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“Genetic Profiling of Breast Cancer using cDNA Microarray.”

Abstract: In defining the grade, stage, and residual determination of Breast Cancer and its subtypes, the molecular basis of analysis has become increasingly important. Vast-scale research studies have discovered a large number of genetic changes that cause the development and progression of breast cancer subtypes. Microarray and biotechnological approaches of hybridizationbased analysis and microtiter level evaluation have come a long way in determining the expression level of mutant or hereditary genes. For Genomic Research and Diagnostics, DNA Microarray analysis allows for the simultaneous investigation of thousands of DNA sequences. Breast cancer subtypes are distinguished by clusters of co-expressed genes discovered by the manipulation of mammary epithelial cells. The efficacy of a treatment is determined by the prognostic development of an individual patient. A comparison of gene expression levels in the Pre-treatment and Post-treatment stages has evolved into a concept that has the potential to change genetic dissection, drug development, disease diagnostics, and individualised therapeutic solutions. The goal of this review was to look into how to diagnose breast carcinomas using changes in gene expression patterns derived from cDNA microarrays and how to link tumour characteristics to clinical outcomes. Keywords: Breast Cancer, Genes, Mutations, Gene Expression, DNA Microarray, Microarray-Analysis