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Nucleotide sequence and analysis of deletion mutants of the Escherichia coli gpt gene in plasmid pSV2 gpt.

The complete sequence of the HindIII-BamHI/BglII fragment of plasmid pSV2 gpt, which contains the E. coli gpt gene, has been determined and used to predict the amino acid sequence of the protein. The nucleotide sequence was used to create a map of eight gpt gene deletion mutants which were generated in mammalian cells.