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The Modulatory Role of MicroRNA-873 in the Progression of KRAS-Driven Cancers

KRAS is one of the most frequently mutated proto-oncogenes in pancreatic ductal adenocarcinoma (PDAC) and aberrantly activated in triple-negative breast cancer (TNBC). A profound role of microRNAs (miRNAs) in the pathogenesis of human cancer is being uncovered, including in cancer therapy. Using in silico prediction algorithms, we identified miR-873 as a potential regulator of KRAS, and we investigated its role in PDAC and TNBC. We found that reduced miR-873 expression is associated with shorter patient survival in both cancers. miR-873 expression is significantly repressed in PDAC and TNBC cell lines and inversely correlated with KRAS levels. We demonstrate that miR-873 directly bound to the 3′ UTR of KRAS mRNA and suppressed its expression. Notably, restoring miR-873 expression induced apoptosis; recapitulated the effects of KRAS inhibition on cell proliferation, colony formation, and invasion; and suppressed the activity of ERK and PI3K/AKT, while overexpression of KRAS rescued the effects mediated by miR-873. Moreover, in vivo delivery of miR-873 nanoparticles inhibited KRAS expression and tumor growth in PDAC and TNBC tumor models. In conclusion, we provide the first evidence that miR-873 acts as a tumor suppressor by targeting KRAS and that miR-873-based gene therapy may be a therapeutic strategy in PDAC and TNBC.


INTRODUCTION
The rat sarcoma (RAS) genes (KRAS, HRAS, NRAS, and MRAS) represent a family of small GTPases that are activated indirectly via external stimuli, such as ligand-dependent activation of receptor tyrosine kinases. 1,2 KRAS (Kirsten-rat sarcoma viral oncogene homolog) was the first RAS family protein identified. It primarily functions as a critical on/off switch in cell-signaling networks that connects upstream extracellular signals to downstream pathways to the nucleus. 3 Signals emanating from RAS proteins are relayed through proto-oncogene serine/threonine-protein kinase (RAF), mitogen-activated protein kinase (MAPK), and extracellular signal-regulated kinase (ERK)1/2 (also known as MAPK1/3) to the nucleus, where downstream transcription factors, including ETS-1, ETS-2, ELK-1, FOS, and MYC, drive transcriptional programs of cell proliferation and survival, including migration, invasion, cytoskeletal changes, and the cell cycle. [4][5][6] Activating KRAS gene mutations occur in one-third of human cancers, including adenocarcinomas of the pancreas (80%-90%), colon (45%), and lung (30%-50%), 7 and also in biliary tract malignancies, endometrial cancer, cervical cancer, bladder cancer, liver cancer, myeloid leukemia, 8,9 and breast cancer. 10 Although canonical mutations in the KRAS pathway are uncommon ($5%-12%), 11,12 overexpression of KRAS 13 and transcriptional signatures of activation of the KRAS/MAPK pathway are frequently observed in breast cancer cells, 14 often accompanied by epidermal growth factor receptor (EGFR) mutations or amplifications. 15,16 Mounting evidence suggests that the KRAS/MAPK pathway is highly prevalent and constitutes a major component of oncogenic activity in triple-negative breast cancer (TNBC), more so than in other subtypes of breast cancer. 14,[17][18][19] Owing to the failure of farnesyltransferase inhibitors in clinical trials and the lack of small molecule therapeutics approved for directly targeting KRAS, 20 current strategies involve targeting downstream components in the pathway, such as mitogen-activated protein kinase kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) inhibitors. 5 Non-coding RNAs (ncRNAs) such as microRNAs (miRNAs) are small non-coding RNAs ($22 nt) that regulate gene expression at the posttranscriptional level. 21 miRNAs bind to the 3 0 UTR of their protein-target genes (mRNAs), and they suppress protein translation by either blocking the initiation of translation or accelerating the degradation of the target mRNAs. Since miRNAs were first discovered more than two decades ago, about 60% of all human proteincoding genes are known to be the direct targets of miRNAs. 22 Recent studies have indicated that many miRNAs are aberrantly expressed in tumor cells and contribute to tumorigenesis and tumor progression by regulating signaling pathways, apoptosis, angiogenesis, the cell cycle, senescence, migration, and metastasis. [23][24][25] In this study, we found that expression of miR-873 was reduced in pancreatic ductal adenocarcinoma (PDAC) and TNBC cells and associated with significantly longer patient survival, indicating a tumor suppressor function in pancreatic and breast cancer patients. At the (F) KRAS protein expression is activated in basal-like triple-negative breast cancer cells compared to luminal types. Reduced expression of miR-873 is associated with poor overall survival in patients with PDAC (G; n = 177, p = 0.0293) and BLBC (H; n = 118, p = 0.0008), as determined by Kaplan-Meier analysis. The number of patients at risk in the low and high miR-873 groups at different time points is presented inside the graph. (I and J) Expression levels of miR-873 in PDAC (I) and TNBC (J) cell lines and normal pancreatic ductal epithelium and normal breast epithelial MCF10A cells were determined by qRT-PCR. Data were normalized to the expression of GAPDH and represent means + SE of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. molecular level, our findings elucidate the role of miR-873 in targeting KRAS, which critically controls PDAC and TNBC progression. Meanwhile, miR-873 inversely correlates with KRAS expression, which also is associated with shorter patient survival. Restoration of miR-873 expression of PDAC and TNBC models suppressed cell proliferation, migration, invasion, and tumorigenesis by inhibiting the KRAS/ERK and KRAS/PI3K axes. Overall, in vivo therapeutic delivery of miR-873 could be a potential novel therapeutic strategy to control KRAS signaling in PDAC and TNBC.

Increased KRAS Levels Are Associated with Poor Clinical Outcomes in Patients with PDAC and TNBC
To explore the clinical significance of KRAS expression, we analyzed a subset of patients with PDAC and basal-like breast cancer (BLBC) from The Cancer Genome Atlas (TCGA) by the Kaplan-Meier method, and also, we used the PROGgeneV2 tool 26 incorporating survival data associated with KRAS in patients with TNBC. Patients with high KRAS expression had significantly lower overall survival rates than did patients with low expression (PDAC: n = 177, p = 0.0064; BLBC: n = 172, p = 0.1956; Figures 1A and 1B; TNBC: n = 60, p = 0.0045; Figure S1A). To identify the differentially expressed KRAS mRNA in pancreatic tissues, we found that KRAS mRNA was significantly upregulated in human pancreatic tumor tissues (n = 45) compared to normal tissues (n = 45) (p = 3.26eÀ06) according to the GEO database (GEO: GSE28735) ( Figure S1B). At the same time, its expression was markedly high in BLBC tissues (n = 14) with respect to normal breast tissues (n = 14) (p = 0.00012), according to TCGA database ( Figure S1C). qRT-PCR analysis showed that the KRAS gene was significantly upregulated in most PDAC and TNBC cells (p < 0.05; Figures 1C and 1D). Meanwhile, basal expression of KRAS mRNA was checked in a pancreatic and breast cancer cell panel according to the data from cancer cell line Encyclopedia (CCLE) (Figures S1D and S1E). The median survival for each set is included in Table S1. On the protein level, we noticed marked upregulation of KRAS protein expression level in most of pancreatic cancer cells in comparison to normal pancreatic cells ( Figure 1E). Meanwhile, malignant basaltype (MDA-MB231, MDA-MB-436, and BT20) breast cancer cells had a higher expression level of KRAS compared with luminal-type breast cancer cell lines (MCF7, SKBR3, and T47D; Figure 1F).

Reduced miR-873 Expression Is Associated with Poor Overall Survival in Patients with PDAC and TNBC
To identify clinically relevant miRNAs that bind and regulate the KRAS gene, we first used computational algorithms, including TargetScan, 27 Diana tools, 28 microRNA.org, 29 and miRWalk2.0, 30 which predict the potential of miRNAs targeting the 3 0 UTR of KRAS mRNA. Among the potential miRNAs, miR-873-5p was identified owing to its significance in overall survival in patients with PDAC and TNBC ( Figure S2A). To elucidate the clinical significance of miR-873 expression, we analyzed a subset of patients with PDAC and BLBC from Kaplan-Meier data, and also, we used the miRpower tool (http://kmplot.com/analysis/) to analyze survival data associated with miR-873 in patients with TNBC. The overall survival rate was dramatically higher in patients with high miR-873 expression than in patients with low miR-873 expression (PDAC: n = 177, p = 0.0293; Figure 1G; BLBC: n = 118, p = 0.0008; Figure 1H; TNBC: n = 182, p = 0.037; Figure S1F). The median survival for each set is provided in Table S1.   Figure S3A). On the other hand, the blocking of miR-873 with antimiR had a reverse effect on the protein expression of KRAS ( Figure 2I).
Next, we examined the direct role of miR-873 in posttranscriptional regulation of KRAS gene expression. Using online prediction tools, we identified the consensus sequences on the 3 0 UTR of KRAS mRNA ( Figure 2J), and we performed luciferase reporter assays to show that miR-873 directly binds to KRAS mRNA in its 3 0 UTR. The binding site for miR-873 in the KRAS 3 0 UTR was cloned into a pEZX-MT06 miRNA reporter vector that also contains the luciferase gene (pEZX-MT06-3 0 UTR). The resulting plasmids were cotransfected into HEK293, PANC1, and MDA-MB-231 cells along with the miR-873 or the miRNA control. Luciferase activity was measured 48 h after transfection. We found that the luciferase expression was significantly decreased in cells that were co-transfected with miR-873 and the KRAS 3 0 UTR compared with the controls ( Figures  2K-2M). However, no decrease was observed in cells that were cotransfected with miR-873 and the mutant KRAS 3 0 UTR compared with the controls (Figures 2K-2M).

miR-873 Suppresses Proliferation and Colony Formation of PDAC and TNBC Cells
To examine the short-term effects of miR-873 on cell growth, we per-   We further examined the effects of miR-873 on PDAC and TNBC clonogenicity using a colony formation assay. miR-873 expression significantly decreased colony formation in PANC1, MiaPaCa-2, MDA-MB-231, and MDA-MB-436 cells (p < 0.05) compared with untreated or control miRNA-transfected cells ( Figures 3E-3H).

Ectopic Expression of miR-873 Induces Cell Apoptosis by Regulating Caspase-Dependent Apoptosis Pathways
Inhibition of cell growth in cancer cells is usually associated with concomitant activation of cell death pathways. We, therefore, examined the contribution of apoptosis to growth inhibition mediated by miR-873 overexpression. We evaluated the rate of cellular apoptosis using Annexin V and propidium iodide ( The induction of apoptosis was further confirmed in PDAC and TNBC cells by the expression of apoptosis-related proteins, including caspase-3, poly ADP ribose polymerase (PARP), and caspase-7, on western blot ( Figure 3K).

miR-873 Suppresses Migration and Invasion in PDAC and TNBC Cells
To investigate the effect of miR-873 on migration, we performed the scratch wound-healing assay. As shown in Figures  To elucidate the role of miR-873 expression in regulating PDAC and TNBC cell invasion, we performed the in vitro matrigel invasion assay after transfection with miR-873 mimic and control miRNA. Ectopic overexpression of miR-873 significantly reduced the number of invading PANC1, MiaPaCa-2, MDA-MB-231, and MDA-MB-436 cells by 78.42%, 85.72%, 83.62%, and 83.08%, respectively, in comparison with miR-control ( Figures 4E-4H). Together, these results suggest that miR-873 expression suppresses invasion in both PDAC and TNBC cells.

miR-873 Inhibits Pathways Downstream of KRAS
KRAS signaling leads to the induction of clinically significant downstream pathways, including protein kinase B (AKT) and ERK, which are currently being targeted by small molecularly targeted therapeutics owing to the lack of approved KRAS inhibitors. 31,32 To determine whether miR-873-induced inhibition of KRAS signaling reduces these signaling pathways in PDAC and TNBC cells, we expressed miR-873 in the related cell lines, and we examined the activity of these pathways. Expression of miR-873 in PANC1, MiaPaCa-2, MDA-MB-231, and MDA-MB-436 cells markedly decreased the phosphorylation levels of p-AKT (Ser473) and p-ERK (Thr202/Thy204) ( Figure 4I), p-C-RAF (Ser338) , C-RAF, p-MEK1/2 (Ser217/221) , and MEK ( Figures S6A and S6B). To test that our miRNA was delivered effectively to tumor tissues, we did qRT-PCR in PANC1 tumor tissues. Mice treated with miR-873 showed a marked increase in miR-873 expression level compared to those treated with miR-control ( Figure S9). Tumor tissues were also and PI double staining, and positive cells were detected and quantified by fluorescence-activated cell sorting (FACS) analysis. The represented percentages show positive cells at both early and late apoptosis. Data are represented as mean ± SE. **p < 0.01 indicates a significant difference compared with the control group. All experiments were independently performed three times. (K) Apoptotic-related protein expression was detected by western blot. miR-873-induced apoptosis was manifested after 72 h of transfection by an increase in cleavage of caspase-3, caspase-7, and PARP. b-actin was used as a loading control.   analyzed by western blot for the effects of miR-873 delivery on KRAS expression. Tumors from miR-873-treated mice showed reduced expression of KRAS compared with tumors from mice treated with control miRNA (Figures 6C, 6D, 7C, and 7D). Furthermore, there were no significant changes in mouse body weights at the end of the treatment period ( Figures S10A and S10B), suggesting that miR-873-based delivery exerted no observed side effects.
We used immunohistochemistry staining for Ki-67 in isolated tumor tissues to examine the biological effects of miR-873 on tumor cell proliferation. 33  Furthermore, our reverse-phase protein array (RPPA) analysis revealed that miR-873 transfection caused downregulation for many proteins related to the RAS/MAPK pathway, such as P-ERK, S6_pS240-pS244, and S6_pS235-pS236. By using ingenuity pathway analysis according to the RPPA results, miR-873 treatment led to significant changes in proteins and canonical pathways related to cancer signaling, such as cell cycle, angiogenesis, and colony formation (Figures 8A-8C; Figures S11A and S11B).

DISCUSSION
The findings presented here suggest that miR-873 is a clinically significant tumor suppressor miRNA whose reduced expression is associated with poor clinical prognosis and survival in patients with PDAC and TNBC. Restoration of miR-873 in PDAC and TNBC cells directly and significantly suppresses KRAS and its downstream ERK-MAPK-and PI3K-signaling pathways, which is one of the major drivers of PDAC and TNBC cell proliferation, invasion, and tumor growth. Our study also provides the first evidence that therapeutic replacement of miR-873 using systemically injected nanodelivery inhibits tumor growth in four different PDAC and TNBC models.
Activating mutations of KRAS are detected in more than 90% of patients with PDAC, and overexpression of KRAS and activation of the RAS/MAPK pathway are frequently observed in TNBC; 13,14 these major oncogenic activities represent potential molecular targets in these cancers. [17][18][19] Although molecular findings provide a strong rationale for developing novel therapeutic treatments targeting KRAS, direct inhibition of KRAS has proven to be very challenging, 20 leading KRAS to be classified as an undruggable target. 34 Therefore, there is a crucial demand to discover more effective treatments for pancreatic and breast cancers that harbor KRAS mutations.
In the current study, we found that miR-873 has a very low expression in PDAC and TNBC cells compared with normal cells, suggesting that miR-873 is frequently dysregulated in pancreatic and breast tumors. To date, several studies have described the role of miR-873 in different types of cancers, including breast cancer, 35 glioma, 36 lung adenocarcinoma, 37 ovarian cancer, 38 and colorectal cancer. 39 miR-873 was reported to inhibit the expression of genes such as CDK3, 35 IGF2BP1, 40 Bcl-2, 36 and TRAF5 and TAB1. 39 However, the biological roles of miR-873 and its direct functional targets in PDAC and TNBC have not yet been elucidated. A key finding in our study was the regulation of KRAS by miR-873, which was shown to exert a tumor-suppressive effect in PDAC and TNBC, both in vitro and in vivo.
Oncogenic KRAS promotes pancreatic tumorigenesis through the activation of multiple downstream pathways, including RAF/MEK/ ERK, MAPK, PI3K/Akt, Bad, and nuclear factor kB (NF-kB). 41,42 Inhibition of KRAS and its downstream pathways has been shown to reduce tumor growth and enhance gemcitabine chemotherapeutic efficacy against pancreatic cancer. 43,44 Downstream effectors of KRAS signaling in pancreatic cancer have been widely explored. Among them, Ras/Raf/ERK and Ras/PI3K/AKT signals are major effector pathways in PDAC tumorigenesis, 44 especially through cell cycle regulation. 45 Recent observation showed that ERK could function with AKT synergistically to promote PDAC tumorigenesis. 46 Furthermore, the expression of miR-873 in PDAC and TNBC cells inhibited the AKT and ERK pathways by directly targeting KRAS and inhibiting its translation. Thus, key effectors in KRAS signaling may be tightly regulated by miR-873 in the pancreas, and miR-873 restoration could effectively target Ras/Raf/ERK and Ras/PI3K/AKT to suppress PDAC and TNBC growth.
As our multiple in vivo studies suggested, targeting of KRAS by miR-873 is expected to be a highly efficient therapeutic strategy in PDAC, TNBC, and other KRAS-driven cancers. In breast cancer, KRAS/MAPK signaling plays an important role in growth signaling from the extracellular environment. 47,48 Activation of the KRAS/ MAPK-signaling pathway induces numerous responses in cancer ***p < 0.001, ****p < 0.0001. (C-H) Nanoparticle delivery of miR-873 inhibits KRAS expression (C and D) and intratumoral proliferation (E and F) and induces apoptosis (G and H) in PDAC orthotopic xenograft mouse models. Immunohistochemical staining was used to evaluate the expression of proliferation marker Ki-67 and in vivo apoptosis marker TUNEL in PANC1 and MiaPaCa-2 mouse xenografts treated with nanoparticle miR-873 or control miRNA mimic (E and G). Positively stained cells in both treatment groups were quantified (F and H). Data represent means + SE. *p < 0.05, **p < 0.01, ***p < 0.001. www.moleculartherapy.org cells, regulating cell proliferation, differentiation, migration, and invasion. In addition, noncanonical activation of KRAS/MAPK signaling has been shown to be regulated in breast cancer tissues by increased expression levels of EGFR, HER2, and insulin-like growth factor receptor. [49][50][51] Furthermore, germline polymorphism in the KRAS 3 0 UTR is considered a genetic marker of increased risk of TNBC progression in premenopausal women. 52 Previous studies showed that KRAS was regulated by several miR-NAs, including let-7, miR-16, miR-143, and miR-1298 in breast cancer cells; miR-4689 in colon cancer; and miR-193b in PDAC cells. [53][54][55][56] In the current study, we demonstrated that miR-873 is a clinically significant miRNA and reduced expression of miR-873 is associated with significantly reduced overall survival, whereas restoration of miR-873 expression decreases cell proliferation, migration and invasion, and tumorigenesis by downregulating KRAS expression levels in PDAC and TNBC tumor models. Meanwhile, these effects were rescued by the overexpression of KRAS. Therefore, miR-873 may be one of the most important miRNA regulators of the KRAS oncogene, and these findings have the potential for translation into clinical studies for therapeutic applications. Interestingly, miR-873 was found to significantly induce apoptosis in PDAC and TNBC cells. This observation was confirmed by different methods (western blot detection of the apoptosis markers PARP, caspase-3, and caspase-7 and flow cytometry analysis of the Annexin V staining). This result is in parallel with that of the previous finding of Chen et al., 36 who showed that this miRNA per se induced apoptosis in glioma. Most importantly, the inhibition of tumor growth in an orthotopic xenograft mouse model of PDAC and TNBC via lipid-based nanoparticle delivery of miR-873 suggests that strategies targeting the miR-873/ KRAS axis may provide broad antitumor effects through the inhibition of multiple oncogenic pathways.
In summary, our findings provide new insight into the posttranscriptional regulation of mutant and wild-type KRAS by miR-873 in PDAC and TNBC tumorigenesis. In particular, we showed that miR-873 regulates important hallmarks of cancer, including cell proliferation, apoptosis, and invasion, through the targeting of multiple oncogenic routes involving KRAS-induced ERK/AKT signaling. Hence, these findings may have important translational implications; miR-873-based therapy might be an attractive therapeutic strategy to treat PDAC and TNBC.

Survival Analyses
To investigate the relationship between KRAS or miR-873 expression and overall survival, clinical data associated with 172 basal-like breast tumors 57,58 and 177 pancreatic tumors 59 were obtained from TCGA cohorts. Patients were divided into KRAS or miR-873 high or low groups based on median KRAS or miR-873 expression for each tumor type. For each analysis, differences in overall survival (OS) were calculated by a log-rank test, and the hazard ratio (HR) is reported.
To investigate the differences in KRAS mRNA expression in tumor versus normal tissue, gene expression data from 14 basal-like breast cancer samples and 45 pancreatic samples with matched tumor and adjacent normal tissue were obtained from TCGA and GEO: GSE28735, respectively. 60 Differences in KRAS expression for basal-like breast cancer and pancreatic cancer were calculated using a Student's t test, with p < 0.05 considered statistically significant Capan-2 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) and a 100-U/mL penicillin-streptomycin solution (Sigma). All media were supplemented with 10% FBS and a 100-U/mL penicillin-streptomycin solution. MCF-10A cells were maintained in a nutrient mixture consisting of DMEM/ F12 supplemented with 5% horse serum, epidermal growth factor, hydrocortisone, insulin, and cholera toxin. All cultured cells were incubated at 37 C in a water-saturated 95% air-5% CO 2 atmosphere.

Cell Viability and Colony Formation Assays
The viability of TNBC and PDAC cells was analyzed using the MTS assay as previously described. 61 A total of 1 Â 10 3 to 2 Â 10 3 cells/well were seeded in 96-well plates. Cells were incubated overnight and then treated with a synthetic RNA oligonucleotide. Cell viability was determined at 24, 48, 72, and 96 h using 5 mg/mL MTS. We analyzed the plates at a wavelength of 490 nm in a VMax kinetic ***p < 0.001, ****p < 0.0001. (C-H) Nanoparticle delivery of miR-873 inhibits KRAS expression (C and D) and intratumoral proliferation (E and F) and induces apoptosis (G and H) in TNBC orthotopic xenograft mouse models. Immunohistochemical staining was used to evaluate the expression of proliferation marker Ki-67 and in vivo apoptosis marker TUNEL in MDA-MB-231 and MDA-MB-436 mouse xenografts treated with nanoparticle miR-873 or control miRNA mimic (E and G). Positively stained cells in both treatment groups were quantified (F and H). Data represent means + SE. *p < 0.05, **p < 0.01.
ELISA microplate reader (Molecular Devices, Sunnyvale, CA). For the colony formation assay, cells were seeded in 12-well plates at a low density (500 cells/plate), transfected with miRNA or siRNA and the respective negative controls, and allowed to grow until visible colonies appeared. The colonies were then stained with crystal violet and counted. Each experiment was performed in triplicate.

RNA Isolation and Real-Time qPCR
MiRNeasy Mini Kit (QIAGEN) was used to isolate total RNA according to the manufacturer's guidelines, and a total of 1,000 ng RNA was used as a template and then reverse transcribed to cDNA using the qScript miRNA cDNA Synthesis Kit (Quanta BioSciences, Beverly, MA). The expression level of miR-873 was measured with the PerfeCTa miRNA Assay Kit (Quanta BioSciences) using miRNA primers from Quanta BioSciences, using real-time qPCR and normalized to the level of U6 small nuclear RNA (RNU6; Quanta BioSciences), which was used as an endogenous control.
To quantify KRAS mRNA, we synthesized cDNA from the isolated RNA using a QuantiTect reverse transcription kit (QIAGEN). qRT-PCR was carried out with BX-384 Bio-Rad using the QuantiTect SYBR Green PCR kit (QIAGEN), according to the manufacturer's protocol. All reactions were performed in triplicate. KRAS mRNA expression levels were normalized to the internal control, glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The sequences of the sense and anti-sense KRAS primers were 5 0 -ATTGT-GAATGTTGGTGT-3 0 and 5 0 -GAAGGTCTCAACTGAAATT-3 0 , respectively. The sequences of the sense and anti-sense GAPDH primers were 5 0 -CAAGGTCATCCATGA CAACTTTG-3 0 and 5 0 -GTCCACCACCCTGTTGCTGTAG-3 0 , respectively. Relative quantification of mRNA and miRNA expression was calculated using the comparative threshold cycle (2 ÀDDCt ) method.
Luciferase Reporter Assay for miR-873 Target Gene Binding and Expression PANC1, MDA-MB-231, and HEK293 normal epithelial cells were transfected with pEZX-MT06 miRNA reporter vectors containing the binding sites for miR-873 in the 3 0 UTR of KRAS and the luciferase gene (GeneCopoeia, Rockville, MD). Cells were plated (5 Â 10 4 cells per well) in each well of a 24-well plate 24 h before transfection. The cells were transfected with the pEZX-MT06 vector (200 ng) and 50 nM miR-873 mimic or control miRNA. Luciferase activity was measured 48 h after transfection using the Luc-Pair miR Luciferase Assay (GeneCopoeia). For each sample, firefly luciferase activity was normalized to Renilla luciferase activity.

RPPA
RPPA analysis was performed at the Functional Proteomics RPPA Core Facility of The University of Texas MD Anderson Cancer Center. The RPPA assay was performed as previously described. 61,63 Apoptosis Cell apoptosis was assessed by Annexin V/PI staining using a fluorescein isothiocyanate (FITC) apoptosis detection kit (BD Biosciences), according to the manufacturer's protocol. Apoptotic cells were analyzed with a FACSCalibur flow cytometer (BD Biosciences). We used CellQuest Pro software (BD Biosciences) to detect the number of apoptotic cells. Apoptosis was also assessed by detection of the cleavage of caspase-3, caspase-7, and PARP via western blotting.

Wound-Healing Assay
To measure cell motility and migration, we used an in vitro woundhealing assay. PANC1, MiaPaCa-2, and BxPC-3 cells (4 Â 10 5 cells/ well), as well as MDA-MB-231 and MDA-MB-436 cells (2 Â 10 5 cells/well), were plated in six-well plates and cultured in medium containing 10% FBS. After 24 h of incubation, cells were transfected with control miRNA or miR-873 mimic, KRAS siRNA, or control siRNA. The second day after transfection, we used a 200-mL sterile pipette tip to do straight scratch on the confluent cell layers. Then cells were photographed using a phase-contrast microscope (Nikon Eclipse TE-200-U) at 0 h. The cells were continued in incubation, and images were taken again at 24 and 36 h. The wound healing was displayed by comparing photographs taken at 0 h with those taken 24 and 36 h later. At least 5 random non-overlapping pictures for each experiment were tested and quantitated using ImageJ software (NIH, Bethesda, MD).

Matrigel Invasion Assay
Transwell inserts (4 mm pore size; Fisher Scientific) coated with a Matrigel basement membrane (0.7 mg/mL; BD Biosciences) were used for this assay. After 48-h transfection with 100 nM miR-873, control miRNA, KRAS siRNA, or control siRNA, 8 Â 10 4 (PANC1, MDA-MB-231, and MDA-MB-436) and 100 Â 10 4 (MiaPaCa-2) cells were resuspended in 500 mL serum-free medium and then added to the upper chamber of the insert. Lower chambers were supplied with 500 mL medium supplemented with 10% FBS. After 24 h of incubation, non-invading cells on the upper chamber of the filter were cleared with cotton swabs. Cells that invaded through the Matrigel onto the lower chamber were fixed, stained with the Hema-3 Stain System (Fisher Scientific), and photographed. All experiments were performed in triplicate and invaded cells to the lower side of the filter were counted in at least 5 fields and expressed as a percentage of invasion.

Tumor Xenograft Models
All studies were conducted according to an experimental protocol approved by the MD Anderson Institutional Animal Care and Use Committee. To generate orthotopic TNBC tumors, we obtained athymic female nude mice (4-5 weeks old) from the Department of Experimental Radiation Oncology at MD Anderson, and we orthotopically injected TNBC cells (MDA-MB-231, 2 Â 10 6 in 20% matrigel; or MDA-MB-436, 3 Â 10 6 in 20% matrigel) into the mammary fat pad of each mouse. For PDAC tumor models, we established tumors by injecting PANC1 (5 Â 10 6 in 30% matrigel) or MiaPaCa-2 cells (2 Â 10 6 in 20% matrigel) into the right flank of each mouse. At 14 days after injection, when the size of the tumor reached about 3-5 mm, we started liposomal-miRNA treatment as previously described. 64 Each mouse was injected with miR-873 or control miRNA (0.3 mg/kg equivalent of 8 mg/mouse once a week) in a volume of 100 mL for 5 weeks (total of five intravenous [i.v.] injections) through the tail vein. Tumor sizes were measured regularly with an electronic caliper. When treatment was completed, mice were euthanized with CO 2 and weighed to measure tumor growth, and tumor tissues were reserved for further analysis.

Immunohistochemical Analysis
Immunostaining for Ki-67 was processed to estimate cell proliferation, in formalin-fixed paraffin-embedded tumor tissues, according to the manufacturer's protocol. Briefly, slides were deparaffinized by heating at 55 C for 30 min, then exposed to xylene, and rehydrated via a series of descending series of ethyl alcohol solution. After that, we incubated slides in antigen retrieval solution (Dako) at 95 C for 30 min. Endogenous peroxidases activity was blocked by putting slides in methanol containing 3% hydrogen peroxide for 15 min. The slides were incubated with Ki-67 primary antibody (Abcam, Cambridge, MA) at 4 C overnight. Then, secondary antibodies were applied to the sections at room temperature for 1 h. Afterward, the sections were counterstained with hematoxylin for 30 s and analyzed by microscopy (Nikon Eclipse TE-200-U).

TUNEL Assay
The TUNEL assay (Promega, Madison, WI) was used to detect and quantify in vivo apoptotic cells in tumor tissues by measuring nuclear DNA fragmentation, according to the manufacturer's recommended protocol. The assay was performed as previously described. 61

Statistical Analysis
Unless otherwise specified, data were expressed as the mean ± SE of three independent experiments. The Student's t test was to determine statistical significance (set at p < 0.05). Student's t tests and ANOVA were calculated using GraphPad software.

CONFLICTS OF INTEREST
The authors declare no competing interests.
Mission Sector, Joint-Supervision Program, Egypt. The Functional Proteomics RPPA Core Facility at MD Anderson was used as part of this research.