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Effects of vasoactive peptide urocortin on hemodynamics in spontaneous hypertensive rat and its relationship with L-type calcium channel

Aim To investigate the effects of urocortin(UCN) on hemodynamics and the remodeling of myocardium,and its mechanism in spontaneous hypertensive rat(SHR).Methods UCN(1 μg·kg-1·d-1,3 μg·kg-1·d-1,6 μg·kg-1·d-1) was intravenously injected daily for 2 weeks and its effects on hemodynamics in spontaneous hypertensive rat were observed.The whole-cell patch clamp technique was used to compare the effects of UCN on left myocardial cell's L-type calcium channel.The flow cytometry method was used to test the content of fluorescence calcium in myocardium.Results UCN improved the systolic and diastolic function of SHR,obviously decreased the AMP,LVSP,LVEDP(P0.01),and increased +dp/dtmax and-dp/dtmax(P0.01),improved the hymodynamics for hypertension in SHR.UCN could inhibit the opening of L-type calcium channel and decrease the electric current of cardiamyocytes(P0.01).Further more,UCN could decrease the contents of fluorescence calcium in SHR myocardium(P0.01).However,AST,the corticotropin-regulating receptor's antagonist(CRF2R),could reverse the UCN's inhibitory effects(P0.01).Conclusion UCN plays an role in treating hypertension via CRF2 receptor and inhibiting the myocardial calcium channel.