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[Anorexia nervosa with regard to oligonucleotide microarray technique--own data].

AIM
The aim of the study was to discover the transcript expression profile of selected genes coding receptors of leptin and orexin A and B by using the oligonucleotide microarray technique (Affymetrix, HG-U133A) in patients who suffered from anorexia nervosa (AN).


METHOD
The peripheral blood of mononuclear cells (PBMC) of four AN patients complying with the ICD-10 and the AN diagnostic criteria DSM IV were analysed. Two patients suffered from the restricting type of AN (AN-R) and two suffered from the binge-eating/ purging type of AN (AN-BP). Four patients were our reference group and they did not suffer from eating disorders. The material used in the assay was the total RNA which was isolated from the PBMC of patients. The total RNA was used to investigate the transcript expression profile of selected genes by using the oligonucleotide microarray technique (Affymetrix, HG-U133A). For six and for eight oligonucleotide microarrays, the accumulation analysis method was used (clustering, Cluster v 3.0) to analyse the results.


RESULTS
Hierarchical clustering resulted in separate clusters for patients who suffered from AN-R, AN-BP and patients from the reference group.


CONCLUSIONS
Taking into consideration the hierarchical clustering for six and for eight oligonucleotide microarray performing different transcript expression profile of selected genes coding orexigenic peptides (OXA and OXB) and anorexigenic peptides (LEP) we suggest that the oligonucleotide microarray method differentiates two type of anorexia nervosa: the restricting type of AN (AN-R) and the binge-eating/purging type (AN-BP).