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Micellar electrokinetic capillary chromatography for simultaneous determination of five Sudan dyes in chili products

A method for simultaneous determination of Sudan red I ~ IV and G in chili products including chili powder,chili oil and chili sauce by micellar electrokinetic capillary chromatography was established. The samples were extracted with n-hexane,purified and enriched with neutral alumina column. The target dyes were eluted with 5% acetone-n-hexane,and dried-up under nitrogen flow. The residue was redissolved in acetonitrile and then diluted with 40 mmol / L borax- 40 mmol / L SDS( 1: 2,V / V) for analysis. The target dyes were separated with a capillary with the running buffer consisted of borax,sodium lauryl sulfate and acetonitrile( p H9. 2). Under the optimized conditions,the five azo-dyes were baseline separated within 20 min. The linear ranges of the method were 1 ~ 20 mg / L and the correlation coefficients were greater than 0. 997. The detection limits were 0. 12 ~ 0. 62 μg / m L and the quantification limits were 2. 4 ~ 12. 4 mg / kg. The recoveries were76. 3% ~ 98. 6% and the relative standard deviations( RSDs) were less than 5%.