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Oral Fat Tolerance Test in Male and Female C57Bl/6 Mice

[Abstract] Postprandial hyperlipidemia is an important risk factor for cardiovascular diseases, and it can be evaluated by an oral fat tolerance test (OFTT). There is no consensus on how to perform the OFTT in rodents, especially in the C57BL/6 mice strain. Furthermore, there is no consensus in the literature regarding several variables involved in the test ( e.g. , dietary lipid source and fasting duration), and consequently, there is no standardization for the OFTT protocol. Therefore, we aimed to demonstrate a protocol for OFTT in male and female C57BL/6 mice. Briefly, mice underwent a 2 h-fasting period followed by blood collection from the tail and orogastric gavage of 200 μl soybea n oil. Blood was collected again at 1, 2, and 3 h after gavage, and centrifuged for triglyceride quantitation through a colorimetric enzymatic assay. Triglyceridemia was evaluated throughout the test, and the area under the curve was calculated. The present protocol contributes to standardizing the OFTT protocol in C57BL/6 mice and enables future research regarding the influence of sex and cardiometabolic diseases on postprandial lipemia.