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Improved Detection of Streptococcus pneumoniae in Middle-Ear Fluid Cultures by Use of a Gentamicin-Containing Medium

ABSTRACT The performance of Columbia agar medium with added sheep blood and gentamicin (CAG) for isolation of Streptococcus pneumoniaefrom middle-ear fluid cultures was compared to that of routine blood agar medium (BA). Of 238 pneumococcal isolates recovered, CAG plates detected 233 (97.9%) but BA plates detected only 208 (87.4%) (P < 0.001).