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Neuronal Nogo‐A in New‐born Retinal Ganglion Cells: Implication for the Formation of the Age‐related Fiber Order in the Optic Tract

Nogo‐A is highly expressed in oligodendrocytes in the adult central nervous system (CNS). Recently it was found that Nogo‐A is also expressed in some neuronal types during development. Here, we examined the expression pattern of Nogo‐A in both the retina and optic tract (OT) of mouse embryos from E12 to E15. After perturbation of its function in the OT for 5 hr in the brain slice culture system using a Nogo‐A specific antibody or antagonist of its receptor (NEP1‐40), the optic nerve fibers and growth cones were traced with DiI. We showed that most Tuj‐1 positive new‐born neurons at E12 were Nogo‐A positive. At E15, retinal neurons reduced the Nogo‐A expression. It was worth noting that some projecting axons expressed Nogo‐A along the retinofugal pathway. On the basis of their specific locations within the superficial half of the OT and the colocalization with GAP‐43 (a marker for the newly born growth cones and axons), we concluded that those Nogo‐A positive axons were the newly arrived retinal fibers. Blocking the function of Nogo‐A with Nogo‐A antibody or NEP1‐40 resulted in the shift of DiI labeled axons and growth cones from the superficial half to the whole depth of the OT. These results indicate that Nogo‐A in the newly born retinal ganglion cells (RGCs) and their axons are involved in sorting out the newly arrived axons to the subpial region of the OT. Anat Rec, 299:1027–1036, 2016. © 2016 Wiley Periodicals, Inc.