{'fraction_non_alphanumeric': 0.03050595238095238, 'fraction_numerical': 0.006696428571428571, 'mean_word_length': 5.255813953488372, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Enriched Cholesterol Diet Increases Expression of Renal Epithelial Na+ Channel Gamma Subunit in Rats

The epithelial Na+ channel (ENaC) is a trimeric transmembrane channel expressed in multiple secretory organs. ENaC is responsible for regulation of Na+ transport and balance in the kidney with the gamma subunit (γENaC) primarily responsible for regulation. However, the impact of enriched cholesterol diet on the regulation of ENaC during insulin resistance is not well described. To examine the effects of enriched cholesterol diet and activation of the angiotensin receptor on renal ENaC protein during insulin resistance, Otsuka Long Evans Tokushima Fatty (OLETF) rats and their lean, non‐insulin resistant controls, Long Evans Tokushima Otsuka (LETO) rats were supplemented with an enriched cholesterol diet (ECD; 2% + 0.5% cholic acid) and/ or treated with an angiotensin receptor blocker (ARB; 10 mg olmesartan/kg/d) for 16 weeks. The renal ENaC alpha and gamma subunits were measured by Western blot. The results suggest that elevated dietary cholesterol is associated with a quicker increase in blood pressure and expression of the ENaC γ loop. The ENaC γ loop was suppressed in the 24‐week‐old OLETFs, further implicating that the cleavage of ENaC γ loop is involved in renal uptake of Na+ resulting in volume‐dependent hypertension.