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Expression of messenger RNAs of luteinizing hormone and follicle-stimulating hormone receptors in the granulosa layer during the ovulatory cycle of the hen

inhibin or activin in the presence of 5 ng/ml IGF-I (maximally effective dose being 10 ng/ml IGF-I). At the end of the culture period, the medium was collected. Monolayer cells were thereafter incubated with luteinizing hormone (10 ng/ml) for 4 h and medium was again collected. All media were stored at –20°C until assayed for progesterone by specific RIA. In a separate experiment, the effects of inhibin or activin on granulosa cell proliferation were determined over a 48 h culture period. Cells were lysed in DNA assay buffer (10 mM phosphate buffer, 2 M NaCl) containing trypsin/EDTA and total DNA was determined by the method of Labarca and Paigen (1980). The results showed that inhibin had no effect on either progesterone production or cell proliferation. However, activin at low doses up to 1 ng/ml tended to increase basal, LH and IGF-I-stimulated progesterone production but higher doses significantly inhibited progesterone production. Basal and IGF-I stimulated DNA synthesis was inhibited by activin in a dose-dependent manner. The results suggest that activin but not inhibin may be involved in the regulation of steroid production and follicular growth in the adult chicken ovary.