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Phase correction of image plane in holographic microscopy with equal-step phase shift

A method which can compensate the phase curvature of image plane in equal-step phase-shifting holographic microscopy is presented. Recording two holograms before and after inserting the sample, unwrapping the numerical reconstructed images of the two holograms respectively and subtracting them each other, the corrected phase distribution of the sample is obtained. Firstly, the imaging characteristics of in-line phase-shifting holographic system in the image plane was analyzed. Secondly, onionskin cells and USAF1951 test target were recorded and reconstructed by this method. It is shown that high quality imaging of phase objects can be achieved by proposed method.