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The inhibitory effect of sinigrin on the production of inflammatory mediators induced by lipopolysaccharide in RAW 264.7 macrophages (1056.5)

Sinigrin (2‐propenylglucosinolate) is a biochemical nutrient found naturally in some plants of the Brassicaceae family such as broccoli, Brussels sprouts. Recent studies showed that sinigrin affect the preventing of cancer in preclinical or clinical trials for cancer chemoprevention. However, anti‐inflammatory effect of sinigrin was unknown. In the present study, we examined the effects of sinigrin on the production of inflammatory mediators in lipopolysaccharide (LPS)‐activated RAW 264.7 macrophages. The cell viability was determined by MTT assay. We investigated the anti‐inflammatory effects of sinigrin on Nitrite Oxide (NO) and pro‐inflammatory cytokine production by colorimetric assay and ELISA assay, respectively. We also examined the expression of MAPK, NLRP‐3 and p65 by Western blot. The results showed that NO production was not reduced by sinigirn.