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Chemiluminescence Flow-Through Sensor for the Determination of Ascorbic Acid with an Immobilized Reagent

Abstract A chemiluminescence (CL) flow-through sensor for ascorbic acid, based on permanganate electrostatically immobilized on an anion exchange column is described. Ascorbic acid can be measured by the CL reaction directly with the immobilized reagent. The sensors response was linear in the range 0.05–10 µg/mL with a detection limit of 5 ng/mL. The analysis could be performed within 30 s including sampling and washing, giving a throughout of about 120 samples per hour with RSD of 1.6% for 2 µg/mL ascorbic acid. The sensor was stable for over 500 determinations and has been successfully applied to the determination of ascorbic acid in tablets and vegetables.