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The effect of commercial and pure gentamicin on the growth of human diploid lung fibroblasts.

The effect of different concentrations of pure and commercial preparations of gentamicin on the growth of human embryonic diploid lung fibroblasts was studied. The commercial preparation was slightly more stimulatory than the pure antibiotic at concentrations of 1 μg/ml and 10 μg/ml. At concentrations of 100μg/ml and 500 μg/ml the commercial preparation was toxic but the pure antibiotic still permitted growth. Cell growth was stimulated above that of the control and cell longevity in vitro increased with 10 μg/ml. Gentamicin had no effect on cell adsorption at any of the concentrations used.