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Abstract 15250: The Correlation Between the Amounts of Exosomes and Functional Parameters of Myocardial Perfusion Imaging in the Patients With Coronary Artery Disease


 Introduction:
 Exosomes were found to be involved in the pathophysiological process of coronary artery disease (CAD). The study investigated the relationship of the exosome amounts and imaging markers of myocardial perfusion in patients with CAD.
 
 
 Methods:
 Peripheral blood was collected from patients who had more than 70% luminal stenosis of coronary arteries on coronary angiography. Plasma exosomes were extracted by chemical precipitation and flow cytometry (CytoFLEX BB10068). Further confirmation of exosome surface markers was performed by Western blot. The myocardial perfusion imaging (MPI) of single photon emission computed tomography performed less than 6 months before coronary angiography were retrospectively reviewed. Perfusion deficits of MPI were scored visually by a nuclear medicine physician. Functional parameters of MPI were generated from gated images by QPS/QGS software (Cedars-Sinai), including ejection fraction (EF), diastolic function, shape index of heart, etc. The amounts of exosomes were correlated with the perfusion deficits and functional parameters of MPI using Spearman’s correlation. Comparison of the exosome amounts between patients with normal or abnormal functional parameters was performed by Mann-Whitney U test.
 
 
 Results:
 Exosome amounts and MPI images from 40 patients with CAD were analyzed. Exosome amounts have poor correlation with perfusion and functional parameters of MPI (all R value <0.5). However, patients with abnormal post-stress ejection fraction (<50%) had significantly higher amounts of exosome in size of 100nm and 100 to 200nm ( p = 0.046, 0.030, respectively). The mean amounts of exosome in size of 100nm and 100 to 200nm were 67793.8 ± 45498.3 and 19394.1 ± 10995.9 million particle/ml in patients with abnormal EF, compared to 43885.0 ± 24678.4 and 13604.2 ± 8381.3 million particle/ml in patients with normal EF.
 
 
 Conclusions:
 CAD patients with abnormal systolic function have increasing amounts of exosomes. Exosomes may participate in the dysfunction process of ischemic heart disease. The exact casual relationship and mechanism needs further investigation.
