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High Mobility Group Protein HMO2 promotes 3′ and 5′‐ homologous strand invasion in yeast

Homologous recombination (HR) is the error‐free pathway involved in double strand break (DSB) repair. In Saccharomyces cerevisiae, the ATP‐dependent chromatin remodeling complex, INO80, has been reported to be involved in DSB repair. HMO2, a component of the INO80 complex, is a high mobility group (HMG) protein that binds to DNA in a non‐sequence specific manner. It has been shown recently that HMO2 interacts with γH2AX and helps recruiting INO80 to the region of DSB. HMO2 binds and protect DNA ends, both blunt and cohesive, and sequence of the single stranded overhang affects binding significantly. Here we show using D‐loop assay that HMO2 has the ability to mediate DNA strand invasion and annealing to produce a D‐loop‐ a crucial step in recombination repair. Further we also found that HMO2 mediates 3′ homologous strand invasion more efficiently than the 5′ strand, indicating that the polarity of the ssDNA is important for the process. This suggests that HMO2 might have pivotal implications on DSB repair in yeast beyond just INO80 recruitment. This work is supported by NSF (MCB‐0744240).