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Kinetics of biodegradation of 2,4‐dichlorophenoxyacetate in the presence of glucose

Biodegradation of 2,4‐dichlorophenoxyacetate (2,4‐D) and glucose were studied using a 2,4‐D acclimated culture. Biodegradation of glucose by the 2,4‐D preacclimated culture follows the typical Monod kinetics after a 20‐h lag period in the presence or absence of 2,4‐D. Biodegradation of 2,4‐D by the same culture follows Andrew's “substrate inhibition” model. When both glucose and 2,4‐D are available, mutual inhibition is observed. However, the effect on 2,4‐D is masked by the fact that larger concentrations of active biomass are produced when glucose is available. This kind of concurrent utilization and interaction results in a substantial reduction of the mean cell residence time in a continuous flow system. It also extends the area of stability of the process into higher dilution rates as well as into higher influent concentrations.