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A Corn (Zea mays L.) Bioassay Technique for Measuring Chlorsulfuron Levels in Three Saskatchewan Soils

Chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carboxyl]benzenesulfonamide} has recently been registered for the control of several broadleaf weeds in spring wheat (Triticum aestivum L.), durum wheat (Triticum durum Desf.), and barley (Hordeum vulgare L.) in western Canada. Residue carryover of this herbicide can cause injury to subsequent sensitive crops. A bioassay technique based on growth of pregerminated corn roots was used to detect levels of chlorsulfuron as low as 0.125 ppb in three Saskatchewan soils. Confidence levels of 95% were established. These confidence bands allowed the detection of chlorsulfuron levels within a minimum and maximum variation of 3.1 and 11.5% of root development.