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[Prediction and identification of linear B-cell epitopes in major group 3 allergen derived from Dermatophagoides farina].

OBJECTIVE
To predict and identify the linear B-cell epitopes in the major group 3 allergen derived from Dermatophagoides farina (Der f 3).


METHODS
The linear B-cell epitopes of Der f 3 allergen were analyzed based on the physicochemical properties of amino acids including antigenicity, surface accessibility, flexibility, hydrophilicity, beta-turn by online bioinformatics softwares. The eight predicted peptides of linear B-cell epitopes were artificially synthesized and incubated with three aller gic serum pools (4 serum samples in each), which were consisted of total 12 serum samples from the allergic individuals, and the strong positive epitopes were selected.


RESULTS
Eight B-cell epitopes from Der f 3 were predicted successfully. Five of eight B-cell epitopes were identified with strong IgE-binding abilities followed by specific IgE assay. The amino acid sequences of them were following: 33KAKAGDCP40, 86HASGGEKIQVAEIYQHENYDSMTID110, 118LKTPMTLDQTNAKPVPLPPQGSDVKVG144, 156QEGSYSLP163 and 199DVANGGVDSCQGDSGGPVVD218.


CONCLUSIONS
Five linear B-cell epitopes of Der f 3 allergen have been identified successfully. This result might provide a basis of the diagnosis and treatment for asthma.