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AB0103 Anti-inflammatory and immunomodulatory effects by human umbilical cord mesenchymal stem cell derived microvesicles in rats with collagen induced

Background Immunologic deranging and persistent inflammation is closely associated with the arising and developing of rheumatoid arthritis (RA). Increasing investigators demonstrated microvesicles (MVs) derived from mesenchymal stem cell (MSC-MVs) might simulate immune regulation and tissue repair of the parental cells. However,the immunotherapeutic potential of MVs in RA remains unknown. Objectives We investigated the therapeutic effects of MSC-MVs in RA model collagen induced arthritis (CIA) rats. Methods We tested the therapeutic effects of MSC-MVs on CIA rats,levels of T helper 17(Th17), regulatory T cell,and cytokines related,as well as specific transcriptional regulation factor Foxp3 and ROR-γT analysis was performed. Results Here, we show that MSC-MVs administration effectively improve arthritic symptoms, inhibit synovial hyperplasia,thus delaying the progression to inflammatory bone destruction, as effective as their original cells, exerting arthralprotective effects. MSC-MVs treating inhibited the proliferation of T cell,accelerated the apoptosis. MSC-MVs treating reduce proinflammatory cell Th17,cytokines IL-17, while a incline in the level of an-inflammatory cell Treg,ncytokines TGF-β. In the spllen and ankle joint of CIA rats, MSC-MVs treating increased the expression level of Foxp3 and coinciding with the ROR-γT suppressed, which, the enhanced therapeutic effects correlated with the increase of dosage in a certain range. Conclusions MSC-MVs showing anti-inflammatory and immunomodulatory activities on CIA rats, suggesting a new and feasible strategy for protection against RA. References E.M. Gravallese, S.R. Goldring, Cellular mechanisms and the role of cytokines in bone erosions in rheumatoid arthritis, Arthritis Rheum. 43 (10) (2000) 2143–2151. S. Culshaw, I.B. McInnes, F.Y. Liew, What can the periodontal community learn from the pathophysiology of rheumatoid arthritis? J. Clin. Periodontol. 38(Suppl. 11) (2011) 106–113. Schett, G., D. Elewaut, I.B. McInnes, J.M. Dayer, and M.F. Neurath, How cytokine networks fuel inflammation: Toward a cytokine-based disease taxonomy. Nat Med, 2013. 19(7): p. 822–4. Disclosure of Interest None declared