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5‐Hydroxytryptamine, substance P, and thyrotropin‐releasing hormone in the adult cat spinal cord segment L7: Immunohistochemical and chemical studies

The terminal projections of the descending 5‐hydroxytryptamine (5‐HT) bulbospinal pathway and the coexistence among 5‐HT‐, substance P (SP)‐, and thyrotropin‐releasing hormone (TRH)‐like immunoreactivities (LI) in fibers innervating the L7 segement in the cat spinal cord were studied quantitively by use of the indirect double‐staining immunofluorescence technique. The content of 5‐HT, SP, and TRH in different parts of the spinal cord was determined by use of radioimmunoassay (RLA) (SP and TRH) and high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD) (5‐HT).