{'fraction_non_alphanumeric': 0.025329280648429583, 'fraction_numerical': 0.005065856129685917, 'mean_word_length': 5.024390243902439, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Influence of Selected Natural Products on Neutral Endopeptidase Activity and β-Amyloid Production in SK-N-SH Cells

Abstract Neutral endopeptidase (NEP) and angiotensin-converting enzyme (ACE) belong to the major Aβ-degrading enzymes. We currently demonstrate that apigenin, luteolin, and curcumin are able to enhance the activity of NEP and ACE. In this research, we examined the influence of these substances on the amount of endogenously produced and secreted Aβ of neuroblastoma cells. We found that treatment of the cells for 72 h with apigenin, luteolin, and curcumin decreases the endogenous Aβ 1-42 level of SK-N-SH cells significantly, which indicates that the upregulation of both NEP and ACE activity leads to a decrease of Aβ in the medium. Furthermore, we showed that all of these substances are able to increase the intracellular cAMP level, which verifies our previous supposition that the enhancement of cellular NEP activity is correlated with an increase of cAMP level.