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Determining the digestible lysine requirement of 22 to 47 week-old Lohmann laying hens using two requirement titration methodologies

A large laying hen trial was conducted to determine the digestible Lysine (Dig Lys) requirement of laying hens using increasing crude protein (CP) or constant CP Lys titration methodologies from 22 to 47 wk. In total, 1,680 Lohmann LS Lite caged layers (22 wk of age) were allotted to 15 dietary treatments, and each treatment had 8 replications of 14 hens. The first 7 experimental diets (increasing CP series) contained Dig Lys levels increasing from 0.56 to 0.92% with respective protein levels increasing from 13.8 to 21.7%. The second series of experimental diets (Trts 8–14) contained the same Dig Lys levels as Trts 1 to 7, but the CP was kept constant at approximately 16%. Dietary treatment 15 was a control diet that was calculated to contain 18.6% CP and 0.81% Dig Lys. Increasing Dig Lys levels had a large effect on egg production, egg weight, egg mass, and feed efficiency. Increasing Dig Lys levels had a lesser effect on egg component measurements such as percentage of yolk, white, and solids. Broken line regression, quadratic regression, and the intercept of the 2 regressions (QBL) were used to estimate the Dig Lys requirement. Broken line regression yielded the lowest requirement and quadratic regression yielded the highest, with the QBL method yielding an intermediate requirement estimate. For egg mass, Dig Lys requirements were generally slightly in excess of 600, 800, and 700 mg/ hen/d for the broken line, quadratic, and QBL models, respectively, and the Dig Lys requirements did not differ greatly between the increasing and constant CP diet series.