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Laminin A, B1, B2, S and M subunits in the postnatal rat liver development and after partial hepatectomy.

The expression of laminin subunits (A, B1, B2, S and M) in the perisinusoidal space of the rat liver was studied in early postnatal life, in the adult, and after partial hepatectomy. In the perisinusoidal space of the normal adult rat, laminin was detected with polyclonal antibodies only in small streaks of basement membranes extending from the portobiliary tract and to a lesser degree from the central vein. Occasionally, droplets of laminin immunoreactivity were also found along the intervening portions of the perisinusoidal spaces. All morphologically identifiable basement membranes of the rat liver (biliary ducts and blood vessels) irrespective of the age of animals exhibited B1, B2 and S immunoreactivity. Laminin A was restricted to the larger blood vessels and could not be detected in the biliary ducts. In the adult rat, immunoreactivity for the A-like M subunit was absent except for some negligible immunostaining of the blood vessels. In the neonatal rat strong linear laminin immunoreactivity was present in the perisinusoidal spaces throughout the entire lobule. Structurally, this laminin is not organized into a basement membrane as defined by electron microscopy. The perisinusoidal laminin was reactive with antibodies to B1, B2, S and M but the A subunit was absent. Sequential immunohistochemical studies of progressively older animals revealed that B1, B2, S and M immunoreactivity was weak in the perisinusoidal spaces before birth but during the postnatal life distinct linear immunoreactivities appeared. Most intense immunoreactivity was present at 1 to 2 weeks after birth. The perisinusoidal laminin gradually disappeared in growing animals and by 6 to 8 weeks basically no laminin could be detected in this location. By Northern blot analysis increased levels of B1 and B2 were detected in neonatal rats as compared to adult rats. S-laminin mRNA could readily be demonstrated in neonatal rat livers by Northern blot analysis, whereas A and M could not. Expression of S and M-laminin transcripts were demonstrated by polymerase chain reaction, but we were unable to obtain a laminin A product. After partial hepatectomy a transient laminin immunoreactivity, comparable to that in the neonatal rats, was detected in the perisinusoidal spaces. Laminin was most prominent 3 days after resection and reacted with antibodies to B1, B2, S and M. No A subunit could be detected in this extracellular matrix.(ABSTRACT TRUNCATED AT 400 WORDS)