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Cloning and expression of mannosylphospho dolichol synthase from capillary endothelial cell

The exact mechanism of switching to a new angiogenic phenotype during tumor growth is currently unknown. But, we have observed a direct relationship between the dolichol cycle and the cell cycle dynamics during in vitro angiogenesis. A non‐transformed capillary endothelial cell line when exposed to 2mM 8Br‐cAMP the cell proliferation enhanced ~70% with one‐half of the cell population in the S phase. A significant increase in the lipid‐linked oligosaccharide (LLO) biosynthesis and turnover, the expression of cell surface N‐glycans, and HSP‐70 and HSP‐90 was also observed. Mannosylphospho dolichol synthase (DPMS) catalyzes a ‘key step’ in the assembly of LLO. Its activity was upregulated during 8Br‐cAMP treatment. The objective of the present study is to address that DPMS undergoes a structural change following cAMP‐dependent protein phosphorylation. To test the hypothesis we have isolated a cDNA clone (bDPMS) after RT‐PCR screening of a cDNA library from bovine adrenal medullary capillary endothelial cells. DNA sequence indicated that bDPMS has 70% homology with the human DPMS, and alignment of the predicted amino acid sequence supported that bDPMS has a conserved sequence to be phosphorylated by cAMP‐dependent protein kinase (PKA). bDPMS has now been expressed as a 33 kDa His‐tag protein. We, therefore, conclude that PKA‐mediated phosphorylation motif on bDPMS is a potential angiogenic switch.