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[Cloning and sequence analysis of the ribosomal DNA ITS gene of Leishmania donovani isolates from hill foci of China].

OBJECTIVE
To determine the nucleotide sequence of the ITS (internal transcribed spacer) gene of Leishmania donovani isolates from hill foci (L. d. SC10 and L. d. 6), and to find out the difference of the gene sequences between the two isolates.


METHODS
Specific ITS fragments from nuclear DNA of two Leishmania isolates were amplified by PCR, cloned into pMD18-T vector, and finally sequenced by the dideoxy chain termination method.


RESULTS
Sequence analysis showed that the amplified DNA fragments of the two isolates were 1027 bp ( L. d. SC10) and 1 028 bp (L. d. 6) respectively, showing a sequence difference.


CONCLUSION
Sequence difference exists between the Leishmania isolates L. d. SC10 and L. d. 6 from hill foci in China.