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Inhibition of cell wall degrading cellulase enzyme – the incitant of anthracnose disease caused by Colletotrichum capsici on capsicum fruit

Abstract Cell wall degrading enzymes, namely cellulase, were detected in senescence and diseased macerated tissue of capsicum fruit. Their purported role in pathogenicity of Colletotrichum capsici was studied in detail. In situ production of cellulase concentration ranging from 80 – 360 μg was recorded in healthy capsicum fruit during storage. Production of this enzyme increased with an increase in the storage period. Increase in production of cellulase enzyme was recorded in infected tissue from seventh day after inoculation. Increased productions of this cell wall degrading enzyme coincide with disease manifestation and rapid progress of decay on capsicum fruit. Production of cellulase enzyme ranged from 80 – 360 μg. Conidial germination with advance of incubation period and further growth of C. capsici increased the production of cellulase enzyme. Conidia of C. capsici showed a gradual increase in production of cellulase enzyme with an increase in the incubation period. Treatment of capsicum fruit with different concentrations of calcium chloride, lactic acid and sorbic acid showed a 100%, 85.1% and 83.2% decrease in production of cellulase enzyme at the concentration of 1000 ppm, 500 ppm and 1000 ppm respectively.