{'fraction_non_alphanumeric': 0.020134228187919462, 'fraction_numerical': 0.01267710663683818, 'mean_word_length': 4.9118942731277535, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The modulatory effect of linoleic acid during Brucella abortus 544 infection in a murine macrophage RAW264.7 cell and a murine model BALB/c mice.

In this study, we investigated the effects of linoleic acid (LA) treatment on Brucella abortus infection in professional phagocytes RAW264.7 cells particularly during the pathogen's invasion and intracellular growth in these cells, as well as in a murine model BALB/c mice focusing on bacterial splenic proliferation and immunoregulatory activities. LA inhibited the growth of Brucella in a dose- and time-dependent manner. The ability of the pathogen to enter the phagocytes was inhibited as well as its survival within these cells. This is accompanied by increased nitrite accumulation in these cells at 24 h post-infection. The concentration of LA used in the present study did not affect the total body weight and the liver function of the mice. During Brucella infection, the total splenic weight of these animals was not changed but enhanced resistance to bacterial proliferation in the spleen. Furthermore, mice treated with LA displayed elevated levels of IL-12 and IFN-γ but reduced level of IL-10 during infection. The findings in this study showed the regulatory role of LA against B. abortus infection suggesting its potential use in designing intervention strategy for brucellosis.