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Internalization and trafficking assay for Drosophila larvae.

Over the last two decades, the Drosophila larval neuromuscular junction has gained immense popularity as a model system for the study of synaptic development, function, and plasticity. With this model, it is easy to visualize synapses and manipulate the system genetically with a high degree of temporal and spatial control, which makes it ideal for resolving problems in synaptic physiology and development. This protocol describes an assay that can be used for observing the cycling of transmembrane proteins at the plasma membrane and their trafficking within cells when there are antibodies available that bind cell-surface epitopes in vivo.