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Comparison of the visible spectrum of reflected light from muscle tissue in rats breathing varying amounts of oxygen in nitrogen or perfused with saline.

One of the most important problems in the treatment of injuries caused by high velocity missiles is to find the borderline between viable and non-viable muscle tissue. Technical aids using spectrophotographic principles are designed. The present investigation was performed in order to study the absorption of incident light in muscle tissue spectrographically and to compare vital muscle tissue supplied with blood of varying degrees of oxygen saturation in rats. Transmission of light through transparencies was analysed spectrographically. Transmission of muscle tissue without blood is responsible for approximately 60% of the total transmission. Rats breathing 100% oxygen differ in transmission significantly from the control group (breathing air) within the wavelength region of 430--570 nm. Rats breathing 10% oxygen show no significant difference to the control group.