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Green Synthesis of Piperine Loaded Gold/Triton X-100 Nanoconjugates: In-vitro Evaluation of Biocompatibility and Anti-Oxidant Activity

In this research, gold nanoparticles (Au NPs) were synthesized by a green chemical approach using the organic natural product piperine isolated from the black pepper. The piperine-Au NPs attached on the Triton X-100 and they were characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), dynamic light scattering spectroscopy (DLS), X-ray powder diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The TEM analysis confirmed the spherical shape of Au NPs with a diameter of 17-30 nm. The XRD analysis revealed that the Au NPs exhibited fcc crystal structure with an average crystallite size of 26 nm. The biocompatibility of the piperine-AuNPs-Triton X-100 nanoconjugates were examined on Hep-G2 cells. The piperineAuNPs-Triton X-100 nanoconjugates exhibited better anti-oxidant activity.