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RAPID IN SITU HARVESTING AND CYTOGENETIC ANALYSIS OF PERINATAL TISSUE SAMPLES

Thirty perinatal solid tissue samples were used in a pilot study to test the efficacy of collagenase disaggregation onto coverslips, open system culture under low O2 conditions, pre‐harvest incubation in bromodeoxyuridine (BrdU) and colcemid, and in situ automated harvesting. Following the success of the pilot study, the new method was applied to a further 126 consecutive diagnostic samples making a total of 156. The method reduced average tissue culture times from 17 to 8·7 days (range 2–17), improved success rates from 76 to 88 per cent, and simultaneously increased the resolution of cytogenetic analysis.