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Architecture of Pancreatic Islets Imaged by Serial Block Face SEM

The serial block-face scanning electron microscope (SBF-SEM) comprises an automated ultramicrotome built into the specimen chamber, a high-brightness field-emission electron gun, and a high-efficiency backscattered electron detector, enabling high-resolution 3D imaging of heavyatom stained and embedded tissues [1]. The technique provides ultrastructural images of tissue volumes as large as 10 μm, with a spatial resolution of 10-nm in the plane of the block face and ~25-nm in the cutting direction. The technique complements conventional transmission electron tomography and scanning transmission electron tomography [2].