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Determination of the Podophyllotoxin Ointment and Its Related Substances by R-HPLC

Objective:A R-HPLC method was established for the determination of Podophyllotoxin Ointment and its related substances. Methods:The separation was performed on a C18 column(5μm,4.6mm×200mm). The mobile phase was composed of methanol∶water=55∶45. The flow rate was 1.0mL·min-1. The detection was carried out with a UV detector at292 nm. Results:The calibration curves were linear between 0.303~0.707mg/mL(r=0.9998). Three tested solution of differ concentration RSD were 1.05%(n=5),0.87%(n=5) and 1.16%(n=5). Conclusion:The method is simple,rapid,accurate and is suitable for the determination of Podophyllotoxin for the determination of Podophyllotoxin Ointment and its related substances.