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Short-term repeated corticosterone administration enhances glutamatergic but not GABAergic transmission in the rat motor cortex

It has been demonstrated that stress impairs performance of skilled reaching and walking tasks in rats due to the action of glucocorticoids involved in the stress response. Skilled reaching and walking are controlled by the primary motor cortex (M1); however, it is not known whether stress-related impairments in skilled motor tasks are related to functional and/or structural alterations within the M1. We studied the effects of single and repeated injections of corticosterone (twice daily for 7 days) on spontaneous excitatory and inhibitory postsynaptic currents (sEPSCs and sIPSCs) recorded from layer II/III pyramidal neurons in ex vivo slices of the M1, prepared 2 days after the last administration of the hormone. We also measured the density of dendritic spines on pyramidal cells and the protein levels of selected subunits of AMPA, NMDA, and GABAA receptors after repeated corticosterone administration. Repeatedly administered corticosterone induced an increase in the frequency but not in the amplitude of sEPSCs, while a single administration had no effect on the recorded excitatory currents. The frequency and amplitude of sIPSCs as well as the excitability of pyramidal cells were changed neither after single nor after repeated corticosterone administration. Treatment with corticosterone for 7 days did not modify the density of dendritic spines on pyramidal neurons. Corticosterone influenced neither the protein levels of GluA1, GluA2, GluN1, GluN2A, and GluN2B subunits of glutamate receptors nor those of α1, β2, and γ2 subunits of the GABAA receptor. The increase in sEPSCs frequency induced by repeated corticosterone administration faded out within 7 days. These data indicate that prolonged administration of exogenous corticosterone selectively and reversibly enhances glutamatergic, but not GABAergic transmission in the rat motor cortex. Our results suggest that corticosterone treatment results in an enhancement of spontaneous glutamate release from presynaptic terminals in the M1 and thereby uncovers a potential mechanism underlying stress-induced motor functions impairment.


Introduction
In the rat brain, the primary motor cortex (M1) contains motor maps, which are representations of how movements are organized [8,32]. Examples of M1-controlled motor functions in the rat include skilled limb use in reaching for food [49] and in rung ladder walking [1]. Motor skill learning is associated with a reorganization of movement representations within the rat M1 [19,36]. The mechanism of motor skill learningrelated plasticity of the M1 involves activity-dependent, longterm strengthening of excitatory synaptic connections within the cortical layer II/III [37][38][39]. Motor skill learning has also been reported to induce an increase in synapse number in the M1 [20]. It has been shown that acute and chronic stress impairs the movement accuracy in skilled reaching and walking tasks and alters skilled movement patterns in the reaching task [21,29]. Stress-related impairments in skilled motor tasks may be related to functional and/or structural plasticity of excitatory synapses within rat M1; however, this possibility has not yet been investigated.
While the effects of acute stress on the brain are usually transient, chronic stress is thought to exert a persistent, detrimental influence, which is generally attributed to a prolonged hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis and an elevated level of corticosteroids acting at the cellular level through glucocorticoid and mineralocorticoid receptors (GRs and MRs, respectively; reviewed in [5,25,33]). Repeated stress has been reported to induce suppression of glutamatergic transmission and a decrease in the expression level of glutamate receptors and synaptic proteins in the rat medial prefrontal cortex (mPFC), which is an important target for glucocorticoids involved in the stress response [24,51]. Moreover, repeated stress results in profound morphological changes of pyramidal neurons in the mPFC (reviewed in [41]). GR and MR receptors are expressed in the rat M1 [30,40], and it has been reported that MR and GR blockade ameliorates motor impairments resulting from stress [15]. However, the effects of stress on the structure and function of the rat motor cortex remain largely unknown.
Since 21 days of repeated corticosterone injections reliably increase depression-like behavior [11], repeated corticosterone administration has been proposed as a preclinical rat model of chronic stress (reviewed in [42]). One advantage of this model is that it directly examines the influence of an elevated level of corticosterone on the organism in contrast to different paradigms of repeated behavioral or social stress. Similar to repeated restraint stress, repeated administration of exogenous corticosterone impairs the movement accuracy in skilled reaching and walking tasks [21,29]. We have previously investigated the effects of repeated corticosterone administration lasting 7 and 21 days, on field potentials evoked in layer II/III of the rat motor cortex [4]. We have reported that corticosterone treatment resulted in an increase in the field potential amplitude which occurred within less than 7 days of corticosterone administration and persisted during the following 2 weeks of the treatment. This effect was accompanied by an increase in the mean frequency of spontaneous excitatory postsynaptic currents (sEPSCs) in layer II/III pyramidal neurons, collectively suggesting an enhancement of excitatory synaptic transmission due to corticosterone treatment.
The aim of the present study was to determine whether the repeated corticosterone administration-induced functional changes in the M1 excitatory synaptic transmission are associated with structural modifications. To this end, we measured the density of dendritic spines in layer II/III pyramidal neurons in control and corticosterone-treated rats. Since stress has previously been reported to induce an increase in the level of GABAergic markers in rat mPFC [9], we also aimed at finding if corticosterone treatment lasting 7 days influences spontaneous inhibitory postsynaptic currents (sIPSCs) in M1 layer II/ III pyramidal cells. Moreover, we have also investigated the effects of a single administration of corticosterone on sEPSCs and sIPSCs and assessed the influence of prolonged corticosterone treatment on the protein level of selected subunits of AMPA, NMDA, and GABA A receptors.

Experimental procedures Animals and treatment
Experimental procedures were approved by the Animal Care and Use Committee at the Jagiellonian University and were carried out in accordance with the European Community guidelines for the use of experimental animals and the national law. Male Wistar rats, aged 5-6 weeks at the beginning of the experiment, were housed in groups and maintained on a 12-h light/dark schedule (light on: 0800-2000 hours). Standard food and tap water were available ad libitum. Animals were weighed on a daily basis. Corticosterone (Sigma-Aldrich), suspended in 1 % Tween 80, was administered subcutaneously (dose 10 mg/kg, volume 2 ml/kg) either once (termed: 1× or single administration) or twice daily, for 7 days (termed 7d or repeated administration [52]). Control animals received the vehicle (termed Tw or Tween), but otherwise they were handled identically and were investigated concurrently with corticosterone-treated rats.

Tissue preparation
In most experiments, brain slices were prepared 2 days after the last corticosterone administration to avoid acute effects of the hormone. In two sets of experiments, slices were prepared 4 or 7 days after the end of corticosterone treatment. Rats were anesthetized with isoflurane (Aerrane, Baxter) and decapitated. Their brains were quickly removed and placed in ice-cold artificial cerebrospinal fluid (ACSF) containing (in mM): 130 NaCl, 5 KCl, 2.5 CaCl 2 , 1.3 MgSO 4 , 1.25 KH 2 PO 4 , 26 NaHCO 3 , and 10 D-glucose and bubbled with the mixture of 95 % O 2 -5 % CO 2 . Coronal, frontal cortical slices (thickness 400 μm) containing a part of M1 were cut from one of the hemispheres between 3.5 and 1.7 mm rostral to bregma using a vibrating microtome (Leica VT1000). Slices were stored submerged in ACSF at 30 ± 0.5°C. Primary motor cortex (3.5-1.7 mm rostral to bregma and 2.0-3.5 mm lateral to the midline) from the other hemisphere was dissected on ice and cryopreserved at −80°C for later Western blotting analysis. In order to exclude the influence of lateralization, the brain hemispheres were used alternately for electrophysiology and Western blotting.

Whole-cell recording
Individual slices were placed in the recording chamber mounted on the stage of the Zeiss Axio Examiner. D1 microscope and superfused at 3 ml/min with warm (32 ± 0.5°C), . Pipettes had open tip resistance of approx. 6 MΩ. Pyramidal cells were sampled from sites located at least 2 mm lateral to the midline and approx. 0.3 mm below the pial surface and were identified, as described previously [45]. Signals were recorded using the SEC 05LX amplifier (NPI, Germany), filtered at 2 kHz, and digitized at 20 kHz using Digidata 1440A interface and Clampex 10 software (Molecular Devices, USA).

Electrophysiological characteristics of neurons
After obtaining the whole-cell configuration and subsequent 10-min stabilization period, the firing characteristics of the recorded cells were assessed using intracellular injections of rectangular current pulses of increasing amplitude (duration 400 ms, Fig. 1b) in the current clamp mode. For each cell, the relationship between injected current intensity and the number of spikes was plotted. The gain was determined as a slope of the straight line fitted to experimental data ( Fig. 1c; cf. [3]). The threshold current (I th ) was determined as a current extrapolated at zero firing rate (Fig. 1c).

Recording and analysis of sEPSCs and sIPSCs
To record spontaneous EPSCs, neurons were voltage-clamped at −76 mV and synaptic events were recorded for 4 min as inward currents (Fig. 2a, b). Next, cells were voltage-clamped at 0 mV and after 15 min of stabilization period, sIPSCs were recorded for 4 min as outward currents (Fig 3a, b) [44]. This approach allowed us to record from the same neuron without a need to change the recording micropipette solution. Since this method requires high amplitude current injections, what results in a greater noise level, the smaller number of sIPSCs than sEPSCs recordings was suitable for further analysis. In order to block excitatory synaptic transmission, in a separate set of experiments, sIPSCs were recorded in the ACSF supplemented with 2 mM kynurenic acid.
Spontaneous EPSCs and IPSCs were detected offline using the automatic detection protocol (Mini Analysis software, Synaptosoft Inc.) and subsequently checked manually for accuracy. Data were accepted for the analysis when the access resistance ranged between 15 and 18 MΩ, and it was stable (<25 % change) during recording. The threshold amplitude for the detection of an EPSC was set at 5 pA, and for IPSCs, the detection threshold was set at 10 pA.

Imaging and deconvolution
Following whole-cell patch clamp recordings with biocytin filling of recorded cells, slices were fixed for 24 h in 4 % formaldehyde in PBS. After several washings, tissue was incubated in phosphate-buffered saline containing 0.6 % Triton X-100 (Sigma-Aldrich) for 24 h and subsequently with 0.3 % Triton X-100 and Cy3-conjugated ExtrAvidin (1:200; Sigma-Aldrich) for 48 h. Slices were washed and mounted on glass slides, coverslipped with Vectashield containing DAPI (Vector Laboratories), and examined using the Zeiss LSM510 META confocal microscope (Microimaging GmbH, Jena, Germany). The selection scheme of dendritic segments for the spine counting was planned to minimize any possible bias. The imaging conditions were the following: 543-nm excitation wavelength generated by HeNe green laser attenuated to 60 % of its full power with Acusto-Optical Filter (AOF), 488/543 dichroic mirror to reflect excitation light to the oil immersion 63×/1.4 Plan Apochromat objective (Zeiss, Germany), 560 LP emission filter, and PMT with gain and offset adjusted to image the specimens without the clipping effect. The set of optical slices was taken for each specimen at pinhole aperture open to 1 Airy unit (106 μm pinhole diameter), 35 μs pixel time, and 604 × 604 pixels of image resolution under the 5× digital zoom to obtain Nyquist sampling criterion-X = 47 nm, Y = 47 nm, and Z = 160 nm. The experimental point spread function (PSF) for the microscope optical arrangement was calculated using images of 0.175-μm fluorescent beads (PS-Speck Microscope Point Source Kit (P7220), Life Technologies, USA). Huygens Professional software (version 4.2, SVI, Netherlands) was used for image deconvolution. PSF was calculated on the basis of at least 25 distilled and accumulated images of the beads followed by object stabilizer approach. Then, all optical sections of fluorescently tagged cells imaged in the confocal microscope (see above) were deconvolved at signal-to-noise ratio (SNR) value set to 20. Finally, the images were saved for manual spine counting from the optical stacks. Every protrusion considered to be a spine was verified on the basis of a 3D projection constructed from the stack in ImageJ (NIH). Data analyses were performed by an experimenter blinded to the treatment condition.

Western blotting
The GluA1 and GluA2 subunits were chosen for the analysis since it has been estimated that more than 80 % of native AMPA receptors are GluA1A2 heteromers [26,35]. For NMDA and GABA A receptors, we chose GluN1; GluN2A; GluN2B; and α 1 , β 2 , γ 2 subunits, respectively, since synaptic receptors containing these subunits are most abundant in the cortex [6,17,27].

Statistics
Statistical analysis of electrophysiological data was carried out using two-way ANOVA with post hoc Holm-Sidak method and post hoc Tukey test for the assessment of the longevity of corticosterone effects. Cumulative inter-event interval and amplitude distributions of postsynaptic events were compared using the Kolmogorov-Smirnov (KS) test. Western blotting and spine density data were evaluated using Student's t test. Rats were weighted on a daily basis, and body mass was analyzed using repeated measures two-way ANOVA. p < 0.05 was considered as statistically significant.  (1×, d 1 , d 2 ) or repeatedly (7d, e 1 , e 2 ). Insets-bar graphs illustrating a comparison of the mean frequency and the mean amplitude (± SEM) of sEPSCs recorded from neurons from control (white bars) and corticosterone-treated rats; once (1×-gray bars) or repeatedly (7d-black bars). ***p < 0.001. f Sample recordings of sEPSCs from a representative neuron in slice prepared from the animal receiving corticosterone for 7 days. Upper trace-recording in standard ACSF and in the presence of TTX (lower trace). g 1 , g 2 Mean frequency of sEPSCs in individual neurons before and after addition of TTX to the ACSF in slices prepared from control (g 1 ) and corticosteronetreated (g 2 ) rats
All recorded cells exhibited a regular spiking firing pattern in response to a depolarizing current pulse, typical of pyramidal neurons (Fig. 1b). There were no statistically significant differences between neurons originating from corticosteronetreated groups of animals and respective control cells either in the resting membrane potential or in the input resistance (Table 1). To assess the intrinsic excitability, the relationship between the firing rate and the intensity of the injected current was evaluated for each neuron (Fig. 1c). As illustrated in Fig. 1d, e, neither the mean gain nor the mean threshold value of action potential generation differed between experimental and control groups, indicating that corticosterone treatment did not influence the excitability of layer II/III pyramidal neurons.

Effects of corticosterone on sEPSCs and sIPSCs
A single injection of corticosterone affected neither the mean frequency nor the mean amplitude of sEPSCs (Fig. 2d 1 , d 2 ; Table 2). The occurrence of spontaneous EPSCs could be blocked by 2 mM kynurenic acid (Fig. 2b). Amplitudes and inter-event interval times of sEPSCs were plotted as cumulative distribution histograms (Fig. 2d-e). Cumulative distribution of both amplitudes and inter-event interval intervals of sEPSCs did not differ between control and corticosteronetreated groups (KS test, p > 0.05). However, in slices originating from rats treated with corticosterone for 7 days and prepared 2 days after the last hormone administration, the mean frequency, but not the mean amplitude of sEPSCs, was markedly higher than that in the cells originating from control animals receiving vehicle (Fig. 2e 1 , e 2 ; Table 2). These findings were validated by comparing data from the respective cumulative distribution histograms of sEPSCs frequencies (KS test, p < 0.001, Fig. 2e 1 ) and amplitudes (KS test, p = 0.8, Fig. 2e 2 ). The rise time and the decay time constant of the averaged sEPSCs were similar in both groups (Fig. 2c, Table 2).
To test the contribution of action potential-related glutamate release to recorded sEPSCs, the effect of Na + channel Differences between values for neurons in experimental (corticosterone) and control (Tween) groups, receiving injections once (1×) or repeatedly for 7 days (7d) are not significant (p > 0.05).
V m resting membrane potential, R m input resistance, n number of cells  blocker tetrodotoxin (TTX) was investigated in a separate sample of 6 control and 9 neurons obtained from corticosterone-treated (7d) animals (Fig. 2f) Corticosterone administration had no effect on parameters characterizing spontaneous IPSCs, which could be blocked by 20 μM bicuculline (Fig. 3b). Both the frequency and amplitude (Fig. 3d 1 -e 2 ) as well as the rise time and the decay time constant of sIPSCs (Fig. 3c, Table 3) were not significantly different from control values in any of the experimental groups. Concordantly, corticosterone treatment altered neither the inter-event interval (Fig. 3e 1 , e 2 , KS test, p = 0.5) nor the amplitude (Fig. 3e 1 , e 2 , KS test, p = 0.9) distribution of sIPSCs.
To exclude the possibility that sEPSCs contributed to spontaneous synaptic activity recorded at 0 mV, in a separate set of recordings (9 cells originating from 4 rats receiving Tween for 7 days, 10 cells from 5 rats receiving corticosterone for 7 days), 2 mM kynurenic acid was added to the ACSF (Fig. 3f). As illustrated in Fig. 3g 1 , g 2 , neither the mean frequency nor the mean amplitude of postsynaptic events recorded from neurons originating both from corticosterone-treated and control animals was affected by the blockade of glutamatergic transmission.

Corticosterone and the density of dendritic spines
For the dendritic spine counting, 11 neurons originating from rats treated with corticosterone for 7 days and 9 cells obtained from control animals were analyzed. The dendritic spines were counted on the total length of 1961.5 μm on 43 independent secondary, tertiary, and quaternary dendritic branch segments from the corticosterone group and on 1202.5 μm on 32 segments from the control group, within 10-90 μm from the soma. In total, 2496 spines on cortical neurons from corticosterone-treated animals and 1379 spines on cortical neurons from control animals were analyzed. The overall spine density did not differ significantly between the groups (1.2 ± 0.09 vs. 1.12 ± 0.07 spines/μm, respectively; p = 0.5, Fig. 4). No significant differences in spine density of basal dendrites were found between the groups (1.3 ± 0.15 vs. 1.07 ± 0.09 spines/μm, respectively; p = 0.2). The density of spines was also similar in the apical dendritic tree (1.6 ± 0.12 vs. 1.17 ± 0.11 spines/μm, respectively; p = 0.9).

Corticosterone and AMPA, NMDA, and GABA A receptor subunits
Repeated treatment with corticosterone did not alter protein levels of GluA1 and GluA2 subunits of the AMPA receptor (Fig. 5a 1 -a 2 ). Also protein levels of GluN1, GluN2A, and GluN2B subunits of the NMDA receptor (Fig. 5b 1 -b 3 ) as well as that of α 1 , β 2 , and γ 2 subunits of the GABA A receptor (Fig. 5c 1 -c 3 ) were not affected.

Longevity of the corticosterone-induced effect
In a separate group of animals, the mean frequency and amplitude of sEPSCs were compared between slices prepared 2, 4, or 7 days after the end of the corticosterone treatment lasting for 7 days. As illustrated in Fig. 6a, the mean frequency of sEPSCs measured in slices prepared 4 days after the end of the treatment was not significantly different from that measured 2 days after the treatment ended. In contrast, in slices prepared 7 days after the last corticosterone administration, the mean frequency of the sEPSCs was not different from the control, receiving the vehicle (p = 0.236). In all cases, the mean amplitude of sEPSCs did not deviate from control (Fig. 6b).

Discussion
We have previously reported that glutamatergic field potentials evoked in layer II/III of the frontal cortical area M1 by stimulation of underlying sites were increased after repeated corticosterone administration lasting 7 and 21 days. Corticosterone treatment has been found to increase the mean frequency, but not the mean amplitude, of sEPSCs in a sample of layer II/III pyramidal neurons [4]. In the present study, we have extended those findings by showing that corticosterone treatment-induced increase in the frequency of sEPSCs is absent after a single injection of the hormone when tested 2 days later and, moreover, in the same population of neurons, neither single nor repeated corticosterone injections affected sIPSCs. We also show that the sodium channel blocker tetrodotoxin does not significantly change the mean frequency of sEPSCs in layer II/III pyramidal cells of the M1 area, indicating that most of the recorded spontaneous currents correspond to action potential-independent miniature EPSCs (mEPSCs; this study and [45; 48]). The observed increase in the mean frequency of sEPSCs is consistent with a presynaptic mechanism of the effect, related to an enhanced spontaneous release of glutamate quanta from presynaptic terminals. This conclusion is supported by a lack of changes in the protein level of the tested subunits of postsynaptic ionotropic receptors as well as with a lack of changes in the density of dendritic spines. Present data also demonstrate that membrane excitability of the recorded cells remains unchanged when tested 2 days after the end of corticosterone treatment and are consistent with the observation that direct effects of corticosterone on neuronal excitability decline to baseline values within hours [16]. The influence of repeated stress or exogenous corticosterone treatment on synaptic transmission in the rat M1 has not been investigated previously. Earlier studies aimed at finding cellular and molecular mechanisms underlying the influence of stress on cortical glutamatergic transmission have mainly been focused on the medial prefrontal cortex. It was demonstrated that restraint stress (2 h/day) and unpredictable stress chosen from six stressors, when repeated for 5-7 days, induce a reduction in the amplitude of stimulation-evoked and miniature EPSCs [51]. Since these effects were accompanied by reduced protein levels of AMPA and NMDA receptor subunits but unchanged paired-pulse ratio of the evoked EPSCs, their mechanisms are mainly postsynaptic. These authors have also found that the frequency of mEPSCs was decreased, implying a contribution of a presynaptic mechanism. Another study has shown that 21 days of chronic unpredictable stress resulted in a reduction in both the frequency and the amplitude of sEPSCs, accompanied by a decrease in the expression level of several pre-and postsynaptic proteins [24]. On the other hand, Ito et al. [2010] have found no change in field potentials and mEPSCs in the anterior cingulate cortex of mice subjected to restraint lasting 2 h/day for 7 days. However, these authors observed a reduction in the frequency of IPSCs. The diverse outcomes of cited and our studies may result from the use of different experimental models but it is also likely that the responses of different cortical areas to stress are diverse. For example, while in the rat mPFC, the dendritic length and spine density decrease after stress, in the orbitofrontal cortex, they increase [22].
It has been reported that repeated corticosterone administration (40 mg/kg) for 21 days increased the immobility in the forced swim test, while repeated restraint stress had no effect on this depression-like behavior [11]. A lower dose of corticosterone (20 mg/kg per day), similar to that used in the present study, has also been found to increase immobility in the forced swim test [13]. The effects of repeated stress on the rat M1 have not yet been investigated; however, it is likely that they would be weaker than the effects of repeated corticosterone administration. The responses of the organism to repeated homotypic stress show the adaptive reduction, a phenomenon regarded as a form of habituation (reviewed in [12]). A contribution of the adaptation appears to be smaller in the unpredictable stress paradigms, however, it has been demonstrated by several authors that behavioral stress-induced corticosterone level increases are transient while repeated Fig. 4 Repeated administration of corticosterone does not change the density of dendritic spines in layer II/III pyramidal cells. a 1 , a 2 Representative images of dendritic segments of neurons originating from a control rat (a 1 ) and an animal receiving corticosterone for 7 days (a 2 ); scale bars = 10 μm. b Corticosterone (Cort) treatment lasting 7 days (7d) did not affect the density of spines on total (apical + basal), apical nor basal dendrites compared to neurons originating from rats receiving vehicle (7d Tw). Spine density is defined as the mean number of spines per micrometer of dendrite length. The differences are not statistically significant corticosterone administration-induced elevation of plasma corticosterone level lasts for about 24 h after the last injection [7,11,46]. Thus, repeated corticosterone injections are likely to exert a stronger influence on the plasma corticosterone level than repeated stress sessions. As a consequence, for example, repeated corticosterone-treated rats weigh less than the animals subjected to repeated restraint [28]. In our study after 7 days of treatment, the difference was not significant, which may be attributed to the selected dose of corticosterone (cf. [11]). Interestingly, our data show that despite a prolonged exposure to elevated levels of corticosterone, no changes were evident in the GABAergic input to layer II/III pyramidal neurons, in contrast to the glutamatergic one. On the other hand, decreased levels of GABA and GABAergic transmission have been found in the prefrontal cortex of animals subjected to chronic mild stress and chronic social defeat stress [14,47]. Again, the use of not only a different experimental model but also different responses to corticosterone of different cortical areas are likely reasons of the apparent discrepancy.
Our data demonstrate that corticosterone treatment did not influence the protein levels of GluA1, GluA2, GluN1, GluN2A, and GluN2B subunits of ionotropic glutamate receptors or that of α 1 , β 2 , and γ 2 subunits of the GABA A receptor in the rat motor cortex. Other studies showed changes in the expression levels of some receptor subunits in other cortical areas. It has been reported that repeated corticosterone administration for 14 days resulted in a decrease in the expression level of GluN2B and GluA2/3 subunit proteins in the rat medial prefrontal cortex, but no changes were evident in either adjacent lateral orbitofrontal cortex or the hippocampus [10]. Regarding the GABAergic system, corticosterone-treated rats expressed altered protein level of the α 2 , but not other subunits of the GABA A receptor in the hippocampus [28]. In contrast to the cortex, no effects of repeated stress on basal glutamatergic transmission were detected in the striatum, hippocampal CA1 area [51], and the dentate gyrus [18].
Several studies have shown that chronic treatment of rats with antidepressant drugs reduces excitatory synaptic transmission (reviewed in [31]). We have previously demonstrated that repeated administration of a tricyclic antidepressant imipramine, lasting for 2 weeks, attenuates glutamatergic transmission in the rat frontal cortex by decreasing the mean frequency and, to a lesser degree, the mean amplitude of sEPSCs [45]. Imi pramine adm inistration norm alized repeated corticosterone-induced increase in the field potential amplitude and sEPSC frequency and, moreover, restored corticosterone-impaired potential for the induction of longterm potentiation in the frontal cortex [4]. These data suggest that the influence of imipramine on glutamatergic transmission is opposite to that of repeated corticosterone administration. Our earlier work also showed that imipramine administration counteracted repeated corticosterone-induced functional modifications in the reactivity of 5-HT 1A and 5-HT 2 receptors in the rat frontal cortex [52].
Acute stress and single episodes of exposure to corticosterone transiently increase the release of glutamate from presynaptic terminals in the prefrontal and frontal cortex (reviewed in [34]). It is conceivable that repeated episodes of transient enhancement of excitatory transmission via pre-and/or postsynaptic mechanisms [50] may eventually induce a sustained modification of glutamate release via activity-dependent mechanisms.
Spontaneous excitatory postsynaptic events, previously regarded as mere synaptic noise, have been proposed to play a key role in a homeostatic regulation of synaptic efficacy by controlling dendritic protein synthesis [43]. It has been demonstrated that the level of spontaneous glutamate release adjusts the plasticity threshold at single synapses, thus representing a form of metaplasticity [23]. In line with these results, our previous study demonstrated that repeated corticosterone administration-induced enhancement of spontaneous excitatory transmission coincides with a reduced possibility for LTP induction in the rat motor cortex [4]. Of note, blockade of the NMDA component of spontaneous EPSCs has been implicated in a rapid antidepressant-like effect of ketamine and other NMDA receptor antagonists in animal models [2].
In summary, we demonstrate that repeated exogenous corticosterone administration to the adolescent rats results, by a currently unknown mechanism, in an increase in the frequency of sEPSCs, but not other parameters characterizing the glutamatergic input to layer II/III pyramidal neurons of the M1. Furthermore, we show that the GABAergic transmission remains unchanged after corticosterone treatment. These findings add to a growing body of evidence that chronically subunit proteins in the M1. Bar graphs represent summary results of the densitometric analysis of subunit proteins from the motor cortex of rats treated with corticosterone for 7 days (mean ± SEM; black bars) with respective controls, receiving vehicle (white bars). The differences are not statistically significant. In each bar, n = 6-8. The above bar graphs show computerized scans of representative Western immunoblots illustrating subunit protein bands in corticosterone-treated and control groups as well as GAPDH bands Fig. 6 Longevity of corticosterone-induced effect on the frequency of sEPSCs in layer II/III M1 pyramidal neurons. a The mean frequency of the sEPSCs recorded 2, 4, or 7 days after last drug administration from neurons obtained from animals treated with Tween or corticosterone for 7 days. *, #p < 0.05; **, ##p < 0.01. b The mean amplitude of the sEPSCs recorded 2, 4, or 7 days after last drug administration from neurons from animals treated with Tween or corticosterone for 7 days elevated corticosterone level results in a distortion of the balance between glutamate and GABA system in the brain.
In conclusion, we propose that corticosterone treatment evoked potentiation of glutamatergic transmission in the motor cortex and constitutes the potential mechanisms underlying detrimental influence of stress on skilled movement patterns and skilled movement accuracy in M1-dependent reaching and walking tasks [29].