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Microfluidic device by using nano-magnetic beads conjugated antibodies for immunoassay

We developed a fast and precision immunoassay-based microfluidic assay system that consisted of a microfluidic analytic cartridge and immunoassay reagents for point-of-care testing (POCT). The nano-magnetic beads conjugated antibodies, HRP-labeled analog, and color reagent were all pre-loaded and packaged in a microfluidic analytic cartridge. The whole assay process was completed within 30 min without any additional washing or loading of reagents once the sample was injected in the microfluidic analytic cartridge. The performance of the immunoassay-based microfluidic assay system had been examined by using mouse IgG model. The results indicated that the dynamic range of the measurement could be approached to two orders with low deviation. The present study demonstrated that the performance of our immunoassay-based microfluidic assay system was suitable for POCT for healthcare professionals or patients.