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[Determination of amentoflavone in 11 species of Selaginella medicinal material by HPLC].

OBJECTIVE
To determine the content of amentoflavone in 11 species of Selaginella medicinal materials.


METHODS
HPLC was used with kromasil (250 x 4. 6 mm, 5 microm) column. The mobile phase consisted of methanol and phosphate buffer. The gradient condition was 0-->28 min with methanol 50%-->58%, and 30-->35 min with methanol 58%-->70%. The column temperature was 40 degrees C. The flow rate was 1. 0 ml/min and the detection wavelength at 330 nm.


RESULTS
Amentoflavone showed a good linearity in the range of 153 - 767 ng. The average recovery was 97.03% and RSD was 0. 80% (n = 5).


CONCLUSION
The method was rapid, accurate and can be used for quality control of Selaginella medicinal materials.