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Activation of the Canonical Wnt/β-Catenin Pathway in ATF3-Induced Mammary Tumors

Female transgenic mice that constitutively overexpress the transcription factor ATF3 in the basal epithelium of the mammary gland develop mammary carcinomas with high frequency, but only if allowed to mate and raise pups early in life. This transgenic mouse model system reproduces some features of human breast cancer in that about 20% of human breast tumor specimens exhibit overexpression of ATF3 in the tumor cells. The ATF3-induced mouse tumors are phenotypically similar to mammary tumors induced by overexpression of activating Wnt/β-catenin pathway genes. We now show that the Wnt/β-catenin pathway is indeed activated in ATF3-induced tumors. β-catenin is transcriptionally up-regulated in the tumors, and high levels of nuclear β-catenin are seen in tumor cells. A reporter gene for Wnt/β-catenin pathway activity, TOPGAL, is up-regulated in the tumors and several downstream targets of Wnt signaling, including Ccnd1, Jun, Axin2 and Dkk4, are also expressed at higher levels in ATF3-induced tumors compared to mammary glands of transgenic females. Several positive-acting ligands for this pathway, including Wnt3, Wnt3a, Wnt7b, and Wnt5a, are significantly overexpressed in tumor tissue, and mRNA for Wnt3 is about 5-fold more abundant in transgenic mammary tissue than in non-transgenic mammary tissue. Two known transcriptional targets of ATF3, Snai1 and Snai2, are also overexpressed in the tumors, and Snail and Slug proteins are found to be located primarily in the nuclei of tumor cells. In vitro knockdown of Atf3 expression results in significant decreases in expression of Wnt7b, Tcf7, Snai2 and Jun, suggesting that these genes may be direct transcriptional targets of ATF3 protein. By chromatin immunoprecipitation analysis, both ATF3 and JUN proteins appear to bind to a particular subclass of AP-1 sites upstream of the transcriptional start sites of each of these genes.


Introduction
The bZip transcription factor, ATF3, has for some time been known to have the potential to both activate and repress transcription in a context-dependent manner [1][2][3][4][5]. ATF3 homodimers appear to be repressors [5,6], whereas heterodimers with Jun or JunB activate gene expression [3]. Although ATF3 expression has clearly been associated with the DNA damage response and responses to other cellular stressors [2,[7][8][9][10], its exact role in these processes remains unclear. In some systems, ATF3 appears to function in tumor suppressive pathways [11,12], and in particular has recently been associated with induction of apoptosis after treatments that induce lethal levels of DNA damage [13].
On the other hand, ATF3 has been shown to be growth stimulatory or anti-apoptotic in several systems [1,[14][15][16][17][18][19]. Several recent studies implicate ATF3 in tumorigenesis. In a transgenic mouse model in which human ATF3 is expressed from the bovine cytokeratin 5 promoter (BK5.ATF3), we reported spontaneous development of oral tumors, including about a 70% incidence of squamous cell carcinomas at 16 months of age [20]. We recently demonstrated that in this same model, parous female transgenic mice develop mammary tumors with squamous differentiation in the first year of life with an incidence of about 67% [21]. Although the transgene is expressed in the basal, myoepithelial compartment of the mammary gland throughout development, mammary tumors do not develop in virgin, transgenic females. Hai and colleagues have demonstrated overexpression of the ATF3 protein in a majority of human breast cancers [19], and we have found by immunohistochemistry (IHC) that in about 20% of human breast cancers ATF3 is found to be localized to the nuclei of the tumor cells; in the remaining ATF3-positive breast cancers, expression is primarily seen in stromal elements [21].
Phenotypically, mammary tumors induced in the BK5.ATF3 model resemble tumors and dysplastic lesions that have been reported in several transgenic models in which components of the Wnt/b-catenin pathway are overexpressed and the pathway is thereby constitutively activated [22][23][24][25][26][27][28]. For example, mammary tumors that arise in mice expressing a stabilized form of b-catenin from the cytokeratin 14 promoter express differentiation markers characteristic of both mammary epithelium, including cytokeratin 8, and epidermis, including cytokeratin 10 [28]. We have found aberrant expression of cytokeratins 5 and 8 in most tumor cells in the BK5.ATF3 model [21], as well as supra-basal expression of cytokeratins 6 and 10 and several cytokeratins that are characteristic of the inner root sheath of hair follicles. Interestingly, nuclear IHC staining for the ATF3 transgene is confined to the basal cell layer in the tumors [21]. In both types of models, squamous metaplastic histopathology is seen, with the tendency to form cyst-like structures with a core of keratinaceous material and cell debris, surrounded by a multi-layered epithelium that exhibits several features of squamous differentiation. These phenotypic similarities suggested the possibility that the Wnt/b-catenin pathway is somehow activated in ATF3-induced mammary tumors, or alternatively that ATF3 is a downstream effector of Wnt/b-catenin signaling. However, functional links between ATF3 and Wnt/b-catenin signaling have not been described in the literature.
The Wnt/b-catenin pathway is well known for its involvement in colon carcinogenesis [29]. About 85% of both familial and sporadic colon cancers involve mutations in the APC gene that lead to activation of the Wnt/b-catenin pathway [30]. Wnt/b-catenin signaling is absolutely required for mammary gland development, and acts at several critical time periods during pre-and post-natal development. Over the past decade, several epigenetic abnormalities in Wnt pathway genes have also been identified in human breast cancer. Promoter methylation of the APC gene has been found in about 40% of breast cancer cases [31,32], and high levels of promoter methylation for several Wnt-inhibitory genes in the SFRP and DKK families in breast cancer have also been reported [33,34].
The canonical pathway of Wnt/b-catenin signaling [35] begins with the interaction of an extracellular Wnt family protein with a transmembrane receptor of the Fz family; each of these gene families have more than a dozen members in mouse and human. This triggers formation of a complex with a second membrane coreceptor, Lrp5 or Lrp6, phosphorylation of both receptors, and binding of two cytoplasmic proteins, Disheveled and Axin, to the complex. In the unstimulated cell, cytoplasmic b-catenin associates with a so-called destruction complex, containing the proteins Axin, APC and Gsk3. In this complex, b-catenin is specifically phosphorylated by the kinase activity of Gsk3, which marks it for subsequent ubiquitylation and degradation by the proteasome. Following Wnt binding to the receptor, the destruction complex becomes tethered to the membrane ligand/receptor complex through Axin and loses its affinity for b-catenin, which then accumulates in the cytoplasm and is transported to the nucleus. Transcription factors of the Tcf/Lef1 family in the nucleus are then converted from repressors to activators by replacement of a repressive partner, Groucho, with the activating partner, bcatenin, thus activating transcription of an array of genes that constitute the downstream mediators of the pathway. In many systems, the pathway can be activated by adding exogenous Wnt protein, or by inhibiting the activity of Gsk3.
In the present study, we show that the Wnt/b-catenin pathway is functionally activated in mammary tumors induced in the BK5.ATF3 model. Initial probing of possible molecular links between ATF3 expression and Wnt/b-catenin identified robust up-regulation in ATF3-induced tumors of Wnt3, Wnt3a, Wnt10b and Wnt7b, several activating ligands for the canonical pathway.

Results
b-catenin is overexpressed in and localizes to the nuclei of BK5.ATF3 tumor cells In a previous study, similarities were noted between the histopathology of ATF3-induced mammary tumors and lesions described in several transgenic models in which the Wnt/bcatenin pathway is upregulated. Notably, expression of a stabilized form of b-catenin in basal cells from the Krt14 promoter resulted in squamous metaplastic lesions and carcinomas, similar to the pathology seen in BK5.ATF3 mammary tumors [28]; similarities in aberrant patterns of cytokeratin expression were also seen between these two models. However, there is no precedent in the literature for an effect of ATF3 on the Wnt/b-catenin pathway. To determine whether Wnt/b-catenin signaling is affected in the BK5.ATF3 mammary tumorigenesis model, we first used IHC to analyze the distribution of b-catenin in mammary glands and in ATF3-induced tumors. In the absence of Wnt/b-catenin pathway activation, b-catenin is important in maintaining epithelial intercellular communication [35] and is therefore found in normal mammary duct epithelial cells, typically showing a diffuse cytoplasmic distribution or localization to the basolateral plasma membrane in glands from either BK5.ATF3 transgenic ( Figure 1A) or non-transgenic ( Figure 1B) animals. Even in glands from older, parous females, where b-catenin staining was heavier ( Figure 1C) normal ducts did not exhibit evidence of nuclear localization of bcatenin. In control sections with normal rabbit IgG substituted for the anti-b-catenin antibody, epithelial cells did not stain appreciably for b-catenin ( Figure 1D). Nuclear localization of bcatenin is characteristic of canonical Wnt b-catenin pathway activation [35]. In ATF3-induced mammary tumors ( Figure 1E,F), overall expression of b-catenin appeared to be stronger than in normal tissue, and clear localization to the nuclei of tumor cells was widespread. Nuclear b-catenin was seen extensively in the basal layers of the tumors (arrows, Figure 1E,F), but was also seen in supra-basal cells (arrowheads, Figure 1E,F). In ten independent tumors examined in detail, an average of 43% of tumor cells exhibited clear nuclear expression of b-catenin. Nuclear labeling of tumor cells was abolished when normal rabbit IgG was substituted for the specific b-catenin antibody ( Figure 1G).
To confirm this finding, mRNA was prepared from normal mammary glands of young adult virgin females, ATF3-induced mammary tumors, and MMTV.neu-induced mammary tumors, and analyzed by quantitative polymerase chain reaction (qPCR). b-catenin mRNA was detected at similar levels in normal mammary tissue from non-transgenic mice and BK5.ATF3 heterozygous mice (Figure 2A). Seven independent ATF3-induced tumors exhibited an average of about three-fold higher levels of bcatenin mRNA as compared to normal mammary glands from BK5.ATF3 animals (t-test, p,.001). As expected, MMTV.neu induced tumors [36] had no such increase in b-catenin mRNA (p..05).
Downstream Wnt/b-catenin target genes are upregulated in BK5.ATF3 tumors To determine whether the nuclear localization of b-catenin correlated with activation of the Wnt/b-catenin pathway, we utilized a reporter strain of mice, TOPGAL [37], in which Wnt/ b-catenin signaling drives the expression of a bacterial bgalactosidase gene in a non-tissue-specific manner. Doubly transgenic BK5.ATF3 Tg/0 ;TOPGAL Tg/0 female mice, hemizygous for both transgenes, were allowed to raise two litters of pups, and then monitored for mammary tumor formation until 16 months of age. As expected, about two-thirds of these mice developed mammary tumors between the ages of 6 and 12 months. Indeed, the survival of these mice ( Figure S1A) was virtually identical to that reported for singly transgenic BK5.ATF3 mice, and the histopathology of the resulting tumors ( Figure S1B) was also unchanged by the TOPGAL transgene. By qPCR, expression of the b-galactosidase transgene was increased an average of about 7-fold in tumors produced in doubly transgenic mice compared to normal mammary glands of TOPGAL mice (Figure 2A; p,0.01). There was no significant difference in b-galactosidase expression between normal mammary glands of TOPGAL mice that did or did not carry the BK5.ATF3 transgene (p.0.05). These findings strongly indicate that the Wnt/b-catenin pathway is activated in these tumors, but not in normal mammary glands of BK5.ATF3 transgenic mice.
Analysis of bacterial b-galactosidase expression by IHC was complicated by background cross-reactivity in ATF3-tumors induced in non-TOPGAL mice ( Figure 3A). It can be seen that modest but fairly uniform staining was seen in the supra-basal tumor cells (arrows) but not in the basal cell layer of the tumors (asterisks). The antibody used for IHC is known to cross-react with mouse b-galactosidase, suggesting that the mouse protein may be present in the supra-basal layers of these tumors. In contrast, Figure 1. IHC analysis of b-catenin in ATF3-induced mammary tumors. b-catenin expression was assessed by immunohistochemistry in normal mammary glands of: A, young (18 weeks), non-parous BK5.ATF3 females; B, young (18 weeks), non-parous non-transgenic females; or C,D, older parous non-transgenic females; in panel D, non-specific rabbit IgG was substituted for the anti-b-catenin antibody as a negative control. Mammary tumors derived from parous BK5.ATF3 females were analyzed with the anti-b-catenin antibody (panels E, F), or with the IgG control (panel G). Arrows in panels E and F indicate typical nuclear staining of basal tumor cells, and arrowheads indicate supra-basal nuclei. Scale bar in G = 50 mm, applies to all panels. doi:10.1371/journal.pone.0016515.g001 tumors arising in BK5.ATF3 Tg/0 ;TOPGAL Tg/0 mice ( Figure 3B) demonstrated uniform staining throughout both the basal and supra-basal layers of the tumor. We conclude that activation of the Wnt/b-catenin pathway occurs at least in the basal cell layers of these tumors, coincident with nuclear expression of ATF3 [21].
As a further test of Wnt/b-catenin pathway activation, we used qPCR and protein immunoblotting to monitor activity of known downstream transcriptional targets of this pathway. Wnt/bcatenin signaling is usually associated with increased cell proliferation, and correlated with this, Ccnd1 and Jun have both been shown to be transcriptional targets of the Wnt/b-catenin pathway [38][39][40]. As shown in Figure 2B, the Ccnd1 gene was upregulated about four-fold in ATF3-induced tumors compared to normal mammary glands (p,0.001), and Jun expression was up about two-fold (p,0.001). Furthermore, at the protein level, robust up-regulation of both cyclin D1 and Jun proteins was seen in immunoblots ( Figure 2C). Interestingly, immunoprecipitation of mammary tumor extracts with ATF3-specific antiserum, followed by immunoblotting demonstrated significant intracellular association between ATF3 and Jun proteins ( Figure 2D). ATF3-Jun Figure 2. Expression analysis of Wnt/b-catenin pathway genes. Gene expression analysis was performed as described in Materials and Methods by quantitative real-time PCR for: A, Ctnnb1, bacterial b-galactosidase, Snai1; and B, Ccnd1, Jun, Axin2, and Dkk4. WT, mammary glands from non-transgenic mice; TG, mammary glands from BK5.ATF3 mice; TU, mammary tumors from BK5. ATF3 mice; Neu, mammary tumors from MMTV.neu mice. The mean and standard deviation of relative expression levels for at least 5 individual mice are shown; for all genes the mean expression level obtained with BK5.ATF3 mammary glands was set to 1.0. The statistical significance of differences between mean expression levels, calculated relative to BK5.ATF3 mammary glands, is indicated over the bars of the histograms: *, p,0.05; **, p,0.01; ***, p,0.001. C, immunoblots from a representative BK5.ATF3 mammary gland extract (M) and a mammary tumor (T) are shown for Cyclin D1, Jun and Snail; laminin A,C is shown as a protein loading control. D, extracts from paired mammary gland and tumor samples were immunoprecipitated with antibody to ATF3, then electrophoresed and immunoblotted for ATF3 and Jun. E, immunoblots of nuclear extracts from non-transgenic mammary glands (lanes 1-3), BK5.ATF3 transgenic mammary glands (lanes 4-6) or BK5.ATF3 mammary tumors (lanes 7-9) were prepared with a phospho-serine 73-Jun specific antibody; all lanes are from a single representative gel. doi:10.1371/journal.pone.0016515.g002 heterodimers have previously been shown to function as activators of gene expression, whereas ATF3 homodimers are generally thought to be repressors [3,5,6]. Jun must be phosphorylated at serine 73 in order to exhibit maximal activity as a transcriptional activator [41]. Using an antibody specific for phosphoserine-73, we noted high but variable levels of phosphorylated Jun in extracts from BK5.ATF3 transgenic mammary glands ( Figure 2E, lanes 4-6) and from BK5.ATF3-induced mammary tumors ( Figure 2E, lanes 7-9); much lower levels of activated Jun were seen in extracts from non-transgenic mammary glands ( Figure 2E, lanes 1-3).
Transcriptional activation of the Ccnd1 and Jun genes cannot be taken to be absolutely specific for the Wnt/b-catenin pathway, since both of these genes can be regulated by numerous other factors. However, several genes that are involved in the negative regulation of Wnt/b-catenin signaling, presumably as a homeostatic mechanism, are also known to be direct transcriptional targets of the pathway. Axin2, involved in negative regulation of the b-catenin protein through destabilization, is one such pathwayspecific gene, known to be up-regulated by Wnt b-catenin signaling [42]. As shown in Figure 2B, mRNA for Axin2 is expressed at four-fold higher levels in ATF3-induced tumors as compared to mammary glands (p,0.001). Dkk4, encoding a soluble protein that binds to Wnt receptors in the plasma membrane, but fails to activate downstream signaling, has also been shown to be a direct transcriptional target of Wnt/b-catenin pathway activation [43]. As shown in Figure 2B, Dkk4 mRNA is barely detectable by qPCR in normal glands, but highly upregulated in ATF3-induced tumors (p,0.01); the increase is at least 100-fold. In MMTV.neu mammary tumors in which the Wnt/b-catenin pathway is not involved, expression of Axin2 was extremely low (relative expression 0.01 compared to BK5.ATF3 mammary glands, p,0.01, data not shown), and Dkk4 was undetectable. These data strongly suggest that Wnt/b-catenin signaling is activated in BK5.ATF3 mammary tumors.

Wnt ligands are over-expressed in BK5.ATF3 tumors
Canonical activation of the Wnt/b-catenin pathway begins with the binding of an activating Wnt protein to membrane receptors, followed by downstream cytoplasmic events that lead to stabilization of b-catenin, facilitating transport to the nucleus and transcriptional activation of target genes. Several Wnt genes that activate canonical Wnt signaling, including Wnt2, Wnt5a, Wnt7b, and Wnt10b, are known to be expressed in the mammary gland during ductal development [44,45]. Thus, one possible mechanism for the activation of the Wnt b-catenin pathway by ATF3 would be direct activation of transcription of one or more Wnt genes. We analyzed the expression of 9 Wnt genes by qPCR in mammary tumors derived from parous BK5.ATF3 mice, and in normal, young adult mammary glands of non-transgenic and transgenic mice (Table 1). Five Wnt genes were significantly overexpressed in the tumors compared to transgenic mammary glands, including Wnt3, Wnt3a, Wnt5a, Wnt7b and Wnt10b. Notably, Wnt3 was up-regulated over 70-fold in ATF3-induced tumors compared to transgenic mammary glands. Importantly, the expression of Wnt3 was also 5-fold higher in transgenic glands than in non-transgenic glands. This may reflect a role for Wnt3 in the early stages of tumorigenesis in this model. None of the other Wnt genes assayed exhibited significantly higher expression in transgenic mammary glands compared to non-transgenic glands. Two genes, Wnt1 and Wnt 5b, were significantly down-regulated in ATF3-induced mammary tumors.
Snai1 and Snai2 are highly expressed in BK5.ATF3 tumors The SNAI1 and SNAI2 genes have been implicated in tumor progression and metastasis [46][47][48][49], and both were recently shown to be direct transcriptional target of ATF3 in human mammary cells [19]. The transcriptional repression activity of Snail and Slug proteins [49] is expected to positively impact the canonical Wnt/ b-catenin pathway. However, the possible involvement of these genes in the early phases of mammary tumorigenesis has not been demonstrated. IHC analysis indicated robust expression of Snail protein (the product of Snai1) in ATF3-induced mammary tumors ( Figure 4A). Nuclei of both basal and supra-basal epithelial tumor cells (arrows and arrowheads, respectively) exhibited Snail expression. In addition, scattered nuclei throughout the stroma were strongly positive for Snail expression (dotted line). Nuclear staining for Snail was not seen in control sections in which a blocking peptide for Snail1 was included in the incubation ( Figure 4B). Scattered Snail-positive nuclei were observed in normal mammary glands, in both the epithelial and stromal compartments ( Figure 4C). Essentially no expression of Snail was seen in mammary tumors that developed in MMTV.neu transgenic animals ( Figure 4D). Further quantitative analysis of five independent tumors indicated that 80-90% of tumor cell nuclei were positive for Snail expression. Consistent with this, qPCR analysis indicated about a four-fold up-regulation of Snai1 mRNA in these tumors compared to normal mammary glands in BK5.ATF3 animals (Figure 2A, p,0.001). Slug (the protein product of Snai2) was also expressed in the nuclei of tumor cells, but in this case was seen primarily in the basal cell layers of the tumors ( Figure 4E). Quantitatively, only about 28% of the tumor cells exhibited Slug expression; no staining was seen in normal

Knock-down of ATF3 decreases expression of Wnt pathway genes
Many of the changes in gene expression seen in ATF3-induced mammary tumors may not be due to direct effects of ATF3 on the transcription of the affected gene, but to indirect effects related to changes in signaling pathways. We would like to know which, if any, of the affected genes are direct targets of ATF3. As an initial approach, we utilized in vitro transfection with siRNA to knockdown Atf3 expression, and then measured the effects on the expression of potential target genes. For these experiments, we utilized a mouse mammary cancer cell line, EMT6, in which we found robust expression of the endogenous Atf3 gene in preliminary experiments. We identified three sequences from the Atf3 mRNA that could potentially serve as targets for gene silencing. As shown in Figure 5A, expression of ATF3 protein could be knocked down in EMT6 cells by treatment with two different siRNA sequences, A2 and A3, but not with a third siRNA (A1). When assayed at the mRNA level by qPCR ( Figure 5A), the lowest dose of siRNAs A2 or A3 (0.5 nM) decreased the expression level of the endogenous Atf3 mRNA by about 50% compared to treatment with a scrambled version of siRNA A1, while higher doses (1, 3, or 10 nM) gave ,90% knockdown.
In untreated EMT6 cells, we assayed expression of 15 endogenous mouse genes related to Wnt/b-catenin signaling and/or previously identified as up-regulated in ATF3-induced tumors. Expression of several genes of interest (Wnt3, Wnt3a and Dkk4) was undetectable in these cells. However, six genes identified as up-regulated in BK5.ATF3-induced tumors or transgenic mammary glands were easily detected by qPCR (Jun, Snai1, Snai2, Wnt7b, Wnt10b and Tcf7), and were chosen for further studies. When Atf3 mRNA expression was knocked down by ,50% using either siRNA A2 or A3, statistically significant 40-45% decreases in the expression of both Snai2 and Wnt7b were seen ( Figure 5B); in all cases, decreases were calculated relative to treatment with a scrambled siRNA that produced no knock-down of Atf3 expression. Expression of Tcf7 was similarly decreased by A3 under these conditions. On the other hand, expression of Wnt10b was increased significantly by Atf3 knock-down with both A2 and A3 (data not shown). With higher doses of siRNA A2, where Atf3 expression was knocked down .90%, about a 60% decrease in Jun expression and a 35% decrease in Snai1 expression were also seen ( Figure 5B). SNAI1 and SNAI2, the human orthologues of Snai1 and Snai2, have previously been shown to be direct transcriptional targets of ATF3 [19], but transcriptional regulation of the remaining genes by ATF3 has not been described.
As noted above for ATF3-expressing mammary glands, it is possible that the high levels of expression of Atf3 and Jun in untreated EMT6 cells promote formation of Atf3:Jun heterodimers. Such heterodimers exhibit AP-1-like activity [3], activating transcription through DNA binding sites with the consensus sequence (A/G)TGA(G/C)T(C/A)A. For several genes that are highly expressed in EMT6 cells (Jun, Tcf7, Snai2, Wnt7b and Snai1), we noted the presence in the DNA sequence upstream of the transcriptional start site of one (Jun, Snai2, Snai1) or two (Tcf7, Wnt7b) close matches (Table 2) to a particular subclass of AP-1 sites (GTGA(G/C)TCA). As a preliminary test of whether binding of ATF3 and/or JUN to these AP-1 sites might be involved in their expression in EMT6 cells, we performed ChIP experiments with antibodies against either ATF3 or JUN. For each of these genes, PCR signals for the expected fragment(s) containing the putative AP-1 binding site were obtained with chromatin precipitated with either an ATF3-specific or a JUN-specific antibody ( Figure 6). The strongest PCR products in the ChIP experiment were obtained for the putative AP-1 sites upstream of Jun (panel A), Tcf7 (panels C,D) and Snai2 (panel E), for which the target sequence exactly matched the GTGA(G/C)TCA motif ( Table 2). No PCR product was visible with either antibody in an assay directed at a non-specific site upstream of Jun (panel B) that had no match to this motif. The AP-1 sites upstream of Wnt7b and Snai1 contained one to three mismatches to the consensus motif (Table 2), and the positive PCR signals obtained in the corresponding ChIP samples (Figure 6, f-h) were somewhat weaker. These results are consistent with the suggestion that Snai2, Snai1, Tcf7, Jun and Wnt7b may be direct transcriptional targets of ATF3 and JUN, possibly binding as a heterodimer to upstream enhancer elements.

Discussion
In the present communication, we have provided several lines of evidence that the canonical Wnt/b-catenin pathway is activated in BK5.ATF3 mammary tumors. Characteristic overexpression of the b-catenin protein and its appearance in nuclei of tumor cells is seen. Downstream transcriptional targets, including Ccnd1, Jun, Axin2 and Dkk4, are up-regulated, and a bacterial reporter gene that is driven by Wnt/b-catenin signaling is activated in the tumors. In addition, the genes for several positive-acting ligands for the pathway, including Wnt3, Wnt3a, Wnt7b and Wnt10b, are significantly over-expressed in tumors. Given the known importance of Wnt/b-catenin signaling in colorectal cancer [29,30], and accumulating evidence suggesting that this pathway is often dysregulated in human breast cancer [31][32][33][34]50,51], it seems likely that Wnt/b-catenin signaling is important in the genesis of mammary tumors in this mouse model. Experiments to test this suggestion by blocking Wnt/b-catenin signaling in the transgenic mice are in progress. This is consistent with results in several other transgenic models [22][23][24][25][26][27][28] in which Wnt/b-catenin pathway activation results in mammary tumors or preneoplastic lesions. Several of these exhibit the tendency for squamous differentiation that is extremely prominent within the ATF3-induced tumors.
There have been no previous reports linking ATF3 expression to Wnt/b-catenin pathway activation. However, our finding that the genes for several positive-acting Wnt ligands (Wnt3, Wnt3a, Wnt7b and Wnt10b) are up-regulated in the tumors suggests the obvious possibility that ATF3 may be acting as a direct transcriptional regulator of one or more of these ligands. Consistent with this, we find that knockdown of ATF3 expression in vitro reduces Wnt7b expression and ChIP analysis in EMT6 cells suggests that both AFT3 & JUN proteins bind to regions upstream of the Wnt7b promoter that contain putative AP-1 consensus motifs. Other positive ligands, particularly Wnt3 and Wnt3a which are highly upregulated in ATF3-induced tumors, may also be important in tumorigenesis, but are expressed at very low levels in EMT6 cells and therefore could not be tested in the knock-down experiments. This model is attractive in that it provides an explanation for the finding that both basal and supra-basal tumor cells exhibit nuclear b-catenin expression, while only basal cells over-express ATF3. Because Wnt ligands are secreted and act extra-cellularly [35], both autocrine and paracrine stimulation of the pathway in the basal and supra-basal cells, respectively, might reasonably be expected to occur, leading to the pattern of nuclear b-catenin expression seen.
Non-parous, BK5.ATF3 females do not develop mammary tumors [21], and their mammary glands do not express high levels of b-catenin mRNA ( Figure 2B), do not exhibit nuclear localization of b-catenin ( Figure 1A), and are unable to activate the TOPGAL reporter gene (Figure 2A). Thus, although ATF3 is clearly expressed throughout post-natal development in the basal cell compartment of mammary glands in BK5.ATF3 transgenic females, this overexpression by itself in not sufficient to produce tumors, nor to fully activate the Wnt/b-catenin pathway. The effects of ATF3 expression in other systems are strongly contextdependent [3,19], and can include both apoptosis [13] and growth stimulation [14][15][16][17], oncogenesis [18][19][20][21] and tumor suppression [12]. The requirement for parity to induce mammary tumorigenesis in the current model suggests that during pregnancy, lactation and/or involution contextual changes occur that allow full Wnt/ b-catenin pathway activation to occur. Preliminary histopathological analyses (data not shown) have revealed that at mid-lactation, lobulo-alveolar differentiation is incomplete in transgenic glands, and further studies of this process and of involution at both the histological and molecular levels are in progress.
The SNAI2 and SNAI1 genes have recently been identified as direct transcriptional targets of ATF3 in human mammary cells Figure 5. Decreases in gene expression following ATF3 knock-down in EMT6 cells. EMT6 cells were transfected with silencing RNAs A1, A2, or A3 as described in Materials and Methods, or with a scrambled version of A1, at the indicated concentrations. A, 48 h later extracts were analyzed for ATF3 protein expression by immunoblotting (upper panels) or for mRNA expression by qPCR (lower panel). Gene expression levels are expressed relative to expression in cells treated with the scrambled siRNA. The statistical significance of differences between mean expression levels, calculated relative to the scrambled siRNA control, is indicated over the bars of the histograms: *, p,0.05: **, p,0.01: ***, p,0.001. B, qPCR was used to analyze the same extracts for Snai2, Wnt7b, Tcf7, Jun, and Snai1 as indicated. Black bars, scrambled siRNA; dark gray bars, 0.5 nmol A2siRNA; light gray bars, 0.5 nmol A3siRNA; white bars, 1-10 nmol A2siRNA. doi:10.1371/journal.pone.0016515.g005 [19], and the murine homologs, Snai2 and Snai1 are up-regulated in ATF3-induced mammary tumors ( Figure 4). Multiple, bidirectional interactions between Wnt/b-catenin pathway activation and Snail have been identified previously. Snail interacts directly with nuclear b-catenin [52] and indirectly through repression of E-cadherin [49] to increase the transactivation capacity of b-catenin. Wnt pathway activation, on the other hand, up-regulates Snail activity by stabilizing nuclear Snail protein in an Axin2/GSK3b-mediated process [53]. Thus, a direct transcriptional activation of Snail by ATF3 may also be important in activating and/or maintaining Wnt/b-catenin signaling in these tumors through a positive feedback loop.
Global gene expression analyses of human breast tumors have identified an expression pattern consistent with up-regulation of the canonical Wnt/b-catenin pathway that associates with the basal-like tumor subclass [54,55]. Since there is significant overlap between the basal-like subclass and clinically defined triple negative breast cancer [56,57], this implies that Wnt/b-catenin signaling is important in triple negative breast cancer, representing those breast cancers that have the worst prognosis and no effective treatment regimen. Importantly, Rosen's laboratory has recently shown that activation of Wnt/b-catenin signaling is a hallmark of tumor-initiating cells in a mouse model [58]. Thus we have reason to believe that further analysis of the BK5.ATF3 model, in which mammary tumors are clearly basal-like and exhibit activated Wnt/b-catenin signaling, may provide a better understanding of processes that are extremely important in human breast tumorigenesis.

Materials and Methods
Detailed methods for IHC, protein immunoblotting, qPCR, RNA silencing and ChIP analysis are given in Supplementary Materials and Methods (Text S1).

Animals
Mice were maintained in a light and temperature controlled room in an AAALAC-accredited facility, and given water and lab chow ad libitum. All experimental procedures were approved by the Institutional Animal Care and Use Committee of The University of Texas MD Anderson Cancer Center under protocol #05-01-03934. The derivation of the BK5.ATF3 transgenic mice has been described [20]. TOPGAL mice and MMTV.neu mice were obtained from Jackson Laboratory (Bar Harbor, ME). Genotyping was done with appropriate PCR assays, using DNA purified from tail snips.
A tumor experiment with female mice that were hemizygous for both the BK5.ATF3 and TOPGAL transgenes was performed by first allowing the mice to mate and raise pups twice before the age of 6 months. Parous females were palpated twice weekly and were euthanized when palpable tumors reached 1.5 cm in diameter or when animals became moribund. The experiment was terminated at 16 months.

Cell culture
The EMT6 line of murine mammary cancer cells (ATCC: CRL-2755TM) was maintained in Waymouth's MB 752/1 Figure 6. ChIP analysis of ATF3 and JUN binding to selected AP-1 sites. Nuclei from untreated EMT6 cells were prepared for chromatin immunoprecipitation as described in Materials and Methods, and precipitated with antibodies specific for ATF3 or JUN, or with a nonspecific IgG. DNA was purified from the precipitates and used as template in PCR reactions designed to amplify fragments containing AP-1 sites at the indicated distances upstream of the murine genes for: A, Jun; C,D, Tcf7; E, Snai2; F,G, Wnt7b; H, Snai1. In panel B, the PCR targeted a non-specific site over 6000 bp upstream from the Jun transcriptional start site that did not contain an AP-1 site. In each panel, a small fraction of the starting DNA (2%) was used as template for the reaction in the first lane (Input), and the remaining lanes used the indicated immunoprecipitated DNA preparations (IgG, ATF3 and JUN). doi:10.1371/journal.pone.0016515.g006 medium with 2 nM L-glutamine and 15% fetal bovine serum at 5% CO2, and 37uC.

RNA purification and qPCR
Total RNA was isolated using TRIzol reagent (Invitrogen, Grand Island, NY) according to the manufacturer's protocol, and reverse transcribed using the High Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA). Real-time PCR was performed with an ABI Prism 7900HT Sequence Detection System (Applied Biosystems). Student's t-test (two-tailed, assuming unequal variance) was used to determine statistical significance (pvalues) for differences in the relative expression values between tissues for each gene analyzed. p-values less than 0.05 were considered significant.

Supporting Information
Text S1 Supplementary Materials and Methods (DOC) Figure   S1 Mammary tumorigenesis in parous BK5.ATF3; TOPGAL mice. Singly transgenic animals from the BK5.ATF3 line and the TOPGAL line were mated to produce double heterozygotes (ATF3 +/2 , TOPGAL +/2 ). The doubly heterozygous females and their singly transgenic (ATF3 2/2 , TOPGAL +/2 ) littermates were allowed to mate and raise litters twice, and then monitored for mammary tumor formation until 16 months of age. Tumor-bearing animals were sacrificed when a tumor reached 1.5 cm in its longest dimension. A. Survival curves are shown for several different genotypes. Data for TOPGAL non-TG/ATF3 TG mice is from reference (Wang et al., 2008). B. Tumors arising in (ATF3 +/2 , TOPGAL +/2 ) females were harvested and analyzed by histopathology. Scale bar in panel B = 50 mm. (TIF)