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Effects of injection of Salvia Miltiorrhizae on myocardial apoptosis induced by ischemia-reperfusion in rats

Objective:To study the effects of injection of Salvia Miltiorrhizae (SM) on myocardial apoptosis induced by ischemia reperfusion (I/R) in rats.Methods: Myocardial ischemia reperfusion injury model was induced by the ligation of left descending anterior coronary artery (LAD) for 30 min, followed by 120 min of reperfusion in vitro. Sixty SD rats were divided into three groups: sham group, I/R group and SM group. Apoptosis index(AI)of myocardium was detectd by TUNEL method and protein expression of Bcl 2 and Bax genes was investigated by S P immuno histochemial stain.Results: In I/R group, AI was significantly increased, whereas protein expression of Bcl 2 gene was decreased and protein expression of Baxgene was increased. SM could decrease AI ( 26.2 ± 3.4 vs 28.9 ± 3.6 in I/R group,P 0.01 ), upregulate protein expression of Bcl 2 gene(OD: 0.104 ± 0.021 vs 0.092 ± 0.014 in I/R group,P 0.05 ), and downregulate protein expression of Bax gene(OD: 0.122 ± 0.012 vs 0.136 ± 0.022 in I/R group,P 0.01 ).Conclusion: Injection of SM can protect myocardium damage induced by I/R injury. The mechanism of cardioprotection may be associated with inhibiting apoptosis of myocardium, upregulating protein expression of Bcl 2 gene and downregulating protein expression of Bax gene.