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Effect of Low-Level Laser and FM 1-43 on Prevention of Ototoxicity in Postnatal Organotypic Cultures of Rat Utricles

pyridinium dye, FM1-43, is a non-toxic, fluorescent, cationic dye, the fluorescence of which markedly increases after partitioning into membranes. In this study, the effects of LLL and FM1-43 on gentamicin-induced ototoxicity in postnatal organotypic cultures of rat utricles were investigated. Materials and Methods: Organotypic cultures of 4-day-old rat utricular maculae were established. In a series of experiments, utricles were exposed to either irradiation of a LLL (LG group), 10 μM FM1-43 (FG group), or both (LFG group), followed by 1 mM of gentamicin treatment for 12 hrs. The results of experimental groups were compared with the control group by confocal laser scanning and scanning electron microscopy. Results: LLL prevented vestibular hair cell ototoxicity. Rapid incubation with FM1-43 dye protected vestibular hair cell damage induced by gentamicin treatment. A substantial additive effect of LLL on the prevention of ototoxicity was noted in combination therapy with FM1-43. Conclusion: The results suggest that there is an additive protective effect of LLL and FM1-43 against gentamicin ototoxicity in postnatal organotypic cultures of rat utricles. LLL may have clinical preventive and therapeutic implications with respect to ototoxicity.