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Teasterone-3-O-β-D-glucopyranoside, A New Conjugated Brassinosteroid Metabolite from Lily Cell Suspension Cultures and Its Identification in Lily Anthers

The new brassinosteroid conjugate, teasterone-3-O-β-D-glucopyranoside, was found as a metabolite of teasterone in lily cell suspension cultures. Its structure was determined by means of FAB-MS and 1H-NMR upon comparison with the authentic compound. Furthermore, its presence in lily anthers was confirmed by FAB-MS and LC-APCI-SIM data. This is the first natural brassinosteroid conjugate glucosylated at a hydroxyl group in ring A.