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Detection of Phytoplasmas Associated with Tomato Witches’ Broom and Big Bud Disease in Egypt

Symptoms of witches’ broom were observed on tomato plants in some fields in Aswan governorate, Egypt. Molecular investigation was done with phytoplasma-detection primer P1/P7 to amplify a fragment of the 16S rRNA gene plus spacer region and beginning of 23Sr gene. RFLP analyses and sequencing of DNA fragments amplified by R16F2n/R2 primers indicated that these phytoplasmas are closely related and could be classified in the 16SrII group. Phylogenetic analyses of 41 accessions of 16S ribosomal RNA gene sequences of ‘Candidatus phytoplasmas’ comprising the strain from Egypt and representative strains from GenBank confirmed that the phytoplasma from tomato cluster with other strains all classified in subgroup 16SrII-D. Therefore, it could be useful to use the RFLP methodology in rapid and specific screenings of this phytoplasma presence in Egyptian tomato plants.