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Intracellular proliferation of clover yellow vein virus is unaffected by the recessive resistance gene cyv1 of Pisum sativum

The pea cyv1 gene is a yet‐to‐be‐identified recessive resistance gene that inhibits the infection of clover yellow vein virus (ClYVV). Previous studies confirmed that the cell‐to‐cell movement of ClYVV is inhibited in cyv1‐carrying pea plants; however, the effect of cyv1 on viral replication remains unknown. In this study, we developed a new pea protoplast transfection method to investigate ClYVV propagation at the single‐cell level. Using this method, we revealed that ClYVV accumulates to similar levels in both ClYVV‐susceptible and cyv1‐carrying pea protoplasts. Thus, the cyv1‐mediated resistance would not suppress intracellular ClYVV replication.