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Regulated Expression of Chromobox Homolog 5 Revealed in Tumors of ApcMin/+ ROSA11 Gene Trap Mice

The gene-trap lacZ reporter insertion, ROSA11, in the Cbx5 mouse gene illuminates the regulatory complexity of this locus in ApcMin/+ mice. The insertion site of the β-Geo gene-trap element lies in the 24-kb intron proximal to the coding region of Cbx5. Transcript analysis indicates that two promoters for Cbx5 flank this insertion site. Heterozygotes for the insertion express lacZ widely in fetal tissues but show limited expression in adult tissues. In the intestine, strong expression is limited to proliferative zones of crypts and tumors. Homozygotes for ROSA11, found at a lower than Mendelian frequency, express reduced levels of the coding region transcript in normal tissues, using a downstream promoter. Analysis via real-time polymerase chain reaction indicates that the upstream promoter is the dominant promoter in normal epithelium and tumors. Bioinformatic analysis of the Cbx5 locus indicates that WNT and its target transcription factor MYC can establish a feedback loop that may play a role in regulating the self-renewal of the normal intestinal epithelium and its tumors.

A core strategy of current efforts to understand development and neoplasia is to document differential patterns of gene expression and deduce the genetic control over those patterns. Documentation of the pattern of gene expression takes several forms: transcriptome analyses of whole tissues reveal changes in transcript levels measured by hybridization to arrays or by the comparison of transcript copy numbers identified by deep sequencing in differentiating or neoplastic lineages. Alternatively, spatial and temporal patterns of gene expression can be visualized at cellular resolution by tagging each active gene using random transposon insertions. Here, a marker tag enables both the cloning of the active gene and visualization of its activity (Starr et al. 2011).
The genetic control of developmental and neoplastic processes is deduced by analysis of changes in spatial or temporal expression patterns that are elicited by mutations in relevant genes. These mutations may be studied singly or in combination. Loss-of-function alleles tagged with reporters provide a particularly powerful entry into the analysis of a system of genetic control. The heterozygote displays the pattern of expression of the gene of interest (Austin et al. 2004), and the homozygote reveals whether the gene's activity is a necessary element in a biological feedback loop with positive or negative parity (Brenner et al. 1990;Thomas 1998).
Soriano et al. initiated the transposon-tagging approach to study development in the mouse by transforming embryonic stem (ES) cells with the promoter-trap vector ROSAb-geo1-29. This reporter gene encodes a fusion protein, b-Geo, with both b-galactosidase (b-GAL) and neomycin phosphotransferase activity (Friedrich and Soriano 1991). One of the strains developed in this program, ROSA11 (R11), has drawn our attention because it expresses a b-GAL differentiation marker that is strongly expressed in the proliferative zone of intestinal crypts and in intestinal adenomas of Min mice (Gould and Dove 1996). By contrast, another of Soriano et al.'s promoter trap mouse strains, ROSA26, is expressed pervasively in the adult mouse and serves as a clonal lineage marker (Merritt et al. 1997;Zambrowicz et al. 1997;Thliveris et al. 2005).
This report provides evidence that the R11 insertion lies within the heterochromatin protein 1a locus Cbx5 on mouse chromosome 15. A detailed informatic analysis of the structure of this locus and a molecular analysis of its expression in normal and neoplastic tissues has uncovered a complex system of regulation of this locus. Our understanding of the biology of the normal self-renewing intestinal epithelium and its neoplastic derivative is enhanced by these observations.

MATERIALS AND METHODS
Mouse strains, breeding, and maintenance The congenic C57BL/6 (B6) R11 strain was derived from a single heterozygous male generously provided by P. Soriano (Baylor University, Houston TX) by backcrossing to B6 for 10 generations. The congenic B6 Apc Min/+ mice were obtained from our colony (Moser et al. 1990). The doubly heterozygous R11/+ Min/+ animals were obtained by crossing R11/+ females to Min/+ males. Homozygous R11/R11 Min/+ mice were obtained by crossing R11/+ females to R11/+ Min/+ males. Mice were maintained under a protocol approved by the Animal Care and Use Committee of the University of Wisconsin School of Medicine and Public Health and in a facility in the McArdle Laboratory approved by the American Association of Laboratory Animal Care. Animals were housed in standard caging with free access to mouse chow and acidified water.
Histochemical staining for b-GAL activity To understand the expression pattern of the R11 promoter trap reporter, b-GAL activity was assayed in adult tissues. Mice were killed by CO 2 , and tissues were rapidly harvested, pinned on paraffin blocks, and fixed in freshly prepared 4% paraformaldehyde in phosphatebuffered saline (pH 7.3) on ice for 1 hr. Fixed samples were washed three times (30 min each) in Rinse Buffer [100 mM sodium phosphate (pH 7.027.5), 2 mM MgCl 2 , 0.01% sodium deoxycholate, and 0.02% Triton X-100]. Tissues were then stained for 12 to 14 hr in a humidified chamber at 37°in staining solution [Rinse Buffer plus 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide, and 1 mg/mL X-GAL (Invitrogen, Carlsbad, CA) from a 25 mg/mL stock in dimethylformamide]. After staining, samples were rinsed in Rinse Buffer, post-fixed overnight in 10% formalin, and transferred to 70% ethanol. Tissues were embedded in paraffin and sectioned serially at 5 mm. Sections were counterstained with Nuclear Fast Red.
Cloning of the R11 insertion site using inverse PCR Inverse polymerase chain reaction (PCR) was used to clone the genomic insertion site of the R11 promoter trap vector. The inverse PCR protocol was modified from that of Joslyn et al. (1991) as follows: total genomic DNA was isolated from spleens of B6 R11/+ mice. A total of 16 mg of DNA was digested at 37°nearly to completion, first with EcoRI and then with HindIII. The restriction enzymes were then inactivated at 65°for 20 min. Digested DNA molecules were ligated (T4 DNA ligase #10799009001, Roche, at a concentration of 5210 ng/mL) to maximize circularization (Ochman et al. 1990). Ligated DNAs were precipitated, washed, and resuspended in 40 mL of TE-4 [10 mM Tris (pH 7.5), 0.1 mM EDTA]. Five microliters of the ligated material was used for PCR experiments. Long-range PCR was performed by the Roche Diagnostics protocol (kit no. 11681834001) by using primers bGeo-D and SupF-A for the HindIII digest. (All primer sequences are provided in Table 1.) The PCR products were resolved on a 1% agarose gel and stained with ethidium bromide. A~1700-bp band was visualized on an ultraviolet light box and excised. Agarose was removed from the gel fragment using kit no. 28704 from QIAGEN (Valencia, CA). The 1700-bp DNA fragment was then sequenced using a standard Sanger protocol (Applied Biosystems, Carlsbad, CA) with separate bGeo-D and SupF-A sequencing primers.
Genotyping the R11 and wild-type alleles in crosses After the R11 insertion site was determined, primer pairs (R11-G2L/ R11-G4 and SupF-A/R11-G4) were synthesized to distinguish between the wild-type and the R11 alleles, respectively. Progeny mice from crosses were genotyped from tail snip DNA using a three primer system with Primers R11-G2L/R11-G4/and SupF-A (Table1). Standard PCR conditions were used with 2.0 mM MgCl 2 and 0.8 mM of each primer (final concentration). PCR was performed with 30 cycles under the following cycling conditions: 94°(30 sec), 60°(30 sec), and 72°(1 min). The PCR product was run on a 2% agarose gel for size fractionation. The product size of the R11 fragment is~500 bp, and the wild-type fragment is~100 bp.
Cloning by 59 RACE-PCR of sequences downstream of the R11 insertion RACE-PCR was used to identify the promoter responsible for gene expression driven by the R11 promoter trap insertion. Total RNA was isolated from the spleens of R11/+ mice using TRIzol reagent (Invitrogen). A total of 80 mg of total RNA was used in the 59 RACE protocol (Marathon cDNA Amplication Kit PT1115-1; Clontech, Mountain View, CA). Here, first-strand synthesis was performed using 1 mL of total RNA (7 mg/mL) added to 1 mL of GSP-1 primer and 10 mL of reagent-grade dH 2 O. This mixture was heated to 80°for 5 min and rapidly chilled on ice. A total of 8 mL of Reverse Transcription (RT) Mix [4 mL of 5X-RT Buffer (Invitrogen), 1 mL of 15 mM dNTP, 2 mL of 0.1 mM DDT, and 1 mL of Superscript II (Invitrogen)] were added and incubated at 42°for 90 min, 15 min at 50°, and 15 min at 70°. RNA was then digested by adding 1 mL of RNase H and incubating at 37°for 20 min. To tail the cDNA with dT, the entire cDNA mixture was added to a Centricon-50 column (Millipore, Billerica, MA), and the filtrate was concentrated in vacuo to a volume of 10 mL. Then, 10 mL of Tailing Mixture [4 mL of 5X Tailing Buffer (Invitrogen), 4 mL of 1 mM dATP, 1 mL of dH 2 O, and 1 mL of 10220 U of TdT (Invitrogen)] were added to 10 mL of cDNA product and incubated at 37°for 10 min. Terminal transferase was then inactivated at 70°for 15 min. 59 RACE first round amplification: A total of 1 mL each of a series of three test concentrations of dA tailed cDNA (direct, 1/25, 1/50) was added to 49 mL of First Round PCR Mixture [5 mL of 10X Gitschier's Buffer (670 mM Tris, pH 8.8, 166 mM ammonium sulfate, 67 mM MgCL 2 ), 5 mL of dNTP, 5 mL of dimethyl sulfoxide, 1 mL of 2mM Q T primer, 1 mL of 25mM Q 0 primer, and 1 mL of 25 mM GSP-2 primer, 31 mL of dH 2 O]. The reaction was cycled as follows: 98°(5 min), hold at 85°to add 1 mL of Taq (AmpliTaq; Perkin Elmer, Waltham, MA), then 29 cycles of 48°(2 min), 72°(40 min), 93°(1 min), 56°(1 min), and 72°(3 min), followed by 93°(1 min) and 72°(5 min) for product extension.
59 RACE second round amplification: A total of 1 mL of a 1/20 dilution of the first round product was added to 49 mL of Second Round PCR Mixture (5 mL of 10X Gitschier's buffer, 5 mL of dNTP, 5 mL of dimethyl sulfoxide, 1 mL of 25 mM Q 1 primer, 1 mL of 25 mM GSP-3 primer, 1 mL of 1.25 U AmpliTaq, and 32 mL of dH 2 O). The reaction was cycled 39 times as follows: 98°(3 min), 93°(1 min), 56°( 1 min), and 72°(3 min). The product was brought to 93°for 1 min followed by extension at 72°for 5 min. Then, 10 mL of this second PCR product was resolved on a 1% agarose gel, revealing a singlẽ 400-bp band. This band was excised, purified, cloned with the TOPO-TA cloning kit (Invitrogen), and Sanger sequenced (using sequencing primer GSP-3) as described previously.
Western blotting of CBX5 protein in R11/R11 homozygotes Western blot analysis as described by Schlamp and Nickells (Schlamp and Nickells 1996) was performed with the following modifications. Protein was isolated from B6 R11/R11 spleens solubilized and sonicated in SBA [10% sodium dodecyl sulfate, 10 mM B-mercaptoethanol, 20% glycerol, 200 mM Tris (pH 6.8)]. Bradford analysis was used to determine the protein concentration in each sample. Each lane was loaded with 50 mg of soluble protein and the protein profile resolved on a 10% sodium dodecyl sulfate polyacrylamide gel, along with standard molecular weight markers. Gels were transblotted onto Immobilon P membranes (Millipore), visualized using Ponceau S, and blocked for 2 hr at room temperature in Tris-buffered saline (TBS) containing 5% skim milk powder. Blots were then washed extensively in TTBS (TBS plus 0.05% Tween 20) and incubated with primary antibody [10 mL of 1/200 dilution of mouse monoclonal to CBX5 protein (Ayyanathan et al. 2003) in 2 mL of Blocking Buffer (TBS and 5% skim milk)] overnight at 4°with continuous slow agitation. The blot was washed as above and challenged for 3 hr at room temperature with agitation using 1/2000 goat antirabbit secondary antibody conjugated to alkaline phosphatase (Jackson Immuno-Research Laboratories Inc., West Grove, PA.) Polypeptides were visualized after color staining by 5-bromo-4-chloro-3-indolyl-phosphate and nitro-blue tetrazolium.

RNA isolation and real-time PCR analysis
RNA was isolated from tissues frozen in liquid nitrogen using Trizol reagent and cleaned up using RNeasy Columns (QIAGEN) per the manufacturer's protocol. RNA quantity and purity were assessed by spectrophotometric analysis. Up to 10 mg of RNA was treated using TURBO DNA-free (Ambion, Austin, TX) and inactivated per the manufacturer's protocol. cDNA was synthesized from 1 mg of DNase-treated RNA using random hexamers and M-MLV reverse transcriptase in the presence of SuperAseIn (Ambion). Quantitative real-time PCR was conducted using an MJ Research Opticon 2 realtime PCR cycler and SYBR Premix Ex Taq (Takara Bio Inc., Shiga, Japan). The reactions contained 200 ng of cDNA and 200 nM final concentration of the primer pairs listed in Table 1. Several housekeeping control genes were tested, including GAPDH, H2A.Z, TBP, PGK-1, mSdha, Alas-1, and b-actin. b-actin was found to be consistent between tumor and normal intestine and was used to normalize all Cbx5 primer sets for each RT-PCR run. The b-actin primers used are shown in Table 1. In addition, b-actin plasmid clones of each of the Cbx5 primer sets were serially diluted and coordinately run with each primer set to determine the absolute copy number for each RT-PCR run. After correction for primer efficiency, the final copy numbers were determined from Ct values normalized to the b-actin reactions for each sample.

RESULTS
Analysis of the R11 genomic insertion site and fusion transcript identifies the Cbx5 locus encoding the CBX5 (heterochromatin 1a) protein Inverse PCR was used to identify the insertion site of the R11 trap vector. DNA was isolated from the spleens of R11/+ mice and digested with HindIII. A 1700-bp genomic fragment was isolated n Table 1 Sequences of primers used for genomic PCR, RT-PCR, and real-time PCR analyses Each letter in parentheses corresponds to the location of that primer relative to the genomic map of the R11-Cbx5 locus in Figure 1. Bolded nucleotides represent the genomic insertion site of the R11 promoter trap vector. PCR, polymerase chain reaction, RT, reverse transcription. and sequenced: a novel joint created by the insertion site of the R11 promoter trap vector was identified (Table 1). This site was located between the two upstream untranslated Cbx5 exons at position 103,049,787 bp (mouse genome assembly NCBI37/mm9; Figure 1A) on chromosome 15 Fujita et al. 2011). Mapping crosses (summarized below) confirmed the position of the R11 insertion to the region containing the Cbx5 gene on chromosome 15. Using spleen RNA from a R11/+ mouse, a 380-bp fusion transcript was isolated by 59-RACE (Frohman et al. 1988). This fragment was sequenced, and a 90-bp unique region was identified. A database search (http://blast.ncbi.nlm.nih.gov/) localized this sequence to the 59 UTR of Cbx5. Real-time PCR of the wild-type allele connected this 59 unique sequence to the first coding exon of Cbx5.
Two promoters control the transcription of the Cbx5 locus To investigate the impact of the R11 promoter trap insertion allele on the CBX5 transcript profile, exon connection experiments were developed, using RT PCR. Primers connecting the Cbx5 coding exons 4 to 5 confirmed that RNA isolated from R11/R11 homozygous, R11/+ heterozygous, and +/+ wild-type mice each contained the full coding sequence of Cbx5 in exons 4 and 5. Interestingly, the RT PCR splice form connecting exon 1 (located 59 to the R11 insertion site) to exon 4 of the Cbx5 coding sequence was absent in spleen RNA from the R11 homozygote ( Figure 1B). This result was confirmed by quantitative RT PCR assays using the same primers in exons 1 and 4 ( Figure 1D).
The reduced level of the exon 124 splice form can be explained by the strong transcription terminator present at the 39 end of the promotertrap sequence (Friedrich and Soriano 1991).
To investigate further the effect of the R11 promoter trap insertion on CBX5 RNA expression, exon connection analyses were used to analyze products from the Cbx5 coding region. Several distinct populations of exon connection products were detected. Exon 1 transcripts were spliced directly to exon 4 but were never associated with exon 2 sequence ( Figure 1B). Exon 2, located downstream of the R11 insertion site, was connected to the downstream Cbx5 coding sequence in exon 4 through splicing with exon 3 and rarely with an alternative exon between 2 and 3 designated 2a ( Figure 1B and data not shown). Taken together, two populations of CBX5 transcripts were confirmed; one initiated at upstream promoter (P1) that contains the exon 425 coding region except when it is efficiently prematurely terminated by the R11 insertion, and one initiated at a downstream promoter (P2) that also contains the full coding region. These findings are consistent with the validated RefSeq sequences of three distinct full-length Cbx5 cDNAs from two distinct promoters (P1: RefSeq NM_001076789; P2: RefSeqs NM_007626.3, NM_001110216.1). Together, these observations support a two-promoter regulatory structure for the Cbx5 locus-upstream P1 and downstream P2.
Primers connecting exon 4 to exon 5 confirmed that RNA isolated from homozygous, heterozygous, and wild-type mice always carried the full coding sequence of Cbx5, although at reduced levels in the homozygote ( Figure 1D). Analysis of CBX5 protein from the spleen  Table 1. 59 untranslated exons (Ex123) and coding exons (Ex427) are designated. (B) Exon connection analysis using primers Hp1-BF and Hp1-4R in exons 124 and primers Hp1-2F and Hp1-BF in exons 224 using cDNA from spleens of +/+, R11/+, and R11/R11 B6 animals. Products were resolved by agarose gel electrophoresis and stained with ethidium bromide. The splice form exon 124 was absent in the R11/R11 homozygote. (C) Western blot analysis of total protein extracts from spleens, probed with a mouse monoclonal CBX5 antibody, as described in Materials and Methods. Intact CBX5 protein was present in both the wild-type and R11/R11 homozygote. (D) Transcript levels in spleen RNA determined by real-time PCR, as described in Materials and Methods. A marked reduction in copy number of coding transcript (Cbx5 exon 425) is noted in the R11/R11 homozygote. This finding is consistent with the notion that the upstream promoter (P1) is responsible for the majority of the coding transcript of Cbx5. revealed that full-sized protein was expressed in the homozygote (Figure 1C). Thus, homozygosity for the R11 insertion does not completely eliminate expression of CBX5 protein in the spleen.
b-GAL staining of adult tissues in R11/+ heterozygous adult mice Widespread b-GAL staining in the R11 heterozygous fetal and neonatal mouse was first described by Friedrich and Soriano (1991). In the current study, E18 heterozygotes exhibited extensive, but not uniform, staining in all tissues examined (Figure 2).
In the adult, the patterns of b-GAL expression were much more restricted, shown by the analysis of paraffin sections of various tissues from adult mice (Figure 3). Strong positive staining was localized to tissues presumed to be dividing, such as the lower proliferative zone in the small intestine and the germinal centers in the spleen. We also found b-GAL activity in nondividing tissues such as brain (Figure 3, A2C), skeletal muscle ( Figure 3E), and the lung ( Figure 3F). These patterns were found in both homozygous and heterozygous mice, whereas wild-type mice were negative.

Effects of the R11 insertion on viability assayed by transmission analysis
The family of Chromobox homolog proteins is involved not only in heterochromatic gene silencing but also in a broad range of functions encoded in euchromatin (reviewed by Kwon and Workman 2011). We therefore investigated whether the R11 insertion element that blunts P1-driven expression from the locus affected the production of viable heterozygous and homozygous offspring. To enable a quantitative analysis of the effect of the R11 insertion on transmission to viable B6 mice, genotyping primers were designed to distinguish among homozygous, heterozygous, and wild type. The transmission to viable progeny was investigated in two types of cross (Table 2). First, heterozygous mice were intercrossed. At 7 to 10 days, homozygous offspring were observed at a frequency of only 5%, rather than the expected 25%. Second, a similar deficiency in viable homozygous progeny was observed when heterozygous mice were backcrossed to surviving homozygotes. Thus, homozygotes survive at reduced frequencies, as scored at 7 to 10 days. Further, among the surviving adult R11/R11 homozygotes, there were no overt phenotypic abnormalities. These survivors do not appear to have acquired linked genetic suppressors of lethality because they continue to transmit the R11 insertion to homozygous progeny at reduced frequency.
Induction of Cbx5 in intestinal adenomas of Apc Min/+ mice Strong b-GAL expression in the intestine of mice carrying the R11 insertion is limited to the lower proliferative zone of crypts. Mice harboring the Min mutation of Apc in addition to the R11 insertion give rise to b-GAL positive intestinal adenomas (Gould and Dove 1996, and Figure 4). Thus, the lacZ reporter element in the R11 insertion serves as a developmental marker for the cells in the lower proliferative zone of the intestine and in Min-induced tumors. The level of CBX5 RNA expression within this developmental-neoplastic lineage was investigated by quantitative transcript analysis of normal and tumor tissue. CBX5 RNA transcripts harvested from equivalent amounts of tumor and normal intestinal tissue of B6 Apc Min/+ mice heterozygous and homozygous for the insertion were assayed by real time PCR ( Figure 5). As a control, several housekeeping genes were assayed. b-actin was found to be the most consistently expressed across all tissues and was used to normalize RNA levels (see Materials and Methods). A two-way analysis of variance revealed a significant increase in average expression of CBX5 RNA in R11/+ heterozygous tumor cells of 1.94 6 0.55 SE (log2 scale) compared with normal cells (P ¼ 0.001). In R11/R11 homozygotes, the average expression of RNA from the exon 425 coding region of Cbx5 was significantly reduced (22.72 6 0.64) as compared to wild type (P ¼ 0.0001). In tumors, a nonsignificant reduction of CBX5 RNA level is observed in heterozygotes compared with wild type (20.77 6 0.5; P ¼ 0.18). All Mininduced tumors differ from all normal tissue samples, and the three different genotypic classes differ from one another. However, within the restricted statistical power created by small numbers of certain classes in Figure 5, it cannot be concluded that these two sets of class differences are correlated.

DISCUSSION
The structure of the R11 insertion has been established through the following series of steps. First, RACE PCR has shown that expression from the b-Geo promoter trap lies under the control of the Cbx5 gene. A cDNA fusion product was identified that joined b-Geo sequence to an untranslated 59-proximal exon of Cbx5 (Figure 1). Next, inverse PCR analysis localized the b-Geo promoter trap vector to a 59-proximal intron of Cbx5 (Figure 1). Finally, exon connection experiments involving the 59 exons of Cbx5 gave products that are consistent with a model in which two distinct promoters (P1, upstream, and P2, downstream) regulate the transcription of this gene ( Figure 1A). This model is consistent with the spliced expressed sequence tags identified in the GenBank genome database and visualized through the UC-Santa Cruz genome browser (http://genome.ucsc.edu/ and Kent 2002; Benson et al. 2004).
The sequence of the Cbx5 locus suggests a number of interactions with transcription factors that reflect the in vivo expression pattern. The mouse Cbx5 upstream promoter, P1, appears to be a bidirectional promoter shared with hnRNPa1 that, by in silico analysis, has identifiable binding sites for Myc and Tcf4 (Matys et al. 2006). Human Chip-Seq ENCODE data from multiple laboratories show direct binding of RNA polymerase 2, Myc, Tcf4, and c-Fos at P1 (Rosenbloom et al. 2010). This is consistent with our observation of expression from the P1 promoter and expression of b-GAL activity in WNT-responsive Min-induced tumors (Figure 4). In these tumors Tcf and b-catenin are controlled by WNT signaling, leading to the expression of Myc (reviewed by Myant and Sansom 2011). The downstream promoter, P2, shows RNA polymerase 2 binding in human tumor and ES cell lines, supporting the observation that CBX5 function is not completely lost in the R11 homozygous mouse (Figure 1, B and D). We hypothesize that, in the R11/R11 homozygote, independent initiation of transcription at the P2 promoter drives exon 425 transcription during development that is suboptimal for viability (Table 2).
It is interesting to speculate on the role of the Cbx5 protein (heterochromatin 1a) in the biology of the crypt progenitor that is amplified in Min-induced tumors. This protein has been reported to interact with the origin recognition complex, ORF (Pak et al. 1997). n Table 2 Transmission of the R11 allele from R11/+ X R11/+ intercrosses and R11/+ X R11/R11 backcrosses on the B6 background Cross R11/+ X R11/+ R11/+ X R11/R11 Reported numbers represent live births tested at 7210 days of age. The distribution of observed genotypes among the progeny from each cross differed significantly from the expected ratios for Mendelian segregation (P , 0.001, x 2 test). NA, not applicable.
High-resolution mapping analysis has shown that Cbx5 protein is also associated with transcriptionally active Drosophila chromatin (Cryderman et al. 2005). Cbx5 has been shown to be up-regulated in the breast cancer cell line MCF7 with corresponding increase in protein levels (Thomsen et al. 2011). The requirement for controlled replication of stem cells may involve an interaction between these classes of positive action by Cbx5 protein and an antagonist such as POF in Drosophila (Johansson et al. 2007).
Beyond the elements presumed to direct transcription, the Cbx5 locus contains other conserved elements not likely to involve canonical promoters. Using the Multiz 30-way Alignment track in the UCSC genome browser (Blanchette et al. 2004, mouse genome assembly NCBI37/mm9, July 2007), five highly conserved noncoding regions of unknown function are identified in the first intron (24,380 kbp in total length). These conserved regions range between 500 and 1000 bp in length and are shared among mammals; four of the regions are found to be conserved further-through marsupials. The fifth region (between 103,057,150 and 103,057,450 on Chr. 15) shows a high level of conservation among mammals and extends to the chicken. No significant open reading frames can be found spanning these conserved regions. Using exon connection, we have observed no evidence for alternative splicing between exons 1 and 4 or for alternatively initiated transcripts upstream of P2. In addition, there is no evidence for a transcribed miRNA in the region on the basis of published miRNA database and prediction analysis (Griffiths-Jones 2004, 2006Weber 2005;Griffiths-Jones et al. 2008). Despite the lack of evidence for transcription of these regions, their high level of conservation suggests that these are important noncoding regulatory elements. We shall return to discuss possible regulatory processes involving these conserved sequences in intron 1.
Finally, the Cbx5 region of the mouse genome is highly susceptible to transposon integration, deserving comment ( Figure 6). The R11 insertion lies near the second exon of Cbx5, within the conserved upstream promoter region. This region is an active area for transposon integrations. From among approximately 51,000 localized insertions that have been verified in the mouse genome, the International Gene Trap Consortium has identified and mapped 656 independent insertions within the Cbx5 locus ).This number represents a frequency of more than 1 per 1000 of the total reported insertions, nearly 20 times the frequency of insertions expected if the insertions into genes were random. Plausibly, this preference reflects the transcriptional activity of the Cbx5 locus during development: active open chromatin is a preferred target for integration (Sandmeyer et al. 1990). Of the five highly conserved regions within intron 1 of the locus (Figure 6), insertions of transposons are found in or near the four conserved noncoding regions but not in the most highly conserved fifth region. Perhaps even in ES cells loss of a regulatory role of this element is lethal, or this element is refractory to integration because of its structure at the time of integration.
The function of the Cbx5 locus has also been reflected by a histochemical analysis of b-GAL activity in fetal and adult tissues (Friedrich and Soriano 1991, and see Materials and Methods). In the E18 fetus, the b-Geo gene is expressed ubiquitously in the skin. The denuded E18 fetus reveals localized expression to appendages and rib cage. By contrast, ubiquitous expression of the b-GAL marker gene is found in the fetal spleen and kidney (Figure 2). CBX5 function is particularly important for accurate mitotic division, as judged by siRNA down-regulation of the alpha, but not the beta and gamma isoforms in HeLa cells (De Koning et al. 2009). Current studies of chromatin structure associate CBX5 protein with late-replicating heterochromatin in mouse embryonic fibroblasts (Bachman et al. 2001). When somatic nuclei are reprogrammed with extracts from Xenopus oocytes, the level of CBX5 (heterochromatin 1a) protein in these nuclei decreases (Bian et al. 2009). Thus, we expect that the Cbx5 gene has an essential function for viability and that this function is served by expression from both P1 and P2. Although the expression of P1 serves primarily in fetal tissues and dividing adult cells, it can also be observed in localized areas of even  nonproliferating adult tissues tested including brain, cardiac muscle, and lung interstitium (Figure 3).

Function in development and the two-promoter hypothesis
The R11/R11 homozygote is not lethal but is found at frequencies reduced from those expected for Mendelian transmission: in heterozygous intercrosses, 5% of R11/R11 mice survive to weaning instead of the predicted 25%. Among backcrosses of heterozygotes to homozygotes, there were 17% R11/R11 mice instead of 50% (Table 2). The surviving homozygotes seem to be normal and fertile, and their progeny continue to display a low incidence of homozygous pups. Fulllength CXB5 protein is expressed in the spleens of these R11/R11 mice, as judged by Western analysis. However the predominant splice form for the Cbx5 coding region, driven by the proposed upstream promoter, is absent in the R11/R11 homozygote (Figure 1, B and D). In the spleen, the level of exon 425 transcript from the coding region of Cbx5 arises from a proposed promoter downstream of the R11 insertion ( Figure 1B) and is reduced by an order of magnitude in the R11/R11 homozygote ( Figure 1D; Figure 5).

Function in neoplasms
In Min-induced adenomas of the small intestine, exon 425 transcripts of the Cbx5 coding region are elevated compared to normal intestine. We interpret this elevation as a reflection of the amplification of the progenitor cell type in the tumor. As expected for this amplification model, this level of expression is observed in adenoma tissue for all three genotypes: +/+, R11/+, and R11/R11. The evidence for further deregulation of exon 425 transcript levels in R11/R11 tumors compared to normal epithelium is not statistically significant ( Figure 5; The expression of transcripts containing exon 425 in Mininduced adenomas deserves comment in light of the binding specificities inferred from the ENCODE Chip-Seq data (Rosenbloom et al. Figure 6 Physical map of mouse Chromosome 15 viewed centromere to telomere containing the Cbx5 locus as visualized using the UCSC mouse genome browser Fujita et al. 2011). The orientation of transcription is telomere to centromere with P1 oriented in the region 103,070,863 to 103,070,247, lying between Hnrnpa1 and the first exon of Cbx5. P2 is oriented at 103,046,330 to 103,045,783. The tracks included are: basepair position, chromosome band, RefSeq genes (Pruitt et al. 2005) in blue, placental mammal basewise conservation by PhyloP (Siepel et al. 2005), Multiz alignments of human, rat, opossum, chicken, and stickleback (Blanchette et al. 2004), and the International Gene Trap Consortium sequence tag alignments (Skarnes et al. 2004), where we added an asterisk to indicate the position of the R11 insertion. Peaks of mammalian conservation are recognized by PhyloP with high peak heights in the coding region of Cbx5 and five peaks within intron 1. Two of these five intronic peaks show conservation between mammals and chicken, as shown by the Multiz alignment track. Gene trap sequence tags are color coded by the institution or source of the gene trap insertion and are available through the International Gene Trap Consortium (http://www. genetrap.org/). 2010). For the proposed upstream promoter, P1, both TCF and MYC binding are inferred and have been shown to be active in breast cancer cell lines (Thomsen et al. 2011). Because MYC is a direct downstream target of WNT-dependent TCF function (via b-catenin), this pair of binding activities formally constitutes a feedback loop. If MYC induces transcription from P1, the inferred feedback loop would have positive parity and contribute to the developmental choice in the normal crypt progenitor population and its amplified neoplastic derivative. By contrast, if MYC represses transcription from P1, the inferred feedback loop would have negative parity and would serve a homeostatic function in regulating WNT-induced CBX5 function. (The WNT-inhibitory function WIF1 may serve that same homeostatic regulatory logic.) The observation that exon 425 transcript levels are also expressed in Min-induced tumors homozygous for the R11 insertion is paradoxical in light of the aforementioned ENCODE analysis (Rosenbloom et al. 2010). How would these levels be driven if the distal P2 region shows no TCF or MYC binding specificity? One possible resolution to this issue would involve an additional transcription factor controlled by b-catenin, driving transcription from P2. Another conceivable resolution to this issue would involve long-range chromatin interaction with P2, either by P1 or by a locus control region elsewhere in the genome that is responsive to WNT and b-catenin.
The intricacies of gene expression uncovered by this initial analysis of the R11 insertion in the Cbx5 locus of the mouse genome have been developed by a molecular analysis of the spleen, the intestine, and Min-induced intestinal tumors, coupled with a bioinformatic analysis of the ENCODE database for the human genome. The observed expression of the locus in Min-induced adenomas is consistent with the bioinformatic prediction of WNT-induced transcription from the strong upstream promoter P1 and the known action of APC protein to regulate WNT signaling. Novel insight has come from the bioinformatic identification of a role for the WNT-dependent factor MYC also acting at promoter P1. The inferred feedback loop needs to be understood more fully in regard to its role in the homeostasis of the self-renewing normal intestinal epithelium and any subtle loss of control in the adenoma. Beyond these issues involving the strong promoter P1, the secondary promoter P2 raises the further issues of what drives its expression in normal tissues and in the adenoma ( Figure 5). Vermeulen et al. (2010) have observed that only a minority of cells in human colon cancers have activated WNT; these cells show stem cell activity in xenograft assays. We have argued that this class of cell is amplified in Min-induced adenomas. The promoters for the terminal developmental genes for fatty acid binding protein and villin, currently used to drive Cre recombinase in generating conditional gene knockouts, do not have this specificity for WNT and MYC. Thus, P1 (and perhaps P2) will be a useful new driver for experimentally manipulating the Min tumor lineage.