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Water and alcohol extracts from Diaphragma Juglandis on anti-fatigue and antioxidative effects in vitro and vivo.

BACKGROUND
To estimate the anti-fatigue and antioxidative effects of water and alcohol extracts from Diaphragma juglandis (DJ), H2 O2 -treated HepG2 cells were used as an in vitro model to determine the total antioxidant capacities of these two extracts, and behavioral tests on mice and biochemical assay were performed in vivo experiments.


RESULTS
The results indicate that both extracts possess remarkable HepG2 protective capacities and were capable of scavenging DPPH and ABTS as Vc. Besides, they could significantly prolong the bar climbing time and force swimming time, decrease the serum urea nitrogen and increase the lactate dehydrogenase level and glycogen content. These extracts could also improve the activities of total antioxidant capacity, malondialdehyde, superoxide dismutase, catalase and glutathione peroxidase.


CONCLUSION
As a conclusion, both water and alcohol extracts from DJ showed good performance on anti-fatigue and could be potential antioxidant additive in the field of functional food. This article is protected by copyright. All rights reserved.