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miR-320 inhibits human coronary artery smooth muscle cell proliferation and migration by targeting Krüppel-like factor 5

Krüppel-like factor 5 (KLF5) plays an essential role in the regulation of vascular smooth muscle cell proliferation and migration. In the present study, a post-translational mechanism of miR-320 in TNF-α-induced proliferation and migration of HCASMCs by targeting KLF5 was investigated. The results indicated that TNF-α could accelerate the proliferation and migration of HCASMCs. Overexpressed miR-320 could suppress the proliferation and migration of HCASMCs in the absence and presence of TNF-α. Moreover, bioinformatic analysis showed the potential miR-320 binding sites within the 3’-UTR of KLF5. KLF5 as a direct target of miR-320 was involved in TNFα-induced proliferation and migration of HCASMCs. KLF5 knockout could reverse the increased proliferation and migration in HCASMCs induced by miR-320 loss-of-function and TNF-α treatment. In conclusion, overexpressed miR-320 was verified to protect against TNF-α-induced proliferation and migration of HCASMCs, and the underlying mechanism was mediated, at least partially, by inhibiting KLF5 expression.