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Independent Sets of DNA Oligonucleotides for Nanotechnology Applications

Independent sets of DNA oligonucleotides, which only bind with their Watson-Crick complements, have potential use in self-assembly of nanostructures, since they minimize errors and inefficiency from unwanted binding. A software tool implemented a thermodynamic model for DNA duplex formation and was used to generate large independent sets of DNA oligonucleotides. The principle of the approach was experimentally verified on a sample set of oligonucleotides.