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Clustering-Based Touching-Cells Division

Automated cell segmentation of microscopy cell images play an important role in various applications of cell images analysis. Nowadays, the automated methods for cell segmentation is still challenging in situations, not only the fuzzy microscopy cell images but also where there might be touching cells. In this paper we propose a method for identifying the touching cells. we use a combination of image segmentation, extraction of connected components, Hough transform and cluster analysis to separate the touching cells.