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Investigation of alopecia following sulphur rolling of mice

Watford Road, Harrow, HAl 3UJ The successful use of sulphur has been reported for the control of mange mites on laboratory mice (Green & Needham, 1974). Since the method was described, the colony has been treated several times, with some 150000 mice receiving treatment. A small number of these animals (less than 0·1 %) developed a degree of alopecia following treatment. This condition was first seen about 7 days after sulphur rolling and in most cases led to some 50 % hair loss. It was noticed that alopecia first appeared in a room where an excess of sulphur had been used for treatment, as judged by the presence of the chemical on the floor, cage tops and in the bedding. In view of the possible implications of alopecia on the maintenance of experimental mice it was decided to see whether this condition could be experimentally induced by the application of an excessive amount of sulphur. 18 adult male and 18 adult female CBA/H-T6 mice, issued from the Centre's Barrier Maintained (SPF) Breeding Unit were used. Groups of 6 male and 6 female mice were treated with sulphur, applied by hand and rubbed into the coat of the mice for 3 minutes. Similar groups of 6 mice of each sex received 2 treatments 48 hours apart while the 3rd group remained untreated. All mice were killed for examination by intraperitoneal administration of sodium pentobarbitone 7 days after the initial treatment. A snip of skin was removed from the neck, back and right hind leg, fixed in 10 %formol saline, embedded in paraffin wax and stained with Cole's haematoxylin and eosin. So that unbiased interpretation of the histological results could be made one author undertook the experimental sulphur treatment and coded the skin snips. The other author performed the histological examination unaware of the derivation of each individual specimen. Following reading the results were collated.