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miR‐188 promotes senescence of lineage‐negative bone marrow cells by targeting MAP3K3 expression

Lineage‐negative bone marrow cells (lin‐BMCs) have reparative potential for overcoming endothelial dysfunction and reducing cardiovascular risk. Here, we found that miR‐188 is upregulated and mitogen‐activated protein kinase kinase kinase 3 (MAP3K3) is downregulated in aged lin‐BMCs, whereas their expression is reversed in young lin‐BMCs. We identified and confirmed MAP3K3 as a direct target of miR‐188. MiR‐188 overexpression or MAP3K3 silencing in young lin‐BMCs increases p16 and p21 expression, enhances cell senescence, and decreases the ability for cell proliferation, migration, and tube formation. Conversely, miR‐188 suppression in aged lin‐BMCs yields the opposite results. We further found that MAP3K3 is involved in miR‐188–induced promotion of lin‐BMC senescence. All data reveal that miR‐188 induces lin‐BMC senescence by targeting MAP3K3 expression, thus, providing new theoretical basis for the prevention and treatment of cardiovascular diseases.