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Analytical Molecular Diagnosis of Cervical Cancer via Paper Microfluidic Chip

In this work, we focused on the development of a novel point of care (POC) paper-based analytical microfluidic chip. The system has been developed for paper-based extraction, non-enzymatic amplification and electroanalytical detection of human papillomavirus (HPV). The device comprises paper extraction support material as well as paper-based detection tool. Herein, a special DNA modification method has been utilized to allow non-enzymatic amplification by using microbeads and silver nanoparticles labeled primers. The device is capable of extracting more than 10–100 copies per mL of DNA approximately in 15 min along with a single step extraction process from patient samples. In addition, it is able to detect low concentrations taking just less than 10 min with high selectivity to HPV kinds of 16&18. It only takes the low-cost point of care device less than 40 min with a low limit of detection (LOD) to complete the whole process.