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Multiple components-pharmacokinetic studies on ten bioactive constituents of Peiyuan Tongnao Capsule, using parallel reaction monitoring mode.

Peiyuan Tongnao Capsule (PTC) plays an important role in clinical application due to its excellent curative efficacy in the treatment of ischemic stroke and chronic cerebral circulation insufficiency. To standardize and rationalize the clinical application of PTC, a rapid and sensitive method based on ultra-high performance liquid chromatography/quadrupole-Orbitrap mass spectrometry (UHPLC/Q-Orbitrap-MS) with parallel reaction monitoring mode (PRM) was developed and validated for the pharmacokinetic study. Ten bioactive compounds (aucubin, salidroside, echinacoside, paeoniflorin, verbascoside, liquiritin, 2, 3, 5, 4'-tetrahydroxy stilbene-2-O-β-D-glucoside, coumarin, glycyrrhizic acid, and emodin) were simultaneously determined in rat plasma. All calibration curves exhibited good linearity (r2 > 0.99). The lower limits of quantification were 0.082-13.291 ng·mL-1 . The intra- and inter-day precision were 0.54-12.36%, while the intra- and inter-day accuracy ranged from 100.45% to 114.00%. The mean extraction recoveries were 81.77-117.66% and the average matrix effects were 86.23-109.96%. The high extraction recoveries and acceptable matrix effects indicated that the pretreatment method was feasible. And the stability was acceptable under various storage conditions and processing procedures. The validated method was successfully applied to the multiple components-pharmacokinetic (PK) studies, which lay the foundation for further pharmacological and clinical research of PTC and may provide a reference for other TCMs.