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Applications of Differential Spectrophotometry in Analytical Chemistry

Differential spectroscopy is an elegant and powerful analytical method, based on the relatively simple principles of classical absorption spectroscopy. Some background to differential spectroscopy as well as an updated applications in the period 2000–2013 are reviewed. Associated with chemometrics, it can become a powerful tool for the pharmaceutical industry. Indeed, it is suitable for analysis of solid, liquid, and biotechnological pharmaceutical forms. This review focuses on applications used for qualitative and quantitative analysis. Moreover, it can be implemented during pharmaceutical development, in production for process monitoring, and in quality control laboratories.