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Bound sugars in chick embryo fibroblasts.

Abstract Bound sugars in leukosis-sarcoma (L-S) virus susceptible and resistant fibroblasts were measured by the technique of isotope dilution after growth in media containing uniformly labeled [14 C]- d -glucose. After acid hydrolysis, the relative distribution of seven sugars in normal chick embryo fibroblasts was similar to that found in HeLa cells; fucose was the least abundant neutral hexose and glucosamine the most abundant amino sugar. Although small amounts of cells can be analyzed using this procedure, the composition and the dynamic state of membrane constituents is too complex for the detection of subtle differences in glycoprotein composition between normal phenotypes.