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Effect of He-Ne laser irradiation on erythrocyte and lymphocyte membranes of children in vitro as studied by the intrinsic and extrinsic fluorescence techniques

In recent years the treatment of blood with low intensity laser irradiation has become popular in a variety of clinical applications due to its anti-inflammatory, biostimulative and immune-stimulatory effects etc. Despite of wide using of laser blood irradiation in the pediatric practice there is lack of information concerning the sensitivity of children blood cells to laser irradiation. At present study the influence of the He-Ne laser irradiation on the lipid physico-chemical state in lymphocytes and isolated erythrocyte membranes of 8-16 years old children using lipophilic fluorescence probe pyrene was investigated in vitro. It was shown that fluorescence parameters of pyrene incorporated into erythrocyte and lymphocyte membranes after laser irradiation ((lambda) equals 630nm) at dose of 24 J/cm2 at t equals 18 +/- 2 (degree)C were unchanged. The intensity of intrinsic protein UV-fluorescence ((lambda)ex equals 297 nm, (lambda)em equals 332 nm) of lymphocytes exposed to the same irradiation was decreased insignificantly. The obtained data indicate that He-Ne laser irradiation at the above dose does not affect the lipid microviscosity of erythrocyte and lymphocyte membranes.