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Radioimmunoassay of 6 alpha-methyl-17 alpha-acetoxyprogesterone (MAP) in plasma of sheep during and after oral administration.

Plasma concentrations of MAP during and after oral administration were measured by a radioimmunoassay technique capable of detecting 2 pg of MAP in 0.1 ml of unextracted plasma. Four ewes were individually subjected to treatment with 50 mg MAP orally per day in 10 days. The maximum plasma concentrations of MAP during the treatment varied considerably among the 4 ewes, the highest recorded was 3.5 ng/ml and the lowest 2.1 ng/ml. The plasma level of MAP declined to a non-detectable level 5 days after the last administrations in 2 ewes, in the other 2 ewes the level was non-detectable after 6 and 7 days, respectively.