{'fraction_non_alphanumeric': 0.03139968068121341, 'fraction_numerical': 0.031931878658861094, 'mean_word_length': 5.143790849673203, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Tissue concentration of transforming growth factor β1 and basic fibroblast growth factor in skin wounds created with a CO2 laser and scalpel: A comparative experimental study, using an animal model of skin resurfacing

Although a number of ablative‐laser techniques based on CO2 and Er: YAG laser devices have been successfully developed and used in the clinical setting, the bio‐molecular processes influencing wound healing after exposure to laser energy are not well elucidated. In this study, we aim to assess the impact of the mechanism of injury on the secretion of transforming growth factor β1 (TGF‐β1) and basic fibroblast growth factor (bFGF) in various stages of wound healing, in wounds created with a CO2 laser and scalpel. Ten Wistar rats were used to determine the levels of growth factor proteins TGF‐β1 and bFGF after CO2 laser‐ and scalpel‐induced skin injury. Tissue was excised on day 0 for untreated skin (control sites), and on days 1, 10, 30, and 90 following laser and scalpel surgery. Specimens were processed for histopathological analysis and for determining the concentration of growth factors by a Western blot technique. The concentration of TGF‐β1 increased markedly, at day 1 postinjury, from a baseline of 130±16 mm2 (mean surface area of blotted‐protein lanes) to 261±23 mm2 and 394±22 mm2 for laser‐inflicted injury and scalpel wounds, respectively; the latter values were found to differ significantly (p<0.001). The concentration of b‐FGF on day 10 postinjury differed significantly (p<0.001) between the laser sites (553±45 mm2) and the corresponding scalpel sites (418±41 mm2). Laser energy alters local tissue secretion of TGF‐β1 and bFGF of skin injuries created with the CO2 laser compared with wounds created with a scalpel. These differences might have an impact on various aspects of wound healing of skin injuries created by a laser.