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Detection of Cystathionine Ketimine in Bovine Cerebellum

Abstract: A new sulfur‐containing cyclic imino acid, cystathionine ketimine, has been detected in bovine cerebellum by gas chromatography, gas chromatography‐mass spectrometry, and high pressure liquid chromatography procedures. Gas chromatography and gas‐mass analyses are based on derivatization of endogenous cystathionine ketimine with diazomethane after a simple enrichment procedure. The high pressure liquid chromatography procedure takes advantage of the selective absorbance at 380 nm of the phenyl isothio‐cyanate‐ketimine interaction product. The concentration of this new sulfur imino acid found in a pool of four bovine cerebella is ∼0.5 nmol/g.