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GENETIC VARIATION IN SELECTED SPECIES OF PRAWNS OF GENUS MACROBRACHIUM USING MITOCHONDRIAL 16S rRNA

Mitochondrial partial 16s ribosomal gene was used to infer the genetic variation of species of genus Macrobrachium. From 481 bases, the number of variable sites for Macrobrachium rosenbergii is 236, Macrobrachium villosimanus is 34, Macrobrachium lar is 119 and Macrobrachium idella is 105. The average nucleotide frequencies were A: 35.1%; T: 29.8%; C: 22.4%, G: 12.6% and the A+T rich sequences was 64.9%. The Ts/Tv ratio is 27. More number of haplotypes was seen in Macrobrachium rosenbergii population. Neutrality index Tajima’s D value of Macrobrachium rosenbergii population was negative. High haplotype and low to moderate nucleotide diversity were seen for all the populations of different species and the populations are expanding in size.