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Wnt Signaling Regulates the Lineage Differentiation Potential of Mouse Embryonic Stem Cells through Tcf3 Down-Regulation

Canonical Wnt signaling plays a rate-limiting role in regulating self-renewal and differentiation in mouse embryonic stem cells (ESCs). We have previously shown that mutation in the Apc (adenomatous polyposis coli) tumor suppressor gene constitutively activates Wnt signaling in ESCs and inhibits their capacity to differentiate towards ecto-, meso-, and endodermal lineages. However, the underlying molecular and cellular mechanisms through which Wnt regulates lineage differentiation in mouse ESCs remain to date largely unknown. To this aim, we have derived and studied the gene expression profiles of several Apc-mutant ESC lines encoding for different levels of Wnt signaling activation. We found that down-regulation of Tcf3, a member of the Tcf/Lef family and a key player in the control of self-renewal and pluripotency, represents a specific and primary response to Wnt activation in ESCs. Accordingly, rescuing Tcf3 expression partially restored the neural defects observed in Apc-mutant ESCs, suggesting that Tcf3 down-regulation is a necessary step towards Wnt-mediated suppression of neural differentiation. We found that Tcf3 down-regulation in the context of constitutively active Wnt signaling does not result from promoter DNA methylation but is likely to be caused by a plethora of mechanisms at both the RNA and protein level as shown by the observed decrease in activating histone marks (H3K4me3 and H3-acetylation) and the upregulation of miR-211, a novel Wnt-regulated microRNA that targets Tcf3 and attenuates early neural differentiation in mouse ESCs. Our data show for the first time that Wnt signaling down-regulates Tcf3 expression, possibly at both the transcriptional and post-transcriptional levels, and thus highlight a novel mechanism through which Wnt signaling inhibits neuro-ectodermal lineage differentiation in mouse embryonic stem cells.


Introduction
Embryonic stem cells (ESCs) are in vitro cultured cells derived from the preimplantation-stage embryo, which possess unconfined capacity for self-renewal and multi-lineage differentiation towards different embryonic germ layers. Pluripotency and self-renewal are two essential features of ESCs, which make them not only a very robust and suitable model for stem cell research, but also a promising source for regenerative medicine. Also, with the emergence of induced pluripotent stem cells (iPS) technology, understanding the basic mechanisms governing the embryonic stem state becomes of great interest for safe clinical applications in regenerative medicine and stem cell programming.
Among different signaling pathways, Wnt/b-catenin signaling has been shown to play a major role in maintaining self-renewal as well as in regulating ESCs differentiation [1,2,3,4,5,6]. The canonical Wnt/b-catenin signaling pathway is controlled by post-translational modifications of b-catenin leading to its differential protein stability and sub-cellular localization. In the absence of active Wnt signaling, b-catenin is negatively regulated by the socalled ''destruction complex'', consisting of the Apc and Axin scaffolding proteins and the glycogen synthase and casein kinases (GSK and CK1), resulting in proteolytic degradation and low levels of cytoplasmic b-catenin. Ligand-mediated Wnt signaling activation leads to nuclear translocation of b-catenin where it binds to members of the Tcf/Lef family of transcriptional factors thus modulating the expression of a broad spectrum of downstream target genes [7,8,9].
Although several studies have demonstrated the key role played by Wnt signaling in regulating self-renewal and differentiation of both mouse and human ESCs, the downstream effects through which Wnt exerts these functions have been a matter of controversy. To date, three models have been suggested in this regard: a. Tcf-independent, b-catenin/Oct4 signaling [25]; b. Tcf3 antagonism by nuclear b-catenin which relieves Tcf3 repression and enhances self-renewal. A minimal role for the canonical Tcf/b-catenin signaling has been suggested in this model [6]; and c. synergistic action of Tcf3 antagonism and the canonical b-catenin/Tcf1 signaling [5]. Although these studies have shed some light on the underlying mechanisms through which Wnt signaling controls self-renewal, none of the abovementioned models explains how this signaling pathway regulates the lineage differentiation potential of ESCs.
In order to elucidate the downstream effects of Wnt signaling on lineage commitment and differentiation in embryonic stem cells, we examined several Apc-mutant ESCs harboring different levels of Wnt signaling and compared their gene expression profiles with wild type ESCs. We show that activation of Wnt signaling downregulates Tcf3 expression in mouse ESCs. We provide evidence that Tcf3 down-regulation represents a main downstream effect through which Wnt signaling directs the differentiation of pluripotent ESCs towards non-neuroectodermal lineages. Moreover, we show that Wnt-mediated repression of Tcf3 involves epigenetic regulation associated with histone modifications and Wnt-mediated induction of miR-211. Our data demonstrate that Wnt signaling counteracts Tcf3 function at multiple levels, which ultimately ensures the delicate balance between self-renewal and differentiation in mouse ESCs.

Lineage differentiation in Apc-mutant ESCs correlates with the level of Wnt signaling
To attempt the elucidation of the mechanisms underlying lineage differentiation in the context of Wnt activation, we have derived several ES clones from pre-implantation blastocysts carrying different hypomorphic Apc alleles: Apc 1638T/1638T (ApcTT), Apc 1638N/1638T (ApcNT), Apc 1638N/1638N (ApcNN) [26,27], together with Apc +/+ as wild type controls. As previously reported, ApcTT, ApcNT, and ApcNN encode for a gradient of different Wnt signaling dosages [1,26], as also confirmed by TOP-Flash reporter assay [28] with ApcNN showing the highest Wnt activity (ApcNN&ApcNT.ApcTT.Apc +/+ )( Figure 1A). The potential of the Apc-mutant ES cells to differentiate into ecto-, meso-and endodermal lineages was also evaluated and confirmed by the teratoma formation assay followed by immunohistochemistry (IHC) analysis, matching our previous results obtained with ES clones obtained by two rounds of gene targeting by homologous recombination [1]. As expected, no expression of neuroectodermal markers (GFAP, SV2, and neurofilaments) was observed in teratomas derived from ApcNN ES cells ( Figure 1B).
ES cells can be cultured in serum-free medium supplemented with LIF, GSK inhibitor (CHIRON) and Mek inhibitor (PD), the so-called 2i medium [29]. Using the serum-free culture supplemented with a single inhibitor, we found that ApcNN cells have the highest colony-forming capacity when cultured in LIF+Mek inhibitor, suggesting that their constitutive Wnt signaling activity replaces the need for additional pathway activation by the GSK inhibitor ( Figure 1C). Of note, culturing ApcNN ESCs in medium supplemented with CHIRON reduced the colony formation capacity of these cells suggesting that a very high dosage of Wnt signaling can compromise the growth of ApcNN cells. We also observed that ApcTT and ApcNT cells formed similar number of colonies in different culture conditions independently of CHIRON supplementation, possibly pointing to the Wnt-independent effects of Apc mutations in these cells ( Figure 1C).

Wnt signaling down-regulates Tcf3 expression in mouse ESCs
To elucidate the molecular mechanisms underlying the altered cell fate decision in Apc-mutant ES cells, genome-wide transcriptional analysis was performed on the newly derived cells. Unsupervised hierarchical clustering analysis showed that global gene expression in ApcNN ESCs is already influenced before differentiation is induced, resolving ApcNN from WT expression profiles in different branches of the dendogram ( Figure 1D). Among the genes differentially expressed between ApcNN ES cells and their wild type counterparts (Table S1), we found that, unlike other pluripotency markers (e.g. Oct4, Nanog, and Sox2), Tcf3 was specifically down-regulated in ApcNN ES cells, an observation

Author Summary
The future successes of regenerative medicine largely rely on our knowledge of, and our capacity to manipulate, the cellular and molecular mechanisms governing stem cell differentiation. A growing body of evidence suggests that, in mouse embryonic stem cells, canonical Wnt/b-catenin signaling not only enhances self-renewal but also directs the cell fate decision towards non-neuroectodermal lineages. However, little is known about the mechanisms underlying the differentiation defects caused by constitutive active Wnt signaling. Using a set of Apc-mutant ESCs harbouring different levels of Wnt signaling, we found that, among others, down-regulation of Tcf3, a key member of the pluripotency circuit, as well as induction of a novel Wnt-regulated microRNA, miR-211, represent two important downstream effects through which Wnt signaling inhibits neural differentiation in mouse ESCs. We also provide a more detailed picture on how Wnt signaling counteracts Tcf3 function in stem cells by showing that Tcf3 repression, in the context of active Wnt signaling, involves histone modifications at the Tcf3 promoter and the activation of miR-211, which post-transcriptionally stabilizes Tcf3 downregulation. Understanding the downstream effects of Wnt signaling in ESCs is of both fundamental and translational relevance, as it may be exploited to manipulate ESC differentiation towards specific cell lineages.
which was further confirmed by qRT-PCR and western blot analysis (Figure 2A and 2B; and Figure S1). Further qRT-PCR analysis revealed that the observed downregulation is specific for Tcf3 but not for other members of the Tcf/Lef family ( Figure  S2A). Whereas Tcf3 was down-regulated in both ApcNN and Apc Min/Min ESCs, the latter encode for the most severely truncated Apc mutant allele and therefore for a very high level of Wnt signaling, other members of the Tcf/Lef family were exclusively up-regulated in Apc Min/Min ESCs.
Accordingly, Wnt activation achieved in wild type cells either by Wnt3a conditioned medium or by a GSK3-small molecule inhibitor (SB-216763), confirmed that Tcf3 down-regulation is a specific response to canonical Wnt signaling in mouse ESCs ( Figure 2C, 2D, and 2E, and Figure S2B and S2C). Moreover, using a gradient of the GSK inhibitor SB-216763, we observed that unlike the canonical Wnt targets Axin2 and Cdx1, downregulation of Tcf3 required a higher Wnt signaling level, possibly explaining why Tcf3 downregulation is observed in ApcNN cells but not ApcTT or ApcNT ESCs ( Figure 2F and Figure S1).

Rescuing Tcf3 expression in ApcNN ESCs partially restores neural differentiation
It has been previously shown that Tcf3 not only functions as a controller of self-renewal in wild type ESCs, but it is also required for proper neurogenesis in zebrafish, xenopus and mice [11,16,21]. We therefore hypothesized that Tcf3 down-regulation in ApcNN ESCs might mediate the neural differentiation defects observed in these cells. To test this hypothesis, we rescued Tcf3 expression by stably over-expressing its full-length cDNA in ApcNN ES cells ( Figure 3A, 3B). Tcf3 over-expression decreased TOP-Flash reporter activity ( Figure 3C) and, accordingly, reduced the transcript levels of Cdx1 and Brachyury (T), two well-known Wnt downstream targets. Gene expression profiling of Tcf3-expressing ApcNN cells confirmed that Tcf3 effectively reverses the expression pattern of several genes differentially expressed in ApcNN when compared to wild type ESCs ( Figure S3).
Since it has been previously reported that Tcf3 over-expression in wild type ESCs induces differentiation under self-renewing conditions [5], we first assessed whether over expressing Tcf3 in ApcNN ESCs induces similar effects in these cells. As reported above, ApcNN cells can grow in 1i medium (i.e. in LIF+Mek inhibitor) in the absence of GSK inhibitor ( Figure 1C). To investigate whether Tcf3 can restore their dependency on the GSK inhibitor in serum-free culture, Tcf3-over expressing ApcNN cells were seeded at clonal density under different conditions and subsequently stained for alkaline phosphatase (AP) to evaluate the percentage of undifferentiated colonies. We found that, similar to the parental ApcNN cells, Tcf3-rescued clones show the highest  Table summarizing the results obtained by teratoma differentiation assay from different Apc-mutant ESCs and their wild type controls. Tissue sections were stained with hematoxylin and eosin (H&E) or used in immunohistochemical analysis using specific antibodies against the neural markers: GFAP, neurofilaments and synaptic vesicles. Adult myosin was used as a mesodermal marker to stain the striated muscle differentiation. Cartilage differentiation was assessed either by H&E or theonin staining. Two independent clones were used for each genotype and differentiation was scored as: (2)  colony forming capacity in the presence of LIF and Mek inhibitor ( Figure 3D). Moreover, by applying the short term differentiation assay in N2B27 medium, we found that both ApcNN and their Tcf3-rescued counterparts retain expression of the pluripotency markers Nanog while fail to express the early differentiation markers Fgf5 ( Figure 3E). Hence, constitutive Wnt signaling prevents differentiation in a short-term assay despite the ectopic Tcf3 expression.
We then asked whether rescuing Tcf3 expression in ApcNN cells could affect the neural differentiation potential of these cells. To this aim, we applied the in vitro neural differentiation assay previously described by Bibel et al. [30]. We found that, whereas wild type ESCs readily gave rise to Tuj1-positive cells, no staining could be detected in ApcNN cells, while only few dispersed Tuj1expressing cells were observed in the Tcf3 rescued clones ( Figure 3F). In contrast, a clear increase in Nestin expression was observed in Tcf3 over-expressing cells ( Figure 3G and Figure S4). This suggests that, although Tcf3 could not restore the formation of fully mature Tuj1-proficient neurons, it does affect neural differentiation in vitro in a more subtle fashion towards neural Actb was used as an internal control; bars represent n = 2 6 SD. B. Western blot analysis of the core pluripotency markers Oct4, Nanog, Sox2 and Tcf3 on protein lysates isolated from two independent ApcNN clones and wild type control ESCs. Actb and Tubulin were used as an internal control. C-D. qRT-PCR analysis of Tcf3 in wild type ESCs treated for different time intervals with Wnt3a conditioned medium (C), or with the GSK-inhibitor SB-216763 (D). L-medium and DMSO were employed as controls, respectively. Actb was used as an internal control; bars represent n = 2 6 SD. E. Time course western blot analysis of Tcf3 expression in wild type ESCs treated with Wnt3a conditioned medium (Wnt3a CM) or control L-medium (LM). Actb was used as an internal control. F. qRT-PCR analysis of Tcf3 and Wnt target genes Axin2 and Cdx1 in wild type ESC treated for 48 h with different concentrations of GSK-inhibitor SB-216763 or DMSO as control. Actb was used as an internal control; bars represent n = 2 6 SD. doi:10.1371/journal.pgen.1003424.g002 progenitor-like cells. Next, we examined the differentiation potential of Tcf3-rescued ES cells in vivo by teratoma assay. We injected the newly generated clones into recipient isogenic mice to generate teratomas and analyzed them for the expression of different neuroectodermal markers by IHC. Interestingly, in contrast to the control ApcNN teratomas which did not express any neuroectodermal marker (0/20 analyzed teratomas), approximately 50% of all teratomas generated from different Tcf3 overexpressing ES clones were positive for the same set of markers (6/ 10, 6/10, and 4/10 teratomas originated from clones 1, 2 and 3, respectively)( Figure 4). However, the extent of neural differentiation was lower compared to teratomas originated from wild type ESCs. Unlike neuroectodermal lineages, Tcf3 did not rescue the mesodermal cartilage-differentiation defect.
The observed difference in the results obtained by in vivo and in vitro differentiation assay might reflect the presence of different microenvironmental factors and the longer period of differentiation in vivo, which result in a larger extent of neural differentiation in teratomas.
Overall, these results indicate that Tcf3 expression in ApcNN cells can partially rescue the neural differentiation defect characteristic of these cells. Next, we then asked whether Tcf3 down-regulation in wild type embryonic stem cells is sufficient to induce neural differentiation defects, characteristic of Wnt-high ESCs. To this aim, teratomas were obtained by subcutaneous transplantation of Tcf3 2/2 ESCs [14] followed by IHC and qRT-PCR analysis of different neural markers. We observed reduced neural differentiation in Tcf3 2/2 teratomas when compared to wild type controls ( Figure 5). However, high expression of the pluripotency markers Oct4 and Nanog was also observed in Tcf3 2/2 teratomas ( Figure 5). IHC analysis of Oct4 also showed that Tcf3 2/2 teratomas are largely composed of undifferentiated, embryonic carcinoma (EC) -like cells, confirming their undifferentiated nature. This is in contrast with ApcNN teratomas where pluripotency markers were down-regulated. These results suggest that Tcf3 down-regulation in wild type ES cells is necessary but insufficient to fully inhibit neural differentiation, and that canonical Wnt signaling is still required for redirecting the differentiation towards non-neuroectodermal lineages.

Tcf3 down-regulation in ApcNN ESCs is associated with histone modifications
To elucidate the mechanisms underlying Wnt-driven repression of Tcf3 expression, we first analyzed its promoter activity in ApcNN and wild type ESCs to localize the responsible regulatory elements. We employed luciferase reporter constructs containing a 6.7 kb genomic fragment upstream of the mouse Tcf3 ATG translation start site as well as a series of different deletion constructs spanning 4.5 kb, 3.5 kb, 2.2 kb, 1.2 kb and 176 bp fragments of the same region ( Figure S5A) [31]. The 4.5 kb fragment was previously shown to resemble endogenous Tcf3 expression in mouse embryo as well as embryonic derived neural stem cells [31]. To test whether Wnt signaling affects Tcf3 promoter activity, we transfected the different Tcf3 promoter constructs in ApcNN and wild type ESCs. Likewise, transfected wild type ESCs were also treated with Wnt3a conditioned medium or L-control medium to examine Tcf3 promoter activity. Using both approaches, we found that the Wnt-mediated repression of Tcf3 is not regulated by elements located within the 6.7 kb promoter region ( Figure S5A). However, we cannot exclude the possibility that long-range enhancer elements located outside the 6.7 kb promoter region might still contribute to the observed Tcf3 repression in Wnt context.
The mouse Tcf3 promoter contains a large CpG island extending over exon 1, 2 and 3. This indicates that DNA methylation may play a role in the regulation of Tcf3 expression [32]. To test whether the observed Tcf3 down-regulation in ApcNN ESCs results from DNA methylation, we employed the bisulfiteconversion method followed by sequencing and methylationspecific PCR to analyze the Tcf3 promoter in ApcNN cells and compare its methylation pattern to wild type ESCs. As depicted in Figure S5B, we found that similar to wild type ESCs, the Tcf3 promoter is unmethylated in ApcNN cells thus suggesting that DNA methylation is unlikely to represent the mechanism underlying Wnt-driven Tcf3 down-regulation in mouse ESCs.
Active and repressed promoters are thought to be associated with histone marks, which reflect the gene expression status of the corresponding genes. To test whether Tcf3 down-regulation in ApcNN cells is regulated via chromatin modifications, we performed chromatin immunoprecipitation (ChIP) to analyze post-translational histone modifications associated with active and repressed promoters. We studied the active-chromatin marks H3K4me3 and H3-acetylation as well as the repressed-chromatin marks H3K27me3 and H3K9me3 in the Tcf3 promoter and compared the histone modification patterns between ApcNN and wild type ESCs. The immunoprecipitated chromatin was then assessed by qPCR analysis with a panel of specific primers covering a region encompassed between 22 kb to +2 kb from the transcription start site (TSS), as well as 20 kb of the gene body within the Tcf3 locus. In accordance with the observed Tcf3 downregulation in ApcNN cells, we found a decrease in the activating marks H3K4me3 and H3Ac and, to a lesser extent, a slight increase in the repressive marks H3K27me3 and H3K9me3 ( Figure 6). Similarly, 12 h treatment of wild type ESCs with Wnt3a conditioned medium significantly reduced the H3Ac and H3K4me3 activating marks but had no effect on the H3K27me3 and H3K9me3 repressing marks ( Figure S6). These data demonstrate a correlation between Tcf3 expression and histone modifications in its promoter suggesting that Wnt signaling might regulate Tcf3 expression through epigenetic mechanisms. However, the mediator of this regulation still remains elusive.
miR-211, a novel Wnt-regulated microRNA, targets Tcf3 and attenuates early neural differentiation in wild-type ESCs It has been previously shown that members of the core pluripotency circuit are fine-tuned via microRNA-mediated regulation in embryonic stem cells [33,34,35,36,37]. Therefore we tested the idea whether Wnt-driven repression of Tcf3 expression might also be mediated, post-transcriptionally, by Wnt-induced miRNAs. To this aim, we profiled the different Apcmutant ESCs for microRNA expression by using a miRNA array immunofluorescence after 13 days of culture. G. Flow cytometric analysis showing expression of the neural progenitor marker Nestin in ApcNN ESCs stably expressing Tcf3 (Tcf3 OE) and their control cells (parental ApcNN clone and ApcNN transfected with the corresponding empty vector) or wild type ESCs. Cells were analyzed by the 24/+4 neural differentiation assay and stained with specific antibody against Nestin and Tuj1 after 13 days of culture. Wild type (WT) ESCs are shown as control to indicate the Tuj1 positive population which is absent in other genotypes (0.1% in average in Tcf3 OE clones). Numbers in the graph represent the percent of Nestin-positive cells. For wild type ESCs the Nestin-positive populations before and after excluding the mature neurons are shown. See also Figure S4 for defining different FACS gates. doi:10.1371/journal.pgen.1003424.g003 encompassing specific probes for all known mouse miRNAs [38] (data not shown). Of the different candidate miRNAs induced upon Wnt activation, mmu-miR-211 showed a Wnt dosagedependent up-regulation among the different Apc-mutant ESCs ( Figure 7A). Accordingly, activation of Wnt signaling in wild type ESCs either by Wnt3a conditioned medium (CM) or by GSK3 inhibition, confirmed that miR-211 is a novel Wnt-regulated microRNA in mouse embryonic stem cells ( Figure 7B and 7C).
In silico analysis with three software packages, namely Miranda [39], Targetscan [40] and PicTar [41], pointed to several potential miR-211 target genes predicted by all three programs. To narrow down the list of potential targets, qRT-PCR analysis was performed on wild type ESCs compared with ApcNN ( Figure S7A) as well as on wild type ESCs treated with Wnt3a CM ( Figure S7B). We excluded those predicted targets that showed up-regulation upon Wnt signaling. Based on these results Sox11, Sf3b1 and Tcf3 were selected for further analysis. Several stable ESC clones were generated which ectopically over-express miR-211 in an otherwise Figure 5. Tcf3 downregulation in wild-type ES cells impairs but does not fully inhibit neural differentiation. A. Immunohistochemistry analysis was used to evaluate the neural differentiation in teratoma samples derived from Tcf3 2/2 or their wild type control (GS1) ESCs. Immunostaining with specific antibodies revealed retention of the pluripotency marker Oct4 and expression of the neural markers GFAP, neurofilaments (2H3) and synaptic vesicles (SV2) in Tcf3 2/2 teratomas. Thionin staining was used to evaluate cartilage differentiation. B. RNAs were isolated from different teratoma samples and analyzed by qRT-PCR for differentiation markers. Dot plots show normalized qRT-PCR values for the neural markers Map2, b-III-Tubulin and GFAP and for the pluripotency markers Oct4 and Nanog among the different teratoma samples. Each dot represents one sample. doi:10.1371/journal.pgen.1003424.g005 wild type background ( Figure S7C). Western blot analysis showed that, unlike Sox11 and Sf3b1 ( Figure S7D), Tcf3 protein level was decreased upon miR-211 ectopic expression ( Figure 7D). To confirm that miR-211 directly targets Tcf3, we cloned the 39 untranslated region (39UTR) of the mouse Tcf3 gene in the pmirGLO reporter plasmid ( Figure 7E) and performed a luciferasebased reporter assay. Transfection of HEK293 cells with the Tcf3-39UTR reporter plasmid confirmed that Tcf3 is a direct target of miR-211 ( Figure 7F). The inhibitory effects of miR-211 were not observed when mutant forms of the 39UTR, i.e. lacking 7 or 4 nucleotides of the miRNA seed sequence target (MTR1 and MTR2 respectively) were used ( Figure 7F).
We next assessed the differentiation potential of miR-211 overexpressing clones using in vitro neural differentiation assay as well as in vivo teratoma formation. FACS analysis for Tuj1, a marker for mature neurons and Nestin, revealed that both miR-211 overexpressing ES cells and their wild type controls give rise to similar number of neurons and neural progenitor cells after 13 days of in vitro differentiation, thus suggesting that miR-211 does not affect terminal neural differentiation. As expected, ApcNN cells show a dramatic reduction in mature, Tuj1-proficient neurons ( Figure 7G). Teratoma formation assay also confirmed that miR-211 does not suffice to inhibit neural differentiation (data not shown).
To evaluate the role of miR-211 at earlier stages of differentiation, we derived embryoid bodies (EBs) from miR-211 overexpressing cells and their wild type controls and analyzed lineage differentiation at different time points. EBs derived from wild type ES cells encompass differentiated lineages from the three germ layers, thus providing an in vitro assay recapitulating the early steps of embryonic development. qRT-PCR analysis for different lineage-specific markers indicated that, unlike mesodermal, endodermal and pluripotency markers (data not shown), early neuroectodermal differentiation was specifically attenuated by miR-211. We found that expression of the primitive ectoderm marker Fgf5 and of the neural progenitor markers Nestin and Pax6 as well as the early neural differentiation marker Sfrp2 were repressed at day 3 of EB formation. Notably, these effects could not be detected at later time points (day 6, 9 or 12; data not shown). Similar results were obtained at early time points (i.e. after 24 h) in N2B27 culture medium, previously described to induce neural differentiation in mESCs [42] (Figure 7H and 7I). These results suggest that miR-211 functions at early stages of neural differentiation and its ectopic expression in wild type ES cells is not sufficient to inhibit further neural commitment as differentiation proceeds.
Altogether, our results indicate that miR-211, a novel Wntregulated miRNA, can fine-tune Tcf3 expression and attenuate early neural differentiation in wild type ESCs.

Discussion
The role of Wnt/b-catenin signaling in controlling self-renewal and lineage differentiation in pluripotent embryonic stem cells has Figure 6. Regulation of Tcf3 in ApcNN ESCs is associated with histone modifications. Schematic representation of mouse Tcf3 locus and the different amplicons (P1-P8) analyzed by QPCR in chromatin immunoprecipitation experiment. Chromatin was isolated from ApcNN and wild type ESCs and was immunoprecipitated with specific antibodies against the activating histone marks (H3K4me3 and H3Ac) and the repression histone marks (H3k27me3 and H3K9me3). The input DNA (chromatin before immunoprecipitation) and immunoprecipitated DNA was quantified by QPCR and using specific primers as described in materials and methods. Values from each amplicon were normalized to input chromatin and fold change was calculated relative to the corresponding negative region (P1). Bars represent n = 26SD. doi:10.1371/journal.pgen.1003424.g006 been a matter of controversy. Although both GSK3 inhibitors and Wnt ligands are essential to support ESCs self-renewal, it is yet unclear whether this occurs through b-cateninand TCFdependent mechanisms [43]. Among the members of the Tcf/ Lef family of transcription factors, Tcf3 and Tcf1 are the most abundant in ES cells. This is of relevance as, while Tcf1 appears to function as a canonical transcriptional activator upon association with b-catenin, Tcf3 acts as a b-catenin-independent transcriptional repressor of self-renewal, suppressing genes such as Nanog, Oct4 and other members of the core pluripotency circuitry [17,19]. In this scenario, it is yet unclear how canonical Wnt signaling controls the balance between Tcf1-and Tcf3-mediated gene activation and repression in the regulation of self-renewal and differentiation in ESCs.
During the last few months, several studies have been published on the specific roles of b-catenin and Tcf3 in these processes [4,5,6,44]. In the classical Wnt model, Tcf factors bind DNA and repress gene expression in the absence of active Wnt signaling. Activating the signaling pathway leads to the binding of b-catenin to Tcf proteins thus converting them from transcriptional repressors to transcriptional activators. Among the four members of Tcf/Lef family, Tcf3 seems to be different as its repressor function is not directly affected by Wnt signaling. In this perspective, two modes of action have been described for the relief of Tcf3 repression by Wnt signaling: 1) Tcf3 phosphorylation by homeodomain interacting protein kinase 2 (HIPK2) which is mediated by b-catenin and results in displacement of Tcf3 from its target sites [45]; and 2) direct physical interaction between bcatenin and Tcf3 which displaces Tcf3 and inhibits its repressive role in the context of active Wnt signaling [6,46]. Recently, using a knock-in mouse model lacking the b-catenin-interaction domain of Tcf3, Wu et al have demonstrated that counteracting Tcf3 function is not mediated by the physical interaction between bcatenin and Tcf3 during the first stages of embryonic development [47]. In view of these models, our data suggest that transcriptional and post-transcriptional down-regulation of Tcf3 expression might be yet another mechanism by which Wnt signaling inhibits Tcf3 function. It is worthwhile mentioning, however, that Wnt signaling does not seem to fully suppress Tcf3 expression and that residual levels of Tcf3 are retained even in the most severely truncated Apc mutant alleles (i.e. Apc Min/Min ESCs; Figure 2A) which encode for extremely high Wnt signaling dosages. Altogether these observations suggest that Wnt/b-catenin signaling regulates Tcf3 at several levels and by a combination of multiple mechanisms during different stages of embryonic development.
Although over-expression of a dominant negative form of Tcf1 or Tcf4 reduced the canonical Wnt reporter activity (TOP-Flash), it failed to rescue the neural differentiation in GSK-null ESCs [25]. Inhibition of b-catenin in GSK3b-null ESCs, however, was sufficient to rescue the neural differentiation defect thus confirming the central role of b-catenin-dependent mechanisms in this process [25]. The partial rescue of neural differentiation by Tcf3 expression in ApcNN cells, as shown here, highlights the distinct role of Tcf3 from other members of the Tcf/Lef family and suggests that a plethora of Tcf3-dependent and -independent mechanisms underlie the Wnt-regulated lineage differentiation in embryonic stem cells.
As for self-renewal maintenance in ES cells, Wray et al have shown that a mutant form of b-catenin where the trans-activating domain was deleted, can still maintain self-renewal in mESCs cultured in 2i medium [6]. This suggests that maintenance of selfrenewal is mediated by Tcf3 displacement rather than b-catenin signaling in 2i culture. Based on this, one can hypothesize that forced overexpression of Tcf3 in Wnt context could restore the dependency on CHIRON in serum-free culture. Our data show that Tcf3 overexpression in ApcNN cells does not induce differentiation in 2i culture, highlighting the dominant role of Wnt signaling in this process. This is in line with the report by Yi et al. which showed that over expressing Tcf3 in the context of Wnt signaling activation has minimal effect on self-renewal suggestive of a synergistic action of Tcf3 antagonism and bcatenin/Tcf1 signaling [5].
In an attempt to elucidate the mechanisms underlying Tcf3 downregulation in the context of active Wnt signaling, we found that Tcf3 down-regulation does not require DNA methylation but is associated with alterations in histone marks at the core Tcf3 promoter region which are likely to regulate Tcf3 expression. Notably, these modifications occur shortly after Wnt stimulation and it is plausible to think that the chromatin modifications within the Tcf3 locus can trigger the downregulation process of Tcf3 expression which can be stabilized further on via miR-211 function. Epigenetic regulation through histone modification or DNA methylation was also shown previously for other antagonists of Wnt signaling such as DACT3, sFRPs, WIF1 and DKK-1 in different cancer cells [48,49,50,51]. Further experiments are required to clarify whether this mode of gene repression is a general mechanism for Wnt-induced gene silencing in embryonic stem cells and tumor cells. Although the mediator of the observed chromatin modifications downstream of Wnt signaling remains elusive, we found that the putative cis-acting element, if any, is not located in the 6.7 kb promoter region which was previously described to regulate Tcf3 expression in different cell types [31]. Further work is needed to identify and study these cis-acting elements which might be of potential interest for providing further insight into the transcriptional repression downstream of Wnt signaling.
As an additional regulatory mechanism, we also described a novel Wnt-induced micro RNA, miR-211, and demonstrated that it targets Tcf3 in ApcNN ESCs. However, miR-211 overexpression in wild type ESCs does not reduce Tcf3 levels to the same degree as observed in ApcNN ES cells thus suggesting that multiple Wnt-mediated mechanisms are likely to exist. On the other hand, microRNAs usually exert their function by targeting multiple genes and it is plausible that miR-211 inhibits early neural differentiation in mESCs by repressing target genes other than Tcf3. Further experiments are required to characterize the loss of miR-211 function phenotype in mouse ESCs in order to evaluate the long-term effects on neural differentiation. The co-transfected with the Tcf3-39-UTR luciferase vector, and either with miR-211 or a non-targeting miRNA. Luciferase activity was measured 24 h posttransfection and normalized to Renilla luciferase signal. The same experiment was repeated with the mutant luciferase vectors, MTR1 and MTR2. Asterix represent P-value,0.01 and bars represent n = 36SEM. G. Flow cytometric analysis of Tuj1 and Nestin in miR-211 over expressing ESCs (miR-211 OE) and their controls (Emp) after 13 days of neural differentiation. Two independent clones were used for each genotype and representative example of each genotype is shown. Numbers in the graph represent the percent of cells in neural (green), progenitor (red) or negative (blue) populations. H. Histogram showing the relative expression of early neural markers Fgf5, Nestin, Pax6 and Sfrp2 in embryoid bodies derived from independent wild type ESCs clones stably expressing miR-211 or the corresponding empty vector. RNAs were isolated at different time points and were analyzed by qRT-PCR for different lineage markers. Bars represent n = 26SD. I. qRT-PCR analysis of Fgf5, Nestin, Pax6 and Sfrp2 in wild type ESCs stably expressing miR-211 or the corresponding empty vector, cultured for 24 h in N2B27 medium. Bars represent n = 26SD. doi:10.1371/journal.pgen.1003424.g007 observation that Wnt signaling induces miR-211 expression might also be of interest for other disciplines of research and in particular cancer. In line with our observation, a tumor promoting function has recently been described for miR-211 in colorectal cancer cells [52]. Accordingly, miR-211 has also been shown to play a key role in melanoma tumor formation and metastasis, as well as mesenchymal to epithelial transition (MET) [53,54,55] Taken together, we have revealed two downstream effects of Wnt signaling which contribute to the differentiation defects observed upon constitutive activation of canonical Wnt signaling, namely downregulation of Tcf3 expression and induction of miR-211. These cooperatively contribute to the inhibition of neural differentiation previously observed in Apc-mutant mouse ESCs [1]. We suggest that Wnt signaling represses Tcf3 expression possibly by altering the histone marks at the Tcf3 promoter and by activating miR-211 expression, thus extending our understanding of Tcf3 regulation in stem cells. In the future, additional studies are required to elucidate how these mechanisms contribute to the regulation of Tcf3 expression and, more in general, how Wnt signaling regulates stemness in embryonic and adult stem cells.

Ethics statement
This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was approved by the Committee on the Ethics of Animal Experiments of the Erasmus Medical Center (DEC permit numbers EMC 2351). All efforts were made to minimize suffering.

ES cell cultures and expression vectors
Apc 1638N/+ and Apc 1638T/+ animals, kept on an inbred C57Bl6/J background, were bred to derive ES cells from pre-implantation blastocyts according to previously described protocols [56]. Cells were cultured on MEFs inactivated by Mitomycin-C (Sigma) in Dulbecco's Modified Eagle's Medium (DMEM, Gibco) supplemented with 10% fetal calf serum (FCS, Gibco), L-glutamine (2 nM, Gibco), Na-Pyruvate (1 mM, Gibco), non essential amino acids (0.1 mM each, Gibco), 2-mercaptoethanol (55 mM, Gibco) and LIF (1000 U/ml, Milipore). Bruce 4 ESCs were purchased from American Type Culture Collection (ATCC) and Tcf3 2/2 and their wild type control GS1 ESCs were obtained as previously decribed [14]. To stimulate Wnt signaling in wild type ESCs, cells were cultured on gelatin coated dishes and treated with Wnt3aconditioned medium (collected from L-cells expressing Wnt3a plasmid) or L-control medium (collected from parental L-cells). Conditioned media were diluted 1:1 with ES medium and added to wild type ESCs for different time points. The Gsk-inhibitor SB-216763 was purchased from Sigma, dissolved in DMSO and used at 10 mM final concentration. DMSO was used as control in all the experiments.
Stable clones over expressing mmu-miR-211 were generated by transfecting Bruce4 wild type ESCs with miR-211 expressing plasmid pEZX-MR01 (Genecopoeia), or the corresponding empty vector. Several G418 resistant clones (200 mg/ml) were selected and validated for miRNA expression. In order to generate Tcf3 over expressing ESCs, ApcNN ESCs were co-transfected with pCAG-HA-Tcf3-IRES-EGFP (gift of Dr. Bing Lim, National University of Singapore, Singapore,) and Hygromycin resistance plasmid. Transfected ES cells were selected for Hygromycin (150 mg/ml). GFP expression in resistant clones was employed for validation purposes. Several independent clones were isolated and, upon validation by qPCR and western blot analysis for Tcf3 expression, employed for subsequent experiments.
The Tcf3-39-UTR plasmid was obtained by PCR amplification from mouse genomic DNA of a 565 bp fragment encompassing the Tcf3-39-UTR inclusive of the miR-211 target site (forward primer 59-AAATTGAGCTCTCCCCTTGCGCTGTGGTG-39; reverse primer 59-AAAAACTCGAGGGTGGGGGAAGGGG-CAGA-39). PCR products were digested with SacI and XhoI and ligated into SacI and XhoI-cut pmirGlo plasmid (Promega). All constructs were sequenced to verify their authenticity.

Microarray analysis
RNA was isolated using the RNeasy Mini Kit (QIAGEN) from cells lysed directly on the plate; a DNase step on the column was performed according to manufacturer's instructions. RNA quality was controlled by RNA 6000 Nano LabChip kit (Agilent Technologies). RNA was labeled using the GeneChip One-Cycle Target Labeling kit, hybridized to MOE430 2.0 arrays (Affymetrix) according to manufacturer's instructions. For data analysis, CEL files were uploaded and normalized using MAS 5.0 algorithm in Expression Console software (Affymetrix, Inc). Expression analysis was performed using Partek Genomics Suite 6.5 ((Partek Inc., St. Louis, MO) and Excel 2010 (Microsoft). A robust empirical method coupled with a validation step using qRT-PCR was used to confirm the modulation of gene expressions between different genotypes. A modulation of gene expression was validated when the observed fold-change is $1.5 and corresponding to none overlapping individual values, not present in the background. The unsupervised hierarchical clustering was performed after MAS 5.0 normalization, using Pearson's dissimilarity as distance measure and Ward's method for linkage analysis.

DNA methylation analysis
ESCs were cultured on 0.1% gelatin-coated dishes without MEFs for 2 passages and genomic DNA was isolated using DNeasy Blood & Tissue Kit (Qiagen). 1 mg of genomic DNA was used in bisulfite conversion reaction using EZ DNA Methylation Kit (Zymo Research) according to the manufacturer's instructions. Converted DNA was amplified by PCR using specific primers (Table S2) designed with Methyl Primer Express Software 1.0 (Applied Biosystems) or MethPrimer software [57].
The PCR amplification was carried out using KAPA2G Robust HotStart Taq DNA polymerase (Kapa biosystems) and PCR conditions were: 95uC for 3 min and 39 cycles of 95uC for 15 s, 57 or 53uC for 15 sec and 72uC for 15 sec, followed by 10 min at 72uC. PCR products from Region A, B and D were employed in direct sequencing using ABI BigDye Terminator and ABI 31306l genetic analyzer (Applied Biosystems).

Embryoid body formation
ESCs were trypsinized, re-suspended in ES medium and plated on gelatin-coated culture dishes for 30 min to remove MEFs. Nonattached ESCs were resuspended in EB medium (ESCs medium without LIF) at a cell density of 2610 5 cells/ml and plated on a non-adherent bacterial dish to initiate EB formation. EBs were collected by centrifugation (800 rpm for 5 min) every three days and re-suspended in fresh medium. 1/5 volume of EBs suspension was used for RNA extraction while the remaining EBs were kept in culture until day 12.

N2B27 short term differentiation
Differentiation assays were performed as previously described [42]. Shortly, cells were trypsinized and plated on gelatine-coated dishes in N2B27 medium consisting of DMEM/F12:Neurobasal medium (1:1, Gibco) supplemented with N2 and B27 (Gibco). Cells were harvested after 24 h or 48 h of differentiation for further analysis.

24/+4 neural differentiation assay
Neuronal differentiation of ESCs was induced as previously described [30]. Briefly, ESCs were trypsinized and incubated in ES medium on gelatine-coated dishes for 30 min. to allow attachment of MEFs. Non attached cells were collected and 3610 6 cells were cultured in 10 cm. non-adherent bacterial dishes (Greiner Bio-One) in EB medium for 8 days. Medium was refreshed every 2 days and 5 mM all-trans retinoic acid (Sigma) was added at day 4 and 6. On day 8 cells were trypsinized and plated on poly-L-ornithine/laminin-coated dishes at a density of 2610 5 cells/cm 2 in N2 medium. Poly-L-ornithine (Sigma) and laminin (Roche) were used at final concentrations of 0.1 mg/ml and 20 mg/ml, respectively.
After 48 h from cell plating, medium was changed to N2B27 and refreshed every 2 days. Cells were collected after 5 days of plating for further analysis.

Colony forming assay and alkaline phosphatase staining
Cells were trypsinized and plated on 0.1% gelatin-coated dishes for 30 min to remove MEFs. 500 FACS sorted cells were plated on each well of a gelatinized 24-well plate in N2B27 medium supplemented with different combinations of CHIR99021 (3 mM, Stemgent), PD0325901 (1 mM, Stemgent) and LIF (1000 U/ml, Milipore). Total number of colonies were counted after 5 days from plating upon staining with alkaline phosphatise (Milipore) according to manufacture's instructions.

Teratoma formation
Teratomas were obtained upon subcutaneous injection of 5610 6 cells (in PBS) into C57Bl6/J, for Apc-mutant ESCs (and their wild type controls), and NOD/SCID, for Tcf3 2/2 ESCs (and their wild type controls), recipient mice. Teratomas were collected after 2-3 weeks and used for further experiments.
RNA isolation, cDNA synthesis, and qRT-PCR RNA was isolated using Trizol (Invitrogen) or RNeasy Mini Kit (QIAGEN) and treated with DNase (Ambion) to remove contaminating genomic DNA. For gene expression analysis cDNA was synthesized using 1 mg RNA and the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo). microRNA expression analysis was performed using 40 ng of total RNA isolated by Trizol and employed in cDNA synthesis reaction using TaqMan MicroRNA Reverse Transcription kit (ABI). Realtime RT-PCR was performed using Applied Biosystems inventoried assays or TaqMan MicroRNA Assays on a 7900HT ABI real-time PCR system (Applied Biosystems). The Delta-Ct method was used to quantify the mRNA or miRNA relative gene expressions. Actb or snoRNA234 were used for normalization, respectively. qPCR analysis of the selected genes were performed using Fast SYBR Green Master Mix (ABI) and the primers listed in Table S2.

Immunofluorescence and confocal microscopy
Cells were harvested and fixed in 2% PFA for 20 min, washed with PBS, and permeabilized with 0.1% triton X-100 in PBS for 15 minutes. Cells were then incubated in Blocking solution (PBS, 4% FCS) for 30 min., stained overnight at 4uC with the primary antibody, washed and finally incubated with the secondary antibody for 2 h. Confocal analysis was performed with a Zeiss LSM510 confocal microscope. Tuj-1-Alexa488 was detected using a 488 nm laser and BP 500-550 emission filter. DRAQ5 was detected using a 633 nm laser and LP650 nm emission filter. Alexa 488-conjugated monoclonal anti-Tuj-1 was from Covance (A488-435L) and was used at 1:4000 dilution.

Flow cytometry analysis
Flow cytometric analysis was performed with a BD FACSAria III, using a yellow-green laser at 561 nm and a BP582/15 emission filter to detect anti-Nestin-PE antibodies, and 488 nm laser and LP502 and BP530/30 emission filters for Tuj-Alexa-488 antibodies. A Live-Dead-Fixable red staining (Invitrogen) was performed before fixation, to exclude dead cells and was detected using a 633 nm laser and BP660/20 emission filter.
Alexa 488-conjugated anti-Tuj-1 antibody was used at a 1:4000 dilution and the mouse anti-nestin antibody was from BD (556309) and was used at a 1:500 dilution together with a 2 nd Rat-antimouse PE-conjugated antibody (BD; 1:1000). DRAQ5 was from Biostatus and was used as recommended by the manufacturer.

Luciferase reporter assays
For the b-catenin/TCF reporter assay, 5610 5 ES cells were plated on 24-well plates seeded with MEFs and subsequently transfected by Fugene HD (Roche) with 250 ng of the TOP-Flash or FOP-Flash reporter constructs [28] together with 25 ng of the Renilla luciferase vector for normalization purposes. Luciferase activity was measured by Dual-Luciferase Reporter Assay System (Promega). Tcf3 promoter activity was evaluated in ApcNN and wild type ESCs similar to b-catenin/TCF reporter assay, as mentioned above and by using Tcf3-promoter constructs (kindly provided by Nina Solberg, SCI-CAST Innovation Center, Norway) and pGL3 empty vector a control. To examine the effect of Wnt3a treatment on Tcf3 promoter activity, cells were transfected with luciferase constructs and treated with Wnt3a condition medium or L-control medium for 48 h and luciferase activity was measured using Dual-Luciferase Reporter Assay System (Promega).
For the 39UTR-Luciferase reporter assay, HEK293 cells were plated in 24-well plates at a density of 0.5610 5 cells per well. Cells were co-transfected with 250 ng of UTR (or MTR) reporter plasmid and either mmu-miR-211 mimic or non-targetting oligos (40 nM, Dharmacon) using lipofectamin 2000 (Invitrogen).
Twenty-four hrs. after transfection, firefly-luciferase activity was measured by Dual-Luciferase Reporter Assay System (Promega) and normalized to the co-expressed Renilla luciferase signal.

Site-directed mutagenesis
Two mutant forms of the Tcf3-39UTR-luc plasmid were generated using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518). We introduced either 7 bp substitutions in the miRNA binding site (AAAGGGA into CCCTTTC) to generate the MTR1-Luc plasmid, or 4 bp (AAAGGGA into cAcGtGc) to generate the MTR2-Luc plasmid. The following mutagenesis primers were employed in the reaction: For MTR1, sense primer is 59-tctgaaatggtccccccccctgcatttccctttcctcaaggtgcctaccactgccttc-39 and antisense primer is 59-gaaggcagtggtaggcaccttgaggaaagggaaatgcagggggggggaccatttcaga-39. For MTR2 plasmid the sense primer is 59-gtccccccccctgcatttcacgtgcctcaaggtgcctacc-39 and the antisense primer is 59-ggtaggcaccttgaggcacgtgaaatgcagggggggggac-39.
The mutagenesis reaction was performed according to manufacture's instruction. Briefly, mutant strands were synthesized using the described primers followed by DpnI digestion of the amplification products to remove the parental methylated strands. Digestion reactions were transformed in XL10-Gold Ultracompetent cells and bacterial clones with correct nucleotide substitutions were used for further plasmid extraction.

Chromatin immunoprecipitation (ChIP)
ChIP was performed on wild type and ApcNN ESCs or on wild type ESCs treated for 12 h with Wnt3a conditioned medium or Lcontrol medium (1:1 dilluted with ES medium). Briefly, cells were fixed in 1% PFA for 30 minutes at room temperature and PFA was quenched afterwards with 125 mM glycine. Cells were washed with buffer B (0.25% Triton-X 100, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes, pH 7.6), buffer C (150 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes, pH 7.6). Cells were then sonicated in ChIP incubation buffer (0.3% SDS, 1% Triton-X 100, 0.15 M NaCl, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes, pH 7.6) using a BioRuptor sonicator (Cosmo Bio Co., Ltd) to obtain DNA fragments 200-700 base pairs. Chromatin was diluted in ChIP dilution buffer (with 0.15% SDS) and incubated with BSA-blocked protein-A/G Sepharose beads (Amersham) and 5 mg antibody overnight at 4uC. Antibodies used in this study include: H3K4me3 (Abcam, Ab8580-50), H3K27me3 (Upstate, 07-449), H3K9me3 (Abcam, Ab8898-100), H3Ac (Millipore #06-599) Beads were washed with buffer 1 (0.1% SDS, 0.1% deoxycholate, 1% Triton-X 100, 150 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes pH 7.6), buffer 2 (0.1% SDS, 0.1% deoxycholate, 1% Triton-X 100, 0.5 M NaCl, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes pH 7.6), buffer 3 (250 mM LiCl, 0.5% deoxycholate, 0.5% NP-40, 1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes, pH 7.6), and buffer 4 (1 mM EDTA, 0.5 mM EGTA, 20 mM Hepes, pH 7.6). Chromatin was eluted for 30 min at room temperature in elution buffer (1% SDS, 0.1 M NaHCO 3 ) and together with input chromatin, decrosslinked overnight at 65uC in the presence of 200 mM NaCl. DNA was extracted using QIAquick PCR Purification Kit and was used in QPCR analysis using Fast SYBR Green Master Mix (ABI) and primers indicated in Table S2. Figure S1 qRT-PCR validation of microarray results. Selected differentially expressed genes include Wnt and pluripotencyrelated genes. Measurements were performed in duplicates and using two independent cell lines per genotype. Actb was used for normalization. Plots represent average 6 SD of normalized qRT-PCR values for two independent clones of each genotype. (TIF)  Figure S3 Heat map showing the results of the qRT-PCR validation of microarray data relative to selected genes. Genes differentially expressed between ApcNN and wild type ESCs were compared to the list of genes differentially expressed between ApcNN and Tcf3OE cells (Table S3) Figure S5 A. Tcf3 promoter activity in Wnt high and Wnt low ESCs. Luciferase constructs containing different Tcf3 promoter fragments were co-transfected with Renilla luciferase and the relative promoter activity is shown after normalization to Renillaluciferase values. To monitor the effect of Wnt signaling on Tcf3 promoter activity, luciferase constructs were transfected in wild type ESCs, followed by 24 h treatment with Wnt3a-condition medium (Wnt3a) or L-control medium (LM). Similarly, luciferase constructs were transfected in 2 independent clones of ApcNN or wild type ESCs and promoter activity was measured after 48 h of transfection. The genomic location of different Tcf3 promoter fragments is depicted in the scheme. Bars represent n = 26SD. B. DNA methylation analysis of Tcf3 promoter. Schematic representation of the mouse Tcf3 promoter defined by the 59UTR and ,2 kb large CpG island extending into exons 1-3. For the purpose of methylation analysis, the CpG island was subdivided into regions A, B, C and D. Genomic DNA was first bisulfiteconverted and the individual regions either employed in bisulfitespecific PCR followed by DNA sequencing (region A, B and D), or used in methylation-specific PCR assays (region C). Arrows represent methylated (and un-methylated) specific primers for region C. PCR products from regions A, B and D were obtained from ApcNN, wild type and ApcN/+ (Apc 1638N/+ ) ESCs and directly sequenced. ApcN/+ ESCs were employed as controls since they express similar Tcf3 levels compared to wild type ESCs (data not shown). The sequencing results are depicted as open and solid circles for unmethylated and methylated CpG dinucleotides, respectively. Dashed circles represent CpGs which were not included in the PCR products. Because of its extremely GC-rich sequence, PCR amplification of region C was carried out using methylated-and unmethylated-specific primers (MSP and USP) covering 4 different CpG dinucleotides. Control DNA (mouse genomic DNA where all CpGs sites are enzymatically methylated by means of CpG Methylase) was used in all bisulfite reactions as a positive control. (TIF) Figure S6 Transient activation of Wnt signaling in wild type ESCs reduces H3Ac and H3K4me3 activating marks in Tcf3 promoter. Bruce 4 wild type ESCs were cultured on gelatin-coated dishes and treated with Wnt3a condition medium or L-control medium for 12 h. Cells were used for ChIP-QPCR as described before. Values from each amplicon were normalized to input chromatin. Since no amplification was detected at the negative region (P1) from some of the immunoprecipitated chromatin, values are shown as percent of input DNA. Bars represent n = 26SD. (TIF) Figure S7 A. Histogram showing relative expression of selected miR-211 predicted targets in ApcNN and wild type ESCs. Two independent clones were used for each genotype. Actb was used for normalization. Bars represent n = 26SD. B. Histogram showing relative expression of selected miR-211 predicted targets in wild type ESCs treated with Wnt3a condition medium or L-medium for different time intervals. The ratios of Wnt3a CM/L-medium are shown in the graphs. Bars represent n = 26SD. C. qRT-PCR analysis of miR-211 expression in wild type ESCs stably expressing miR-211 or the corresponding empty vector. Two independent ApcNN ESC clones were included for comparison. snoRNA-234 was used for normalization.Bars represent n = 26SD. D. Western blot analysis of the miR-211 predicted targets Sox11 and Sf3b1 in miR-211 over expressing cells and their wild type controls. (TIF)