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Production of concentrated Streptococcus salivarius subsp. thermophilus by coupling continuous fermentation and ultrafiltration

Summary Continuous production of concentrated cells of Streptococcus salivarius subsp. thermophilus was achieved in a continuous stirred tank reactor coupled with an ultrafiltration module. A cellular productivity nine times higher than that obtained by conventional methods was observed. The viability of the cells produced was as satisfactory as that obtained by classical culture, but an increase in latency time and a slight decrease in acidification rate during downstream tests in milk were observed. Specific lactic acid productivity decreased as biomass increased. This process produced concentrated starters and lactic acid which could be purified by a downstream processing such as electrodialysis.