The membranous bound and pigment containing bodies present in the cell are called plastids. Name plastid was first used by Schimper in 1883. Plastids are present only in plant cells and some algae. Plastids are semi autonomous bodies of cells. They contain their own DNA and ribosome. So they are self replicating organelles. The newly form plastid is called protoplastid. It then changes into Elastoplast. Etioplast grows to form complete plastids. The plastids are transferred to next generation through cytoplasm of egg. This plastid then divides to form many RCS% plastids. Plastids are absent in sperm. There are three types of plastids. chloroplasts, chroinolast and leucoplast:

A. Chloroplast

The membrane bound structure, containing green pigment chlorophyll is called chloroplast. The chloroplasts are present only in photosynthetic plants and algae. They are present in leaves, green stems and unripened fruit. But they are present most abundantly in the mesophyll cells of leaves. There are about half a million chloroplasts per square millimeter of leaf surface. A typical mesophyll cell contains about 30 to 40 chloroplasts. According to one observation, leaf of Ricinus contains 400,000 chloroplasts per square centimeter. They size of chloroplast is 2 — 4 IA in width and 4 — 71.t in length. Chloroplasts are self replicating organelles.

The shape of chloroplast is different in different species of plants. Flick shapes may be spheroid. ovoid or discoid. Some chloroplasts are vesicle like. Starch grains are present in chloroplast. Light .affect the distribution of chloroplasts in cell. Chloroplast concentrates towards the light source.

The green pigment is an organic compound called chlorophyll. Chloroplast also contains carotenoids. It helps the plant to absorb light energy and manufacture food. The central atom of chlorophyll is Mg. Chloroplasts are present in .the form of protoplastids in cotyledon and primordium of stein. They are colorless at first. But they develop chlorophyll and become green.12

Structure of Chloroplast

chloroplast has different shapes and structures. Its diameter is abo4it 4 — 6 pm. It appears heterogeneous structure under light microscope. It has small granule like structures called grana. ‘these grana are embedded in the matrix. 1 he chloroplast shows three main components under electron microscope. ‘these are envelope. stroma. thylakoid and granum.



  1. The Envelope: It is outer covering of the chloroplast. It formed
    by a double membrane. The outer membrane is smooth. But inner membrane extends inward. At some places, the inner membrane is in continuous w it Ii the thy lakoids. ‘Flits contact isused for transfer of material into and out of the chloroplast tocytosol: Inner membrane also contain small amount of
  2. 12


  1. Stroma: Stroma is a fluid that surrounds the thylakoids. It covers most of the o tunic of the chloroplast. It is gel like substance. It contains about 50 % of the chlorophst proteins. It contains proteins, some ribosomes and a small circular DNA. Calvin cycle or dark reaction takes place in stroma. The CO, is fixed and sugars are synthesized in stroma. Some proteins are also synthesized in this part.
  2. Thylakoids: These are flattened vesicles. The outer surface of thylakoids is in contact with the stroma. Its inner surface encloses intra-thylakoids space. The number of thylakoids varies from 12 to 40. Thylakoids are membranous structure. Their membrane is composed of 50% lipids and 50 % proteins. The lipids are mainly glycolipids, sulpholipids and phospholipids. The chlorophyll molecules are arranged on the layers of thylakoids. Chlorophyll molecules are present in the form of clusters. They form photosystems. There are two types of photosystems: photosystin I and photosystem II. The electroncarrier of the electror transport chain is present in the membrane of thylakoids. These electron carriers export the Fr across the thylakoids. It creates Er gradient. These protons come back through ATPase enzyme. It synthesizes ATP. Thylakoids are stacked to form grana and intergrana.
    1. Granum: A granum is pile of thylakoids, stacked on each other like coins. On the average, 50 or more thylakoids are piled to form one granum. The light energy is trapped by the membranes of grana and ATP is synthesized.
    2. Intergranum: The grana are interconnected with each other by the non-green part, called intergranum.


B. Chromoplast

The plastids containing coloured pigments other than chlorophyll are called chromolast. Chromoplasts give colour to plants other than green. Their shapes may be triangular or pyramidal or elliptical. They are present in the petals of flowers, and in the ripened fruits. They are not actively involved in photosynthesis. They help in pollination and dispersal of seeds. They are themselves formed from chloroplast or leucoplast. The chloroplast of ripened fruit develops coloured pigments and change into chromoplast. That is why the colours of ripened fruits changes from green to yellow,

orange, red etc. Red pigment in chromoplast is lycopene. It is a member of carotenoids family. It is present in the ripened fruits of mangoes, tomatoes etc. The chromoplast of algae contains red pigment phycoerythirn and blue pigment phycocyanin.

C. Leucoplasts

The colourless plastids are called leucoplasts. Their shapes may be oval, tubular or some other. They are found in the underground parts like root of the plant and store food. They are also found in embryonic, germs cells and meristematid cells. Thylakoids are absent in leucoplast. There are three forms of leucoplasts:

  1. Amyloplasts: The starch storing leucoplasts are called amyloplasts.
  2. Aleuroplast: The protein storing leucoplasts are called aleuroplast.
  3. Lipoplast: The lipid storing leucoplasts are called lipoplast.

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