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Multiparametric Analysis of Tissue Spheroids Fabricated from Different Types of Cells.

Reproducible, scalable and cost effective fabrication and versatile characterization of tissue spheroids is highly demanded by 3D bioprinting and drug discovery. Consistent geometry, defined mechanical properties, optimal viability, appropriate ECM/cell organization are required for cell aggregates aimed for application in these fields. We suggest a straightforward procedure for fabrication and systematic multiparametric characterization of tissue spheroids with defined properties and uniform predictable geometry employing non-adhesive technology. Applying immortalized and primary cells, we have demonstrated the reproducibility of spheroid generation, the strong correlation of ultimate spheroid diameter and growth pattern with cell type and initial seeding concentration. Spheroids viability and mechanical properties were governed by cell derivation. In our study, we suggest a new decision procedure to apply for any cell type one starts to work with to prepare and typify tissue spheroids meeting high quality standards in biofabrication and drug discovery. This article is protected by copyright. All rights reserved.