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Bicarbonate abolishes intracellular alkalinization in mitogen‐stimulated 3T3 cells

An increase in intracellular pH (pHi) following mitogenic stimulation has been reported in a variety of mammalian cells (W. Moolenaar, Annu. Rev. Physiol., 48:363–376, 1986; E. Rozengurt, Science, 234:161–166, 1986). This increase is currently believed to constitute a “permissive” signal in the process of cell activation (A.E. Lagarde and J.M. Pouyssegur, Cancer Biochem. Biophys. 9: 1–14, 1986). Since the majority of studies of this phenomenon have been conducted in the nonphysiological milieu of bicarbonate‐free solutions, we have undertaken a study of the effects of bicarbonate and CO2 on mitogen‐induced intracellular alkalinization in NIH 3T3 cells.