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Comparison of Two Real-Time PCR Methods for Diagnosis of Norovirus Infection in Outbreak and Community Settings

In a recent edition of the Journal of Clinical Microbiology, a real-time PCR test was described that used TaqMan technology to detect and differentiate between group 1 and group 2 noroviruses (NV) (2). We have previously described a SYBR green-based real-time PCR method based on primers Ni and E3, which has been our frontline test for outbreaks of NV since 2000 (1). We compared both assays on 217 stool samples sent to the West of Scotland Specialist Virology Centre from 6 nosocomial outbreaks plus 153 sporadic cases of acute gastroenteritis. All samples from outbreaks had previously tested negative for bacteria and parasites. However, microbiological data for the sporadic cases of gastroenteritis was absent.