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In-vitro Acetylcholinesterase and antioxidant activity of Ficus dalhousie and Melissa parviflora Benth

Ficus dalhousie and Melissa parviflora Benth both plants have been used as Tranquiliser, Relaxants, Nervine tonic and Calming aids throughout the world. The present study was aimed to identify the antioxidant potential of the Ethyl acetate and Hydro alcoholic extract of these plants by in vitro methods. Anti-Alzheimer activity of the plant extract were screened by Acetylcholinesterase (AChE) inhibition and antioxidant by DPPH and Hydrogen oxide. The results of the assays were calculated by the percentage inhibition of these free radicals. In Acetylcholinesterase (AChE) assay inhibitory potentials of Ficus dalhousie exhibited 73.34 ± 1.12%, whereas in Melissa  arviflora it was 86.88± 2.12%. In DPPH radical scavenging assay the percentage inhibition was 77.87 ± 2.02% in Ficus dalhousie and 76.92± 1.32% in Melissa  arviflora. Hydrogen peroxide scavenging assay the percentage inhibition was 86.56 ± 1.05% in Ficus dalhousie and 80.75± 1.92% was in Melissa arviflora. In all the research assays, the extract showed a concentration dependent increase in free radical scavenging activity. The results revealed that the antioxidant effects of the plant extract might be due to the presence of phenol and flavonoid compounds. 