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The Versatility of X-ray Microscopy for Imaging Intact Plant Structures

X-ray microscopy (XRM) is becoming a valuable technique for generating three dimensional (3D) image data to complement existing laser-based and electron microscopy. This is particularly true for imaging floral and inflorescence structures that are often delicate and complicated. Examples of this in the literature used traditional electron microscopy sample preparation methods and contrast agents to produce detailed 3D volume data of a variety of floral samples [1-4]. We have modified these procedures for multiple plant tissues and organs and can regularly generate data-rich 3D volumes over a range of scales, often with significantly shorter sample preparation times.