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[Trypanosoma cruzi: differences in the requirement for protein protectors presented by 2 Argentinian strains].

The protein protector requirements for CA-I and RA T. cruzi trypomastigotes were standardized. The CA-I trypomastigotes needed a supplement of 5% serum or 5% bovine albumin in the washing and/or resuspending solutions to preserve their viability. For RA trypomastigotes, requirements were in the order of 1%. Trypomastigotes viability was determined by motility at the fresh microscopic observation and by mouse inoculation. This is a further demonstration of the particular behaviour reported for the CA-I bloodstream forms compared with those of RA.