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Locations of the lip, poxB, and ilvBN genes on the physical map of Escherichia coli

The lip locus, located at ca. 14.5 min on the Escherichia coli chromosome (6), is thought to encode an enzyme (or enzymes) involved in the terminal step(s) of lipoic acid biosynthesis (1, 14). We have used a representative mutant (JRG26) from the single genetic class of lipoic acid-auxotro- phic strains described by Herbert and Guest (7) to identify the Kohara A phage which carries the complementing lip gene (Table 1). This phage also carries a DNA segment corresponding to a second genetic class of lipoic acid-auxotrophic mutants recently isolated in our laboratory (to be published elsewhere). Our restriction data from the lip locus are in general agreement with the data of Kohara et al. (9). However, we have not attempted to exhaustively verify the Kohara physical map of phage X168. Our and around The agrees (ilvBN is essentially restriction with