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Poster Sessions CP01: Phospholipids

Rat brain slices have been incubated in the presence of water-soluble synthetic peptide fragments corresponding to residues 1–9 and 1–15 of the N-terminus of immunophilin, and the effect on the phospholipid composition has been examined. During 2 h incubation in the presence of 1 nM, 0.1 lM and 10 lM concentrations of the peptides, there were significant dose-dependent decreases in the amounts of phosphatidylcholines and phosphatidylethanolamines, and increases in the amounts of phosphatidylserines (to a lesser extent of phosphatidylinositols, cardiolipins and lysophosphatidylcholines). The overall decrease in the neutral phospholipids tended to counteract any change in the phospholipid composition of the tissue. By different statistic programs as Statistica 5.5, GraphPad Prizm, the results of the experiments were analyzed and the dose-dependent model was created according to the linear regression matrix. The prediction of the influence of the synthetic peptide fragments, corresponding to residues 1–9 and 1–15 of the N-terminus of immunophilin, on the phospholipid composition, was performed based on the results of the calculations. They show that there is a linear regression within the considering variables. So, the phospholipid composition has a negative linear dependence from the synthetic peptide fragments. But the random distribution is located under the curve.