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A Quartz-crystal microbalance study of adsorption behaviors of artificial glycoconjugate polymers with different saccharide chain lengths and with different backbone structures

Adsorption of glycopolymers is essential for the development of biologically active surfaces employing oligosaccharides as recognition signals. The adsorption behavior has been investigated by quartz-crystal microbalances (QCMs) with use of two different series of glycopolymers: (1) polystyrenes carrying maltooligosaccharides with different saccharide lengths and (2) lactose-carrying polymers with polystyrene and polyphenylacrylamide main chain structures. These glycopolymers were adsorbed on the hydrophobic surfaces of self-assembled monolayers (SAM) of 1-octadecanethiol and cast films of polystyrene from their aqueous solutions, but not on hydrophilic surfaces of SAM of cystamine hydrochloride. The apparent association constants (K a = 10 4 ∼ 10 6 M -1 in molarity of the monomeric unit) and maximum adsorption amounts (Δm max ) on 1-octadecanethiol SAMs and polystyrene cast films were estimated from the Langmuir adsorption isotherms. These values depended on the side-chain oligosaceharide lengths and the main chain chemical structures of these glycopolymers. The adsorption behavior has been discussed in correlation with the desorption of the adsorbed polymer in an aqueous surfactant solution, the partition of the glycopolymer in a water/1-octanol system, and the adsorption amont of bovine serum albumin (BSA) onto the glycopolymer-adsorbed surface. We propose that these polymers are adsorbed in loop-train-tail conformations in which the ratio of the loop-tail portion to the train portion depends on the hydrophilic-hydrophobic property of the polymers.