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THE RELEASE AND MOLECULAR STATE OF MAMMALIAN BRAIN ACETYLCHOLINESTERASE

Abstract— By incubating the particulate fraction of caudate nucleus from calf brain in ion‐free media, about 90 per cent of the AChE activity was brought into solution. The effects of different salts, EDTA and tetracaine on the release were studied. The mol. wt. of the enzyme was determined by gel filtration. About 90 per cent of the activity in a fresh preparation appeared in a form with mol. wt. 80,000. During storage this form was gradually transformed into forms with higher mol. wts. The effects of changes in the ionic environment on the aggregation were investigated. Purification attempts always resulted in the transformation of the enzyme into high mol. wt. forms. If the release was performed in the presence of DEAE‐Sephadex‐A25, the enzyme no longer aggregated. The cytosol fraction always contained some AChE activity; the significance of the presence of AChE in this fraction is discussed.