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Anti‐proliferative Properties of Methanolic Extracts of Annona muricata in Colon, Lung and Skin Cancer Cell Lines

Annona muricata, commonly known as Soursop, is a tree that grows in tropical climates. Traditionally, different parts of A. muricata are used to prepare teas and juices, which have been used as homemade remedies for diseases such as diabetes and cancer. Previous studies in our laboratory, showed that extracts obtained from A. muricata's leaves had anti‐proliferative activity towards HT‐29 colorectal adenocarcinoma cell. Based on these previous results, in this study we evaluate the effect of the Soursop's fruit peel extract on the proliferation of colorectal adenocarcinoma at 24‐, 48‐ and 72‐hours. In addition, we explored if this extract affects the proliferation of non‐malignant CCD‐1074sk (epithelial) and MRC‐5 (fibroblast) cell lines using the same experimental design. We hypothesized that Soursop's fruit peel extracts will inhibit proliferation of HT‐29 colorectal adenocarcinoma but will not show affect the studied non‐malignant cell lines. We prepared our samples by performing methanolic extractions of the fruit's peel. For the proliferation assays we seeded the HT‐29, CCD‐1074 and MCR‐5 cells in 96‐well plates and after an overnight recovery period, we applied five treatments consisting of concentrations of the extracts: 400ug/mL, 300ug/mL, 200ug/mL, 100ug/mL, and 50ug/mL and DMSO at 1,43% final concentration as our control vehicle. Our preliminary results on the HT‐29 cells showed that at 24‐hours post treatment, only the highest concentration (400 ug/mL) presented a significant inhibition (p<0.05) at 48‐hours we observed inhibition from 50ug/mL to 400ug/mL and at 72‐hours we observed inhibition at all experimental concentrations. For the non‐malignant cell lines CCD‐1097and MRC‐5 the extract did not affect the proliferation of these when compared to their respective vehicle controls