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Quantification of Dopamine in Portulaca oleracea Linn, by High-Performance Thin-Layer Chromatography

A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for quantification of dopamine in dried whole-plant powder of Portulaca oleracea Linn. The powder was extracted with 0.1 M HC1 and the extract was separated on aluminum HPTLC plates coated with silica gel 60 F254, with n-butanol-glacial acetic acid-distilled water 7.0:2.0:1.0 (v/v) as mobile phase. Detection and quantitation were performed by densitometry, with a deuterium lamp, at 280 nm. The response to dopamine reference standard was linear in the concentration range 45.0 to 110.0 μg cm−3. The validated method was used for quantitative analysis of dopamine in Portulaca oleracea Linn and can be used for routine quality-control analysis of whole-plant powder of Portulaca oleracea Linn.