{'fraction_non_alphanumeric': 0.03409090909090909, 'fraction_numerical': 0.007575757575757576, 'mean_word_length': 5.2176470588235295, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Development and standardization of a new method for Virus-Like Particle quantification by digital PCR

Introduction: The NAT PLUS HIV/HCV/HBV/MAL molecular Kit (Bio-Manguinhos) is used to complement the serological screening in Hemotherapy Services, through the detection of the nucleic acid of hemotransmitted pathogens HIV, HCV, HBV, and Malaria, aiming to increase transfusion safety. Performing an assay with high sensitivity and specificity, this kit has an internal control (IC) to validate the results of each reaction. Protected by a patent (No. PI 0600715-5), the IC is a biosecure Virus-like Particle (VLP), derived from HIV and modified by directed mutagenesis techniques to delete its ability to replicate. During the VLP production, it is essential to know the VLP concentration in each step of the process for quality control. This project has selected the digital PCR (dPCR) to quantify the VLP produced, since it has the advantage of performing absolute quantifications without standards, being mathematically accurate, and easy to operate.