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A 43Ca and 31P NMR study of the calcium and phosphate equilibria in heated milk solutions

Summary Casein micelle suspensions, colloidal Ca phosphate-free casein solution and simulated milk ultrafiltrate (SMUF) were studied using high-resolution 43Ca and 31P NMR in the temperature range 4–64 °C. Only one 43Ca and one 31P signal was obtained for each solution. Signal intensities and line widths varied with the environment and were affected by the redistribution of Ca and P during heating and cooling. Based upon the temperature-dependent broadening of the 43Ca signal, five different Ca environments were discerned in the heated milk fractions. The Ca state, induced by heating of a casein-containing milk salt solution, differed from both the state of Ca present in micellar colloidal Ca phosphate and from the state of Ca induced by the heating of SMUF.