{'fraction_non_alphanumeric': 0.04250720461095101, 'fraction_numerical': 0.020172910662824207, 'mean_word_length': 5.55188679245283, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Vaginal irritation of acetic acid thermosensitive in situ gel in mini-pigs

Objective:To evaluate the vaginal irritation of acetic acid thermosensitive in situ gel(AcidTISG) in mini-pigs.Methods:Nine female mini-pigs were randomly divided into three groups.Each pig was vaginally administrated with 5 m L of blank gel(control),Acid-TISG or commercial nonoxynol gel(N-9 jelly),once daily for 7 days.Cervicovaginal lavage(CVL) fluids were collected before administration,and 24 h and 72 h after the last administration.The number of inflammatory cells and neutrophilic granulocytes in the CVL was detected by flow cytometry.The difference between three groups was compared.Mini-pigs were sacrificed 72 h after the last administration.The vaginal tissues were observed.CD45 expression in the vaginal mucosa was semi-quantitated by immunohistochemical assay.Results:The number of inflammatory cells and neutrophilic granulocytes was significantly lower in Acid-TISG group than in N-9 jelly group(P 0.001).However,there was no significant difference between Acid-TISG gel and control groups(P 0.05).Compared to control group,CD45 expression in vaginal mucosa was not significantly different in Acid-TISG group(P 0.05),but significantly increased in N-9 jelly group(P 0.05).Conclusion:Vaginal irritation of Acid-TISG in min-pigs is mild.Acid-TISG has a good prospect as a topical contraceptive.