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Quantitative first‐pass perfusion MRI of the mouse myocardium

In this article, we present a first‐pass perfusion imaging protocol to determine quantitative regional perfusion values (in mL min−1 g−1) of the mouse myocardium. Perfusion was quantified using a Fermi‐constrained deconvolution of the myocardial tissue response with the arterial input function. A dual‐bolus approach was implemented. Experimental evidence is presented for the linearity of signal intensity in the left‐ventricular lumen during the prebolus (r = 0.99, P < 0.001) and in the myocardium during the full‐bolus injection (r = 0.99, P < 0.01) as function of Gd(DTPA)2− injection concentration used. The prebolus was used to reconstruct a nonsaturated arterial input function. Regional perfusion values proved repeatable in a cohort of nine healthy C57BL/6 mice. The perfusion values over two measurements with a 1‐week interval were 7.3 ± 0.9 and 7.2 ± 0.6 mL min−1 g−1, respectively. No effects of time (P > 0.05) and myocardial region (P > 0.05) were observed. The between‐session coefficient of variation was only 6%, whereas the inter‐animal coefficient of variation was 11 and 8% for the separate experiments. We expect that the first‐pass perfusion method here presented will be useful in preclinical studies of myocardial perfusion deficits and valuable to assess the impact of pro‐angiogenic therapy after myocardial infarction. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.