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Origin of serum alpha-amylase isoenzymes from healthy adult rats.

In connection with common use of rats in experimental testing drug toxicity in the liver or pancreas, we checked the electrophoretic patterns of amylase isoenzymes extracted from the liver, pancreas, parotis and serum of healthy adult rats. Separation of isoenzymes was made by polyacrylamide gel electrophoresis. The results indicate the salivary glands and liver as sources of serum alpha-amylase.