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In situ Structure of Viral RNA by Cryo Electron Tomography with Volta Phase Plate, Energy Filtering and Direct Electron Counting

The most significant limiting factor of biology electron microscopy is that only low electron dose (about 25 to 100 electrons/Å depending on the sample and the target resolution) is available for imaging prior to sample damage. As a result, cryo electron microscopy (cryoEM) images of biological complexes are characterized by poor contrast and low signal/noise ratio. Single-particle cryoEM relies on the power of averaging low-contrast, high-resolution images of as many as one million purified particles of the same homogeneous complex to overcome this low contrast problem to obtain structures suitable for functional interpretation. This approach was first demonstrated at near atomic resolution for proteins in an icosahedral virus [1] and now has become a method of choice for structure determination of protein complexes in general. For non-homogeneous structures, such as nucleic acid molecules inside many viruses, however, cryo electron tomography (cryoET) must be used. Without the power of averaging, resolving nucleic acid structures within viruses by cryoET has not been possible.