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Self‐splicing of the Tetrahymena group I ribozyme without conserved base‐triples

Group I introns share a conserved core region consisting of two domains, P8‐P3‐P7 and P4‐P6, joined by four base‐triples. We showed previously that the T4 td intron can perform phosphoester transfer reactions at two splice sites in the absence of both P4‐P6 and the conserved base‐triples, whereas it is barely able to perform the intact splicing reaction due to the difficulty of conducting the sequential reactions.