{'fraction_non_alphanumeric': 0.015604681404421327, 'fraction_numerical': 0.002600780234070221, 'mean_word_length': 5.875, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Detecting inhibition of coxsackievirus replication by measuring DNA synthesis – effect of proteinase inhibitors

The replication of picornaviruses can be monitored by microscopic examination of the cytopathogenic effect. We found that measuring cellular DNA synthesis gave a more objective and reliable estimate of the viral effect on the cells. Furthermore, by simultaneously measuring DNA synthesis in uninfected cells, we obtained a sensitive indication of whether the agents tested influenced cellular activity. The method was employed to investigate the effect of various proteinase inhibitors on the replication of coxsackie‐B3 virus in HEp2 cells. Certain inhibitors of metallo‐proteinases had a limited, but consistent, protective effect against viral activity.