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Detection of Chemiluminescence Using an Amorphous Silicon Photodiode

A hydrogenated amorphous silicon photodiode is used for the integrated, real-time detection of chemiluminescent reactions in solution. The light emission at 425 nm, resulting from the horseradish peroxidase-catalyzed oxidation of luminol, is detected by a p-i-n amorphous silicon photodiode operated at zero bias. The sensitivity achieved is in the range of nanomole of horseradish peroxidase per liter