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Data representation and visualization in 4-D microscopy

Computer representation in biological microscopy is progressing from the well established modeling of three-dimensional (3-D) structural information towards the visualization of spatio- temporal (4-D) information. This paper describes two new methods to process sequential volumes, where each data set corresponds to a time sample. The first technique is based on surface rendering to study organ and tissue development. Contour stacks are rendered and in- between stages are interpolated. This technique allows the analysis and simulation of growth following different mathematical models and relates them with experimental findings. The second technique got appreciation for volume rendering of morphogenesis in living tissue. Sequences scanned with a confocal microscope are packed. The combination of ray-casting reconstructions within a color model allows for a rendering of morphogenetic activity.