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Studies on the template activity of 'isolated' Xenopus erythrocyte nuclei. I. The effects of ions.

The isolation and characterization of nuclei from Xenopus erythrocytes is described. Such nuclei are freely permeable to ions and large molecules. They have an extremely low basal rate of RNA synthesis which can be increased by the addition of Escherichia coli RNA polymerase and by alteration in the ionic strength of the incubation medium within physiological limits. The results are discussed in terms of the mechanisms which control gene activity in these cells.