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STUDIES ON ANTIMICROBIAL AND ANTIOXIDANT PROPERTIES OF OYSTER MUSHROOM PLEUROTUS FLORIDA

Centre of Advanced Study in Marine Biology, Faculty of Marine Sciences, Annamalai University 1 , Parangipettai608 502, Tamil Nadu, India PG and Research Department of Microbiology, Sengunthar Arts and Science College 2 , Tiruchengode637 205, Tamil Nadu, India ABSTRACT: The antimicrobial and antioxidant activity of four different solvent extracts of P. florida were investigated. The antimicrobial activities of different solvents were tested against different bacteria and fungi using disc diffusion method. Total antioxidant, reducing power and total phenolic content effect of the extracts was determined by phosphomolybdenum assay, 2,2-diphenyl-1picrylhydrazyl (DPPH) method and Folin-Ciocalteu method. Results indicated that the maximum antibacterial and antifungal activity was observed in ethanol extract of P. florida was found at 23 mm and 20mm against Streptococcus sp and E. floccosum respectively. Ethanolic extract of P. florida produced minimum inhibitory concentration (MIC) at 25mg/ml against E. coli followed by K. oxytoca (25mg/ ml), P. murabilis (75mg/ml) and Streptococcus sp. (50mg/ml). The ethanol extract exhibited good antioxidant activity (230 μg equivalent of BHT/g), strongest reducing power inhibition (79.24%) and also contain high phenolic content (6.25 mg gallic acid/g of dry extract). The results provided evidence that the ethanolic extracts of P. florida might indeed be potential sources of natural antioxidant and antimicrobial agents.