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Optimization of Fermentation Reaction Conditions for Preparation of PATE Enzyme by Erwiniacarotovora IFO3830 by TFCCRD Method

The fermentation conditions for preparation of polygalacturonic acid transeliminase (PATE) enzyme by Erwinia carotovora IFO3830 were optimized for seed ratio, vibration rate, and temperature by the TFCCRD method. The results indicated that the optimum fermentation conditions for E. carotovora IFO 3830 were that seed ratio, vibration rate, and temperature were 5% v/v, 113 min−1, and 29 °C, respectively.