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Computer simulation of fluorescence depolarization due to brownian motion.

A computer program has been written to simulate the Brownian motion of rigid fluorescent molecules. The time dependence of the fluorescence polarization anisotropy as generated by this simulation is in agreement with that predicted by the recent theoretical treatment of Belford, Belford, and Weber (Proc. Nat. Acad. Sci. USA (1972) 69, 1392-1393). The program thus serves as a verification of their equation. It is being generalized to cover the case of nonrigid molecules.