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Osteogenesis and leukopoiesis within diffusion-chamber implants of isolated bone marrow subpopulations.

The developmental potential of isolated rabbit bone marrow cell populations was examined following autogeneous implantation into diffusion chambers. After 4 weeks, the implants were harvested and processed for light and electron microscopy. More total tissue was formed in abdominally implanted chambers than in corresponding femoral chambers. Two of the separated marrow cell populations produced significantly greater amounts of fibrous connective tissue, cartilage, and bone than did whole-marrow controls. These two populations, which were defined by density gradients of 1.050-1.055 gm/cm3 and 1.064-1.067 gm/cm3, consistently produced a fibrous connective tissue nodule in which were found dispersed foci of hyaline cartilage and woven bone. The denser population was distinguished further by the presence of leukopoietic foci in several of the implant chambers. Cartilage foci were found predominantly towards the center of the tissue nodules, whereas bone was dominant towards the periphery. Blood vessels, osteoclasts, bone remodeling, and mature lamellar bone were found only in those chambers which had been penetrated by the host's vascular system. The results indicate that 1) normal marrow tissue contains two separate osteoprogenitor cell populations; 2) these two progenitor populations represent separate osteogenic and chondrogenic capabilities; 3) one of these populations possesses a leukopoietic as well as an osteogenic potential; and 4) a competent vascular system is essential for the remodelling of bone into mature bone organs.