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Are TEM and CTX Beta-Lactamase genes common among urinary isolates from Ain Shams University Hospitals, Intensive Care Units?

Background: Many extended-spectrum β-lactamases (ESBLs) variants are known
among Gram-negative bacilli, and are classified into different structural families as
TEM, SHV, CTX-M, and OXA. Objectives: To detect the distribution of blaTEM and
blaCTX-M genes among the Gram-negative isolates collected from urine samples from
patients admitted to ICUs of Ain Shams University Hospitals, Egypt. Methodology:
Forty Gram-negative urinary isolates were enrolled, and subjected to microbiological
identification and antimicrobial susceptibility testing. Phenotypic detection of ESBLs
production was done. Detection of blaTEM and blaCTX-M genes was done by PCR. Results:
Phenotypically, 8(20%) isolates were ESBL producers and 32(80%) were non ESBL
producers. BlaTEM gene was found in 15 isolates(37.5%) and blaCTX-M gene was found in
8 isolates(20%), while both genes were detected among five isolates. Conclusion:
Molecular methods should be used for definitive identification of ESBLs. BlaTEM gene
was more common than blaCTX-M gene in urine specimens in our setting.