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Genetic Analysis of Immunity to Soybean Cyst Nematode Race 3 in Elite Line Zhongpin 03-5373

Soybean cyst nematode(SCN) race 3, one of the eight races identified in China, is widely distributed and severely reduced soybean yield. Zhongpin 03-5373(ZP03-5373) is an elite line immune to SCN race 3. In this study, a recombinant inbred line(RIL) population was developed from a cross between ZP03-5373 and Zhonghuang 13(ZH13). A genetic linkage map was constructed using a total of 506 molecular markers, including SSRs, EST-SSRs, In Del, and SNP. The total length of the genetic map was 2651.90 c M with an average marker spacing of 5.24 c M. Based on the phenotyping data, we detected three QTL intervals to dominate SCN3, including SCN3-7(Gm07), SCN3-11(Gm11), and SCN3-18(Gm11). Main effect QTL, SCN3-18 could explain 29.5% of resistant variation. Two minor effect QTLs, SCN3-7 and SCN3-11, explained 6.2% and 5.5% of resistant variation, respectively. And it further showed there was significant epistatic interaction between SCN3-7 and SCN3-18 for resistance to SCN3. Both SCN3-7 and SCN3-11 were confirmed to be resistant to SCN3 by tracking flanking marker in the ancestors of ZP03-5373. These markers will be helpful for developing SCN resistant cultivars and cloning resistant genes by marker assisted selection.