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Holographic mensuration of suspended particles in aquatic systems

The distribution and dynamics of aggregates in the aquatic environment play an important role in the modeling of biogeochemical processes. Previous work on aggregates in the ocean (e.g. sedimentary 'marine snow' particles), which vary in size from tens of microns to several millimeters, has used electronic counting or conventional photogrpahy coupled with image analysis. Here we describe a nondestructive in situ approach by use of holographic mensuration, hologrammetry, that affords greater scope and higher accuracy for the enumeration, sizing, and spatial distribution determination of aggregate particles. By means of two complimentary techniques, in-line and off-axis transmission holography, we present the initial experiments conducted in our laboratory and discuss the preliminary results from real image analysis.