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Factors Underlying The Contribution Of Psychological Stress To The Induction of Exercise-Related Menstrual Disturbances: 3099 Board #4 June 2 2

PURPOSE: Our previous findings have shown a significant relationship between the severity of exercise-related menstrual disturbance and an increase in perceived stress. In this analysis, we examined underlying factors that contribute to changes in psychological stress with the induction of exercise-related menstrual disturbances in a 3-month exercise and caloric restriction intervention in sedentary, regularly menstruating women (n=36). METHODS: Women (age 18-24 yrs, BMI 18-28 kg/m 2 ) were randomized to either an exercise only group or one of four groups designed to induce an energy deficit through varying combinations of caloric restriction and exercise over 3 menstrual cycles preceded by a Baseline cycle. The intervention included exercise (5 d/wk, 50-85% VO2max, 20-75 min) and controlled diet. Menstrual function and reproductive hormones were characterized using daily urinary estrone-1-glucuronide (E1G), pregnanediol glucuronide (PdG), luteinizing hormone, and menstrual calendars. Depressive symptoms were assessed with the Beck Depression Inventory (BDI). Presence of eating disorders was assessed using the Eating Disorder Inventory (EDI). Psychological stress was determined with the Perceived Stress Scale. Other factors investigated were anthropometrics, fitness, age, and reproductive factors. RESULTS: The intervention caused moderate weight loss (-2.59 ± 0.35 kg), increases in fitness, declines in body fat and declines in E1G and PdG (p< 0.006). Perceived stress increased significantly across the intervention (p<0.001). Changes in perceived stress were associated with baseline luteal phase PdG AUC (r = 0.387, p = 0.024), change in body weight (p = 0.017, R = 0.401), change in fat free mass (p = .020, r = .391), and the change in BMI (r = 0.419, p = 0.012) such that higher baseline PdG concentrations and greater reductions in BMI and body weight were associated with lower increases in perceived stress. Baseline scores of BDI and EDI were not significantly related. Age, fitness, body composition, luteal phase length, and follicular phase E1G were also not significantly predictive of PSS. CONCLUSION: Stress sensitivity as defined by changes in perceived stress and related menstrual disorders may depend on baseline ovarian status and changes intervention induced changes in anthropometrics.