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Scanning errors in holographic tomography

In holographic tomography obtaining projections is the key part of the measurement process that enables the 3D refractive index reconstruction. The error of the illumination angle can significantly influence the reconstruction quality and alter the result degrading the reliability of the reconstruction. Thus, in this paper the impact of the scanning errors in limited angle holographic tomography with respect to two reconstruction algorithms is analyzed. The simulated errors are compared to the errors identified in the experimental system. The reconstruction errors are verified using a paramecium cell phantom at the simulation stage and with a biological object, namely a macrophage cell in the experimental part. The experimental system presented in the paper exhibits maximum expected measurement errors found in galvanometer-mirror-based holographic tomography setups.