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Protocol for high‐resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle

In this short communication we describe a specific protocol for SDS‐PAGE separation of adult bovine myosin heavy‐chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini‐gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.