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Centrifugal and anticoagulant induced variations in platelet rich plasma and their influence on platelet aggregation.

Variations in the centrifugal force (g) used to prepare platelet-rich-plasma (PRP) alter not only the number of platelets therein but also their size and reactivity. Anticoagulants also affect these parameters. According we suggest that PRP preparations need to be strictly standardised. Critical comment on the use of the platelet aggregation model as a means of assessing 5-hydroxytryptamine receptor function is included.