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Differential gene expression at gestational days 14 and 16 in normal and nitrofen‐induced hypoplastic murine fetal lungs with coexistent diaphragmatic hernia

The purpose of this study was to identify differentially expressed genes in normal and nitrofen‐induced hypoplastic lungs in fetal mice. Such genes may play a role in the regulation of lung development. CD‐1 pregnant dams were gavaged with 25 mg of nitrofen on gestational day (Gd) 8 to induce pulmonary hypoplasia and diaphragmatic hernia (DH). Normal and nitrofen‐treated fetuses were removed on Gd 14 and Gd 16. Lungs were examined in all nitrofen‐exposed fetuses and only those that had developed severely hypoplastic lungs with coexistent diaphragmatic hernia were taken for molecular analyses. RNA was extracted from normal and nitrofen‐treated lungs, reverse transcribed, and PCR‐amplified using 48 combinations of anchor and arbitrary primers for each condition. The resulting cDNAs from normal and hypoplastic lungs were run on 6% polyacrylamide differential display gels.