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Conformational analysis of an opioid peptide in solvent media that mimic cytoplasm viscosity

Many neuropeptides exert their action between the presynaptic vesicles and postsynaptic transmembrane receptors, crossing different layers of specialized cytoplasm. Biomimetic media usually employed to study bioactive peptides do not reproduce the physico chemical environment of cytoplasm—in particular, the high viscosity of this biological fluid. Here we describe a Conformational study of a δ‐selective opioid peptide, deltorphin I, at variable temperatures in several biocompatible media characterized by varying values of viscosity and dielectric constant. It was found that only viscosity, among these parameters, induces ordered conformations; that is, it acts as a Conformational sieve. This finding suggests that the high viscosity of the intersynaptic fluid contributes, in addition to the membrane catalysis proposed by Schwyzer, in overcoming the so‐called entropic barrier to the transition state of peptide–receptor interaction by selecting ordered conformations prior to receptor interaction.