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Phase-locked 10 MHz reference signal for frequency domain time-resolved fluorescence measurements.

A complete electronic system that is suitable for use in megahertz frequency domain time-resolved fluorescence instruments based on mode-locked lasers is described. The circuit produces a 10 MHz signal, phase locked to the mode-locked laser pulse frequency, which is required by many commercial frequency synthesizers as the external reference signal. This device is particularly useful in conjunction with ultrafast gated intensified charge coupled device cameras capable of being frequency modulated for time-resolved fluorescence imaging.