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Analysis of the Fertility Restoring Gene Rf6 in T. timopheevii Cytoplasmic Male Sterility of Wheat with ISSR Markers

Inter simple sequence repeat (ISSR) was evaluated in a F 2 population of 147 plants derived from male fertility restorer line 2114 and male sterile line ND44A. Of 43 examined primers, 18 produced distinguishable as well as polymorphic bands between the two parents. Linkage analysis in the mapping population showed that two markers, UBC 808 and UBC 848, were closely linked with the restoring gene Rf6 of the T.timopheevii CMS system. The distance between the two markers and the restoring gene was 7.9 cM and 4.9 cM, respectively. Two parental plants were screened with 181 pairs of SSR primers, of which, 34.3% showed polymorphism. No locus was found linked with the restoring gene. Compared with SSR technique that used in the experiment, ISSR produced more information and proved to be a valuable method for identification of alien fragment.