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Estimation of the Ca2+ binding by microsomal membranes from pig coronary artery using a calcium ion selective electrode.

To estimate the Ca2+ binding of microsomal membranes from pig coronary artery a modified potentiometric method was used. With this method it was possible to follow directly changes in the Ca2+ ion activity of the incubation medium down to 10(-8) moles. Compared with the Ca45-filter technique the electrometrically measuring device built up was beside the obvious advantages of a directly indicating method more fast, simple and hightly sensitive.