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Thermostable d‐Amino Acid Aminotransferase and Its Protein Engineering

&Amino acid aminotransferase (D-AAT) plays an essential role in the biosynthesis of wamino acids, which are used as constituents of the peptidoglycan layer of bacterial cell walls, in cooperation with amino acid racemases. It has been possible to isolate a thermophilic Bacillus species producing thermostable D-AAT abundantly. This report describes cloning of the gene, its expression in E. coli, the primary structure of D-AAT, and its site-directed mutagenesis.