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Molecular Cloning of a cDNA Homologue to Arabidopsis CAB1 from Hevea brasiliensis and Its Different Expression in Mature and Senescent Rubber Tree Leaves

Based on a EST sequence encoding a deduced polypeptide with high similarity to Arabidopsis CAB1,a 1415 bp cDNA was in silico cloned from the transcriptome data of rubber tree(Hevea brasiliensis Muell.Arg.).The sequence designated as HbCAB1 contains a ORF of 798 bp with 47 bp 5 'UTR and 570 bp 3' UTR.Subsequently,a 915 bp cDNA sequence including this ORF was amplified and sequenced.Sequence analysis showed that HbCAB1 encodes a deduced protein of 265 amino acids with a theoretical molecular weight(Mw) of 28.15 kDa and isolectric point(pI) of 5.25;the protein contains a conserved light-harvesting chlorophyll a / b binding protein domain,three transmembrane helices and a C-terminal helix,a trimerization motif,as well as some chlorophyll and carotenoid binding sites.Similarity search reveals high amino acid sequence identity of CAB from different plants.For example,the similarity of HbCAB1 shares a similarity of more than 95% with the homologous protein in castor(Ricinus communis),poplar(Populus trichocarpa),or olive(Canarium album).Semi-quantitative RT-PCR analysis showed that HbCAB1 exhibits significantly lower expression in senescent leaves than the stable leaves.