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In vivo measurement of 5‐aminolaevulinic acid‐induced protoporphyrin IX photobleaching: a comparison of red and blue light of various intensities

Background: In recent years, 5‐aminolaevulinic acid (ALA) has become an increasingly popular photosensitizing drug for use in both photodynamic therapy (PDT) and photodetection (PD) of cancers. ALA metabolizes within tissue to form the photosensitizer protoporphyrin IX (PpIX). Like most photosensitizers, PpIX is fluorescent, and this fluorescence progressively decreases during PDT. This phenomenon is referred to as photobleaching.