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Intracellular assembly of newly synthesized canine cardiac myosins

To investigate how newly synthesized cardiac myosins are assembled into myofilaments, we analysed the distribution of newly produced α‐myosin heavy chain isozyme in sarcomeres by immunoelectron microscopy using a monoclonal antibody (CMA19), which is specific for α‐myosin heavy chain. Isozymic changes in myosin heavy chains from β to α type were induced in canine ventricular muscles and cultured ventricular myocytes by administration of 1‐thyroxine. We incubated the glycerinated ventricular muscles or cultured ventricular myocytes with the enzyme (horseradish peroxidase) labelled Fab fragment of CMA19. After the reaction with 3, 3′‐diaminobenzidine and osmification, we prepared ultrathin sections of the ventricular muscles or cultured ventricular myocytes and analysed their staining patterns by electron microscopy.