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Influence of Lipid’s Main Transition Temperature on the Stability of Chimeric Liposomal Systems



The incorporation of polymeric components into liposomes
promotes structural rearrangement of the lipid bilayers that could affect their properties and
their behavior. Therefore, by mixing phospholipids with polymeric compounds the, socalled
chimeric liposomal nanosystems are produced and could be advantageous, compared
with conventional (e.g. composed of pure phospholipids) liposomal nanostructures.



In this work, we used lipids with different main transition temperature (Tm) i.e
1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, Tm=55°C), L-α-phosphatidylcholine,
hydrogenated (Soy) (HSPC, Tm=52 °C) and egg phosphatidylcholine (EggPC, Tm=23 °C)
and we studied and compared the physicochemical characteristics and the stability of
conventional with that of chimeric liposomes.



Thin-film hydration method (TFH) was used as the preparation protocol for all
systems. Dynamic and electrophoretic light scattering (DLS and ELS) were utilized in
order to elucidate the physicochemical characteristics of all systems. All liposomal systems
exhibited sizes below 100nm while the ζ-potential was around zero, indicating the
absence of surface charge.



 The results revealed that the Tm of each phospholipid influences the biophysical
behavior of the lipidic membrane, which contributes to the physicochemical characteristics
and affects the physical stability of the liposomal nanosystems. The nature and physicochemical
properties of each phospholipid seem to play a key role, regarding the structural
characteristics and the formation process of the liposomal nanosystems.



: Comparing the physicochemical properties of the conventional liposomes
with those of the chimeric liposomal systems, we conclude that the complexity of the latter,
due to the incorporation of the polymeric guest into the lipidic bilayer, revealed new
properties, which correspond to increased physical stability.
