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The effects of culture conditions on prolactin and growth hormone production by rat anterior pituitary cells.

The production of PRL and GH by rat pituitary cell cultures was evaluated over a 9-day period in 20 commercially available media supplemented with 5% horse serum (HS) or 5% calf serum. The quantity of PRL produced by cells maintained in a-modified Eagle medium (α-MEM) containing 5% HS was consistently greater than that in all other combinations of media and sera examined. PRL production in α-MEM was increased from 4.7 to 8.1 μg PRL/25,000 pituitary cells during a 9-day culture (72% ↑) as HS concentrations were increased from 1.25% to 20% however, the rate at which PRL was released into the medium during culture was unaffected by HS concentrations above 2.5%. Maximum hormone production was achieved when pituitary cells were cultured at 37 C in CO2-NaHCO3-buffered α-MEM at a pH of 7.8. Changes in medium osmolality from 250 to 350 mosm/kg H2O did not significantly affect PRL secretion. Under the conditions employed in this study, rat somatotrophs did not significantly increase their original GH content durin...