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Computational structural biology

target. We have recently shown that very limited spatial restraints from paramagnetic NMR, chemical crosslinking or small angle light scattering experiments can be sufficient to determine the structure of protein complexes if the structures of the individual components are known. The individual structures of the 13 subunits of the E.coli DNA replisome have been solved by X-ray crystallography, however, the highly dynamic nature of the molecular machinery makes structural studies of the full complex difficult. The project will use bioinformatics to assemble the core complex with the help of sparse experimental data that was collected in our laboratory and by collaborators.