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Determination of indole-3-carbinol and indole-3-acetonitrile in Brassica vegetables using high-performance liquid chromatography with fluorescence detection

A simple and sensitive method for determining indole-3-carbinol (I3C) and indole-3-acetonitrile (I3A) from Brassica vegetable was developed using high-performance liquid chromatography with fluorescence detection. The indolic glucosinolate as well as I3C and I3A concentrations were found to be significantly higher in Brassica rapa L. ssp. pekinensis than in B. Juncea var. integrifolia.