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THE ANKYRIN REPEAT-RICH MEMBRANE SPANNING (ARMS)/KIDINS220 SCAFFOLD PROTEIN IS REGULATED BY ACTIVITY-DEPENDENT CALPAIN PROTEOLYSIS AND MODULATES SYNAPTIC PLASTICITY

The expression of forms of synaptic plasticity, such as the phenomenon of long-term potentiation, requires the activity-dependent regulation of synaptic proteins and synapse composition. Here we show that ARMS (ankyrin repeat-rich membrane spanning protein)/Kidins220, a transmembrane scaffold molecule and BDNF TrkB substrate, is significantly reduced in hippocampal neurons after potassium chloride depolarization. The activity-dependent proteolysis of ARMS/Kidins220 was found to occur through calpain, a calcium-activated protease. Moreover, hippocampal long-term potentiation in ARMS/Kidins220(+/-) mice was enhanced, and inhibition of calpain in these mice reversed these effects. These results provide an explanation for a role for the ARMS/Kidins220 protein in synaptic plasticity events and suggest that the levels of ARMS/Kidins220 can be regulated by neuronal activity and calpain action to influence synaptic function.

The expression of forms of synaptic plasticity, such as the phenomenon of long-term potentiation (LTP), requires the activitydependent regulation of synaptic proteins and synapse composition. Here we show that ARMS/Kidins220 (Ankyrin Repeat-rich Membrane Spanning protein), or Kidins220 (KInase D-INteracting Substrate of 220 kD), a transmembrane scaffold molecule and BDNF-TrkB substrate, is significantly reduced in hippocampal neurons after potassium chloride depolarization.
The activity-dependent proteolysis of ARMS/Kidins220 was found to occur through calpain, a calcium-activated protease. Moreover, hippocampal LTP in ARMS/Kidins220 +/mice was enhanced, and inhibition of calpain in the mice reversed these effects. These results provide an explanation for a role for the ARMS/Kidins220 protein in synaptic plasticity events and suggest that the levels of ARMS/Kidins220 can be regulated by neuronal activity and calpain action to influence synaptic function.
Activity-dependent changes in protein function and composition are known to accompany changes in synaptic efficacy. Such processes are frequently mediated by calcium signaling and have been well studied in the synaptic plasticity process of long-term potentiation (LTP). Activity-induced synaptic changes can occur through upregulation of proteins via novel gene transcription (1). Synaptic proteins can also undergo regulated turnover or degradation through proteasome- (2) or calpainmediated proteolysis (3,4).
One molecule that may play a role in synaptic plasticity is the Ankyrin Repeat-rich Membrane Spanning (ARMS) protein, or the KInase D-INteracting substrate of 220 kD (Kidins220). Initially identified as an interactor of Trk and p75 neurotrophin receptors (17), as well as a protein kinase D substrate (18), ARMS/Kidins220 is a transmembrane scaffold protein that is highly expressed in the nervous system. Strikingly, it is phosphorylated by Trk and Eph receptors, but not by other growth factor tyrosine kinase receptors such as the EGF receptor (17). Therefore, ARMS/Kidins220 represents a receptor tyrosine kinase substrate that lends signaling specificity to certain neuronal pathways (17,19). In the central nervous system, the TrkB receptor phosphorylates ARMS/Kidins220 after brain-derived neurotrophic factor (BDNF) binding. Since BDNF and TrkB are highly responsive to neuronal activation, we hypothesized that downstream signal transducers like ARMS/Kidins220 might also be involved in activity-dependent events as a mechanism for facilitating synaptic plasticity.
Here we show that the calcium-activated protease calpain is responsible for degrading ARMS/Kidins220 after neuronal activation. In vivo, genetic reduction of ARMS/Kidins220 levels leads to enhanced LTP, and this effect of ARMS/Kidins220 on LTP is dependent on calpain. These data indicate that ARMS/Kidins220 levels can be regulated by neuronal activity through calpain proteolysis, which have consequences upon hippocampal synaptic plasticity.
The purified His-tagged N-terminal fragment (1-402 aa) of ARMS/Kidins220 was used for immunization of two rabbits by Biogenes GmbH following standard procedures. Affinity-purified antibodies were isolated using antigen-specific CNBr-Sepharose columns and tested for immunoreactivity. The antibody was used in Western blots at a dilution of 1:200. Primary hippocampal cultures -Primary hippocampal neurons were dissected from E18-19 Sprague Dawley rat embryos in Ca 2+ -and Mg 2+free HBSS (Gibco) supplemented with 0.37% glucose, digested with 0.05% trypsin, mechanically dissociated with fire-polished Pasteur pipettes, and plated in poly-D-lysinecoated 12-well plates at 250,000 neurons per well. Cells were grown in Neurobasal media (Gibco) supplemented with B27 supplement (Gibco), 0.37% glucose, 0.5 mM glutamine (Gibco), and 1.2 µg/ml 5-fluoro-2-deoxyuridine. Fresh media was added to the cells every 3-4 days.
Cells were stimulated at DIV 14-21 (days in vitro) with 50 mM KCl or 200 µM glutamate. For KCl treatments, cells were collected at timepoints after the addition of 50 mM KCl to the cell media. For glutamate treatments, 200 µM glutamate was added to the media for one minute, then the media was completely replaced with conditioned media without glutamate. Cells were collected at timepoints after the removal of glutamate. When pharmacological inhibitors were used, they were applied to the cells 30 minutes prior to and were maintained throughout the stimulation. Western blots -Cells were collected using 80 µl 1X SDS buffer per well of each 12-well plate and boiled for 5 minutes. 12 µl of sample was separated on SDS-PAGE gels, transferred to PVDF membranes (Millipore), and incubated with primary and HRP-conjugated secondary antibodies. Immunoreactive proteins were visualized by ECL detection (Amersham Biosciences) and film autoradiography. Band intensities were quantified using ImageJ software, and statistical analysis was performed using Graphpad Prism software. All results were obtained from at least three independent experiments. Electrophysiology in ARMS/Kidins220 +/mice -ARMS/Kidins220 +/mice were previously described (20). Hippocampi were dissected from 3-to 6-month-old male ARMS/Kidins220 +/mice and wild-type littermates and cut into 400-mm transverse slices with a tissue chopper (EMS). Slices were incubated in an interface chamber at 29ºC for 90 min before recording, where they were subfused with saline (ACSF) consisting of 124 mM NaCl, 4.4 mM KCl, 1.0 mM Na2HPO4, 25 mM NaHCO3, 2.0 mM or 2.5 mM CaCl2, 2.0 mM or 1.3 mM MgSO4, and 10 mM glucose and bubbled with 95% O2 and 5% CO2. A bipolar tungsten stimulating electrode and a glass micropipette (5-10 MΩ, filled with ACSF) recording electrode were placed the stratum radiatum in the CA1 region, and extracellular field potentials were recorded. Basal synaptic transmission was assayed by plotting the stimulus voltages (V) against the slopes of the fEPSPs to generate input-output relations. For LTP experiments, baseline stimulation was delivered every min for 15 min at an intensity that evoked a response approximately 35% of the maximum evoked response. LTP was induced using a θ-burst stimulation of four pulses at 100 Hz, with bursts repeated at 5 Hz and each tetanus including three ten-burst trains separated by 15 sec. In experiments using calpain inhibitor BDA-410, slices were perfused with the inhibitor (100 nM) 20 min before LTP induction.

LTP is enhanced in ARMS/Kidins220 +/mice.
We previously reported a line of mice heterozygous for the ARMS/Kidins220 gene that displays a 30-40% reduction of ARMS/Kidins220 protein compared to wild-type animals (20). Homozygous mutants die at an embryonic age. To examine the effect of ARMS/Kidins220 reduction on synaptic plasticity, acute hippocampal slices were taken from 3-to 6-month-old ARMS/Kidins220 +/mice, and electrophysiology measurements were performed. LTP at the Schaffer collateral-CA1 synapse was induced using a θ-burst stimulus, and the slopes of the field excitatory postsynaptic potentials were recorded. Strikingly, in ARMS/Kidins220 +/mice LTP was enhanced compared to wild-type animals ( Fig. 1A; F1,17 = 5.084, p < 0.05, two-way ANOVA). ARMS/Kidins220 +/mice also exhibited increased synaptic transmission at lower stimulus intensities (Fig. 1B). These data indicate that levels of ARMS/Kidins220 in vivo regulate synaptic transmission and synaptic plasticity.
ARMS/Kidins220 is degraded after neuronal activation by KCl depolarization. It was previously shown that ARMS/Kidins220 protein levels respond to long-term changes in neuronal activity (21). When the sodium channel blocker TTX was used to inhibit hippocampal cultures over a course of 48 hours, ARMS/Kidins220 protein levels increased, and when the GABA receptor inhibitor bicuculline was used to increase activity in cultures, ARMS/Kidins220 protein levels decreased. Since such treatments have been shown to induce homeostatic changes in neurons (22), these data suggest that ARMS/Kidins220 acts as an activity sensor with a possible role in synaptic plasticity.
To study the regulation of ARMS/Kidins220 due to acute neuronal activation, we depolarized rat hippocampal cultures with 50 mM potassium chloride (KCl), a stimulus that is widely used to study activity-dependent neuronal changes (23-25). Cultures were treated with KCl, and lysates were collected in a timecourse and probed by Western blot for ARMS/Kidins220 protein using an antibody directed against the C-terminus (17) and one directed against the N-terminus.
Neuronal activation caused a reduction in ARMS/Kidins220 levels to about 50% of basal levels within three hours as a result of protein degradation ( Fig. 2A and B). The N-terminus antibody not only showed degradation of the fulllength 220 kD ARMS/Kidins220 protein, but it also detected smaller-sized degradation products at about 140, 120, and 85 kD. These were present at low levels in untreated cultures and accumulated with prolonged neuronal activation ( Fig. 2A,  labeled a, b, and c). Addition of the calcium chelator EGTA to the culture media prevented KCl-induced degradation of ARMS/Kidins220, indicating extracellular calcium influx after membrane depolarization is required ( Fig. 3A and B). Taken together, these data indicate that KClinduced neuronal activation causes calciummediated degradation of full-length ARMS/Kidins220 to smaller-sized fragments.
Glutamate-induced neuronal activation causes ARMS/Kidins220 degradation. In addition to KCl, glutamate, the most prominent excitatory neurotransmitter in the nervous system, is commonly used to activate cultured neurons to induce physiologic synaptic activity (26). To demonstrate that activity-induced ARMS/Kidins220 degradation is not restricted to KCl depolarization, we assayed ARMS/Kidins220 protein levels after glutamate treatment of hippocampal cultures. Similar to the effect of KCl, full-length ARMS/Kidins220 was degraded when neurons were activated by glutamate (Fig. 4A). Additionally, the same N-terminal degradation products also were detected (Fig. 4A, arrows a, b, and c). The degradation of ARMS/Kidins220 and the accumulation of the smaller fragments were prevented by the addition of EGTA (Fig. 4B).
These results indicate that ARMS/Kidins220 degradation can result from multiple forms of neuronal activation, including glutamate-induced activation.
ARMS/Kidins220 degradation is independent of proteasomes or lysosomes. Activity-dependent turnover of proteins at the PSD is a mechanism for protein composition regulation at the highly dynamic synapse (27). Proteasomal activity is implicated in the turnover of PSD proteins (2) and can be regulated by BDNF (28). We first hypothesized that the degradation of ARMS/Kidins220 after neuronal activity was due to the action of proteasomes. Inhibitors of proteasomes were used to determine if they could prevent ARMS/Kidins220 degradation. Incubation of hippocampal cultures with the proteasomal inhibitors epoxomycin or lactacystin during KCl stimulation did not attenuate ARMS/Kidins220 degradation, indicating that proteasomal digestion was not the primary mechanism of degradation ( Fig. 5A and C).
Another degradation pathway is through lysosomes, where hydrolases digest proteins in acidic compartments after internalization from the cell membrane. Since ARMS/Kidins220 is a transmembrane protein, we tested whether blocking lysosomal action using chloroquine, which deacidifies lysosomes and inactivates lysosomal proteases, could block ARMS/Kidins220 degradation. Incubation of hippocampal cultures with chloroquine during KCl stimulation had no significant effect on ARMS/Kidins220 degradation, suggesting that the degradation did not occur through the lysosomal pathway ( Fig. 5B and C).
ARMS/Kidins220 degradation is due to calpain proteolysis. Alternatively, activity-induced proteolysis of ARMS/Kidins220 could occur through the action of proteases that are activated by calcium. One potential candidate is calpain. The two major isoforms of calpain, µ-and mcalpain, are highly expressed in neurons and respond to micro-and millimolar concentrations of intracellular calcium, respectively, in part through the binding of calcium directly to domains in the molecule (5). Calpain acts at neutral pH in the cytosol and at the cell membrane to cleave a wide range of targets. Furthermore, calpain activity has been implicated in synaptic functions and neurodegenerative diseases (3,4).
To test whether calpain degrades ARMS/Kidins220, hippocampal cultures were incubated with the calpain inhibitor MDL28170 or ALLM during KCl stimulation. Calpain inhibitors rescued degradation of full-length ARMS/Kidins220 as well as the accumulation of degradation products (Fig. 6A and B). Calpain inhibitors also rescued the ARMS/Kidins220 degradation induced by glutamate treatment (Fig.  6C).
To demonstrate that the degradation of ARMS/Kidins220 is not due to non-specific proteolysis of membrane proteins, we probed for AMPA receptor subunits GluA1 and GluA2, two synaptic membrane proteins. After KCl depolarization, GluA1 was degraded in a calpaindependent manner, consistent with previous studies (Fig. 6D)(9,16,29). However, GluA2 remained intact, indicating that the activity of calpain was upon specific targets.
Taken together, these data indicate that ARMS/Kidins220 is a specific target of calpain, which degrades the scaffold molecule in an activity-dependent manner.
Inhibition of calpain activity rescues the LTP enhancement in ARMS/Kidins220 +/mice. A possible explanation for the enhancement of LTP in ARMS/Kidins220 +/mice is that a further reduction of the protein levels in these animals by calpain cleavage unmasks a role for ARMS/Kidins220 in regulating the amounts of synaptic potentiation. To demonstrate a link between the enhancement of LTP observed in ARMS/Kidins220 +/mice and calpain-dependent ARMS/Kidins220 proteolysis, we studied the effect of calpain inhibition in the mice. Acute hippocampal slices were taken from 3-to 6month-old ARMS/Kidins220 +/mice, and LTP was induced in the presence and absence of BDA-410, a compound that has been shown to be a potent and selective calpain inhibitor (30,31). In a previous study, calpain inhibition by BDA-410 had no effect on the expression of LTP or on basal transmission in wild-type mice (32). Our experiments confirmed that this inhibitor also did not affect LTP in our wild-type animals ( Fig. 7; wild-type mice treated with vehicle [black circles] vs. BDA-410 [black triangles] -F(1,13) = 0.14, p > 0.05, two-way ANOVA). However, the inhibitor did rescue the enhancement of LTP in ARMS/Kidins220 +/mice ( Fig.  7; ARMS/Kidins220 +/mice treated with vehicle [white circles] vs. BDA-410 [white triangles] -F(1,12) = 4.907, p < 0.05, two-way ANOVA). These data indicate that the effects of ARMS/Kidins220 expression on LTP can be mediated by calpain.

DISCUSSION
Activity-dependent regulation of synaptic proteins is a critical process responsible for many observations of synaptic plasticity. Here we demonstrated that decreased levels of the ARMS/Kidins220 scaffold protein in vivo led to an enhancement of LTP. In cultured neurons, ARMS/Kidins220 was downregulated in an activity-dependent manner, and the decrease in ARMS/Kidins220 levels was regulated by calpain. Inhibition of calpain activity in ARMS/Kidins220 +/mice rescued the changes in LTP. Taken together, our data suggests that the activity-dependent regulation of ARMS/Kidins220 by calpain mediates its effects on synaptic plasticity.
It has been proposed that regulated physiological activation of calpain is critical for synaptic plasticity and memory formation, while the pathological hyperactivation of calpain leads to neurodegenerative processes (3,4). Normal activation leads to key signaling processes, while abnormal activation leads to dysregulated degradation and neurotoxicity. Lopez-Menendez and colleagues have shown that ARMS/Kidins220 plays a key role during NMDA-mediated excitotoxicity (33). Here, we have found that calpain cleavage of ARMS/Kidins220 is involved in its ability to influence LTP. We previously showed that alterations in ARMS/Kidins220 levels in culture led to changes in synaptic charge, suggesting that ARMS/Kidins220 regulates synaptic activity (21). Recent studies in hippocampal neurons indicate the level of ARMS/Kidins220 protein expression can influence both excitatory (34) and inhibitory neurotransmission (35). Intriguingly, lowered ARMS/Kidins220 protein levels in vivo caused an enhancement of LTP.
We used the highly selective BDA-410 calpain inhibitor to assess whether calpain activity is linked to the changes in LTP observed in heterozygous mice expressing reduced levels of ARMS/Kidins220. Consistent with previous findings, the BDA-410 inhibitor did not have any effect upon the generation of LTP or of basal synaptic transmission properties in wild-type mice (32). However, treatment of hippocampal slices from ARMS/Kidins220 +/mice resulted in a reversal of the enhancement of LTP.
These results imply that calpain activity is related specifically to the generation of LTP in mice with a reduced level of ARMS/Kidins220. Steady state levels of ARMS/Kidins220 in wildtype animals may place a constitutive restraint upon potentiation, perhaps through the inhibition of a rate-limiting step. Decreases in the ARMS/Kidins220 protein in ARMS/Kidins220 +/mice would prime or facilitate the ability to generate LTP at Schaffer collateral-CA1 synapses. The specific action of the BDA-410 calpain inhibitor upon the ARMS/Kidins220 +/mice suggests that calpain cleavage of ARMS/Kidins220 or its many associated proteins may be responsible for changing hippocampal plasticity.
Calpain has also been shown to regulate MAPK signaling involved in memory processes through cleavage of the SCOP, an inhibitor of MAPK (36). Interestingly, BDNF was implicated in the induction of calpain to regulate SCOP. Calpain activation can also be regulated by BDNF through MAPK phosphorylation events (37). These studies provide evidence that calpain is directly involved in synaptic plasticity through the regulation of several different mechanisms. Notably, the calpain cleavage of glutamate receptor subunits is regulated by phosphorylation (29). The kinases Fyn and CaMKII, amongst others, have been shown to regulate the sensitivity of targets to calpain cleavage (38,39). As ARMS/Kidins220 is a molecule with many potential phosphorylation sites, cleavage may be regulated by the phosphorylation state of the protein.
It is likely that different physiological functions are carried out ARMS/Kidins220 since it has multifunctional interactions with many different signaling proteins, such as Trio, septins, Trk receptors, CrkL, and AMPA and NMDA receptor subunits (33,34,40-42). As a scaffold protein at postsynaptic sites, ARMS/Kidins220 can potentially participate in protein-protein interactions in dendritic spine compartments. Conspicuously, the C-terminus of ARMS/Kidins220 contains a PDZ binding motif, which has the potential to interact with PDZ domain-containing proteins in the PSD to exert its scaffolding functions. As a substrate of neurotrophin and ephrin receptors, as well as calpain, regulation of ARMS/Kidins220 levels may serve as an important convergence point for transmitting signals to mediate synaptic plasticity.

ABBREVIATIONS
The abbreviations used are: ARMS, ankyrin repeat-rich membrane spanning; CaMKII, Ca 2+ /calmodulindependent protein kinase II; GRIP, glutamate receptor interacting protein; Kidins220, kinase Dinteracting substrate of 220 kD; LTP, long-term potentiation; PSD, postsynaptic density; SCOP, suprachiasmatic nucleus circadian oscillatory protein.  Cell lysates were immunoblotted for ARMS/Kidins220, using antibodies against the C-or N-terminus, and for actin as a loading control. In response to neuronal activation, 220 kD full-length ARMS/Kidins220 as detected by both C-terminus and N-terminus antibodies is degraded, leading to the accumulation of smaller-sized fragments as detected by the N-terminus antibody (arrows), estimated to be 140 (a), 120 (b), and 85 (c) kD in size. 95 kD and 150 kD non-specific bands were detected by the N-terminus antibody. (B) Quantification of ARMS/Kidins220 protein levels in hippocampal neurons after KCl depolarization, as detected with the C-terminus antibody, normalized to actin levels, and plotted relative to untreated control (n ≥ 3 independent experiments, data are represented as the mean ± SEM). Hippocampal cultures were depolarized with 50 mM KCl for three hours in the presence of the calcium chelator EGTA, and ARMS/Kidins220 protein levels were assessed by Western blot. EGTA fully rescued the degradation of full-length ARMS/Kidins220 and the appearance of N-terminal degradation fragments (arrows a, b, and c). Actin is shown as a loading control. 95 kD and 150 kD non-specific bands were detected by the N-terminus antibody. (B) Quantification of ARMS/Kidins220 protein levels in (A), as detected with the C-terminus antibody, normalized to actin levels, and plotted relative to untreated control (n ≥ 3 independent experiments, **p < 0.01, t-test; data are represented as the mean ± SEM). Fig. 4. Glutamate activation of hippocampal neurons induces ARMS/Kidins220 degradation through a calcium-mediated process. (A) ARMS/Kidins220 is degraded upon glutamate treatment of hippocampal neurons. Hippocampal cultures were stimulated with 200 µM glutamate for one minute, and cell lysates were collected at the indicated times after stimulation and immunoblotted for ARMS/Kidins220. As is the case after KCl depolarization, full-length ARMS/Kidins220 is degraded, leading to the accumulation of similar smaller-sized N-terminal fragments (arrows a, b, and c). Actin is shown as a loading control. A 150 kD non-specific band was detected by the N-terminus antibody. (B) Glutamate-mediated ARMS/Kidins220 degradation requires extracellular calcium. Hippocampal cultures were stimulated with 200 µM glutamate for one minute in the presence of EGTA, and cell lysates were collected three hours after stimulation. ARMS/Kidins220 protein levels, as detected by the C-terminus antibody, were assessed by Western blot and quantified. ARMS/Kidins220 levels were normalized to actin levels and plotted relative to untreated control (n ≥ 3 independent experiments, **p < 0.01, t-test; data are represented as the mean ± SEM).  Both of these inhibitors significantly reduced the degradation of ARMS/Kidins220 and prevented the appearance of N-terminal degradation fragments (arrows a, b, and c). 145kD and 150 kD breakdown products of spectrin mark calpain activity, and actin is shown as a loading control. 95 kD and 150 kD non-specific bands were detected by the Nterminus antibody. (B) Quantification of ARMS/Kidins220 protein levels in (A), as detected by the Cterminus antibody, normalized to actin levels, and plotted relative to untreated control (n ≥ 3 independent experiments, **p < 0.01, t-test; data are represented as the mean ± SEM). (C) Hippocampal cultures were stimulated with 200 µM glutamate for one minute in the presence of the calpain inhibitor MDL28170 or ALLM, and cell lysates were collected at the indicated times after stimulation. ARMS/Kidins220 protein levels, as detected by the C-terminus antibody, were assessed by Western blot and quantified. ARMS/Kidins220 levels were normalized to actin levels and plotted relative to untreated control (n≥ 3 independent experiments, *p < 0.05, t-test; data are represented as the mean ± SEM). (D) Hippocampal cultures were depolarized with 50 mM KCl for indicated times in the presence or absence of the calpain inhibitor MDL28170, and the indicated proteins were probed by Western blot. The degradation of fulllength ARMS/Kidins220 as well as the appearance of N-terminal degradation fragments (arrows a, b, and c) correlated with the degradation of GluA1, and the degradation of both proteins were prevented by calpain inhibition. In contrast, GluA2 was not degraded. Fig. 7. Inhibition of calpain prevents enhancement of LTP in ARMS/Kidins220 +/mice. Acute hippocampal slices from 3-7-month-old ARMS/Kidins220 +/mice and wild-type littermates were incubated with the calpain inhibitor BDA-410 before and during LTP induction by a θ-burst stimulus (arrow). Calpain blockade did not affect LTP expression in wild-type animals while it prevented enhancement of LTP in ARMS/Kidins220 +/mice. Each point represents the average of three successive events (WT: vehicle -black circles, n = 7, BDA-410 -black triangles, n = 8; ARMS/Kidins220 +/-: vehicle -white circles, n = 7; BDA-410 -white triangles, n = 9; data are represented as the mean ± SEM).