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Determination of oxidation stability and shelf life of cannabis
 formulations

The characterization of cannabis extract in an industrial setting is required for
 both quality assurance (QA), quality control (QC) and antioxidant research and
 development (R&D) purposes. More importantly, having a rapid and straightforward
 streamlined process is essential for the daily operational success of any R&D
 department. Using physical techniques such as polarimetry, refractometry, and
 densitometry, the physical properties of three cannabis extract compositions in carrier
 oils were characterized and examined: pure Tetrahydrocannabinol (THC), Cannabidiol
 (CBD), and a THC/CBD mixture. Furthermore, a Rapidoxy 100 â€“ oxidation stability tester
 was used to investigate the shelf of our samples; these were subsequently characterized
 by polarimetry, refractometry, and densitometry. To validate and support our findings
 with the aforementioned instruments, high-performance liquid chromatography (HPLC) was
 used. To examine varying sample concentrations of THC, CBD, and THC/CBD mixtures, we
 employed polarimetry, refractometry, and densitometry. The results showed a linear
 dependence on concentration for all three instruments with good reproducibility,
 verified and supported by HPLC analysis. These rapid tests enable the identification of
 cannabis extract compositions, while additionally giving details about concentration of
 extract in the sample. Additionally, the aforementioned techniques were used for
 cannabis mixtures after and before oxidation to characterize various stages in the
 oxidative process. Oxidation of mixtures generally resulted in higher density and lower
 refractive index values, with optical rotation remaining relatively similar. These
 findings were further supported by HPLC data providing validation of these techniques.
 These simple and straightforward techniques allowed for measurements of cannabis (THC,
 CBD, and THC/CBD) rapidly and effectively. Overall, we found these simple techniques to
 be effective, rapid, and suitable for the determination and quantification of THC, CBD,
 and THC/CBD in cannabis extract for industrial applications. These strategies will pave
 the way for future R&D focused on safe antioxidant for cannabis products.