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Strain array for cell culture: cyclic strain affects epithelial cell spreading

We have designed, fabricated and characterized a strain array (6 mm well plate format) for cell culture that applies different strain to cells plated in each row of wells on the device. This device is capable of applying 0%, 2%, 4%, 7% or 17% strain to distinct samples with separate perfusion paths using a pressure difference of 10kPa. Our device has 25 wells but mimics the geometry of a standard 96‐well plate (for compatibility with automated plate readers and other technologies) and leaves samples easily accessible to assay. Further, samples experiencing different strain fields have separate perfusion pathways. These capabilities are useful in experiments to examine mechanotransduction mechanisms, specifically sensitivity to applied strain. Madin‐Darby Canine Kidney epithelial cells were attached on the device for 3 hrs then cyclically strained at 1Hz. Cells exhibited different patterns of spreading and localization of GFP‐tagged vinculin in response to strain levels over time.