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A functional regulatory variant of MYH3 influences muscle fiber-type composition and intramuscular fat content in pigs

Muscle development and lipid accumulation in muscle critically affect meat quality of livestock. However, the genetic factors underlying myofiber-type specification and intramuscular fat (IMF) accumulation remain to be elucidated. Using two independent intercrosses between Western commercial breeds and Korean native pigs (KNPs) and a joint linkage-linkage disequilibrium analysis, we identified a 488.1-kb region on porcine chromosome 12 that affects both reddish meat color (a*) and IMF. In this critical region, only the MYH3 gene, encoding myosin heavy chain 3, was found to be preferentially overexpressed in the skeletal muscle of KNPs. Subsequently, MYH3-transgenic mice demonstrated that this gene controls both myofiber-type specification and adipogenesis in skeletal muscle. We discovered a structural variant in the promotor/regulatory region of MYH3 for which Q allele carriers exhibited significantly higher values of a* and IMF than q allele carriers. Furthermore, chromatin immunoprecipitation and cotransfection assays showed that the structural variant in the 5′-flanking region of MYH3 abrogated the binding of the myogenic regulatory factors (MYF5, MYOD, MYOG, and MRF4). The allele distribution of MYH3 among pig populations worldwide indicated that the MYH3 Q allele is of Asian origin and likely predates domestication. In conclusion, we identified a functional regulatory sequence variant in porcine MYH3 that provides novel insights into the genetic basis of the regulation of myofiber type ratios and associated changes in IMF in pigs. The MYH3 variant can play an important role in improving pork quality in current breeding programs.


Introduction
Despite the remarkable progress of studies using high-throughput genome technologies combined with genome-wide linkage and association in various organisms, elucidating the genetic architecture of complex quantitative traits remains a key challenge of modern biology [1]. In this respect, the phenotypic and genetic diversity among breeds of domestic animals provides an excellent opportunity to investigate the relationship between phenotypic and genotypic variations [2,3]. Despite their retarded growth, Korean native black pigs (KNPs) found on Jeju Island are renowned for their meat quality characteristics, such as a reddish meat color (a � ) and a high degree of marbling (i.e., intramuscular fat, IMF) compared with the traits of the Western commercial pig breeds such as Landrace (Fig 1A and 1B) [4]. The clear a � of KNPs is mainly due to the large amount of slow/type1/oxidative myofibers in the muscle tissue ( Fig 1C  and 1D), and the high degree of marbling is associated with the excess accumulation of IMF ( Fig 1E). The variation in muscle fiber composition and IMF content is complex; highly interrelated phenomena are dependent on multiple genetic components and, environmental conditions together with a host of various cellular signals and hormones involved in myogenesis and adipogenesis [5,6]. Therefore, it is expected that muscle fiber-and IMF-related traits are remarkably complex quantitative traits, of critical economic importance, but the underlying genetic basis is largely unknown. Here, linkage and association analyses were conducted to dissect the genetic architecture of a � and IMF traits in pork, and a major QTL on chromosome 12 was identified to be significantly associated with the two traits. The aim of this study was to elucidate and characterize the genetic determinant underlying the major QTL.

Identification of the porcine MYH3 gene as a putative quantitative trait gene (QTG) for a � and IMF content
To investigate the genetic basis underlying a � and IMF in the longissimus dorsi muscle, we generated a large intercross between Landrace pigs and KNPs with 1,105 F 2 progeny (LK cross) [7] and identified a quantitative trait locus (QTL) located on pig chromosome 12 (SSC12) that had a substantial effect on both a � and IMF based on genome-wide linkage analysis [8]. Previous studies reported that the QTL on SSC12 contains a cluster of genes encoding the myosin heavy chains (MYHs) which are strongly associated with a � and IMF [9,10]. Here, we genotyped the entire LK cross using the Illumina PorcineSNP60K BeadChip platform [11,12]. A genome-wide association study (GWAS) revealed a major locus for the two traits [a � : 26.6% phenotypic variance explained by the SNP (% Var SNP ), P-value = 1.5×10 −70 ; IMF: 24.2% Var SNP , P-value = 1.1×10 −88 ] at the SSC12 region harboring the MYH gene cluster (S1A and S1B Fig). The position of the most significantly associated marker (rs81437379) for the two traits was 54,956,054 (NCBI Sus scrofa version 11.1). Using linkage analysis, instead of an association study, we could also replicate the major QTL for the two traits at rs81437379 (S2A Fig; a � : 20.5% Var QTL , P-value = 1.02×10 −49 ; IMF: 29.4% Var QTL , P-value = 3.2×10 −74 ).
To refine the identified QTL on SSC12 in the LK cross, we conducted a joint linkage and linkage disequilibrium (LALD) analysis of a � and IMF using DualPHASE software [13]. The test statistics of the LALD mapping for a � (P-value = 3.47×10 −103 ) and IMF (P-value = 7.35×10 −136 ) were maximized at the 718.4-kb QTL region (i.e., 12 To confirm the QTL signals identified in the LK cross, we established another independent intercross between Duroc pigs and KNPs with 381 F 2 offspring (DK cross). All of the individuals in this cohort were also genotyped using the Illumina PorcineSNP60K BeadChip in a manner similar to the genotyping of the LK cross. The additional GWAS and linkage analysis replicated the finding of the highly significant association and linkage signals for a � (P-value for association = 7.2×10 −11 ; P-value for linkage = 1.2×10 −16 ) and IMF (P-value for association = 4.3×10 −13 ; P-value for linkage = 3.2×10 −19 ) at the same location (rs81437379) on SSC12 as detected in the LK cross (S1C and S1D Fig; S2B Fig). Furthermore, the LALD mapping using  (right). Note the pronounced difference in reddish meat color (a � ) as well as marbling (i.e., intramuscular fat, IMF). (C) Myosin ATPase histochemistry after preincubation at pH 4.6 of the longissimus dorsi muscle from the Landrace (left) and KNP (right). Red arrows indicate type1 (slow/ oxidative) fiber; yellow arrow heads indicate type2A fiber (fast/oxido-glycolytic); gray arrowheads indicate type2B fiber (fast/glycolytic). Scale bar = 200 μm. (D) Boxplot with individual raw a � values of Landrace (n = 43) and KNP (n = 21) (E) Boxplot with individual raw IMF contents of Landrace (n = 43) and KNP (n = 21).
https://doi.org/10.1371/journal.pgen.1008279.g001 According to the NCBI Sus scrofa 11.1 annotation, the critical interval contained eleven protein coding genes, including LOC100736982, LOC110255887, MYH4, MYH1, MYH2, MYH3, LOC100517855, ADPRM, TMEM220, LOC110255888 and PIRT ( Fig 2C). Based on comparative sequence and phylogenetic analyses, LOC100736982 and LOC110255887 were identified as MYH13 and MYH8, respectively (S1 Table and  In both the longissimus and quadriceps muscles, quantitative reverse transcriptase-PCR (qRT-PCR) detected a highly significant difference in MYH3 transcript level abundance between KNPs and Landrace pigs, with this gene being transcribed approximately 7-12-fold more actively in KNPs than in Landrace pigs ( Fig 2D). The results of Western blotting analysis using proteins prepared from the skeletal muscle samples confirmed the differential expression of MYH3 between the two pig breeds (Fig 2E). We did not detect any significant differences in transcription levels in any of the other genes located within this critical interval.

Phenotypic changes induced by ectopic expression of porcine MYH3 in transgenic (TG) mice mimic the KNP phenotype
To evaluate the role of MYH3 in muscle fiber composition and lipogenesis in skeletal muscle, we generated TG mice overexpressing porcine MYH3 (S6A and S6B Fig). No significant body weight difference was observed between TG mice and wild-type (WT) mice (S6C Fig). From a morphological aspect, the hindlimb of the TG-mouse strain number 24, which exhibited the highest MYH3 expression among the four TG-mouse strains (S6A and S6B Fig), showed a recognizable reddish color that is characteristic of slow/type1/oxidative muscle fiber, while the counterpart of the WT-mouse strain was paler in appearance, strongly suggesting the influence of MYH3 on muscle fiber-type composition ( Fig 3A). Accordingly, myosin ATPase staining analysis revealed a greater presence of slow/type1/oxidative fibers in the quadriceps muscles of TG mice, whereas the quadriceps muscles of the WT mice mainly contained fast-type fibers (fast/type2A/oxido-glycolytic and fast/type2B/glycolytic) (Fig 3B). In particular, a significant increase in the area of the slow type muscle fiber was detected (P-value = 0.0004) in TG mice compared to that of the WT mice, whereas, no significant difference (P-value = 0.93) in the area of the fast-type fibers was found (S6D Fig).
The TG-mice overexpressing MYH3 also showed enhanced levels of myoglobin, troponin I (Tn I), and mitochondrial oxidative cytochrome c enzyme (Cyt C), all of which are critical features of slow/type1/oxidative muscle fibers (Fig 3C). Messenger-RNA expression of Myh7, which is a molecular marker for slow/type1/oxidative fiber [14], was higher in the TG mice QTL did not show any sign of additional QTL on SSC12 (yellow and gray dotted lines). (B) LALD mapping results on SSC12 for a � and IMF from the DK cross. The vertical dotted lines for each cross were estimated by the LOD-drop method (S3 Fig). (C) Blue (LK cross, 3-LOD drop support interval) and green (DK cross, 2-LOD drop support interval) boxes indicate cross-specific LOD-drop support intervals. Maximum test statistics for each cross were obtained at the region colocalized in the 488.1-kb critical shared region represented by the black double headed arrow (12: 55,073,130-55,561,243). Eleven NCBI protein coding genes are located within the 488.1-kb critical interval associated with a � and IMF. Gene names in parentheses were annotated by this study (S1 Table and  than in the WT mice (P-value < 0.05). However, the three molecular markers for fast-type muscle fibers (i.e., Myh1, Myh2, and Myh4 [14]), showed no significant differential mRNA expression between the two strains of mice ( Fig 3D). Western blotting analysis of Myh7 and Myh4 also demonstrated the same expression pattern as their transcripts (Fig 3D). The results of immunohistochemical analysis showed a high degree of colocalization of porcine MYH3 and slow Myh7 in the TG-mice, while the opposite localization of porcine MYH3 and fast Myh4 was detected ( Fig 3E). Moreover, qRT-PCR analysis revealed strongly increased gene expression of slow/type1/oxidative fiber associated genes (Myoglobin, Tnnt1, Tnni1, and Tnnc1). However, no significant expression difference in fast-type muscle fiber-associated genes (Aldoa, Pvalb, Tnnt3, Tnni2, and Tnnc2) was observed between TG and WT mice (S6E Fig). Combined, these results clearly indicate that overexpression of MYH3 influences the myofiber composition in the skeletal muscle of the TG mice.
The way in which MYH3 functionally influences the expression of adipogenesis-related genes is still unknown. Nevertheless, we selected eight genes actively involved in adipogenesis (i.e., CD36, Lpl, Fabp4, Fto, Ppargc1α, Adipoq, Pparγ, and C/ebpα) to test whether the overexpression of the MYH3 gene influences the expression of genes known to be involved in the adipogenesis pathway in skeletal muscle [15][16][17]. The eight adipogenesis-related genes displayed significantly higher mRNA expression in TG mice compared to WT mice ( Fig 3F). Furthermore, the ingenuity pathway analysis (IPA) was used to infer a molecular interaction network of adipogenesis ( Fig 3G). The IPA also identified the adipogenesis pathway as the top-ranked canonical pathway with strong statistical support (P-value = 1.02×10 −7 ). These results suggest that the overexpression of MYH3 regulates the coordinated expression of genes involved in adipogenesis in the skeletal muscle tissues of the TG mice partially due to increased IMF resulting from the more abundant myofiber type I. MYH3 overexpression in quadriceps skeletal muscles also enhanced levels of intramyocellular triacylglycerol (TAG) and free fatty acids (FFAs) (Fig 3H). These data further support MYH3 as the most likely QTG responsible for the regulation of myofiber type ratios and the associated changes in adipogenesis in the skeletal muscle of both mice and pigs.

Sequencing F 1 sire chromosomes, marker-assisted segregation analysis (MASA), and in silico functional annotation detect putative functional sequence variants (FSVs) of MYH3 affecting a � and IMF
To identify FSVs in the 488.1-kb critical region that affect the porcine MYH3 gene (S3 Fig), we first sequenced genomic DNA samples from the F 1 sires of LK (n = 18) and DK (n = 6) crosses together with the parental animals using a massively parallel sequencing technology [i.e., Landrace (n = 17) and KNP (n = 19) for LK cross; Duroc (n = 9) and KNP (n = 5) for DK cross]. The criteria to detect putative FSVs among the identified DNA sequence variants (DSVs) within the 488.1-kb critical interval were as follows: 1) the FSV has to be biallelic since the effect of the founder haplotypes followed a bimodal distribution (S5 Fig); 2) it should be segregating in both LK and DK crosses; and 3) it should affect the binding capacity of regulatory sequence motifs i.e., transcription factor binding sites such as the enhancer, silencer and promoter in the noncoding region, because we detected a clear differential MYH3 expression between the two parental breeds (Fig 2D and 2E). In this 488.1-kb critical region, we detected 7,606 DSVs from the LK cross and 5,211 DSVs from the DK cross. Using 12 (LK cross) and 5 (DK cross) informative F 1 sire families, we performed MASA to test whether the identified variants in the MYH3 region fulfilled the biallelic QTL assumption [18,19]. The results indicated that eight sires were heterozygous for the QTL genotype (Q/q) and four were homozygous (either Q/Q or q/q) (S2 Table and S7A Fig). Therefore, the genotype of the putative FSV should be homozygous in the four nonsegregating F 1 sires and heterozygous in the eight segregating F 1 sires in the LK cross. Application of this criterion to the 7,606 DSVs sorted out 548 putative FSVs in the 488.1-kb critical region in the LK cross. Likewise, this approach was applied to the F 1 sires from the DK cross. We detected 5,211 DSVs in the critical region from the 5 DK F 1 sires (S2 Table and  To investigate whether these 547 variants are located in putative regulatory motifs in the noncoding regions of the 488.1-kb critical interval, we computationally predicted the motifs in the entire critical interval using the MEME suite [20]. Among the 547 variants, ninety putative FSVs were detected within the motifs predicted by the MEME suite (S3 Table). Subsequently, we investigated whether the ninety predicted motifs could be annotated using TRAP, JASPAR and PROMO programs [21][22][23]. A total of twenty-two predicted motifs were annotated as transcription factor binding sites based on the in silico analysis using the three programs (S3 Table). Notably, a motif located in the 2-kb of the 5 0 promoter/regulatory region of the MYH3 gene was predicted as the binding site for all four known myogenic regulatory factors (MRFs) (i.e., MYOD, MYOG, MYF5 and MRF4). The Sanger sequencing analysis of the 2-kb promoter region of the MYH3 gene using the parental animals revealed a 6-bp deletion (XM_013981330.2:g.−1805_−1810del, chr12: 55,373,707) at the motif in the KNPs (Fig 4A). The 6-bp deletion variant, XM_013981330.2:g.−1805_−1810del, fulfilled the criteria of being a biallelic and overlapping FSV between the two cohorts. Moreover, this 6-bp deletion variant is located in the MYH3 promoter at the position of the overlapping binding motifs for the four MRFs ( Fig 4A). Thus, the XM_013981330.2:g.−1805_−1810del is expected to affect the binding of these MRFs.

Bayesian fine-mapping of the 488.1-kb critical region characterizes XM_013981330.2:g.−1805_−1810del as a candidate causal FSV
To investigate whether the XM_013981330.2:g.−1805_−1810del obtained from the MASA and in silico functional annotation can be regarded as a candidate causal FSV, we applied Bayesian fine-mapping approaches using CAVIAR and eCAVIAR programs [24][25][26]. Prior to the finemapping analyses, we reconducted the PorcineSNP60K BeadChip-based GWAS including the XM_013981330.2:g.−1805_−1810del to obtain summary association statistics of the 488.1 critical region [27]. XM_013981330.2:g.−1805_−1810del was revealed as the top-ranked variants for a � and IMF in the 488.1-kb critical region (Table 1). In the Bayesian fine-mapping using the CAVIAR program, we established a 99% credible set of variants within which the candidate causal FSV(s) for a � and IMF are most likely to be included. The CAVIAR results revealed that the 99% credible set included only XM_013981330.2:g.−1805_−1810del with an extremely high posterior probability of being a candidate causal variant (S4 Table). Additionally, we performed bivariate Bayesian fine-mapping using the eCAVIAR program to provide evidence for whether XM_013981330.2:g.−1805_−1810del has a pleiotropic effect on a � and IMF. The combined likelihood posterior probability from eCAVIAR provided evidence that the MYH3 6-bp deletion variant can be considered as a pleiotropic variant for both a � and IMF (S4 Table).
These results provide information on the contrast between this particular 6-bp deletion variant and other variants used for GRAMMAR based GWAS. The regional P-value plot showed that it is extremely difficult to distinguish which variant should be considered as a candidate causal variant from the GRAMMAR based GWAS; there are several variants that can be regarded as candidate causal variants based on their P-value (S10 Fig). In this case, CAVIAR- based fine-mapping demonstrated a power to discriminate candidate causal variant(s) from the other variants in the GWAS.
The effect of this candidate causal variant on the phenotypes is shown in Table 1. The results show that the KNP originated Q allele is favorably associated with meat quality-related traits, whereas the Landrace originated q allele is positively associated with growth-related traits. The Q allele is also favorably associated with mRNA expression levels of the porcine MYH3 gene in the longissimus dorsi muscle (Fig 4C). The genic action is mostly additive for all traits examined.

Functional characterizations reinforce the critical importance of the MYH3 6-bp deletion variant
Subsequently, we analyzed the effect of the candidate functional variant of MYH3 on transcription using transient transfection assays with luciferase reporter constructs containing either the Landrace (q) or KNP (Q) sequence fragments in porcine fibroblast cells [28,29]. Compared with the promoterless construct, both the constructs containing the Q and the q sequence fragments increased luciferase reporter activity: the reporter activity increased~3.7-fold for the Q construct and~1.6-fold for the q construct. The reporter gene activity was consistently higher for the Q constructs than for the q constructs ( Fig 5A). In addition, a chromatin immunoprecipitation (ChIP) assay of porcine fibroblast cells was conducted to investigate whether the four MRFs act as direct trans-acting factors that bind to the XM_013981330.2:g.− 1805_−1810del site. The results of the ChIP assay demonstrated that MYF5 specifically bound to the q allele sequence, whereas the Q allele sequence that included the 6-bp deletion abolished its binding affinity. In the case of MYOD, MYOG, and MRF4, these MRFs were only able to interact partially with the Q sequence because the binding site was not completely abrogated by the 6-bp deletion (Fig 4A and Fig 5B). To critically evaluate the functional effect of the four MRFs on the transcriptional activities of the luciferase reporter constructs containing either the Q or q sequence of the MYH3 variant, we used transient cotransfection assays of porcine fibroblast cells. As shown in Fig 5C, when the q type of the MYH3 promoter construct was cotransfected with either one of the four MRF constructs or the four MRFs, a significant reduction in luciferase activity was observed compared to the activity associated with the Q type. Notably, coexpression of the q type of the MYH3 construct with all four MRFs led to significantly reduced luciferase activity compared with the activity in the fibroblast cells transfected with the empty control luciferase reporter alone. In contrast, overexpression of the four MRFs only weakly repressed the expression of

Allele frequency among pig populations indicates an Asian origin of the MYH3 KNP allele
To investigate the XM_013981330.2:g.−1805_−1810delfrequency across diverse pig breeds, we genotyped the MYH3 FSV in a wide panel of 377 pigs representing nine Asian domestic breeds, 12 European domestic breeds, and wild boars from Africa, Europe, and Asia (S5 Table). The Q allele occurred at high and intermediate frequencies in Chinese Neijang (0.80), Chinese Putian (0.80), Chinese Tongcheng (0.90), Chinese Xiang (0.54), and KNP (0.63) but was also found in Korean and East Russian wild boars albeit at low frequency. In contrast, the Q allele was almost absent in the European commercial breeds, as well as in both European and African wild boars. This allele distribution indicates that the MYH3 Q allele is of Asian origin and likely predates domestication. Berkshire and Middle White breeds are an exception compared with the other European breeds. The rare exception for these breeds is probably due to an introgression of the Asian pigs in Europe, which was known to have occurred since the 18th century [30,31]. Notably, we found that the Q allele is maintained at a moderate frequency despite its favorable effect on meat quality in KNPs. This could be due to the negative association of the Q allele with growth-related traits (Table 1). Sequence data indicated that nucleotide diversity in the critical region (12:55,073,130-55,561,243) was higher in Asian breeds (π = 0.0059) than in European breeds (π = 0.0038), which is consistent with the demographic history of pigs and in agreement with the results of previous studies (S6 Table) [32]. However, no decrease in the levels of genetic variability surrounding the MYH3 FSV was observed in the European breeds compared to the levels in the Asian breeds. Although no signature of selection was detected with Tajima's D statistics, an interesting possible exception was KNPs, for which Tajima's D = 2.50 at the promoter region may indicate balancing selection. Furthermore, we observed no reduction in diversity in the MYH3 and its promoter region because of domestication, neither in Europe nor in Asia. The absence of a significant selective footprint (as measured by Tajima's D or low diversity) in breeds with high frequency of the Q allele may be due to soft sweeps, which are much more difficult to detect than hard sweeps with these tests. In addition, Tajima's D is highly variable in the presence of ongoing selection [33].

Discussion
Although numerous genetic studies have mapped thousands of QTLs for complex quantitative traits [34], a very limited number of actual causative mutations have been detected in domesticated animals. This study is the first to show that MYH3 is a causative gene for myofiber type ratios and associated changes in IMF and adipogenesis in pigs and mice. MYH3 is known as an MYH isoform that is mainly expressed in various developmental stages including during the embryonic stage in skeletal muscle [35,36]. Additionally, coding mutations that occur in the MYH3 gene can cause muscle development disorders in humans [37,38]. Furthermore, we identified a structural variant in the promoter of the MYH3 gene that affects both muscle fiber-type specification and IMF accumulation using two independent crosses in pigs. We also discovered that the FSV alters the sequence of critical transcription factor binding sites located in the promoter of MYH3. Subsequently, we showed that the FSV of MYH3 can regulate transcription by differential binding of the four MRFs using a chromatin immunoprecipitation (ChIP) assay and transient transfection experiments in porcine fibroblast cells.
As shown in Table 1, the causative FSV was also related to growth traits in pigs. However, there was no significant body weight difference observed between transgenic and wild-type mice. A plausible interpretation of this result can be related to pleiotropy and linkage; if the FSV has a pleiotropic effect on both meat quality and growth traits, a body weight difference between transgenic and wild-type mice can be expected. Hence, the observed association does not necessarily indicate pleiotropy (i.e., the growth and meat quality related traits are influenced by the same causal variant), since the association could possibly be due to linkage (i.e., two different causal variants being in linkage disequilibrium, one influencing meat quality related traits and the other influencing growth related traits). Hence, it is possible that the variant affecting growth variation may not be included in the transgenic vector construction. This antagonistic effect of the FSV between the favorable meat quality traits and growth parameters can be concerned when this variant is considered in the implementation of current breeding programs. However, we are convinced that the MYH3 variant can be useful for inclusion in breeding programs; if a marker-assisted selection/gene editing strategy using this variant can be implemented together with traditional selection procedures, we can expect to obtain genetic gain in growth traits in a long-term perspective. In fact, we previously reported growth-related QTLs in the LK cross, but no major QTL was detected [39]. Given the polygenic architecture of growth, we argue that growth traits can also be improved by a combined approach using the MYH3 marker information and traditional selective breeding.
The moderate to high frequency of the Q allele among some of the Asian domestic pig breeds including KNPs along with the positive association of the q allele with growth performance indicate that some sort of balancing selection may have shaped the evolution of MYH3 in pigs. This case is similar to the case of the porcine RYR1 gene, which causes pale soft exudative meat and porcine stress syndromes in homozygotes but has a positive effects on muscle mass in heterozygotes [40]. An additional argument in favor of balancing selection could be the positive Tajima's D value in KNPs and the absence of a decrease in variability in European pigs (S6 Table); however, the exact mechanism remains to be elucidated since the Q allele is almost absent in European pigs (S5 Table).
In conclusion, we present the positional cloning of porcine MYH3 as a QTG and the identification of a 6-bp deletion FSV located in the 5 0 -flanking region that regulates transcription of the MYH3 gene and contributes to a major effect on both a � and IMF in pigs. This work enhances the understanding of the regulation of myotype ratios and associated changes in IMF in pig skeletal muscle. Furthermore, this information is immediately applicable for breeders who are actively involved in genetic improvement of pork quality through marker-assisted selection or introgression of the desired structural variant allele in breeding populations.

Ethics statement
All experimental procedures using pigs were conducted according to national and institutional guidelines and were approved by the Ethical Committee of the institution [Approval No.

Animals and phenotypes
Two independent cohorts were used in this study: a Landrace×KNP F 2 intercross and a Dur-oc×KNP F 2 intercross. The first cross was established as described previously [7]. Briefly, seventeen purebred Landrace pigs were mated with 19 purebred KNPs to produce a total of 91 F 1 progeny and 1,105 F 2 progeny (568 males and 537 females). For the second cross, nine purebred Duroc pigs were crossed with 5 purebred KNPs to produce 36 F 1 and 345 F 2 animals (187 males, 158 females). Animals in the two cohorts were raised at the experimental farm of the National Institute of Animal Science, Jeju, Republic of Korea. They were fed ad libitum, and males were not castrated. All F 2 experimental animals were slaughtered in the same commercial slaughterhouse. The means and standard errors of age at slaughter (days) were 199.2±0.35 (LK cross) and 195.7±0.66 (DK cross). The means and standard errors of carcass weight (kg) were 79.2±0.38 (LK cross) and 69.7±0.68 (DK cross). The approximate average slaughter ages of the pigs used for expression analysis were 170 (Landrace) and 180 (KNP) days. The data collection of meat quality-related traits (i.e., a � and IMF) was conducted as reported previously [8]. Traits related to muscle fiber characteristics were obtained using the myofibrillar ATPase staining method [41] and microscope counting.

Genotypes and genome-wide association study (GWAS)
All experimental samples were genotyped for 62,163 SNP markers using PorcineSNP60K BeadChip (Illumina). The SNP markers were filtered with a minor allele frequency <5%, a genotype call rate <90% and a P-value of the Chi-square test for Hardy-Weinberg equilibrium errors �0.000001. For the LK cross, a total of 40,628 SNPs on 18 autosomes remained for this GWAS. For the DK cross, a total of 39,964 SNPs on 18 autosomes remained for this GWAS. Error rates in Mendelian inheritance for all markers were also checked in the F 2 pedigrees using SNP marker information (<5%). A single-SNP marker analysis based on the genomewide rapid association using a mixed model and regression (GRAMMAR) approach was carried out to identify QTLs affecting meat quality-related traits in the two cohorts [42]. Each trait was adjusted for fixed (sex, batch and carcass weight) and random polygenic effects using the mixed-effects model method implemented in the ASReml program (VSN International). To estimate random polygenic effects, the kinship matrix computed from the F 2 pedigree was used. The residuals derived from the mixed-effects model were used as response variables in the linear regression analyses to correct for the familial relatedness within the F 2 intercross population. The GWA analyses were conducted under an additive model for each SNP using the PLINK program [43]. Bonferroni-adjusted significant (i.e., 0.05/40,628 markers, significant P-value = 1.23×10 −6 for the LK cross; 0.05/39,964 markers, significant P-value = 1.25×10 −6 for the DK cross) and suggestive (i.e., 1/40,628 markers, suggestive P-value = 2.46×10 −5 for the LK cross; 1/39,964 markers, suggestive P-value = 2.50×10 −5 for the DK cross) thresholds were established to address multiple testing issues in GWAS. The % phenotypic variance explained by the top-ranked SNP marker was (% Var SNP ) estimated using the following equation [44]: % Var QTL = [2p(1-p)a 2 / σ g 2 ] ×100, where p is the minor allele frequency of the SNP, a is the estimated allelic effect of the SNP, and σ g 2 is the additive genetic variance for each trait estimated by the ASReml program (VSN international). The % phenotypic variance explained by the QTL was (% Var QTL ) estimated by the following equation: [(RSS reduced −RSS full )/RSS reduced ] × 100, where RSS reduced and RSS full are residual sum of squares of statistical models of linkage analysis with and without the QTL term, respectively.

Joint linkage and linkage disequilibrium (LALD) analysis
High-resolution mapping of the QTL was conducted by jointly exploiting linkage and linkage disequilibrium using a haplotype-based approach. First, we used CRI-MAP ver 2.503, developed by Evans and Maddox (URL: www.animalgenome.org/bioinfo/tools/share/crimap), to construct the genetic linkage map of SSC12 using 935 SNP markers for the Landrace×KNP cross and 997 SNP markers for the Duroc×KNP cross. Second, founder haplotypes those found in the F 0 pigs were reconstructed using the DualPHASE program [13], which combines family (linkage and Mendelian segregation) and population (linkage disequilibrium) information in a Hidden Markov Model frame. A total of twenty founder haplotype clusters (K = 20) were used for the next step. Third, the haplotypes were incorporated into a mixed model including fixed (sex, batch and carcass weight), random (i.e., the twenty effects of founder haplotypes and animal effects), and random residual terms to perform fine mapping of QTL using the QxPAK ver 5.05 [45]. In addition, the LALD mapping with the effect of the most likely QTL position as cofactor was conducted to screen additional QTL signals in the chromosome of interest.

Marker-assisted segregation analysis (MASA) and in silico functional annotation in the 488.1-kb critical region on SSC12
MASA was performed in two ways: first, we conducted a half-sib QTL analysis to obtain evidence for QTL segregation within each F 1 sire-family. Identification of heterozygous Q/q F 1 sires was based on the results of the half-sib QTL analysis using their respective F 2 progeny (S7 Fig) [18]. Second, the QTL genotype of F 1 sires was determined by a log likelihood ratio test [19]. The F 1 sires were regarded as heterozygous if log 10 likelihood ratio score>2; homozygous if log 10 likelihood ratio score>−2; and undetermined genotype when −2 < log 10 likelihood ratio < 2 (S2 Table). The transcription start site of the MYH3 gene was identified using Promoter 2.0 [46]. To investigate whether the identified putative FSVs were located within transcriptional regulatory motifs, the MEME suite was used [20]. Subsequently, a detailed in silico annotation of the identified motifs was conducted by TRAP, JASPAR and PROMO programs, respectively [21][22][23]. These four programs were used with their default settings.

Genotype imputation and fine-mapping of the 488.1-kb critical region
Prior to the fine-mapping approaches, we imputed genotype data in the critical region using FImpute program which can utilize information from both pedigrees and populations [47]. The genotype imputation was conducted with the default settings of the FImpute program. For the imputation, we only used the variants from the 60K SNP chip and the 6-bp deletion variant for further fine mapping to fill in missing genotypes of the variants. To characterize the identified putative FSV in the 488.1-kb critical region, a Bayesian fine-mapping approach based on the CAusal Variants Identification in Associated Regions (CAVIAR) program [25]. The CAVIAR program incorporates association summary statistics (i.e., Z-scores) and LD correlation structures to compute the posterior probability of being candidate causal for each variant in the region of fine-mapping. The eCAVIAR program [26], a bivariate extension of CAVIAR, was also applied to fine-map the causal variant by colocalization analysis of association signals from a � and IMF. eCAVIAR calculates combined likelihood posterior probability (CLPP) to measure the degree of colocalization of the two QTLs by computing the probability that the variant is pleiotropic for both phenotypes. A threshold of 0.99 for both posterior probability and CLPP was applied to select candidate causal variants in the CAVIAR and eCAVIAR analyses. To evaluate the uncertainty of the fine-mapping analyses, 99% credible sets were constructed for both CAVIAR and eCAVIAR analyses (S4 Table).

Gene transcription analysis
Total RNA was isolated from cells and tissues using Trizol reagent (Ambion), according to the manufacturer's protocol. RNA was treated with DNase I and reverse transcribed into cDNA using the TOPscript cDNA Synthesis Kit (Enzynomics). For cDNA synthesis, 5 μg of each sample RNA was incubated at 55˚C for 60 min and at 95˚C for 5 min. Each cDNA was used as a template for qRT-PCR amplification in combination with specific primers (S7 and S8 Tables). We performed qRT-PCR using the QuantiTect SYBR Green PCR Kit (Qiagen) and the Rotor-Gene Q thermal cycler (Qiagen). The qRT-PCR experiments were conducted based on the MIQE guidelines [48]. qRT-PCR was performed for 40 cycles at 95˚C for 20 sec, at 60˚C for 20 sec, and at 72˚C for 20 sec. Transcription levels were normalized to those of GAPDH mRNA. GAPDH has been used as the reference gene in several expression studies related to myogenesis and adipogenesis [49][50][51][52]. Data were analyzed using the ΔΔC t method [53].

NGS and Sanger sequencing
A massively parallel sequencing technology was used to identify SNP markers in the porcine whole genome, including the MYH3 locus on SSC12, using genomic DNA of the 24 F 1 sires and the parental pigs [Landrace (n = 17) and KNPs (n = 19) for the LK cross; Duroc (n = 9) and KNPs (n = 5) for the DK cross] from the two crosses (i.e., 18 pigs from the LK cross; 6 pigs from the DK cross). Adapter-ligated DNA libraries were prepared for single and paired-end sequencing. All sequence data were produced using HiSeq X (Illumina) according to the standard protocol. The average coverage depth was approximately 30×. In addition, the Sanger sequencing method using BigDye Terminator 134 v3.1 Cycle Sequencing Kit (Applied Biosystems) was applied to determine the sequence of the 5' and 3' flanking regions and exon regions of the porcine MYH3 gene using DNA samples from the parental animals of the two cohorts [i.e., Landrace (n = 17) and KNPs (n = 19) for the LK cross; Duroc (n = 9) and KNPs (n = 5) for the DK cross].

Generation of transgenic (TG) mice
All mice (C57BL/6 background, male, 4 months of age) used in the study were maintained in the Korea Research Institute of Bioscience and Biology (KRIBB) animal facility under pathogen-free conditions in a temperature-controlled climate at 22±2˚C and with a 12 h light/dark cycle. All animals had free access to standard chow and water during the experiments. For the convenience of cloning, the CDS of the porcine MYH3 gene was divided into four parts for in vitro synthesis (Bioneer). We lined them up end-to-end to make a 5,859 bp full-length open reading frame (ORF) and inserted it into the position between the XbaI and EcoRI of a pCAGGS-EGFP-Puro vector (S6A Fig). In addition, a Flag-sequence was appended to the 3 0 end of the ORF to allow for the detection of the expression of recombinant protein directly through Western blotting. TG-mice were generated via DNA microinjection. PCR analysis was applied to test the construction of TG-mice using a primer set (forward: 5 0 -CCG AGA GCT GGA GTT TGA-3 0 ; reverse: 5 0 -CTC CCA TAT GTC CTT CCG AGT-3 0 ). Quadriceps muscles were excised from 4-month old WT-and TG-mice and used for further experiments.

Western blotting analysis
Tissue protein lysates were prepared with RIPA buffer containing a cocktail of protease inhibitors and then quantified using a Protein Assay Kit (Bio-Rad). Protein samples were separated by SDS-PAGE and transferred to a PVDF membrane. After blocking in TBST solution containing 5% skim milk, membranes were incubated overnight at 4˚C with specific antibodies (S9 Table). Expression signals of each protein were detected using ECL reagents (GE Health Care) with secondary antibodies. Luminescent densities were measured using a LAS-3000 Luminescent Image Analyzer System (Fujifilm).

Histological analysis of mice
Traits related to muscle fiber characteristics were obtained using the myofibrillar ATPase staining method described by Brooke and Kaiser (1970) and microscope counting. For IHC analysis, paraffin sections (4 μm) were fixed in 4% PFA, permeabilized with 0.1% Triton X-100 and left for 1 h in serum containing blocking solution. Anti-MYH7, anti-MYH4 and anti-flag M2 antibodies were diluted to 1:100 in PBS and incubated overnight at 4˚C. After washing with PBS containing Tween 20, the sections were incubated for 1 h with fluorescent conjugated secondary antibodies and visualized under a fluorescence microscope. DAPI was used for nuclei counterstaining.

Measurement of triacylglycerol (TAG), triglycerides and free fatty acids (FFAs)
Both WT and TG mice were fasted for 6 h before sacrifice, and dissected quadriceps muscle and blood were obtained. TAG, triglycerides and FFAs were assayed using a Triglyceride Assay Kit (ABCAM) and a Free Fatty Acid Assay Kit (ABCAM). The levels of TAG, triglycerides, and FFAs were measured by the fluorescent intensities at an absorbance ratio of 535/590 nm wavelength. Finally, the levels of TAG, triglycerides, and FFAs were calculated based on typical standard curves.

Luciferase and chromatin immunoprecipitation (ChIP) assays
Primarily cultured porcine fibroblast cells, originating from biopsied porcine ear tissues, were cultured in DMEM (Life Technologies) containing 10% FBS (HyClone) and 1×penicillinstreptomycin reagent (Gibco). Cells were grown at 37˚C in humidified air containing 5% CO 2 and the medium was changed every 2 days. The porcine fibroblast cells have been used for our other studies [28,29]. All MYH3 luciferase reporter constructs were generated by subcloning the porcine MYH3 promoter in front of the luciferase gene in the pGL3 basic vector. The MYH3 luciferase reporter constructs and internal control (pRL-SV40) vectors were cotransfected into the cells using Lipofectamine 2000 (Invitrogen). Luciferase assays were performed using a Dual-Luciferase Reporter Assay System (Promega). Transfected cells were rinsed in PBS and then lysed in 1× passive lysis buffer, after measuring firefly (reporter construct) and Renilla (internal control) luciferase values using the VICTOR Multilabel Plate Reader (Perkin Elmer). MRF genes (i.e., MYF5, MYOD, MYOG, and MRF4) were cotransfected with an MYH3 promoter and an internal control vector into porcine fibroblast cells. After 48 h, MYH3 promoter activity was measured using the Dual-Luciferase Reporter Assay System. The influence of MYH3 promoter activity was determined according to MRFs binding in the functional sequence variant position. ChIP assays were carried out using a Chromatin Immunoprecipitation Assay Kit (Millipore). Precleared chromatin was immunoprecipitated with 4 μg of the HA antibody (Sigma). The obtained DNA samples were verified by PCR analysis (forward 5 0 -GGT CCT ACT GGC GCT TAA GAC AGA-3 0 ; reverse 5 0 -GGT TGT GGC AGG AAT GTG TGA TTG-3 0 ) for the MRFs binding motif on the MYH3 promoter region.

Statistical analysis
The results are expressed as the mean±standard error. Minitab version 17 (Minitab Inc.) and MS Excel (Microsoft Inc.) were used to evaluate statistical significance using two-sided Student's t-test or two-sided Welch's t-test when comparing two groups or analysis of variance for multiple group comparisons. The F-test implemented in the Minitab program was applied to assess variance equality between two groups (P-value 0.01). P<0.05 was considered as significant for the two-sample t-tests. Unless otherwise stated P-values are nominal; the nominal Pvalue represents the probability of false positives for a single test.

Population genetic analysis
The XM_013981330.2:g.−1805_−1810del was genotyped in 377 wild and domestic pigs distributed worldwide (S5 Table). Furthermore, to characterize the genetic variability surrounding the region, a total of 160 NGS data samples were analyzed (S6 Table); 65 sequences were downloaded from SRA [54][55][56][57][58]; and 10 KNP, 10 Duroc, and 10 Landrace NGS data were obtained in this study. The sequences included wild boars (10 Korean and 10 of European origin) and domestic pigs (34 Chinese and Korean and 41 European). The Chinese pig breeds were Meishan and Toncheng, and the European pig breeds were Berkshire, Duroc, Iberian, Landrace, and Large White. Downloaded samples from SRA had been sequenced to an average depth of approximately 11×, whereas the depth of those in this study (i.e., KNPs, Duroc and Landrace) was approximately 30×. Alignment was carried out with BWA 0.7.15-mem option [59]. The bam files were then realigned around indels with the GATK IndelRealigner tool [60], and PCR duplicates were removed with the picard MarkDuplicates option (http:// broadinstitute.github.io/picard/). SNP calling was performed with SAMTOOLS/BCFTOOLS suite version 1.3.1 for each individual separately [61]. SNPs were called in positions with depth bounds between 5× and twice the average depth rounded to the nearest upward integer; further, a minimum mapping (RMS) quality of 20 and a base quality of 20 were required. Finally, SNPs with a minimum quality of 10 were retained. To estimate nucleotide diversity, in addition to heterozygous positions, the number of bases sequenced was also required. We extracted these regions with minimum and maximum depth using SAMTOOLS depth, further filtering by minimum map and base qualities and then, using BEDTOOLS [62], we intersected these regions with the homozygous blocks provided in the individual gvcf file. This resulted in a modified gvcf file in which both SNPs and homozygous blocks had been filtered by the same criteria. Diversity and Tajima's D estimates were as in accounting for missing data [63], and as implemented in MSTATPOP software [64]. on SSC12 for a � and IMF traits in the LK cross (B) Linkage mapping results on SSC12 for a � and IMF traits in the DK cross. The y-axis represents the F-value test statistic. The marker map with genetic distance between DNA markers in Kosambi cM is given on the x-axis. The thick horizontal line indicates the 1% chromosome-wide significant threshold, and the thin horizontal line indicates the 5% chromosome-wide significant threshold. The QTLs were colocalized in the region encompassing rs81437379. Linkage analysis for mapping QTL was performed using the GridQTL program (URL:www.gridqtl.org.uk).