{'fraction_non_alphanumeric': 0.03531563934019596, 'fraction_numerical': 0.01525486791516805, 'mean_word_length': 5.0958230958230954, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 1, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Salivary Testosterone Levels and Health Status in Men and Women in the British General Population: Findings from the Third National Survey of Sexual Attitudes and Lifestyles (natsal-3)

Context: Salivary T (SalT) measurement by liquid chromatography–tandem mass spectroscopy resents the opportunity to examine health correlates of SalT in a large-scale population survey. Objective: This study sought to examine associations between SalT and health-related factors in men and women age 18 –74 years. Design and Setting: Morning saliva samples were obtained from participants in a cross-sectional probability-sample survey of the general British population (Natsal-3). Self-reported health and lifestyle questions were administered as part of a wider sexual health interview. Methods: SalT was measured using liquid chromatography–tandem mass spectroscopy. Linear regression was used to examine associations between health factors and mean SalT. Results: In men, mean SalT was associated with a range of health factors after age adjustment, and showed a strong independent negative association with body mass index (BMI) in multivariable analysis. Men reporting cardiovascular disease or currently taking medication for depression had lower age-adjusted SalT , although there was no association with cardiovascular disease after adjustment for BMI. The decline in SalT with increasing age remained after adjustment for health-related factors. In women, SalT declined with increasing age; however, there were no age-independent associations with health-related factors or specific heath conditions with the exception of higher SalT in smokers. Conclusions: SalT levels were associated, independently of age, with a range of self-reported health markers, particularly BMI, in men but not women. The findings support the view that there is an age-related decline in SalT in men and women, which cannot be explained by an increase in ill health. Our results demonstrate the potential of SalT as a convenient measure of tissue andro-gen exposure for population research. I n men, T deficiency with pituitary or testicular disease (male hypogonadism) is known to cause a wide range of symptoms and conditions including sexual dysfunction, loss of vigor, poor physical performance, fractures, and low mood, which can be effectively treated with testos-terone replacement therapy (TRT) (1). However, the more general relationship between male T levels and ill-health is less well understood. Community-based studies in men have shown associations between lower average serum testosterone (serum-T) and poorer health outcomes, including frailty, obesity, insulin resistance, cardiovascular diseases, and mortality, although findings have not always ISSN Print 0021-972X ISSN Online 1945-7197 Printed in USA This article has been published under the terms of the Creative Commons Attribution License (CC-BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author …

been consistent (2)(3)(4)(5). It is suggested that lower T levels in older men may contribute to these ageing-related conditions although questions remain about direction and causality (3). Some studies have also cast doubt as to whether there truly is an independent age-related decrease in T, or whether the observed decline is a biomarker of accumulated comorbidities (6). Currently, whether T treatment would benefit symptomatic older men with low T remains a subject of intense debate and research (7,8). Many of the existing studies have been carried out in middle-age or older men, and the health implications of lower T in younger men are unclear. In women, circulating concentrations of T are typically around 5-10% of those in men, with distinct age-related declines, independent of the menopause (9,10). The evidence for associations between T and health in women is even more equivocal than for men (10 -13). Furthermore, research efforts in women have been seriously hampered by inadequate sensitivity of serum-T measurements, due to the low concentration of T and poor specificity of commonly used immunoassay methods (14).
Salivary testosterone (Sal-T) is believed to represent tissue hormone levels, which are unaffected by variations in circulating binding proteins (15), thereby providing an alternative to serum free testosterone (free-T) in the assessment of androgen status. In contrast to the collection of serum, which is invasive and expensive, collection of saliva is relatively straightforward and requires minimal training. We have recently demonstrated that Sal-T can be reliably and accurately measured by a highly sensitive and specific liquid chromatography-tandem mass spectrometry method (16). In a validation study comparing samples from the same individuals, we found that Sal-T in adult men and women correlated more strongly with calculated serum free-T than serum total-T (17) and was also unaffected by variations in sex hormone-binding globulin (SHBG) (18). Fiers at al (19) confirmed the good correlation in both men and women between Sal-T and serum free-T measured by equilibrium dialysis but there was a significant systematic positive bias in women, which may reflect the influence of salivary protein binding on the lower female concentrations of Sal-T. The physiological and health-related behavioral correlates of Sal-T have not yet been explored.
Using data from the third National Survey of Sexual Attitudes and Lifestyles (Natsal-3), a probability-sample survey of British men and women, we investigated whether Sal-T is associated, independently of age, with demographic characteristics, lifestyle, general health, and reported health conditions. We hypothesized that relationships between Sal-T and health-related factors in men would be similar to those previously observed with serum free-T. To our knowledge, this is the first study to have examined the associations between Sal-T and health in a large community sample of men and women using a highly sensitive and specific assay, exploiting the theoretical and practical advantages of salivary measurements to the full.

Participants and procedures
Full details of the Natsal-3 methods, including details of the saliva sample collection and testing, are described elsewhere (20,21). Briefly, Natsal-3 was a probability-sample survey of 15 162 men and women age 16 -74 years resident in Britain. Interviews took place between September 2010 and August 2012 using a combination of computer-assisted personal interviewing and computer-assisted self-interview for the more-sensitive questions. The response rate was 57.7%. Single morning saliva samples were self collected from a subsample of men and women age 18 -74 years who did not regularly work night shifts. Consenting participants were given a self-collection pack and asked to provide their sample before 10:00 hours, to minimize diurnal variation in T (7). They were asked not to brush their teeth, eat, or chew before giving the sample, and to spit directly into a plain polystyrene tube. Saliva samples were posted to the laboratory where they were prepared and frozen at Ϫ80°C until analysis. On receipt of the sample, participants were sent a £5 voucher as a token of appreciation. Altogether, 9170 eligible participants were invited to provide a saliva sample: 6515 (71.0%) agreed to do so and 4591 samples were received by the laboratory and matched to the survey data (50.1% of those invited). A total of 463 samples were excluded due to sample quality issues (insufficient volume [n ϭ 154]; sample discolored/bloody [n ϭ 91]; sample recorded as taken after 10:30 hours [n ϭ 34]; period between sample being taken and received by the laboratory more than 5 days or unknown due to missing date of collection [n ϭ 172]; not tested due to error [n ϭ 12]) leaving 4128 participants (45.0% of those invited) with a T result (1675 men; 2453 women). This paper examines associations between Sal-T and health in the general population; therefore, 76 men and 330 women with clinical conditions or taking medication likely to affect T levels were excluded from analysis (individuals may be excluded for more than one reason): currently taking medication for epilepsy (15 men; 15 women) or prostate disease (43 men); treatment for ovarian, testicular, or pituitary condition (16 men; 23 women) or for polycystic ovaries (35 women) in the past year; pregnant at interview (42 women); current receipt of hormone replacement therapy (62 women); ever receipt of hormone replacement therapy together with having had a hysterectomy (proxy measure for having had ovaries removed; 181 women); missing data for these questions (three men; 15 women); resulting in 1599 men and 2123 women being included in the analysis. These exclusions aimed to minimize confounding of the relationship between T and health caused by these factors which are known to influence T levels, while retaining an otherwise-representative sample of the general population. Women taking hormonal contraception (HC) (oral contraceptive pill, mirena, injections, implants, or the contraceptive patch) were included in analyses to allow examination of the relationship between HC and sal-T, and to avoid biases which may result from excluding this substantial proportion of women (24% of all women with a valid saliva sample usually used a HC, and this was 67% in the youngest age group [age 18 -24 y]). However, additional sensitivity analyses were carried out excluding women taking HC, to assess whether their inclusion affected associations with health factors.

Measures
Health and medication questions were self reported, and body mass index (BMI) was calculated from self-reported height and weight. Due to small numbers of underweight individuals (BMI Ͻ 18.5 kg/m 2 ), these participants were excluded from analysis of BMI (14 men and 44 women). Most of the health questions were asked in the computer-assisted personal interviewing, with the exception of depressive symptoms (past 2 weeks) which were assessed in the computer-assisted self interview using a validated two-item patient health questionnaire (PHQ-2) (22). Cardiac, vascular disease, or hypertension were defined as ever being given such diagnoses by a doctor.
Season of data collection was included as a potential confounder, defined as: Winter (December, January, February), Spring (March, April, May), Summer (June, July, August), Autumn (September, October, November).
The liquid chromatography-tandem mass spectroscopy Sal-T assay was developed using strict validation criteria (23), with a lower limit of quantification of 6.5 pmol/L. Full details of the laboratory methods, including the validation of the assay, have been published elsewhere (17,18,20).

Statistical analyses
Statistical analyses were carried out using STATA (version 13.1) accounting for the complex survey design (stratification, clustering, and weighting of the sample) (24). We applied two weights: the survey weight corrected for unequal probability of selection and differential response (by age, sex, and region) to the survey itself; the additional saliva weight corrected for unequal probability of selection and differential response to the saliva sample. Factors found to be associated with providing a usable saliva sample included age at interview, ethnicity, self-reported general health, and sexual function; the saliva weighting reduced these biases (20).
Descriptive statistics are presented as means (SEs), with multivariable linear regression used to assess differences between groups. Throughout, we censored very high Sal-T values so that, for each 10-year age group stratified by sex, values above the 99th percentile were assigned a value equal to that of the 99th percentile. The Sal-T data for men were normally distributed; however, the distribution for women was positively skewed and so values were transformed on the natural log scale for analysis. Accordingly, for men we present linear regression coefficients representing differences in mean T in pmol/L, whereas for women we present ratios of geometric mean Sal-T obtained from exponentiated coefficients. Interval regression was used to assign values to the range 0 to 6.5 pmol/L for three men, and 0.5 (to allow log transformation) to 6.5 pmol/L for 62 women with T levels below the limit of detection (Ͻ6.5 pmol/L). Age was adjusted for using both linear and quadratic terms to account for a nonlinear relationship of T with age.
Given that several associations were found for men, multivariable analyses were used to determine which health factors were independently associated with Sal-T. Variables were grouped into a series of domains, to identify their individual contributions to the overall relationships with Sal-T after adjustment for earlier domains. The domains were: 1) age and season, 2) relationship status and children, 3) BMI, and 4) general health. A fifth lifestyle domain (smoking, alcohol consumption, drug use) contained no significant associations (either age adjusted or multivariable) and was therefore not presented in the final multivariable table. Within each domain the variables were entered into a forward stepwise model selection process (significance level for inclusion P Ͻ .1) with variables selected from earlier domains included with certainty. The ordering of domains began with factors for which the evidence of association was best established (from earlier publications) or of a demographic nature, and then proceeded to health-related factors. In this way, any identified associations between Sal-T and health could be seen as robust and not explained by confounding factors in other domains. An equivalent analysis was not performed for women given the lack of age-adjusted associations. In further analysis we examined associations between specific health conditions or medical treatments and Sal-T, adjusting firstly for age and then for those factors that had been cumulatively selected from domains 1 to 3.

Ethics
The Natsal-3 study was approved by the Oxfordshire Research Ethics Committee A (reference: 09/H0604/27). Written informed consent was obtained for anonymized testing of saliva samples, without return of results.

Age-adjusted associations of demographic and general health factors with mean Sal-T (Tables 1 and 2; Figures 1 and 2)
For both men and women, mean Sal-T decreased with increasing age, and seasonal variation was observed. Among men, there was variation in mean sal-T by relationship status after adjustment for age, with the highest levels among those who were not currently in a steady relationship, and lowest levels among those who were married or cohabiting (age-adjusted linear regression coefficient for those not in a steady relationship, compared with those married/cohabiting: 20.12 (95% confidence Abbreviations: unwt, unweighted; wt, weighted. a Those with BMI Ͻ 18.5 kg/m 2 have been excluded from analysis due insufficient numbers to analyze this group separately.
b Measure of comorbidity includes arthritis, heart attack, coronary heart disease, angina, other forms of heart disease, hypertension, stroke, diabetes, broken hip or pelvis bone or hip replacement ever, backache lasting longer than 3 months, any other muscle or bone disease lasting longer than 3 months, treatment for depression, treatment for cancer, and treatment for any thyroid condition in the past year. c Adjusted for age and age squared to account for nonlinear relationship between T and age. Abbreviations: unwt, unweighted; wt, weighted. a Those with BMI Ͻ 18.5 kg/m 2 have been excluded from analysis due insufficient numbers to analyze this group separately.
b Measure of comorbidity includes arthritis, heart attack, coronary heart disease, angina, other forms of heart disease, hypertension, stroke, diabetes, broken hip or pelvis bone or hip replacement ever, backache lasting longer than 3 mo, any other muscle or bone disease lasting longer than 3 mo, treatment for depression, treatment for cancer, and treatment for any thyroid condition in the past year. c Ratio of geometric means, obtained from exponentiated age-adjusted linear regression coefficients of log-transformed data for women. Adjusted for age and age-squared to account for nonlinear relationship between T and age.
doi: 10.1210/jc.2016-1669 press.endocrine.org/journal/jcem interval [CI], 6.41-33.82); no association was found with having children. There were no associations with demographic factors for women. Mean Sal-T was associated, independently of age, with a range of measures of general health in men but not in women. In age-stratified analyses of men, mean Sal-T was seen to be lower with increasing BMI (normal, overweight, obese) (Figure 1), and somewhat higher in those reporting good or very good health (Figure 2) at least at younger ages; these associations were not seen in women. Expressed as age-adjusted regression coefficients, we found levels were lower among men who were overweight or obese, ( The only health association found for women was with smoking, with higher Sal-T among women who smoked (ratio of geometric means 1.11 [1.02-1.22] for current smokers compared with nonsmokers). Women who used HC as their usual method of contraception had lower mean Sal-T (geometric mean ratio compared with those who had not taken HC: 0.78 [0.70 -0.87]; P Ͻ .0001). A sensitivity analysis excluding women on HC (n ϭ 499) produced generally similar results, although the association with smoking became nonsignificant due to an increase in the size of the confidence intervals (age-adjusted geometric mean ratio: 1.08 [0.98 -1.19]; P ϭ .107). Adjusting the women's analysis for season as well as age did not change any of the results (data not shown).

Multivariable analyses of men
In men, all associations with variables in domains 1-3 in the age-adjusted analyses remained significant in the multivariable analyses: age, season, relationship status, and BMI (Table 3). Only one variable from domain 4 -self-reported general health-was selected in the model, although providing inconclusive evidence of an association (Ϫ11.39 [Ϫ22.69 to Ϫ0.10] P ϭ .048). In further exploratory analysis we fitted a model excluding BMI but including age, season, and relationship status, in which self-reported general health was found to be more strongly associated with Sal-T (Ϫ19.01 [Ϫ30.48 to Ϫ7.54] P ϭ .001; data not shown).

Specific health conditions
Cardiovascular disease (including hypertension) was reported by 19% of men in our sample, and was associated with lower mean Sal-T in men, independently of age (Ϫ18.06 [Ϫ29.43 to Ϫ6.68]) ( Table 4). There was also an association with currently taking medication for depression (Ϫ24.56 [Ϫ48.33 to Ϫ0.79], reported by 4% of men), but not with current depressive symptoms (10% of men screened positive for depressive symptoms in the past 2 weeks). After adjustment for age, there was at best weak evidence of an association with self-reported doctor-diagnosed diabetes (reported by 6% of men in our sample). ; P ϭ .020). There was no evidence of associations between Sal-T and any of these conditions among women.

Summary of findings and comparison with other studies
This study is the first to show associations between Sal-T and health in a large national probability-sample survey of men and women, across a wide age range. In men, we found significant age-independent associations between lower Sal-T and higher BMI, poorer self-reported general health, mobility problems, longstanding illness, and comorbid conditions (cardiovascular disease, treatment for depression). The association between Sal-T and self-reported general health in men was attenuated after adjustment for BMI, suggesting that the relationship is at least partly explained by obesity. We found no associations between Sal-T and health factors in women, except smoking, with higher Sal-T among women who smoked.
The present finding of an age-related decline in Sal-T that persisted even after adjusting for health and demographic factors is consistent with previous serum studies that found not only serum total-T, but also serum free-T, declined across an age range in women (9) and men similar to Natsal-3 (24). Others, however, have argued that the age-related decline in serum total-T may entirely be explained by declining health (6). This inconsistency may partly be explained by the age-related increase in SHBG, which attenuates the age trend in total serum-T, but not serum free-T (5) or Sal-T.
Our findings of associations with health-related factors in men are largely consistent with evidence from serum press.endocrine.org/journal/jcem studies. There is a large body of evidence that, in men, obesity is strongly associated with lower serum-T, independently of age (3). Associations have also been reported between lower serum-T and insulin resistance and diabetes, preclinical indicators for cardiovascular disease, cardiovascular events, physical frailty, and increased mortality (3,5,25,26). Cross-sectional data cannot shed light on the direction of these relationships but longitudinal studies have found that obesity leads to decreases in T, and weight loss increases T levels in obese men (27), although there is also evidence of bidirectional associations (3).
Longitudinal serum-T studies have shown that low T precedes cardiovascular events (25). The mechanisms of this association are unclear and may involve low T affecting several cardiovascular risk factors, central adiposity, and inflammation (28). Unlike most serum-T studies we did not find an age-adjusted association with self-reported diabetes for men with P ϭ .0932 indicating at best weak evidence of an association, which did not persist after adjusting for other confounding factors including BMI. Although prevalence of diabetes in our sample was similar to national estimates (29), the absolute number of men with diabetes in our sample was relatively small, thus limiting our power to detect an association. However other research has shown that the apparent association between diabetes and low serum total-T, but not free-T, may be Abbreviations: unwt, unweighted; wt, weighted.
Variables were grouped into a hierarchy of domains as follows: 1. Age, season, region; 2. Relationship status and family; 3. BMI; 4. General health and function. Lifestyle factors (smoking, drinking, drug use) were not included in the adjusted analysis given lack of associations seen in ageadjusted analysis.
The variables were entered in these groups in order, and including any variables found to be significant from previous domains, into forwards stepwise linear (interval) regression models to generate adjusted coefficients. The significance criteria for retention in the model was P Ͻ .1.
confounded by obesity and low SHBG, which is in line with our findings (30). We found no association with current depressive symptoms but did observe an association with treatment for depression. Little research exists on the effects of antidepressants on the hypothalamic-pituitary-gonadal axis, although one study has found higher Sal-T levels among men and women using selective serotonin reuptake inhibitors, which contrasts with our results (31). We examined associations between Sal-T and several demographic factors to address potential confounding of the associations with health. A previous study found ethnic variation in T levels; however, we were unable to examine this due to small numbers of participants in ethnic minority groups. The findings of this study only partly concur with those from (generally smaller) studies that have reported lower T among men and women in established relationships (32) and parents, especially those actively involved in childcare (33,34). We found associations with relationship status for men only, and no associations with parenthood for either men or women; however, our measures of parenthood capture a broad range of circumstances regarding children's age and parents' involvement in child rearing, which may explain this apparent discrepancy. We found no association between Sal-T and smoking, alcohol consumption, or drug use in men. Previous serum-T studies in men have generally, although not always consistently, shown smoking to be associated with increased total-T, and studies of the associations with free-T have yielded mixed results (35). Smoking, via direct effects on liver function, increases SHBG levels, which may explain why total-T is increased in smokers yet in our study we did not find an association with Sal-T in men (35). The evidence regarding whether T is associated with alcohol consumption is also mixed (26,36), and few studies have examined associations with other drugs.
Previous research about the health correlates of T in women has not only yielded inconsistent findings, but has largely been carried out within narrow age ranges, and using suboptimal measures of T (14). We found no evidence of associations between Sal-T and general health indicators, or specific conditions or medications in women. We did find a positive association with smoking, which is consistent with other studies (11,37). We also found lower mean Sal-T among women who had taken HC, which can be explained by the combined effects of a direct inhibitory effect on ovarian androgen synthesis, an increase in SHBG concentration, and an inhibitory effect on adrenal androgen production (38). The association between Sal-T and smoking in women became nonsignificant after excluding women who had taken HC; however, the effect estimate was similar, so it is likely that this change in statistical significant is due to the reduction in sample size and resulting increase in confidence intervals, rather than a true difference between these groups of women.
Our findings regarding the different relationship with health and obesity for men and women may relate to the different sites of production and mechanisms of regulation. Among women, T is produced from the adrenal glands, regulated by adrenocorticotropic hormone, as well as the ovaries, regulated by gonadotropins, and there are menstrual cyclical fluctuations of ovarian steroids, whereas in men T is produced predominantly from the testes with only a very minor contribution from the adrenals. The mechanisms underlying the relationship between obesity and low T in men remain unclear, although the potential importance of circulating inflammatory cytokines from visceral adipose depots is gaining credence (39,40).

Strengths and limitations
A key strength of this study is the highly sensitive and specific Sal-T assay, enabling accurate measurement in women as well as men-although there is some systematic positive bias due to salivary protein binding among women (19)-and permitting measurement of T on a large-scale probability-sample survey across a wide age range. Although less invasive than serum, large-scale home-based collection of saliva has presented a number of challenges including coping with the diurnal variation in T levels, preventing contamination, ensuring prompt re- Abbreviations: unwt, unweighted; wt, weighted. a Participants were asked whether they had often been bothered by feeling down, depressed, or hopeless in hte past 2 weeks, and whether they had often been bothered by little interest or pleasure in doing things in the past 2 weeks, using a validated two-question patient health questionnaire (PHQ-2). b Ratio of geometric means, obtained from exponentiated age-adjusted linear regression coefficients of log-transformed data for women.
ceipt at the laboratory before deterioration, and minimizing nonresponse bias. Considerable attention was paid to the development of protocols for sample collection, with extensive validation and piloting (20), yet although 71.0% of participants agreed to provide a sample, useable samples were received from only 45.0% of those invited, highlighting the challenge of obtaining self-collected and self-posted samples. This response rate is similar to community-based serum T studies (41,42), and the response rate to the survey overall was similar to other major British social surveys. However, there were some systematic differences in the characteristics of those who returned a valid saliva sample, for example older participants were more likely to give a sample; therefore, to minimize potential nonresponse bias, both to the survey and to the saliva sample, we applied statistical weights during analysis (20). The health data collected were self reported and are therefore reliant on knowledge of conditions and medications, and accurate reporting. This may particularly affect BMI based on self-reported height and weight, given that people tend to underestimate weight and overestimate height. However, previous studies have shown self-reported height and weight to be sufficient for examining associations in epidemiological studies (43). As a sexual health survey, only a limited number of questions about general health could be asked; therefore, we were unable to look at associations with some factors that may have been of interest such as frailty, osteoporosis, or sleep disturbance. We were also unable to measure preclinical disease indicators.
Only one sample was collected from each participant and so we were unable to take into account intra-individual variation in T levels, which may be particularly relevant for premenopausal women given that T varies throughout the menstrual cycle (44). However, some have argued that it is unnecessary to control for menstrual variation, given the relatively small effects compared with, for example, diurnal variation or individual differences (44). This is consistent with our earlier validation work in which we did not find significant within-individual differences when samples were taken at weekly and monthly intervals (17). A small number of men and women (n ϭ 20 and n ϭ 37, respectively) included in our analysis reported receiving cancer treatment in the past year, and as the nature of the cancer or treatment was unknown, this could have affected T levels for some. However, given the small numbers this is unlikely to have affected our findings overall.

Implications for clinical research, policy, and additional research need
The findings presented in this paper provide crucial background for future research into the relationship be-tween Sal-T and sexual function and sexual behavior, as well as being important in their own right for understanding associations between Sal-T and health factors. Our findings are broadly consistent with previous research using serum, and where differences exist it is not always clear whether these are due to differences in the measure used (serum total-T, free-T, or Sal-T), or due to other differences in the study population and design. Further observational research using reliable saliva T measurements linked to a broader range of clinical correlates would strengthen the evidence base in this respect.
There are concerns about inappropriate marketing and use of TRT for men, particularly in the United States (7). Our cross-sectional finding of lower Sal-T among men with poorer health does not imply causality, nor does it indicate treatment. Although longitudinal research has shown that low T precedes poor health outcomes (25) the benefits of TRT in the general population remain unclear. Further research is needed before conclusions can be reached regarding the nature of the relationship between T and ill health, and the risks and benefits of intervention.
Our finding of an independent age-related decline in Sal-T suggests that reproductive senescence in men, as in women, is not solely the consequence of poor health. The clinical significance of this merits further investigation using Sal-T as well as serum-T measurements. The application of Sal-T measurement in future research should make a significant contribution toward clarifying the role of low T in health and ageing in men and women. The views expressed in this publication are those of the author(s) and not necessarily those of the NHS, the National Institute for Health Research or the Department of Health.