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Convection compensated electrophoretic NMR (CC-ENMR) for structure characterization of mixed proteins in biological buffer solutions

A novel ENMR method-Convection Compensation ENMR (CC-ENMR)-has been developed, which permits selective detection of electrophoretic motion of ionic species. The convection generated ENMR spectral artifacts were removed by gradient moment nulling technique, whereas polarity switching of the applied DC electric field canceled out the flow compensation effect for ionic movement driven by the electric field.