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An in vivo study on comparison of disinfection of root canal with chemical disinfectants and disinfectant-diode laser-photodynamic treatment combined system

Background: Use of laser in dentistry is an approved modality of treatment in various specialties including endodontics. We aim to compare the disinfecting potential of the chemical method with laser alone and combination of laser photodynamic therapy with chemical method after rotary method of bio mechanical preparation. Materials and Methods: 38 patients were selected from the university clinics of Ajman University of science and technology Al Fujairah campus and divided them in to 3 groups who were clinically or /and radio graphically diagnosed cases of peri-apical disease. Percentage of bacterial growth reduction was assessed in both nonselective media like blood agar and in a selective media like mitis salivarius. In both media, percentage of bacterial reduction was analyzed with the ANOVA table. Results: The P-value (0.000) for Samples indicates that there is sufficient evidence that not all the means of samples are equal when a = 0.05 and in order to explore the differences among the means of the samples, it was found that the multiple comparison results were having similar means with regard to group A and group B. Moreover, it was noted that the mean of group C differs from the means of group A and B. Conclusions: In both of the microbiological study by Blood Agar and Mitis Salivarius media, it showed that application of either Diode laser or NaOCl alone will not bring considerable reduction in the bacterial colony. It was observed that the synergic effect of Diode laser combined with NaOCl was found to be very effective.