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Sub‐cellular localization of bestrophin‐3 in murine blood vessels

We have previously reported that TMEM16A is expressed in smooth muscle, supporting the notion that this newly suggested candidate may contribute to calcium‐activated chloride channel (CACC) conductance. However, the contribution of a previous candidate, Bestrophin, in encoding CACCs remains controversial. Here, we aimed to explore the expression and sub‐cellular localization of Best‐3 in the murine vasculature in order to ascertain its potential contribution to CACC conductance in smooth muscle. Murine blood vessels from female BALB/c mice (6–8 weeks) were isolated for electrophysiological, mRNA and protein (Western blot, sucrose fractionation and immunological) studies. We show for the first time that Best‐3 mRNA (at similar levels to TMEM16A) and protein are present in the mouse vasculature. We also provide new evidence for the presence of Best‐3 smooth muscle splice variant transcripts, and its protein localization to specific sucrose fractions suggesting compartmentalization of this protein within the cell. Consequently, we propose that Best‐3 is expressed in the murine vasculature and remains a viable candidate to contribute to a CACC complex. Supported by the British Heart Foundation.