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The effects of bandpass intensity distribution spectrum on measurements of solid samples using a spectrofluorometer.

The monochromators used in spectrofluorometers typically behave as filters with a triangular-shaped intensity transmission spectrum, with the bandpass value referring to the width of the triangular spectrum at half the maximum intensity. The effects of the shape of the waveband upon the reflectance and fluorescence measurements made with the spectrofluorometer are discussed from a geometric point of view, particularly in relation to subsequent colorimetric calculations.