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Bio-Modification of Carbonaceous Matters in Gold Ore: Model Experiments Using Powdered Activated Charcoal and Cell-Free Extracts of Phanerochaete chrysosporium

The detailed mechanism behind the bio-decomposition of carbonaceous matter in refractroy goold ore by Phanerocheate chrysosporium to facilitate improved cyanidation of gold is as yet undersdtood. To gain a better understanding of this mechanism, the present work model experiments using powdery activated carbon (PAC) and cell-free extracts of P. chrysosporium to simulate and focus on the biotransformation of carbonaceous matters in refractory gold ores. The results of solid characterization using SEM and XRD indicated a more non-uniform surface and smaller crystal sizes for PAC treaded with cell-free extracts for 72 hours. The significant decomposition of aromatic compounds into aliphatic compounds were observed in 13C-NMR and FTIR results for the high ratio of cell-free extract volume to mass of PAC. This results support the theory that one of the fundamental mechanisms behind the bio-decomposition process is aromatic bond cleavage by biomolecules produced by P. chrysosporium.