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Optical Microscope Automatic Focusing Methods Research

This paper proposes an auto-focus method that is based on Ostu segment. The method uses the variance of the two classes of the segment results as the guidance of the auto-focus system. This guidance function responses to wide range of object distance range, it has a strong anti jamming ability and it provides a wide range of the guidance value. The result shows that this method can work well and the time it takes to focus is unconcerned to the position when the focus process starts and it responses to a wide range of object distance.