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Phenobarbital induction of CYP6D1 is due to a
 trans
 acting factor on autosome 2 in house flies,
 Musca domestica

To improve our understanding of phenobarbital (PB) mediated induction of CYP6D1 we examined the genetic linkage of PB induction using PB responsive (aabys) and non‐responsive (LPR) strains of house flies. PB induction was linked to autosome 2, indicating that CYP6D1 is trans regulated by this PB inducible element. Our results are discussed relative to a previous hypothesis that the same regulatory genes are responsible for both induction and monooxygenase‐mediated insecticide resistance.