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Detection of lipoprotein abnormalities by a sensitive electrophoretic test system for the prevention of cardiovascular disease.

With the use of new and improved technology and methodology, an electrophoretic test system has been developed to determine serum lipoprotein patterns. The main features of this system, which distinguish it from other methods are: (1) high sensitivity and sharp resolution, which enable the identification in human serum of two beta, two pre-beta, and alpha lipoprotein fractions and additional subfractions; (2) rapid analysis of blood samples to demonstrate accurately the lipoprotein fractions as they circulate in the natural state in plasma; and (3) low cost of the test since the materials and supplies are inexpensive. The application of the test system to the analysis of serum samples from normal individuals and patients with defined pathologic conditions resulted in the following new developments: (1) the demonstration of abnormal lipoprotein patterns that aid the diagnosis of clinical disorders of lipid metabolism; and (2) the detection of abnormal lipoprotein fractions associated with atherosclerotic cardiovascular diseases (ACD). The determination of serum lipoproteins is an indispensable part of the evaluation of an individual for ACD because they are the native molecules in the circulation which are intimately associated with the blood vessels that become atherosclerotic.