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Elevated levels of ERK2 in human breast carcinoma MCF‐7 cells transfected with protein kinase Cα

Abstract. We investigated the effect of elevated levels of protein kinase Cα (PKCα) on cell proliferation in human breast carcinoma cells (MCF‐7). MCF‐7 cells transfected with either the pSV2M(2)6 vector without the insert (MCF‐7/Vector) or containing a full length cDNA encoding PKCα (MCF‐7/PKCα) were compared. MCF‐7/PKCα cells were found to have an increased proliferative rate with a doubling time of 15 h as compared to 42 h for MCF‐7/Vector cells. Flow cytometry illustrated a greater percentage of MCF‐7/PKCα cells in the S phase of the cell cycle. Western and Northern blot analyses demonstrated an increase in extracellular regulated protein kinase 2 (ERK2) gene expression in MCF‐7/PKCα cells but no alteration of this gene expression in MCF‐7/Vector cells. These results suggested that the elevated level of ERK2 which is also known as mitogen activated protein kinase is probably involved in the increase in MCF‐7/PKCα cell proliferation.