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Immunoreactive calcium‐binding protein (calbindin‐D28k) in interneurons and trigeminothalamic neurons of the rat nucleus caudalis localized with peroxidase and immunogold methods

Calbindin‐D28k is a highly abundant protein found in neurons in selected brain regions, including cells in sensory system of the brainstem. Because of its capacity to bind cytosolic ca++, calbindin‐D28k is thought to contribute to the regulation of compartmental Ca++ concentration in neurons. In this study of the rat spinal trigeminal nucleus, calbindin‐D28k was localized with immunoperoxidase and immunogold methods. Results showed that immunoreactive calbindin‐D28k neurons were widely distributed to all region of the nucleus, but were particularly numerous in the substantia gelatinosa. Some trigeminothalamic neurons that were identified by retrograde labeling of conjugated wheat‐ germ agglutinin with horseadish perosidase also contained calbindin‐D28k immunoreactivity. Most of the calbindin‐D28k labeling was found in cell bodies and dendrites. Axon terminal were rarely stainded. More discreate labeling with a gold conjugated second antibody showed that the predominant site of calbindin‐D28k was the matrix of the cytoplasm. Gold label was also heavily associated with euchromatin within nuclei.