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Comparison of HIF1 Gene Polymorphism (Rs11549465) Among Elite and Amateur Power Athletes Versus Non-athletes


 Background: One of the major energy systems for power performance in muscles is glycolysis that is regulated by the levels of Hypoxia-inducible factor-1α (Hif1) which in response to hypoxia act as a transcription factor. The goal of this survey was the comparison of HIF1 gene polymorphism (rs11549465) among elite and amateur power karate-kas versus non-athletes. Methods and Results: The C/T polymorphism allelic and genotypic's distribution of Hif1 rs11549465 gene was determined in this survey throughout 550 healthy Iranian subjects that divided in power karate-kas groups that sub-divided into elite (86 male and 86 female) and amateur karate-kas (100 male and 72 female) versus 206 individuals in non-athlete group (100 male and 106 female). After obtaining the written consent of all participants in the study, in seated mode, at a given time and in the diagnostic-laboratory, the amount of 5cc blood from brachiocephalic vein was taken. The Hif1 gene T/C polymorphism was assessed with PCR by using the forward and the overturn primers. Amplicons, the length were treated with the restriction endonuclease Hph I (Enzyme). Also RFLP were analyzed by electrophoresis separation on 1% agarose gel. Non–parametric Chi-Square and multinomial regression tests were used for assess the differences through the SPSS software and the P value less than 0.05 was considered as a significant different. Chi-square test showed genotype frequencies of Hif1 TC rs11549465 (TC genotype: 25.6% vs. TT genotype: 11.4%; CC genotype: 63%) were significantly different in these population (χ2= 99.889, P=0.000). Furthermore, the results of multiple regressions demonstrated that there isn’t general linear relationship between Hif1 gene expression and power ability of karate-kas. Conclusions: From the genetic talent aspect, there wasn't any correlation between Hif1 gene expressions with Iranian karate competitor’s levels.Key Points: Genetic and environmental factors have a great influence over components of the athletic performance such as power/ strength traits. Genetic markers may increase comprehension of athlete talent. There wasn't any correlation between Hif1 gene expressions with Iranian karate competitor’s levels.