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Quantification of sweat urea in diabetes using electro-optical technique.

Diabetic kidney disease is one result of prolonged elevation in blood glucose level. When insulin secretion reduces, serum urea level increases and vice versa is also true. Hence monitoring urea level in blood is important in diabetic subjects. Any change in serum urea will have impact on sweat urea concentration. Attempted in this study is to develop an optical device for quantifying sweat urea concentration. It uses light sources, light sensors with time and intensity controlled operation and suitable calibration algorithm. Sweat samples are collected from group of volunteers belonging to control and diabetes. After sedimentation and suitable pre-processing, sweat samples are irradiated by primary colour light sources operated sequentially. Reflected light intensity is used to compute the sweat urea concentration. Obtained results when compared with standard lab techniques like UV-visible absorption spectroscopy and colorimeter, correlation of 98% with error less than 3% is achieved. Results also demonstrate elevation in sweat urea level with years of diabetes, in spite of serum urea level being within limits. We extended the study on kidney disease subject and observed the influence of blood glucose on urea. Therefore the proposed device can be used to measure sweat urea periodically so that any change can be observed at an early stage and diabetic nephropathy could be prevented at large.