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Three Approaches to the Radioimmunoassay of Human β‐Thromboglobulin

Summary. Three radioimmunoassays for the measurement of β‐thromboglobulin are described. The standard method, using antiserum in solution, could be used to measure plasma concentrations of β‐thromboglobulin with the results available after 2–3 d. The use of a greater dilution of antiserum and tracer, with delayed addition of tracer, resulted in a more sensitive assay suitable for measuring β‐thromboglobulin in urine. The use of a solid‐coupled antiserum under non‐equilibrium conditions allowed the measurement of plasma levels of β‐thromboglobulin after an assay incubation time of 1 h. These three radioimmunoassay systems for β‐thromboglobulin cover the likely clinical requirements for the measurement of this platelet specific protein.