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Two-dimensional crystallization and preliminary electron crystallographic result of partially purified F0 from porcine mitochondria

After removal of cytoplasmic sector F1 from submitochondrial particles of F0F1-ATP synthase complex with guanidine hydrochloride, the transmembrane sector F0 was specifically extracted from the stripped membranes in the presence of detergent CHAPS and partially purified. Two-dimensional crystals were produced by the reconstitution of the partially purified F0 into asolectin and microdialysis. The obtained crystals are able to diffract to 2 nm. The projection map of the negatively stained crystal shows that the crystal has p4212 symmetry, lattice constant, a=b=14.4 nm. A unit cell contains four F0 molecules.