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Paradoxical findings in direct antiglobulin test and classification of agglutinating autoantibodies using eluates and monospecific anti‐human globulin sera

Agglutinating autoantibodies are rarely the cause of autoimmune haemolytic anaemia (AIHA) of warm type. These antibodies can be difficult to classify, and serological testing may result in confusion. Here, we describe the occurrence of paradoxical results in direct antiglobulin test (DAT) and a simple technique for the characterization of such antibodies.