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Light Scatter Characteristics of Erythroid Precursor Cells Studied in Flow Analysis

Summary Light scatter (LS) differences among cells of rabbit marrow was studied by flow analysis using a Coulter two-parameter cell sorter. A preliminary fractionation of the marrow into samples enriched with cells of varying degrees of maturation was accomplished in Ficoll/Hypaque density gradients. Subsequent study of each of these cell samples in flow analysis demonstrated unique LS profiles which distinguished erythrocytes from nucleated erythroid precursors and granulocyte precursors. The combined separation procedures made it possible to sort fractions of erythroid precursors with as high as 90% erythroid cells, two-thirds of which were nucleated precursors.