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[The correlation between multidrug resistance and glutathione (GSH) in EAC, EAC/ADR and EAC/ADR(R) cell lines].

Determination of GSH was performed by a modified fluorometric method. The experiments showed that the GSH content in resistant cell line (EAC/ADR) was 4 fold higher than that in sensitive cell line (EAC), being 1.25 +/- 0.35 microgram/10(6) cells in EAC/ADR and 0.31 +/- 0.12 microgram/10(6) cells in EAC. When the drug treatment was discontinued for 36 weeks, the sensitivity was partially recovered and the GSH level in EAC/ADR(R) cell parallelly decreased. In addition, vincristine, mitomycin C, actinomycin D and VP-16 showed no effect on the GSH content in the EAC/ADR cell.