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Cell culture studies in 19 cases of refractory aneamia: comparison of clinical data with in vivo erythrokinetic studies.

A total of 19 cases of chronic refractory anaemia underwent simultaneous in vivo erythrokinetic study and in vitro bone marrow culture. They were followed up clinically for at least 2 years. Good correlation has been found between erythrokinetic data (simultaneous quantitative and qualitative disorders of erythroblastic proliferation in preleukaemic states; pure qualitative disorders in primary sideroblastic anaemia) and the results of culture of granulocyte precursors in the bone marrow (small number of colonies, reduced size of colonies in preleukaemic states; normal number and growth in non malignant refractory anaemia). It would seem thus that both examinations are of practical interest in clinical haematology, making it possible to foresee the malignant evolution of some refractory anaemias.