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Immunosuppressive effect of Pseudomonas aeruginosa endotoxin on lymphocyte transformation in vitro.

In order to prove the immunosuppressive activity of an endotoxic fraction from pathogenic Ps. aeruginosa, the lymphoblastic transformation test was used. The results have shown that lymphocytes cultured in presence of PHA and endotoxin have a decreased rate of DNA synthesis and oxygen uptake. On the contrary, there seemed to be a slight relative increase of RNA and protein synthesis as compared with PHA-transformed cells.