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Differential post‐translational modifications of microtubules in cells of the seminiferous epithelium of the rat: A light and electron microscope immunocytochemical study

The cells of the seminiferous epithelium of the rat testis are a rich source of microtubules and contain distinct microtubular structures such as the meiotic spindle and manchette. Microtubule diversity can be maintained by differential genetic expression of the multiple α‐ and β‐tubulin polypeptides or by tubulin monomer acetylation and detyrosination, post‐translational modifications of α‐tubulin. In the present analysis, antibodies that specifically recognize acetylated (antiacetylated), tyrosinated (anti‐Tyr) and detyrosinated (anti‐Glu) α‐tubulins were employed to examine the distribution of post‐translationally modified microtubules in the cells of the seminiferous epithelium.