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The first reaction intermediate complex of glutamate dehydrogenase from Corynebacterium gutamicum (584.5)

MSG (Monosodium glutamate) confers the umami taste. Corynebacterium glutamicum is an industrial bacterium used for glutamate production. Since glutamate dehydrogenase (CgGDH) produce glutamate via the reductive amination of α-ketoglutarate (α-KG), which is an intermediate of tricarboxylic acid (TCA) cycle, CgGDH is thought to be one of the key enzymes for glutamate production as well as glutamine synthetase and glutamine:2-oxoglutarate amidotransferase (GS/GOGAT) system in C. glutamicum. On the other hand, GDH is widely distributed among three domains of life and GDHs are also involved in nitrogen assimilation in those organisms. In spite of these importance of glutamate production and ammonia assimilation, the molecular mechanism of the reaction of GDH is not well elucidated. Here, we determined the crystal structure of CgGDH complexed with NADP+ and α-iminoglutarate (α-IG), an intermediate of GDH reaction, at 1.68 A resolution. CgGDH takes a homohexameric structure like GDHs from many other living orgam...