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A rapid, routine atomic absorption spectrometry method for the determination of selenium at sub-microgram levels in animal tissue.

Abstract A method is reported for determining selenium (Se) in animal tissue through Schoniger combustion, Se reduction to H2Se, and measurement by flame atomic absorption. The method provides several improvements over present hydrogen selenide generation apparatus and procedure. For the National Bureau of Standards Bovine Liver reference material containing 1.1±0.1 ppm Se, recovery averaged 100% with a relative standard deviation of 15 %. The minimum detecable quantity is 0.04 Ng Se. Data from the literature and results of analyses in our laboratory indicate that the method is adequately sensitive for tissue samples of almost any origin. Overall, our method is sensitive, precise, accurate, and simple and fast enough to use economically in routine monitoring of wildlife samples.