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Identification, Pathogenicity, and Fungicide Sensitivity of Colletotrichum Spaethianum Isolates, the Causal Agents of Anthracnose of Daylily in Shanghai, China


 Anthracnose caused by Colletotrichum spp. is a common disease of daylily (Hemerocallis fulva) and results in large economic losses. In this study, typical leaves suspected to be infected with anthracnose were collected, and two isolates were obtained by tissue separation combined with single spore separation. Based on the analysis of morphological characteristics, multi-gene phylogenetic analyses (ITS, GAPDH, ACT, and TUB2) and pathogenicity tests, both isolates were identified as Colletotrichum spaethianum. Pathogenicity tests showed that both isolates were able to cause typical daylily anthracnose symptoms. Fungicide susceptibility analyses showed that the two isolates were highly sensitive to fungicides, including zineb, difenoconazole, captan, and carbendazim, based on the inhibition of mycelial growth with EC50 values ranged from 0.0903 to 3.402 µg/mL. This is the first report of C. spaethianum causing daylily anthracnose in China. This study provided a comprehensive reference for the pathogen, symptoms and pesticide prevention of daylily anthracnose, which may help the sustainable management of daylily anthracnose.