{'fraction_non_alphanumeric': 0.027761711972238288, 'fraction_numerical': 0.006940427993059572, 'mean_word_length': 5.67953667953668, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Changes in the Intestinal Microbes of Wild Spermophilus dauricus (Rodentia: Sciuridae) in Different Periods of Hibernation (A Model of Gut Ischemic Reperfusion Injury)

Reperfusion injury after intestinal ischemia and hypoxia is a complex and comprehensive disease with a poor prognosis and high fatality rate. However, whether it can promote or slow down intestinal ischemia-reperfusion injury remains unclear. Spermophilus dauricus is a hibernation animal with a short awake time that interrupts the long hibernation period. When these events occurred, the long-term ischemic intestine in hibernation rapidly congested and entered the hibernation and ischemic state again. However, Spermophilus dauricus is not sick, and this might be a good model for gut ischemic reperfusion injury. In this animal research study, 16s rRNA gene sequencing was used to detect the bacterial community composition of S. dauricus intestine before, during, and after the artificial hibernation process and short awakening hibernation, respectively. The results showed that there was no significant alpha diversity during the four periods; however, the beta diversity was different, especially in the awakening period. These differences in beta diversity may be due to the significantly increased abundance of 11 kinds of bacteria, including Anoxybacillus, Geobacillus, Klebsiella, Thermus, and Acinetobacter ( P <0.05), and the significantly reduced abundance of 16 species of bacteria, including Acinetobacter, Aquabacterium, Acidovorax, Thermus, and Klebsiella ( P <0.05) in the awakening period. This study provides guidance and reference for the exploration of animal hibernation mechanisms and research on intestinal ischemic re-enema injury.