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Feature extraction and classification of wide angle optical scattering patterns from single aerosol particles

The aim of this investigation is to discriminate between elastic light scattering patterns produced by reference materials, namely clusters of polystyrene (PSL) spheres, and by spores of Bacillus globigii (Bg), a known simulant of anthrax. TAOS (two-angle optical scattering) is an experimental technique which records the intensity patterns of laser light scattered by single aerosol particles over an extended range of the scattering angles θ and φ [1]. Particles are produced at a controlled rate and illuminated by a Q-switched Nd:YAG laser at λ = 532 nm. The TAOS patterns are recorded by an intensified CCD camera and stored for off-line processing. Typical patterns from PSL aggregates and BG spores are shown in Figures 1 and 2, respectively. Since in this context deterministic obstacle inversion is impossible, pattern recognition is a must. The pattern classifier under development consists of four stages. 1) Pre-processing. 2) Feature extraction by spectrum-enhancement [2]. 3) Training, in which principal component (PC ) analysis is applied to features extracted from a training-set (T ) of images and classification is rated