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T CELL RESPONSES TO ALLOANTIGENS II. IN VITRO INDUCTION OF SPECIFIC Ts BY HEAT‐INACTIVATED ALLOGENEIC LYMPHOID CELLS

When viable B6AF1 murine spleen cells were cultured in combination with heat-inactivated B10.D2 spleen cells under appropriate conditions, alloantigen-specific suppressor T cells (Ts) were generated. The optimal conditions for generation of specific Ts included a responder cell number of 2 to 4 106/ml, a high stimulator to responder ratio, the use of at least 30 min of heat treatment (45 C) of stimulator cells, and 6 or more days of culture.