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On-demand mixing and dispersion in mini-pillar based microdroplets.

The analysis and detection of ultra-trace biomarkers are often carried out in microliter droplets. Common stirring approaches have some difficulties in precise and contactless mixing and dispersion in microdroplets. In this work, an open mini-pillar-based platform that integrates with ultrasound units is developed to achieve contactless mixing and dispersion in microliter samples. On such a platform, mini-pillars can anchor microdroplets as individual microreactors, and each ultrasound unit can be remotely controlled to achieve on-demand contactless micro-stirring, which is also confirmed by mixing and dispersing of Fe3O4 nanoparticles (1 μm) in microdroplets (10 μL). Such on-demand high-throughput mixing and dispersion that integrates ultrasound mixing with microdroplet technology provides a potential robot-based platform for achieving high-throughput and ultra-trace biosensing in microliter droplets.