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TLC, GLC and MS of complex lipid mixtures from uropygial secretions.

A general technique of investigation of uropygial gland secretions is described including sample collection and extraction as well as partition, TLC and column chromatography of the crude lipids. The main components, generally ester waxes, are cleaved by CH3OH/HCl followed by conversion into fractions ready for GLC. Some GLC and MS details are reported.