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[Presence of spirochetes of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in the recreational area of Tarnowskie Gory and Zabrze districts in 2001-2003].

Borrelia burgdorferi sensu lato is an aethiological factor of borreliosis. Borrelia spirochete is transmitted to humans by ticks. The aim of the study was to estimate the spirochetes B. burgdorferi s.1. infection in Ixodes ricinus ticks by using PCR analysis of the flagellin gene fragment. The study was carried out in 2001-2003 in recreational areas of the Silesian Region in Poland (Tarnowskie G6ry and Zabrze administrative districts). In the 233 ticks analysed the DNA of B. burgdorferi s.l. was detected in 33 (14,2%) cases. Further comparison of spirochetes infection percentages in ticks during the three consecutive vegetation seasons was made. The investigation of B. burgdorferi s.l. detection in tick populations of recreational and endemic areas is important for the evaluation of the risk of infection.