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Quantitation of oleuropein in virgin olive oil by ionspray mass spectrometry-selected reaction monitoring.

This work describes the unambiguous evaluation of the presence of oleuropein in virgin olive oils by ionspray tandem mass spectrometry (ISI-MS/MS). The oil samples obtained from different cultivars, such as Carolea, Cassanese, Coratina, Dolce di Rossano, Roggianella, and Tonda di Strongoli, grown in different geographical areas of Calabria, Italy, have shown an average content of oleuropein ranging from 1 ppb to 11 ppm. Commercial virgin oil samples, blended in some cases, contain significant amounts of this pharmacologically important antioxidant. The MS/MS methodology was applied in a triple-quadrupole instrument, through continuous scanning of the third analyzer to detect oleuropein in methanol extracts and in selected ion monitoring (SRM) for its quantitative assay.