{'fraction_non_alphanumeric': 0.026622296173044926, 'fraction_numerical': 0.012479201331114808, 'mean_word_length': 5.233160621761658, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Experimental study of fast diagnosis of mycotic keratitis using molecular biology technical].

OBJECTIVE
To establish a method for fast diagnosis of mycotic keratitis using multi-PCR system.


METHODS
Detecting 9 important medical fungi species (Fusarium solani, Fusarium moniliform, Fusarium equiseti, Fusarium graminum, Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Penicillium implicatum and Curvularia lunata) through a multi-PCR system. This method was used to identify the clinical cultured isolates and clinical samples.


RESULTS
In this multi-PCR system, Fusarium species amplified a fragment of 360 bp, Aspergillus species amplified a fragment of 430 bp, Penicillium implicatum species amplified a fragment of 245 bp, and Curvularia lunata species amplified a fragment of 300 bp. Human DNA and DNA of other microorganism in human ocular infection obtained negative results in this multi-PCR system. The sensitivity of the multi-PCR system was 10 fg.


CONCLUSIONS
Important clinical fungi could be detected and identified by the multi-PCR system. The multi-PCR method was proved to be a fast, sensitive and specific technology and has a good prospect in clinical application.