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Spatiotemporal quantification of actin dynamics by non-bleaching nanoscale imaging

Actin dynamics plays an important role in cell activities and drives a large range of cellular process such as cell division and cell motility. To visualize the actin dynamics, we introduce non-bleaching nanoscale imaging to capture actin dynamics over long time scales. Here, we demonstrate the high resolution quantification of actin dynamics by non-bleaching nanoscale imaging for the purpose of revealing resulting mechanical properties. Through this method, we visualized and quantified actin twisting dynamics through disassembly process by actin binding proteins.