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Chromosome arm‐specific multicolor FISH

Several systems for 24‐color fluorescence in situ hybridization (FISH) have been developed and applied to karyotyping and detection of chromosomal abnormalities. We have developed a 42‐color multicolor FISH (mFISH) technique (armFISH), which permits the detection of chromosomal aberrations at the resolution of chromosome arms. The armFISH uses a commercially available mFISH reagent kit (24XCyte, MetaSystems GmbH) supplemented with a set of differentially labeled chromosome arm‐specific painting probes (arm‐kit, comprising either p‐ or q‐arms of all human chromosomes, except the p‐arm of the acrocentric‐ and Y chromosomes). The mFISH‐probe cocktail and the arm‐kit are combined and hybridized together to metaphase chromosomes. The armFISH is analyzed in two steps; first, the conventional mFISH image analysis is performed, followed by the arm‐kit analysis to reveal the chromosome arms involved. The examples demonstrate the utility of armFISH in defining chromosomal rearrangements of human cancers. © 2000 Wiley‐Liss, Inc.