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Anti-leukemic activity of Murraya paniculata L. Jack Leaf Extract and Cell Cycle Arrest

We evaluated the cell viability activity of methanolic extract of Murraya paniculata leaf (MEMPL) on leukemia cells. MEMPL significantly reduced the cell viability in a time and concentration dependent manner in both the acute myeloid and acute lymphoid cells. Several apoptosis indicators were found in cancer cells, including chromatin condensation, nuclear fragmentation according to the morphological study. These findings suggest that MEMPL exhibited anticancer activity in leukemic cell lines. Flow cytometric analysis found a significant number of cells in the early and late stages of apoptosis. MMP shift assay revealed a significant change in mitochondrial membrane potential in MEMPL-treated leukemic cells. The cells were mostly in the G0/G1 phase of the cell cycle when they were arrested. These findings reveal that MEMPL showed anticancer activity in leukemic cell lines.