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A microfluidic device with automatic embryos trapping and co-culture with human stromal cells

In order to overcome the limitations of the traditional In Vitro Fertilization (IVF) culture method, this microfluidic chip is designed to integrate multiple functions to achieve the purpose of the embryos culturing in vitro. The chip combines the mechanism of dynamic flow resistance to trap and locate the embryos and co-culture with the stromal cells secreting the growth factors to give a better environment for embryos. Dynamic culture can not only carry metabolic waste off but also supply fresh medium to maintain stable osmotic pressure and pH value. The idea is combining the concept of flow-resistant by analogy with electric resistance to design automatic trapping structure. The advantages are reducing damage to embryo because of artificial operation and convenient observation. Finally, the research succeeded in giving birth to the mice.