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Modelling the Effect of Electric Pulse on Cells and Experimental Research

The effect of electric pulse to cells is simulated with a resistor-capacitor(RC) circuit model. The transmembrane voltage equation is deduced from the RC circuit model, which is proposed according to cell model with mitochondria included. The experiment settings are designed to produce pulsed electric field with the intensity peak of 2.12 MV/m within 100 ns and 1 mus, respectively. Cell death rates are assessed after electric stimulation. With the experimental parameters and cell structural, cell electric parameters from related papers, transmembrane voltages of cytomembrane and mitochondria membrane are calculated as the functions of time. Apoptosis can be induced when the mitochondria membrane is electroporated and cell will die in another way from that induced by cytomembrane electroporation. The RC circuit model and calculation correspond with the experiment result.