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Amplification of ABCG2 Transporter Gene Exon 8 and 14 of Diabetic Patients

Diabetes is a serious illness affecting over 425 million people worldwide. Diabetes develops as a result of the failure of the pancreatic β-cells to produce the hormone insulin in the amount required to meet the body's needs. As a consequence of insulin deficiency, blood sugar levels rise and lead into macro vascular and micro vascular diseases of the kidneys, heart, eyes and nerves with passage of time. Elevated serum uric acid level is related with a variety of adverse health outcomes which includes gout, hypertension, diabetes mellitus, metabolic syndrome and cardiovascular diseases. Several genome-wide association studies on uric acid levels have implicated the ATP-binding cassette, subfamily G, member 2 (ABCG2) gene as being possibly causal. The aim of present study was to amplify exon 8 and exon 14 of ABCG2 gene of diabetic patients. Blood samples were collected from diabetic type II patients (n=25). Genomic DNA was extracted from blood using Phenol-Chloroform method, followed by amplification of Exon 8 and exon 14 was using Polymerase Chain Reaction (PCR). The size of amplified genes of DNA was analyzed by Gel Electrophoresis and then observed under gel documentation through UV rays.