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Comparison of a gelation and a chromogenic Limulus (LAL) assay for the detection of Gram-negative bacteria, and the application of the latter assay to milk

Summary When a chromogenic Limulus Amoebocyte Lysate (LAL) assay and a tube gelation LAL assay were compared for the detection of Gram-negative bacteria, using a strain of Pseudomonas putida, the detection level (∼ 103 cfu/ml) and cost of the assays were approximately the same for both assays but the reading was more precise for the chromogenic substrate assay. A modified chromogenic assay was devised for detection of Ps. putida in milk.