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Growth, development and chemical composition of bush tea(Athraxia Phylicoides L.) as affected by seasonal nitrogen, phosporus, and potassium nutrition

Bush tea (Athrixia phylicoides L.) is an herbaceous plant that belongs to the Asteraceae family. It has predominantly been used throughout history as a medicinal herbal tea by people of South Africa. Many studies have revealed that the plant has the commercial potential to be used as a medicinal herbal beverage. The chemical profile of wild bush tea such as flavonols and total polyphenols are not yet established. Therefore, an experiment to identify the major compound in bush tea was initiated. Matured leaves were harvested in Muhuyu village (Limpopo Province) for extraction. The green leaves were cold extracted with acetone for seven days. The extract was filtered and evaporated at 50 °C under reduced pressure to yield 312 g of a green viscous liquid. Thin layer chromatography plates were visualized under UV light (240 nm) or by spraying with visualizing reagent (anisaldehyde reagent) which was made up by mixing 250 mL ethanol, 2,4 mL concentrated sulphuric acid and 6 ml University of Pretoria etd Mudau, F N (2006)