{'fraction_non_alphanumeric': 0.0195971692977681, 'fraction_numerical': 0.024496461622210124, 'mean_word_length': 5.188552188552189, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The PxIxIT motif of the RCAN3 Inhibits angiogenesis and tumor progression in Triple Negative Breast Cancer in Immunocompetent Mice.

RCAN proteins are endogenous regulators of the calcineurin- cytosolic nuclear factor of activated T-cells (CN- NFATc) pathway that bind CN through similar conserved motifs PxIxIT and LxVP of the NFATc family. It has been reported that RCAN1 and RCAN3 protein levels correlate with overall survival of breast cancer patients. We have additionally provided supporting results about RCAN3 role on cancer showing that overexpression of the native PxIxIT sequence of RCAN3-derived R3 peptide (PSVVVH, EGFP-R3178-210) dramatically inhibits tumor growth and tumor angiogenesis in an orthotopic mouse model of Triple Negative breast cancer (TNBC). On the other hand, RCAN3 protein and its derived peptide EGFP-R3 178-210 bind to CN and inhibit NFAT-mediated cytokine gene expression without affecting CN phosphatase activity suggesting that RCAN3 and EGFP-R3 178-210 peptide have tumor suppressor and immunosuppressant activity. Due to the known relationship between tumor development and immune system, as well as the relevance of CN-NFATc in the regulation of the immune system, we decided to study the effect of EGFP-R3 178-210 peptide in a syngeneic TNBC model, in order to ensure that the role of RCAN3 as immunosuppressant do not override its tumor suppressor activity. Our results evidence that EGFP-R3 178-210 peptide displays an inhibitory potential on tumor growth and tumor angiogenesis similar to those obtained in the previous orthotopic TNBC model. These results highlight the importance of the RCAN3 peptide as a tumor suppressor protein and totally complement our previous results, indicating that this antitumor activity role is maintained in the presence of a complete functional immune system.