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Principles of Resonance Energy Transfer

Fluorescence Resonance Energy Transfer (FRET) is a radiationless process in which energy is transferred from an excited donor to an acceptor molecule. For many years this principle has been exploited in flow cytometry to excite long wavelength fluorophores with short wavelength excitation; the donor/acceptor molecules are frequently known as tandem conjugates. This unit explains in careful detail the quite complex FRET process. It characterizes available parameters and instruments for FRET measurements, discusses limitations, and shows a few examples for the application of FRET, such as determining occupation of closely related receptors.