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High performance liquid chromatography-ultraviolet photometric detection for the simultaneous determination of cephalosporins in human urine and bovine milk

A simple method for the separation and determination of cefadroxil, cefaclor, cefaloxin, cefradine and cefalotin etc. was completed on a C-16 silica stationary phase packed reversed-phase column with mobile phase of water/acetonitrile/phosphoric buffer (1 mL/min) in about. 17 min. The compounds were detected directly by ultraviolet detector at 270 nm. The linear range was 0.1-40 mg/L(r >= 0.9993) and the detection limits of cefadroxil, cefaclor, cefaloxin, cefradine and cefalotin were 9.7, 6.9, 5.6, 6.4 and 4.9 mu g/L, respectively. The method was applied successfully to the detection of cephalosporins in human urine and in bovine milk with the recovery of 96.5%-105% (when added sample was 2 mg/L).