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Screening for antioxidant properties of Salvia reflexa Hornem.

The antioxidant properties of the wild growing sage species, Salvia reflexa Hornem., were investigated. The presence of superoxide (O2·−) and hydroxyl (·OH) radicals, malonyldialdehyde (MDA), reduced glutathione (GSH) and total flavonoids were observed in the above‐ground parts of plant, as well as activities of the antioxidant enzymes superoxide‐dismutase (SOD) and peroxidase (P‐ase). The potential antioxidant activity of the methanol:water extract has been assessed based on scavenging activity of stable DPPH free radicals. By the means of TLC and LC/MS a screening for secondary plant products was performed. Significant quantities of O2·−, ·OH and MDA were observed. Thus, this species exhibited high SOD and P‐ase activities as well as a content of total flavonoids. The dominant naturally occurring compound was rosmarinic acid. The results obtained suggested a high antioxidant activity of Salvia reflexa Hornem. Copyright © 2000 John Wiley & Sons, Ltd.