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Synthesis of 1 , 3 #-Glucanase and P-N-Acetylglucosaminidase during Autolysis of Neurospora crassa

The synthesis of 1,3-P-glucanase, P-N-acetylglucosaminidase and total protein was studied during autolysis of Neurospora crassa. Evidence for de novo protein synthesis during autolysis was obtained from the incorporation of [3H]tryptophan and [3H]phenylalanine into the protein pool and from the inhibitory effects of cycloheximide and puromycin. Glucose accumulated in the growth medium during autoly sis and N-acetylglucosamine and two unidentified oligosaccharides were detected towards the end of the 46 d autolysis period. During autolysis, 1,3-P-glucanase accumulated in the medium to tenfold greater activities than P-N-acetylglucosaminidase. The former enzyme had a pH optimum of 5 . 5 and a K , of 0.45 mM. It was inhibited by excess substrate but not by excess glucose.