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Radiobiological techniques applied to environmental problems.

The technology and methodology of specific cellular radiobiological techniques may have application in providing in vitro solutions to some current environmental toxicological problems. However, just as in the translation of radiobiological procedures to cancer chemotherapy studies, considerable care must be exercised. A variety of pitfalls are illustrated, including drug binding to growth surfaces, decay of drug activity, activity of agents released from inactivated cells, and the need for active intracellular metabolism for the correct estimation of some drug effects. These considerations apply, whether cytotoxicity or other end points such as mutagenicity or oncogenic transformation are measured. Two specific techniques described are the kinetics of recovery from potentially lethal damage as a possible, rapid method of assaying error-prone repair, and Arrhenius analysis as an aid in pinpointing specific mutagenic or carcinogenic reactions.