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The Rate of Clonal Transformation of B‐Lymphocytes by Epstein‐Barr Virus in Childhood

The rate of clonal transformation of the B‐lymphocyte by Epstein‐Barr virus (EBV) in children was studied in semisolid agar culture. A remarkable influence of the cell donor's age on the rate was found: In the order of age, the rate was highest in cord blood, lower in infants, lowest in young children, somewhat higher in school children and high in adults. The rate of EBV‐associated nuclear antigen (EBNA)‐positive cells 24 h after infection showed little age‐dependent difference. The only significant difference was found between cord blood and young children. From these results, the age‐dependent difference in the rate of clonal transformation seemed to be mainly in the process after EBNA expression through transformed cell colony formation.