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Determination of liothyronine and thyroxine in dried thyroid by GLC.

The analysis reported here was successful in the quantitative determination of liothyronine and thyroxine in driedthyroid. Three basic steps were employed: (a) hydrolysis of the dried thyroid to release the iodoamino acids from their protein linkage, (b) separation of liothyronine and thyroxine from the interfering substances by extraction and column chromatography, and (c) detection and quantitation by GLC.