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[Methanogenic activity and methanogen diversity in marine gas field sediments].

Methanogens play an important role in marine sediments, which are related to methane production and methane hydrate deposits. Methanogenic activity of marine gas field sediments was investigated using substrates that methanogens usually used as carbon sources. H2/CO2, methanol, methylamines and trimethylamines could support the growth and methane production of gas field sediments. 16S rRNA gene sequence analysis indicated that the predominant methanogens in the enrichment cultures were related to known cultured methanogens in the family Methanosarcinaceae of the order Methanosarcinales and the family Methanomicrobiales of the order Methanomicrobiales, with genera Methanococcoides, Methanogenium and Methanosarcina as major methanogens.