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Immuno-ultrastructural localization of p53 protein in patients with laryngeal carcinoma.

Protein p53 was localized ultrastructurally with 5 nm gold-streptavidin particles in cells of laryngeal carcinoma embedded in Epon 815. Postembedding technique to study p53 protein was used. Protein p53 was found predominantly in nucleus of the cells but the label was also observed in cytoplasm of the cells. Five of 15 samples from patients with laryngeal carcinoma showed positive labeling for p53, (33.3% of all examined samples). Controls of tumor cells incubation with normal mouse serum showed no labeling with gold streptavidin particles.