{'fraction_non_alphanumeric': 0.026169706582077717, 'fraction_numerical': 0.0, 'mean_word_length': 5.341708542713568, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The influence of O‐GlcNAc in the motility of alveolar epithelial cancer cells

It's already well known that cancer cells increase glucose and glutamine uptake. The Hexosamine Biosynthetic Pathway (HBP) is highly dependent on both molecules and recent showed that cancer cells upregulate the HBP, including increased O‐GlcNAc levels, that we confirmed by immunohistochemistry. Two enzymes are responsible for the addition and removal of O‐GlcNAc (OGT and OGA, respectively). The transition of epithelial cells to mesenchymal cells (EMT) is crucial in metastasis of carcinoma cells by losing cellular polarity and increasing the cellular motility. In this work we used two approaches (Wound Assay and Colloidal Gold Assay) to investigate the role of O‐GlcNAc in cellular motility. Here, we showed that increase of O‐GlcNAc levels through OGA silencing or inhibition, increase the cell motility in alveolar epithelial cancer cells. Additionally, the reduction of O‐GlcNAc levels by OGT silencing showed a significant decrease the cell motility. In addition we showed increased levels of OGT and OGA during EMT induced by TGF‐β. These results suggest a role of O‐GlcNAcylation in the process of cell motility and may have implications in tumorigenesis. FAPERJ, CNPq