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Changes in the liver mitochondrial proteome after ischemia/reperfusion

Ischemia/reperfusion (I/R) injury of the liver is an important complication of liver surgery and transplantation. Mitochondrial function is central to this injury. In order to examine mitochondrial function, we assessed the hepatic mitochondrial proteome during I/R. C57Bl/6 mice were subjected to sham surgery or 90 min of partial hepatic ischemia and 1 hr of reperfusion. Liver mitochondria were isolated and separated by 2‐D PAGE with a pH range of 4–7. Image analysis was performed to assess protein expression between sham and I/R groups. 234 protein spots were increased or decreased in expression more than 2‐fold compared to mitochondria from sham mice. From these, 29 proteins were selected for identification. Protein spots were digested with trypsin analyzed by mass spectroscopy and identified using the Mascot database. Increased mitochondrial expression of hydroxyacyl‐coenzyme A dehydrogenase, methionine adenosyltransferase Iα, two heat shock protein 70‐related proteins, Es31 protein, transmembrane protein 4, glutamate dehydrogenase 1, sorbitol dehydrogenase precursor, fumarylacetoacetase, and peroxiredoxin‐6 was found. One protein had decreased expression; ATP5b protein. Our data demonstrate changes in mitochondrial proteins after I/R and are the first to identify mitochondrial expression of peroxiredoxin‐6.