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Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein

Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses.


INTRODUCTION
Toscana virus is an enveloped negative-strand ribonucleic acid (RNA) virus that belongs to the genus phlebovirus in the Bunyaviridae family. Members of this genus also include Rift Valley Fever virus (RVFV), Sandfly Fever Sicilian virus, Severe Fever with Thrombocytopenia Syndrome virus and Uukuniemi virus. These viruses are etiological agents of a wide range of illnesses ranging from selflimiting febrile episodes to hemorrhagic fevers characterized by multi-organ failure and death (1)(2)(3)(4). The sporadic outbreaks of these viruses, coupled with their widespread distribution across all continents, make them of great im-portance to public health. The genome of the virus is encoded in three segments of varying sizes known as the large (L), medium (M) and small (S) segments that encode the viral polymerase, the envelope glycoprotein and the nucleoprotein (N), respectively (3,5). The S segment also encodes a virulence determinant known as non-structural small protein NSs in the sense direction.
The genome of phleboviruses, like other bunyaviruses, is tightly packed with N into ribonucleoprotein complexes (RNPs) (6). The RNP complex, rather than the free RNA, represents the transcription and replication template for the viral RNA-dependent RNA polymerase (RdRp). The RdRp docks on the RNP to synthesize viral messenger RNA (mRNA) and complementary antigenomic RNA (6).
Bunyaviruses replicate in the cytoplasm of host cells. For the initiation of transcription, RdRp employs an endonuclease activity to cleave and 'snatch' the caps of host cell mRNA to prime viral transcription. This mechanism has been demonstrated for La Crosse virus and, by sequence comparison, inferred for all bunyaviruses (7)(8).
Unlike other segmented viruses, members of the Bunyaviridae lack a matrix protein that connects the lipid envelope-associated glycoproteins to the RNP. Instead, in Uukuniemi virus, N interacts with the cytoplasmic tails of its cognate glycoproteins. This suggests that the bunyavirus N may, in part, fulfill the role of the missing matrix protein (9)(10). The molecular architecture and RNA-binding mechanism of several negative-strand RNA viruses have recently been characterized. The N proteins of the Rhabdoviridae and Paramyxoviridae crystallized in rings, with the RNA bound in a tunnel formed by two lobes of the same monomer (6,(11)(12)(13). The Lassa virus nucleoprotein has an exonuclease activity (14)(15) that is specific for doublestranded RNA (14,16), further indicating that nucleoproteins can have a wider role beyond acting as scaffolds for the RdRp (14)(15). Structures of orthobunyaviruses N, e.g. of Bunyamwera, Schmallenberg, Leanyer and La Crosse viruses (17)(18)(19)(20)(21), have revealed unique structural folds. In these cases, the RNA is bound in a positively charged cleft that is formed by two ␣-helical lobes. Oligomerization is mediated by both N-and C-terminal arms.
Recent studies have also provided insights into the mechanism of phlebovirus RNP assembly (22)(23)(24). The first structure of the RNA-free RVFV N showed a dimer, where an amino-terminal arm is folded back into the predicted RNA-binding pocket (24). This dimer is thought to represent an autoinhibited form of N. A second RNA-free structure of RVFV N showed a hexamer in the crystals (22). Crystal structures of RNA-bound multimers of RVFV N ranging from tetramers to hexamers showed how a polyuridine RNA sequence is nonspecifically bound between two helical lobes (23).
In this study, we have elucidated structures of the hexameric RNA-free (apo) N and of hexameric N in complex with a single-stranded nonameric RNA. Our structural studies, combined with functional experiments, suggest a molecular mechanism of how RNA binding to N induces the transition from a hexameric ring to a helical RNP assembly.

Expression and purification
The recombinant plasmids were transformed into BL21 DE3 or BL21 DE3 Rosetta cells. Bacteria were cultured in Luria Broth (LB) medium at 37 • C until an OD 600 of 0.4, followed by a temperature shift to 20 • C. The protein was produced for 18 h in the presence of 200-M isopropyl ␤-D-1-thiogalactopyranoside. Bacteria were collected by centrifugation and resuspended in lysis buffer containing 20-mM TRIS (pH 8), 10-mM imidazole, 300-mM NaCl and complete ethylenediaminetetraacetic acid-free protease inhibitors, followed by cell disruption in a microfluidizer (Microfluidics). Lysates were cleared by centrifugation at 40 000 g for 30 min at 4 • C.
The supernatant was loaded on a 30-ml gravity flow column filled with 3-ml NiNTA resin pre-equilibrated with lysis buffer. The column was washed with 20-ml lysis buffer, followed by 25-ml washing steps with equilibration buffer (20-mM TRIS, pH 8.0, 500-mM NaCl) containing increasing concentrations of imidazole (10 mM, 25 mM and 75 mM). The protein was eluted from the column using 12.5ml equilibration buffer containing 250-mM imidazole. N was further purified via size exclusion chromatography on a Sephadex 200 10/300 column (GE Healthcare) equilibrated with TRIS-buffered saline (pH 7.5).
To remove heterogeneously bound RNA from Escherichia coli, N bound to the NiNTA column was denatured by incorporating 8-M urea in the washing buffer. Bound RNA was washed off using high salt buffer (20-mM TRIS, pH 7.5, 500-mM NaCl) and N eluted by 20-mM TRIS, pH 7.5, 250-mM imidazole and 500-mM NaCl.
The fraction of bound RNA was determined by the A 260 /A 280 ratio before and after refolding. The refolded N had an A 260 /A 280 ratio of 0.6 while the native RNP complex purified directly from E. coli had a ratio of ∼1.5 indicating about 10% bound nucleic acid.
The theoretical isoelectric point of N is 9.2 (26). Cation exchange chromatography was used to further purify the refolded N and remove partially folded intermediates. To this end, the refolded protein was dialyzed against 20-mM TRIS, pH 7.5, 50-mM NaCl and bound to a MonoS column (GE Healthcare). Protein was eluted using a salt gradient from 50-mM to 1000-mM NaCl.

Circular dichroism
To verify the integrity of the refolded N, circular dichroism (CD) measurements were performed at room temperature using a protein concentration of 1 mg/ml in phosphate buffered saline, pH 7, in an Aviv 62A spectropolarimeter.

Reconstitution of the RNP complex
For reconstitution of a homogenous RNP, the refolded N was incubated at room temperature for 1 h with commercially synthesized nonameric RNA (UGUGUUUCU, IDT DNA Technologies Inc.), at a molar ratio of 1:1. A final gel filtration step was added to remove unbound RNA. The stability of the reconstituted complex was monitored by gel filtration and also on a native gel. The A 260 /A 280 ratio of the reconstituted RNP complex was 1.1, which corresponds to approximately 5% bound RNA.

Electron microscopy
N or the indicated N mutants at a final concentration of 1.5 mg/ml were mixed with an in vitro-transcribed 8-kb single-stranded RNA comprising a Green Fluorescent Protein (GFP) expression construct (27) at a final concentration of 0.15 mg/ml. The sample was 10-fold diluted with 20-mM TRIS, pH 7.5, 150-mM NaCl, and 15 l of this solution was spotted onto carbon-coated grids. The sample was stained with 2% uranyl acetate and images were acquired on a Zeiss EM910 at magnifications between 12.5 and 31.5 k.

Crystallization and data collection
Initial crystallization trials were carried out in 96-well formats using a Phoenix (Art Robbins Instruments) and Topaz system (Fluidigm Corp, South San Francisco, CA, USA). Various commercially available screens, including PACT, PEG ion, Crystal (Qiagen) and Index Screen (Hampton Research) were used. Initial hits were translated to the hanging drop technique using a drop size of 1 l + 1 l at a protein concentration of 18-20 mg/ml. N crystals were grown in 6.5% PEG 3350, 0.1-M sodium malonate, pH 4.5-5.2, while RNP crystals were grown in 11% PEG 3350, 0.1-M sodium malonate, pH 4.5-5.2. Diffraction data of single crystals were collected at BESSY-II BL14.1 and processed using XDS and MOSFLM software (28)(29).
The structure of Toscana virus N was solved by molecular replacement using a homology model of the RVFV N (PDB code 3OUO) calculated by the SWISS-MODEL server as a search model (30). Both Phaser and MOLREP programs were used (31)(32). The phase problem for the RNP was solved by molecular replacement with the refined Toscana virus N as the search model using Phaser.
Both models were built with COOT (33) alternating with refinement in PHENIX (34). For the RNP, positive density corresponding to the bound RNA was clearly visible after the first round of refinement. For both models, we employed dihedral restraints based on the high-resolution structure of N from RVFV. Strict non-crystallographic symmetry (NCS) was used at the beginning of the refinement and then gradually released. Translation, Libration and Screw (TLS) was used in the final rounds of refinement. Molprobity was used for model validation (35). Figures were prepared using Py-MOL (The PyMOL Molecular Graphics System, Version 1.3, Schrödinger, LLC) and the pymol plugin VASco (36).

Isothermal titration calorimetry (ITC)
ITC experiments were carried out at 8 • C in a VP-ITC or iTC200 (Microcal) using degassed 20-mM TRIS, pH 7.5, 150-mM NaCl. N was diluted and RNA resuspended in this buffer prior to the measurement. A 400-M solution of single-stranded 6-9-mer RNAs was titrated into a solution containing 30-M apo N. Binding isotherms were fitted and equilibrium dissociation constants were calculated using the Microcal ORIGIN software.

Deuterium exchange mass spectrometry
To obtain optimal fragmentation sequence coverage of Toscana virus N, optimal quench conditions were established as previously described (37). Deuterium exchange experiments were carried out by mixing 2 l of Toscana virus N stock solution with 6-l D 2 O buffer (8.3-mM TRIS, pH 7.2, 150-mM NaCl in D 2 O) and incubation at 0 • C for 10 s and 1000 s. At the indicated time points, the exchange reaction was quenched by adding 12 l of ice-cold optimal quench solution [0.05-M guanidinium hydrochloride, 15-mM TRIS (2-carboxyethyl)phosphine, 16.6% glycerol, 0.8% formic acid (FA)], immediately frozen on dry ice and stored at -80 • C. The non-deuterated control samples and equilibrium-deuterated control samples were also prepared by mixing protein with H 2 O buffer (8.3-mM TRIS, pH 7.2, 150-mM NaCl in D 2 O) and equilibrium-deuterated buffer (0.8% FA in 99.9% D 2 O). The frozen samples were then thawed at 5 • C and passed over an immobilized pepsin column (16-l bed volume) at a flow rate of 20 l/min, and the resulting peptides were collected on a C18 trap for desalting and separated by a Magic C18AQ column (Michrom BioResources Inc.) using a linear gradient of acetonitrile from 6.4% to 38.4% over 30 min. Mass spectrometry (MS) analysis was performed using Liquid Chromatography Quadrupole (LCQ) Classic mass spectrometer from Thermo Finnigan, with a capillary temperature of 200 • C. Data were acquired in both data-dependent MS/MS mode and MS1 profile mode, and the data analyzed by SEQUEST (Thermo Finnigan Inc.) and DXMS explorer (Sierra Analytics Inc., Modesto, CA, USA).

Structure and oligomerization of Toscana virus N
N of Toscana virus was expressed in E. coli and purified via affinity chromatography. The resulting protein was associated with E. coli nucleic acids, as apparent by the observed A 260 /A 280 ratio of 1.5. This preparation yielded protein crystals that showed only weak, anisotropic diffraction.
Using an unfolding/refolding protocol, nucleic acid-free N was obtained, as judged from the A 260/ A 280 ratio of 0.6 (Supplementary Figure S1A). Correct folding of the protein was confirmed by CD spectroscopy (Supplementary Figure  S1B). Analytical gel filtration experiments combined with static light scattering indicated a tendency of the protein to self-assemble even in the absence of RNA ( Figure 1A), unlike the N of the closely related RVFV, which exists as a dimer in solution (24).
It was previously reported that Ns of bunyaviruses bind with high affinity to the highly conserved 5 and 3 sequences of their genomic RNA segments (39). In agreement with this, N of Toscana virus bound to a nonameric singlestranded RNA sequence corresponding to the 3 genomic RNA consensus with an affinity of 560 nM in ITC experiments (Supplementary Figure S2A and Table 1). Only a weak binding signal was obtained for a 9-mer RNA with the 5 genomic RNA consensus sequence ( Supplementary Figure S2B). Interestingly, Toscana virus N bound with highest affinity (K d = 260 nM) to a control 9-mer RNA corresponding to the reverted 3 consensus sequence (termed oligo1; Figure 1B). In these titrations, a binding stoichiometry of 0.5 was derived, indicating that only every second N bound an RNA molecule. The observed RNA-binding affinities were approximately 10-fold lower compared to those reported for RVFV Ns (23). This difference is likely related to the lower salt concentration and the longer RNA oligomers used in the previous study since these authors reported similar RNA-binding affinities for Toscana and RVFV N in their experimental setup. Furthermore, RVFV N bound to oligo1 with an almost identical affinity (K D = 420 nM) compared to Toscana virus N in ITC experiments (Supplementary Figure S2H).
To further analyze the sequence-specificity of N, base pair substitutions were introduced in oligo1. The exchange of guanine 2 (G2) to adenine did not have a significant effect on the binding affinity (Supplementary Figure S2C). Furthermore, the affinity for a 9-mer polyU sequence was only slightly reduced compared to the original 9-mer sequence (Supplementary Figure S2D), indicating a certain degree of plasticity in RNA recognition.
We then reconstituted Toscana virus RNPs in vitro using an 8000-bp single-stranded RNA. By negative-stain elec- tron microscopy, we observed filamentous RNP scaffolds with a diameter of 10-12.5 nm that may have an underlying helical sub-pattern ( Figure 1C). The morphology of these RNPs was reminiscent of that of orthobunyavirus RNPs (18)(19)(20)(21)40), but differed from those reported for RVFV that showed a more extended conformation (23)(24). Crystals of N in the absence and presence of the control 9-mer RNA were obtained and diffracted to a maximal resolution of 3.3Å and 2.6Å, respectively ( Table 2). The phase problem was solved by molecular replacement. The apo structure contained 12 molecules of N in the asymmetric unit, which were organized in two hexameric rings. The RNA-containing N crystals had 24 N and 12 RNA molecules in the asymmetric unit, which were arranged in four almost identical hexameric rings ( Supplementary Figure S3A and B). The use of non-crystallographic symmetry improved electron density during refinement and allowed the unambiguous modeling of the complete 9-mer RNA in the RNP crystals. The apo structure was refined to an R work /R free of 23.9%/26.7% and the RNP structure to an R work /R free of 20.3%/24.0% ( Table 2). As in the RVFV N structure (22), each N monomer consisted of a core domain comprised of two ␣-helical lobes (the upper and the lower lobe), and an amino-terminal ␣-helical arm which was divided by a short linker, hinge II, in ␣1 and ␣2 ( Figure 1D and Supplementary Figure S4).
In the hexameric rings of the apo structure, an almost perfect six-fold symmetry between the six monomers was observed ( Figure 1E). Oligomerization was driven by the amino-terminal arm, which bound into a hydrophobic groove of the neighboring monomer at the outside of the hexamer (pockets I and II in Figure 1E and F, and Supplementary Figure S3). This interaction constituted the major oligomerization contact, as evident by the buried surface area of 1530Å 2 (the total buried surface between two monomers is 1690Å 2 ). The interaction of helix ␣1 with the neighboring monomer included hydrogen bonds and hydrophobic contacts, and a salt bridge between Lys83 and Glu29 of the opposing monomer (Supplementary Figure  S5A). A similar oligomerization mode has been reported for RVFV RNPs (22)(23). It also agrees with a recent structure of hexameric RNA-free Toscana virus N which was published during the preparation of this manuscript (23).
To probe dynamic features of the hexamer, hydrogen/deuterium exchange experiments were performed. These studies showed that in particular residues 16-24, corresponding to hinge II of the amino-terminal arm, underwent rapid hydrogen exchange in the N hexamer (Supplementary Figure S5B), pointing to a highly dynamic association of this region in solution.

Sequence-independent RNA binding in Toscana virus N
Also in the RNA-bound RNP crystals, oligomerization of N into hexamers was mediated by the amino-terminal ␣helical arm, which filled the hydrophobic groove of the core domain at the outside of hexamer (Figure 2A). In agreement with our ITC data, only every second N bound an RNA molecule, whereas the central RNA-binding cleft of the neighboring monomer was empty. In all 12 RNA-bound Ns of the asymmetric unit, the 9-mer RNA occupied a defined position in a positively charged cleft at the inside of the hexameric ring, where the first and the last residue of the RNA could clearly be distinguished in the electron density (Figure 2A and B and Supplementary Figure S6). This argues for a preferred binding mode of the 9-mer RNA to the N hexamer.
The orientation of the RNA is evident from the asymmetric distribution of the two guanine (G) bases at positions 2 and 4 which could be discriminated from the remaining pyrimidine bases in the 9-mer RNA ( Figure 2B). The RNA bases are deeply buried in the interior of the RNAbinding groove and not accessible from the outside. Some of the bases stacked against each other, for example G4 and U5, and C7, U8 and U9 ( Figure 2C and D). All bases projected toward the protein, with the exception of G4 which pointed toward the interior of the hexameric ring. Tyr32 stacks against the base of G2, while Tyr32 of the neighboring subunit stacks against the base of uridine 9 (U9) ( Figure  2C). In this way, each second N sequesters seven ribonucleotides, while two overlapping nucleotides are bound by neighboring subunits. The resulting steric restraints may explain why only every second N is occupied by RNA. In ad- dition to Tyr32, other residues contacted the RNA bases. For example, Phe35 stacks against U3, and Ser211, Ser110 and Arg191 interacted via side-chain interactions with the bases of U1, U6 and C7, respectively. Thr201 interacts with the base of G4 via a main-chain interaction ( Figure 2C). The phosphate backbone of the 9-mer RNA is recognized by several positively charged and polar residues. For example, Arg111 and Lys72 formed salt bridges to the phosphates of G4 and U6, respectively. Interestingly, Lys204 and Lys79 formed hydrogen bonds to the phosphate backbone at the 5 end of the RNA whereas they do not interact with the phosphate backbone at the 3 RNA end ( Figure 2C).
Assuming an identical conformation of the phosphate backbone, we modeled the nonameric sequence of the 5 non-coding region (oligo3 in Table 1) in the RNA-binding groove (Supplementary Figure S7). Due to the higher abundance of purine bases in oligo3, steric clashes were predicted. This may explain the reduced affinity of this sequence to N.
During the preparation of this manuscript, tetra-, pentaand hexameric structures of the RVFV N bound to 28-36mer polyU RNA sequence were reported (23). A comparison with our RNP structure indicates that the gross features of RNA recognition are conserved between Toscana virus and RVFV. Tyr32 has a direct counterpart in RVFV N (Tyr30), which similarly stacks with the guanine and uridine bases, respectively. Also the phosphate backbone in-teracts in a similar fashion with the protein in both structures; for example Lys72 and Arg111 in Toscana virus N are conserved in both viruses and form similar contacts to the phosphates. However, species-dependent differences exist. In RVFV N, Arg70 contacts the phosphate backbone whereas the corresponding alanine in Toscana virus N is not involved in RNA binding. Vice versa, Lys204 at the 5 RNA end in our RNP forms a salt bridge to the phosphate of RNA residue 1 whereas the corresponding glutamine residue in the RVFV RNP structure does not participate in RNA binding (see also below). Arg99 in the RVFV N complex makes a prominent contact with Tyr32 and additionally contacts the phosphate backbone. Somewhat surprisingly, the corresponding residue in Toscana virus N, Arg104, is not involved in RNA binding in our model. Interestingly, G4 in the Toscana virus N structure is rotated compared to the corresponding uridine in RVFV N and faces the solvent channel, which might represent a mechanism to allow base plasticity at this position. Our results suggest that the general mode of RNP assembly is conserved in phleboviruses, while minor species-specific differences may exist.

The RNA-binding tunnel
The availability of both apo and RNA-bound N structures allows for the characterization of conformational changes associated with RNA binding. In the apo-and the RNA- bound forms, the core domains of N excluding the aminoterminal arm superimpose with a root-mean-square deviation of 0.4Å indicating that RNA binding induces only minor conformational changes within the ␣-helical core. However, the amino-terminal arm of the RNA-bound subunits is considerably displaced against the N core domain when compared to the apo structure or the RNA-free Ns in the RNP ( Figure 3A and Supplementary Figure S6). In the apo N structure, Tyr32 from hinge I, Lys204 from the lower lobe and Lys79 from the upper lobe of the adjacent subunit are in close proximity to each other and cap the RNAbinding tunnel ( Figure 3B). Due to their direct RNA interaction ( Figure 3A), Tyr32 and Lys79 become separated from Lys204 by approximately 15Å (Figure 3C). Interestingly, this extensive separation is only observed at the intersubunit junction at the 5 , but not at the 3 RNA end, where Lys79 and Lys204 do not reach the phosphate backbone ( Figure 2C and Supplementary Figure S8).
Mutagenesis was performed in order to assess the importance of Tyr32, Lys79 and Lys204 for RNA binding. The Y32A mutant bound the 9-mer RNA with six-fold lower affinity (K d = 1.6 M) compared to wild-type N ( Figure  3D). In contrast, the K79A and K204A mutation did not significantly affect the RNA-binding affinity ( Figure 3D). Interestingly, the simultaneous mutation of both Tyr32 and Lys79 significantly diminished the RNA-binding affinity (K d = 6 M), more than the single Y32A mutation, suggesting that these two adjacent residues cooperate in RNA binding. All three single mutants were able to form RNPs in vitro using a synthetic single-stranded RNA as a template, although RNPs formed by the K204A mutant appeared somewhat shorter compared to wild type ( Figure  3E). The Y32A/K79A double mutant assembled into structures more reminiscent of aggregates than functional filaments, suggesting some problems in proper RNP assembly.
The importance of these residues for viral RNP activity was assessed using a system for intracellular reconstitution of viral RNPs from transfected cDNA plasmids, a so-called minireplicon assay. Since this assay has not been established for Toscana virus, we initially tested a heterologous approach using the RVFV RdRp and N of Toscana virus to drive the replication of a reporter gene (41). How-  Figure 1C. (F) Minireplicon assay of RVFV with the indicated N mutants. Cells were transfected with constructs for RVFV L, a model minigenome expressing a reporter gene, RVFV N, and the indicated N mutants. The reporter values of the minireplicon system reflect the activity of the recombinant RNPs reconstituted from expressed cDNA sequences. ever, we were unable to detect any reporter activity in this hybrid system (data not shown).
We then switched to the well-established RVFV minireplicon system and introduced mutations to equivalent residues within the RVFV N (25). While Tyr32 and Lys79 are highly conserved in phleboviruses (corresponding to Tyr30 and Lys74 in RVFV; Supplementary Figure S4), Lys204 in Toscana virus N is replaced by Gln198 in RVFV, which similarly could contact the phosphate backbone. Surprisingly, mutating the invariant Tyr30 or Lys74 to alanine did not significantly hamper reporter activity ( Figure 3F). However, simultaneous mutation of both conserved residues resulted in a stable N, which was nonetheless inactive. This suggests that Tyr30 and Lys74 work in concert with mediate viral replication. The Q198A mutant exhibited a reduced activity relative to wild type, possibly pointing to an important function in viral replication ( Figure 3F). However, western blot analyses showed that Q198A was largely undetectable, indicating a general instability.

Coupling of RNA binding to an inter-subunit rearrangement
The four hexameric rings in the asymmetric unit of the RNA-bound N hexamers were stacked in a bottom-tobottom assembly, e.g. in the opposite orientation as in the apo structure (Supplementary Figure S3). Every second N monomer was laterally displaced relative to the neighboring N leading to a staggered assembly of the hexameric ring. In this way, the six-fold symmetry in the N hexamer was broken, resulting in three-fold symmetry of the RNP (Figure 4A and B). Considerable differences were apparent in the inter-subunit assembly of N at the 5 and 3 ends of the 9-mer RNA molecules. In the following, we refer to the N interplay at the 5 RNA end, which appears to represent a functional assembly akin to helical RNP structures (see below).
Analysis of the inter-subunit assembly revealed that the position of Tyr32, and in fact the whole N-terminal arm, relative to the associating N was almost identical in the apoand RNA-bound forms ( Figure 4C). However, the simultaneous binding of Lys204 and Lys79 of the adjacent subunit to the phosphate backbone of the RNA led to a 45 • rotation between the subunits, with Tyr32 working as a pivot point ( Figure 4C and D). This domain movement created a new inter-subunit interface of 480Å 2 at the outside of the hexamer, which did not involve the amino-terminal arm (Figure 4E and F). It included several hydrogen bonds and a salt bridge between Asp173 and Arg191 from two opposing subunits, which moved 17Å toward each other. At the inter-subunit junction at the 3 RNA end, Lys204 and Lys79 did not bind RNA and occupied a similar position as in the apo form relative to the neighboring monomer (Supplementary Figure S8). Also in the RNA-and DNA-bound ringlike RNP structures of RVFV, Gln198 does not bind to nucleic acids. Consequently, no marked inter-subunit rotation is observed in these structures and no additional interface is formed (Supplementary Figure S9).
To clarify the importance of this new interface, we introduced mutations and analyzed the consequence for viral transcription and RNP production ( Figure 4G). The single point mutant R185D was expressed similarly to wild type, but exhibited greatly reduced activity in the reporter gene assay. The double mutant, D167A/R185D, was unstable and, accordingly, also did not show reporter activity. These data are consistent with an important role of this interface for RNP activity.
To further characterize this domain rearrangement, binding of shorter RNA oligomers to N was evaluated. As expected, consecutive shortening of the RNA gradually reduced the affinity for N (Supplementary Figure S2E, F and  G). Surprisingly, the binding stoichiometries of the shorter RNA oligomers (6-8 mers) did not significantly differ from the 9-mer RNAs (n = 0.4-0.5). Since each N binds seven RNA bases, the 6-mer RNA would have enough space to bind to adjacent Ns in the hexameric ring. Our data suggest that RNA binding and the associated structural rearrangements of adjacent subunits prevent binding of a second RNA molecule in the context of a hexameric ring.

Model for the helical RNPs
Our EM analysis suggested that Toscana virus N forms 10nm RNP fibers upon interaction with longer RNAs (Figure 1C). To obtain structural insights into RNP formation, we applied the observed inter-subunit shift observed at the 5 -RNA end to all consecutive subunits. This transformation resulted in a left-handed N helix with an outer diameter of 10 nm ( Figure 5). Each turn of this helix contained 5-6 N corresponding to the sequestration of 35-42 RNA nucleotides. In this model, long RNA sequences could be encapsidated in a continuous RNA-binding tunnel at the inside of the RNP involving consecutive Ns.

DISCUSSION
Phleboviruses are important human pathogens, and studying their mechanism of RNA encapsidation and replication is of vital importance. In the current study, we analyzed structures of uncomplexed and RNA-bound N of Toscana virus. These structures show that the gross features of oligomerization and RNA binding in Ns are conserved among phleboviruses (22)(23)(24). Oligomerization is mediated by an amino-terminal arm that binds in a hydrophobic pocket of the next monomer. RNA, in turn, is sequestered in a positively charged cavity at the inside of the oligomer, in a mostly sequence-independent fashion. However, it has been unclear how the hexameric planar architectures of the apo Ns of RVFV and Toscana virus are compatible with binding of longer RNA sequences. Also the RNA-and DNAbound structures of RVFV RNPs show ring-like architectures, which do not explain how longer nucleotide sequences can be bound (23).
Here, we describe a molecular mechanism how RNA binding can be coupled to an inter-subunit movement in the hexameric N inducing the transition from a ring-like to a helical RNP. Central to this inter-subunit movement is the interplay between Tyr32 and Lys204 in one subunit, Lys79 in the adjacent subunit and their interactions with RNA. In the absence of RNA, Tyr32, Lys204 and Lys79 localize closely together in the RNA-binding tunnel. At the 5 end of our 9-mer RNA, they become separated upon RNA binding. Tyr32 and Lys79 cooperate to bind the RNA from the (B) Same view on the RNP, detailing the staggered assembly induced by RNA binding. The two peripheral subunits are displaced relative to the central subunit (indicated by arrows). (C) One monomer of the apo structure (brown) was superimposed with a monomer of the RNP structure (white; this subunit did not contain 9-mer RNA) at the 5 RNA end. The relative position of the neighboring subunits is depicted (yellow: apo structure; blue: 9-mer RNAbound subunit). A magnification of the box is shown at the right. While Tyr32 of the yellow and the blue monomer occupy a similar position relative to the white/brown monomer, Lys204 undergoes an 11-Å displacement. (D) Side view of panel (A) showing that RNA binding induces a 45 • tilt between neighboring subunits. (E) View on the apo structure from the outside of the hexamer. The inter-subunit contact is almost exclusively mediated by the amino terminal arm. Arg213 stabilizes the amino-terminal arm by binding to the amide backbone of Asn28 in the same chain. (F) Same view of the Toscana virus RNP structure showing that a new inter-subunit interface is formed upon RNA binding. Arg213 forms a hydrogen bond to the peptide backbone of Leu131 while Arg217 forms a hydrogen bond to Thr137. A new salt bridge is formed between Asp173 and Arg191 of the neighboring subunit. (G) Minireplicon assay with the indicated mutants, as in Figure 3F. upper side, whereas Lys204 contacts the phosphate backbone from the lower lobe. In this way, the RNA-binding cleft is opened and a continuous RNA-binding tunnel is created. This situation is somewhat reminiscent of Lassa virus RNPs where a flexible loop regulates the open and closed conformations of the RNA tunnel (13). The conformational changes resulting from RNA binding are then translated into an inter-subunit rotation creating a new assembly interface at the outside of the oligomer. Mutations in this new interface reduce the transcriptional activity, as apparent in the minireplicon assay. We propose that the observed inter-subunit architecture at our 5 9-mer RNA represents the architecture of every inter-subunit junction in a functional RNP. The almost identical assembly of all 12 intersubunit junctions in four independent RNPs in the asymmetric unit argues for a defined state of the inter-subunit junction, rather than a random displacement of Ns in the crystal lattice. Most importantly, such architecture would allow binding of longer RNA sequences in a continuous helical RNP oligomer. To our knowledge, this is the first example describing the molecular mechanism of how RNA binding induces transition from a planar ring to a helical structure in an RNP assembly.
Why is such transition not observed in the crystal structures of RVFV RNPs or at the 3 end of the RNA molecule? Also in the RVFV RNPs, the three residues in the inter-subunit junction (Tyr30, Lys74 and Gln198) separate upon RNA binding. However, Gln198 does not contact the RNA and, consequently, no inter-subunit rotation is observed. The RNA and DNA sequences used for the crystallization of the RVFV RNP symmetrically fit into one ring at the inside of the RNP. We speculate that, in these cases, a closed ring structure might be more stable than an open helix turn and therefore be favored for crystallization. Similarly, Lys204 and Lys79 do not contact the RNA backbone at the 3 RNA end of our Toscana virus RNP and, consequently, no major rotation is observed. The 9-mer RNA may be too short at this side and/or the induction of a similar shift as at the 5 RNA end would induce an open ring and therefore be energetically unfavored. Furthermore, it can be envisaged that the proposed inter-subunit shift is energetically not favoured over the planar architecture, since we do not observe it in the apo N hexamer. Instead, it may be driven by RNA binding. This may also explain why the K204A and K79A mutants still bind with similar affinity compared to wild-type N to our 9-mer RNA--the loss of binding energy due to these mutations may be compensated by a reduced inter-subunit shift. In support of such hypothesis, the in vitro reconstituted RNPs of the K204A mutant appear shorter compared to wild type, and we see reduced activity of the corresponding RVFV N proteins K74A/Y30A in the minireplicon assays.
It has been known for some time that phlebo-and tenuiviruses are evolutionarily related (42). Both have multipartite genomes with identical 5 termini nucleotides (5 -ACACAAAG) and complementary 3 nucleotides. Their segmented genomes are both negative and ambisense and the encoded proteins show significant sequence and structural similarity. Accordingly, the phlebovirus and tenuivirus N belong to the same protein family (Pfam 05733). Furthermore, structural predictions reveal significant homology between the Ns of Toscana and Rice hoja blanca and maize stripe tenuiviruses (Supplementary Figure S10A and B) (43)(44). Interestingly, tenuivirus RNPs can assemble into helical and superhelical structures (Supplementary Figure  S10C) (45). Also the reported diameter of the tenuiviruses (8 nm) agrees well with the diameter of our RNP model helix (10 nm). We therefore envisage that our proposed mechanism for the helical assembly induced by RNA sequestration in Toscana virus RNPs is applicable to a wider range of viruses.

ACCESSION NUMBERS
Coordinates and structure factors for the Toscana virus N and N-RNA complex have been deposited in the Protein Data Bank under accession codes 4csg and 4csf. GenBank accession code: X53794 and PDB code: 3OUO.

SUPPLEMENTARY DATA
Supplementary Data are available at NAR Online.