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A new organotypic model containing dermal‐type macrophages

Abstract:  Human skin equivalents (SEs) are popular three‐dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti‐inflammatory dermal macrophages has been performed. Here, we show that monocyte‐derived dermal‐type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC‐SIGN/CD209 and HLA‐DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti‐inflammatory cytokine IL‐10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage–fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages.