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Regulatory effects of total triterpenoid of Prunella vulgaris L on the TGF-β_1/ Smad signaling pathway against stimulated by PDGF-BB in hepatic stellate cell-T6

AIM: To investigate the inhibitory role of total triterpenoids of prunella vulgaris L.( TTP) in the proliferation,and regulatory effects on the TGF-β1/ Smad pathway in hepatic stellate cellT6( HSC-T6) stimulated by PDGF. METHODS:HSC-T6 cells were stimulated by PDGF-BB and treated with different does of TTP( 10,30,100,300, 1000 μg / m L) and PI3 K inhibitor( LY294002,10 μmol/L),the HSC-T6 proliferation was detected by MTT,the expression of mRNA and protein levels of smad2,smad3,smad7 were detected by RT-PCR and Western blot. RESULTS:As compared with the model group,TTP could inhibit HSC-T6 stimulated by PDGF-BB. Furthermore,TTP reduced the mRNA and protein expression of smad2,smad3 and increased smad7. CONCLUSION: TTP can inhibit the proliferation of HSC stimulated by PDGF-BB, the mechanism of this process may be related to the interference with TGF-β1/ Smad signal transduction pathway.