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Comparative and Phylogenetic Analysis of Chloroplast Genomes of Two Medicinal Species of Spatholobus.

BACKGROUND
The vine stem of Spatholobus suberectus Dunn (S. suberectus), called "JiXueTeng", has used as a significant medicine for thousands of years in China. However, reliable field identification of this medicinal plant remains problematic, which may cause serious adverse effects in the functions of the drug and may affect the clinical medication reviews.


OBJECTIVE
To ensure the use exactly of medicine and implement protective legislation, it is imperative to obtain the chloroplast (cp) genome of S. suberectus, which can be used as the valuable resources for species identification and phylogenetic analysis.


METHODS
In this study, the complete cp genomes of S. suberectus (152,173bp) and Spatholobus pulcher (S. pulcher) (151,099bp) were assembled for the first time to gain the abundant information for the genus of Spatholobus by using the next-generation sequencing (NGS) technology. And many software were used for data filtering, assembling and analyzing.


RESULTS
We found the GC content of S. suberectus and S. pulcher were closely, 35.19% and 35.37%, respectively. The noncoding region was more divergent than coding ones. Moreover, we revealed eight divergence hotspots (trnH, trnK-rbcL, trnL-rbcT, psbD-trnT, trnC-rpoB, atpI-atpH, ycf4 and trnL-rpl32) which might be used as candidate molecular markers for Spatholobus identification. The analysis of phylogenetic relationship indicated that two Spatholobus species were clustered together and was sister to Cajanus.


CONCLUSIONS
The findings of this study were conducive to species identification and phylogenetic research of Spatholobus and provided valuable resources for exploration research on substitution of S. suberectus.