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Suppressive Effect of Bacillus amyloliquefaciens KL87 on Alternaria Blight of Ginseng

Received: September 20, 2018 Revised: November 19, 2018 Accepted: November 28, 2018 Biological control of plant disease using microorganisms such as Bacillus, Trichoderma and Streptomyces is alternative method which can reduce disease in ginseng cultivation. Antagonistic microorganisms can replace chemical agent, and reduce yield losses caused by several pathogens. The purpose of this study was to develop eco-friendly microbial agents and application technologies for the safe production of ginseng through control of ginseng aerial disease. For the selection of antagonistic microorganisms to control ginseng aerial disease, soil samples were collected from ginseng cultivation field, and 260 kinds of bacterial microbes were isolated. Among the 260 isolated microbes, KL87 strain suppressing the growth of ginseng pathogenic bacteria, Alternaria panax and Botrytis cinerea, was selected. As a result of 16S ribosomal RNA sequence analysis, KL87 strain was identified as Bacillus amyloliquefaciens. Furthermore, biochemical characteristics of KL87 strain showed high identity (99%) with Bacillus amyloliquefaciens. In the ginseng cultivation field test, Bacillus amyloliquefaciens KL87 reduced the occurrence of ginseng Alternaria blight by 75.9%. These results strongly suggested that Bacillus amyloliquefaciens KL87 could be used as microbial agents for replacement of chemical agent.