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[Relationship of nitric oxide with intrauterine human cytomegalovirus active infection during early pregnancy].

OBJECTIVE
To study the relationship of nitric oxide (NO) with intrauterine human cytomegalovirus (HCMV) active infection during early pregnancy.


METHODS
Reverse transcript polymerase chain reaction (RT-PCR) was used to screen HCMV-mRNA of chorionic villi during early pregnancy in 81 cases who had poor pregnant history. The serum HCMV-IgM was measured by enzyme linked immunoadsorbent assay (ELISA) and the expression of decidual iNOS-mRNA was investigated with in situ hybridization (ISH).


RESULTS
(1) Six cases were HCMV-mRNA positive in chorionic villous tissues and 14 cases with serum HCMV-IgM positive among cases, the positive rate of serum HCMV-IgM was 17.3% (14/81). All cases were divided into three groups according to HCMV-mRNA and serum HCMV IgM. Group I: 5 cases with HCMV-mRNA positive and IgM positive, and the transmission rate between pregnant women and fetus was 35.7% (5/14); Group II: 9 cases with HCMV-mRNA negative and IgM positive, Group III: 66 cases with HCMV-mRNA and IgM negative; In addition, 1 case with HCMV-mRNA positive but IgM negative. (2) The expressive quantity of decidual iNOS-mRNA in group I was significantly higher than those in group II and III (P < 0.05). The case with HCMV-mRNA positive and serum IgM negative also has a strong staining of decidual iNOS-mRNA.


CONCLUSIONS
NO may play a critical role in intrauterine HCMV active infection, NO could be served as an assistant marker of HCMV infection.