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A Method for the Quantitation of Immunoglobulin Glass-Specific Antibodies 1

Summary A new method for the quantitative determination of antibodies according to the immunoglobulin class to which they belong has been described. It consists of two parts: (a) the removal of all antibody in the serum or secretions by absorption with excess antigen; and (b) the quantitation of a single immunoglobulin class of antibody bound to the sedimented antigen by means of a competitive-inhibition radioimmunoassay. The method is specific, quantitative, and sensitive to at least 1 μg/ml of specific antibody. The procedure is demonstrated by the quantitation of antibodies to sheep red blood cells according to immunoglobulin class in days 4 and 12 mouse antisera and by the detection of immunoglobulin class-specific antibodies to influenza virus in mouse lung washings.