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Multifunctional sol-gel sensing membrane for fiber optic glucose sensor

In this paper, Glucose oxidase (GOD) was immobilized on a novel silica membrane. The multifunctional sensing membrane was prepared by sol-gel method. GOD was immobilized on the aminated silica surfaces by glutaraldehyde cross-linking method. The fiber optical glucose sensor based on fluorescence quenching was designed and fabricated using lock-in amplifying technology to realize the detection of glucose concentration. The experimental results show that a linear range between phase delay φ and the glucose concentration of the solution was observed in the concentration range of 100 to 600 mg/dl and the detection limit is 50mg/dl, the sensor can meet the demand of clinical application. The response time of the sensing membrane was about 15s. The experimental results demonstrated that this biosensor with the multifunctional sensing membranes has high sensitivity, repeatability, good stability and fast response.