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Specular microscopy of corneal epithelium.

Specular microscopy was used to study the morphologic appearance of normal and regenerating rabbit corneal epithelium before and after corneal abrasion or heptanol application. Specular photomicrographs of the denuded area and healing epithelium were taken for two to three weeks as the epithelium regenerated, as well as prior to trauma. During the healing process the cells appeared much larger than normal and had an abnormal morphologic appearance. In addition, specular photomicrographs of the corneal epithelium in topically anesthetized human subjects were obtained. These results indicate that this method is suitable for diagnostic observation and clinical research in human subjects, as well animal experimentation.