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PC 10 monoclonal antibody to proliferating cell nuclear antigen as probe for cycling cell detection in developing tissues A combined immunocytochemical and flow cytometric study

Proliferating cell nuclear antigen (PCNA), also referred to as cyclin, is an auxiliary protein to DNApolymerase delta and a proposed marker of replicating cells. We have investigated the applicability and limitations of PC10 monoclonal ant ibody to P C N A in a cell kinetics study of developing human and rat tissues by immunocytochemical and flow cytometric techniques. Our data demonstrate that the epitope recognized by PC10 ant ibody is resistant to wax embedding, but sensitive to aldehyde fixation; conversely, alcoholic fixative solutions preserve the immunoreactivi ty to PC10. Tissue distribution, D N A content and bromodeoxyuridine uptake confirm that PC10-immunoreactive cells in alcoholfixed tissues are cycling (G1-, Sand G2-phases traversing) cells. It is concluded that the PC10 ant ibody can be regarded as a powerful tool to study cell kinetics and differentiation in developing tissues, provided that the tissue processing is adeguate.