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Chemical characterization and cytotoxicity of extracts of leaves of Virola sebifera of the cerrado tocantinense

Virola sebifera (Aubl.), belonging to the Myristicaceae family, popularly known as mucuíba, and ucuúba, is among the various species with medicinal use found in the Cerrado, has several ethnobotanical uses and medicinal benefits, being applied in folk medicine for the treatment of various illnesses. Based on this, the present study aimed to investigate the chemical composition of Virola sebifera using high performance liquid chromatography and analyze the cytotoxic activity of its leaf extracts. The leaf extracts were prepared using different methodologies: i) 70% ethanol in an ultrasonic bath, originating the crude ultrasonic extract (CEU); ii) 70% ethanol, in a soxhlet apparatus, originating the crude soxhlet extract (CES); and iii) sequential extraction in a soxhlet apparatus, starting with the hexane solvent, followed by methanol and 70% ethanol, originating, respectively, the hexane extract (HE), methanol extract (ME) and ethanol extract (EE). HPLC analyzes showed that extracts from V. sebifera leaves have a diverse matrix of phenolic compounds, including phenolic acids (syringic acid, p-coumaric acid, rosmarinic acid and chlorogenic acid) and flavonoids (morina, quercetin, catechin, hesperidin, naringin and rutin). The in vitro toxic activities were analyzed in CEU, CES, and EE and observed a great inhibition of Allium strain, this suggest an anticarcinogenic activity. The results obtained indicate that V. sebifera has potential antioxidant activity, due to the presence of a diversity of phenolic compounds, thus evidencing its medicinal potential, requiring further studies with the species in order to confirm the results obtained and direct its use safe.