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Development of a continuous cell line from the sand fly Lutzomyia longipalpis (Diptera: Psychodidae), and its susceptibility to infection with arboviruses.

A new cell line (LL-5) developed from eggs of the phlebotomine sand fly Ll is described. The culture consists of at least 2 distinct cell types, which are epithelioicl and fibroblastoid in character. The cells are grown in MM/VP12 medium and form monolayers on glass or plastic containers. The origin and identity of the LL-5 cells were confirmed by isozyme analyses. Susceptibility of the cells to 29 arboviruses was tested. The viruses examined included representatives of the genera Vesiculovirus, Orbivirus, Flavivirus, Alphavirus, Bunyavirus , and Phlebovirus . Only 13 of these viruses multiplied in the LL-5 cells. Surprisingly, most of the phleboviruses did not replicate. Changuinola virus produced moderate cytopathic effect in the L. longipalpis cells and induced a persistent infection. This is the first report of a continuous sandfly cell culture.