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199 An Enzyme-based Protein Digestibility Assay

The Immobilized Digestive Enzyme Assay (IDEA) measures the αamino groups liberated during protein peptide bond hydrolysis. This paper presents the IDEA system designed for feed ingredients and the comparison and correlation of the determined IDEAs with both ingredient processing and in vivo rooster assays. Correlation (r 2 )o f IDEA values with in vivo digestibilities (lysine) for soyflake and meat and bone meals was 0.91 and 0.92, respectively. IDEA values correlated well with the in vivo digestibilities of soyflake processing and demonstrated that overprocessing can be detected using this technique. The IDEA assay is easy, prevents enzyme autolysis and sample contamination, and allows reuse of the enzymes (no loss of activity following 20 assays). The data suggest that the IDEA system represents a rapid, robust and inexpensive predictor of feed ingredients compared to in vivo methods.