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Potentially important variables identified by transvaginal ultrasound-guided embryo transfer.

Transvaginal ultrasound-guided embryo transfer was performed on 121 consecutive patients. Observation was made of guiding cannula and transfer catheter placement in relation to the endometrial surface and uterine fundus during embryo transfer. The position and movement of a transfer-associated air bubble and the impact of subendometrial myometrial contraction leading to endometrial movement was observed. Results indicate that tactile assessment of embryo transfer catheter placement is unreliable: in 17.4% of transfers the outer guiding catheter inadvertently abutted the fundal endometrium. The outer guiding cannula indented the endometrium in 24.8% and the transfer catheter embedded in the endometrium in 33.1%. Unavoidable sub-endometrial transfers occurred in 22.3% of transfers. Ultrasound-guided transfer avoided accidental tubal transfer in 7.4% of transfers. Transfer catheter withdrawal did not significantly affect embryo transfer-associated air bubble position. Endometrial movement due to sub-endometrial myometrial contraction was obvious in 36.4% of cases, with active motion of the transfer-associated air bubble occurring in 28.1%. Pregnancies occurred in 45.5% of transfers with endometrial movement compared to 15.6% (P < 0.001) without.