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Zinc‐finger E‐box‐binding homeobox 1 (ZEB1) plays a crucial role in the maintenance of lung cancer stem cells resistant to gefitinib

Abstract Background Zinc‐finger E‐box‐binding homeobox 1 (ZEB1) is an important regulator of epithelial‐mesenchymal transition (EMT) and is involved in the maintenance of cancer stem cells (CSCs) via miR‐200c and BMI1 pathway. Recent studies revealed that ZEB1 contributes to the EMT‐mediated acquired resistance to gefitinib in EGFR‐mutant non‐small cell lung cancer (NSCLC). However, the precise role of ZEB1 in the maintenance of lung CSCs that lead to acquired resistance to gefitinib remains unclear. Methods PC9 and HCC827 NSCLC cell lines were treated with high concentrations of gefitinib, and surviving cells were referred to as “gefitinib‐resistant persisters” (GRPs). ZEB1 knockdown or overexpression was performed to determine the biological significance of ZEB1 in the CSC features of GRPs, and animal models were studied for in vivo validation. Expression of ZEB1, BMI1, and ALDH1A1 was analyzed by immunohistochemistry in tumor specimens from NSCLC patients with acquired resistance to gefitinib. Results GRPs had characteristic features of mesenchymal and CSC phenotypes with high expression of ZEB1 and BMI1, and decreased miR‐200c, in vitro and in vivo. ZEB1 silencing attenuated the suppression of miR‐200c, resulting in the reduction in BMI1 and reversed the mesenchymal and CSC features of GRPs. Furthermore, ZEB1 overexpression induced EMT and increased the levels of CD133‐ and BMI1‐positive GRPs in vitro and gefitinib resistance in vivo. Finally, ZEB1, BMI1, and ALDH1A1 were highly expressed in tumor specimens from EGFR‐mutant NSCLC patients with gefitinib resistance. Conclusions ZEB1 plays an important role in gefitinib‐resistant lung CSCs with EMT features via regulation of miR‐200c and BMI1.


INTRODUCTION
Progression-free survival (PFS) in patients with epidermal growth factor receptor (EGFR)-mutant non-small cell lung cancer (NSCLC) is limited due to acquired resistance to EGFR tyrosine kinase inhibitors (TKIs) such as gefitinib, 1 and investigations of the molecular mechanisms underlying resistance to EGFR-TKI are performed globally. The most well-known mechanisms of acquired resistance are EGFR T790M secondary mutation, MET amplification, overexpression of HGF, mutation or amplification of HER2, transformation to small cell lung cancer, and PIK3CA mutation. [2][3][4][5][6][7][8] However, 30% of the mechanisms of acquired resistance to EGFR-TKIs are not completely understood. 7 Cancer stem cells (CSCs), also known as tumorinitiating cells and stem-like cancer cells, express stem cell markers such as CD133, 9 BMI1, 10 ALDH1A1, 11 Oct4, 12 and CXCR4 13 in NSCLC. Several recent reports have suggested the involvement of lung CSCs in resistance to EGFR-TKI in NSCLC. 13,14 Accumulating evidence also suggests that the generation of CSCs is associated with epithelialmesenchymal transition (EMT), 15 which promotes malignant tumor progression. Moreover, EMT is also involved in resistance to EGFR-TKI. [16][17][18][19] Zinc-finger E-box-binding homeobox 1 (ZEB1) is a crucial EMT inducer in various human cancers. 20 Moreover, ZEB1 is also associated with stemness maintenance by repressing stemness-inhibiting microRNAs, including miR-200c. 21 A recent study reported that ZEB1 is involved in EMT-mediated acquired resistance to EGFR-TKIs. 22,23 However, the role of ZEB1 in the maintenance of lung CSCs that lead to acquired resistance to gefitinib is unclear.
In this study, using a short duration of treatment with high concentration of gefitinib, we established a unique model to obtain gefitinib-resistant persisters (GRPs) of EGFR-mutant NSCLC with stem cell features, in vitro as well as in vivo. We found that ZEB1 was highly expressed in the GRPs model and played important roles in the induction of EMT and maintenance of CSCs that eventually led to acquired resistance to gefitinib. We also integrated these in vitro and in vivo findings with clinical data from EGFRmutant NSCLC patients who experienced relapse after initial response to gefitinib.

METHODS
Cells, reagents, and establishment of ZEB1silenced and ZEB1-overexpressed lung cancer cell lines PC9 cells were kindly provided by Dr Kazuto Nishio (Department of Genome Biology, School of Medicine, Kindai University, Osaka). HCC827 cells were purchased from the American Type Culture Collection (Manassas). Cell lines were verified to be mycoplasma-free. Gefitinib was purchased from JS Research Chemicals Trading.
For a stable knockdown of ZEB1 (shZEB1), following sequences were annealed and cloned into the pLKO1-puro vector: shZEB1 forward, 5 0 -CCGGGCAACAATACAAG AGGTTAAACTCGAGTTTAACCTCTTGTATTGTTGCT-TTTTG-3 0 ; shZEB1 reverse 5 0 -AATTCAAAAAGCAAC AATACAAGAGGTTAAACTCGAGTTTAACCTCTTGTA-TTGTTGC-3 0 . The control plasmid was MISSION nontarget shRNA control vector (Cat. Number SHC002, Sigma-Aldrich). Constructs were transfected into the lentivirusproducing 293FT cells and lentivirus-containing supernatant was transduced into the indicated cell lines followed by selection using puromycin (Invitrogen). To avoid off-target effects, transient knockdown of ZEB1 was also performed in PC9 cells (see Figure S2). Lentiviral vector expressing ZEB1 was kindly provided by Dr Shyamala Maheswaran (Center for Cancer Research, Massachusetts General Hospital and Harvard Medical School, Boston, USA). A cDNA encoding human ZEB1, conjugated with flag protein sequences, was cloned into the pLenti4 vector. Lentivirus packaging 293FT cells were transfected with the pLenti4-flag-ZEB1 plasmid or pLenti4-V5DEST using the ViraPower Lentiviral Expression System (Invitrogen); and, the lentiviruses released into the culture supernatant were transduced into PC9 and HCC827 cells, followed by selection with zeocin (Invitrogen) to obtain stable PC9-ZEB1 and HCC827-ZEB1 cells. The ZEB1-silencing and ZEB1-overexpression plasmid transfections were performed using Lipofectamine LTX and PLUS reagents (Invitrogen).
Generation of GRPs of PC9 and HCC827, quantitative PCR, immunofluorescence, and sphere formation assays were performed as previously described. 24 Light microscopy, western blotting, list of employed antibodies, small interfering RNA transfection, and list of qPCR primers, are described in Supplementary Materials and Methods S1.
Xenograft studies in NOG mice NOD/Shi-scid/IL-2Rcnull (NOG) mice (7-week-old, female) were purchased from the Central Institute for Experimental Animals (Kanagawa, Japan). All mice were shipped to Juntendo University and handled under pathogen-free conditions. The mice were housed in a room under controlled temperature (25 C), humidity, and lighting (12 h light/dark cycle). To determine the in vivo tumor growth of GRPs, 1 × 10 2 PC9 and PC9-GRPs cells were mixed with Matrigel (BD Biosciences) and injected into both the flanks of NOG mice. Tumor formation was evaluated, and tumor size was measured until 44 days after injection. Tumor volume was calculated using the following formula: volume (mm 3 ) = 0.5 × length × width 2 . The same protocol was employed for the GRPs of PC9-shZEB1. The tumor samples were divided for mRNA analysis and paraffin-fixed for fluorescence immunohistochemistry (F-IHC). Further, for the in vivo ZEB1-induced resistance study, 1 ×

Patient sample collection
Tumor samples were obtained from patients with lung cancer who visited the Juntendo University Hospital. Patients gave their consent prior to enrollment in the study under Juntendo University Institutional Review Board (IRB)approved protocols. All experiments conformed to the principles outlined in the WMA Declaration of Helsinki.

Statistical analysis
Statistical analyses were performed using GraphPad Prism 6.0 (GraphPad Software). Values were compared using a two-tailed Student's t-test. Differences between the means were considered statistically significant at p < 0.05.

GRPs have characteristic gene expression profiles of mesenchymal and CSCs phenotype and high ZEB1 expression in NSCLC cell lines
The NSCLC cell lines PC9 and HCC827, which harbor an activating EGFR mutation, were exposed to high concentrations of gefitinib (1 μM for PC9 and 2 μM for HCC827). Nine days after exposure to gefitinib, a small fraction of viable cells survived and remained, whereas most of the cells died within a few days. These surviving PC9 and HCC827 cells were termed GRPs (PC9-GRPs and HCC827-GRPs, respectively). GRPs were extremely quiescent, and further isolated for gene expression analysis. We have previously demonstrated that short tandem repeat (STR) profiles of the parental cells and GRPs were identical by analysis of genomic DNA, indicating that GRPs did not arise from contaminating cells. 24 Furthermore, neither the EGFR T790M mutation nor MET gene amplification was observed in PC9or HCC827-GRPs. 24 To identify the mechanism underlying gefitinib resistance in the cell line model, we first investigated the expression of EMT-and CSC-related genes in parental cells and GRPs. The qPCR analysis indicated loss of epithelial marker E-cadherin, and increased expression of mesenchymal markers including fibronectin, vimentin, and N-cadherin in PC9-and HCC827-GRPs than in parental cells (Figure 1(a)). Furthermore, CSC genes such as CD133, ALDH1A1, Nanog, Oct4, and CXCR4 were highly expressed in PC9-GRPs and HCC827-GRPs than in parental cells (Figure 1(b)). These findings suggest that the small population of NSCLC cells that were highly enriched for EMT and CSC-related gene expression could survive and remain viable upon treatment with gefitinib.
ZEB1 is associated with EMT induction and maintenance of CSCs by repressing miR-200c, which can target BMI1, a known regulator of stemness. 10,21,25 As shown in Figure 1(c), ZEB1 was highly expressed in both PC9-and HCC827-GRPs than in parental cells. Interestingly, expression of miR-200c was downregulated and BMI1 was upregulated in both GRPs than in parental cells (Figure 1 (c)). These findings suggest a role for ZEB1 in the persistence of the mesenchymal and CSC genotypes that are resistant to gefitinib in EGFR mutation-positive NSCLC via the ZEB1-miR200c-BMI1 axis.

GRPs maintain mesenchymal and CSC genotypes and high ZEB1 expression in vivo
To investigate whether PC9-GRPs maintain the gene expression profile of ZEB1-miR200c-BMI1 axis as well as mesenchymal and CSC genotypes in vivo, we injected 100 cells of PC9-parent and PC9-GRPs into both the flanks of NOG mice. Tumor growth of PC9-GRPs was significantly faster than that of PC9-parent cells (Figure 2(a)). PC9-GRPs tumors showed lower expression of miR-200c, and higher expression of ZEB1, BMI1, and other CSC-related genes such as CD133, ALDH1A1 and mesenchymal marker vimentin than PC9-parent tumors (Figure 2(b)). We also examined protein expression of ZEB1 and BMI1 by IHC. Thyroid transcription factor 1 (TTF1) staining was also used to confirm presence of cancer cells in the tumor specimens. As shown in Figure 2(c), both ZEB1 and BMI1 were highly expressed in cancer cells with TTF1 staining in PC9-GRPs tumors than that in PC9-parent tumors. These findings indicated that PC9-GRPs maintained the gene expression profile of ZEB1-miR200c-BMI1 axis as well as mesenchymal and CSC genotypes in vivo.
Further, to confirm this expression profile in gefitinibresistant tumors in vivo, we transplanted PC9 cells into NOG mice ( Figure S1). When the tumor volume reached approximately 75 mm 3 , as measured with digital calipers, tumorbearing mice were treated with gefitinib (20 mg/kg) via intraperitoneal injection (five times/week). After 14 days of gefitinib treatment, the tumors remained, and these tumors were referred to as gefitinib-resistant tumors (GRTs). PC9-GRTs retained lower expression of miR-200c, and higher expression of ZEB1, BMI1, and CD133, ALDH1A1, Nanog, CXCR4 and vimentin than PC-9 parent tumors ( Figure S1). These in vivo findings of PC9-GRTs were consistent with those of PC9-GRPs transplanted in NOG mice.

Silencing ZEB1 reversed mesenchymal and CSC features in GRPs
To explore the role of ZEB1 in the maintenance of mesenchymal and CSC phenotypes in GRPs, we stably knocked down ZEB1 expression in PC9 and HCC827 cells using short-hairpin RNAs (shRNAs) that specifically target ZEB1. The efficiency of knockdown of ZEB1 expression was confirmed by qPCR analysis (Figure 3(a)). Low ZEB1-expressing cells, termed PC9and HCC827-shZEB1, were treated with gefitinib to obtain GRPs. As shown in Figure 3(b), the morphology of both PC9and HCC827-shZEB1 GRPs was more epithelial, while sh-Control GRPs exhibited spindle-shaped morphology reminiscent of the mesenchymal phenotype. Knockdown of ZEB1 led to upregulation of miR-200c and significant reduction of BMI1 in PC9-and HCC827-GRPs than in control cells (Figure 3(c)). Knockdown of ZEB1 also led to a decrease in the expression of vimentin and increase in E-cadherin, accompanied by loss of mesenchymal morphology, suggesting that ZEB1 depletion reversed EMT in GRPs than in control cells (Figure 3(d)). Furthermore, CSC-related gene signatures, such as CD133, ALDH1A1, Nanog, Oct4, CXCR4 and SOX2 were also reversed by ZEB1 knockdown in both PC9and HCC827-GRPs than in control cells (Figure 3(e)). Protein expression of ZEB1, CD133, and BMI1 was confirmed by western blot analysis (Figure 3(f )). The same result was obtained for transient ZEB1-silenced GRPs using a small-interfering RNA (siRNA) transfection system ( Figure S2). These findings indicate that ZEB1 is required for the maintenance of mesenchymal and CSC phenotypes in GRPs in vitro. in ZEB1-depleted GRPs-spheres (Figure 4(b)). Next, we injected 100 GRPs of PC9-shControl and PC9-shZEB1 into both the flanks of NOG mice. Tumor growth of GRPs was significantly suppressed by knockdown of ZEB1 expression (Figure 4(c)). Silencing ZEB1 also significantly repressed the expression of BMI1, CD133, ALDH1A1, and mesenchymal marker vimentin than control (Figure 4(d)). Immunohistochemical findings revealed that BMI1, a known target of ZEB1, was suppressed by knockdown of ZEB1 in PC9-GRPs tumors than in control (Figure 4(e)). These findings indicated that ZEB1 contributes to the self-renewal ability and in vivo tumorigenicity in GRPs.

Overexpression of ZEB1 induced EMT and gefitinib resistance and increased population of gefitinib-resistant CSCs in vitro
To examine whether ZEB1 could induce EMT and enhance CSC features in EGFR-mutant NSCLC, we transduced lentiviruses carrying the ZEB1 construct into PC9 and HCC827 cells.
Overexpression of ZEB1 mRNA and protein was confirmed by qPCR and western blot analysis, respectively ( Figure 5(a)). PC9 and HCC827 cells overexpressing ZEB1 (PC9-and HCC827-ZEB1) exhibited spindle-shaped morphology as compared to PC9-and HCC827-mock cells. Furthermore, overexpression of ZEB1 suppressed the expression of E-cadherin and increased expression of mesenchymal marker ( Figure S3), reminiscent of EMT than mock cells.
ZEB1-overexpressing cells also had higher number of spheres than mock cells ( Figure 5(b)). These findings indicated that overexpression of ZEB1 could induce EMT and enhance CSC features in EGFR-mutant NSCLC cells. Further, to investigate whether high ZEB1 expression could induce gefitinib resistance in vitro, PC9 and HCC827 cells overexpressing ZEB1 or mock were exposed to various concentrations of gefitinib to estimate IC 50 values against gefitinib. As shown in Figure 5(c), overexpression of ZEB1 increased the IC 50 of gefitinib in both PC9 and HCC827 cells than mock. Next, to explore the implications of high ZEB1 expression in the maintenance of GRPs with CSC features, cells overexpressing ZEB1 or mock were treated with high concentrations of gefitinib to obtain GRPs. Overexpression of ZEB1 significantly increased expression of BMI1-and CD133-positive GRPs that could survive and remain viable after gefitinib treatment ( Figure 5(d)). These findings indicate that ZEB1 is implicated in resistance to gefitinib and increases the population of gefitinib-resistant lung CSCs in vitro.

High ZEB1 expression induced resistance to gefitinib in vivo
To test the biological significance of high ZEB1 expression in conferring resistance to gefitinib in EGFR-mutant NSCLC cells in vivo, PC9-ZEB1 and PC9-mock cells were implanted subcutaneously in NOG mice. When the average tumor volume reached 75 mm 3 , mice were divided into groups to receive vehicle, or 10 or 20 mg/kg of gefitinib via intraperitoneal injection (six times/week). The tumor size was measured during 14 days of treatment, and tumor volume was calculated every two days. Antitumor effect was estimated for individual tumors as percentage of tumor growth inhibition. As shown in Figure 6(a), in the vehicle-treated group, tumor growth of PC9-ZEB1 was significantly faster than that of PC9-mock (p = 0.0028). In the gefitinib-treated group, tumor growth was suppressed with gefitinib treatment to a lesser extent in mice bearing PC9-ZEB1 tumors than those bearing PC9-mock (Figure 6(a)), suggesting that the antitumor effect of both concentrations of gefitinib was significantly reduced in PC9-ZEB1 mice than that in PC9-mock mice ( Figure 6(b)). These findings indicated a crucial role of ZEB1 in resistance to gefitinib in EGFR-mutant NSCLC cells in vivo.

ZEB1 is highly expressed in tumor specimens of NSCLC patients with acquired resistance to gefitinib
Finally, to confirm the in vitro and in vivo findings, we analyzed the expression of ZEB1, BMI1, and ALDH1A1 by immunohistochemistry in lung cancer specimens obtained from 20 NSCLC patients before treatment and after relapse during treatment with gefitinib. As shown in Table 1, in the pretreatment clinical samples, weak expression of ZEB1, BMI1, and ALDH1A1 was observed in 12, 8, and 8 samples, respectively. After acquiring resistance to gefitinib, 11 patients exhibited an EGFR T790M secondary mutation (Table 1), 17 cases exhibited moderate to strong expression of ZEB1 (Table 1, Figure 7), and 14 cases showed moderate to strong expression of BMI1 (Table 1, Figure 7). Finally, all samples with acquired resistance showed positive ALDH1A1 expression (Table 1, Figure 7). Taken together, these findings indicated that ZEB1, BMI1, and ALDH1A1 were highly expressed in tumor specimens from NSCLC patients with acquired resistance to gefitinib.

DISCUSSION
Recent studies suggest the involvement of EMT and CSCs in acquired resistance to EGFR-TKIs in EGFR-mutant NSCLC cells. [26][27][28] However, the molecular mechanism for regulation of not only EMT but also CSCs in conferring resistance to EGFR-TKIs remains unclear. ZEB1 is a crucial EMT inducer that directly suppresses the transcription of miR-200c. 20 Further, miR-200c modulates the expression of BMI1, a known regulator of stem cell self-renewal. 10,25 Interestingly, miR-200c can also target ZEB1, and this loop linking ZEB1 and miR-200c is important for mesenchymal or epithelial differentiation. 20,29 ZEB1 is involved in EMT-mediated acquired resistance to gefitinib in EGFR-mutant NSCLC cells, 22 although the biological significance of ZEB1 in the lung CSCs contributing to acquired resistance to gefitinib has not been fully elucidated.
In this study, we treated PC9 and HCC827 NSCLC cell lines that harbor activating EGFR mutations with gefitinib. After gefitinib treatment for nine days, a subpopulation of cells survived, referred to as gefitinib-resistant persisters (GRPs). These cells showed high expression of ZEB1 and BMI1 genes, and reduced expression of miR-200c (ZEB1-miR200c-BMI1 axis). Moreover, mesenchymal markers, such as vimentin, fibronectin, N-cadherin, and stem cell-related factors were upregulated, along with loss of the epithelial marker E-cadherin. In vivo, GRP tumor growth was faster and maintained the gene signature: ZEB1-miR200c-BMI1 axis; high expression of mesenchymal and stem cell-related factors; and reduced expression of epithelial marker. Gefitinib-resistant tumor (GRT), the remaining PC9 tumor in mice after treatment with gefitinib, also showed similar gene profiles. Furthermore, a stable knockdown of ZEB1 significantly attenuated the suppression of miR-200c, which led to reduced expression of BMI1 genes and protein. Silencing of ZEB1 also reduced expression of stem cell-related markers, including CD133, Oct4, SOX2, Nanog, CXCR4, ALDH1A1, and the mesenchymal marker vimentin, and restored E-cadherin expression. Selfrenewal of GRPs, as shown by sphere formation capacity, was also decreased in ZEB1-silenced GRPs, while in vivo tumorigenicity of GRPs was compromised. The overexpression of ZEB1 resulted in EMT and CSC phenotypes, and importantly, increased IC 50 values of gefitinib and CD133-and BMI1-positive GRPs in vitro. Overexpression of ZEB1 also induced resistance to gefitinib in vivo, as observed in the PC9 xenograft tumor model. Finally, the lung cancer specimens from patients with acquired resistance to EGFR-TKI showed increased expression of ZEB1, BMI1, and ALDH1A, than in specimens before treatment. Taken together, the findings suggest that GRPs are enriched with EMT and CSC phenotype, and that ZEB1 plays an important role in maintaining not only EMT but also CSC phenotype in GRPs via the miR-200c and BMI1 pathways.
F I G U R E 7 Specimens from lung cancer patients with acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) show increased expression of zinc-finger E-box-binding homeobox 1 (ZEB1), BMI1, and ALDH1A1 proteins. Representative images of fluorescence immunohistochemistry staining of tumor samples from lung cancer patients pretreatment and after recurrence. Double staining using antibodies specific to either EGFR-19del or EGFR-L858R is performed to confirm the tumor area in the specimens. Scale bars indicate 200 μm. del, deletion Shien et al. established clonal gefitinib-resistant NSCLC cells and demonstrated that few resistant cells with EMT signatures exhibited CSC properties. 30 Of those, clones of gefitinib-resistant HCC827 cells showed high expression of ZEB1 and silencing of miR-200c. 30 They also demonstrated that silencing of miR-200c was associated with increased expression of LIN28B, which regulates stem cell self-renewal and miR-200c activity. The LIN28B axis plays an important role in cells with acquired resistance to EGFR-TKIs that harbor EMT features. 31 A recent study by Weng et al. also established osimertinib-resistant H1975 cells with mesenchymal features, and demonstrated that these cells were capable of forming larger spheroids and showed high expression of ZEB1. 28 Although knockdown or overexpression of ZEB1 was not performed, these previous reports strongly support our hypothesis that ZEB1 plays a crucial role in EGFR-TKI-resistant lung CSCs with EMT features.
Cufi et al. demonstrated that EMT-driven resistance to erlotinib could be overcome by reversing the high-ZEB1/ low-miR200c signature in vivo. 32 Silibinin, a potent natural agent derived from dried fruits of milk thistle (Silybum marianum), 33 suppressed mesenchymal gene expression including ZEB1 and reversed suppression of miR-200c signature in erlotinib-refractory tumors; and, complete abrogation of tumor growth was observed after cotreatment with erlotinib and silibinin. Consistent with the findings of the preclinical study using silibinin, silencing of ZEB1 restored miR-200c expression in GRP tumor model in our study, along with reduced tumor incidence and slower tumor growth in vivo. These findings suggest that the combination of EGFR-TKI with a ZEB1-targeting approach would be effective in preventing EMT and CSC-mediated resistance to EGFR-TKI.
The expression of ZEB1 in cancer cells from NSCLC patients with acquired resistance to EGFR-TKI has been previously investigated. 22 However, to the best of our knowledge, no study has examined the expression of both ZEB1 and BMI1 in lung cancer specimens from patients with acquired resistance to EGFR-TKI. The current study revealed that ZEB1 protein expression was higher in cancer cells than in before-treatment cancer cells. This is consistent with the in vitro findings of our study using NSCLC cell lines, in which ZEB1 expression was significantly upregulated in the GRP of PC9 and HCC827 than in parental cells. Lung tumor specimens from NSCLC patients with acquired resistance to EGFR-TKI also showed high BMI1 protein expression, suggesting that the ZEB1-BMI1 axis exists in the clinical setting. Therefore, ZEB1 and BMI1 expression may be useful in monitoring resistance to EGFR-TKI in NSCLC patients who were treated with EGFR-TKI.
There are several limitations of this study. First, we did not use NSCLC cell lines with EGFR L858R mutation, although we showed that ZEB1 was highly expressed in tumor specimens from NSCLC patients with EGFR L858R mutation after gefitinib resistance. Second, osimertinib, a third-generation EGFR-TKI, is also currently used for the first-line treatment of EGFR-mutant NSCLC patients as the standard of care. To address the biological significance of ZEB1 in lung cancer stem cells resistant to osimertinib in this clinical setting, further studies to establish osimertinibresistant persisters from EGFR-mutant NSCLC cells are necessary. In addition, relapsed tumor tissues from EGFRmutant NSCLC patients after first-line treatment with osimertinib are required.
To the best of our knowledge, this is the first study to reveal the central role of ZEB1 in conferring resistance to gefitinib by not only inducing EMT but also by maintaining the CSC phenotype in NSCLC by regulating expression of miR-200c and BMI1, along with activating EGFR mutations. We propose a combination of EGFR-TKI with ZEB1-targeting therapy to prevent the survival of NSCLC cells that show EMT and CSC phenotypes. Further studies using ZEB1-targeted therapy, such as silibinin, would be required to verify the results of this study.