databaseArrayExpressfile_versionsfilesTxt Reina GarciaSaška IvanovaRubí Zamudio-VázquezspeciesHomo Sapienssubmitter_keywordstranscription profiling by arrayfull_dataset_link Protocol - The HeLa cervical adenocarcinoma cells were grown in DMEM supplemented with 10% fetal bovine serum (FBS), 2 mM L-glutamine and antibiotics, and maintained at 37 ºC in a humidified atmosphere containing 5% CO2. Sample Treatment - 24 h after sub-culturing cells were completely attached to the plate's surface. A final concentration of 5 μM RZ2 was added to the medium and treated cells were incubated for another 24 h. The same DMSO volume used as RZ2 solvent vehicle was used as control. Hybridization - Hybridization mixture was prepared according to Affymetrix protocol. Each sample was hybridized to a GeneChip PrimeView Human Gene Expression Array (Affymetrix). Labeling - RNA expression profiling was performed following the Pico Profiling method (Gonzalez-Roca et al., PLoS One, 2010), from 25 ng total RNA. Nucleic Acid Extraction - Cells were washed with PBS and then lysed using a buffer containing β-mercaptoethanol. Isolation steps are done following PureLink® RNA Mini Kit technical manual. The optional step of DNAse treatment was performed Scaning - Arrays were washed and stained in a Fluidics Station 450 and scanned in a GeneChip Scanner 3000 (both Affymetrix) according to manufacturer’s recommendations.data_protocolData Transformation - Arrays were processed in Bioconductor, using RMA background correction and summarization. Foldchanges between samples were computed after MA mean and variance normalization using the GAM method. An empirical Bayes partial density model was then used to identify significant differentially expressed genes with a False Discovery Rate (FDR) of 5% and a log2 foldchange threshold of 3 (8 times up or down regulated). ID_REF = VALUE = log2 RMA signal intensitysample_synonymsRibonucleic, DIMETHYL SULFOXIDE, Fanconi syndrome with intestinal malabsorption and galactose intolerance, affixed to, nutrient medium, C5H10N2O3, antimicrobial agents, 身体表面, Homo sapiense, L-(+)-glutamine, Adenocarcinoma of the Cervix Uteri, Uterine Cervix Adenocarcinoma, Ethanol, KL receptor activity, L-glutamine, microbicides, Gsfsco1, L-Glutaminsaeure-5-amid, Homo sapien, Method, Homo sapians, SCO5, L-2-aminoglutaramic acid, SCO1, GSD due to GLUT2 deficiency, Gsfsow3, Gsfsco5, Non Polyadenylated, RNA Gene Products, integumentum commune, SOW3, 人(human being), antimicrobial, treatment, NEOPL CERVICAL, Homo sapients, Gene Expressions, reference sample, sarcoma of breast, Antibiotika, PTPSTEP, Q, dimethyl sulphoxide, 2 Mercaptoethanol, Adenocarcinoma of Cervix, purification, W, Glycogenosis due to GLUT2 deficiency, Abdominal muscle deficiency syndrome, Adenocarcinoma of Uterine Cervix, Pico, isolation and purification, h, Methodological Studies, Homo sapience, DNAse, sample, n, CERVIX NEOPL, disease management, 人(man), Bs, RCB0007, Acz, Sl, Home sapiens, Cervix Cancer, Dimethylsulfoxid, antibiotic, CERVICAL NEOPL, Entire integumentary system, DMSO, external covering of organism, dimethyli sulfoxidum, total RNA extract, UTERINE CERVICAL NEOPL, dmso, ribose nucleic acid, Eagle-Barret syndrome, 生物体表面, methylsulfinylmethane, ribonucleic acids, Tr-kit, organism surface, Adenocarcinoma of the Cervix, Procedure, breast sarcoma, Uterine, glycogen storage disease 11, PBS, PBT, (CH3)2SO, Cervical Cancer, Homo sapian, Uterine Cervical, Ribonukleinsaeure, hepatic glycogenosis with Fanconi Nephropathy,, pentosenucleic acids, microbicide, Ribonucleic acids, Bickel-Fanconi glycogenosis, Obrisnksy syndrome, KIT, tyrosine-protein kinase Kit, Acid, glycogenosis due to GLUT2 deficiency, absence of, (methanesulfinyl)methane, glycogen storage disease due to GLUT2 deficiency, Painful Bladder Syndrome, medium, Step, glycogenosis Fanconi EXACT, DMEM, abdominal muscle deficiency syndrome, Striatum-enriched protein-tyrosine phosphatase, Methodological, (S)-2, study protocol, Homo sapines, Methodological Study, Cervix Uteri Adenocarcinoma, human, Fanconi-Bickel syndrome, L-glutamic acid gamma-amide, Adenocarcinoma of Cervix Uteri, Atmospheres, Uterine Cervical Cancers, Painful bladder syndrome, hepatic glycogenosis with amino aciduria and glucosuria, integumentary system, STEP, 2-Mercaptoethanol, microarray, Uterine Cervical Neoplasm, Fanconi type, GSD type XI, dermal system, triad syndrome, 真皮系统, 生物体的外部覆盖, GLUT2 deficiency, GLUTAMINE, 2-ME, human being, Procedures, antibiotique, sarcoma of the breast, 5-diamino-5-oxopentanoic acid, Neoplasms, 酪氨酸蛋白激酶试剂盒, Cervix Adenocarcinoma, SCF receptor activity, Levoglutamide, dimethylsulfoxyde, Gene, FBS, C2H6OS, (2S)-2-amino-4-carbamoylbutanoic acid, abdominal muscles, surface, method, Homo spaiens, Fröhlich syndrome, isolation, method used in an experiment, Gene Products, Studies, 原癌基因c-Kit, Fanconi Bickel syndrome, Cervical Neoplasm, “人” EXACT genbank_common_name [], SCFR, pseudo-phlorizin diabetes, antibiotics, S(O)Me2, sulfinylbis(methane), Fdc, Congenital absence of abdominal muscles, (2S)-2, HeLa, labeling, man, Expressions, Cervical Neoplasms, Non-Polyadenylated RNA, Study, dimetil sulfoxido, Neural-specific protein-tyrosine phosphatase, glycogen storage disease type 11, Homo sampiens, prune belly syndrome, L-Glutamine, Expression, Cancer of Cervix, Cervix Neoplasm, Controlled, Cancer, D-MEM, Antibiotikum, proto-oncogene c-Kit, Controlling, RNA, Cervix Neoplasms, eagle-Barret syndrome, Fanconi-Bickel disease, 人(human), RNS, L-2-Aminoglutaramic acid, buffer, Cell, Cancer of the Uterine Cervix, disease management., KIT ligand receptor activity, yeast nucleic acid, glycogen storage disease XI, Cervical Cancers, Neoplasm, Urethral obstruction sequence, Glutamic acid 5-amide, L-Glutamin, Dimethyl sulfoxide, Homo sapeins, NEOPL CERVIX, attached, eagle-Barrett syndrome, glycogen storage disease type XI, glycogenosis, syndrome of agenesis of abdominal muscles, Uterine Cervical Neoplasms, ribonucleic acid, hepatorenal glycogenosis with renal Fanconi syndrome, c-KIT, with urinary tract Abnormality and cryptorchidism, GSD type 11, Glutamic acid amide, body surface, growth medium, Non Polyadenylated RNA, 外皮系统, Cancer of the Cervix, Non-Polyadenylated, dimethyl sulfoxide, Humo sapiens, Uterine Cervical Cancer, Cancers, Ribonucleic Acid, CD117, FBS1, sample population, Obrinsky syndrome, plan specification, Homo spiens, "human" EXACT genbank_common_name [], hepatorenal glycogenosis with renal fanconi syndrome, 10^[-12], C-Kit, concentration, 表面, Cervix, Ssm, 2-mercapto-, Cervical, dimethyl sulfur oxide, Adenocarcinoma of the Uterine Cervixdescription_synonymsmalignant Growth, Ribonucleic, DIMETHYL SULFOXIDE, human being, Macroautophagy, cell type cancer, caspase-dependent programmed cell death, Gene, dimethylsulfoxyde, C2H6OS, broad, Homo sapiense, apoptotic programmed cell death, type I programmed cell death, activation of apoptosis, 凋亡细胞死亡, autophagy, 半胱氨酸蛋白酶依赖的程序性细胞死亡, Homo spaiens, Homo sapien, Autophagic Cell Deaths, Extrinsic Pathway Apoptoses, Homo sapians, Cellular Autophagies, Gene Products, Cellular Autophagy, Type I, “人” EXACT genbank_common_name [], neoplasm, Macro-autophagy, Non Polyadenylated, malignant tumour, RNA Gene Products, 人(human being), S(O)Me2, sulfinylbis(methane), Homo sapients, Gene Expressions, Autophagies, 信号（启动子）半胱氨酸蛋白酶活性, Genomes, Macro autophagy, 凋亡程序性细胞死亡, neoplasm (disease), HeLa, Macro-autophagies, dimethyl sulphoxide, malignant neoplasia, Deaths, apoptosis activator activity, man, Expressions, CA, Non-Polyadenylated RNA, 致力于细胞凋亡, dimetil sulfoxido, 细胞凋亡诱导, Intrinsic Pathway Apoptosis, h, Homo sapience, malignant neoplasm, Homo sampiens, execution phase of apoptotic process, accidental cell death, 人(man), necrosis, Expression, programmed cell death by apoptosis, Home sapiens, Dimethylsulfoxid, Cancer, Apoptosis, 细胞凋亡, cellular suicide, Macroautophagies, malignancy, 凋亡激活剂活性, RNA, DMSO, cell suicide, Malignant Neoplasm, wide/broad, Autophagic, dimethyli sulfoxidum, dmso, ribose nucleic acid, malignant, apoptotic cell death, methylsulfinylmethane, 人(human), ribonucleic acids, RNS, Cell, Autophagic Cell, (CH3)2SO, MT, signaling (initiator) caspase activity, induction of apoptosis, HeLa Cell, microarray., Homo sapian, Intrinsic Pathway, yeast nucleic acid, malignant neoplasm (disease), Programmed Cell Death, Extrinsic Pathway Apoptosis, Ribonukleinsaeure, Autophagic Programmed Cell Death, organ system cancer, Autophagocytosis, Cellular, Autophagic Cell Death, pentosenucleic acids, 凋亡信号, 细胞自杀(cell suicide), Ribonucleic acids, Homo sapeins, Dimethyl sulfoxide, Death, Cell Deaths, I型程序性细胞死亡, apoptosis signaling, ribonucleic acid, Acid, (methanesulfinyl)methane, primary cancer, apoptosis, Autophagy, Non Polyadenylated RNA, Non-Polyadenylated, Humo sapiens, 细胞凋亡激活, dimethyl sulfoxide, Cancers, Cell Death, whole genome, Ribonucleic Acid, Homo sapines, 通过p53诱导细胞凋亡, malignant tumor, human, 细胞自杀(cellular suicide), Intrinsic Pathway Apoptoses, wide, Homo spiens, "human" EXACT genbank_common_name [], induction of apoptosis by p53, malignant neoplastic disease, apoptotic program, Cells, Extrinsic Pathway, commitment to apoptosis, microarray, 凋亡引发的程序性细胞死亡, Type II, dimethyl sulfur oxide, cancer, Apoptoses, 凋亡程序data_synonymsALY|REF, GLS1, negative regulation of adrenergic receptor signalling pathway, proportion, Materials, Genetic, Procedures, ALY, AAD20, proportionality, Gene, rate, Methodological, Procedure, Methodological Study, Cistrons, introduction, plan specification, Study, BEF, data analysis, KGA, method, density, data processing, GAC, Methodological Studies, false, Material, Method, method used in an experiment, THOC4, regulation of adrenergic receptor signalling pathway, Studies, quotient, signal peptide, 信号肽, GAM, Genetic Materials, REF, Cistron, background, Genetic Material, 信号, signal., rationame_synonymsHeLa, 数据, data, HeLa Cell, HeLa., Cell, Cellsview_count8isClaimabletruecitation_count0search_count1search_domainspatentfamilies~0rfam~0merops~0complex-portal~0uniprot~0wormbaseparasite~0reactome~0emdb~0wgs_masters~0ebiweb_resources~0biomodels_all~0ipd-mhc~0genome_assembly~0taxonomy~1ebiweb_people~0enzymeportal_enzymes~0pdbe~0chebi~0patentproteins~0interpro7~0uniref~0chembl~0gpcrdb~0hgnc~0intact~0rhea~0imgt-hla~0patentnucleotides~0ensemblroot~0non-coding~0europepmc~0identifiers_registry~0pdbechem~0biosamples~0biotools~0mesh~0coding~0sra~0efo~0project~0human_diseases~0embl~0treefam~0uniparc~0ols~0dgva~0intenz~0go~0tsa_masters~0ebiweb_corporate~0omim~0sbo~0lrg~0ipd-kir~0rnacentral~0orcid_data_claims~0metagenomics~0pfam~0citation_count_scaled0.0reanalysis_count_scaled0.0view_count_scaled0.0024984384759525295download_count_scaled6.956146712090305E-6download_count4additional_accessionGSE51948is_claimabletruenameExpression data from HeLa cells treated with RZ2descriptionThe newly sinthesized compound RZ2 induces cell death to human cancer cells. To obtain insights into its mechanism of action, the effects on autophagy and apoptosis were examined. Global perturbations in genome-wide RNA expression of HeLa cells treated with RZ2 were measured by gene expression microarray. Confluent cultures of HeLa cells were treated with 5 μM RZ2 or the vehicle (DMSO) for 24 hours, followed by RNA preparation and hybridization to a GeneChip PrimeView Human Gene Expression Array (Affymetrix)datespublication2015-05-19updated2015-05-24accessionE-GEOD-51948cross_referencesTAXONOMY9606