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Subunit composition of the pre‐T‐cell receptor complex analysed by monoclonal antibody against the pre‐T‐cell receptor α chain

The pre‐T‐cell receptor (TCR) complex, which consists of a heterodimer of the TCRβ‐chain and the pre‐TCRα‐chain, is known to regulate early thymocyte development. The pre‐TCR complex contains CD3 subunits as a signal‐transducing molecule, but the exact subunit composition of the fully assembled pre‐TCR complex remains to be elucidated. In particular, the association of the CD3ζ‐chain with the pre‐TCR is controversial. In the present study, we have generated a monoclonal antibody against the cytoplasmic portion of the pre‐TCRα‐chain, and analysed a subunit composition of the pre‐TCR complex. We demonstrated that the CD3ζ‐chain is physically associated with the pre‐TCR in immature T cells. Thus, the result strongly supports the previous findings that CD3ζ contributes to signalling mediated through the pre‐TCR complex.