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Increase of External Osmolarity Reduces Morphogenetic Defects and Accumulation of Chitin in agas1 Mutant of Saccharomyces cerevisiae

ABSTRACT We have performed a physiological analysis of the effects of high-osmolarity media on gas1Δ cells. The reductions in the duplication time, number of pluribudded cells, hypersensitivity to Calcofluor and sodium dodecyl sulfate, and chitin level indicate a partial suppression of the mutant phenotype. GAS1 deletion was found to be lethal in the absence of the Bck1 and Slt2 (Mpk1) proteins of the cell integrity pathway.