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Activation and Suppression of the Phagocytic Activity (NBT Reduction Test) of Neutrophils in Childhood

The effect of various noxious agents on the phagocytic activity of neutrophils in childhood was studied by nitroblue tetrazolium (NBT) reduction test in vitro, and the effect was compared between newborns and older children of over one month of age. A modification of the method of Humbert and co-workers was employed for the test. Latex activated the NBT reduction activity of neutrophils in all age groups, but more markedly activated in the group over the age of one month than in the newborns. Prednisolone suppressed the NBT reduction activity of neutrophils in all age groups. Vincristine sulfate in a small dose activated the NBT reduction activity of neutrophils and suppressed in a large dose in children over one month of age; but it suppressed markedly in the newborns. Staphylococcus aureus activated, but strep-