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Effects of ACTH and an inhibitor of 3 beta-hydroxysteroid dehydrogenase on the synthesis of adrenocortical mitochondrial proteins.

In experiments reported recently (J Clin Endocr 29: 539, 1969) there were changes in the synthesis of guinea pig adrenal micro-somal proteins after in vivo exposure to an inhibitor of 3β-hydroxysteroid dehydrogenase, 2α-cyano-4,4,17α-trimethylandrost-5-en-17β-ol-3-one (cyano-ketone), for 4 days. It was evident that ACTH, although it also stimulated the synthesis of microsomal protein, could not fully explain the effects of cyano-ketone. This report presents data on proteins of mitochondrial fractions obtained in the same experiments. The protein content of the mitochondrial fraction and protein radioactivity after 4 hr exposure to l-leucine-(3H) was increased after cyano-ketone as it had been in the microsomal fraction. Exogenous ACTH again could not explain the effect of the inhibitor on the synthesis of total mitochondrial proteins or protein fractions separated by acrylamide gel electrophoresis. Exposure to a high level of ACTH for 4 hr or 4 days seemed to exert less net effect on mitochondrial protein...