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Comparison of lymphokine secretion and mRNA expression in the CD45RA+ and CD45RO+ subsets of human peripheral blood CD4+ and CD8+ lymphocytes

Flow cytometric analysis of human peripheral blood T lymphocytes demonstrated that the majority of the CD4+ cells were CD29+ or CD45RO+ “mature” cells while the CD8+ cells were primarily CD45RA+ “naive” cells. After an initial separation into CD4+ and CD8+ cells and a secondary separation into CD45 subsets, lymphokine secretion was assessed after phorbol 12‐myristate 13‐acetate and ionomycin or fixed anti‐CD3 stimulation. Within the respective CD45 subsets, CD4+ cells produced more interleukin (IL)‐2, IL‐4, and IL‐6; but the CD8+ cells secreted more interferon‐γ and granulocyte/macrophage‐colony‐stimulating factor. Tumor necrosis factor‐α secretion was similar in the matched CD45 subsets. Northern analysis revealed a parallel pattern of lymphokine mRNA expression in the four lymphocyte subsets. These results suggest that human CD8+ peripheral blood lymphocytes have a significant capacity to secrete lymphokines, and that the low lymphokine production observed in unseparated CD8+ cells reflects the higher percentage of less functional CD45RA+ cells.