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Formation of Nano-Bio-Complex as Nanomaterials Dispersed in a Biological Solution for Understanding Nanobiological Interactions

Information on how cells interface with nanomaterials in biological environments has important implications for the practice of nanomedicine and safety consideration of nanomaterials. However, our current understanding of nanobiological interactions is still very limited. Here, we report the direct observation of nanomaterial bio-complex formation (other than protein corona) from nanomaterials dispersed in biologically relevant solutions. We observed highly selective binding of the components of cell culture medium and phosphate buffered saline to ZnO and CuO nanoparticles, independent of protein molecules. Our discoveries may provide new insights into the understanding of how cells interact with nanomaterials.

Information on how cells interface with nanomaterials in biological environments has important implications for the practice of nanomedicine and safety consideration of nanomaterials. However, our current understanding of nanobiological interactions is still very limited. Here, we report the direct observation of nanomaterial bio-complex formation (other than protein corona) from nanomaterials dispersed in biologically relevant solutions. We observed highly selective binding of the components of cell culture medium and phosphate buffered saline to ZnO and CuO nanoparticles, independent of protein molecules. Our discoveries may provide new insights into the understanding of how cells interact with nanomaterials. N anotechnology has held great promise for revolutionizing biomedicine. Nanomaterials (NMs) are widely being under investigation for bioimaging 1 , killing tumors 2 , delivering drugs/genes 3,4 . The response of biological systems to NMs depends primarily on the surface properties of NMs in a biological environment 5,6 . For example, there is increasing evidence of rapid formation of protein coronas, as NMs within biological environments acquire a coating of protein molecules [7][8][9][10][11] , and thus there is some consensus that cellular responses to materials in a biological medium reflect the adsorbed biomolecule layer, rather than the material itself. The concept of the nanoparticle (NP) protein corona is important towards understanding the dynamic surface properties of NMs in biological environments. Unfortunately, the interface issues between NMs and biological systems are too complicated and it is still not very clear how the spontaneous formation of a protein corona influences the materials' interactions with biological systems and contributes to biological outcomes of materials. On the other hand, the increasing use of NMs in industrial and consumer products has aroused global concern regarding their potential impact on the environment and human health. Although a number of studies on the effects of NMs in in vitro and in vivo systems have been published [12][13][14][15][16][17] , there are still many challenges and issues [12][13][14] encountered at the interface between NMs and biological systems when assessing the toxicity of NMs. As demonstrated here the state of NMs in biological environments is more complicated than previously thought, requiring a more holistic understanding of what happen as NMs are dispersed in the local biological environments and what cells interface in such an environment. Not only does the biological interface of NMs need to be understood and controlled, but also NMs should be treated as biological entities rather than inorganic ones 6 . The understanding of nano-bio interactions could help in promoting applications of NMs in the biomedical fields and reducing/preventing possible adverse effects to the biological systems caused by NMs.
Here, we present the first report in nanobiological field, to our knowledge, describing nanomaterial (NM) bio-complexes or ion corona formed from the binding of NMs with non-protein components in a biological environment. We observed highly selective binding of the components of cell culture medium and phosphate buffered saline (PBS) to ZnO and CuO nanoparticles (NPs), independent of protein molecules. Our findings would be helpful to understand nano-biointeractions, allowing to effectively modifying NM surfaces for specifically biomedical applications.

Results
We observed that ZnO and CuO NPs can bind various constituents from biological environments to form NM bio-complexes or ion corona. The reason that we call the formed clusters as bio-complex is that the NMs are used for biological applications, though it may not involve biological molecules. Figure 1 presents the transmission electron microscopy (TEM) elemental maps of ZnO NPs dispersed in high-glucose Dulbecco's modified Eagle's medium (DMEM) without fetal bovine serum (FBS). In addition to aggregation/agglomeration of the NPs 15 , the elemental maps clearly illustrate the binding of ZnO NPs to components of the medium to form NP bio-complexes. We detected Ca, Na, K, P, and Cl originating from the medium (components of DMEM are listed in Supplementary Information). For comparison, we examined the effects of FBS and PBS on the formation of NP bio-complexes. High-resolution TEM images ( Fig. 2a and Supplementary Fig. S1) show that in medium supplemented with 10% FBS the crystalline ZnO NPs (see Supplementary  Fig. S1) are surrounded by compounds, indicating NP bio-complex formation. Furthermore, we found that these bio-complexes also formed when the ZnO NPs were dispersed in PBS, either with (Supplementary Figs. S2-3) or without FBS (Fig. 2b). We confirmed that the observed highly ordered crystalline structures are ZnO NPs because no crystalline structures were detected in the control suspensions where no ZnO NP was dispersed in medium or PBS (Supplementary Figs. S4-7, S10). These results clearly suggest that the formation of NP bio-complexes in biological environments (medium and PBS) occurs independently of serum proteins. The formation of protein coronas and the adsorption of different proteins onto NPs affect the uptake and transport of NPs in biological systems [7][8][9][10][11] . We believe that the biological behavior of NPs could be similarly modified by the formation of NP bio-complexes.    Figure 3 displays the TEM elemental maps of CuO NPs dispersed in the DMEM supplemented with 10% FBS. By comparing the relative contrast of the maps in Fig. 1 and Supplementary Fig. S1 to that in Fig. 3, we determined that the ZnO and CuO NPs exhibited different binding affinities for the various components of the medium. As plotted in Fig. 4a, in the case of the NPs dispersed in DMEM with FBS, the ZnO NPs bound more Na than Ca and more P than Cl, whereas the CuO NPs bound more Ca than Na and more P than Cl. This selective adsorption may be similar to that seen in the formation of protein coronas, which is reported to be influenced by particle chemistry 9 . Furthermore, FBS has effort on the binding behavior of ions as we can see from Fig. 4a that the ZnO NPs which were dispersed in DMEM with FBS bound more Cl than P, indicating biological environment may also influence the binding process. In addition, as highlighted in Fig. 4b, we observed NP-like clusters that apparently do not contain CuO or ZnO NPs; we believe they arose from components of the cell culture medium. Hence, the bio-complexes and the NP-like clusters can influence size distribution of particles in the biological systems, suggesting caution should be taken as addressing size effects of NMs on biological behaviors.
To confirm the selective binding of ions by the NPs, we used X-ray photoelectron spectroscopy (XPS) to investigate the ensemble of ZnO NPs and CuO NPs after dispersed in the DMEM or DMEM with FBS. Table 1 summarizes the atomic concentrations of the main elements bound by the NPs. In the case of the NPs ensemble dispersed in the DMEM with FBS, the orders of estimated atomic concentration of elements bound by ZnO NPs and by CuO NPs are Na1s (2.8%) . P2s (0.8%) . Ca2p (0.7%) . Cl2p (0.6%) and Na1s (2.6%) . Ca2p (1.8%) . P2s (1.0%) . Cl2p (0.5%), respectively. From Table 1, we also found that FBS affected the ion binding as compared the ZnO NPs dispersed in DMEM to that dispersed in DMEM with FBS. These XPS results are in good agreement with the TEM observation on the individual NPs.
Although we did not detect obvious signal of N, a main component of protein, by TEM, we detected it by XPS due to much lower energy of the used X-ray than the electron beam used for TEM observation. The N1s atomic concentration of the ZnO NPs dispersed in DMEM with FBS is much higher than that of the ZnO NPs dispersed in   DMEM. Furthermore, the binding energy of one of the fit peaks of N1s spectra shifted to 399.76 eV (Fig. 5b) or 399.70 eV (Fig. 5c) from 399.41 eV of the pristine FBS ( Supplementary Fig. S15b and Table  S1). The shift suggests protein molecules in the FBS may bind to the NPs, forming protein corona.
As shown in Figs. 5d-f and Supplementary Figs. S15c-d, we detected different shifts, toward a lower binding energy in contrast to a higher binding energy of the N1s (Figs. 5b-c), of the Na1s and Cl2p binding energies as they contacted with ZnO NPs in DMEM or DMEM with FBS from the pristine DMEM and FBS. The different shift may indicate various interactions between the ions and ZnO NPs in the different solution environments. The various interactions reflect the change of zeta potential of the NPs dispersed in the different solvents as shown in Fig. 6. For instance, the zeta potential of ZnO NPs changed from a negative value in de-ionized water (DI water) to a positive value as dispersed in DMEM or DMEM with FBS. The change of zeta potential of ZnO and CuO NPs is believed to be due to the binding of ions from the solvents, which is consistent with the selective binding of ions by the ZnO and CuO NPs.

Discussion
We have shown that ZnO and CuO NPs formed bio-complex or ion corona by selectively absorbing components or ions from the DMEM, DMEM supplemented with FBS, and PBS. The formation of bio-complexes is not associated with serum proteins. The discovery of bio-complex formation offers new insights for interpreting the observation that the uptake of negatively charged NPs by cells is greater than that of non-functionalized NPs 18,19 . This phenomenon is contradictory to the well-established concept that positively charged NPs have high affinity to the negatively charged cell membrane, and the unfavorable interaction was partially attributed to the formation of NP protein corona 19 . For instance, it has recently been    shown that negatively charged Au NPs are more toxic than positively charged and neutral Au NP counterparts, due to the interaction of the outer Au NP components with cellular components 20 . The interaction of the negatively charged Au NPs with cellular components could in turn influence the mitochondrial membrane potential by releasing positively charged calcium ions from the matrix into the cytosol 20 . Thus, previous work related to the effects of surface charge and surface modification on cell-specific responses and cytotoxicity 21-23 may need to be re-evaluated. That is, the surface may not the one as expected from the intended surface modification. Our finding that NP bio-complexes form in biological environments could be used to help design the NP surfaces for effective drug, DNA and siRAN delivery systems and more accurate nanotoxicity studies. We did not detect signals from key components of serum proteins, such as N, due to the high energy of the electron beam used in the TEM technique. However, we did observe the disappearance of certain matters during the TEM observation because biomolecules can be easily damaged by high energy beam, which may have been the serum proteins. Previously, Maiorano et al. made the assumption that an observed cloud surrounding Au NPs was caused by proteins, based on TEM observations without corresponding elemental maps 24 . Despite this assumption, protein coronas can mediate cellular responses 6 and the toxic effects of NMs 24,25 . We believe that identification of the constituents of NP bio-complexes would significantly improve our understanding of what is selectively acquired by NMs in biological environments; how those acquired constituents influence the reactivity, transport, transformation, bioavailability, and toxicity of NMs; and how the NMs interact with living cells 26,27 .
Although there is implication that the binding components from cellular environment had great effects on the toxicity of Au NPs 20 , it is currently impossible to describe with certainty all the interactions play at the nano-bio interface in biological environments. The concept of the NP protein corona is important in understanding the dynamic surface properties of NMs in biological fluids. However, even at this moment, it is less clear how the spontaneous formation of a protein corona influences the materials' interactions with biological surfaces and receptors and contributes to biological outcomes of materials. Although it is not surprising that NPs would aggregate in salt rich solutions as this is well known from colloidal theory, the present observation of NMs bio-complexes is certainly new, and such kind of ion corona may be assumed as protein corona if there is no elemental identification 24 . We observed the formation of bio-complexes after naturally drying of the ZnO and CuO NPs immersed in the biological solutions. It remains unclear how dynamically biological environments influence the attaching and detaching of the components from the solution, which is under investigation. Despite challenges to probe events happened at the nano-biointerface, the basic principles of nano-biointerface are being investigated by establishing new imaging techniques, such as TEM and scanning probe microscopy, and biological approaches 5 . The well known colloid chemistry and physics might be considered to be helpful to understand the formation of bio-complexes, however, the behaviors in a biological environment is conceptually different from the wellknown colloidal phenomena 6 . By contrast, the methods for protein corona investigation may be extended to explore dynamics of biocomplexes 26,27 .
In summary, we have discovered the formation of NM bio-complexes originating from the adsorption of various components in a biological environment onto NMs, independent of proteins. The interaction between NMs and the local environment adds complexity to the challenge of determining the biological outcome of using NMs. Thus, identification of the constituents of these bio-complexes would provide new insights into our understanding of how NMs are modified in biological environments and what cells really see. These results are expected to improve our understanding of the interactions between NMs and live cells. The results of our study are a reminder that our understanding of the nano-bio interactions is still in its infancy.

Methods
Nanoparticles. High-purity ZnO and CuO NPs were obtained from Sigma-Aldrich (Sigma-Aldrich Co., Japan). The primary dimensions and sizes of the oxides were provided in the data sheet, as determined by X-ray diffraction (XRD) and Brunauer-Emmett-Teller (BET) analysis. The average sizes of the ZnO and CuO NPs were 60 nm and 50 nm, respectively, but were not uniform, as previously observed by electron microscopy 16 . In contrast to Au and silica NPs, it is almost impossible to synthesize homogeneous ZnO or CuO NPs with identical shape and size. We characterized the primary size, shape, and composition of the NPs by scanning electron microscopy (SEM) equipped with energy dispersive spectroscopy (EDS) (JSM-7001F, JEOL Inc., Japan) and by X-ray photoelectron spectroscopy (XPS) (PHI Quantera SXM, ULVAC-PHI, Japan) 16 . Note that here we used the averaged primary size as many reports by others 17 . In the regard of varying size of the ZnO and CuO NPs, our observed formation of bio-complex is independent on the size of materials.
Nanoparticle suspension preparation. ZnO and CuO were dispersed in medium with FBS, medium without FBS, PBS, or PBS with FBS. The concentration of the NP suspensions was 25 mg/mL. TEM characterization. The dark-field TEM images, high-resolution TEM (HRTEM) images, elemental mapping, and EDS were analyzed using a JEM-2100F highresolution transmission electron microscope (JEOL Inc., Japan) with acceleration voltage of 200 kV. The samples for TEM observation were prepared by immersing TEM mesh into the NP suspension (25 mg/mL) for 24 h at 37uC in the dark with 5% CO 2 , the typical cell culture conditions. After naturally drying in air, the TEM mesh was observed.
XPS characterization. PHI Quantera SXM (ULVAC-PHI) with monochromatic Al Ka X-ray (1.530.1 mm, 100 W) (pass energy of 55 eV and step of 0.2 eV for narrow spectrum and pass energy of 280 eV and step of 0.5 eV for survey spectrum) was used. Binding energy corrections for the spectra were carried out by using C1s peaks at 285.0 eV. The sample for XPS investigation was prepared by immersing Au substrate into the suspension of NPs in DMEM or DMEM with FBS.
Zeta potential. The surface zeta potentials of the ZnO NPs and CuO NPs were measured by using a laser electrophoresis zeta-potential analyzer (LEZA-600, Otsuka Electronics, Japan). The NPs were dispersed in the solvents with a concentration of 25 mg/mL by sonication for ,60 min to reduce aggregation/agglomeration. The cell unit of the equipment was washed by using the same solvent as that for dispersing NPs prior to measurement. We measured three times for each sample.