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The influence of the feeding Flour Beetle Tribolium confusum-infested fodder on the selected indices of the health status of rats.

The present experiment was designed to demonstrate differences in the degree of fodder contamination with benzoquinones at various Tribolium confusum levels, the impact of infested feed on the beetle population and the impact of infested feed on the health status of rats. The feeding studies were done on female rats divided into 3 groups: a control group and two experimental groups. Experimental groups were fed with a fodder infested by 150 individuals of T. confusum per kg (group D1) and 300 individuals of T. confusum per kg (group D2). The insects were grown in the fodder for 5 months and the contaminated fodder was given to the laboratory animals for 8 weeks. After that period the rats were sacrificed, blood was drawn for morphological, biochemical and immunological analyses, as well as the samples of internal organs were taken for histopathology. Regardless of initial degree of infestation, after 5 months incubation period the content of benzoquinones in fodder reached the maximum level that reduced beetle population. The resulting concentration to benzoquinones had no effect upon feed intake nor growth of rate, whereas caused the presence of these substances in feces, urine and also in tissues which was indicated by pathological lesions observed in the study. The results obtained point to the possibility of the benzoquinones accumulation in the organisms of farm animals fed fodder containing pests.