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New Biosensor Using Shear Horizontal Surface Acoustic Wave Device

This paper describes a new biosensor to detect an enzyme reaction in liquid using surface acoustic wave (SAW) devices fabricated on 36°-rotated Y-cut, X-propagating LiTaO3. The sensing wave on the substrate is a predominantly shear-horizontal-mode SAW (SH-SAW) and is affected by a strong acoustoelectric interaction between the piezoelectric potential and electrical properties of the materials in the adjacent liquid. As an example of an electrical property, pH change associated with an enzyme reaction leads to measurable perturbation in the wave-propagation characteristic. Taking advantage of this phenomenon we realized a SAW biosensor which consists of an immobilized urease membrane on the surface. Also, highly sensitive detection for the urea solution was obtained in our preliminary experiments.