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Molecular detection of UreC and UreR genes of Proteus mirabilis in diabetic type 2 and non-diabetic patients with urinary tract infection in Baghdad city, Iraq

The aim of this study is the Proteus mirabilis genes ureR and ureC and their correlation urease enzyme production Diabetes type-2 from Urinary Treact Infection (UTI). Out of 1720 samples from adult (>18years) males and females diabetic type II and non-diabetic with UTIs only 39 samples of Proteus mirabilis isolated from urine were obtained according to conventional methods (depending on culturing and microscopic examination) and confirmed by VITECK 2 system that collected from various hospitals and privet labs in Baghdad city, Iraq in the period between December 2020 to April 2021. The results indicate to the incidence of UTI is higher among females 1066 (61.98%)than males 654 (38.02%) and in type 2 diabetic 1016 (59.07%) more than non diabetic 704 (40.93%) and the incidence of P. mirabilis infection is higher among females 25 (64.10%) than males 14 (35.90%) and in type 2 diabetic 23 (58.97%) more than non diabetic 16 (41.03%). All the 39 isolate of P. mirabilis had the ability to produce urease enzyme. Moreover, the results of the ureC and ureR polymerase chain reaction technique showed that all (39) isolates (100%) possessed both ureC and ureR through the appearance of amplicons with a molecular weight of 517 and 359 bp, respectively.