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Herpes Simplex Virus Type 2 Displays Atypical Melting-Temperature Profiles at Low Viral Titers

ABSTRACT Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (Tms) of the hybridization probes. The efficacy of Tm profiling with low DNA concentrations was evaluated in this study.