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Development of the quantitative test methods to determine the number of flavonoids in flowering shoots of semi-soft hawthorn

Flowers, fruits and shoots of various genus Hawthorn (Crataegus L.) species are used in domestic and foreign medical practice as cardiotonic agents. It is also possible to use hawthorn raw material for the prevention of cardiovascular system diseases among medical and pharmaceutical personnel. We consider semi-soft hawthorn Crataegus submollis Sarg. to be a prospective raw material species as its flowering shoots are chemically similar to the red-haw hawthorn flowers. We developed the quantitative test method to determine the number of flavonoids expressed as hyperoside in flowering shoots of semi-soft hawthorn using differential spectrophotometry with an analytical wavelength of 412 nm.