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Distribution of specific intermediate‐filament proteins in the goldfish retina

The intermediate‐filament proteins expressed in the goldfish retina were investigated by immunohistochemistry and by immunoblotting. Polyclonal antibodies that previously had been raised against the goldfish optic nerve neurofilament (ON1 and ON2) and glial filament (ON3 and ON4) proteins were used in this study. Anti‐ON1/ON2 antiserum reacted on a retinal immunoblot with two proteins having molecular weights and isoelectric points corresponding to those of ON1 and ON2. Histologically, the most pronounced anti‐ON1/ON2 reactivity was observed in the ganglion cell layer of the goldfish retina. The anti‐ON3/ON4 antiserum reacted with a single protein on a retinal immunoblot. This protein had a molecular weight and isoelectric point which corresponded to the goldfish optic nerve glial filament proteins. This anti‐serum labeled horizontal cells in retina sections. Three previously unidentified goldfish visual‐pathway intermediate‐filament proteins sharing a molecular weight of 60K were observed on two‐dimensional gels of retinal cytoskeletal proteins and on retinal immunoblots which were probed with a monoclonal antibody which recognizes an epitope common to all intermediate Filament proteins.