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Large granular lymphocytes: Morphological studies

Large granular lymphocytes from normal human blood were enriched by centrifugation on discontinuous Percoll density gradients. Their capacity for natural killing, but not for phagocytosis of yeast cells, was demonstrated. Large granular lymphocytes are characterized in electron microscopy by their fine structure, especially by typical granules and by inclusions of tubular structures in a parallel array. Their lymphocyte nature is supported by activity of acid‐α‐naphthyl acetate esterase and by the absence of myelo‐peroxidase (POX) and of macrophage POX. The Fcγ receptor of their cell membrane is marked by soluble POX‐anti‐POX‐complexes; labeled parts of their membrane are not incorporated into the cytoplasm as in monocytes.