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Inhibition of Cell Proliferation by Azathioprine 1

The rise in DNA synthesis in regenerating rat liver is inhibited by azathioprine (40 mg/kg). Regeneration in the presence of azathioprine occurs by hypertrophy as assessed both by an increase in the ratio of RNA or protein to DNA and by an increase in diameter of the hepatocytes. Mitoses that occur in azathioprine-treated regenerating liver result from a G2 population that is apparently stimulated by the drug. Azathioprine also inhibits DNA synthesis in normal liver, spleen, kidney, duodenum, thymus, and lymph nodes.