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Nonselectivity of Rimler-Shotts Medium for Aeromonas hydrophila in Estuarine Environments

Thirty presumptive Aeromonas hydrophila isolates were collected from estuarine sources using Rimler-Shotts media. Many of the isolates, especially the strains from a low-salinity site, were also identified as A. hydrophila using the API 20E system and two differential techniques devised for the identification of A. hydrophila. All of the isolates, however, had deoxyribonucleic acid base compositions with guanine-plus-cytosine ratios between 40 and 49 mol%, which excludes these strains from the genus Aeromonas.