{'fraction_non_alphanumeric': 0.03614457831325301, 'fraction_numerical': 0.02108433734939759, 'mean_word_length': 5.389423076923077, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Quantification and Isolation of Plumbagin (Plumbago zeylanica Linn.) by High-Performance Thin-Layer Chromatography

A simple, rapid, selective, and quantitative high-performance thinlayer chromatographic (HPTLC) method has been developed for the determination of plumbagin in the extract of Plumbago zeylanica Linn. roots of different geographical sources and on marketed formulation. Densitometric analysis of plumbagin was carried out in the absorbance mode at 268 nm. The method gave spot at RF 0.58 corresponding to plumbagin. The limit of detection (30 ng) and limit of quantification (90 ng) were confirmed with the mobile phase n-butanol-chloroform-petroleum ether (0.8:1.2:4.0). Linear regression analysis data for the calibration plot for plumbagin showed good linear relationship with r2 = 0.9981 in the concentration range of 225 ng to 675 ng per spot. The method was validated for sensitivity, linearity, accuracy, precision, and specificity as per the International Conference on Harmonization (ICH) guidelines. After development and validation, the method was applied for the isolation of plumbagin from the extract. Other preliminary studies were also carried out on the raw material like determination of extractive value, ash value, loss on drying, trace metal analysis, and preliminary phytochemical screening.