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Pharmacological evidence for a spasmogenic agent in Datura root extract tested on dispersed smooth muscle cells of guinea‐pig ileum

The effect of acetylcholine, scopolamine, and Datura leaf and root extracts on the contractility of dispersed muscle cells of guinea‐pig ileum was studied. Scopolamine and crude Datura leaf extract alone or together had no significant effect on contraction of smooth muscle cells. Acetylcholine and Datura root extract either alone or together caused a significant (30%) contraction. Datura root extract reversed the effect of both scopolamine and Datura leaf extract suggesting the presence of an unknown spasmogenic factor in Datura root extract.