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Creation of a Cholesteatoma Model using Three‐Dimensional Cultured Skin Equivalents

Objectives/Hypothesis: Recent years have seen success in establishing methods for cell culture that lead to the creation of a three‐dimensional tissue architecture, and this represents a great advance for the ability to carry out research in vitro. Accordingly, the present studies were carried out with the objective of using three‐dimensional cultured skin equivalents for the in vitro creation of a model of middle ear cholesteatoma, something that has heretofore been considered to be very difficult.