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Effect of growth regulators on Callus production in two medicinally important plants of cucurbitaceae

An in vitro protocol was developed for callus production from two medicinally important plants of family Cucurbitaceae viz. Luffa Cyindrica Linn. and Citrullus colocynthis Linn. For callus formation, growth regulators such as auxin and cytokinins are required. Callus can be maintained on MS medium indefinitely by frequent subculturing in fresh medium by transferring a small pieces of the same to fresh agar medium. Different explants used in the present investigation responded differently to various concentration and combinations of hormones. Callus initiation took place after 12-18 days of inoculation of explants in MS medium supplemented with growth regulators in both the plants. The callus cultures thus obtained were maintained on MS medium for 12 months by subculturing the tissue in to fresh MS-medium with same growth hormone concentration at 3-4 weeks intervals. At different time intervals of subculturing growth indices of both plants calli were calculated. Three replicates of each of the tissue samples were examined and their mean values were calculated.