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Absence of Mycoplasma species DNA in chronic fatigue syndrome.

People with chronic fatigue syndrome (CFS) suffer from multiple symptoms that include fatigue, problems with memory, concentration, sleep and musculoskeletal pain. The association of any one infectious agentwithCFS has been difficult to establish and is complicated further by the lack of a known lesion or diseased tissue to sample. Peripheral blood, by its nature of systemic circulation, serves as an ideal specimen in CFS, as it samples the entire body and could thus serve as a sentinel for diverse lesions. Plasma, the liquid portion of peripheral blood that is devoid of cells, is known to contain remnants of numerous physiological and disease processes. We used plasmaDNA to detect and characterize bacterial 16S rDNA sequences in a group of individuals with CFS and a group of nonfatigued controls (Vernon et al., 2002). Whilst a variety of bacterial sequences were detected in both fatigued and non-fatigued groups, noMycoplasma sp. 16S rDNA sequences were found. A few studies have reported the detection of several Mycoplasma species in peripheral blood cells of CFS patients by using Mycoplasma species-specific PCR (Nasralla et al., 1999; Nijs et al., 2002). In this study, we used Mycoplasma species-specific primer pairs for direct amplification of these microbes from plasma DNA.