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[Identification and study on effect of new stress protein from Neisseria gonorrhoeae].

OBJECTIVE
To investigate the influence of changing environmental growth conditions on the virulence of Neisseria gonorrhoeae.


METHODS
The gonococci were subjected to 1 mmol/L hydrogen peroxide (sublethol concentration) for one hour.


RESULTS
A novel gonococcal stress protein with molecular weight of 62,000 was identified by radioactive labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Gonococci exposed to 1.0 mmol/L hydrogen peroxide made it significantly more resistant to higher concentrations of hydrogen peroxide and to neutrophils than control organisms (P < 0.01).


CONCLUSIONS
These results suggest that the 62,000 stress protein might increase the virulence of N. gonorrhoeae and might be an adapted regular protein produced by N. gonorrhoeae survived within human neutrophils.