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A High-throughput Electron Microscopy Workflow and its Applications in Life Sciences

Electron microscopy (EM) is increasingly being used in large-scale biological projects, either for volume imaging or large area mapping. In both use cases, a need for high throughput, reliability and automation of electron microscopy highlights the importance of a new approach to large-scale imaging. In this paper, we present an innovative electron microscopy workflow that increases imaging throughput up to 100 times compared to conventional workflows. Furthermore, we demonstrate its implementation on case studies in life sciences. The innovative workflow presented here revolves around FAST-EM, a multibeam scanning electron microscope. The imaging speed of a conventional scanning or transmission EM is a limiting factor in many large-scale workflows. At the same time, increasing the throughput of a workflow without negatively impacting image quality can be difficult or impossible. To overcome this challenge, we have developed an automated multibeam scanning EM, which combines simultaneous 64-beam electron imaging with transmission detection This imaging approach presents an enormous improvement for high-throughput transmission EM of biological specimens: FAST-EM increases imaging speed and reduces operator overhead, without compromising image quality. large-scale very