{'fraction_non_alphanumeric': 0.04426559356136821, 'fraction_numerical': 0.023474178403755867, 'mean_word_length': 5.543859649122807, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Effects of interleukin 2 receptor b chain (P75)‐specific monoclonal antibody on the generation of cytotoxic T lymphocytes and suppressor T cells in mixid lymphocyte culture

The interaction of interleukin 2 (IL‐2) with its receptor (IL‐2R) plays an essential role in the proliferation and differentiation of T cells. The IL‐2R β‐chain is considered to function directly in the intracellular signal transduction. In this study, we investigated using a newly established IL‐2R β‐chain‐specific monoclonal antibody (MAb) (TU‐25) and an IL‐2R α‐chain‐specific MAb (H‐31). The IL‐2‐induced proliferation of concanavalin blasts and the mixed lymphocyte reaction (MLR) were suppressed by TU‐25 in combination with H‐31. This combination had a greater suppressive effect than each of them alone. The generation of cytotoxic T lymphocytes (CTL) using a cell‐mediated lympholysis (CML) assay, was not inhibited by TU‐25 alone. TU‐25 in combination with H‐31 suppressed the generation of CTL completely in this assay even if recombinant IL‐2 (rIL‐2) was added. Although the CTL generation was inhibited, cells that suppressed a fresh MLR were preserved. Our study suggests that the combination of TU‐25 with H‐31 completely blocks the functional high‐affinity binding site of IL‐2 but does not inhibit the generation of suppressor cells. This may lead to immunosuppressive therapy using an IL‐2R 13‐chain‐specific MAb in combination with an IL‐2R α‐chain‐specific MAb in clinical organ transplantation.