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Response of Apple Buds Cultured in Vitro to Abscisic Add


Abscisic acid (ABA) inhibited bud break and shoot elongation in seedling stem explants of apple (Malus domestica Borkh cv. Northern Spy) cultured in vitro. Inhibition was complete in culture medium containing 100 μM ABA. Transfer of buds from ABA-containing medium to basal medium resulted in increases in both bud break and shoot elongation. ABA levels in such buds declined rapidly following transfer, and growth began when ABA concentration in the buds dropped below a threshold value.