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Comparison of Carnoy’s Solution and 96% Ethyl Alcohol Fixation in Bloody Pap Smears

OBJECTIVE
To compare 2 methods of fixation in bloody Pap smears with Carnoy's solution and 96% ethyl alcohol.


STUDY DESIGN
After observation of contact bleeding, 2 samples were prepared from cervical cells with conventional Pap smear. One sample was fixed in 96% ethyl alcohol and another sample was fixed in Carnoy's solution.


RESULTS
Of 450 slides, 410 were selected for study. In study of cell adequacy, diagnosis of squamous cells and glandular cells was better in Carnoy's-fixed slides. Blood contamination of slides was reduced in Carnoy's-fixed slides (13.85% vs. 49.51%), and clearance of slides was increased in Carnoy's-fixed slides. Diagnosis of inflammatory cells and pathogenic microorganisms in was increased in Carnoy's-fixed slides, but no difference was seen in diagnosis of epithelial cell and glandular cell abnormalities.


CONCLUSION
Carnoy's solution can be used as an effective fixative in bloody smears in conventional Pap tests.