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Induction of the Hydrocephalus by the lntraventricular Injection of Aurintricarboxylic Acid in Mice Exo Utero

ABSTRACT Aurintricarboxylic acid (ATCA) was injected into the ventricle of mouse embryo on day 14 of gestation using exo utero method. Severe hydrocephalus and destruction of aqueductus cerebri were induced in the ATCA‐treated fetuses. Thinning of the cerebral cortex and widespread cell deaths in the periventricular wall of cerebrum, thalamus and hypothalamus were observed in the histological sections. The cell death was investigated immunohistochemically using an antibody against single‐stranded DNA. This antibody did not react to the dying cells, so it was suggested that the cell deaths were not apoptosis. TRPM‐2 gene expression was investigated in the hydrocephalic and normal brains on day 18 of gestation using dot‐blot hybridization analysis. However, the gene expression in the hydrocephalic brain did not increase. In the present experiment, intraventricular injection of ATCA on day 14 of gestation induced severe hydrocephalus in mice, and cell deaths in the periventricular wall were considered to be necrosis.