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Determination of Organic Acids in White Tea by HPLC Method

This paper aimed to determine how to test 8 organic acids in white tea by using high performance liquid chromatography(HPLC). The best chromatographic conditions are as follows: Agilent-1200 HPLC equipped with UV detector and a ZORBAX SB-C18column(5 μm,4.6 mm×250 mm). The mobile phase was 3% methanol-0.05 mol/L KH2P04(p H value adjusted with phosphoric acid to 2.75) with flow rate of 0.5 m L/min. Column temperature was at 25 ℃, the detection wavelength was at 215 nm and 15 μL sample was injected into the column.The recovery rate of the organic acids could reach 91.63% ~103.34% with relative standard deviations of 0.45% ~2.34%. The organic acids linear correlation coefficient was above 0.9997, indicating that the method was simple,efficient and accurate.