{'fraction_non_alphanumeric': 0.04083885209713024, 'fraction_numerical': 0.011037527593818985, 'mean_word_length': 5.478571428571429, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Laser spectroscopic studies of interactions of UVI with bacterial phosphate species.

We have investigated the interactions of UVI with two bacterial phosphate-containing species: Gram-positive Bacillus sphaericus and Gram-negative Pseudomonas aeruginosa. The Gram-positive B. sphaericus was investigated by using Raman spectroscopy and time-resolved laser-induced fluorescence spectroscopy (TRLFS). We found that living cells, spores, and intact heat-killed cells complexed UVI (pH 4.5) through phosphate groups bound to their surfaces, while decomposed cells released H2PO4- and precipitated UVI as UO2(H2PO4)2. TRLFS of UVI showed that Gram-negative P. aeruginosa--genetically engineered to accumulate polyphosphate, subsequently degrade it, and secrete phosphate--precipitated UVI quantitatively at pH 4.5. The same bacterial strain, not induced to secrete phosphate, sorbed only a small amount of UVI.