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Influence of anaerobic conditions on vaginal microbiota recovery from bacterial vaginosis patients

Bacterial vaginosis (BV) is one of the most common infections in women of reproductive age. Clinical studies have shown an association among BV and abnormal pregnancy, pelvic inflammatory disease and an increased risk of sexually transmitted infections, including HIV.1 This disorder was first described in 1914 by Curtis as a ‘white discharge’ syndrome2 and despite the decades of research we have only limited, and clearly not conclusive, evidence of microbial cause of BV, mechanism of disease and effective treatment.

The development of molecular techniques such as Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing produced a clearer picture of the …