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Separation and determination of phospholipids in biological samples by high-performance liquid chromatography.

An isocratic high-performance liquid chromatographic method is developed for the determination of phospholipids in biological samples using a muPorasil silica column and a mobile phase of acetonitrile-methanol-85% phosphoric acid (90:3:1, v/v/v) at a flow rate of 0.80 mL/min. The effluent is monitored by a UV detector at 203 nm. With the method reported in this paper, phosphatidylinostol, phosphatidylserine, phosphatidylethnolamine, and phosphatidylcholine in biological samples are separated and detected successfully. The method is simple, rapid, and has excellent precision.