{'fraction_non_alphanumeric': 0.03288201160541586, 'fraction_numerical': 0.022243713733075435, 'mean_word_length': 5.017441860465116, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Studies on the Gram Reaction in association with Regeneration of Fungal Protoplasts

their morphology and Gram reactions during cultivation were examined. The procedures used were the thin--layer-agar plating method of Fukui et al. (1969) for the former and a simplified Gram stain for the latter. The protoplasts, Stmphylocoec"s a"rens and Escherichia celi were embedded together and treated with 6% glutaraldehyde containing O.75 M NaCl for 1-24 hr. Then, the material was treated succesively with O.2% crystal vielet, Lugol solution, dehydrating solution and safranin for 60, 6o, 60-9o ancl 20-30 sec, respectively. The extent of the Gram positive reaction in protoplasts was measured by comparison with that in the other representative bacteria embedded close to the protoplasts. Studies were also made on the Gram reactions of regenerating protoplasts with the following results. 1. Protoplasts were completely gram-negative before cultivation. 2. A gram-positive substance first appeared on the growing point of protoplasts, but