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Nucleotide sequences of ten mitochondrial tRNA genes in yeast Hansenula wingei.

Yeast Hansenula wingei (strain 21) has a circular mitochondrial genome. Recently, we have constructed a physical map of the mtDNA of which size is 27 kb (1). The location of large and small rRNA genes has been assigned to the map. To know the gene organization, the HindIII fragments (H3, H4, H5, H6 and H7 of Figure 1) containing large and small rRNA genes were cloned and sequenced. During DNA sequencing, we found ten mitochondrial tRNA genes by their cloverleaf structures of putative transcripts and designated them for accepting amino acids by their anticodon sequences.