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A novel synthesis of iminodiacetic acid: Biocatalysis by whole Alcaligenes faecalis ZJB‐09133 cells from iminodiacetonitrile

Iminodiacetic acid (IDA) has been widely used as an important intermediate in the fine chemical industry. In this study, a novel synthesis route of IDA from iminodiacetonitrile by whole microorganisms was investigated. A strain with the capability of producing nitrilase, ZJB‐09133, was isolated and identified, and later named Alcaligenes faecalis ZJB‐09133. In addition, the detailed biocatalysis of iminodiacetonitrile to produce IDA using ZJB‐09133 was investigated. The results showed that the conversion reached 65.3% in Na2HPO4‐NaH2PO4 buffer of pH 8.0 under the following conditions: cells in the amount of 0.075‐g DCW/L, 1.5% substrate, conversion time of 8 h, and a reaction temperature of 35°C. To the best of our knowledge, this is the first time that the production of IDA using a biocatalysis method has been reported. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011