{'fraction_non_alphanumeric': 0.045513654096228866, 'fraction_numerical': 0.044027493962474455, 'mean_word_length': 4.7582887700534755, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 2, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 1, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Characterization of the complete chloroplast genome sequence of Chinese endemic species of Nouelia insignis (Hyalideae, Asteraceae) and its phylogenetic implications

Abstract This study was the first report complete chloroplast genome of Nouelia insignis (Asteraceae, Hyalideae), the large shrubs to small trees endemic to China. The circular whole cp genome of N. insignis was 151,524 bp in length, containing a large single-copy (LSC) region of 83,145 bp and a small single-copy (SSC) region of 18,261 bp. These two regions were separated by a pair of inverted repeat regions (IRa and IRb), each of them 25,060 bp in length. A total of 135 functional genes were encoded, consisting of 89 protein-coding genes, 38 tRNA genes, and eight rRNA genes. The overall GC content of the chloroplast genome sequence was 37.8%, and the GC contents of the LSC, SSC, and IR regions were 35.9, 31.5, and 43.2%, respectively. The phylogenetic analysis by the Bayesian analysis showed that the species of N. insignis was sister group with Gerbera jamesonii by strong support values, and thus was closely related to members of subfamilies of Cichorioideae and Pertyoideae. These results will be useful for the future studies of Asteraceae in the worldwide.

The species of Nouelia insignis Franch. (Asteraceae, Hyalideae) is a genus endemic to southwest China (Hind 2007;Gao and Hind 2011;Gong et al. 2011;Fu et al. 2016). They are narrowly and allopatrically distributed species, separated by the important biogeographic boundary Tanaka Line in Southwest China (Zhao and Gong 2015). Genetic knowledge of N. insignis would provide information for protection of this wild germplasm resource. Here, we obtained the complete plastome of N. insignis by Illumina sequencing technology (San Diego, CA). The complete plastome reported here will contribute to the further studies on the phylogenetic analysis of N. insignis.
Fresh leaves of N. insignis were collected from Wulaxi village (101 39 0 36 00 E, 28 37 0 12 00 N), Jiulong county, Sichuan Province, China. A specimen was deposited at the botany herbarium of Sichuan Normal University, SCNU (Associate Professor, Dr. Zhixi Fu, fuzx2017@sicnu.edu.cn) under the voucher number Z.X. Fu 2862. High quality total genomic DNA was extracted from ca. 6 cm 2 sections of the silica-dried leaf using improved Tiangen Plant Genomic DNA Kits, add the 4 ll RNAseA and 20 ll Proteinase K after incubated (65 C). Total DNA was directly constructed short-insert of 150 bp in length libraries and sequenced on the Illumina Genome Analyzer (Hiseq 2000) based on the manufacturer's protocol (Illumina, San Diego, CA) by ORI-GENE (Beijing, China). Generally, more than 5.2 Gb of data was obtained for complete cp genome of N. insignis; De novo assembly of CLC Genomic Workbench v11 (CLC Bio, Aarhus, Denmark) and consensus sequence of Geneious R11.1.5 (Biomatters Ltd., Auckland, New Zealand) with referenced chloroplast genome sequence of Gerbera jamesonii (accession no.: MN087227). The chloroplast genome was annotated using a web-based annotation program GeSeq (https://chlorobox.mpimp-golm. mpg.de/geseq.html) and editing by manual and imagining with OGDraw v1.2 (Lohse et al. 2013).
The complete chloroplast genome of N. insignis was 151,524 bp in length and a typical circular structure. The genome sequence data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi. nlm.nih.gov under the accession no. MT386594. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA694507, SRP303716, and SAMN17526016 (SRS8144786), respectively. It includes a pair of inverted repeat (IR) of 25,060 bp divided by a large single-copy (LSC) region of 83,145 bp and a small single-copy (SSC) region of 18,261 bp. The general G þ C content was 37.8% in the whole sequence and the corresponding values in the LSC, SSC, and IR regions are 35.9%, 31.5%, and 43.2%, respectively. The whole genome contained 135 genes, including 89 protein-coding genes, eight ribosomal RNA genes, and 38 tRNA genes, nevertheless, 114 unique genes, 20 genes duplicated in the IRs. In addition, among the annotated chloroplast genomic sequence, 15 genes possessed only single intron, two genes (ycf3 and clpP) possessed two introns.
To identify the phylogenetic position of N. insignis, we used a total of 30 additional complete cp genomes of the family Asteraceae and one outgroup taxa to clarify the phylogenetic position of N. insignis (Figure 1). All of the cp genome sequences were aligned in MAFFT (Katoh and Standley 2013). A maximum-likelihood analysis based on the GTRGAMMA model was performed with Bayesian method on the CIPRES (Miller et al. 2010;Ronquist et al. 2012) using 1000 bootstrap replicates. The phylogenetic analysis of the cp genome dataset recovers the similar clades as in previous phylogenetic work (Panero and Funk 2008;Panero et al. 2014;Fu et al. 2016). The Bayesian inference (BI) result with 100% bootstrap showed that N. insignis has a close sister relationship with the genus Gerbrea (Figure 1). The complete cp genome sequence of N. insignis will be the valuable resource for future studies on taxonomy and phylogeny of family Asteraceae and provides useful molecular data for further phylogenetic and evolutionary analysis.

Disclosure statement
No potential conflict of interest was reported by the author(s).