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Quantitative determination of a Mesalazine using reversed phase high- performance liquid chromatography (RP-HPLC)

Mesalazine was quantified in a fast, accurate and sensitive way using reversed-phase high-performance liquid chromatography (RP-HPLC) method. In this method, a separation column of porous silica particles L10 (300 × 4.6mm) was used, the flow rate is 0.8 ml/min, the column temperature is 25 °C, the reagent wavelength is 230 nm, and the injection volume is 20 μl. As for the mobile phase, it consisted of acetonitrile 10%  0.1 and phisphoric acid 0.1% and 90% water and the pH was 4. The method was validated by calculating the recovery that ranged between (95.0457-102.280%), and the correlation coefficient (0.9991) and linearity (20-160 μg/ml).The method was successfully applied for the quantitative determination of the mentioned drug.