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Rapid Determination of Bile Acids in Bile from Various Mammals by Ultra-fast Liquid Chromatography

A valid and efficient ultra-fast liquid chromatography-diode array detector( UFLC-DAD) method was developed for the simultaneous determination of thirteen bile acids in the bile of three species,including rat,pig and gallstone patient. Chromatographic separation was performed with a Shim-pack XR-ODS column,and the mobile phase consisted of acetonitrile and potassium dihydrogenphosphate buffer( p H 2. 6) at the flow rate of 0. 5 m L / min. The linear detection range of most bile acids ranged from 2 ng / μL to 600 ng / μL with good correlation coefficient( r 0. 9995). All detected bile acids could be isolated with satisfactory resolution within 15 min,and the method was successfully applied in rapid screening of bile samples from three mammals.