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New immunohistochemical markers in testicular tumors.

The annual incidence of testicular neoplasms has doubled in the last 40 years, with an estimated 7,500 new cases of germ cell tumor each year. The role of immunostaining has increased with the introduction of several novel markers in the last decade. The role of the following markers in differential diagnosis is featured: alpha-fetoprotein, c-kit, CD30, cytokeratin AE1/3, glypican-3, human chorionic gonadotropin, OCT3/4, NANOG, p63, placental-like alkaline phosphatase, topoisomerase II, and VASA.