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Regulator of G protein Signaling 14 (RGS14) Interacts with 14‐3‐3 via an H‐Ras and Gαi‐dependent Mechanism

RGS14 is a multifunctional protein scaffold that is known to interact with active Gαi/o via its RGS domain, Raf and active H‐Ras‐GTP via its R1 Ras binding domain (RBD), and inactive Gαi 1/3 via its G protein regulatory (GPR; also known as GoLoco) domain. RGS14 has been shown to suppress long term potentiation (LTP) in the CA2 region of the hippocampus, thereby regulating hippocampal‐based learning and memory. The 14‐3‐3 family of proteins has recently been shown to be necessary for hippocampal LTP and associative learning and memory. Here we show evidence for an H‐Ras and Gαi‐dependent interaction between RGS14 and 14‐3‐3. Following overexpression of FLAG‐RGS14 and 14‐3‐3γ in HEK293T cells and pull‐down of FLAG‐RGS14, we observed an interaction between RGS14 and 14‐3‐3γ only in the presence of Gαi and/or the constitutively active form of H‐Ras(G12V). This interaction appears to occur within the R1 domain of RGS14, and is corroborated by mass spectrometry analysis of proteins recovered from intact cells. RGS14 interaction with 14‐3‐3γ does not appear to be due to direct binding of Gαi or H‐Ras(G12V) to RGS14. Ongoing studies will further elucidate the nature and physiological function of this interaction between RGS14 and 14‐3‐3γ, which may give insight into the functions of both RGS14 as well as 14‐3‐3 in their roles in modulating synaptic plasticity in the hippocampus. Studies supported by R01‐NS037112‐15 (JRH) and T32‐GM008602 (KJG).