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Prediction of the secondary structure and the B cell epitope of TRAM protein and determination of its immunogenicity

Objective To predict the secondary structure and the B cell epitopes of TRAM protein, and to provide research basis for developing monoclonal antibody against TRAM. Methods Based on amino acid sequence of TRAM protein, the analysis of the flexible regions of secondary structure and the B cell epitopes of TRAM protein were predicted by software of nnpredict and Goldkeu. The peptide of the epitopes was synthesized and used for immunizing rabbits. The immunogenicity of the peptide was determined. Results The computer predicted that most possible epitopes of TRAM protein were within or near its N-teminal No. 216-229, which accorded with its hydrophilicity, accessibility, flexibi- lity, and which located in extend and/or ruleless convolute frame of the protein for the secondary structure. Synthesized peptides induced high titer of antibody and the polyclonal antibody to TRAM has high specificity. Conclusion N-teminal No. 216-229 of TRAM protein may be a B cell epitope, which provides a theory basis for the production of site-directed monoclonal antibody.