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Semi-rational screening of the probiotics from the fecal flora of healthy adults against DSS-induced colitis mice by enhancing anti-inflammatory activity and modulating the gut microbiota.

Ulcerative colitis (UC), an chronic inflammatory bowel disease, substantially impacts patients' health-related quality of life. In this study, an efficient strategy for discovering high-efficient probiotics has been developed. Firstly, in order to survive in the conditions of the stomach and intestine, high bile salt-resistant and strong acid-resistant strains were screened out from the fecal flora of healthy adults. Secondly, the probiotic candidates were rescreened by examining the induction ability of IL-10 (anti-inflammatory factor) production in dextran sodium sulfate (DSS)- induced colitis mice, and Lactobacillus sakei 07 (L07) was identified and selected as the probiotic P. In the end, fourteen bifidobacterium strains isolated from healthy man stools were examined for their antimicrobial activity. Bifidobacterium bifidum B10 (73.75 % inhibition rate) has been selected as the probiotic B. Moreover, the colonic IL-6 and TNF-α expression of the DSS- induced colitis mice treated with L. sakei 07 (L07) - B. bifidum B10 combination (PB) significantly decreased and the IL-10 expression were up-regulated by PB compared to the DSS group. Furthermore, Bacteroidetes and Actinobacteria decreased and Firmicutes increased in DSS group mice, significantly. More interesting, intestinal flora biodiversity of DSS colitis mice was increased by PB. Of those, the level of B. bifidum increased significantly. The Bacteriodetes/Firmicutes (B/F) ratio has increased, the concentration of homocysteine and LPS in plasma has been down-regulated by PB in DSS-induced colitis mice. Upon administration of PB, the intestinal permeability of the DSS-induced colitis mice was decreased by approximately 2.01-fold. This method is expected to be used in high-throughput screening of the probiotics against colitis. In addition, the L. sakei 07 - B. bifidum B10 combination hold the potential in UC remitting by immunomodulatory and gut microbiota modulation.