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Separation cloning and application of rice photoperiod-sensitive male-sterile gene pms3

The invention belongs to the technical field of plant gene engineering, and concretely relates to the technical field of rice gene engineering. A rice photoperiod-sensitive male-sterile gene pms3 for coding long-chain RNA is obtained through separating by a cloning map-based method and can regulate and control the sterility of rice pollen. The nucleotide sequence of the pms3 gene is represented by SEQ ID NO:2. The nucleotide sequence of the allele of the pms3 gene is represented by SEQ ID NO:3, and there is a C-to-G allele mutation at the 790bp site of the sequence. A molecule marker for breeding the rice photoperiod-sensitive male-sterile gene is also obtained in the invention, the nucleotide sequence of the molecule marker is represented by SEQ ID NO:1, and there is a base mutation at the 1711bp site of the sequence. The rice pollen sterility control gene pms3, its allele and the molecule marker for breeding the rice photoperiod-sensitive male-sterile line obtained in the invention can be widely applied to the breeding and the heredity improvement of rice, especially the generation of a new rice sterile line and the photoperiod-sensitive rice sterile line.