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[The culture of retinal muller cells and expression of its glutamate transporter GLAST].

PURPOSE
To culture retinal Müller cells (RMC) of infantile and mice in postnatal day 3 (P3) and study the expression of GLAST.


METHODS
RMCs of infantile and mice in P3 were cultured by tissue inoculation and shaken to allow detachment of other retinal cells. Then the morphologic and growth characteristics of the cells was oberseved every day. The second passage cells were identified with GFAP, Vimentin and the expression of GLAST was observed with immunofluorescent staining.


RESULT
The cultured RMCs reached confluence in 15-20 days. These cells were positive in GFAP, Vimentin and GLAST antibody staining.


CONCLUSIONS
RMCs of infantile and mice in P3 can be successfully cultured by tissue inoculation, GLAST widely expresses on RMCs membrane.