{'fraction_non_alphanumeric': 0.03271546700704812, 'fraction_numerical': 0.02558410566624119, 'mean_word_length': 4.840058337384541, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 2, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 3, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence

Abstract The antimicrobial properties of silver have been exploited for many centuries and continue to gain interest in the fight against antimicrobial drug resistance. The broad-spectrum activity and low toxicity of silver have led to its incorporation into a wide range of novel antimicrobial agents, including N-heterocyclic carbene (NHC) complexes. The antimicrobial activity and in vivo efficacy of the NHC silver(I) acetate complex SBC3, derived from 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*), have previously been demonstrated, although the mode(s) of action of SBC3 remains to be fully elucidated. Label-free quantitative proteomics was applied to analyse changes in protein abundance in the pathogenic yeast Candida parapsilosis in response to SBC3 treatment. An increased abundance of proteins associated with detoxification and drug efflux were indicative of a cell stress response, whilst significant decreases in proteins required for protein and amino acid biosynthesis offer potential insight into the growth-inhibitory mechanisms of SBC3. Guided by the proteomic findings and the prolific biofilm and adherence capabilities of C. parapsilosis, our studies have shown the potential of SBC3 in reducing adherence to epithelial cells and biofilm formation and hence decrease fungal virulence.


Introduction
Advances in medicine and technology have improved life quality and reduced mortality rates due to microbial infections over the last 100 years. 1 Despite this, the incidence of fungal infections is increasing, particularly those infections due to Candida spp. and Aspergillus spp.. 2 These fungi generally exist as part of the human microbiome, although when left unchecked, they have the capacity to establish infections ranging from superficial mycoses of the skin, hair, and nails to life-threating systemic infections affecting vital organs. 3 , 4 Superficial infections are normally easily managed with a fully functioning immune system but can become recalcitrant in immunocompromised individuals and demand potent antifungal therapy. 5 The increasing rates of fungal infections are largely driven by technological advances such as invasive surgeries and medical equipment, the overuse of antibiotic treatments and by environmental factors such as climate change that provide more favourable conditions to thermotolerant species and/or enhance thermotolerance closer to human body temperature. 6 , 7 The survival of infected patients relies on rapid diagnosis and a suitable treatment regimen, although these factors are compromised by prolonged detection methods ( with low sensitivity ) and the emergence of resistant isolates to first-line treatments. 8 -10 The yeast Candida parapsilosis is an opportunistic fungal pathogen, and although it is considered less virulent than Candida albicans , it has rapidly emerged in recent decades as a serious problem in immunocompromised patients. 11 , 12 Candida parapsilosis is a commensal of the gastrointestinal tract and skin and is frequently isolated from human hands-a common source of contamination of medical devices where biofilm growth can occur. 11 , 13 , 14 Conventional treatment of this pathogen relies on the use of azole, polyene, or echinocandin antifungal agents; however, the spread of antifungal resistance and limited treatment options have diminished their efficacy. 15 , 16 The development of novel, non-toxic antifungal agents with distinct modes of action is urgently required to allow the treatment of resistant isolates.
There has been a reawakening of interest in the antimicrobial properties of silver in recent years. 17 , 18 Silver is incorporated into many topical creams, wound dressings, and as medical device coatings in nosocomial settings, while ongoing research seeks to develop novel silver-based drugs with enhanced efficacy and bioavailability. 19 -21 Silver exhibits several modes of action, including cell wall rupture to induce cell leakage, facilitation of the generation of reactive oxygen species ( ROS ) , and DNA disruption leading to reduced replication. 22 -25 Other mechanisms have been reported, including inhibition of respiration and protein synthesis. 26 -29 The antiviral properties of metallodrugs have also been explored, revealing their roles in disrupting the SARS-CoV-2 papain-like protease ( PL pro ) . Gold and silver N -heterocyclic carbene ( NHC ) complexes have shown promising inhibitory activity in disrupting this enzyme required for viral replication. 30 Conjugation of silver to ligands is an effective strategy to improve drug stability and promote more controlled and targeted release of silver. 31 , 32 It is essential to ensure a suitable ligand with an adequate level of water solubility, lipophilicity, and stability in normal physiological environments. 33 , 34 NHCs constitute a versatile group of organic ligands that readily bind to transition metals. 35 Structural modifications have produced a library of NHCs with distinct properties; however, a divalent carbon atom bound to nitrogen atom ( s ) within a heterocycle forms the basis. 33 , 36 Guided by the work of Wanzlick and Öfele in the 1960s, Arduengo et al. successfully isolated the first stable carbene through the deprotonation of azolium salts in 1991. 37 , 38 The ease and efficiency of this new method triggered a wave of NHC research in the development of novel anticancer and antimicrobial agents. 39 -41 Numerous studies have shown the broad-spectrum antimicrobial activity of NHC silver complexes for the treatment of grampositive bacteria [ Enterococcus faecalis , Staphylococcus aureus , and methicillin-resistant Staphylococcus aureus ( MRSA ) ], gram-negative bacteria ( Escherichia coli and Pseudomonas aeruginosa ) , along with several fungal pathogens ( C. albicans , Candida tropicalis , and C. parapsilosis ) , among others. 42 -45 The NHC 1,3-dibenzyl-4,5-diphenyl-imidazol-2-ylidene silver ( I ) acetate ( SBC3 ) ( Fig. 1 ) , isolated by the Tacke group via Young's synthetic method, has shown excellent inhibitory activity against a selection of resistant bacterial and fungal pathogens, both in vitro and in vivo using the Galleria mellonella insect model. 44 , 46 , 47 SBC3 promoted G. mellonella survival against S. aureus and C. albicans infections, without displaying any adverse effects at the test concentrations, whilst subsequent studies in a murine thigh infection model showed both good tolerability and decreased dissemination of MRSA in response to SBC3 concentrations at the appropriate dosing regimen. 46 , 47 The aim of the work presented here was to apply label-free quantitative mass spectrometry ( LFQ MS ) to C. parapsilosis exposed to SBC3 to characterize the proteomic alterations induced by the complex, determine the anti-virulence activity, and thus gain an insight into its antifungal mode ( s ) of action.

Results and discussion
Antifungal evaluation of SBC3 on C. parapsilosis SBC3 treatment resulted in up to 90% growth inhibition of C. parapsilosis in vitro at the highest test concentration ( Fig. 2 ) . The determined MIC 50 and MIC 80 values, representing 15 μg/ml and 25 μg/ml, respectively, were selected for subsequent proteomic analysis. The level of growth was also assessed under aerobic conditions ( Fig. S1 ) at these concentrations in accordance with proteomic sample preparation.

The proteomic response of C. parapsilosis to SBC3
Candida parapsilosis was exposed to SBC3 ( 15 and 25 μg/ml ) for 8 h and proteins were extracted and purified as described. Purified proteins were identified and quantified via LFQ MS. Statistical analysis confirmed a total of 1281 proteins ( post-filtration of contaminants ) , 451 of which were considered statistically significant and differentially abundant ( SSDA ) with fold changes ≥1.5.
The entire data set is presented as a principal component analysis ( PCA ) ( Fig. 3 ) , whereby replicate samples are reduced into their corresponding sample groups. The positioning of samples depicts the variability relative to one another, and here, the combined variance between components 1 and 2 amounts to 63.6%. Figure  3 demonstrates distinct differences in the proteomes of C. parapsilosis untreated control samples to those challenged with SBC3. The distinction between treated and untreated samples can be visualized via hierarchal clustering of z -score normalized intensity values for 451 SSDA proteins [analysis of variance ( ANOVA ) ; Benjamini-Hochberg procedure, false discovery rate ( FDR ) cut-off of ≤0.05] ( Fig. 4 ) . Two row clusters ( A and B ) were resolved and are based on similar protein abundance trends, with increased and decreased abundance depicted in red and green, respectively. Significant differences in the proteomes of control and SBC3-treated samples are evident. Figure 4 also summarizes the main enrichment gene ontology ( GO ) terms identified within the clusters, which are predominantly linked with protein synthesis, ribosomal components, and carbohydrate metabolism. All cluster proteins identified are listed in Supplementary File 2.
The distribution of all filtered proteins ( 1281 ) is represented on volcano plots ( Fig. 5 ) , where statistically significant ( ANOVA, P < 0.05 ) , and differentially abundant proteins ( fold change ≥1.5 ) are located outside of the vertical and horizontal lines. Proteins that increased and decreased in abundance were similar in both treatment groups but different in the extent of their abundance. Among the top 20 proteins most increased in abundance in 15 and 25 μg/ml SBC3 treated C. parapsilosis , respectively, were chitinase ( + 222-and + 139-fold ) , extracellular membrane proteins linked to iron homeostasis, adherence, and virulence common in fun-gal extracellular membranes ( CFEMs ) 2 ( + 207-and 179-fold ) and CFEM5/6 ( + 163-and 172-fold ) , thioredoxin domain-containing protein ( + 30-and 28-fold ) and uncharacterized protein encoded by candida drug resistance 1 ( CDR1 ) gene for xenobiotic detoxification ( + 25-and 33-fold ) ( Table S1 ) .
Treatment of C. parapsilosis with 15 μg/ml SBC3 decreased the abundance of several proteins associated with amino acid biosynthesis, with the greatest being 3-isopropylmalate dehydratase ( -16-fold ) . Also decreased in abundance were oxidoreductase glutamine amidotransferase type-2 domaincontaining protein ( -13-fold ) , an acyltransferase ( -10-fold ) , and Candida_ALS_N domain-containing protein involved in cell adhesion and pathogenicity ( -9-fold ) . In the cells exposed to 25 μg/ml SBC3, there was decreased abundance of ribosomal protein L37 ( -49-fold ) , proteins associated with respiration and electron transport ( -25-fold ) , uncharacterized protein encoded by CPAR2_407940 associated with cellular stress ( -8-fold ) and squalene monooxygenase used in sterol biosynthesis ( -7-fold ) ( Table  S1 ) . These changes are indicative of a high level of cellular stress in response to SBC3. Increased abundance in cell redox and antioxidant proteins is suggestive of coping mechanisms adopted by C. parapsilosis to its toxic environment, while decreases in amino acid biosynthesis, ribosomal and respiration proteins, among other virulence traits, provide an indication of the mechanistic roles of SBC3 in inhibiting cell growth and reducing virulence.

Protein network interaction in response to SBC3
SSDA proteins identified in Perseus were extracted and analysed further using Cytoscape bioinformatics software to map proteins and their associated pathways increased ( red ) and decreased ( blue ) in abundance in response to SBC3 treatment. Protein clusters associated with glycolysis/carbohydrate metabolism, ergosterol biosynthesis ( also including oxidoreductase activity ) , components linked with amino acid biosynthesis and the TCA cycle were elevated following treatment ( Fig. S2 ) . A considerable number of ribosomal proteins and others associated with protein synthesis ( such as transcription, translation, and elongation ) were decreased in treated samples relative to controls, in addition to respiration and amino acid biosynthesis proteins. In general, the effects of SBC3 on both treatment groups were comparable, with the higher treatment concentration having a more pronounced effect on the differential abundance of proteins.

The effect of SBC3 on cellular respiration
Reduction in respiration in response to SBC3 was ascertained via a 2,3,5-triphenyltetrazolium chloride ( TTC ) test whereby dehydrogenase enzymes in the mitochondria of viable cells have the capacity to reduce colourless TTC to red formazan crystals in growth media. The calorimetric change is directly proportional to the rate of respiratory activity in cells. The results in Fig. 6 substantiate proteomics findings, showing a 36-69% reduction in respiration following exposure to SBC3 at 15-75 μg/ml.

Assessment of the effects of SBC3 on C. parapsilosis adherence and biofilm formation
Proteomic analysis of cells exposed to SBC3 indicated alterations in the abundance of proteins associated with cell wall synthesis ( chitinases ) , adherence, and biofilm formation ( Candida_ALS_N domain-containing protein, CFEM2 and 5/6 ) ( Fig. 5 , Supplementary File 1 ) , and ergosterol biosynthesis ( encoded by ERG4 , ERG11, and NCP1 ) ( Fig. S2 ) . The effects of SBC3 on adherence of C. parapsilosis to buccal epithelial cells ( BECs ) and biofilm formation on an abiotic surface were characterized to ascertain whether the proteomic alterations had an impact on the cell's disease-causing potential. Candida parapsilosis grown with SBC3 for 24 h was isolated and co-incubated with BECs for 120 min to permit the attachment of cells. Adhered yeast cells were enumerated on each BEC for each treatment group, revealing pre-treatment with SBC3 ( 15, 25, 50, and 75 μg/ml ) resulted in a 76.6 ± 15.5% reduction in adherence ( Fig. 7 A ) .
To assess the effect of SBC3 on biofilm formation, C. parapsilosis cells were cultured in the presence of SBC3 ( 10, 15, 20, 25, 50, and 75 μg/ml ) for 48 h. The growth of cells was initially measured to verify biofilm reduction was a direct result of SBC3 treatment rather than reduced cell presence. Post-removal of planktonic cells, the remaining adhered cells were stained and measured spectrophotometrically. The results showed a dosedependent reduction in biofilm formation by 40-76%, while no reduction in cell growth was observed for all test concentrations ( Fig. 7 B ) .
SEM was employed to view structural and morphological changes in C. parapsilosis , further supporting the antibiofilm capabilities of SBC3 ( Fig. 8 ) . A dense network of cells in both yeast ( oval and singular ) and pseudohyphal ( elongated and attached ) forms was evident in untreated control samples ( Fig. 8 A and C ) . Filamentous cells layered on top of aggregated cells in the yeast form are adhered to the slide surface, aided by extracellular matrix ( ECM ) material ( Fig. 8 E ) . To observe the full potential of the complex, the highest test dose of SBC3 ( 75 μg/ml ) was selected to treat cells. As a result, cells appeared more dispersed, with a reduced presence of biofilm ( and ECM material ) ( Fig. 8 B ) and were predominantly in the yeast form with a rounded morphology ( Fig. 8 D ) and rough texture characteristic of cell wall damage shown in Fig. 8 F. 17

Discussion
While C. albicans remains a significant fungal pathogen, the epidemiology of Candida infections has altered over recent years with increasing reports of C. parapsilosis infections. 11 Incident rates are likely to increase with the ongoing use of aggressive immunosuppressive therapies and invasive medical procedures, despite limited treatment options. 48 Much of C. parapsilosis virulence is due to its adherence ability and biofilm formation. 49 Silver research has largely focused on the antibacterial activity of silver despite demonstrating promising antifungal properties. The activity of silver against bacteria and fungi appears largely universal, having the capacity to induce ROS production. During normal cell activity, viable cells produce low levels of ROS in the mitochondria that are modulated with antioxidants. Silver treatment can perturb this homeostasis via the binding of enzymes in the respiratory chain, resulting in oxidative damage to nucleic acids, proteins, and lipids. Silver can also contribute to structural damage. Association with cell membranes/walls and formation of pores alters permeability, resulting in ion leakage, and ultimately, cell death. 50 -53 Studies have also reported the inhibition of filamentation, and hence biofilm formation, in fungi, while Kim et al . demonstrated inhibition of growth via cell cycle arrest and prevention of cell budding in C. albicans in response to silver. 17 , 22 Quantitative label-free proteomics were employed to assess the effect of SBC3 on the proteome of SBC3-treated C. parapsilosis cells and identified considerable increases in the abundance of chitinase. Chitin is a polysaccharide component of the fungal cell wall that provides structural stability to the cell. 54 Cell growth relies on an interplay between biosynthetic chitin enzymes and chitin degrading enzymes ( chitinases ) for morphological changes and cell turnover. 55 , 56 SBC3 induced an increased abundance of chitinase, encoded by CPAR2_502120 / CPAR2_502130 . In addition, several CFEM proteins ( 2, 5-7 ) were significantly more abundant in both SBC3-treated sample groups. The CFEM domain comprises eight conserved cysteine residues belonging to a group of proteins specific to fungi. 57 CFEM proteins function in adherence, biofilm formation, and pathogenicity, with other suspected functions as signal transducers and cell-surface receptors. 58 , 59 It is believed that CFEM proteins influence biofilm formation in C. parapsilosis, although their exact effect has not been fully elucidated. 60 We hypothesize the elevated levels of the aforementioned proteins are a compensatory mechanism to withstand the effects of SBC3 in the disruption of cell wall integrity and biofilm formation.
ATP-binding cassette ( ABC ) transporter proteins act as efflux pumps for xenobiotics, drugs, and metals, and are encoded by CDR1 and CDR2 genes. 61 , 62 Resistance typically coincides with altered expression of multiple genes, where C. parapsilosis showed reduced susceptibility to fluconazole from increased expression of CDR1 and ERG11 . 63 SBC3 reduced expression of the ergosterol synthesis gene ERG11 while ERG4 was increased in treated samples in combination with NCP1 -encoded NADPH cytochrome P450 reductase required for cell detoxification. 64 The link between increased ERG4 and azole resistance remains, unclear although significantly increased levels of ERG4 mRNA have previously been detected in azole resistant C. albicans compared to susceptible isolates. 65 Ergosterol is a lipid component of biological membranes, which maintains structure and permeability of the cell and therefore, these alterations in the cell membrane may possibly reveal a method of cellular detoxification and physical defense against environmental stress. 66 , 67 Evidence of cellular stress response is further revealed with an increased abundance of oxidoreductases. Thioredoxin plays a crucial role in detoxifying peroxides and the flavoenzyme thioredoxin reductase catalyses this process to maintain cell redox homeostasis and protect against oxidative stress. 68 , 69 While studies have presented possibilities of thioredoxin reductases as antifungal targets, sub-inhibitory doses of SBC3 significantly elevated levels of thioredoxin domain-containing protein. 68 , 70 , 71 The most apparent change in SBC3-treated proteomes was the decrease in the abundance of proteins associated with protein synthesis. A large proportion of protein clusters generated on Cytoscape ( Fig. S2 ) contain translation/elongation factors, tRNA synthetases, ribosomal constituents, along with ribosome biogenesis, RNA binding, and rRNA processing proteins. Ribosomes are complex macromolecules that facilitate amino acid linkage to generate peptide chains, and the assembly of ribosomes is an intricate process of rRNA transcription, folding, and coupling of 60S and 40S subunits. 72 -74 Ribosomal protein L37 contributes to early ribosome assembly and demonstrated the greatest change ( -49-fold ) among decreased proteins in C. parapsilosis exposed to 25 μg/ml SBC3. 75 Ribosome assembly, and indeed protein synthesis, are essential to sustain normal cell growth, and this vulnerability has been exploited in novel and existing drug targets. Sun et al . suggested a similar mode of action for a repurposed formulation of a thiosemicarbazone via transcriptional analysis. 76 A novel sordarin derivative has shown specific action against the protein synthesis elongation cycle in some Candida spp. and bears no adverse effect on mammalian protein synthesis. 77 Amino acids are integral components of proteins, and synthesis and metabolism require strict control for adequate protein formation and nutrient acquisition. 78 , 79 In the yeast Saccharomyces cerevisiae , amino acid production relies on feedback regulation of biosynthetic enzymes encoded by ILV genes. 78 The data presented here identified decreased expression of these genes listed as follows with their protein products: ILV1 ( threonine dehydratase ) , ILV2 ( acetolactate synthase ) , ILV3 ( dihydroxy-acid dehydratase ) , and ILV5 ( ketol-acid reductoisomerase ) . In addition, proteins required for histidine biosynthesis ( ATP phosphoribosyltransferase, histidine biosynthesis trifunctional protein, and imidazole glycerol phosphate synthase hisHF ) , leucine biosynthesis ( pyruvate carboxyltransferase domain-containing protein ) , lysine biosynthesis ( saccharopine dehydrogenase and alpha-aminoadipate reductase ) , and tryptophan biosynthesis ( anthranilate synthase ) were also found within downregulated clusters. Pathogenic fungi employ efficient strategies of nutrient assimilation to support cell growth and various virulence traits ( including biofilm formation ) , and to combat environmental stresses. 80 - 82 Amich and Bignell outlined a selection of fungal genes necessary in pathogenesis, such as ILV , HIS , TRP , and LEU , which encode the proteins mentioned earlier. 83 Furthermore, the potential of amino acid synthesis/metabolism disruption as a novel antifungal target has been summarized 84 -86 For example, inhibition of the leucine biosynthetic enzyme isopropylmalate dehydrogenase can attenuate virulence in Cryptococcus neoformans and phytopathogenic Magnaporthe spp., 84 , 87 , 88 and methods of 3-isopropylmalate dehydratase inhibition for use in fungicides have even been patented. 84 The most significantly decreased protein in response to SBC3 was 3isopropylmalate dehydratase. These biosynthetic enzymes have proven attractive antifungal targets due to their absence in mammals, which must obtain essential amino acids from the diet. 86 , 89 The ability of Candida spp. to adapt to hostile environments is a complex, multifactorial process dependent on adequate energy supplies for the maintenance of growth and metabolism. 90 , 91 Components of cytochrome c ( cytochrome b-c1 complex subunit 7, cytochrome b-c1 complex subunit Rieske, cytochrome c domain-containing protein encoded by CYC1 ) and a cytochrome c oxidase ( COX ) subunit, encoded by COX4 , were decreased in abundance. The latter protein catalyses the final step of the electron transport chain and regulates oxidative phosphorylation for ATP synthesis. 92 -94 Furthermore, SBC3 significantly altered the abundance of NADH dehydrogenase [ubiquinone] flavoprotein 1 for NADH electron transfer to the respiratory chain ( -25-fold ) . Other mitochondrial proteins significantly decreased in abundance are listed in Table S1.

Paper | 7
Mitochondrial function extends beyond energy production to involvement in virulence and resistance mechanisms. 95 , 96 Defective ATP-generating components of the mitochondria are capable of inhibiting C. albicans 's adherence and biofilm formation, 97 while reduced respiration in phenazine-treated C. albicans achieved similar results. 98 SBC3 ( 15 and 25 μg/ml ) decreased Candida_ALS_N domain-containing protein ( encoded by CPAR2_404800 ) by 9and 5-fold, respectively ( Supplementary File 1 ) . Agglutinin-like sequence ( Als ) proteins are a well-established family of adhesins which initiate microbial colonization to biotic and abiotic surfaces. 99 Pre-treatment of C. parapsilosis with SBC3 reduced yeast adherence to BECs by up to 92%, complementing previous studies by Bertini et al ., which showed over 60% reduced adhesion to BECs in C. parapsilosis CPAR2_404800 null mutants. 100 SBC3 exposure can also reduce biofilm formation, which is an important virulence factor of C. parapsilosis . 101 Biofilm visualization by SEM revealed a decreased presence of ECM in SBC3-treated cells. Comprised of polysaccharides, lipids, DNA, and proteins, ECM aids cell adhesion, offers an impenetrable barrier against antifungal agents and a nutrient source to cells. 102 , 103 Furthermore, SBC3 appeared to hinder the transition from yeast to pseudohyphal forms. A number of genes are expressed during this shift, including many cell adhesion proteins, and are likely to explain the reduced expression of Candida_ALS_N domain-containing protein. 104 Adherence to host tissue and biofilm formation are essential for the initial stages of infection. The ability of SBC3 to reduce these processes along with morphological shifts would adversely affect the ability of C. parapsilosis to colonize and disseminate in the host .
LFQ MS offers a powerful approach to studying cell proteomes and permit the identification of changes within complex sets of proteins and associated networks/pathways. Therefore, it has been applied to provide insight on the effect ( s ) of novel and conventional antifungal treatments. 105 -108 The results presented here indicate that exposure of C. parapsilosis to SBC3 results in reductions in proteins associated with protein synthesis, amino acid synthesis, and respiration and in virulence as measured by the inhibition of adherence and biofilm formation. These findings provide a novel insight into the mode of action of SBC3 and encourage further research into this interesting antifungal agent.

SBC3 complex synthesis
SBC3 was synthesized as previously described. 47

SBC3 toxicity evaluation
SBC3 was dissolved in YEPD and 5% DMSO ( Merck ) and serially diluted in 96-well plates ( Corning ) to final concentrations of 0.49-125 μg/ml. Aliquots ( 100 μl ) of C. parapsilosis cultures grown overnight were diluted in sterile phosphate buffered saline ( PBS ) and added to serially diluted complexes to obtain a final optical density ( OD ) of 0.05 ( representing ∼ 4.5 ×10 5 cells/ml ) . Plates were incubated at 30°C for 24 h in a static incubator. Growth was measured in a plate reader ( Bio-Tek Synergy HT ) at 600 nm.

Candida parapsilosis protein extraction and purification
Sterile YEPD media supplemented with SBC3 ( 15 and 25 μg/ml ) was inoculated with C. parapsilosis , and cells were grown for 8 h at 30°C in an orbital shaker at 120 rpm ( n = 3, per sample group ) . Cells were washed three times in sterile PBS, resuspended in lysis buffer ( pH 8.0 ) containing 6 M urea ( Sigma ) , 2 M thiourea ( Sigma-Aldrich ) , 0.1 M Tris-HCl ( Sigma ) and a range of protease inhibitors [10 μg/ml aprotinin ( Caymen Chemical Company ) , leupeptin ( Thermo Scientific ) , pepstatin A ( Sigma ) , Tosyllysine Chloromethyl Ketone hydrochloride ( TLCK ) , and 1 mM/ml phenylmethylsulfonyl fluoride ( PMSF; Sigma ) ], and lysed via sonication. Cell debris was pelleted by centrifugation at 13 000 x g for 5 min. Proteins were acetone ( Sigma ) precipitated in a ratio of 1:3, sample to acetone, overnight at -20°C. The acetone was discarded, and proteins were resuspended in 25 μl of resuspension buffer ( same as lysis buffer without protease inhibitors ) . Aliquots ( 2 μl ) of samples were used to quantify proteins with the Qubit TM quantification system ( invitrogen ) . To the remaining samples, 105 μl of 50 mM ammonium bicarbonate and 1 μl 0.5 M dithiothreitol ( Sigma-Aldrich ) were added, and the samples were incubated at 56°C for 20 min. Samples were alkylated with 2.7 μl 0.5 M iodoacetamide ( Sigma-Aldrich ) and incubated in the dark at room temperature for 15 min. Proteins were digested with 1 μl 1% ( w/v ) Pro-teaseMAX and 1 μl 0.5 μg/ μl Sequence Grade Trypsin ( Promega ) overnight at 37°C. Digestion was inhibited with 1 μl 100% trifluoroacetic acid ( Sigma-Aldrich ) . Samples were incubated at room temperature for 5 min and centrifuged at 13 000 x g for 10 min. Peptides in the resulting supernatant were purified using C-18 spin columns ( Pierce ) and dried in a SpeedyVac concentrator ( Thermo Scientific Savant DNA120 ) at 39°C for approximately 2 h. Samples were resuspended in 2% acetonitrile and 0.05% TFA, sonicated in a water bath for 5 min and centrifuged at 13 000 x g for 5 min. The supernatant was used for mass spectrometry analysis.

Mass spectrometry
Purified samples ( 2 μl containing 0.75 μg protein ) were loaded onto a Q Exactive Mass Spectrometer ( ThermoFisher Scientific ) using a 120-min reverse phase gradient as per previous methods. 109 Raw MS/MS data were processed through the Andromeda search engine in MaxQuant software v.1.6.3.4 110 using a C. parapsilosis database obtained from a UniProt-SWISS-PROT database to identify proteins ( 5777 entries, downloaded February 2021 ) . Search parameters were followed as per previous methods. 111

2,3,5-Triphenyltetrazolium chloride assay
Candida parapsilosis was grown in SBC3-supplemented media for 12 h at 30°C in an orbital shaker. Cells were harvested, washed twice with PBS, and resuspended accordingly to standardize the ODs of all samples to 0.15. A stock solution of TTC made in PBS was filter sterilized using a 0.22 μm pore filter and added to cell suspensions at a 1:10 dilution. The solutions were added to wells in a 96-well plate in 100 μl aliquots containing 0.5 mg/ml TTC per well and incubated for 8 h at 30°C in a static incubator. The supernatant was removed, and the remaining formazan crystals were solubilized with 100% DMSO for 15 min in an orbital shaker. The absorbance was measured at 550 nm.

Adherence assay
Candida parapsilosis was grown in YEPD media containing 15, 25, 50, and 75 μg/ml SBC3 at 30°C for 24 h. Cells were harvested, resuspended in PBS, and enumerated. BECs were self-isolated by gently harvesting from the inside cheek of a healthy individual using a tongue depressor. Yeast cells and BECs were combined in a ratio of 50:1, yeast: BEC, in PBS to a final volume of 2 ml. The cell mixture was incubated at 30°C for 120 min at 120 rpm. The solution was passed through a polycarbonate membrane with 30 μm pores and washed with 8 ml of PBS to eliminate non-adhered C. parapsilosis cells. The remaining BECs with attached C. parapsilosis cells were applied to microscope glass slides that were dried overnight, heat fixed, and stained with 0.5% crystal violet dye ( ACROS Organics ) for 1 min. The average number of adhering yeast cells per BEC was determined after counting 100 BEC per treatment.

Biofilm assay
Candida parapsilosis was cultured overnight, diluted in YEPD medium and aliquoted [90 μl of OD 0.1 ( ∼ 9 ×10 5 cells/ml ) ] into flat-bottom 96-well plates ( Corning ) . A range of SBC3 concentrations were added in 10 μl aliquots. Plates were incubated at 30°C for 48 h in a static incubator. Growth was measured as previously described. Planktonic cells were removed, and plates were washed four times with deionized water. Adhered cells were stained with 0.1% crystal violet dye for 15 min. The dye was removed, and plates were washed to remove excess. The remaining stain was air dried overnight and solubilized with 100 μl, 30% acetic acid ( Fisher ) . The intensity of staining was measured at 550 nm.

Scanning electron microscopy
Candida parapsilosi s cell suspensions ( OD 0.1 ) treated with SBC3 were grown in 6-well plates containing glass slides for 48 h at 30°C in a static incubator. SBC3 solutions were made with DMSO and YEPD. Control samples consisted of YEPD and 0.375% DMSO as per treated samples. The supernatants were removed from wells and the remaining adhered cells on glass slides were fixed with 2.5% glutaraldehyde ( Sigma ) for 2 h. Cells were washed with prewarmed PBS and dehydrated in increasing ethanol solutions ( 35%, 50%, 70%, 80%, 90%, and twice in 100% ) for 10 min each. Slides were dried with 100% hexamethyldisilazane ( Sigma-Aldrich ) for 10 min followed by overnight air-drying. Slides were sputtered with gold ( 6-12 nm ) and imaged on a scanning electron microscope ( HITACHI S-3200N ) .

Data analysis
Graphs were constructed and analysed on GraphPad Prism v.9.2.0. Statistical analysis via one-way ANOVA with Tukey's multiple comparisons test was performed on the means of all sample groups.
The resulting LFQ intensity values were processed through Perseus v.1.6.6.0 ( www.maxquant.net/) to generate statistical and graphical analysis. 112 Normalized LFQ intensity values were used as protein abundance measurements. Proteins were filtered to remove contaminants and peptides identified by site. LFQ intensities were log 2 transformed, and samples were grouped accordingly ( control, 15 μg/ml treatment, and 25 μg/ml treatment ) . Proteins not found in three out of the three replicates in at least one group were removed. The data were imputated to replace missing values with values that imitate signals of low abundance proteins randomly selected from a distribution designated by a downshift of 1.8 times the mean standard deviation of all measured values and a width of 0.3 times this standard deviation. Comparisons between two sample groups were conducted via pair-wise Student's t -tests with an FDR cut-off of 0.05 on post-imputated data. Vol-cano plots were produced by plotting log 2 fold changes on the x axis and -log P -values on the y axis for each sample group comparison. SSDA proteins [ANOVA, P < 0.05, fold change ≥1.5] were used in subsequent analyses. LFQ intensities were z -score normalized for hierarchal clustering of the median expression values of SSDA proteins using Euclidean distance. A Fisher's Exact test ( Benjamini-Hochberg, FDR cut-off of 5% ) was used to perform GO term enrichment analysis of major protein clusters for enrichment in GO biological process, GO cellular component, and GO molecular function. The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD025693. 113 Cytoscape v.3.8.2 bioinformatics software was used to map SSDA proteins retrieved from UniProt gene lists in Perseus. A high confidence score ( 0.9 ) was used to generate interaction networks of up-and downregulated protein pathways for treatment versus control groups. Functional enrichment analysis was performed on protein clusters for enrichment in UniProt key words to identify and annotate functions/processes.

Supplementary material
Supplementary data are available at Metallomics online.

Funding
The production of this publication was supported by funding from a research grant from Science Foundation Ireland ( SFI ) and is cofunded under the European Regional Development Fund under grant number 12/RC/2275_P2. Q Exactive mass spectrometer was funded under the SFI Research Infrastructure Call 2012; Grant Number: 12/RI/2346 ( 3 ) .

Conflicts of interest
There are no conflicts to declare.

Data availability
The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD025693. 113