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Method for evaluating quality of cultured neonatal pig Sertoli cells

Abstract:  Background:  Sertoli cells (SC) in the testis secrete factors that nourish and immunoprotect developing spermatozoa, which have made them the focus of studies that aim to generate localized tolerance, particularly for transplantation and perhaps autoimmunity. Several methods have been described to isolate these cells, which include a two‐step enzymatic digestion with limited assessment of the culture. Here we describe a one‐step method, and a series of tests for determining purity, viability, and function of the cultured cells.