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The Flp recombinase cleaves Holliday junctions in trans

The Flp site‐specific recombinase is encoded by the 2 µm plasmid of Saccharomyces cerevisiae and is a member of the integrase family of recombinases. Like all members of the integrase family studied, Flp mediates recombination in two steps. First, a pair of strand exchanges creates a Holliday‐like intermediate; second, this intermediate is resolved to recombinant products by a second pair of strand exchanges.