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3H‐URIDINE INCORPORATION BY SMALL LYMPHOCYTES Effect of Antilymphocyte Serum, Cyclophosphamide, and Irradiation

SUMMARY The present study demonstrates that with the possible exception of the lymph node, the small lymphocytes of the lymphoid tissues of normal rats cannot be distinguished on the basis of 3H-uridine incorporation. In contrast, after treatment with antilymphocyte serum, thoracic duct small lymphocytes incorporated less 3H-uridine than lymphocytes from the same compartment of untreated animals. This depression is most likely not attributed entirely to a shift in small lymphocyte populations but may result, in part, from an effect of antilymphocyte serum on individual cells. Surviving cells from cyclophosphamide or total body irradiated rats did not evidence this shift in the 3H-uridine incorporation.