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K, Fya, and Jka phenotyping of donor RBCs on microplates

BACKGROUND: In many cases, the search for compatible blood for patients with clinically significant RBC alloantibodies is difficult and time‐consuming. To date, it has been considered necessary only to phenotype the blood donors for ABO group and D. There has been long experience with automated routine analysis (ABO, C, c, D, E, and e typing and RBC antibody screening), using robotic dispensers and computerized interpretation of microplate results. The purpose of this study was to explore the possibilities of also phenotyping for K, Fya, and Jka, as antibodies directed against these antigens (together with Rh antigens) are the most common clinically significant alloantibodies in the Swedish population.