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Bone marrow mesenchymal stem cells-derived exosomal microRNA-150-3p promotes osteoblast proliferation and differentiation in osteoporosis.

BACKGROUND/AIM
At present, much more studies have focused on the role of microRNAs (miRNAs) in osteoporosis, but the more specific role of miR-150-3p in osteoporosis still needs full exploration. We aim to investigate the role of miR-150-3p in osteoporosis and explore the related mechanisms.


METHODS
Bone marrow mesenchymal stem cells (BMSCs) were cultured, from which exosomes were isolated. Osteoporosis models were established by ovariectomy and injected with transfected BMSCs exosomes. Bone formation markers in serum, histopathological changes and miR-150-3p, runt-related transcription factor 2 (Runx2) and Osterix expression, and bone status markers in femoral tissues were detected. Osteoblasts were isolated and co-cultured with the transfected BMSCs-derived exosomes. Osteoblast proliferation, cell differentiation and apoptosis, along with miR-150-3p, Runx2 and Osterix expression in osteoblasts were detected.


RESULTS
In vivo experiment demonstrated that miR-150-3p, Runx2 and Osterix expression were decreased while bone formation markers were decreased in osteoporosis. BMSCs exosomes attenuated osteoporosis which was further improved by up-regulated miR-150-3p in exosomes while impaired by down-regulated miR-150-3p in exosomes. In vitro experiments declared decreased miR-150-3p, Runx2 and Osterix expression, suppressed proliferation and encouraged apoptosis in osteoblasts in osteoporosis. BMSCs exosomes promoted osteoblast proliferation and differentiation and inhibited apoptosis, which was strengthened by raised exosomal miR-150-3p while disrupted by inhibited exosomal miR-150-3p.


CONCLUSION
Our study elucidates that exosomal miR-150-3p promotes osteoblast proliferation and differentiation in osteoporosis and provides a new clue for the treatment of patients with osteoporosis.