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UNIT 2.6 Agonist-Stimulated [35S]GTPγS Binding

This unit presents a convenient method for studying receptor function using a binding assay to assess the initial step in receptor-mediated G protein activation in membranes prepared from rat brain. While the example procedure employs receptor agonists for serotonin1A receptors in the hippocampus, the method can be employed to study the activity of any substance that activates a receptor coupled to a G protein, particularly Gi. Alternate protocols describe modifications that can be used to simplify and increase the accuracy of the basic protocol, as well the use of the basic protocol to study receptor antagonists. 
 
 
Keywords: 
 
GTPγS; 
5-HT; 
serotonin; 
5-HT1A; 
hippocampus; 
membrane preparation; 
binding; 
G protein