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Thin-Layer Chromatographic Separation of Cadaverine and Ornithine, and Spectrophotometric Quantification

Biogenic polyamines are fundamentally important in regulation of cellular growth and differentiation [1]. Polyamines have been recognized as sensitive biomarkers for detection of cancer and for assessing the success of therapy [2–5]. Chromatographic methods, including high-performance liquid chromatography [6, 7], gas chromatography [8], capillary electrophoresis [9], and thin-layer chromatography (TLC) [5, 10, 11], have been reported for analysis of polyamines. Because of their high polarity and solubility in water, however, polyamines must be derivatized before chromatography to avoid severe tailing and to achieve a satisfactory separation. TLC is a simple and inexpensive technique and continues to be the method of choice in small laboratories of developing countries which cannot afford sophisticated instrumentation. We have previously shown that calcium sulfate (CaSO4) is an excellent adsorbent for separation of polar compounds by TLC [12, 13]. The objective of this investigation was to compare the chromatographic potential of CaSO4 and silica gel for quantitative separation of underivatized cadaverine and ornithine.