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Macrophage Migration Inhibition in Experimental Diabetes

Abstract To study the cause of increased susceptibility to infections in diabetes, peritoneal macrophages from normal, untreated and insulin-treated diabetic rats were isolated using thioglycollate as an irritant. Alterations in the number of macrophages, their in vitro ability to phagocytose, and migrate from a capillary chamber in response to murine migration inhibition factor (MIF) were investigated. The macrophages from the diabetic rats exhibited decreased sensitivity to MIF and also phagocytic activity with respect to sheep red blood cells. Insulin treatment for 10 to 15 days significantly improved these characteristics toward normal.