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Thermodynamic study of the enantiomeric resolution of flurbiprofen by HPLC using Chiralpak AD-RH column.

The chiral resolution of (+/-)-flurbiprofen was achieved using water-acetonitrile (60:40, v/v) containing 0.1% acetic acid on a Chiralpak AD-RH column at 20 degrees C. The enantio-resolution was studied with different percentages of acetonitrile. Thermodynamic parameters (enthalpy, entropy and free energy) were calculated by carrying out the enantio-resolution experiments at 0 to 60 degrees C. The enantio-resolution was found to be exothermic in nature. Attempts have been made to explain the mechanism of chiral resolution of flurbiprofen on the Chiralpak AD-RH column.