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OBM Integrative and Complementary Medicine Head Circumference as An Indicator of Nutritional Status in Critically Ill Children under 2 Years of Age

Many clinical, biochemical, and anthropometric factors have been used to determine the nutritional status of critically ill children. One of the most commonly used anthropometric measurements in children is the head circumference. It is used to assess their development, and in some instances, can be an indicator of chronic malnutrition. We aim to study the association between head circumference and the nutritional status of critically ill children under 2 years old. We retrospectively obtained the head circumference measurements of 142 patients between 30 days and 2 years of age in the pediatric intensive care unit (PICU), and calculated their head circumference percentile-for-age based prematurity, age at admission, sex, and race by multivariate analysis. We found that critically ill patients with chronic malnutrition had significantly smaller head circumference than those with a normal nutritional status. However, it would be necessary to conduct a larger study in order to confidently determine a cutoff point for head circumference percentile that can be used to detect malnutrition in the PICU.


Introduction
Given the prevalence of malnutrition in the hospital setting, it is important to identify surrogate biochemical and clinical markers in order to quickly recognize and intervene [1]. Pediatric malnutrition is defined as "an imbalance between nutrient requirement and intake, resulting in cumulative deficits of energy, protein, or micronutrients that may negatively affect growth, development, and other relevant outcomes" and chronic malnutrition is defined as present for 3 months or longer [2]. For children, commonly used indicators of malnutrition include anthropometric markers such as BMI, weight-for-length, weight-for-age, height-for-age, and birth weight [3]. Other indicators include clinical markers such as weight loss, weight gain, or food intake [4]. Physical exam findings such as muscle wasting and adipose tissue distribution are also commonly used to determine nutritional status [5]. Another anthropometric parameter that is widely used in pediatrics is the head circumference [6]. However, it is more commonly used as a marker of development, while its use as a nutritional indicator has been limited.
Neurological development is highly dependent on the person's nutrition, particularly during the first 3 years of life [7,8]. This neurological development is reflected in the development of brain volume, of which head circumference is a reliable estimate [9][10][11]. Given the relationship between nutrition and head circumference, growth charts have been developed and are used in hospitals across the country [12].
One of the main limitations of using nationally representative growth charts is that the proportion of malnutrition in the general population is smaller than that of hospitalized patients. For example, common measures of malnutrition in children under five years of age such as stunting (low height-for-age) and wasting, (low weight-for-height) correspond to 3.5% and 0.4% respectively in the United States' population [13]. In contrast, the prevalence of malnutrition in the pediatric intensive care unit (PICU) has been found to be 24-53% in countries of various levels of economic development based on different anthropometric measurements [14]. Therefore, it is important to study the relationship between head circumference measurement and chronic malnutrition in the context of PICU admission.
Given the evidence presented, we hypothesize that critically ill patients that are chronically malnourished would have a smaller head circumference than those that have a normal nutritional status. Our specific aim is to determine if nutritional status affects head circumference in critically ill children under 2 years of age. A significant relationship between head circumference and nutritional status would support the use of this anthropometric parameter in the development of a nutrition screening tool in the future.

Enrollment Criteria
These patients had been previously enrolled in a larger, currently ongoing study to develop a nutrition screening tool. This is an ancillary study within that study. We retrospectively obtained the head circumference measurement of 142 patients under 2 years of age, which included children admitted to the pediatric intensive care unit (PICU) at Children's Wisconsin between 30 days and 18 years of age. Although CDC growth curves for head circumference include ages up to 3 years, our unit does not routinely record head circumference measurements for patients over 2 years of age.
Parents or legal guardians had already provided their informed consent to participate in the parent study. We received approval to conduct this ancillary study involving human subjects by the Children's Wisconsin institutional review board (IRB).

Anthropometric Measurements
We obtained anthropometric data from the electronic health record. These anthropometric measurements were determined by skilled pediatric critical care nurses in our unit at the time of admission to the PICU in accordance with national guidelines. The measurement of head circumference consists of measuring the patient's head with a nonelastic measuring tape from the occiput to the forehead just above the supraorbital ridge. Head circumference was recorded within 24 hours of admission. Patients with head circumference missing from the admission record were excluded from the study.

Adjusting Head Circumference for Gestational Age
We obtained the head circumference measurement in centimeters and the gestational age in weeks as recorded in the electronic health record. We entered the head circumference into a web calculator using the 2000 CDC head circumference for age percentile [12]. We adjusted the head circumference percentile for gestational age at birth for any patient born before 40 weeks and then adjusted to 40 weeks' gestation.

Determination of Nutritional Status
We studied the statistical relationship between head circumference and other parameters that were obtained in the nutrition screen study. These parameters include prematurity, gestational age, demographic data, and nutritional status. The nutritional status was determined by a registered dietitian using the Subjective Global Nutritional Assessment (SGNA), which has been validated in the pediatric critical care population as a measure of nutritional status [15]. The SGNA takes into account a nutrition-focused medical history, which includes dietary intake, clinical symptoms, and weight changes, and a physical exam that measures subcutaneous fat, muscle wasting, and nutrition-related edema. The detailed methodology has been published for a precise reproduction of this assessment tool [5].

Statistical Analysis
We determined the association between head circumference and the nutritional status (based on the SGNA) using the Mann-Whitney test. We calculated the sensitivity and specificity for each head circumference percentile cut point between 0 and 100% for identification of malnutrition. Sensitivity was defined as the percentage of patients with malnutrition by SGNA who had a head circumference at or below the cut-off value, and specificity was the percentage of patients who were not malnourished by SGNA who had a head circumference above the cut-off value. Statistical analysis was performed using the R Statistical System [16] and IBM SPSS Statistics v20.

Results
A total of 142 patients were included in our study. From this cohort, 13 of them were malnourished (9.1%) and 129 had normal nutrition status (90.8%) as determined by SGNA. There was a greater representation of younger patients with more than 50% between 1 and 8 months of age (see Figure 1). However, there was no significant relationship between nutrition status and age. Other anthropometric and demographic data were compared to their nutrition status (see Tables 1  and 2). All the anthropometric parameters that we compared with nutrition status were significant, while the only demographic parameter that was significant was gestational age.   We found a significant relationship between head circumference percentile-for-age and nutrition status. The head circumference percentiles were not normally distributed and had a range from 0 to 100 percentile in patients with normal nutrition status, and a range from 0 to 65.4 percentile in patients that were malnourished (see Figure 2). We plotted a receiver operating characteristic (ROC) curve showing sensitivity and specificity for all head circumference percentiles (see Figure 3). The intersection occurs at a head circumference percentile close to 29 with a sensitivity of 76.92 and a specificity of 75.19. Additionally, we analyzed the relationship between head circumference percentile and prematurity, but the difference was not significant with a P value of 0.311. While prematurity itself was not a significant factor, the gestational age was less in malnourished patients [37 (35.3-38.0) median (IQR) in weeks] than in patients with normal nutrition status [38 (36.0-39.0) median (IQR) in weeks]. A multivariate logistic regression analysis that included prematurity, age at admission, male sex, African American and Caucasian race, and head circumference percentile showed no significant associations except for head circumference percentile, consistent with the findings from the univariate analysis.

Discussion
As we hypothesized, patients that were malnourished had a statistically significant smaller head circumference than the patients that had a normal nutritional status. It is important to notice that our head circumference measurements were adjusted for gestational age and when comparing the head circumference percentile of premature patients with term patients, no significant difference was found. From our univariate and multivariate analyses, we did not find prematurity to be a determining factor of nutritional status or head circumference percentile in our cohort. As previously noted, there was a significant difference between the gestational ages at birth in patients that were malnourished and patients with normal nutrition status, with a younger gestational age in patients that were malnourished.
Given the significant association, it is possible to use head circumference to identify chronic malnutrition in critically ill children. A screening tool that detects malnutrition allows us to prioritize these patients for early nutritional intervention. Therefore, it is preferred to have a screening tool with high sensitivity because it is ultimately better to treat falsely detected malnourished patients than delay intervention when a malnourished patient is missed. Using only our study population, an ideal cutoff point for head circumference percentile would be between 30 and 35, given that at this range, sensitivity is greater than specificity, and it is below the interquartile range for patients with normal nutritional status.
One of the major limitations of our study is the small sample size. Although we obtained statistically significant results, these might only be representative of our cohort. Other limitations are related to the retrospective nature of our study. These include the possibility of increased confounding factors, which were addressed by multivariate analysis, and the inability to establish causation, although the association that we found should be sufficient for the purpose of creating a screening tool.
Another limitation of our study is the wide variety of diagnoses, particularly chronic illnesses that are present in the pediatric intensive care unit population. Some of these are a direct cause of malnutrition, which would result in a smaller head circumference. Some other chronic illnesses, however, may result in a smaller head circumference despite normal nutrition and could contribute to a decreased accuracy in our measurements.
There are other anthropometric parameters, such as weight for height and mid-upper arm circumference, that are commonly used as part of the WHO standards [17] to assess for malnutrition. However, these tools are for children up to 5 years of age and they are focused on acute malnutrition, with very limited studies on chronic malnutrition. Furthermore, recent studies have shown that coupling these measurements together failed to recognize severe confounding factors such as edema [18]. Our study adds another data point for the development of a multifactorial screening tool that is also more specific to critically ill children under 2 years of age.
In this study we elucidated a significant difference between the head circumference of patients with chronic malnutrition and patients with normal nutrition status. With this foundation, the next step would be to validate our findings in a larger, prospective study in order to confidently determine a cutoff point that can be used to screen patients for malnutrition in the PICU. Head circumference percentile should also be used in conjunction with other significant anthropometric and clinical parameters to create a more effective nutrition screening tool.

Author Contributions
Alejandro Ochoa-Ramirez designed the study, collected data, wrote the first draft of the manuscript, and critically revised the manuscript. Evelyn M. Kuhn performed the data analysis, interpreted analysis of the data, and critically revised the manuscript. Jitsupa Sirinit collected data and critically revised the manuscript. Theresa A. Mikhailov designed the study, interpreted analysis of the data, and critically revised the manuscript. All authors reviewed and approved the final version of the manuscript.

Competing Interests
None declared.