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A rapid and simple color test for detection of salicylate in whole hemolyzed blood.

Acetylsalicylic acid (ASA) is still one of the most commonly used therapeutic agents. Salicylic acid, the major metabolite of ASA, can be detected easily in urine using simple chemical spot tests such as ferric chloride or Trinder's reagent. In forensic cases, urine is often not available and the rapid detection of salicylate in whole hemolyzed blood can be difficult. This report describes the rapid and simple detection of salicylate using ferric chloride and a methanolic extract of whole blood. The color test is rapid and can detect salicylate at mid-therapeutic concentrations of 5 mg/dL. As little as 300 microL of whole blood is required and no equipment is needed. The color test can also be used with serum or plasma.