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Characterization of four murine monoclonal antibodies recognizing respectively HLA-A2 plus Aw69, HLA-B13, HLA-Bw4 and HLA-A28.

Here reported is the characterization of four cytotoxic polymorphic anti-HLA class I monoclonal antibodies (MoAbs), specific respectively for HLA A2+w69,A28,B13, and Bw4, that can be used for standard HLA typing instead of the conventional alloantisera. MoAbs Wu1, Wu2, Wu3 and Wu4 were produced and primarily identified in China, in the Wuhan Institute of Biological Products. The hybridoma were constructed with the murine myeloma line NS-1 and splenocytes from Balb/c mice immunized with PBL from individual healthy donors