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Identification of nosocomial infection bacteria from hospitals, and effect of some antibiotics in Wasit Province

Background: Nosocomial infections are illnesses obtained after medical care. The responsible pathogens are represented by several types of microorganisms and this study aimed to define the causative bacteria and their sensitivity to different antibiotics.  Methods: A total of 308 samples have been collected from different places and surfaces prone to bacterial contamination in the three main governmental hospitals in AL-Kut city. Firstly, bacteria were isolated and identified using the biochemical test, then antibiotic sensitivity of isolates was performed using different antibiotics for Staphylococcus haemolyticus, Acinetobacter baumannii, Serratia marcescens, and Enterobacter cloacae on Mueller Hinton agar using Kirby-Bauer disk diffusion method. Results: There were 83 (26.9%) bacterial-contaminated samples.  The most common type of isolated bacteria was Staphylococcus haemolyticus (20.5%) followed by Acinetobacter baumannii (12%) of the total bacteria. Culture and sensitivity for Staphylococcus haemolyticus bacteria showed that bacteria were resistant to all selected antibiotics reaching 92.9% for Azithromycin and 85.7% for Erythromycin. Results for A. baumannii bacteria showed bacterial resistance to all antibiotics and the highest rate (87.5%) was reported for Piperacillin. Results for Serratia marcescens bacteria showed bacterial resistance to most antibiotics except for Meropenem with 100% resistance to Augmentin and Nitrofurantoin. 