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New Investigators Awards ‐ ANZSRS Oral Presentations

Background and objective:Measurement of ciliary beat frequency (CBF) and assessment of ciliary beat pattern are integral to the diagnosis of primary ciliary dyskinesia (PCD). Traditional clinical analysis measures CBF in real time, using photometry. We sought to compare and validate ciliary beat analysis using high-speed video microscopy with measurements made on the same samples using photometry. Methods: Samples of ciliated nasal epithelium were obtained from all patients referred to the Ciliary Diagnostic Group at Concord Hospital for Ciliary Function Testing using a standard brush biopsy technique. The epithelial stripswere examined at room temperature using an inverse-phase light microscope (IX70; Olympus, Tokyo, Japan). CBFwasmeasured initially using standard photometry (Maclab/4, Chart v3.2.6, Zeiss photometer). Ciliary motility of the same strips of epithelium was then recorded using high-speed video, at 120 fps (Basler acA6400-120um), and retrospectively measured using spectral analysis (Sisson-Ammons Video Analysis [SAVA] system). Comparisons between mean frequencies were made using paired t-tests and associations using Pearson’s correlation analysis. Results: Twenty-three patients (12 men : 11 women) with mean ± SD age of 9.3 ± 10.9 years were studied. Mean CBFmeasured by photometry (6.17 ± 2.69 Hz) was not significantly different to that measured by spectral analysis (6.18 ± 2.67 Hz) (P = 0.913). There was a strong correlation between values (R 2 = 0.9, P< 0.001). Conclusions: Ciliary beat frequency measured using spectral analysis is not significantly different to that measured using photometry. The SAVA system greatly reduces the time required to collect CBF data and gives greater control in selection of the region of interest. This reduces the necessity for real-time CBF analysis within a hospital clinic, alleviating the need to rely on a superseded, un-supported hardware and software system.