{'fraction_non_alphanumeric': 0.008605851979345954, 'fraction_numerical': 0.006024096385542169, 'mean_word_length': 6.048484848484849, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Cartilage development disorder of bone growth plate in vitamin D deficient, calcium deficient chicken

Objective To observe the chondrocyte development disorder caused by vitamin D (VitD) and calcium deficiency Methods Chondrocyte apoptosis was documented by using the technique of in situ end labeling, flow cytometric analysis and DNA fragmentation Chondrocyte morphologic changes were observed by H E staining Results The results showed that 34 7 % of the normal hypertrophic chondrocytes were undergoing apoptosis, the percentage and the location of apoptosis between the calcium deficient group and the control group had no significant difference, but in VitD deficient group the percentage of hypertrophic chondrocytes apoptosis was significantly lower (19 77%) It was also found that there were chondrocytes architectural disarrangement in Vit D deficient group Conclusion The study demonstrated that there are different mechanism of bone growth disorder between VitD and calcium deficient chicken Significantly lower hypertrophic chondrocytes apoptosis may be the main reason of chondrocytes accumulation and cartilage thickning caused by VitD deficiency