RT Journal Article SR Electronic(1) A1 Doceul, Virginie A1 Charleston, Bryan A1 Crooke, Helen A1 Reid, Elizabeth A1 Powell, Penny P. A1 Seago, Julian YR 2008 T1 The Npro product of classical swine fever virus interacts with IκBα, the NF-κB inhibitor JF Journal of General Virology, VO 89 IS 8 SP 1881 OP 1889 DO https://doi.org/10.1099/vir.0.83643-0 PB Microbiology Society, SN 0022-1317, AB Classical swine fever virus (CSFV) belongs to the genus Pestivirus and is the causative agent of classical swine fever, a haemorrhagic disease of pigs. The virus replicates in host cells without activating interferon (IFN) production and has been reported to be an antagonist of double-stranded RNA-induced apoptosis. The N-terminal protease (Npro) of CSFV is responsible for this evasion of the host innate immune response. In order to identify cellular proteins that interact with the Npro product of CSFV, a yeast two-hybrid screen of a human library was carried out, which identified IκBα, the inhibitor of NF-κB, a transcription factor involved in the control of apoptosis, the immune response and IFN production. The Npro–IκBα interaction was confirmed using yeast two-hybrid analysis and additional co-precipitation assays. It was also shown that Npro localizes to both the cytoplasmic and nuclear compartments in stably transfected cells and in CSFV-infected cells. Following stimulation by tumour necrosis factor alpha, PK-15 cell lines expressing Npro exhibited transient nuclear accumulation of pIκBα, but no effect of CSFV infection on IκBα localization or NF-κB p65 activation was observed., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.83643-0