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Amino acid uptake in rabbit blood vessels two weeks after induction of hypertension by coarctation of the abdominal aorta.

Rabbits were made hypertensive by partial constriction of the upper abdominal aorta. Two weeks after the operation various arteries and veins were removed from these rabbits and from sham-operated animals, and the in vitro incorporation of tritium-labelled proline and lysine into the vessels was measured to gain information on protein synthesis in the blood vessel walls. The ear and carotid arteries and the thoracic aorta, which are from the proximal (hypertensive) side of the ligature, showed increased uptake of the amino acids when compared with the same vessels from sham-operated animals. The increase showed good correlation with the degree of hypertension, expressed as the mean brachial arterial pressure. TCA-precipitated protein extracts were prepared from the thoracic and abdominal aortae. Comparison of labelled amino acid uptake into whole tissue and into TCA-precipitated protein for these vessels from each animal showed that increased uptake into the tissue indicated increased protein synthesis. Protein samples from thoracic aorta were separated into elastin, collagen, and other proteins for estimation of synthesis of these components. Each of the three protein groups showed increase of synthesis in hypertensive vessels. The greatest absolute increase was in the non-collagen, non-elastin protein, and the increase relative to control levels was similar in each of the three protein groups. In arteries distal to the ligature (normotensive side) and in veins from either side of the ligature no increase in protein synthesis related to brachial pressure was apparent.

These results show that the rate of protein synthesis, determined in vitro , increases in arteries subjected to increased blood pressure. Synthesis increases in both the static structural (collagen and elastin) and in the other proteins. The change in protein synthesis is caused by local, rather than systemic, factors.