to yield of tubers per hectare. Regarding vegetative vigor (Fig. 1) it was showed a significant interaction between potato genotypes and bacterial strains so there was differential response to inoculation with bacterial strains in genotypes of potato, mainly in cv. Unica. In this potato cultivar was observed that control had the lowest value of vigor in relation to bacterial treatments, except for strain DZ22 (Pantoea sp.), in which there were no statistical differences.
In Figure 2 it can be seen that for cv. Perricholi some bacterial strains (AZO16M2, B13 and Bac 15MB) showed a significantly higher level of inflorescences compared to control and strain DZ22. In particular, bacterial strain AZO16M2 (Azotobacter sp.) in potato cv. Perricholi presented a greater effect in relation to percentage of inflorescences compared to control. Canchan and Unica potato cultivars showed no significant flowering.   In Figure 3 is noted that degree of senescence of the foliage was not significant in potato cv. Unica and Perricholi. In cv. Canchán bacterial strains as a whole and in particular strain Bac15MB (Bacillus amyloliquefaciens.) had a slight effect in relation to control, to regulate the degree of senescence of foliage at 105 days of planting.
In Figure 4 we can see that there were significant effects in foliage fresh weight per plant for cv. Perricholi; in this case, strain AZO16M2 (Azotobacter sp.) had significant effects compared to the control and strain DZ22 (Pantoea sp.), obtaining the highest weight value foliage per plant. In potato cv. Canchan and Unica there were no significance.
For tuber yield per hectare, there were no statistical differences between genotypes and bacterial strains within each variety; however, it could be noticed some simple effects, specifically shown by the strain DZ 22 (Pantoea sp.) in cv. Perricholi which reached the highest yield of tubers per hectare (20.84 t) in relation to other bacterial strains but this effect was not consistent in other varieties tested, as shown in Fig. 5. In relation to fresh weight of biomass per hectare can be seen in Figure 6 that there were statistical differences just for cv. Perricholi in which the effect of inoculation with bacterial strain AZO16M2 (Azotobacter sp.), was statistically superior to control and treatment of DZ22 strain; in cv. Unica and Canchan there were no differences for inoculation of any rhizobacteria with respect to weight of biomass.  Interactions between potato genotypes and bacterial strains can be showed in Tables 3 and 4. No significant interactions were found for most traits evaluated, except for plant vigor and flowering percentage. There were also interactions for some production traits as plant senescence and biomass for cv. Canchan and Perricholi, respectively.

Discussion
Inoculation of seed tubers of potato cultivars Canchan, Perricholi and Unica, significantly influenced the following characters: vegetative vigor, percentage of inflorescences, weight of foliage per plot and weight of the total biomass per hectare, in which significant interactions between varieties of potato and bacterial strains also were presented. This is consistent with the findings by Calvo and Zúñiga (2010) who observed an effect of potato variety on populations of microorganisms in the rhizosphere, which may explain the interaction effects between potato genotypes and bacterial strains in some characters, such as we found in this investigation.
No significant changes showed in the sources of inoculation with the bacterial strains or its interactions with potato genotypes with regard to yield of tubers per hectare or some of its components, but significantly, there were some specific effects of inoculation of bacterial strains on potato genotypes. In this regard, Oswald et al. (2010) claim that three rhizobacteria of the taxa Bacillus, Actinomycete and Azotobacter increased tuber yield significantly, comparable with the yields of different varieties of potato with inorganic fertilization, under greenhouse and field conditions in Peru, results that agree partially with findings in this investigation, although growing conditions and the varieties used in both cases, were different.
Relative to the total weight of the biomass was observed only statistical differences for cv. Perricholi, in which the effect of inoculation with the bacterial strain AZO16M2 (Azotobacter sp.), was statistically superior to control; Rico (2009) reported similar results with Azotobacter strains showed plant growth promotion, potato tuber production and solubilization of phosphates.

Conclusion
Some concluding remarks can be suggested from the present research for agronomical response of potato cultivars inoculated by bacterial strains: • Vigor: there was a significant interactive effect for vegetative vigor between potato genotypes and bacterial strains and a differential response to inoculation with bacterial strain AZO16M2 (Azotobacter sp.) in cv. Unica • Flowering: tuber seed inoculated with bacterial strain AZO16M2 (Azotobacter sp.) in potato cv. Perricholi showed a greater effect in flowering percentage • Senescence: In cv. Canchan bacterial strains as a whole, and in particular strain Bac15MB (Bacillus amyloliquefaciens) had a slight effect in regulating senescence compared to control • Foliage fresh weight: significant effects for cv. Perricholi, in this case for strain AZO16M2 (Azotobacter sp.) • Total Biomass per hectare: For cv. Perricholi the effect of inoculation with bacterial strain AZO16M2 (Azotobacter sp.) was significant It can be concluded that the inoculation with bacterial strains studied in potato genotypes Canchán, Perricholi and Unica, can be an appropriate technology to promote potato growth in important agronomic characters, although its specific effect is dependent on each genotype, so that may be recommended some bacterial strains for use as inoculation treatment having a stimulatory effect for development in these commercial varieties.