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Research on Inhibiting Action of miRNA-144 with Carrier of Liposome Nanoparticle on Differentiation of Mesenchymal Stem Cells Osteogenesis

Liposome nanoparticles have inhibiting action on differentiation activity of mesenchymal stem cells (MSCs). This our study was focused on liposome nanoparticle transfection system (liop-NPs) and its inhibiting action on differentiation of MSCs osteogenesis. Cellular morphology of C3H/10T1/2
 was observed and content of ALP and BGP. The expressions of miRNA-144 and Cad-11 protein were detected. There was significant difference on cellular morphology in the liposome nanoparticle group compared with other two groups. The differentiated degree of osteogenesis was also low, and content
 of ALP in the liposome nanoparticles group was (6.67±0.57) ng/mL, which was lower than in the other three groups notably (P < 0.05). The content of BGP in the liposome nanoparticles group was (2.56±0.25) ng/mL, which was lower than in the other three groups (P
 < 0.05). The expression quantities of miRNA-144 in the liposome nanoparticles group at the 0, 7th and 14th days were (1.21±0.04), (1.63±0.06), and (2.04±0.03) respectively, which were higher than in the other three groups obviously (P < 0.05). The expression
 quantity of Cad-11 in the liposome nanoparticles group at the 0, 7th and 14th days were (0.87±0.03), (0.99±0.04), and (2.26±0.05) respectively, which was obviously higher than in the other three groups (P < 0.05). The structure of lipo-NPs was similar with that
 of phospholipid biomolecule. The lipo-NPs had the feature for nanoparticles, which had high efficiency and inoffensive transfection system with high specificity. The transfection efficiency of miRNA-144 was also increased, and its inhibiting action on differentiation of MSCs osteogenesis was
 enhanced.