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A cell separation chip using micro-structures filter and multi-frequencies dielectrophoresis

In this paper, we report the development of a dielectrophoresis cell separation chip and present the preliminary results. Both dielectrophoresis force and size filtering microstructure are incorporated in our microchip for the function of cell separation. By applying individual input voltage with different frequency on the different rows of the micro-tips, different particles could be separated in our device based on their size and electric property. An additional micro-structure design filter is also adopted in this chip to function as a multi-size filter to enhance the separation efficiency. Analysis, simulation, and experimental demonstration are reported in this paper to verify the functions of our separation chip.