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[The structure of HeLa cell population after exposure to methylnitrosourea at different phases of culture growth].

To study the methylnitrosourea (MNU) effect on the HeLa cells culture at different stages of its growth the method of subcultivation, i. e. dissemination of cells immediately after the MNU effect, has been used followed by the study of the growth pattern of the reinoculated culture. When dissemination was carried out on the 4th, 7th and 10th day of the culture growth after the action of MNU, the highest growth inhibition effect was observed at the late stationary growth stage of the culture (the 10th day). The study of the population structure of such a culture by the method of batch cytofluorimetry has shown that MNU exerts a cytotoxic effect on cells: there is a shift in cell distribution according to the DNA content towards 4c typical of the G2/M-period of the cell cycle.