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A modification of the erythrocyte osmotic fragility test.

A method for the determination of erythrocyte osmotic fragility using the Unopette and hepannized blood collected in Vacutainer tubes is described. The prepackaging of stable buffered solutions of sodium chloride with the use of self-filling, self-measuring capillaries for collection and dilution of blood makes the osmotic erythrocyte fragility test an accurate, simple, practical procedure. The use of heparinized whole blood collected in a Vacutainer tubes obviates the difficulties inherent in the determination of the osmotic fragility of sterile defibrinated incubated blood.