{'fraction_non_alphanumeric': 0.03421727972626176, 'fraction_numerical': 0.00855431993156544, 'mean_word_length': 5.09375, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Analysis of Orphenadrine Citrate in Various Chiral Stationary Phases: A Comparative Study



Polysaccharide based chiral stationary phases (CSPs) were used to perform
enantiomeric separation of Orphenadrine Citrate by Ultra-Fast Liquid Chromatography (UFLC)
technique. Trials were conducted using the polar mode, reverse phase mode and normal phase mode.
Amylose and Cellulose-based CSPs were used for the same.



Eight Amylose-based CSPs and four Cellulose-based CSPs were used in
the reverse phase mode. Five Amylose-based CSPs and two Cellulose-based CSPs were used in polar
mode. The only Cellulose-based CSP used in the normal phase mode could effectively separate
Orphenadrine Citrate enantiomers with a good resolution.



Successful enantioseparation was obtained using Chiralcel OD-H containing
Cellulose tris (3, 5-dimethylphenylcarbamate) as a chiral selector and n-hexane: Ethanol: Diethylamine
(95: 05: 0.1, v/v/v) as the mobile phase. The developed method was validated in accordance
with ICH guidelines (Q2R1).



The proposed objectives were successfully accomplished as the developed method
could effectively resolve Orphenadrine Citrate enantiomers.
