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The Characterization of a Class of RNA in HeLa Cytoplasm Derived from 9185 RNA

A class of RNA in HeLa cytoplasm with a sedimentation value of 22S is described . This RNA is a true cytoplasmic component and is not an artifact of cell rupture or of the method of RNA preparation. The 22S RNA sediments in a sucrose gradient with those ribosomal structures containing 28S ribosomal RNA (60S and 74S particles and polyribosomes) . It has a base composition and methyl content similar to those of 28S RNA. The kinetics of formation of 22S suggest that it is not a direct product of transcription but is derived in vivo from "old" molecules of 28S RNA .