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Investigation of the potential association between the levels of global DNA methylation, vitamin B12 and type 2 diabetes in Iraqi patients

Type 2 diabetes mellitus (T2DM) is an epidemic metabolic disorder that has life threatening health complications if left undetected and untreated. Understanding its molecular landscape alterations, especially DNA methylation that regulates the genome transcription activity, would aid with the prevention and management of this disease. This study was set to investigate the global DNA methylations (assessed by measuring 5mC level), along with other disease-related clinical features, including body mass index (BMI) and serum vitamin B12, in T2DM patients in comparison to healthy controls. One hundred subjects (70 T2DM patients and 30  healthy controls) who attended Baghdad Teaching  Hospital/ Department of Consulting Clinic - Endocrinology and Diabetes Consultant and the National Diabetes Center, Al-Mustansiriyah University, Baghdad, Iraq. Global DNA methylation levels were assessed using Methyl flash ™ Global DNA methylation (5-mC) ELISA Easy Kits. The results showed significant increase (P=0.0018) in the 5mC level of T2DM patients in comparison to the healthy subjects (0.578±0.208 vs. 0.422±0.110, respectively). 