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[The influence of proteins bound to single-stranded DNA on RNA and poly(A) synthesis. I. Antibodies against DNA].

The influence of antibodies to DNA of T4 and SPOI phages as well as to DNA of E. coli on RNA and poly(A) synthesis by E. coli RNA polymerase has been studied. Antibodies to the phage DNA specifically inhibit RNA and poly(A) synthesis by 30 to 60% only in case of homologous DNA, the stage of elongation being inhibited predominantly. A comparison between the effects of the antibodies upon different stages of RNA synthesis on the native DNA and on the synthetic oligonucleotide template (dT)12 allows to draw a conclusion that upon inhibition of the synthesis the antibodies interact with a single-stranded site of the template not covered with RNA polymerase molecule, this site being formed during transcription of double-stranded DNA.