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Time-of-flight 3D localization of fluorescent inclusions in thick turbid media with ultra-fast TCSPC APDs and electronics

We herein describe a time-of-flight (TOF) technique to localize in 3D the position of a small fluorophore-filled inclusion immersed in a scattering medium. To achieve this, we exploit the arrival time of early excited and fluoresced photons. This is an embodiment of fluorescence diffuse optical tomography (FDOT) which aims to find the position of fluorescent heterogeneities in 3D in thick turbid media non-invasively via optical imaging techniques. In Ref. 4, we gave a short review of previous work on the problem of localizing a fluorescent inclusion via time-resolved measurements. This will not be discussed again here.