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Reactivity of Antigens Derived from Different Structural Genes of SARS-CoV to Convalescent Sera

Objective To study the reactivity of recombinant and synthetic antigens derived from different structural genes of SARS-Coronavirus toward convalescent sera of SARS patients (as a probe into the humoral immune response induced by the virus).Methods Multi-epitope chimeric recombinant SARS-CoV "Spike" protein and "Nucleocapsid" protein antigens and synthetic E M peptide antigens were constructed using epitope prediction software to scan the published SARS-CoV genomic sequence.Purified antigens were used to coat microplates and presence of corresponding antibodies in convalescent sera was detected using an indirect ELISA format.Results The number of positive reactive sera over the number of convalescent sera tested using the recombinant S and N antigen of SARS-CoV and E and M peptide antigens were as follows:"S" 33/46(71.7%),"N"41/46(89.13%),"E"14/46(30.43%) and"M"24/46(52.17%).Conclusion There exist quite marked differences among antibody responses in SARS convalescent sera when tested with recombinant or synthetic antigens derived from different structural genes.