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Genetic evidence that protein synthesis is required for the circadia clock of neurospora.

Small doses of cycloheximide given at intervals (pulses) cause phase shifts of the circadian clock of Neurospora. The effects of this drug on the clock are mediated through its inhibition of protein synthesis, since two cycloheximide-resistant mutants whose 80S ribosomes are resistant to cycloheximide showed no phase shift after exposure to the durg.