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Indirect haemagglutination test using monoclonal antibody‐affinity purified antigens for diagnosis of human paragonimiasis due to Paragonimus heterotremus

An indirect haemagglutination test (IHA) using antigens purified by monoclonal antibody‐affinity chromatography was developed for the diagnosis of human paragonimiasis caused by Paragonimus heterotremus. Sera from patients with paragonimiasis (n= 30) were evaluated, along with sera from other parasitic infections (n= 92), pulmonary tuberculosis (n= 18) and healthy controls (n = 30). The sensitivity, specificity as well as positive and negative predictive value of the IHA, calculated at the prevalence of disease at 17.6%, were all 100%.