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The control of blood pressure might be important in delaying progression of arterial aging in patients with type 2 diabetes mellitus

Objective Arterial stiffness, as assessed by the brachial-ankle pulse wave velocity (baPWV), is associated with arterial aging and has been consistently linked to cardiovascular disease. The factors involved in reducing the progression of arterial stiffness in patients with type 2 diabetes mellitus (DM) have not yet been fully established. Methods Of 478 patients who underwent two baPWV measurements (at baseline and 1 year later) at the Department of Internal Medicine, St Vincent’s Hospital, from November 2009 to June 2011, 341 subjects were enrolled in this study (male to female ratio =150:191; mean age, 62.1±7.7 years). The 341 subjects were over the age of 50 with type 2 DM, were diagnosed without peripheral artery disease, and 170 if the subjects (50%) had hypertension. Results baPWV at baseline increased in a linear manner along with age (β=22.8, t=10.855; P<0.0001, R2=0.258). After 1 year follow-up, the change in baPWV (ΔbaPWV) was variable (median 32.7 cm/s [approximate range, −557 to ∼745]). In multiple linear regression, the change in systolic blood pressure (β=7.142, 95% confidence interval =4.557–9.727; P<0.0001, R2=0.305) was associated with ΔbaPWV during follow-up. The change in glycated hemoglobin (HbA1c) and a glycemic control of keeping HbA1c levels below 7.0% were not associated with ΔbaPWV. Conclusion We found that the variation of blood pressure was associated with the progression of vascular aging of the large- to middle-sized arteries in patients with type 2 DM. Therefore, control of blood pressure might be important in reducing arterial aging or PWV in patients with type 2 DM.


Introduction
Pulse wave velocity (PWV), an indicator of arterial stiffness, is an independent predictor of cardiovascular morbidity and mortality. 1 The brachial-ankle PWV (baPWV) has been closely correlated with the carotid-femoral PWV, a noninvasive gold-standard marker for the assessment of aortic stiffness. 2 Measurement of baPWV is widely used in the general population because it has good reproducibility and does not require special skills from the technician. 3 PWV has a strong dependence on age and blood pressure (BP). 4 In a previous study, the annual rate of change in baPWV in healthy subjects was higher in the group with persistence of prehypertension than in the group with blood pressure in the normal range. Also, persistence of prehypertension accelerated structural stiffening of the large-to medium-sized arteries, and this type of aging may exert a synergistic effect on the acceleration of arterial stiffening. 2 Aging is associated with structural and functional changes in the vessel wall and elevated arterial stiffness. 5,6 The annual increase in aortic PWV before the age of

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Kim et al 50 years is approximately 100 mm/s and rises to more than 150 mm/s after the age 60 years. 7,8 Aging progression may be more pronounced in middle-aged and elderly subjects in healthy populations. 2 Several studies have reported that a higher baPWV is associated with more advanced latent and clinically atherosclerotic lesions of the arterial wall in hypertension (HBP). 9 Also, type 2 diabetes mellitus (DM) is a well-known cardiovascular risk factor often associated with and characterized by increased arterial stiffness. 10 However, in patients with type 2 DM, factors influencing progression of baPWV have not yet been fully established. In this study, we report on factors that may reduce the progression of arterial stiffness in patients with type 2 DM.

study population
We retrospectively enrolled 478 patients who underwent two baPWV measurements (ie, at baseline and again 1 year later) at the Medical Department of St Vincent's Hospital, Suwon, South Korea from November 2009 to June 2011. We excluded subjects with peripheral artery disease as diagnosed by an ankle-brachial index (ABI), subjects under the age of 50 years, and subjects without type 2 DM. Subjects (N=341) with type 2 DM over the age of 50 years (male to female ratio =150:191; mean age, 62.1±7.7 years) were enrolled in this study. Controlled DM was defined as achieving and keeping glycated hemoglobin (HbA 1c ) levels at 7.0%. There was no industry involvement in the design, conduct, or analysis of the study. The Ethics Committee of St Vincent's Hospital approved the use of clinical data for this study, and all patients provided written informed consent.

PWV measurements
Bilateral brachial and ankle BPs and arterial pulse waves were simultaneously measured using an automatic waveform analyzer (VP-1000; Omron Healthcare, Kyoto, Japan).
Individuals were examined in the supine position after at least 5 minutes bed rest. Pneumatic cuffs were wrapped around both upper arms and ankles, and connected to a plethysmographic sensor to determine the volume pulse waveform. Bilateral brachial and posterior tibial artery pressure waveforms were stored for a 10 second sample time with automatic gain analysis and quality adjustment. baPWV was recorded and calculated using the formula in which L1 and L2 were defined as the distance from the heart to the ankle, and the distance from the heart to the brachium, respectively, and T was defined as the transit time between the brachial and posterior tibial artery waveforms. The average of baPWV measurements taken from the left and right sides were used for the analysis. The change in baPWV during the study period (ΔbaPWV) was calculated as follows: value at the second examination − value at the baseline examination.
The ABI was defined as the bilateral ratio of the systolic BP of the posterior tibial artery to that of the brachial artery.

Clinical and biochemical assessment
Blood specimens were collected after a 12-to 14-hour fast (8 pm to 9.30 am) to reduce the influence of circadian variation. Total cholesterol and triglyceride concentrations were measured using standard enzyme methods. Highdensity lipoprotein cholesterol was measured after precipitation of very-low-density lipoproteins and low-density lipoprotein with phosphotungstic acid, and low-density lipoprotein was calculated using the Friedewald formula. Fasting glucose levels were enzymatically determined by the hexokinase method. 11 A blood sample from every patient was drawn and centrifuged within 30 minutes; serum samples were stored at −80°C, and high sensitivity C-reactive protein was determined using an immunoturbidity assay system (Liatest; Stago, Asnières-sur-Seine, France), with an interassay variability coefficient of variation of 6.25%. 12 Blood pressure measurement Blood pressure was measured with a mercury sphygmomanometer (YAMASU, Saitama, Japan), (first and fifth phases of the Koroktoff sounds taken as systolic BP and diastolic BP, respectively) after subjects had rested for 5 minutes in the sitting position. An average of three measurements was used in the analysis. Systemic hypertension was defined as systolic pressure 140 mmHg and/or diastolic pressure 90 mmHg, based on more than three measurements or current use of antihypertensive drugs. BP measurements were performed blinded to baPWV measurements.

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Arterial aging and blood pressure in diabetes patients University, Vienna, Austria). Continuous values are expressed as the means ± standard deviation (SD). The means were compared by the Student's t-test for independent samples with the assumption of equal variance, and also the paired t-test for dependent samples. Analysis of categorical data was carried out with the χ 2 test or Fischer's exact test, where appropriate. The limit of statistical significance was set at P0.05. Variables yielding P-values 0.05 by univariate analysis and variables considered clinically relevant, were entered into the multivariate analysis to estimate the independent association of each covariate with the dependent variable. Multivariate analysis consisted of linear regression analysis for independent variables related to the change in ΔbaPWV.

Results
Baseline characteristics of the 341 patients with type 2 DM over the age of 50 at the first visit are shown in Table 1. All patients without peripheral artery disease were diagnosed by ABI. At the first visit, the mean age of study subjects was 62.1±7.7 years, and 150 (44%) were men. baPWV at baseline increased in a linear manner with age (β=22.8, t=10.855; P0.001, r=0.508) (Figure 1). All patients underwent baPWV measurements twice (ie, at baseline and 1 year later; mean, 351±60 days). After 1-year follow-up, ΔbaPWV was variable (median 32.7 m/s [range, −557 to ∼745]). Table 2 shows the correlation between ΔbaPWV and multiple risk factors.  (Table 3).

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Kim et al of elastic fibers, the accumulation of advanced glycation end-products on collagen and elastin fibers, and endothelial dysfunction. 18 In a previous longitudinal study, uncontrolled BP and increased heart rate (HR) could have accelerated agerelated progression of arterial stiffness using aortic PWV as a measure over a 6-year period in treated hypertensive subjects. 19 In another study, persistence of prehypertension, not HR, was associated with progressive arterial stiffness using baPWV as a measure. 2 However, in treated type 2 DM patients, the role of risk factors on the progression of arterial stiffness using baPWV as a measure has not yet been evaluated. This study showed that changes in systolic BP could have accelerated the progression of arterial damage, by using baPWV as a measure in treated type 2 DM patients.
Some limitations to our study deserve mention. First, baPWV is not considered the gold standard for assessing arterial stiffness. 20 However, the validity and reproducibility of baPWV measurements are very high, and this method seems to be an acceptable marker reflecting vascular damage. baPWV measured by this simple, noninvasive method is suitable for screening vascular damage. Second, a limitation of the present study is that the effect of different BP lowering therapies was not considered. However, most patients (over 90%) were taking more than one BP-lowering drug, including angiotensin-converting enzyme (ACE) inhibitors, or angiotensin II receptor blockers (ARBs).
In conclusion, the present longitudinal study shows that ΔSBP was associated with the progression of vascular aging of the large-to middle-sized arteries in patients with type 2 DM. Therefore, BP control might be important factor in reducing arterial aging or PWV in patients with type 2 DM.

Disclosure
The authors report no conflicts of interest in this work.

Discussion
The present study is a longitudinal study to evaluate the determinants of the progression of arterial stiffness over a period of 1 year. This study shows that the progression of baPWV in patients with type 2 DM over the age of 50 was correlated with a change in systolic BP at follow-up. ΔSBP was identified as being responsible for accelerated progression of baPWV in patients with type 2 DM. ΔHbA 1c , and a glycemic control of keeping HbA 1c levels below 7.0%, were not associated with ΔbaPWV. Arterial stiffness has been shown to be an independent predictor of cardiovascular mortality, and greatly increases with age. 13 Arterial stiffness is increased when elastic properties of the arterial wall are reduced. 14 Aging is a process that causes structural and functional modifications in the vasculature, resulting in decreased arterial compliance and ability of arteries to withstand strain. 7 Several cross-sectional studies have shown that in addition to age, several other environmental and genetic factors can influence arterial stiffness. [15][16][17] The striking features of arterial stiffening are an increased density of collagen fibers, decreased density