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Cardioprotective effect of bicyclol against ischemia‐reperfusion induced injury in anesthetized rats

The aim of the present study was to investigate the protective effect of bicyclol against myocardial injury induced by ischemia‐reperfusion (IR) and its underlying mechanism. Bicyclol was orally administrated to rats at the does of 50, 100 or 200 mg/kg/d for 3 days. Six hours after the last administration, myocardial injury was induced in anesthetized Sprague‐Dawley rats by coronary artery ligation for 1 h and then reperfusion for 3 h. The myocardial infarct size was measured by 2, 3, 5‐triphenyltetrazolium chloride staining. Homodynamic parametres, the levels of serum lactate dehydrogenase (LDH) and myocardial lipid peroxidation were also determined. Myocardial mitochondria membrane fluidity was measured by diphenylhexatriene. We found that pretreatment with bicyclol significantly reduced infarct size and LDH release induced by IR, and also improved left ventricular developed pressure compared with IR group. Futhermore, pretreatment with bicyclol markedly attenuated the decreases of myocardial mitochondria membrane fluidity and lipid peroxidation caused by IR. These results indicate that bicyclol has significant cardioprotective effect against ischemia and reperfusion.