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Free Amino Acid Profile and Expression of Genes Implicated in Protein Metabolism in Skeletal Muscle of Growing Pigs Fed Low-Protein Diets Supplemented with Branched-Chain Amino Acids.

Revealing the expression patterns of genes involved in protein metabolism as affected by diets would be useful for further clarifying the importance of the balance among the branched-chain amino acids (BCAAs), which include leucine (Leu), isoleucine (Ile), and valine (Val). Therefore, we used growing pigs to explore the effects of different dietary BCAA ratios on muscle protein metabolism. The Leu:Ile:Val ratio was 1:0.51:0.63 (20% crude protein, CP), 1:1:1 (17% CP), 1:0.75:0.75 (17% CP), 1:0.51:0.63 (17% CP), and 1:0.25:0.25 (17% CP), respectively. Results showed that compared with the control group, low-protein diets with the BCAA ratio ranging from 1:0.75:0.75 to 1:0.25:0.25 elevated muscle free amino acid (AA) concentrations and AA transporter expression, significantly activated the mammalian target of rapamycin complex 1 pathway, and decreased serum urea nitrogen content and the mRNA expression of genes related to muscle protein degradation (P < 0.05). In conclusion, these results indicated that maintaining the dietary Leu:Ile:Val ratio within 1:0.25:0.25-1:0.75:0.75 in low-protein diets (17% CP) would facilitate the absorption and utilization of free AA and result in improved protein metabolism and muscle growth.