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Rapid preparation of plasma for 'Stat' coagulation testing.

We undertook to confirm and extend the previous work on the use of microcentrifugation (2 minutes at 11,000 g) to prepare platelet-poor plasma for assay of the prothrombin time, partial thromboplastin time, fibrinogen level, D-dimer, antithrombin III, and dilute Russell viper venom time. We compared results of routinely submitted blood samples by both high-speed and routine (15 minutes at 1800g) centrifugation. We found no significant differences in assay results and concluded that the high-speed technique is a reliable and useful option for minimizing turnaround times for these coagulation assays.