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Urban tropical forest islets as hotspots of ants in general and invasive ants in particular

Urbanization is a crucial driver of environmental and biodiversity change. It is suggested that urbanization favours generalist and invasive species and might harm specialists of natural and semi-natural habitats. In this study, we examined how an urbanization gradient and environmental gradients in the habitat area, habitat diversity, elevation, and proportion of built-up area influenced the abundance and richness of ants within tropical forest islet habitat in south India. We used abundance (proportional trap incidence) of overall ants, native ants, invasive ants, and Anoplolepis gracilipes—a globally notorious invasive ant of possible south Asian origin—and rarefied richness as the response variables. We found that native ant abundance was greater and A. gracilipes abundance was lesser in less-urbanized landscape compared to moderately-urbanized and highly-urbanized landscape. The richness of ants and abundance of overall and invasive ants were unaffected by the urbanization. We also found that none of the measured environmental gradients but habitat diversity influenced abundance of overall ants, native ants, overall invasive ants, and richness of ants; however, A. gracilipes abundance was negatively correlated with habitat diversity. Ant species composition of less-urbanized landscape was distinct from that of higher urbanization levels. The richness and abundance of native ants and abundance of non-A. gracilipes invasive ants decreased with the abundance of A. gracilipes. Because the forest islets of all three urbanization levels supported similar richness of native ants, the urbanization seems not to have an adverse effect for the native ants of native forest islets. The increasing population of A. gracilipes in urban green islets, however, is a concern. Future studies might investigate its effect on other invertebrates of epigeal and soil strata.

gardens and parks that use no hazardous chemicals to manage insect pests or rodents are particularly rich and abundant on native ant species [21][22][23]26 . Sacred groves-the relics of biodiversity-rich natural forests [27][28][29] have a higher ant diversity 25,30,31 . Although a good number of studies are in agreement that urbanization can change the characteristics of ant communities [16][17][18][19][20][21][22] and favour invasive ant species in urban green spaces 15,30,32,33 , Nooten et al. 26 found that the sacred groves of urban Hong Kong encompass a higher richness of native and specialist ants, which are resilient to invasive species. Invasion of tramp or invasive species results in colonization and spill-over to neighbouring less-disturbed areas, where they might displace or affect the activities of native ants 15,30,32,33 . Our pilot studies showed that the plausible native, but globally notorious invasive ant species, the Yellow-Crazy Ant (Anoplolepis gracilipes), increased several folds in urban sacred groves and spilled over to the rural sacred groves of northern Malabar region over a period of 6 years 30,34 . Conversely, in the southern United States, the invasive fire ant-Solenopsis invicta-translocated the native fire ant-S. geminata-from the natural forests to urban green spaces 33 . Therefore, the effect of urbanization on ant activities could be site, species, and community specific.
In the present investigation, we examined how an urbanization gradient and environmental gradients in the habitat area, habitat diversity, elevation, and proportion of built-up area influenced the abundance and richness of epigeic ant communities of sacred groves-natural tropical evergreen forests protected by the religious sentiments-in south India. We specifically asked the following questions: (1) what is the effect of urbanization and other habitat gradients on abundance, richness, and community structure of overall epigeic ants? (2) whether the urbanization favours the invasion of invasive ants in general and Yellow-Crazy Ant in particular? and (3) whether the native ant communities of the sacred groves were resilient to the invasion by the Yellow-Crazy Ants? In the entire study, we gave a special status for the Yellow-Crazy Ant because it is one of the 100 globally notorious invasive species 35 and a rapidly range expanding plausible native species of south India 30,31,34 .

Study sites.
To test for the association between urbanization and ant activities of the sacred groves, we selected thirty sacred groves across three districts-Trivandrum, Kasaragod, and Kodagu-in the southern Western Ghats biodiversity hotspot that experience the urbanization of different degrees (Fig. 1). The minimum and maximum aerial distance between two locations were 70 km (Kodagu and Kasaragod) and 450 km (Kasaragod and Trivandrum). Kodagu is located on the forested landscape of the Western Ghats at an elevation of > 700 m asl. Kasaragod and Trivandrum are located on the fringes of the Western Ghats at an average elevation between 19 m asl and 135 m asl (Table S1). In each of these locations, ten independent sacred groves (hereafter, sites) were selected. The mean distance between two independent sites was about 8 km for Kodagu, 14 km for Kasaragod and 13 km for Trivandrum. Sacred groves. Historically, the sacred groves of Kerala and Karnataka in south India are the remnants of the natural forests that have been instituted by the religious sentiments during the widespread deforestation practices happened during the colonial period centuries ago. Therefore, their age and the way they are worshipped and conserved are the same. However, due to post-independent agricultural intensification, human migrations, and urbanizations, the sacred groves have been isolated as green islets in the middle of anthropogenically altered landscapes. Regardless of the locations, the sacred groves contain relics of evergreen forests of the size < 10 ha. While the floristic aspects of the biodiversity of the sacred groves of these States are well-known 36,37 , with our investigations, the faunistic aspects of the biodiversity of the sacred groves are just coming to the light 30,31,34,[38][39][40] . While the sacred groves of Kodagu are located on a land mosaic of human settlements, business districts, homeyards, coffee plantations, and protected forests, the sacred groves of Kasaragod and Trivandrum are located on a land matrix of human settlements, business districts, and Coconut-based orchards or homeyards.
Urbanization. On the degree of urbanization, Trivandrum (T-HU) represents the highly-urbanized district with a population of 1509 people per km 2 , Kasaragod (K-MU) represents the moderately-urbanized district with a population of 656 people per km 2 , and Kodagu (C-LU) represents the less-urbanized district with a population of 135 people per km 2 . Urbanization, however, was quantitively assessed by calculating the proportion of the total area under built-up conditions in one-kilometre radius from the boundary of the sacred groves into the surrounding landscape. We used remote sensing techniques to calculate the proportion of the built-up area. We used data from the Sentinel 2 procured from USGS Earth Explorer (https:// earth explo rer. usgs. gov) for the present study. Images of SENTINEL 2A were used for Kodagu and Kasaragod and images of SENTINEL 2B were used for Trivandrum. Supervised classification using Maximum Likelihood Classifier was conducted to estimate the built-up area, which was measured on a hectare scale. Normalized Difference Vegetation Index (NDVI) was calculated for all the sacred groves, which indicates the vegetation health/ habitat diversity of the sacred groves. NDVI = (NIR − RED)/(NIR + RED), and the values ranged from − 1.0 to 1.0. Ant sampling. Ants were sampled during the dry (February-March) and wet seasons (August-September) of 2017. Together, we installed a total of 600 pitfall traps (plastic jars of 10 cm diameter and 15 cm depth) in the thirty sacred groves, but retrieved only 588 traps as some were damaged or lost to the public/wildlife. In each sacred grove, ten pitfall traps were installed on a linear transects in the core area of the groves. We installed the traps in a zigzag fashion along a linear transect. The inter-trap distance was about fifteen meters. We negotiated fallen trees, rainwater channels, termite mounds, ant nests, or any other disturbances that can affect the trap catches, while selecting spots for installing the traps. The traps were installed flush with the ground in such a way that the trap mouth opens at the ground level. We used 50 ml of 70% isopropanol as a preservative in the pitfall traps. The traps were operated for 5 days continuously, retrieved on the sixth day. We poured the contents www.nature.com/scientificreports/ in a glass Petri dish, and sorted the ants out from the rest of the catches and debris using a 40× stereo zoom microscope. The ants were either identified to species or to genus and morphospecies for complex groups such as Pheidole spp. following Bingham 41 and other species descriptions available in literature 42 . Ant species were subsequently grouped into native ants, invasive ants other than the Yellow-Crazy Ant (hereafter, non-YCA invasive ants), and the Yellow-Crazy Ant.

Environmental variables.
To determine the important local environmental variables and the possible ecological correlates of ant communities in the sacred groves, we collected data on forest area (from the records of the authorities), canopy closure level (on ranked visual scales 43 ), number of big trees (> 30 cm DBH) and all trees of the entire sacred groves, and leaf-litter bed size for each of the thirty sacred groves during the dry season of sampling. To measure the canopy closure level and leaf litter bed depth, four spots in the vicinity of the linear transect were used. The canopy closure level was visually estimated on a percentage scale and the litter bed depth was measured using a steel centimeter ruler. The mean values of the four quadrants were used in the statistical models. In order to determine the best ecological covariates of ant activities in the models, we examined the relationships among these variables using the generalized/linear models with appropriate distribution type fitted as the error in the models. The litter depth (F 1,28 = 4.45, p = 0.043; R 2 = 0.11), canopy closure level (F 1,28 = 11.35, p = 0.002; R 2 = 0.26), number of all trees (F 1,28 = 115, p < 0.00005; R 2 = 0.79), and number of big trees in the sacred groves (F 1,28 = 70.5, p < 0.00005; R 2 = 0.71) increased with the area of the forest. Therefore, in the predictive models we used the forest area as a local driver of the ant activities in the sacred groves. environmental variables associated to sacred groves varied for the locations of three urbanization levels. Wherever the count was the nature of response variable, Poisson distribution was fitted and wherever the response variable was continuous in nature, Gaussian distribution was fitted as the error. The significance of the models was tested using one-way ANOVA available in the R-package, car 45 . The sampling completion was examined using individual-based sample coverage estimator of species richness for each location using the R-package iNEXT 46 . We were interested in understanding how urbanization level affected ant abundances and richness. Site was considered as an independent replicate; therefore, ant data from two seasons were combined to form a single site × species matrix. Because the number of individuals collected in pitfall traps could be affected by the proximity of the traps to nests, we used trap incidence to construct the site × species matrix 47 . To account for the loss of pitfall traps to wildlife and people in some sites, the proportional trap incidence was used in the matrix. Therefore, proportional trap incidence was used as the representative of abundance in the whole analyses. The proportional trap incidence of a species was calculated by dividing the number of traps that captured individuals by the total number of traps retrieved at each site. The proportional trap incidence of each species was bounded between 0 and 1.
To determine how ant abundance per site was affected by urbanization level, proportional trap incidence of all species was averaged and used as the metric of ant abundance at the site level. We repeated this to find out the abundance of overall ants, native ants, overall invasive ants, Yellow-Crazy Ant, and invasive ants but Yellow-Crazy Ant. To determine how the diversity of ant species was affected by urbanization level, we estimated species richness rarefied by the minimum incidence of ants at each site. The rarefied richness was highly correlated with the observed richness (R 2 adj = 0.61). We were also interested in understanding how the measured landscape variables were driving the ant variables along with the urbanization level; therefore, we fitted proportion of built-up area around the sacred groves, habitat diversity of sacred groves (NDVI), area of the sacred groves, and elevation of the sites as the covariates in the models. We used a linear model (lm) with the urbanization level (highlyurbanized/ moderately-urbanized/less-urbanized), NDVI of sacred grove, elevation, area of sacred grove, and proportion of built-up area around sacred groves as the predictor variables. We arcsine square-root transformed the proportional trap incidence of each ant variable but the rarefaction estimates of species richness, square-root transformed the NDVI, and log-transformed the elevation and area of sacred groves to meet the assumptions of linear model fitting. We did not find significant interactions among the predictors; therefore the final fitted model was "model < − lm(asin(sqrt(y)) ~ urbanization level + sqrt(proportion of built-up area) + sqrt(NDVI) + log(area of sacred grove) + log(elevation))". The significance of the overall model was tested using Anova available in the R-package, car 45 . The residuals of the fitted final models were tested using the R-package DHARMa 48 .
We were interested in understanding whether the abundance of Yellow-Crazy Ant in sites was driving the abundance of native ants and other invasive ant species. To represent the abundance of both the ants, proportional trap incidences were used in the linear models. The proportional trap incidence of native or other ants were used as the response variable and proportional trap incidence of Yellow-Crazy Ant was used as the predictor. Both the variables were arcsine-square-root transformed to meet the assumptions of linear modelling.
To test for the differences in the ant community composition among sites of different urbanization gradients, we used a paired permutational multivariate analysis of variance (PERMANOVA) using the 'adonis' function and 999 permutations. This analysis was performed in the R-package 'vegan' 49 . In the PERMANOVA, the Jaccard distance matrix of species composition of overall ants was used as the response variable, with the urbanization level (highly-urbanized, moderately-urbanized, and less urbanized) as a fixed factor. In the model, the strata argument was set to 'site pair' for the randomizations to occur within each pair. We performed this analysis for overall ants. We used non-metric multidimensional scaling (NMDS) available in the R-package 'vegan' to visualize the variations in ant community among urbanization levels. All analyses were performed in R v.3.2.3 50 .

Results
Species diversity. Sixteen thousand forty-one ants belonging to 96 species and eight subfamilies ( Table 1 and Table S2) were collected. The rarefaction and sample coverage estimators suggest that the present sampling was near to completion ( Fig. 2; Fig. S1). Out of overall ants collected, 11,823 individuals (74%) belonged to 86 species and 4,208 individuals (26%) belonged to 10 species were native and invasive ants, respectively. Of the ten invasive species collected in the study, the Yellow-Crazy Ant's share alone was about 50% (Table 1). Lepisiota Table 1. Summary of ants sampled in three locations representing the levels of urbanization (LU = lessurbanized; MU = moderately-urbanized; HU = highly-urbanized). The percent share of native and invasive ants is given. The percent share of A. gracilipes is on the total abundance of ants.    Fig. 3 and Fig. S4). The sacred groves of all the three urbanization levels, however, had similar abundance of overall ants (F 2,23 = 2.3, P = 0.12) and richness of native ant species (F 2,23 = 1.7, P = 0.19). Similarly, the abundance of overall invasive ants and non-A. gracilipes invasive ants was similar in the sites of the three urbanization levels (Figs. 3 and S4). None of the measured covariables but the habitat diversity of the sacred groves (NDVI) affected the ant variables ( Table 2, Fig. S2). The abundance of A. gracilipes decreased with the NDVI of the sacred groves (F 2,23 = 4.7, P = 0.04).
Ant communities in the sacred groves along the urbanization gradient. The epigeic ant communities of the sacred groves of the three urbanization levels was different (PERMANOVA: Pseudo-F = 6.4, R 2 = 0.32, P = 0.001; Fig. 4); the less-urbanized sites stood markedly away from the highly-urbanized and moderately-urbanized sites. Twenty-one species of ants belonged to Myrmicinae (11 species), Ponerinae (4 species), Formicinae (5 species), and Dolichoderinae (1 species) dominated the entire ant community of the sacred groves of the three urbanization levels. Among the ten most dominant ant species of the respective ant communities (Fig. S5), five species belonged to Myrmicinae (Pheidole sp15, Pheidole sp18, Tetramorium mixtum), Formicinae (A. gracilipes) and Ponerinae (Odontomachus haematodus) were common to moderately-urbanized and highly-urbanized sites, three species belonged to Myrmicinae (Pheidole sp15) and Formicinae (Oecophyla smaragdina and Nylanderia indica) were common to less-urbanized-and moderately-urbanized sites, and one species belonged to Myrmicinae (Pheidole sp15) was common to less urbanized and highly urbanized sites. The latter www.nature.com/scientificreports/ species was also common in the sacred groves of all the three urbanization levels and was the most collected one in the highly-urbanized and moderately-urbanized sacred groves. However, in the highly-urbanized sacred groves, A. gracilipes replaced Pheidole sp15 by a remarkably higher margin (Fig. S5).

Effect of Yellow-Crazy Ant on other invasive ants and native ants.
In all the three locations, one or several species of invasive ants were present in the sacred groves (Figs. S5 and S6). As far as A. gracilipes (Yellow-Crazy Ant) was concerned, it was collected only in three out of the ten sacred groves of the less-urbanized landscape but was collected in all the ten sacred groves of the moderately-and highly-urbanized landscapes. The ratio of A. gracilipes to the native ants on the proportional trap incidence of ants increased from the less-urbanized sacred groves (0.02) to moderately-urbanized (0.44) and highly-urbanized sacred groves (0.61). The share of A. gracilipes in the abundance of overall invasive ant species was 2.7%, 55%, and 86% in less-, moderately-, and highly-urbanized locations, respectively. The proportional trap incidence of A. gracilipes and Paratrechina longocornis (Longhorn-Crazy Ant) decreased from the highly-urbanized sacred groves to the less-urbanized sacred groves. For Monomorium floricola and Tetramorium lanuginosum-the other two invasive species-the proportional trap incidence increased from the highly-urbanized sacred groves to the less-urbanized sacred  www.nature.com/scientificreports/ groves. Tapinoma melanocephalum was collected similarly in the sacred groves of all three urbanization levels.
Odontomachus haematodus was not collected in the sacred groves of less-urbanized sacred groves, but collected most in the moderately-urbanized sacred groves (Fig. S6).
The proportional trap incidence of native ants decreased with the proportional trap incidence of A. gracilipes (F 1,28 = 6.07, P = 0.02; R 2 adj = 0.15). The richness of native ants also decreased with the abundance of A. gracilipes (F 1,28 = 6.74, P = 0.01; R 2 adj = 0.17). The abundance of combined non-A. gracilipes invasive ants decreased with the abundance of A. gracilipes (F 1,28 = 4.71, P = 0.04; R 2 adj = 0.17). When we analysed the relationship between A. gracilipes and five most dominant invasive ant species, we found that P. longicornis

Discussion
We tested whether the abundance and the richness of native and invasive epigeic ants of the tropical forest islets of the sacred groves were affected by the urbanization and whether the abundance of the native ants and other invasive ants was affected by the abundance of Yellow-Crazy Ant-an invasive species responded positively to the urbanization. The most striking findings of this study are that (a) the urbanization unaffected the richness, but negatively affected the abundance of native ants; (b) the urbanization, despite unaffected the abundance of overall invasive ants, favoured the abundance of A. gracilipes-the Yellow-Crazy Ant-and Paratrehina longicornis-the Longhorn-Crazy Ant-the two invasive ant species of economic and ecological importance; (c) the abundance and the richness of native ants decreased with the abundance of A. gracilipes; (d) the abundance of A. gracilipes had mixed effects for other dominant invasive ant species; and (e) the sacred groves of less-urbanized sites are different from the sacred groves of the moderately-and highly-urbanized sites on the composition of ants.
In our predictive models, we used the proportion of the built-up area around the sacred groves as a quantifiable measure of urbanization and disturbance, the NDVI as the measure of the habitat diversity of the sacred groves, the altitude and the area of the sacred groves as the potential covariates of ant abundance and diversity. We fitted altitude and area of the habitat as the drivers because they have ranged considerably in the present study and are often depicted as the drivers of ant abundance and diversity. The models, however, found that none of the co-variables were crucial predictors of the abundance and richness of overall ants and different functional guilds of the ants. However, the vegetation health of the sacred groves (NDVI) was a crucial driver of A. gracilipes, which was lesser for the sacred groves of higher NDVI values.
As expected, the abundance of the Yellow-Crazy Ant increased in the sacred groves with the proportion of the built-up area around the sacred groves. The trap incidence of the Yellow-Crazy Ant was highest for the sacred groves of the highly-urbanized sites. Its trap incidence in the sacred groves of the moderately-urbanized site was increasing dramatically over the years 30 . It is suggested that the invasive ants, including the Yellow-Crazy Ant increase their population quickly in more disturbed environments than the less disturbed areas in the naturalized and invaded regions 17,30,31,51 . The sacred groves of the moderately-and less-urbanized sites were similar on the vegetation health. Our findings that the Yellow-Crazy Ant was hardly collected in the sacred groves of the less-urbanized sites agree to the patterns observed for the invasive ants along the disturbance gradients by previous studies elsewhere 17,30,31,51 .
Studies often suggest that urbanization may not have an adverse effect for social insects, such as ants 22,52-54 and bees 55 . On the diversity of the native ants of the sacred groves, urbanization seems not to have an adverse effect in the present study. The effect the urbanization had on the abundance of the native ants was marginal. These www.nature.com/scientificreports/ patterns suggest that the sacred groves, regardless of their size, can conserve the native ants even in the highly urbanized areas. Our findings agree with Melliger et al. 51 , who found that the size of the urban green spaces is a poor predictor of surface-active ants. The other possible, rather an indirect, channel the urbanization can adversely affect the native ants is through favouring the population of certain invasive and tramp ant species and giving a competitive displacement of native ants [56][57][58][59][60] . In our study, urbanization has driven the population of A. gracilipes; it was collected abundantly in the highly and moderately urbanized sacred groves than the less-urbanized landscape. The urban environment is always dynamic, therefore native ants with sophisticated and modular communication skills are better suited to lead an urban life 52,61 . However, their ability to thrive in the urban green islets might be partly driven by the nature of the dominant urban-adapted native and invasive species. The findings of the present study also suggest that apart from the Yellow-Crazy Ant, the Longhorn Crazy Ant and Odontomachus haematodus also might have adapted to live in the urbanized natural areas. They were collected abundantly in the sacred groves of the highly urbanized landscape, but were collected by a negligible number in the less-urbanized sacred groves and moderately in the moderately-urbanized sacred groves. Although A. gracilipes was confined only in the three sacred groves and collected in a very few numbers of the traps in the less-urbanized landscape of Kodagu, the invasion may be observed seriously. Our 6-year-long monitoring of the Yellow-Crazy Ant in the groves and agroecosystems of Kasaragod-the moderately urbanized site of the present study-found an exponential growth and spill-over of its population to newer sites including agroecosystems and hindrance of pollination services in vegetable crops 30,31,62 .
Among the invasive ant species collected in the present study, both the species of Crazy Ants-A. gracilipes and P. longicornis-are behaviourally-sophisticated and aggressive to occupy space and monopolize resources first [63][64][65] . The Yellow-Crazy Ant is one of the 100 notorious invasive species and one of the world's six harmful and widespread invasive ant species. The Longhorn Crazy Ant is also one of the fast-spreading species of tropical and subtropical natural and disturbed areas 65 . They share their position with the other globally notorious invasive ants-Solenopsis invicta (Red Imported Fire Ant), S. geminata (Tropical Fire Ant), Linepithema humile (Argentine Ant), Wasmannia auropunctata (Little Fire Ant), and Pheidole megacephala (African Big-Headed Ant) 15,64,65 . The Yellow-Crazy Ant seems to be the native of the Asia-Pacific and Indian Ocean region. Therefore, peninsular India, where this study took place, may be one of the native ranges of A. gracilipes. Yet, our study suggests that the Yellow-Crazy Ant can negatively affect the abundance, richness, and community of the native ants and abundance of certain species of invasive ants. Among the dominant invasive ant species, T. lanuginosum and M. floricola decreased with the abundance of A. gracilipes.
Some native and invasive ant species belonged to Pheidole, Tetramorium, Paratrechina, Tapinoma, and Monomorium collected in the present study are known to make super-colonies and adapt quickly to the disturbed environment, such as an urban environment. While that is so, the study also collected several tiny and sluggish subterranean ants known to live in the less-disturbed environments from the sacred groves of high urbanization. For instance, an examination of Strumigenys species collected in the present study (three species and 191 individuals) shows that their average proportional trap incidence was higher in the sacred groves of highly-urbanized sites than the moderately-and less-urbanized sites. These species are rare and endemic to the Western Ghats biodiversity hotspot. Their collection from the sacred groves of high urbanization sites reiterates that the native forest fragments of highly urbanized sites also support rare ants of the Western Ghats biodiversity hotspots and urbanization may not have a negative effect on the native ants.
In brief, our findings suggest that the sacred groves should be the hotspots of native ants. Although the disturbances due to urbanization and invasion of the invasive species can be an imminent threat for the native ant biodiversity, the healthy sacred groves are immune to such pressures. Although species diversity is unaffected by urbanization, the present study did not assess the effect of urbanization on the functional diversity of the ants. Throwing some lights on this will inform how urbanization might affect the forest functions. Although a plausible native of South Asia, the increase in the population of the Yellow-Crazy Ant, in the lights of its direct positive effects on the population of two other invasive ant species and direct negative effects on the abundance and diversity of the native ants, may be treated seriously. Future studies should also explore how the Yellow-Crazy Ant might affect the other elements of invertebrate biodiversity, such as beetles, collembolans, and epigeal flies.
Conserving the natural forests of any size should be our priority rather than replacing them with a manipulated artificial "forest". The sacred groves are the natural green islets, which support biodiversity, the cultural diversity, and the history of peninsular India, particularly the Western Ghats 28,29,31,39,40 . Urbanization is a significant driver of biodiversity and environmental change across latitudes and longitudes, and cities and city lifestyles are marauding the world. The cultural forests, such as sacred groves and church forests, because are conserved by the myths, beliefs, taboos, and religious reasons, can be the effective and sustainable global models of local biodiversity conservation. They would undoubtedly serve as the refuges for the urban wildlife and may be protected rather than replaced by the manipulated urban green islets or parks.