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Immunological findings in sensory carcinomatous neuropathy. Application of peroxidase labelled antibody.

An antibody against neurones, which has previously been demonstrated by immunofluorescence, is present in the sera of patients with sensory carcinomatous neuropathy. This antibody was demonstrated by a new technique, namely the use of purified peroxidase-labelled sheep anti-human γ-globulin. In cryostat sections of brain treated with a first layer of serum antibody followed by a second layer of peroxidase labelled anti-human γ-globulin, heavy coarse brown deposits in nerve cells only from different parts of the central nervous system indicated a positive reaction. The special technical precautions necessary to obtain specific staining of neurones are outlined.