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Comparison of different 2D NMR techniques for 13C, 31P correlation

Three different experimental techniques are compared to find the most efficient protocol for 2D 13C, 31P correlation spectroscopy. It is shown that the best signal‐to‐noise ratio is given by the polarization transfer experiment for 31P detection and by the HMQC experiment for 13C detection. Applications of these techniques for 13C,31P correlation of β‐nicotinamide adenine dinucleotide (NAD+) and a mixture of three different mononucleotides are described.