{'fraction_non_alphanumeric': 0.055191256830601096, 'fraction_numerical': 0.040983606557377046, 'mean_word_length': 4.531722054380665, 'pattern_counts': {'":': 0, '<': 5, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Apoptosis of Peripheral CD4+ T-Lymphocytes in End-Stage Renal Disease Patients Under Hemodialysis and rhEPO Therapies

End-stage renal disease (ESRD) under hemodialyses (HD) is related with a higher propensity to infections, essentially due to T-cell lymphopenia. We postulated that HD procedure affects CD4+ T cells, especially by inducing apoptotic death and that recombinant human erythropoietin (rhEPO) therapy may also play an important role in the modulation of the immune system in these patients. T-cell phenotype and apoptosis of HD patients and healthy controls were evaluated by flow cytometry using anticoagulated whole-blood samples. In 12 HD patients, these parameters were also analyzed before and immediately after HD procedure. HD patients showed a decrease in total circulating CD3+ lymphocytes, especially in CD4+ T cells (0.747 ± 0.410 vs. 0.941 ± 0.216 × 109/L, p < 0.05), which could be a consequence of the higher proportion of CD3+ and CD4+ lymphocytes in the latest stage of apoptosis (or death) and of the higher proportion of apoptotic CD4+ T cells observed in the patients immediately after HD procedure (2.91 ± 0.780 vs. 3.90 ± 1.96, p < 0.05). A positive and statistically significant correlation between CD3+ and CD4+ lymphocytes in latest stage of apoptosis (or death) with HD time was found (CD3+: r = 0.592, p < 0.01; CD4+: r = 0.501, p < 0.01). We also found a negative and significant correlation between weekly rhEPO doses and the number of CD4+ T cells (r = –0.358, p < 0.05). In conclusion, HD procedure still contributes to the development of T-cell lymphopenia, at least in part, by apoptosis induction. It was also shown that rhEPO therapy is associated with the CD4+ T-cell decline, possibly by immune modulation, eliminating atypical cells and helping to restore the CD4+ T-cell subset.