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Amyloid &bgr;-peptide levels in laser capture microdissected cornu ammonis 1 pyramidal neurons of Alzheimer's brain

Deposition of the amyloid &bgr;-peptide (A&bgr;) is a pathophysiological event associated with Alzheimer's disease. Although much is known about the molecular composition of extracellular A&bgr; deposits, the role of the intracellular pool of A&bgr; is not fully understood. We investigated whether A&bgr; levels are increased in cornu ammonis 1 pyramidal neurons of Alzheimer's disease hippocampus, using laser capture microdissection to isolate the neurons and enzyme-linked immunosorbent assay for quantification. Our results showed increased A&bgr;42 levels and an elevated A&bgr;42/A&bgr;40 ratio in neurons from sporadic as well as from familial cases of Alzheimer's disease, whereas A&bgr;40 levels remain unchanged between the cases and controls. We speculate that intracellular accumulation of A&bgr;42 increase vulnerability of cornu ammonis 1 pyramidal neurons in Alzheimer's disease.