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The N‐terminal amino acid of apolipoprotein D is putatively covalently bound to 3‐hydroxy‐3‐methyl hexanoic acid, a key odour compound in axillary sweat

Axillary sweat is odourless when freshly collected at the surface of human skin, but it contains non‐odoriferous precursors, which can be transformed into odorous substances by bacteria. E‐3‐methyl‐2‐hexanoic acid (3M2H) is one of the key odorous substances, but there are two contradictory reports about its precursor form. One report states that 3M2H linked non‐covalently to apolipoprotein D (apoD) is the precursor, while a second report states that 3M2H–Gln identified in human axillary sweat is the precursor. Recently, 3‐hydroxy‐3‐methyl hexanoic acid (HMHA) and 3‐methyl‐3‐sulfanylhexane‐1‐ol (3M3T) have also been identified and reported as characteristic components found in apocrine sweat. To better understand the formation of axillary odours and the structural relationships between these compounds and apoD, we characterized the linkage between odorous substances and apoD in human axillary secretions. ApoD was purified from human axillary secretions collected from 50 healthy female volunteers and was then digested by trypsin and analysed by MALDI‐TOF mass spectrometry. A Mascot search showed that 8 peaks identified in the trypsin‐digested samples correspond to the masses calculated for theoretically digested apoD sequences and the purified protein was assigned as a precursor of apoD [Homo sapiens]. One spectrum corresponded to the theoretical peak of HMHA linked covalently to the N‐terminal fragment of apoD. In contrast, no spectrum corresponded to the theoretical peak of a 3M2H adduct or to an unmodified N‐terminal fragment of apoD. These results indicate a possibility that HMHA binds covalently to the N‐terminal amino acid of apoD in human axillary secretions.