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13C‐NMR detection of STD spectra

We have investigated the use of 13C for the detection of saturation transfer difference (STD) NMR spectra. By detecting the STD spectrum in the 13C channel it is possible to eliminate the residual water signal in the STD‐NMR spectrum. We have employed an INEPT transfer in order to shift the magnetization from the proton channel to 13C. As a sample system to check our method we have used human serum albumin and phenylalanine. We have shown that such a transfer can be accomplished and gives reasonable signal intensities. Copyright © 2009 John Wiley & Sons, Ltd.