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[Changes, during a synchronous cell cycle, of the level of incorporation of 5-bromodeoxyuridine into DNA of chick embryo fibroblasts].

The determination of the buoyant density of synchronously dividing Chick embryo fibroblast DNA, substituted at various times during the cell cycle by 5-bromodeoxyuridine (BrdU), has been carried out by means of analytical neutral CsCl banding. It has been shown that the buoyant density of BrdU-substituted DNA, duplicated during the first half of S phase, was 0.010 g/ml higher than that of BrdU-substituted DNA duplicated during the second half of S phase. That may reflect a preferential incorporation of the analogue at the beginning of S phase. This peculiar pattern of incorporation would be responsible for the observed inhibition of cell transformation occurring when fibroblasts, infected by Rous sarcoma virus, are grown in the presence of the analogue.