Additional file 1: Table S1. of Biallelic modification of IL2RG leads to severe combined immunodeficiency in pigs

Oligos used to introduce sgRNA into px330. Each pair of forward and reverse primers were annealing and ligated into the PX330 vector. Table S2. Primers used to generate template DNA for in vitro transcription to produce sgRNA and mRNA form of Cas9. Table S3. Primers used to genotype IL2RG mutations introduced by CRISPR/Cas9 system. The extend primers were used to genotype IL2RG from fetus 3 and 6. Table S4. The mutation of fetus. Two fetus contained hemizygous mutation in IL2RG, other two fetus had biallelic mutation, and 2 fetus had presumable large deletion (>1.9kb). (DOCX 22 kb)