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Transcriptomic landscape of GC‐2spd(ts) cell in response to the downregulation of piR‐1207/2107

Spermatogenesis is a highly orchestrated dynamic developmental process, during which piRNAs play an indispensable role. Our previous studies have confirmed that the levels of piR‐1207 and piR‐2107 were significantly decreased in spermatozoa and seminal plasma of patients with asthenozoospermia, compared with the fertile controls. In order to explore the function of piR‐1207 and piR‐2107 in human spermatogenesis and their potential regulatory downstream genes, we examined the transcriptomic landscape in mouse spermatocyte cell line GC‐2spd(ts) transfected with anti‐piR‐1207 and anti‐piR‐2107 by RNA sequencing. The result showed that 86 and 75 differential expression genes (DEGs) in anti‐piR‐1207 and anti‐piR‐2107 group, respectively. Among the DEGs, three genes including Pbp2, Pde3a and Cage1 were identified as potential key genes playing important roles in mediating sperm motility and morphology in anti‐piR‐1207 or anti‐piR‐2107 transfected transcriptomic response. Next, the expression levels of Pbp2, Pde3a and Cage1 were confirmed by qRT‐PCR. These results showed that Pbp2, Pde3a and Cage1 may serve as the potential regulatory genes of piR‐1207 or piR‐2107. In summary, piR‐1207 and piR‐2107 might have an implication in modulating the process of spermatogenesis through regulating the expression of potential genes.