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A proposed method for the determination of the components of an oral contraceptive mixture (norethisterone acetate and ethinyl estradiol).

An assay for determining the mixture of norethindrone acetate (NA) and ethinyl estradiol (EE) is reported. The technique does not require separation of the compounds prior to analysis. The NA content is determined by a diphasic titrimetric method utilizing sodium dioctylsulphosuccinate titrant after reaction of the ketosteroid with hydroxylamine. The EE content is determined by the diazo coupling reaction utilizing the diazonium salt of sulphathiazole. The advantages of the technique over other techniques are delineated and the experimental procedure is described.