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An integrated microfluidic system for zebrafish larva organs injection

Zebrafish has been demonstrated to be an important model organism in the study of genetics, diseases and drugs. For investigating drug toxicity, we need to inject foreign substances into specific organs within zebrafish larvae. Traditionally, zebrafish larva microinjection is conducted manually and requires operators to control larva's orientation with flexible ends, which is time-consuming, labor-intensive, and inaccurate. In this paper, we present an integrated microfluidic system to facilitate zebrafish larva organs microinjection, which is capable of adjusting larvae to appropriate orientation conveniently. The head's direction of zebrafish is adjusted through a microfluidic chip and a series of pumps so that the larva moves tail-first at the exit of the microfluidic channel, where it is rotated around its body axis to a desired orientation. Finally, the binary image of the larva is analysed to locate the organs and injection is executed. Experimental results are presented to verify the efficiency of the proposed method.