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TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.

showing that if the upregulation of RUNX2 is not accompanied by the increase of COL1A1, calcium deposition does not occur and this prevents hMSC full differentiation into osteoblasts 16 . A connection exists between osteogenesis and autophagy, an evolutionary conserved self-degradative system that delivers cytoplasmic constituents to the lysosomes 17 . In vivo, the deletion of FIP200, an essential component of the autophagosome complex, suppresses autophagy and causes osteopenia in mice by inhibiting osteoblast differentiation 18 . Similarly, knocking down autophagy-related gene ATG5, a component of the autophagosome, reduces bone mineralization 19 . In vitro, the autophagy proteins ATG7 and beclin 1 are required for mineralization in an osteoblastic cell line 19 . Therefore, we evaluated the activation and the role of autophagy in hMSC after inhibiting the expression of MagT1 or TRPM7 or culturing the cells under Mg deficient conditions.

Results
TRPM7 and MagT1 are overexpressed in hMSC induced to differentiate into osteoblasts. Confluent cells were cultured for 3, 6, 10 and 14 days in an osteogenic medium containing vitamin D (OM) or in their culture medium (CM) as a control. By real-time PCR we demonstrate an overexpression of TRPM7 and MagT1 in cells exposed to OM for 6 and 10 days from the beginning of the experiment (Fig. 1A). Western blot shows that both TRPM7 and MagT1 are upregulated in hMSC exposed to the osteogenic medium for 6 and 14 days (Figs 1B and S1A,B). It is noteworthy that while the expression of TRPM7 and MagT1 drops at day 14, the protein levels remain elevated until the end of the experiment. siRNAs against TRPM7 or MagT1 boost the expression of osteogenic differentiation markers. To investigate the role of TRPM7 and MagT1 in the osteogenic differentiation of hMSC, we transiently transfected the cells with specific siRNAs for TRPM7, MagT1 or with a non-silencing siRNA as a control (−) and then cultured hMSC in CM for 3 days. By real-time PCR MagT1 expression resulted completely suppressed and TRPM7 expression appeared dramatically reduced but not totally abrogated ( Fig. 2A). Western blot shows the downregulation of the two proteins (Figs 2B and S2A,B). To get an overview about Mg homeostasis, we measured total intracellular Mg 48 and 72 h after transfection without finding any significant difference between cells silencing TRPM7 or MagT1 and relative controls (Fig. 2C).
In siRNA transfected cells, we analyzed the expression of RUNX2 and COL1A1 and found it slightly upregulated even in the absence of the osteogenic cocktail (Fig. 3A). Moreover, cells downregulating TRPM7 or MagT1 maintained their sensitivity to the stimulatory effect of the osteogenic cocktail and upregulated both RUNX2 and COL1A1 more than controls (Fig. 3A). By ELISA, we demonstrate the significant increase of RUNX2 in hMSC After exposure to siRNA, hMSC were cultured in CM for 3 days. Real-Time PCR was performed on RNA extracted from hMSC using primers designed on the sequence of TRPM7 and MagT1. The controls, indicated as -, were exposed to non-silencing, scrambled sequences. (B) Western blot was performed on extracts from hMSC after 3 days silencing. Antibodies against TRPM7 or MagT1 were used. Actin was used as a control of loading. A representative blot is shown and quantification is provided in the Supplementary information (Fig. S2A). (C) Total Mg was measured using the fluorescent chemosensor DCHQ5 as described. Real-Time PCR was performed on RNA extracted from hMSC using primers designed on RUNX2 and COL1A1 sequence. (B) ELISA for RUNX2 and collagen type 1 was conducted on extracts from hMSC cultured in CM or OM for 5 days. The controls, indicated as -, were exposed to non-silencing, scrambled sequences.
downregulating TRPM7 or MagT1 after 5 days of osteogenic induction (Fig. 3B), while collagen type 1 is not induced probably because its accumulation represents a late event in osteoblastogenesis 15 . Osteogenic differentiation of hMSC ultimately leads to the deposition of calcium in the extracellular matrix. We therefore evaluated calcium deposition by Alizarin Red S staining 16 in silenced cells cultured in OM or CM for 14 days. In support to the results obtained by RT-PCR and ELISA, we detected some calcium deposits in siRNA transfected cells cultured in CM, but not in controls transfected with non silencing siRNAs (Fig. 4). Upon exposure to OM, siRNA transfected hMSC deposited much higher amounts of calcium phosphate crystals than non silenced cells.
Autophagy is involved in accelerating osteogenesis in hMSC exposed to siRNAs against TRPM7 or MagT1. Autophagy contributes to the osteogenic differentiation of hMSC 20 . In agreement with these findings, we show the conversion of microtubule-associated protein 1A/1B light-chain phosphatidylethanolamine conjugate (LC3B) to autophagosome-associated LC3B-II, which is the most widely used autophagosome marker 21 , in hMSC treated with siRNAs targeting MagT1 or TRPM7 for 3 days (Figs 5A and S5A,B). We also evaluated the total amounts of beclin 1, which contributes to the initiation of autophagosome formation by interacting with phosphatidylinositol 3-kinase 22 . Figures 5A and S5A,B show that beclin 1 is markedly induced in cells silencing TRPM7 or MagT1.
To reinforce these results, we performed a Tandem fluorescent-tagged LC3 assay to assess autolysosome function. hMSC were transfected with the plasmid containing the sequence for the fusion protein LC3-GFP-RFP. Briefly, in the lysosome where the pH is low, the fluorescence of GFP is quenched, while that of RFP is stable. Formation of autophagosomes increases the number of GFP-positive/RFP-positive (yellow) vesicles, which become GFP-negative/RFP-positive (red) once fusion with lysosomes occurs. Figure 5B confirms the induction of autophagy in cells silencing TRPM7 or MagT1 as indicated by the increase of the number of vesicles and of autophagolysosomes in silenced cells vs controls.
Considering the complex role of free calcium in autophagy 23 , it is noteworthy that we did not detect any modulation of intracellular free Ca in silenced cells vs their controls (Fig. 5C).
Next we tested whether bafilomycin A1, which inhibits autophagy by preventing the acidification of endosomes and lysosomes, affects the expression of RUNX2 in hMSC exposed to siRNA targeting MagT1 or TRPM7 for 3 days. Figure 5D shows that bafilomycin A1 prevented the increase of RUNX2 expression in siRNA treated

Low extracellular Mg enhances osteogenic differentiation of hMSC by activating autophagy.
We asked whether Mg deprivation has an effect on hMSC differentiation and found that culture in 0.1 mM Mg for 14 days enhances Ca deposition in the extracellular matrix as detected by Alizarin Red S staining (Fig. 6A). Accordingly, the expression of RUNX2 is higher in Mg deficient cells versus their controls both in the presence and in the absence of osteogenic stimuli (Fig. 6B).
No differences were observed in the content of total intracellular Mg in hMSC maintained in Mg deficient medium vs controls (Fig. 6C). Moreover, western blot shows that TRPM7 is upregulated after 3 days and MagT1 after 6 days of culture in medium containing 0.1 mM Mg (Figs 6D and S6A,B).
Next, we demonstrate that culture in low extracellular Mg (0.1 mM Mg) stimulates the conversion of LC3B to LC3B-II and upregulates beclin 1 in our experimental model (Figs 7A and S7A,B). These results were confirmed by Tandem fluorescent-tagged LC3 assay described above which shows an increase of the number of vesicles and of autophagolysosomes in Mg deficient hMSC (Fig. 7B). Figure 7C demonstrates no variation of intracellular free Ca between cells cultured in 0.1 or 1.0 mM Mg medium.
The contribution of autophagy to osteogenic differentiation is supported by the fact that bafilomycin A1 reduced the expression of RUNX2 in cells cultured in low (0.1 mM) or normal (1.0 mM) extracellular Mg for 3 days (Fig. 6B).
To the best of our knowledge this is the first evidence of a link between Mg, its transporters and autophagy.

Discussion
Mg is required for every biological process not only because it is necessary for the function of hundreds of enzymes but also because it maintains the active conformation of macromolecules, regulates second messengers, various transporters and ion channels 12 . Therefore, it is not surprising that the amounts of intracellular Mg are balanced by a coordinated interplay among channels and transporters mediating Mg influx, exchangers regulating its efflux, and Mg shuffling from organelles to cytosol and viceversa 24 . About 60% of total Mg is stored in the bone and a tight control of magnesium homeostasis is crucial for bone health 13 . A key event in bone formation is the differentiation of MSC into osteoblasts, a process which involves Mg and its transporters [5][6][7]11,25 . It has recently been reported that TRPM7, which is important in skeletogenesis 26 , acts as a mechanotransducer in murine MSC and commits their fate towards osteogenic differentiation in response to mechanical stimulation 5 . Also MagT1 11 and the Na/Mg exchanger SLC41A 27 contribute to osteoblastogenesis 28 . We focused our studies on TRPM7 and MagT1, both contributing to Mg transport into the cells 12 , in a model of hMSC induced to differentiate into osteoblasts. We show the overexpression of TRPM7 and MagT1 with a peak after 6 days of osteogenic induction and a gradual return to control levels within 14 days. The increase of the transcripts is paralleled by the upregulation of TRPM7 and MagT1 at the protein level, which is retained up to day 14 from osteogenic induction. We hypothesize that post-translational mechanisms might be implicated in maintaining high the amounts of TRPM7 and MagT1 until hMSC reach full differentiation. While further studies are required, it is noteworthy that our results with MagT1 are in agreement with a report showing the increase of MagT1 in rat MSC exposed to an osteogenic medium 11 .
To unravel the function of TRPM7 and MagT1 in hMSC, we utilized specific siRNAs and demonstrate that downregulating TRPM7 or MagT1 accelerates hMSC osteogenic differentiation. Our observation in hMSC silencing MagT1 is in disagreement with previous results in rat MSC showing that knocking down MagT1 inhibits osteogenic differentiation 11 . This discordance could be ascribed either to the different behavior of cells of different species or to the different protocols used to induce differentiation. While we used vitamin D to induce osteogenic differentiation, Zheng et al. used dexamethasone which has been shown to modulate TRPM7 in some tissues 29 .
Since i) MagT1 and TRPM7 are upregulated in hMSC differentiation, and ii) siRNAs targeting TRPM7 or MagT1 hasten the expression of osteogenic markers as well as Ca deposition, we hypothesize that these transporters contribute to coordinate hMSC response to osteogenic stimuli by preventing excessive osteoblastogenesis. In this light, TRPM7 and MagT1 might be considered as sensors-controllers of the extent of hMSC osteogenic differentiation.
Silencing TRPM7 in different cell types, among which colon carcinoma cells, thymocytes and human endothelial cells, does not influence intracellular Mg content [30][31][32] , while knocking down MagT1 in mammalian cell lines lowered the levels of intracellular Mg 33 . We did not detect differences in intracellular Mg content in hMSC silencing TRPM7 or MagT1, suggesting that either TRPM7 or MagT1 is sufficient to maintain intracellular Mg homeostasis in hMSC. Considering that many different Mg transporters have been described and account for Mg balance 24 , it is feasible to propose that also other proteins contribute to maintain Mg intracellular concentrations. A connection between bone health and autophagy has been described [17][18][19] . It is noteworthy that autophagy is activated in the early phases of hMSC differentiation 20 . It is also interesting that resting quiescent hMSC accumulate autophagosomes 34 , which indicates that the cells are ready to react to different stimuli by activating autophagy. Moreover, autophagy is induced in embryonic stem cells early during differentiation 35 and in somatic cells reprogrammed to induced pluripotent stem cells 36 . We suggest that siRNA targeting TRPM7 or MagT1 boosts the expression of markers of osteogenic differentiation, at least in part, by activating autophagy, which removes unnecessary organelles, generates substrates to supply energy and recycles macromolecular blocks to drive cell differentiation. Accordingly, bafilomycin A1, an inhibitor of autophagy, prevents the acceleration of osteogenic differentiation in our experimental model. Ca does not seem to be implicated in triggering autophagy in cells silencing TRPM7 or MagT1 as well as in hMSC cultured in low extracellular Mg. To this purpose, it is noteworthy that early after exposure to a Mg deficient medium, TRPM7 increases, while later, when TRPM7 declines to baseline levels, MagT1 is upregulated. We hypothesize that the sequential upregulation of the two proteins allows to maintain unaltered the concentrations of intracellular Mg. Moreover, Mg deficiency mimics the effects of silencing TRPM7 or MagT1 because it accelerates the osteogenic differentiation of hMSC 25 and this happens in association with the trigger of autophagy without any detectable increase of free Ca.
Apart from transporting Mg, TRPM7 and MagT1 serve other functions. MagT1 contributes to the oligosaccharyltransferase complex, thus being involved in the glycosylation of various proteins 37 . It should be noted that the components of the extracellular matrix are glycosylated and this is important for bone formation and remodeling 38 . Moreover, it has been proposed that MagT1 might regulate Mg transport by glycosylating Mg transporters 24 . TRPM7 possesses a kinase domain and we cannot rule out the possibility that silencing TRPM7 might affect the phosphorylation of specific substrates, some of which have been identified 24 . Of interest, one of the protein phosphorylated by TRPM7 is annexin 1, a Ca-regulated phospholipid binding protein implicated in the regulation of cell growth, apoptosis 39,40 and also in osteogenic differentiation 41 . On these bases, downregulating TRPM7 and/or MagT1 might impact on the post-translational modification of proteins.
In conclusion, we demonstrate that MagT1 and TRPM7 as well as Mg deficiency contribute to the regulation of hMSC osteogenic differentiation partly by modulating autophagy in a Ca-independent fashion.

Methods
Culture of hMSC. hMSC were isolated from adult human bone marrow withdrawn from bilateral punctures of the posterior iliac crests of healthy male volunteers, after obtaining informed consent from all the subjects at the Policlinico in Milano, in compliance with the Helsinki declaration, according to institutional guidelines and regulations of the Ethical Committee of "IRCCS Policlinico" Milano. These cells were used in previous studies 16,25 . The cells were cultured at 37 °C and 5% CO 2 in Dulbecco's Modified Eagle's Medium containing 10% fetal bovine serum and 2 mM glutamine (culture medium, CM). All the reagents for cell culture were from Sigma-Aldrich. The cells were used between passage 2 and 6.
To obtain a transient downregulation of MagT1 and TRPM7, we utilized the stealth siRNAs developed by Qiagen for TRPM7 and Invitrogen for MagT1. siRNAs targeting TRPM7 were transfected using HiPerFect Transfection Reagent (Qiagen) while for siRNAs targeting MagT1 we used Lipofectamine RNAiMAX (Thermo Fisher Scientific). Non-silencing, scrambled sequences were used as controls (−) 42 . Viable cells were counted using a cell counter (Logos Biosystems).
In some experiments, hMSC were cultured in Mg-free MEM (Invitrogen, Thermo Fisher Scientific) or Mg-free MEM supplemented with MgSO 4 (Sigma-Aldrich) to reach the physiological concentration, i.e. 1 mM.
To induce osteogenic differentiation, hMSC were seeded in 6-well or 96-well plates. Once the cells were confluent, an osteogenic induction cocktail was added to the medium (osteogenic medium, OM). The osteogenic cocktail contains 2 × 10 −8 M 1α,25-Dihydroxyvitamin D3, 10 mM β-glycerolphosphate and 0.05 mM ascorbic acid (Sigma-Aldrich) 16 . To analyze Ca deposition by hMSC, the cells were rinsed with PBS, fixed (70% ethanol, 1 h) and stained for 10 min with 2% Alizarin Red S (pH 4.2, Sigma-Aldrich) 43 . Alizarin Red S staining was released from the cell matrix by incubation in 10% cetylpyridinium chloride in 10 mM sodium phosphate (pH 7.0), for 15 min and the absorbance measured at 550 nm. The experiment was repeated three times in triplicate. Photographs were taken at 10X magnification.
Real-Time PCR. Total RNA was extracted by the PureLink RNA Mini kit (Thermo Fisher Scientific).
Single-stranded cDNA was synthesized from 0.2 µg RNA in a 20 µL final volume using High Capacity cDNA Reverse Transcription Kit, with RNase inhibitor (Thermo Fisher Scientific) according to the manufacturer's instructions. Real-time PCR was performed using the 7500 FAST Real Time PCR System instrument using TaqMan Gene Expression Assays (Life Technologies, Thermo Fisher Scientific): Hs00231692_m1 (RUNX2), Hs00164004_m1 (COL1A1), Hs00918928_g1 (TRPM7), Hs00997540_m1 (MagT1).
The housekeeping gene GAPDH (Hs99999905_m1) was used as an internal reference gene. Relative changes in gene expression were analyzed by the 2 −ΔΔCt method 16 . The experiments were repeated three times in triplicate.

Western blot analysis.
Western blot was performed on hMSC induced to differentiate into osteoblasts, exposed to siRNAs targeting TRPM7 and/or MagT1 or cultured in medium containing 0.1 mM Mg. After lysis, samples (80 µg/lane) were separated on SDS-polyacrylamide gel, transferred to nitrocellulose sheets at 150 mA for 16 h, and probed with antibodies against TRPM7 (Bethyl), MagT1 (Abcam), LC3B, beclin 1 (Cell Signalling Technology) and actin (Santa Cruz Biotechnology). Secondary antibodies were labelled with horseradish peroxidase (GE healthcare). The ECL Western Blotting Substrate (Thermo Fisher Scientific) was used to detect immunoreactive proteins. The western blots were repeated at least three times and a representative blot is shown. Densitometry was performed using ImageJ software and results are shown as the mean ± standard deviation.
Tandem fluorescent-tagged LC3 assay. The Tandem fluorescent-tagged LC3 assay, which is based on the different pH stability of two fluorescent proteins, allows to monitor autophagic flux and provides information about the number of vesicles and/or the progression to the late phase of autophagy 44 . Briefly, hMSC were transfected with a plasmid containing the sequence coding for the LC3 protein fused with two fluorophores, specifically RFP (red) and GFP (green) using lipofectamine 2000 for 4 h. Twenty-four hours post transfection hMSC were silenced for TRPM7 or MagT1 as described above. 48 h later the cells were fixed and analysed by confocal microscopy (LEICA SP8, magnification 40x). The early phase, characterized by phagosome formation, is detectable through the visualization of vesicles containing the two fluorophores (yellow fluorescence). The late phase, characterized by the fusion of the phagosome with the lysosome and the formation of the phagolysosome, is characterized by the cleavage of the GFP fragment, which is sensitive to the acidic pH of the lysosome, so that only RFP signal is detected (red fluorescence).

Quantification of intracellular Mg and Ca.
Total Mg content was measured on sonicated hMSC using the fluorescent chemosensor DCHQ5 (kindly donated by Prof. S. Iotti, Università di Bologna) as described 31,32 . Fluorescence intensities were acquired at 510 nm. Mg concentrations of the samples were obtained by the interpolation of their fluorescence with the standard curve performed using MgSO 4 .
To quantify free Ca, Fura-2/AM (10 μM) was added to the culture medium for 60 min. Then the cells were washed with a buffer containing NaCl 125 mM, KCl 5 mM, MgSO4 1.2 mM, CaCl 2 2 mM, glucose 6 mM, Hepes-NaOH buffer 25 mM (pH 7.4), removed by trypsinization, and suspended in the aforementioned buffer. Fluorescence intensities were acquired using a spectrophotometer with excitation wavelengths of 360 nm and emission at 450 nm 45 . Results were normalized on cell number.
ELISA. hMSC were exposed to CM or OM for 5 days. For the quantitative determination of RUNX2 and collagen type 1, Cusabio ELISA kit were used according to the manufacturer's instructions. ELISAs were performed at least three times, and each sample was measured in triplicate. Data are shown as the mean ± standard deviation. Statistical analysis. Statistical significance was determined using Student's t test and set as following: *P < 0.05, **P < 0.01, ***P < 0.001.