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Phytochemicals, biological activity, and industrial application of lotus seedpod (Receptaculum Nelumbinis): A review

Lotus (Nelumbo nucifera Gaertn.) is a well-known food and medicinal plant. Lotus seedpod (Receptaculum Nelumbinis) is the by-products during lotus products processing, which is considered as waste. Numerous studies have been conducted on its phytochemicals, biological activity and industrial application. However, the information on lotus seedpod is scattered and has been rarely summarized. In this review, summaries on preparation and identification of phytochemicals, the biological activities of extracts and phytochemicals, and applications of raw material, extracts and phytochemicals for lotus seedpod were made. Meanwhile, the future study trend was proposed. Recent evidence indicated that lotus seedpods extracts, obtained by non-organic and organic solvents, possessed several activities, which were influenced by extraction solvents and methods. Lotus seedpods were rich in phytochemicals categorized as different chemical groups, such as proanthocyanidins, oligomeric procyanidins, flavonoids, alkaloids, terpenoids, etc. These phytochemicals exhibited various bioactivities, including ameliorating cognitive impairment, antioxidation, antibacterial, anti-glycative, neuroprotection, anti-tyrosinase and other activities. Raw material, extracts and phytochemicals of lotus seedpods could be utilized as sources for biochar and biomass material, in food industry and as dye. This review gives well-understanding on lotus seedpod, and provides theoretical basis for its future research and application.

Phytochemicals, biological activity, and industrial application of lotus seedpod (Receptaculum Nelumbinis): A review Introduction Nelumbo nucifera Gaertn. (also named as lotus), belonging to the mono-generic family Nelumbonaceae, is widely distributed in Asia, Americas and Oceania (1). Lotus has been cultivated as vegetable, functional food, and herb medicine for over 2,000 years (2). According to the phytomorphology, it can be divided into different parts, such as leaf, flower, stamen, rhizome, seed, seedpod and plumule (2). Almost every part can be used, and most of them are recorded in the "Chinese Pharmacopeia" (3). Among them, rhizome and seed are the main edible parts, which are popularly consumed as vegetables or functional foods due to their delicious taste and great nutritive and non-nutritive values (2).
Lotus seedpod (Receptaculum Nelumbinis) is the mature receptacle of lotus house (seen in Figure 1), and is usually regarded as by-products during lotus seed processing. As one of the non-edible parts of lotus, its production is almost equivalent to the edible parts (4). In the Traditional Chinese medicine, lotus seedpod can be used for treating excessive menstrual bleeding and as hemostatic (5). According to the 2015 edition of "Chinese Pharmacopeia, " lotus seedpod charcoal (a processed product of lotus seedpod) has the usage in treating hemorrhage, urine blood, hemorrhoids bleeding, postpartum stasis, lochia, etc. However, this charcoal has gradually faded out of the market as the progress of medical science and technology. On account of lack adequate understanding on lotus seedpods, most of them are generally considered as wastes and are thrown away in the open or incinerated. This not only causes significant wastes of resources, but also brings heavy pollutions to environment. In this light, reusing the sources lotus seedpods into high value-added products is of great value.
In the past decades, more and more attention has been paid to phytochemicals, biological activity and industrial application of lotus seedpods. A variety of phytochemicals have been derived and identified from lotus seedpods, including proanthocyanidins (6), oligomeric procyanidins (7), polyphenols (8), flavonoids and others (9). Numerous studies have demonstrated that extracts and phytochemicals of lotus seedpods possessed various biological activities, such as ameliorating cognitive impairment (10)(11)(12), antioxidation (8,9,13,14), antibacterial (15)(16)(17), anti-glycative (7,18,19) and anti-diabetes (20). Due to the good biological activities and superior physicochemical property, raw material, extracts and phytochemicals of lotus seedpods have been increasingly applied in food and other industries (21)(22)(23). Despite of multiple researches have been carried out to lotus seedpods, the information concerning it was scattered. In contrast, summary on other parts of lotus like seeds (1,24) and leaf (25) have been reported. However, the review on lotus seedpods is rare.
Hence, in this article, the information on phytochemicals, biological activity and industrial application of lotus seedpods was systematically summarized. Meanwhile, the future study trend about lotus seedpods was proposed. This work gives wellunderstanding on lotus seedpod, and provides theoretical basis for research as well as exploitation and application of it, which is helpful to the protection of lotus seedpod sources. Photographs of planted lotus (A), fresh lotus seedpod (B) and sun-dried lotus seedpod (C) taken by Jian-Lin Shen in August, 2022.
Different preparation methods produced differences in the chemical structures of phytochemicals from lotus seedpods. In the study taken by Wu et al. (35), five compounds have been respectively obtained from n-BuOH extracts of lotus seedpods by silica gel column chromatography with different CH 2 Cl 2 -MeOH elution ratios and Sephadex LH-20 column chromatography. These five compounds have different chemical structures, which have been identified to be hyperoside, isoquercitrin, quercetin-3-O-β-D-glucuronide, isorhamnetin-3-O-β-D-galactoside and syringetin-3-O-β-D-glucoside. On the other hand, extracts of lotus seedpods from different production places have been found to contain different chemical compositions. Another study of Wu et al. (40) has indicated that 50% ethanols of 20 lotus seedpods samples, collected from different regions (Fujian, Jiangxi, Zhejiang, Beijing, Jiangsu, Hubei, Hebei, and Hebei) of China, showed different contents of these five compounds. An investigation conducted by Liu et al. (50) has demonstrated that hyperoside and isoquercitrin in methanol extracts of 11 lotus seedpods samples, from different localities of Jianning County, Fujian province, China, exhibited different chemical fingerprints. Otherwise, different sources of lotus seedpods also contained different chemical compositions. The research of Limwachiranon et al. (45) has implied that lotus seedpods from three commonly consumed cultivars (Shilihehua, Jianlian and liyebailian) showed differences in phenolic, flavonoid, and proanthocyanidins contents.
In terms of the antioxidation activity, chemical assays have indicated that water extracts from lotus seedpods had scavenging effects on DPPH, ABTS and NO 2 − radicals, and ferric reducing ability (14,29). A cell experiment has demonstrated that the extracts could dose-dependently improved the survival and function of rat pancreatic RIN-m5F cells induced by H 2 O 2 through down-regulation of apoptosis and up-regulation of autophagy, revealing as increasing protein expressions of LC3II, Atg5/12, p62, class III PI3K, Beclin-1 and p-Bad/Bad, and decreasing protein expressions of active-caspase-3 and cleavage PARP-1 along with Bax/Bcl-2 ratio (9). Regarding to anti-melanogenic effect, water extracts of lotus seedpods could inhibit melanin synthesis in α-melanocyte stimulating hormone-induced B16F10 cells and in UVB-induced mice, which involved both PKA and p38 signaling pathways (27). In the cell experiment, the extracts decreased protein expressions of p-PKA/PKA, p-p38/p38, p-CREB/CREB and MITF, and protein and mRNA expressions of tyrosinase, TRP-1 and MC1R of B16F10 cells. In the animal experiment, the extracts reduced protein expressions of tyrosinase, TRP-1, TRP-2, p-PKA/PKA and p-p38/p38 in ears of mice. As to anti-inflammatory action, water extracts of lotus seedpods exerted protective effects against LPS-induced HepG2 cells and LPS-induced mice via NF-κB and p38 signaling pathways (28). In the cell experiment, the extracts could lessen mRNA expressions of IL-6, COX-2 and iNOS, and protein expressions of COX-2, iNOS, NF-κB, IKK, p-IκB/IκB, p-p38/p38, TLR4 and MyD88 of HepG2 cells. In the animal experiment, the extracts could decrease protein expressions of COX-2, iNOS, NF-κB, IKK, p-IκB/IκB and p-p38/p38 in liver of mice. To hepatoprotection effect, water extracts of lotus seedpods reduced lipid accumulation and lipotoxicity in oleic acid-induced HepG2 cells through anti-apoptotic and anti-autophagy mechanisms, showing as adding Bcl-2 protein expression, and lowering protein expressions of LC3-II/LC3-I, Atg5/12, active-caspase-3/8/9, cleaved PARP, Bax and mitochondrial membrane depolarization, as well as Bax/Bcl-2 ratio (26). Additionally, water extract from lotus seedpods had anti-proliferation effect on HepG2 cells (14).
On the other hand, subcritical water extracts from lotus seedpods have been determined to reveal antioxidation activity, showing as scavenging DPPH, ABTS and NO 2 − radicals and ferric reducing ability, and anti-cancer effect against cell proliferation of HepG2 cells (14).
With regard to antioxidation effect, chemical assays have demonstrated that ethanol extracts from lotus seedpods exhibited scavenging effects on DPPH, ABTS, OH, O 2− and H 2 O 2 radicals, ferric reducing ability and metal ion chelating activity (8,29,34,35). A cell experiment has shown that the ethanol extracts revealed cytoprotection on H 2 O 2 -induced RAW264.7 cells and reduced MDA level (34). In terms of anticancer activity, ethanol extracts from lotus seedpods exerted anti-proliferation effect on HepG2, LNcap, A549 and H460 cells (8,32). Thereinto, the extracts induced cell apoptosis of A549 and H460 cells via downregulation of Axl, illustrating as increase of cleavage PARP and γ-H2AX protein expressions, and decrease of PARP protein expression as well as Axl protein and mRNA expressions (32). Regarding to anti-melanogenic effect, inhibition of the extract on melanin synthesis in αmelanocyte stimulating hormone-induced B16F10 cells was related to downregulation of activity and protein expression of tyrosinase (33). As to anti-inflammatory action, MAPKs, NF-κB and Nrf2/HO-1 pathways were regulated by the extracts to LPS-induced RAW264.7 cells, which showing as increasing protein expressions of Nrf2 and HO-1, and decreasing protein expressions of iNOS, COX-2, p-p38/p38, p-ERK/ERK, p-JNK/JNK, p65 and p-p65 (31).

Biological activity of other extracts from lotus seedpods
Methanol, glycerol, n-hexane, chloroform, ethyl acetate, butanol and acetone-water extracts from lotus seedpods have been shown to possess antioxidation (13, 29, 36), antiirradiation (37) and cardioprotection effects (30). The extracts obtained by these solvents (except acetone-water) exerted antioxidation activities in scavenging DPPH and ABTS radicals and ferric reducing ability (13, 29, 36). Acetone-water extract of lotus seedpods showed anti-irradiation effect against 60 Co irradiation-induced mice, behaving as increments of survival time, activities of SOD, CAT and GPX in liver, levels of white blood cells, red blood cells, platelets and hemoglobin, and spleen weight and index, and reduction of LPO level in liver and chromosomal aberrations in the bone marrow, to

Comparison on biological activities of different extracts from lotus seedpods
Extracts from lotus seedpods acquired with different solvents and methods had distinctions in biological activities. Antioxidation activities of 80% ethanol, n-hexane, chloroform, ethyl acetate, butanol and water extracts from lotus seedpods have been investigated in the study of Kim and Shin (29). The results suggested that these extracts had different flavonoid and proanthocyanidin contents, and the scavenging activities on DPPH and ABTS radicals and ferric reducing ability for them were 94.319, 33.387, 85 (14) have manifested that subcritical water extract (SWE) and hot water extract (HWE) from lotus seedpods contained different polyphenol (815.4 and 785.6 mg GAE/g DW, respectively) and flavonoid (1012.05 and 932.56 mg RE/g DW) contents. The SWE showed significantly higher scavenging activities of DPPH, ABTS and NO 2 − radicals and ferric reducing ability, along with antiproliferative activity on HepG2 cells, as compared to the HWE.
Moreover, the study taken by Bao et al. (36) has made comparisons on the antioxidant activities of extracts from lotus seedpods by four extraction means, including ultrasonic coupled with glycerol (UG), ultrasonic using water (UW), water bath incubation with glycerol (WG) and water bath Biological activities of extracts (A), proanthocyanidins (B), and oligomeric procyanidin (C) from lotus seedpods.
incubation using water (WW). The results displayed that the extract gained by UG had the relatively highest scavenging activities on DPPH and ABTS radicals and ferric reducing ability. Another investigation of them implied that extract from lotus seedpods obtained by gas-assisted combined with glycerol approach exhibited obvious higher scavenging effets on DPPH and ABTS radicals along with ferric reducing ability than that acquired by WG (13).
Animal studies have indicated that proanthocyanidins from lotus seedpods ameliorated cognitive impairment of Dgalactose-(12), extremely low frequency electromagnetic fields- (11,56,57), scopolamine-(52-55) or alcoholinduced mice (52), senescence-accelerated mice (61), and aged rats (58)(59)(60). In terms of D-galactose-induced mice model, the ameliorating cognitive impairment action of the proanthocyanidins correlated with reverse of oxidative damage, prevention of Aβ overproduction and suppression of NO production, appearing as enhancements of SOD and GPX activities in brain, reductions of Aβ 1−42 , NO and MDA levels and AchE, MAO-B, tNOS and nNOS activities in brain, and declinations of nerve cell apoptosis and p53 protein expression in hippocampus (12). Regarding to extremely low frequency electromagnetic fields-induced mice model, Ca 2+ /CaMK II/CREB/BDNF and DG/PKC/MAPK signaling pathways involved in the ameliorating cognitive impairment effect of the proanthocyanidins (11,57). The proanthocyanidins increased protein expressions of CaMKII, PKCα, BDNF and p-ERK1/2, and decreased concentrations of Ca 2+ , IP3, DAG, glutamate, GABA and [Ca 2+ ] i and protein expressions of Gi, PKA, PKCβ, PP2B, ASK1, NR2B, p-CREB and p-JNK1/2, in hippocampus of mice. Moreover, the action was also related to improvement of antioxidant status, showing as increasing activities of SOD, CAT and GPX, and decreasing levels of MDA and NO and activity of NOS, in serum and hippocampus of mice (56). As to scopolamine-or alcohol-induced mice model, the ameliorating cognitive impairment action of the proanthocyanidins was correalted with improvement of antioxidant ability and cholinergic activity, exhibiting as elevating T-AOC level and activities of SOD and GPX, lessening levels of MDA, MAO-B, AchE and NO, activities of MPO, AchE, tNOS, nNOS and iNOS, and nNOS mRNA expression, in hippocampus, brain, serum and/or colon (52-55). For senescence-accelerated mice model, the ameliorating cognitive impairment effect of the proanthocyanidins was in connection with boost of antioxidant level, reflecting as enhancing GSH level and SOD and GPX activities, and reducing NO and MDA levels and nNOS and total NOS activities in brain and/or serum (61). To aged rats model, the ameliorating cognitive impairment effect of the proanthocyanidins correlated with changes of NO system (60), activation of hippocampal CREB through ERK-mediated signaling pathway (59) and rejuvenation of antioxidant and cholinergic systems (58). The proanthocyanidins elevated levels of GSH, T-AOC, AchE and Ach, activities of CAT and GPX, protein expressions of p-CREB, BDNF, p-ERK42/ERK42, p-ERK44/ERK44 and iNOS, and mRNA expressions of iNOS and BDNF, and declined levels of NO and MDA and activities of tNOS and iNOS in hippocampus and/or cerebral cortex of rats.
A chemical assay has shown that proanthocyanidins of lotus seedpods exhibited antioxidation activity in scavenging DPPH and ABTS radicals along with ferric reducing ability (62). A further cell experiment has suggested that the proanthocyanidins could relieve oxidative damage in H 2 O 2induced HUVECs, appearing as increasing activities of SOD and GPX and production of NO, and decreasing levels of MDA and ET-1 (62). Animal experiments have proven that the proanthocyanidins exerted antioxidation action in aged rats (64) and extremely low frequency electromagnetic fields-induced mice (63). In terms of aged rats model, the proanthocyanidins promoted the activities of SOD, CAT and GPX and level of GSH, and lessened TBARS content, in serum, heart, liver, kidney, lung or muscle (64). Regarding to extremely low frequency electromagnetic fields-induced mice model, the proanthocyanidins aggrandized activities of SOD, CAT, GPX, GR and GST, and lowered MDA level, in serum and cerebral cortex (63).
Animal experiment has demonstrated that proanthocyanidins of lotus seedpods reflected retinal protection against light exposure-induced rats through anti-oxidative stress, anti-apoptosis and neuroprotective effects, displaying as improving activities of SOD and GPX and mRNA and protein expressions of Bcl-2, and lowering retinal apoptosis, mRNA expressions of caspase-3, p53 and Bax, and protein expressions of pro-caspase-3, cleaved caspase-3, p53 and Bax (6). The proanthocyanidins exerted anti-insomnia effect to rats by regulating NO/ADMA/DDAH pathway, showing as increasing levels of 5-HT, GABA and NO, and protein and mRNA expressions of DDAH1, DDAH2 and nNOS, and decreasing levels of NE, Glu, ADMA and 8-isoprostane, in brain (67). Proanthocyanidins from lotus seedpods exhibited immunomodulatory effect against extremely low frequency electromagnetic fields-induced mice, and increased protein expressions of IL-2, IL-6, IL-10, INF-γ and Bcl-xl, and DNA contents, and decreased protein expressions of TNF-α and caspase-3/9 along with Bax/Bcl-2 ratio and apoptotic splenocytes in spleen (69). Otherwise, cell experiment has showed that proanthocyanidins from lotus seedpods possessed anti-cancer activity in HepG2 cells through inducing autophagy and ROS generation (68).
In addition, there are some connects between the different bioactivities of proanthocyanidins from lotus seedpods. The retinal protection of proanthocyanidins against light exposureinduced rats was realized partly through their antioxidation and neuroprotection activities (6). Ameliorating cognitive impairment action of proanthocyanidins was reported to be related to their antioxidation effects in D-galactose-, scopolamine-, alcohol and scopolamine, senescence-accelerated or extremely low frequency electromagnetic field exposureinduced mice (12, 52-54, 56, 61) as well as cognitively impaired aged rats (58). The neuroprotection of proanthocyanidins was associated with their antioxidation action in extremely low frequency electromagnetic field-induced primary cultured hippocampal neurons (66) and methyl mercuric chlorideinduced neuron/astrocyte co-cultured cells (48).
Oligomeric procyanidins of lotus seedpods showed antibacterial activity in vitro and in vivo. The oligomeric procyanidins had the minimal inhibitory concentrations on Escherichia coli K88ac, F18ac, 10899 and BL21 as 0.80, 1.20, 1.25, and 1.25 mg/mL, respectively (17,65). Moreover, synergistic effect was observed between the oligomeric procyanidins and water-soluble Poria cocos polysaccharides or carboxymethyl pachyman in inhibitory effect on Escherichia coli (16, 17). The antibacterial mechanism of oligomeric procyanidins on Escherichia coli has been disclosed to be increments of extracellular alkaline phosphatase, ROS production, activities of SOD and CAT and mRNA expressions of sodA, soxR, oxyR and oxyS (15). Further study has indicated that the oligomeric procyanidins reflected antibacterial activity against high-lactose diet-induced mice by enhancing abundances of Lactobacillus and Bifidobacterium, and lessening populations of Escherichia coli and Enterococcus in feces (15). On the other hand, the oligomeric procyanidins ameliorated intestinal injury against enterotoxigenic Escherichia coli-infected diarrhea mice (70). This effect was related to the modulation of TLR4-MAPK signaling pathway, reflecting as increasing protein and mRNA expressions of ZO-1, claudin-1 and occludin, and decreasing protein expressions of p-p38, p-JNK1/2 and p-ERK1/2 and RNA expressions of TNF-α, IL-8, IL-1β, IL-6, CD14, TLR4, p38 and NF-κB in jejunum/ileum.
Animal experiment has shown that oligomeric procyanidins from lotus seedpods possessed anti-diabetes action against streptozotocin-induced mice by attenuating mTOR signaling and enhancing glucose homeostasis (20). This action was related to enhancement of protein expressions of GLUT2, GK, p-AKT, UCP-1 and GLUT4 and mRNA expressions of HK II, PFK and PK, and reduction of protein expressions of mTOR, p66 Shc , PKCβ, FoxO1a, p-FoxO1a and GLUT1 and mRNA expressions of PEPCK, G-6-Pase, SREBP-1c, ACL, ACC1, FAS, SCD1 and S6K1, in liver, skeletal muscle, white adipose tissue and/or brown adipose tissue. Chemical assays have revealed that the oligomeric procyanidins had antioxidation action in scavenging ·OH, O 2− and H 2 O 2 radicals (5). Animal investigation has suggested that the oligomeric procyanidins regulated the lipid profile of high fat/sucrose diet-induced rats by suppressing the lipogenesis-related gene expressions, such as SREBP-1c, FAS, ACC1, PPARγ and CD36, and elevating phase II drug metabolism enzyme SULT2B1b gene expression (38).
Additionally, some connects between the different bioactivities of oligomeric procyanidin could be found. Wu et al. (7,18,19,72) have argued that anti-glycative activity of oligomeric procyanidins from lotus seedpods is positively correlated to their antioxidant capacities. The study taken by Tang et al. (70) has indicated that the antioxidant mechanism involved the ameliorating intestinal injury activity of oligomeric procyanidins from lotus seedpods on Enterotoxigenic Escherichia coli infected diarrhea mice.
Applications of raw material, extracts and phytochemicals of lotus seedpods
As a source of biochar, lotus seedpods can be used to constitute sensor (74), absorber (21,73,75,81) and detector (76). A portable, flexible, outdoor and inexpensive sensing platform for hyperin has been established by lotus seedpods biochar and molybdenum disulfide, using a green cohydrothermal approach (74). Lotus seedpod-derived biochar can be used for producing available and effective biosorbents for cadmium (73), methylene blue (81) and 17 β-estradiol (21), along with Co 3 O 4 microwave absorbent (75). Otherwise, a carbon quantum dots was synthesized on the basis of lotus seedpod by hydrothermal synthesis method, which could be utilized for Fe(III) detection (76).
In terms of using for biomass material, lotus seedpod is high-stable electrode material for supercapacitors (22,78,80). Meanwhile, lotus seedpod-derived hard carbon with hierarchical porous structure is a stable anode for sodium-ion batteries (79). Otherwise, an efficient metal-free catalyst derived from lotus seedpod exhibited excellent oxygen reduction reaction (77).
Inhibition of AGEs formation is an important application for lotus seedpod oligomeric procyanidin in food systems, such as bovine serum albumin-glucose (43,72), lactose-lysine (44) and yogurt (86) systems. Meanwhile, addition of lotus seedpod oligomeric procyanidin increased the growth of Lactobacillus plantarum, titratable acidity, DPPH scavenging effect, solid-like properties, hardness, adhesiveness, gumminess and chewiness of yogurt (86). Moreover, lotus seedpod oligomeric procyanidin and its three monomers including catechin, epicatechin and epigallocatechin gallate could inhibit AGEs release from glycated casein during gastrointestinal digestion (49). At the same time, lotus seedpod oligomeric procyanidin and catechin could also enhance the scavenging effects of digestive fluid on DPPH, OH and ABTS radicals and its ferric reducing ability (19). On the other hand, lotus seedpod oligomeric procyanidin is beneficial to the storage and process of food. Lotus seedpod oligomeric procyanidin could decline the peroxide values of lard or soybean oil (5). Lotus seedpod oligomeric procyanidin possessed inhibitory effect on the retrogradation property of rice starch (23).
Beside, oligomeric procyanidin extracted from lotus seedpod has been used to dye the tussah silk fabric (87).

Conclusion and perspectives
Lotus seedpod is a promising food and medicine source. Extracts of lotus seedpods obtained by non-organic and organic solvents exerted antioxidation, anti-cancer, anti-melanogenic, anti-inflammatory, anti-irradiation, cardioprotection and hepatoprotection activities. Meanwhile, extraction solvents and methods have influences on the activities. Diversity phytochemicals are responsible for these bioactivities, such as proanthocyanidins, oligomeric procyanidins, flavonoids, alkaloids, terpenoids, organic acids, steroids, esters and others. Some of phytochemicals have been well-identified by modern analytical techniques, and chemical structures of some phytochemicals have been proposed. Moreover, chemical assays as well as cell and animal experiments have demonstrated that phytochemicals (especially proanthocyanidins and oligomeric procyanidins) from lotus seedpods exhibited broad-spectrum biological activities, including ameliorating cognitive impairment, antioxidation, antibacterial, antiglycative, neuroprotection, anti-tyrosinase, retinal protection, anti-insomnia, anti-cancer, immunomodulatory, ameliorating intestinal injury, anti-diabetes, regulating lipid homeostasis, anti-inflammatory and α-glucosidase inhibitory effects. Furthermore, raw material, extracts and phytochemicals of lotus seedpods have been applied as sources for biochar and biomass material, in food industry, and as dye. In contrast, other parts of lotus like seeds, stems, leaves, flowers, epicarps, plumules, stamens, petals and rhizomes have been reported to contain phytochemicals included flavonoids, glycosides, alkaloids, monosaccharides, essential oils, organic acids, chlorophylls, sesquiterpenoids, steroids, sapogenins, etc (2). Biological activities of these parts of lotus have been summarized as anti-obesity, antioxidant, cardiovascular, hepatoprotective, hypoglycemic, antimicrobial, lipolytic, antiamnesic, anti-inflammatory, antithrombotic, anti-proliferative, memory-improving, sedative, immunoregulatory and antiviral activities (2). These parts of lotus can be applied in health food industry and medicine (2). Therefore, to lotus industry, lotus seedpods are worth exploiting for acquisition of novel phytochemicals, enrichment of more biological activities and new attempts of industrial applications.
However, there are some issues should be resolved in future: (1) the phytochemicals from lotus seedpod is mainly prepared using conventional solvent method, more attempts should be made to novel means; (2) although many phytochemicals with small molecular weight have been isolated and identified from lotus seedpods, the exploitations of those (like proteins and polysaccharides) with large molecular weight have been rarely done; (3) there is little information on digestion, absorption, distribution, structure-activity relationship, metabolism of phytochemicals from lotus seedpod, which need to be disclose with more work; (4) the current researches on biological activities of phytochemicals from lotus seedpods mostly foucused on proanthocyanidins and oligomeric procyanidin, the biological activities of other phytochemicals from lotus seedpod; (5) biological activities of extracts and phytochemicals from lotus seedpods have been indicated in chemical assays as well as cell and animal experiments, but have not been demonstrated by clinical research; (6) the action mechanisms about the relevant activities of extracts and phytochemicals from lotus seedpods are unclear as they have been preliminarily explored.
Author contributions Y-FW: conceptualization, investigation, visualization, and writing-original draft. Z-CS and JL: investigation and visualization. TL, J-LS, and X-FL: investigation. Y-PL: project administration and funding acquisition. WZ: writingreview and editing. QZ: funding acquisition. X-YW: project administration, writing-review and editing, supervision, and funding acquisition. All authors contributed to the article and approved the submitted version.

Funding
This work was gratefully supported by the Science and Technology Research Project of Jiangxi Provincial Frontiers in Nutrition 13 frontiersin.org