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[The cloning of human augmentor of liver regeneration-interacting proteins].

AIM
To screen human augmentor of liver regeneration(hALR)-interacting proteins and to explore the mechanisms of hALR in liver regeneration.


METHODS
The open reading frame of hALR was used to construct the "bait" plasmid and the genes encoding hALR-interacting proteins were screened from the human liver cDNA library that was pretransformed into yeast Y187 by yeast two-hybrid system. Bioinformatic analysis of the sequences of the positive clones were performed.


RESULTS
The positive clones encoding metallothionein, albumin, selenoprotein P,Na/K-ATPase, and 2 unknown proteins were screened out.


CONCLUSION
The successful cloning of the genes encoding proteins interacting with hALR may pave the way for studying the interaction between the above proteins and hALR, and the molecular mechanisms of biological effect of hALR.