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c‐myc Gene activation as a permanent trait of RSV‐infected quail cells

A high level of c‐myc gene expression was found to be a constant feature of v‐src transformation. The c‐myc gene product was analyzed in quail embryo cells transformed by different mutants of Rous sarcoma virus (RSV) that were temperature‐sensitive with respect to various parameters of v‐src function. The high‐level expression of c‐myc proved not to be temperature‐sensitive: At both permissive (35°C) and non‐permissive (41°C) temperatures, the same high levels of c‐myc gene product were found for all RSV mutants tested. This result, in agreement with earlier evidence for a v‐src‐induced proliferative stimulus which was unaffected by ts mutants at the non‐permissive temperature, shows that certain v‐src functions have not yet been fully characterized.