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Evaluation of Tilmicosin Contamination in Eggs Following Its Administration to Laying Hens and Subsequent Assessment of Dietary Risks to Chinese Consumers.

In the present study, we analyzed the tilmicosin residues in eggs as well as the dietary risks posed by the residual drug to Chinese consumers. Tilmicosin was administrated to laying hens via drinking water in doses of 100 mg/L and 300 mg/L (dose 1 and dose 2) for 5 days. Its residues distribution within egg matrices were detected using a QuEChERS method coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results indicated that the tilmicosin residue was the highest in the whole eggs and yolks on day 2 after medication and in the whites on day 5 of drug administration for dose 1 and dose 2 with the residues (µg/kg) being the following: 73.4 and 444.3 in the whole eggs; 152.0 and 1141.1 in the yolks; and 48.8 and 277.6 in the whites, respectively. During withdrawal phase, the tilmicosin levels declined to less than the limit of detection (LOD) after 29 days and 49 days for the whole eggs, after 24 days and 44 days for the yolks, and after 19 days and 49 days for the whites for doses 1 and 2, respectively. An assessment of the dietary risk for Chinese consumers revealed that the hazard quotients (HQ) values for tilmicosin consumption were less than 1 for dose 1 and greater than 1 for dose 2, with children (2 to 7 years) and adult males (over 65 years) being the high-risk groups. These results suggest that the possible risk associated with tilmicosin contamination in eggs should not be ignored since the accidental or occasional misuse of tilmicosin in the case of egg-laying hens may occur from time to time, particularly on small farms. PRACTICAL APPLICATION: The modified QuEChERS method was used to analyze the tilmicosin residues in egg matrices and a subsequent dietary exposure assessment for Chinese consumers was performed, which can serve as a reference for the food safety risk posed by antibiotic misuse in egg layers.