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Developing the Vertical Comet Assay and Image Handling Software for the Radiological Research Accelerator Facility Microbeam II

The vertical comet assay is a relatively new technique for quantitatively measuring radiation induced DNA damage in cells. The vertical comet assay provides potential for measuring DNA strand breaks in a dense monolayer of plated cells. This paper explores the successes and challenges of developing a vertical comet assay system for use with the current Microbeam II alpha particle source at RARAF. Additionally, the program BioComet, which was developed for confocal comet imaging, is explored in depth.