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Complete spectral assignments of cevadine and veratridine by 2D NMR techniques

The long‐range CH correlation NMR experiment (with decoupling of modulation of intensities due to one‐bond couplings during refocusing) and the double quantum coherence correlation spectroscopy (DQCOSY) experiment were used, together with the standard one‐bond CH correlation and COSY spectra, for complete 13C and 1H spectral peak assignments of veratrine (a 2:1 mixture of cevadine and veratridine). The long‐range CH correlation experiment determined the long‐range CH connectivity network, thus indirectly providing carbon–carbon connectivity. The DQCOSY experiment proved to be a complementary technique to the standard COSY experiment in unraveling and/or confirming proton–proton connectivities in this highly crowded proton spectrum.