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Screening of thermotolerant yeast by low-energy ion implantation for cellulosic ethanol fermentation

ABSTRACT One thermotolerant mutant, ST-1559, was selected after mutagenizing with ion beam implantation. The results showed that ST-1559 could grow well under 45°C,and its ethanol yield reached 109.46 g/L when the glucose content was 250 g/L in the fermentation medium. When corn straw which was pretreated by NaOH was used as substrate in fermentation experiment under 45°C, it produced 13.48 g/L ethanol, and 82.17% of the cellulose was achieved. These results suggest that ST-1559 had advantage in simultaneous saccharification and fermentation.