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Comparative Proteomics Analysis of the Post‐Mitochondrial Fraction (S9 Fraction) of Human Lens‐Free Whole Eye and Liver

The increasing incidence of ocular diseases has accelerated research into therapeutic interventions for the eye. The knowledge of key metabolic mechanisms in the eye is vital for the successful ophthalmic drug discovery. The lens‐free whole‐eye post‐mitochondrial fraction(S9 fraction) is one of the most widely used in vitro system for ocular metabolism studies during early drug discovery stage. As of now, no report of a comprehensive proteomics investigation exists for the pooled ocular S9 fraction. The objective of this study was to explore the human ocular S9 proteome and compare it with the human liver S9 proteome.