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Rare RNF213 variants and the risk of intracranial artery stenosis/occlusion disease in Chinese population: a case-control study

Background RNF213 rare variant-p.R4810K (rs112735431) was significantly associated with intracranial artery stenosis/occlusion disease (ICASO) in Japan and Korea and to a lesser degree in China. Considering the allelic heterogeneity, we performed target exome sequencing of RNF213 with the aim to identify the rare variants spectrum and their association with ICASO in a Chinese population and further to explore whether the rare variants carrier patients present specific clinical phenotype. Methods Target exome sequencing of RNF213 was performed in 250 ICASO patients using FastTarget sequencing technology. Various filtering process were used to select the candidate variants. Control individuals were obtain from 1000 Genome Project (208 Chinese samples) and GeneSky in-house database (1007 samples). Gene-based association analyses were conducted to identify the association between RNF213 rare variants and ICASO. The clinical characteristics of rare variant carriers and non-carriers were compared using Chi-squared test or Fisher’s exact test. Results After filtration, 18 rare variants were identified in 39 patients. Gene-based association test showed that rare variants of RNF213 were significantly associated with ICASO (Minor allele frequency < 0.05, WSS p = 4.88 × 10− 10; SKAT p = 9.68 × 10− 6; SKAT-O p = 3.42 × 10− 9). There were no significant clinical characteristic differences other than the diagnosis age which was older in the carriers than the non-carriers (60.5 ± 6.2 vs 57.3 ± 8.9 years old, p = 0.028). Conclusion Rare variants of RNF213 are associated with ICASO in Chinese. However, there are limited genetic diagnosis values of the gene due to no specific phenotypic presentation in the carriers and non carrier patients. Electronic supplementary material The online version of this article (10.1186/s12881-019-0788-9) contains supplementary material, which is available to authorized users.


Background
Intracranial artery stenosis/occlusion disease (ICASO) is one of the main causes of ischemic stroke [1]. It is more prevalent in Asians than in Westerners. In Asia, 30-50% of strokes and more than 50% of transient ischemic attacks are caused by ICASO [2]. Atherosclerosis, chronic injury, infection, cardioembolism, arterial dissection, vasculitis, moyamoya disease (MMD) and rare autoimmune diseases are common cause of ICASO [3]. The most common cause of ICASO is atherosclerosis, which is commonly caused by acquired factors, such as smoking, hypertension, diabetes mellitus and dyslipidemia [3]. However, conventional risk factors only explain part of risks and the underlying etiologies is largely unknown. Many studies supported a genetic risk role of ICASO [3][4][5][6][7][8][9][10].
The ring finger protein 213 (RNF213) was found to be a susceptibility gene for MMD in East Asian population and the founder rare variant-p.R4810K (rs112735431) was subsequently identified to be associated with non-MMD ICASO which showed stenosis or occlusion of the intracranial major arteries but do not meet the diagnostic criteria of MMD [3][4][5][6][11][12][13][14][15][16][17][18][19][20][21]. Meta-analysis showed that RNF213 p.R4810K increased the risk of ICASO in Chinese (odds ratio, 5.59; 95% confidence interval, 2.12-14.75; p = 0.001), but the effect size was significantly lower than that in Japanese and Korean (odds ratios, 10.71 and 28.52; 95% confidence interval, 3.97-28.91; and 11.04-73.67, respectively) [22]. Further studies found that many non-p.R4810K RNF213 rare variants have been identified in MMD patients in ethnically diverse populations, including Asians, whites, and Hispanics, while p.R4810K is absent in non-Asian populations, which illustrating allelic genetic heterogeneity of RNF213 in different ethnic population [23]. Whether other RNF213 rare variants contribute to Chinese ICASO is still unknown. Considering the allelic heterogeneity, we performed target exome sequencing of RNF213 with the aim to identify the rare variants spectrum and their association with ICASO in a Chinese population and further to explore whether the rare variants carriers present specific clinical phenotype or not.

Study population
Seven hundred and fifteen ICASO patients were recruited from two university affiliated hospitals (615 from the Second Hospital of Hebei Medical University and 100 from XiangYa Hospital of Central South University) previously [9]. Among them, only 6 patients carried RNF213 p.P4810K variants. In order to investigate whether other RNF213 rare variants associated with ICASO or not, 250 patients were randomly selected from our previous individuals. Allelic frequencies of control individuals were obtained from 1000 Genome Project Chinese Han Population (208 samples, http://www. internationalgenome.org/home) and GeneSky in-house database (1007 samples, http://www.geneskybiotech. com/index.html). This study was approved by the Medical Ethics Committee of Central South University and all patients signed an informed consent before participating.

Diagnosis of ICASO
The ICASO was diagnosed by digital subtraction angiography, computed tomography angiography or magnetic resonance angiography and defined as stenosis or occlusion at terminal and/or proximal portions of the intracranial major arteries without abnormal vascular networks in the basal ganglia that did not meet the diagnostic criteria of MMD. At least 2 physicians(1 radiologist and 1 neurological physician) were required to interpret angiography images results. Demographic information including age, gender, behavioral risk factors (tobacco and alcohol consumption), disease histories of hypertension, diabetes, hyperlipemia were collected by interview or questionnaire. Patients with potential diseases including cardioaortic embolism, coagulopathy, vasculitis, arterial dissection that may leads to cerebral apoplexy were excluded.

FastTarget sequencing
Genomic DNA with high quality suitable for sequencing was extracted from the peripheral blood leukocytes using TIANamp Blood DNA Extraction Kit (TIANGEN BIO-TECH CO., LTD, Beijing, China). Protein coding sequences of RNF213 were selected and a custom panel covering the target regions was designed. Capture target regions contained flanking sequences of 25 bases at the borders of each exon. Target

Variant filtration
After sequencing, a series of filters were used to prioritize variants. Variants were considered higher priority if they were: (a) predicted to affect protein-coding sequences (including nonsynonymous, frameshift deletion, stop-gain variants in exonic or splicing regions); (b) less common in reference databases (Minor allele frequency was less than 0.05 in 1000 Genome project Chinese Han population [ftp://ftp.1000genomes.ebi.ac. uk/vol1/ftp/release/20130502/] and GeneSky in-house Database [http://www.geneskybiotech.com/]); (c) predicted as damaging, possibly damaging or unknown by SIFT or PolyPhen-2; (d) sequencing read depth was higher than 10x.

Statistical analysis
Descriptive analyses were conducted using SPSS 21.0 software (SPSS Inc., Chicago, IL, USA). Continuous variable (age) was presented as the mean ± standard deviation (SD). Categorical variables (hypertension, diabetes, hyperlipemia, smoking, drinking, clinical symptoms and characteristics of stenosis) were presented as proportions. The Weight Sum Statistic (WSS), the Sequence Kernel Association Test (SKAT) and the SKAT-optimal test (SKAT-O) were used to evaluated the statistical significance of variants between affected and control individuals. A continuous weight function proposed by Madsen and Browning was performed to focus on the lower frequency and rarer variants, this weight score method allows both low frequency (MAF < 0.05) and rare (MAF < 0.01) variants to contribute to the overall statistic by calculating the logistic weights for each variant and applying them to the analysis according to the following formula [24]: Student's t-test, Chi-squared test or Fisher's exact test were used to compare the characteristic differences between carriers and non-carriers of RNF213 rare variants. Threshold of statistical significance was set at p value less than 0.05.

Characteristics of participants
Of all the 250 patients with ICASO, 77(30.8%) were female and the average age was 57.8 ± 8.6 years (range: 28-70 years). The information of the vascular risk factors, clinical symptoms and characteristics of cerebral artery stenosis were summarized in Table 1.

Identification of low frequency and rare variants in target region
The average value of mean target coverage was 527x, 88.87% of the reads had a Phred-like quality score (Q-score) greater than 30.The proportion of the samples obtained mean coverage >140x was 94.24%. After variants filtration, 18 variants were found in 39 patients ( Table 2). The detail information of these rare variants was shown in Table 3.

Clinical characteristics of patients with and without RNF213 rare variants
Among the 250 ICASO patients, we found 39 patients carried RNF213 rare variants and compared the clinical characteristics between the carriers and non-carriers. The results showed that patients with RNF213 rare variants were older at diagnosis (60.5 ± 6.2 vs 57.3 ± 8.9 years old, p = 0.028). Risk factors of ICASO (including smoking, alcohol drinking, hypertension, diabetes, hyperlipemia), clinical symptoms and characteristics of cerebral artery lesions (quantity of lesions, presence of anterior and posterior ICASO) showed no significant difference between two groups (Table 5).

Discussion
In this study, we sequenced the exome of RNF213 in 250 ICASO patients and identified 18 rare variants in 39 patients. Gene-based association test showed that rare variants of RNF213 were significantly associated with ICASO. However, there were no significant clinical characteristic differences between the rare variants carriers and non-carriers. RNF213 founder mutation-p.R4810K (rs112735431) was identified to be significantly associated with MMD and non-MMD ICASO in East Asia. Other than p.R4810K, numerous rare variants also were identified in MMD cases in previous studies. In Japan, Kamada et al. identified 3 non-p.R4810K rare variants of RNF213 (p.M3891 V, p.V4567 M and p.V4765 M) among MMD patients [11]. Subsequently, Moteki et al. found 16 rare coding variants of RNF213 in MMD cases by exome sequencing, including 2 previously identified variants (p.V4567 M and p.V4765 M) and 14 novel variants (Additional file 1: Figure S1, Additional file 2: Table S1) [25]. In China, researchers identified 40 RNF213 rare variants among MMD patients. All of these variants   were specific in Chinese MMD except p.D4013N (found in Caucasian) and p.R4062Q (found in Caucasian and Japanese) [12,14,18,21,26]. Moreover, p.D4863N, p.E4950D and p.A5021V were particularly identified among Chinese MMD patients in several studies [12,14,21]. In non-East Asian countries, numerous RNF213 variants were identified. Cecci et al. identified 10 variants only in Caucasian MMD patients [27] and Shoemaker et al. identified 22 variants in multiple non-East Asian population [26] (Listed in Additional file 2: Table S1). Whether these rare variants or other RNF213 rare variants associated with ICASO like p.R4810K or not is unclear currently. In this study, we performed target exome sequencing of RNF213 in ICASO patients and found 18 rare variants in 39 patients. All the 18 rare variants identified were novel and not have been found in previous MMD studies. This result suggested that RNF213 rare variants spectrum of Chinese ICASO is distinct from MMD, locus heterogeneity of RNF213 is highly indicated between ICASO and MMD. Recent studies suggested that RNF213 genotypes were associated with MMD phenotypes, numerous studies confirmed that the homozygous p.R4810K variant carriers present earlier onset age, severe symptoms and prognosis [13,17,28]. Zhang et al. demonstrated Chinese MMD patients with RNF213 p.R4810K were younger at diagnosis than those without RNF213 rare variants (25 vs 29 years old, p = 0.049), more ischemic cases and more likely to occur at posterior cerebral artery [21]. In addition, researchers also identified the association of RNF213 variants and ICASO phenotypes. Bang et al. indicated that Korean ICASO patients with RNF213 p.R4810K variant were younger at diagnosis than those without the variant (52.6 ± 9.6 vs 56.8 ± 12.7 years old, p = 0.027) [6]. But our study showed that mean age at diagnosis of RNF213 rare variants carriers was older than that of non-carriers. Considering the complicated current medical status of China, it is difficult to confirm the precise onset age during the slow progression of ICASO unless the patients seek a medical treatment at the early stage of ICASO (such as recurrent headache, dizziness and transient ischemic attack). The majority of patients (66.6%) in our study suffered from severe acute cerebral infarction as initial clinical symptoms, so the age at diagnosis in our research may not be able to reflect the true onset age of ICASO.
In our study, the presence of anterior and posterior ICASO showed no significant difference between RNF213 rare variants carriers and non-carriers. This result is consistent with Bang's study. They reported that the presence of proximal carotid artery and posterior ICASO showed no significant difference between RNF213 p.R4810K carriers and non-carriers [6]. But in Shinya's study, all of 10 RNF213 p.R4810K carriers suffered anterior ICASO and no one suffered posterior ICASO [10], they defined the posterior circulation as the basilar artery and vertebral artery, patients with posterior cerebral artery stenosis were not found in their case series. The difference of patient selection may explain the discrepancy. ICASO is caused by environmental and genetic risk factors. Though some specific rare variants of RNF213 were identified in Chinese ICASO patients, but there were no specific clinical characteristics of the RNF213 rare variants carriers, which illustrating that rather than RNF213 variants there may be other risk factors of ICASO in Chinese. Studies focus on multiple factors including genetic and environmental are needed.
The limitation of this study should be mentioned. First, the sample size of our study was relatively small and the strictly matched cerebrovascular disease-free controls were not available; the reference allele frequencies of rare variants in unaffected individuals were obtained from 1000 Genomes Project and Genesky in-house database. Second, atherosclerosis is the most common cause of ICASO, it usually caused by acquired factors, such as hypertension, diabetes mellitus, dyslipidemia, and smoking. However, these information were not available in the control databases, so we cannot take these environmental risk factors as covariate when we implemented the gene-based association test.

Conclusion
Rare variants of RNF213 are associated with ICASO in Chinese, but there were no specific clinical characteristics of the RNF213 rare variants carriers. Genetic diagnosis values for this gene among Chinese ICASO patients are limited. Rather than RNF213 variants there should be other risk factors of ICASO in Chinese. Studies focus on multiple factors including genetic and environmental are needed.

Availability of data and materials
The data that support the findings of this study are available from Genesky Bio-Tech Company but restrictions apply to the availability of these data, which were used under license for the current study, and so are not publicly available. Data are however available from the authors upon reasonable request and with permission of Genesky Bio-Tech Company.
Authors' contributions TZ, BL participated in the patient recruitment, sample collection and clinical data explanation. XL, SH, JL, JD and JY carried out the experiments and data analysis. XL, HT and JY took charge of study design, coordination and drafted the manuscript. All authors read and approved the final manuscript.