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Rapid and accurate direct injection chromatographic method for measuring volatile anesthetics in whole blood of dogs.

A modified rapid, accurate, and relatively simple gas chromatographic procedure was used for the analysis of a single hydrocarbon volatile anesthetic agent in whole arterial blood of dogs. Lengthy extraction procedures and laborious chromatographic separations were avoided. The glassware required was inexpensive and readily available, as were rubber and rubber-laminated Teflon septa which were excellent for making air-tight seals. Variations in blood pH, lipid content, protein concentration, hematocrit, and other components of whole blood will influence the solubility of the anesthetics. However, the procedure described in the present report was corrected for those factors by constructing standard curves with blood from each experimental dog. The arterial concentration of methoxyflurane (MTF) in 25 dogs was determined at 60 and 120 minutes of surgical anesthesia. Arterial concentrations of MTF at 60 minutes of anesthesia ranged from 7.29 to 41.7 mg/dl of blood, and at least 120 minutes, the range was from 9.02 to 55. There was no significant difference in arterial blood concentrations of MTF (P greater than 0.05) at 60 and 120 minutes of anesthesia.