{'fraction_non_alphanumeric': 0.04690026954177898, 'fraction_numerical': 0.044743935309973046, 'mean_word_length': 4.540298507462687, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Studies on enhancement of transverse rectus abdominis musculocutaneous flap survival by recombinant adenovirus mediated vascular endothelial growth factor 165 gene].

OBJECTIVE
To investigate the enhancement of the transverse rectus abdominis musculocutaneous (TRAM) flap survival in local ischemic area by recombinant adenovirus mediated vascular endothelial growth factor 165 gene (Ad-VEGF165).


METHODS
The vascular pedicle TRAM flaps were made in the right abdomen of 30 SD rats and they were randomly divided into 5 groups. The Ad-VEGF165 was injected into the subcutaneous tissue of epigastria(group 1), the subcutaneous tissue of epigastria and rectus abdominis muscle (group 2), and the rectus abdominis muscle (group 3); Adenovirus mediated green fluorescent protein (Ad-GFP) and DMEM were injected into the subcutaneous tissue of epigastria and rectus abdominis muscle as auto-control (group 4) and blank control (group 5), respectively. The survival areas of TRAM flap was measured after 7 days of operation. The microvascular density (MVD) and the integral optical density (IOD) were tested with anti-rat CD34 and with VEGF immunohistochemistry and in situ hybridization histochemistry (ISHH), respectively.


RESULTS
The survival areas of TRAM flap in groups 1, 2 and 3 (14.19 +/- 2.77, 15.18 +/- 2.18 and 8.30 +/- 1.28 cm2) were higher than those in groups 4 and 5(4.12 +/- 1.86 and 3.60 +/- 1.95 cm2), being significant differences (P < 0.05). The CD34 MVD of the TRAM flap in groups 1, 2 and 3 was higher than that in groups 4 and 5; the positive expression for VEGF and ISHH were shown in groups 1, 2 and 3 and there was significant difference when compared with groups 4 and 5 (P < 0.05).


CONCLUSION
Treatment by recombinant Ad-VEGF165 gene is an effective option for enhancement of the TRAM flap survival in the local ischemic area.