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Regiospecific Internal Desaturation of Aliphatic Compounds by a Mutant Rhodococcus Strain

ABSTRACT A mutant Rhodococcus strain lacking the ability to utilize 1-chlorohexadecane was found to cis-desaturate aliphatic compounds, such as 1-chlorohexadecane,n-hexadecane, and heptadecanonitrile, yielding corresponding products with a double bond mainly at the ninth carbon from the terminal methyl groups. A new oxidative pathway involving thecis-desaturation step was suggested for alkane utilization by Rhodococcus spp.