{'fraction_non_alphanumeric': 0.05993340732519423, 'fraction_numerical': 0.02774694783573807, 'mean_word_length': 4.1076487252124645, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Ba2+-induced changes in the Na+- and K+-permeability of the isolated frog skin.

Addition of the K+-channel blocking agent Ba2+ to the basolateral solution (in a concentration which is assumed to block the K+-flux via the K+-channels completely) resulted initially in a two-thirds reduction in the short-circuit current (SCC), followed by a complete recovery of the SCC. To examine the reason for this recovery, experiments were carried out which made it possible to calculate the Na+-permeability of the apical membrane (PaNa) and the K+-permeability of the basolateral membrane (PbK). The presence of Ba2+ had no significant effect on the cell volume and the cellular Na+- and K+-concentration. Addition of Ba2+ resulted in a depolarization of the intracellular potential (VSCC) from a control value of -76.3 +/- 2.8 mV to -15.1 +/- 1.7 mV. Although a complete recovery in the SCC was observed, VSCC did not recover. The K+-flux across the basolateral membrane was estimated from washout experiments. The washout of 42K+ (the K+-efflux) could be described by a single exponential component with a half time of 30-70 min. The addition of Ba2+ during the washout resulted in a transient decrease in 42K+-efflux from the epithelium. From VSCC and the cellular K+ and Na+-concentration and the coupling ratio of the Na-K pump, it was found that Na+-permeability of the apical membrane was 6.5 X 10(-7) cm X s-1 before the addition of Ba2+ and 1.7 X 10(-6) cm X s-1 when the SCC had recovered after the addition of Ba2+ and PbK changed from 8.8 X 10(-6) cm X s-1 to 1.5 X 10(-6) cm X s-1. Thus, the observed recovery in SCC was due to a considerable increase in Na+-permeability of the apical membrane and the presence or appearance of a small Ba2+-insensitive K+-permeability in the basolateral membrane.