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PROCEEDINGS OF THE PHYSIOLOGICAL SOCIETY

The crystals shown were prepared from dog's blood by the toluene method recently described by Bradley and Sansuml. They were then placed in 10 per cent. formalin for 48 hours and mounted. In one case the hardened crystals were mixed with Farrant's solution, in the other they were spread on a slide, allowed to dry, and mounted in Canada balsam. If a solution of pure oxyhaemoglobin is treated with excess of 10 per cent. formalin no precipitate is formed, and the spectrum still shows the two bands characteristic of HbO,; therefore the method by which the formaldehyde impairs the solubility of the crystals remains an open question.