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[Tissue tropism of novel nonenveloped DNA virus in experimentally infected rhesus monkey].

OBJECTIVE
To investigate the tissue tropism of transfusion transmitted virus (TTV), a novel nonenveloped DNA virus, in experimentally infected Rhesus monkey.


METHODS
Filtrate of feces of one patient with TTV infection was fed into the stomachs of ten Rhesus monkeys. Five (?) monkeys were killed during viremia and their tissues were collected to be examined for the presence of replicative virus DNA by in situ hybridization, dot blot hybridization with antisense probe and hybridization/nuclease protection assay.


RESULTS
The virus contents (density of electrophoretic band) in the liver, PBMC, and serum of three infected monkeys 50.1%, 31.0%, and 18.9% respectively; the relative proportion was 2.65;1.64;1.00. TTV was detected in liver, spleen, stomach, small intestine and its lymph nodes, PBMC, colon, and serum; however, viral plus-chain, a putative replicative intermediate, was found only in liver, spleen, small intestine, and lymphoid cells.


CONCLUSION
Transfusion transmitted virus is hepato-, spleno-, and lymphoid cell-tropic, and may has multiple routes of transmission.