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A Simultaneous Analytical Method for Catecholamines, Indoleamines, and Related Compounds in 11 Rat Brain Regions

Abstract: A simple high‐performance liquid chromatographic method that allowsthe determination of 10 biogenic amines and related compounds for crude brain extracts has been developed. The compounds that can be quantified comprise norepinephrine, epinephrine, 3,4‐dihydroxyphenylacetic acid, 3‐methoxy‐4‐hydroxyphenylglycol, dopamine, 5‐hydroxyindole‐3‐acetic acid, homovanillic acid, 3‐methoxytyramine, 5‐hydroxytryptamine and tryptophan. The detection system involves a three electrode‐coulometric determination followed by fluorometric determination. This method is highly selective and sensitive, and in the present study, the usefulness of this methodology was confirmed by applying it to the determination of the levels of these various substances in 11 rat brain regions.