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Dietary nucleotides and yeast extract affect the composition and metabolic activities of the swine gut microbiota in a new in vitro fermentation model

Nucleotides (NT) are important compounds to many biological processes but data on the exact role of NT on the gut microbiota remain unclear. In this work we investigated the effects of dietary NT in a medium formulated to simulate the pig ileal chyme on gut microbiota composition and activity using a novel continuous in vitro intestinal fermentation models mimicking the proximal colon. Pig fecal microbiota was immobilized in gellan‐xanthan beads and used as a continuous inoculum in a novel intestinal model platform, operated with multiple parallel reactors to compare treatments on the exact same microbiota composition. Daily effluent samples were collected and analyzed to investigate the composition (qPCR, pyrosequencing) and metabolic activities (HPLC) of the microbiota.