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Spreading and segregation of lipids in air-stable lipid microarrays.

As a result of heterogeneous spreading of distinct lipids within the same microspots of air-stable lipid microarrays, ganglioside GM1 tends to segregate and thus enrich within the center area of microspots where being predetermined by the quill pin used for array fabrication, as indicated by the binding pattern of fluorescein-cholera toxin subunit B.