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Structural and Biochemical Basis for the Binding Selectivity of Peroxisome Proliferator-activated Receptor γ to PGC-1α*

The functional interaction between the peroxisome proliferator-activated receptor γ (PPARγ) and its coactivator PGC-1α is crucial for the normal physiology of PPARγ and its pharmacological response to antidiabetic treatment with rosiglitazone. Here we report the crystal structure of the PPARγ ligand-binding domain bound to rosiglitazone and to a large PGC-1α fragment that contains two LXXLL-related motifs. The structure reveals critical contacts mediated through the first LXXLL motif of PGC-1α and the PPARγ coactivator binding site. Through a combination of biochemical and structural studies, we demonstrate that the first LXXLL motif is the most potent among all nuclear receptor coactivator motifs tested, and only this motif of the two LXXLL-related motifs in PGC-1α is capable of binding to PPARγ. Our studies reveal that the strong interaction of PGC-1α and PPARγ is mediated through both hydrophobic and specific polar interactions. Mutations within the context of the full-length PGC-1α indicate that the first PGC-1α motif is necessary and sufficient for PGC-1α to coactivate PPARγ in the presence or absence of rosiglitazone. These results provide a molecular basis for specific recruitment and functional interplay between PPARγ and PGC-1α in glucose homeostasis and adipocyte differentiation.

Peroxisome proliferator-activated receptor ␥ (PPAR␥) 3 and its two related receptors, PPAR␣ and ␤/␦, comprise a subfamily of ligand-regulated nuclear receptors involved in many aspects of human physiology. PPAR␥ is a key regulator involved in adi-pocyte differentiation, glucose homeostasis, and inflammatory responses (1,2). PPAR␥ also is the molecular target of pioglitazone (Actos) and rosiglitazone (Avandia), a class of thiazolidinedione drugs used for treating type 2 diabetes patients. These drugs improve insulin sensitivity and increase glucose and fatty acid metabolism. PPAR␥ ligands may also have application in the treatment of inflammation and cancer (3,4). However, the clinical use of PPAR␥ ligands is clearly tempered by side effects such as edema, weight gain, and increased incidence of heart attack. The undesired side effects of thiazolidinedione drugs are possibly associated with the cross-reactivity of these ligands with two other PPARs because of low selectivity and/or the inherent PPAR␥ activation because of multiple functions of PPAR␥ (5,6).
The pharmacological actions of rosiglitazone are mediated through the PPAR ligand-binding domain (LBD), which binds ligands and then recruits nuclear receptor coactivators (or corepressors) to regulate the expression of downstream target genes. Currently there are ϳ300 nuclear receptor coregulators, including the steroid receptor coactivators (SRC) 1, 2, and 3 and the nuclear corepressors N-CoR and SMRT (7,8). The functional profile of PPAR␥ in response to ligand binding is largely determined by the selective use of transcriptional coregulators because ligand-specific recruitment of coregulators ultimately controls the transcriptional output of the target genes. Thus, ligand-bound PPAR␥ may show diverse pharmacological functions depending on the specific binding of coactivators. Analogous to many other nuclear receptors, the ligand-dependent recruitment of coactivators by PPAR␥ is primarily determined by the interaction of coactivator LXXLL motifs with the receptor LBD. Crystal structures of various LBD⅐coactivator complexes reveal a conserved binding mode for coactivator LXXLL motifs by nuclear receptors (9,10). Upon the binding of an agonist, nuclear receptors use a charge-clamp pocket, in part composed of the C-terminal activation function 2 helix, to form a hydrophobic groove for binding the LXXLL motif of the coactivators. However, there are numerous coactivators with distinct functions, each containing multiple LXXLL motifs. The precise mechanism for recruitment of specific coactivators by PPAR␥ remains poorly defined.
The PPAR␥ coactivator 1␣ (PGC-1␣) is a nuclear coactivator that interacts with many transcription factors, including most members of the nuclear hormone receptor family. PGC-1␣ was originally identified as a coactivator that interacts with PPAR␥ in a ligand-independent manner (11). Besides PPAR␥, PGC-1␣ is also able to coactivate a number of other nuclear receptors through conserved LXXLL motifs, including glucocorticoid receptor, PPAR␣, estrogen receptor, and estrogen-related receptor ␣ (ERR␣) (12)(13)(14)(15)(16). PGC-1␣ is expressed in brown adipose tissue, brain, heart, kidney, and cold-exposed skeletal muscle (17). In white adipose tissue, the expression of PGC-1␣ is substantially increased by treatment with the thiazolidinedione rosiglitazone, suggesting the importance of PGC-1␣ in rosiglitazone-regulated PPAR␥ activity (18). Mapping of PGC-1␣ defined one nuclear receptor interaction domain with a consensus LXXLL motif (residues 144 -148, ID1) and an LLKYL motif (residues 210 -214, ID2) that are important for binding nuclear receptors. For example, both biochemical data and the crystal structure of the ERR␣ LBD bound to the PGC-1␣ LLKYL motif (ID2) reveal the specific binding of this inverted leucine-rich motif to ERR␣ (15,19). However, the molecular basis for the interaction between PPAR␥ and PGC-1␣ remains unclear.
To investigate the molecular mechanisms of rosiglitazoneregulated PPAR␥ activity, we first determined the preferred coactivator motifs for PPAR␥ in response to rosiglitazone using biochemical peptide profiling. Among the coactivator motifs tested, the PGC-1␣ ID1 motif was shown to have the highest binding affinity to PPAR␥. To uncover the molecular mechanism for the binding selectivity of PPAR␥ to PGC-1␣, we went on to solve the crystal structure of the PPAR␥ LBD bound to a large fragment of PGC-1␣ containing both ID1 and ID2 LXXLL-related motifs. The structure revealed that only ID1 is capable of binding to PPAR␥. Mutations within the context of the full-length PGC-1␣ indicate that the first PGC-1␣ motif is necessary and sufficient for PGC-1␣ to coactivate PPAR␥ in the presence or absence of rosiglitazone. These results provide a structural and biochemical basis for specific recruitment of PGC-1␣ by the rosiglitazone-bound PPAR␥.

EXPERIMENTAL PROCEDURES
Protein Preparation-Both the human PPAR␥ LBD (residues 206 -477) and the human PGC-1␣ fragment (residues 101-220), each containing a His 6 tag, were expressed from the expression vector pETDuet1 (Novagen). BL21(DE3) cells transformed with this expression plasmid were grown in LB broth at 25°C to an A 600 of 1 and induced with 0.1 mM isopropyl 1-thio-␤-D-galactopyranoside at 16°C. Cells were harvested, resuspended in 400 ml of extract buffer (50 mM Tris, pH 8.0, 150 mM NaCl, 10% glycerol, and 25 mM imidazole) per 12 liters of cells, and passed three times through a French press with pressure set at 1000 pascals. The lysate was centrifuged at 20,000 rpm for 30 min, and the supernatant was loaded onto a nickel-nitrilotriacetic acid column. The column was washed with extract buffer, and the protein was eluted with a 300-ml gradient to the buffer (10 mM Tris, pH 8.0, 150 mM NaCl, 10% glycerol, and 500 mM imidazole). Both human PPAR␥ LBD and the PGC-1␣ fragment were further purified on a Q-Sepharose column. The purified PPAR␥ and the PGC-1␣ fragment that contains both LXXLL motifs were mixed at a ratio of 1:2 with a 5-fold excess of the PPAR␥ ligand rosiglitazone. The ternary complex was further purified by gel filtration (20 mM Tris, pH 8.0, 100 mM NaCl, and 5 mM DTT) and filter-concentrated to 10 mg/ml. Crystallization, Data Collection, Structure Determination, and Refinement-The PPAR␥⅐rosiglitazone⅐PGC-1␣ crystals were grown at room temperature in hanging drops containing 1.0 l of the above protein solution and 1.0 l of well solution containing 0.2 M ammonium iodide (pH 6.2) and 20% polyethylene glycol 3350. The crystals were directly frozen in liquid nitrogen for data collection. The PPAR␥⅐rosiglitazone⅐PGC-1␣ crystals formed in the C2 space group, with a ϭ 95.11, b ϭ 53.89, and c ϭ 64.89 Å, ␣ ϭ ␤ ϭ 90°, and ␥ ϭ 105°, and contained one molecule/crystallographic asymmetric unit. A full 360°of data were collected from a single crystal using 1°oscillation by a MAR165 CCD detector at the ID line of sector 32 of the Advanced Photon Source. The observed reflections were reduced, merged, and scaled with the HKL2000 package (20). The structures were determined with the AmoRe program by molecular replacement using the crystal structure of PPAR␥ LBD (21) as a model (22). Manual model building was carried out with QUANTA (Accelrys, Inc.), and structure refinement was proceeded with crystallography NMR software (23).
AlphaScreen Binding Assays-The binding of various peptide motifs to PPAR␥ was determined by AlphaScreen assays using a hexahistidine detection kit from PerkinElmer Life Sciences, as described recently for other nuclear receptors (24,25). The experiments were conducted with ϳ20 nM His tag receptor LBD and 20 nM biotinylated SRC2-3 peptide or other coactivator peptides in the presence of 5 g/ml donor and acceptor beads in a buffer containing 50 nM MOPS, 50 mM NaF, 50 mM CHAPS, and 0.1 mg/ml bovine serum albumin, all adjusted to pH 7.4. The biotinylated peptides used in Fig The relative binding affinity of peptide LXXLL motifs was determined using unlabeled peptides at 500 nM to compete with the binding of biotinylated SRC2-3 to PPAR␥ LBD. The sequences of unlabeled peptides used in Fig. 1B are as before (26). IC 50 values for various coactivator LXXLL motifs were determined from a nonlinear least squares fit of the data based on an average of three repeated experiments from the doseresponse curves.

PGC-1␣ LXXLL Motif Binding Affinity for PPAR␥ in Response
to the Ligand Rosiglitazone-The recruitment of specific coactivators by PPAR␥ is the major factor that determines the transcriptional profile of target genes and the pharmacological actions of rosiglitazone (27,28). To characterize the binding properties of various coactivators in response to rosiglitazone, we measured the direct interactions of purified PPAR␥ LBD with a panel of biotinylated peptide motifs from coactivators using AlphaScreen assays (26,29). In this assay, the coactivator peptides and the PPAR␥ LBD protein were attached to donor and acceptor beads, respectively. Upon interaction between the coactivator peptides and the PPAR␥ LBD, excitation with a laser beam at 680 nm causes the donor beads to emit single oxygen molecules that activate fluorophores in the acceptor beads, and the light is recorded at 520 -620 nm. As shown in Fig. 1A, the rosiglitazone-bound PPAR␥ LBD induced a strong interaction with various coactivator LXXLL motifs from the SRC family of coactivators, CBP, TRAP220, and the PGC-1␣ ID1 motif. These data are in agreement with the agonist property of rosiglitazone. Notably, even in the absence of rosiglitazone, PPAR␥ showed significant interactions with several coactivator motifs (particularly the PGC-1␣ ID1 motif), consistent with the high basal activation properties of PPAR␥.
To determine which coactivators are preferentially recruited, we performed a peptide profiling experiment using a panel of unlabeled peptides to compete off the binding of the biotinylated third LXXLL motif of SRC2 (SRC2-3) to the rosiglitazone-bound PPAR␥ LBD (Fig.  1B). In this experiment, all unlabeled peptides were applied at a uniform concentration of 500 nM under identical experimental conditions. Thus the relative binding affinity of individual peptides to PPAR␥ LBD can be measured by the degree of its inhibition of the binding of the biotinylated SRC2-3 motif to the PPAR␥ LBD. In the absence of any competing peptides, interaction between the biotinylated SRC2-3 motif and the PPAR␥ LBD yielded a count of 27,000 photons (Fig. 1B). Most unlabeled peptide motifs gave little competition to coactivator binding of PPAR␥ at a concentration of 500 nM; the PGC-1␣ ID1 motif showed the most significant competition.
The high binding affinity of PPAR␥ toward the PGC-1␣ ID1 motif was further determined by IC 50 values from quantitative competition experiments using unlabeled peptides (Fig. 2). Consistent with the peptide profiling, PPAR␥ bound to the PGC-1␣ ID1 motif with higher affinity than to ID2 (IC 50 of 1.7 M versus 87 M) ( Fig. 2A). Relative to the IC 50 of LXXLL motifs from SRC families, the PGC-1␣ ID1 motif also displayed stronger binding affinity to PPAR␥ (Fig. 2B). Interestingly, rosiglitazone increased the binding affinity of PPAR␥ with most coactivator motifs tested. The remarkable selectivity of PPAR␥ toward PGC-1␣ is consistent with the fact that PGC-1␣ is a well established coactivator for PPAR␥ in adipogenesis and glucose metabolism (30). Together, these results demonstrate that rosiglitazone promotes the interaction of coactivator motifs with PPAR␥ and that PGC-1␣ ID1 preferentially binds to PPAR␥ with the highest affinity among all coregulator motifs tested.
Structure of the PPAR␥ LBD⅐Rosiglitazone⅐PGC-1␣ Complex-Because the first LXXLL motif of PGC-1␣ (ID1) binds to PPAR␥ with the highest affinity, we wanted to know the molecular mechanism underlying the binding selectivity of PPAR␥ toward PGC-1␣. To determine the roles of the two receptorinteracting motifs (ID1 and ID2) of PGC-1␣ in binding to PPAR␥, we purified a PGC-1␣ fragment (residues 101-220) containing both ID1 and ID2 interacting motifs and complexed it with the PPAR␥ LBD for crystallization studies (Fig. 3A). The PPAR␥ LBD and the PGC-1␣ fragment were purified individually, mixed to form the complex with PGC-1␣ in excess, and then further purified through a gel filtration column (Fig. 3, B and C). In the gel filtration profile, the PGC-1␣⅐PPAR␥ complex is shifted left because of its greater size, whereas PGC-1␣ alone eluted out in the volume of smaller size. We collected and concentrated the complex peak to a final concentration of 10 mg/ml for crystallization trials, and PPAR␥⅐rosiglitazone⅐PGC-1␣ crystals were readily obtained at room temperature (Fig. 3D).
The PPAR␥⅐rosiglitazone⅐PGC-1␣ complex crystallized in the C2 space group with one complex in each asymmetric unit. The structure was solved by molecular replacement using the structure of the PPAR␥ LBD bound to rosiglitazone and the SRC1-2 motif as the initial model (21). The calculated electron density map using the initial molecular replacement solution showed clear features of the PPAR␥ LBD, the bound ligand rosiglitazone, and a single PGC-1␣ LXXLL motif. Although a large PGC-1␣ fragment (120 amino acids) was used in crystallization, most sequences except the ID1 motif are invisible because of very low and ambiguous electron density. The statistics of data and the refined structure are listed in Table 1.
The overall structure of the PPAR␥⅐rosiglitazone⅐PGC-1␣ complex is shown in Fig. 4A. Specifically, the PPAR␥ LBD is composed of 13 ␣ helices and four short ␤ strands that are folded into a three-layer helical sandwich. The C-terminal activation function 2 helix is positioned in the active conformation by packing tightly against the main domain of the LBD. In this conformation, the activation function 2 helix, together with helices H3, H4, and H5, forms a charge-clamp pocket where the PGC-1␣ LXXLL motif is docked.
Functional Interaction of PGC-1␣ and PPAR␥ through the PGC-1␣ ID1 Motif-Despite both the ID1 and ID2 motifs being in the PGC-1␣ fragment used for crystallization, the structure of the PPAR␥ LBD⅐PGC-1␣ complex shows that only the ID1 motif is bound in the coactivator binding site. The LKKLL sequence of the PGC-1␣ ID1 motif can be clearly identified by the distinguishing features of the electron density map, where the core LKKLL motif adopts a two-turn ␣ helix with its hydrophobic leucine side chains directed toward the hydrophobic surface of the coactivator binding groove of PPAR␥ (Fig. 4, B and C). The overall structure of the PPAR␥ coactivator binding site and the docking mode of PGC-1␣ ID1 resemble those observed in other nuclear receptor coactivator complexes (9). Beside the core LKKLL motifs, several flanking residues are also clearly visible in the electron density map (Fig. 4B). Thus, the structure of the PPAR␥ LBD⅐PGC-1␣ complex and the biochemical Alpha-Screen results both indicate that the binding of PGC-1␣ to PPAR␥ is through the PGC-1␣ ID1 LXXLL motif.
To further establish the role of PGC-1␣ ID1 and ID2 in binding to PPAR␥, we performed mammalian two-hybrid assays using the PGC-1␣ fragment that contains both these motifs. The PGC-1␣ fragment was fused with the Gal4 DNA-binding domain, and the PPAR␥ LBD was fused with the VP16 activation domain (Fig. 4, D and E). We mutated individual LXXLL motifs of PGC-1␣ to AXXLA, resulting in PGC-1␣M1 and PGC-1␣M2, and tested the ability of these mutated coactivators to potentiate the PPAR␥-mediated activation in response to rosiglitazone. Mutations in the ID2 motif of PGC-1␣ decreased the interaction with PPAR␥, whereas the activation in response to rosiglitazone remained unchanged (Fig. 4D). However, mutation of the ID1 motif completely abolished the ability of PGC-1␣ to bind PPAR␥, consistent with the dominant role of ID1 in binding to PPAR␥ as revealed by the structure and the AlphaScreen assays.
PGC-1␣ is also able to coactivate a number of other nuclear receptors, including ERR␣, a constitutively active receptor. To compare PGC-1␣ interactions between PPAR␥ and ERR␣, we performed similar mammalian two-hybrid experiments on PGC-1␣ and ERR␣. As expected, ERR␣ interacts with PGC-1␣ in the absence of any ligand treatment, consistent with the high constitutive activity of ERR␣ (Fig.  4E). In contrast to PPAR␥, mutations in the ID1 or ID2 motifs of PGC-1␣ both substantially reduced ERR␣-PGC-1␣ interactions. These results suggest that PGC-1␣ uses different interacting domains (ID1 or ID2) in its preferential binding to various nuclear receptors: PPAR␥ prefers ID1, and ERR␣ requires both ID1 and ID2.
To further determine the functional significance of PGC-1␣ ID1 in the ligand-regulated activity of PPAR␥, we mutated either the ID1 or ID2 motifs of PGC-1␣ within the context of the fulllength PGC-1␣ coactivator and tested the effect of these mutated coactivators on PPAR␥-mediated activation in cellbased assays (Fig. 5). The plasmid encoding full-length PPAR␥ and a PPAR␥ response reporter were transiently cotransfected into cells, and the assays were performed in the presence or absence of rosiglitazone. Fig. 5 shows that wild-type PGC-1␣ substantially elevates the transcriptional activity of PPAR␥ with or without rosiglitazone. Although no effect on reporter activity was observed upon cotransfection with the PGC-1␣ ID2 mutant, the mutation in the ID1 motif completely abolished the ability of PGC-1␣ to coactivate PPAR␥-mediated transcription. These results further demonstrate the functional significance of the PGC-1␣ ID1 in binding to PPAR␥ and highlight a crucial role of the PGC-1␣ ID1 motif in the coactivation of PPAR␥.
Molecular Determinants for the Preferential Recruitment of PGC-1␣ by PPAR␥-The PPAR␥ LBD⅐PGC-1␣ structure is the first structure of a nuclear receptor with the PGC-1␣ ID1 motif, and it reveals a molecular basis for the preferential binding of this motif to the PPAR␥ receptor. In addition to the hydrophobic interactions with PPAR␥ by its core LXXLL motif, which is conserved for the binding of nuclear receptors (Fig. 6A),  PGC-1␣ contains two unique features within the structure that define its high affinity binding to PPAR␥ (Fig. 6, B-D). The first feature is that Lys 145 in the core region of PGC-1␣ ID1 forms a direct hydrogen bond with Asn 312 in PPAR␥ (Fig. 6, B and C). This hydrogen bond stabilizes the binding of PPAR␥ and PGC-1␣ in addition to the hydrophobic interactions between these two molecules. The second feature is the remarkable stability of the PGC-1␣ ID1 helix through its intramolecular interactions. In the structure, Ser 142 forms a direct hydrogen bond that caps the backbone amide of Glu 140 of the LXXLL helix (Fig. 6D). These intramolecular interactions are likely to stabilize the overall helical structure of the PGC-1␣ ID1 motif, thus facilitating the hydrophobic docking of this helix into PPAR␥. Together, these unique intermolecular and intramolecular contacts serve as a basis for the high affinity and specific binding of PPAR␥ toward PGC-1␣.
To assess the importance of these interactions in the PGC-1␣-mediated enhancement of PPAR␥ activity, we made the mutations S142A and K145A in PGC-1␣ ID1 and tested these mutants in cell-based assays using full-length PPAR␥ and a PPAR␥ response reporter (Fig. 5). In the absence of ligand, the S142A mutation substantially reduced PGC-1␣-mediated coactivation of PPAR␥. PGC-1␣-mediated coactivation was also impaired by this mutation in the presence of rosiglitazone. Similar results were also observed for the K145A mutant, suggesting that both Ser 142 and Lys 145 of PGC-1␣ contribute to its ability to coactivate PPAR␥. Together, these results reveal that the strong interaction of PGC-1␣ with PPAR␥ is due to hydrophobic binding of its ID1 LXXLL motif and also to specific interactions between the two molecules.

DISCUSSION
As a master regulator of adipose differentiation, PPAR␥ serves as a key transcriptional factor that links obesity, diabetes, and cardiovascular diseases through selective recruitment of various coregulatory proteins with distinct functions. The various transcriptional coactivators display many distinct physiological roles, such as enzymatic activities like histone acetylation and deacetylation. For instance, SRC1 and SRC2 (also known as GRIP1/TIF2) have been shown to play opposite roles C, an alignment of core sequences of PGC-1␣ ID1 and ID2. D and E, the roles of PGC-1␣ ID1 and ID2 in the interaction with PPAR␥ and ERR␣. Mammalian two-hybrid assays were performed in COS-7 cells. The PGC-1␣ fragment that contains both ID1 and ID2 was fused to the Gal4 DNA-binding domain, and the LBDs of ERR␣ and PPAR␥ were fused with VP16 AD. The ligand-dependent interaction of PPAR␥ with PGC-1␣ was analyzed using 1 M rosiglitazone (Rosi).

Basis for the Binding of PGC-1␣ to PPAR␥
in energy metabolism in mouse knock-out studies (31). SRC1 Ϫ/Ϫ mice are prone to obesity because of reduced energy expenditure, whereas SRC2 Ϫ/Ϫ mice are protected against obesity and display enhanced adaptive thermogenesis. Accordingly, N-(9-fluorenyl)methoxycarbonyl (Fmoc)-L-leucine, a distinct PPAR␥ ligand, preferentially recruits SRC1 (but not SRC2) by inducing a particular allosteric configuration of the PPAR␥ coregulator binding interface (32). As a result, this unique ligand enhances insulin sensitivity and reduces glucose levels without promoting the weight gain normally associated with the thiazolidinedione class of PPAR␥ ligands. Thus, the selective modulation of PPAR␥ activity is highly dependent on its ability to recruit specific coactivators.
Here we conducted detailed analysis of the interplay between rosiglitazone-regulated PPAR␥ and coactivators through a combination of mutagenesis, biochemical binding profiling, and structural analysis. Our quantitative competition experiments show that rosiglitazone-bound PPAR␥ has a marked binding preference for the PGC-1␣ ID1 motif (Figs. 1 and 2). Consistent with this result, the crystal structure of the PPAR␥⅐PGC-1␣ complex reveals the docking mode of the PGC-1␣ ID1 helix (Fig. 4, A and B). Our mutagenesis studies further demonstrate the essential roles of the ID1 motif in the functional interaction between PGC-1␣ and PPAR␥ (Figs. 4D and 5). Interestingly, increased expression of PGC-1␣ has been shown in rosiglitazone treatment (18,27). The coexpression of PGC-1␣ and PPAR␥ in rosiglitazone target tissues and the strong interaction between them suggest the importance of the interaction of PPAR␥ and PGC-1␣ in the pharmacological roles of rosiglitazone.
The molecular basis for the selective binding of PPAR␥ with PGC-1␣ is provided by the structure of the bound pair. This structure reveals specific intermolecular and intramolecular interactions that define the preferential binding between rosiglitazone-bound PPAR␥ and PGC-1␣ (Fig. 6). Mutations designed to disrupt these interactions reduce the binding of PGC-1␣ to PPAR␥ in cellbased transcriptional assays (Fig. 5).
In addition, we have demonstrated that different receptors can differ in their interaction with PGC-1␣ by using alternative interaction sites. Although ID2 is not required for PGC-1␣ interaction with PPAR␥, this motif is shown to bind to the nuclear receptor ERR␣ (15,19). Interestingly, ID2 contains an atypical LXXYL motif, which is an inverted LXXLL sequence. Instead of three hydrophobic leucine side chains, ID2 uses two leucine side chains to dock into the groove of the ERR␣ coactivator binding site. The interaction is further strengthened by the favorable van der Waals contacts between the tyrosine in the PGC-1␣ ID2 core and ERR␣ residues Leu 333 , Ile 336 , and Leu 509 (19). However, these ERR␣ residues are not conserved in PPAR␥, and the coregulator binding interface of PPAR␥ shows binding properties distinct from those of ERR␣. As such, only PGC-1␣ ID1 is capable of strong binding to PPAR␥, whereas PGC-1␣ ID2 has a much lower affinity. Structural comparison between the PPAR␥⅐PGC-1␣ ID1 and ERR␣⅐PGC-1␣ ID2 complexes shows that these two nuclear receptors employ distinct structural mechanisms to achieve specific recognition of their coactivators. As PGC-1␣ also strongly interacts with PPAR␣, PPAR␤/␦, and a large number of other nuclear receptors, the structure of the PPAR␥⅐PGC-1␣ complex will have broad implications for understanding PGC-1␣ receptor interactions.