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Influence of selenium on the mycelia of the shaggy bracket fungus, Inonotus hispidus.

BACKGROUND
Selenium is a needed trace element for animals and humans. Many fungi have effective mechanisms to acquire, transform and accumulate selenium (Se) in organic forms. In this study, the effects of inorganic Se (sodium selenite) on the medicinal fungus, Inonotus hispidus was investigated.


RESULTS
I. hispidus was capable of tolerating up to 3.85 mM selenite, at which ~85% growth inhibition was seen, with 50% growth inhibition occurring at ~1 mM selenite. Growth in 0.29 mM Se resulted in I. hispidus mycelium with 115 times higher Se-levels compared to growth in standard media, and an organic Se content of 86% to total Se content. The influence of Se accumulation on morphological features of I. hispidus were examined by microscopic and SEM observation. These data revealed significant shrinkage and deformations of I. hispidus hyphae with decreased branching and collapse of clamp-connections under higher Se stress. However, conidial production in I. hispidus increased dramatically. The influence of Se on the mycelial growth could be recovered by re-inoculation in standard media. Se accumulation had only minimal impacts on the yield of the potential selenocompounds such as amino acids, proteins and polysaccharides. By contrast, Se-enriched I. hispidus mycelium were of higher quality due to reduction in crude fat and total ash contents.


CONCLUSIONS
These data provide basic and applied information on the feasibility of producing selenized I. hispidus as an enriched and better quality product. This article is protected by copyright. All rights reserved.