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[Hypocrellin B photosensitization effected a change of cytoplasmic free calcium concentration in ascitic hepatoma cells].

The Hypocrellin B (HB) is a new photosensitizer. HB-induced photodynamic changes of cytoplasmic free Ca2+ in ascitic hepatoma (HA) cells were observed using fluorescent calcium indicator Quin 2/AM. It was found that the increase in HB concentrations and exposure time followed by the increase in cytoplasmic free Ca2+. The singlet oxygen and hydroxyl radical generated by HB photosensitization were examined. The lipid peroxidation can be protected by Vit E; the relationship between the plasmic membrane depolarization and cytoplasmic free Ca2+ concentration by HB photodynamic action was investigated.