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Molecular Identification of Lactic acid bacteria Isolated from Lactating Cattle Lower Gut as a Potential Probiotic Species

Balanced diet is a basic factor in livestock productivity. There is still insufficient knowledge on indigenous probiotic species of livestock gastrointestinal tract. The present experiment was conducted for isolation and molecular identification of probiotic bacterial species from cattle lower gut and to determine their probiotic potential. Twenty (20) bacterial isolates were identified morphological and biochemical methods. Out of twenty isolates, three strains (PBC-3, PBC-6 and PBC-9) were selected as presumptive probiotic strains based on their enzymatic potential (cellulolytic, amylolytic and proteolytic ability), survival ability in low pH, better hydrophobicity, auto-aggregation, gamma haemolytic activity and better results against antimicrobial species. These isolates were identified as Enterococcus faecalis (PBC-3), Pediococcus acidilacticii (PBC-6) and Pediococcus pentosaceus (PBC-9) by 16S rRNA gene sequencing. Results of present study illustrated that Pediococcus acidilacticii (PBC-6) and Pediococcus pentosaceus (PBC-9) bacterial strains showed best probiotic potential and can be used in cattle feed in future