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[The spectrum and dynamics of free amino acids in bovine ocular tissue and their interactions with pilocarpine. II. The aqueous humor, choroid epithelium and the choroid].

The authors revealed by two-dimensional ascendent chromatography on paper and thin-layer chromatography that a 3% aqueous solution of pilocarpine acts specifically on the free amino acids in the choroid, in the choroid epithelium and the aqueous humour of the bovine eye and that it reduces the total free amino acid level more in the choroid epithelium than in the aqueous humour. Specific changes of free amino acid levels caused by pilocarpine indicate the routes of transport, distribution and metabolic changes of pilocarpine and free amino acids. Based on these changes it is possible to evaluate also the physiological and morphological condition of ocular tissues and the organ of vision resp.