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Phytochemical Screening and Acute Toxicity Evaluation of Leaves Extract of Two Fabaceae’s Species: Sesbania pachycarpa DC. and Indigofera berhautiana Gillett

Objective: The aim of this study is to screen chemical composition and to evaluate the acute general toxicity of leaves aqueous extracts from Sesbania pachycarpa DC. (Fabaceae), used in traditional medicine in Burkina Faso to treat malaria, helminthiasis, and aqueous extracts of the leaves from Indigofera berhautiana Gillet (Fabaceae) used to treat hepatitis and typhoid fever. Methods: Aqueous decoction of leaves from Sesbania pachycarpa DC. or Indigofera berhautiana Gillett which are the form of use recommended by traditional health practitioners, were used for tests. Aqueous extracts used to evaluate the acute toxicity, were studied on mice of strain NMRI. For the toxicity study, the doses of the aqueous extracts were respectively 5 mg/kg, 50 mg/kg, 300 mg/kg and 2000 mg/kg of body weight. Phytochemical screening was done to identify the phytochemicals contained in extracts. Results: Aqueous extracts of two species: Sesbania pachycarpa DC., Indigofera berhautiana Gillet were not toxic at the maximum dose of 2000 mg/kg of body weight. Phytochemical screening, showed coumarins, flavonoids, tannins, polyphenols compounds, steroids and/or triterpenes and saponosides in the aqueous extracts of the two species. Alkaloids were identified in Sesbania pachycarpa aqueous extract.


Introduction
Medicinal plants are widely used in Burkina Faso to treat the most diverse diseases possible. However, very few toxicological studies are conducted around these plants. The most vulnerable people who are sick, and among them children, pregnant women, are exposed to all kinds of plants that are recommended by traditional healers. It is necessary to carry out scientific studies on the plants used, in order to evaluate their toxicity.
During our ethnobotanical studies in Ouagadougou (Burkina Faso), leaves of Indigofera berhautiana Gillett are used internally to treat jaundice and typhoid fever.
According to Nacoulma [1], different parts of Sesbania pachycarpa DC., are used for therapeutic purposes. In internal use, the leaves are used to treat malaria, helminthiasis (nematodes), febrile body aches, gastritis. In external use, the leaves are used for the following cases: wounds, varicose ulcers, ear infections, dracunculiasis.
The antipicultural and toxic activity of Sesbania pachycarpa seeds has been demonstrated by Fafioye [2].
The biological activities of Sesbania pachycarpa have been described by several authors: antioxidant activity described by Cook et al. [3], Atawodi [4]. Ouattara et al. [5], antibacterial in the preventive treatment of syphilis, bacterial infection caused by Treponema pallidum, described by Berhaut [6], anti-malaria activity, antihypertensive, antibacterial for example against coughs, described by Nadembega et al. [7]. The increase in the use of medicinal plants as therapeutic agents is accompanied by an increased demand for evidence of the safety, efficacy and quality of products. The objectives of this study are to assess the acute toxicity of leaves aqueous extract of Sesbania pachycarpa and Indigofera berhautiana which are medicinal plants widely used in the traditional health system in Burkina Faso.

Plant Material
The studies were conducted at Ouagadougou's University Joseph KI-ZERBO, After harvest, the samples are dried, protected from light, at room temperature.

Decoction
The dried leaves are pulverized and sieved. We proceeded with the extraction by Journal of Biosciences and Medicines 500 ml of water for one hour, then the mixture was filtered on Wattman paper after cooling. The decoction is lyophilized and kept in a box, for studies.

Assessment of Acute General Toxicity
The method is that described by Done [8], Lompo et al. [9]. Female NMRI strain mice, 8 to 10 weeks old, weighing between 25 and 35 g were used for the tests.

Statistical Analyzes
All experiments are performed in triplicate and the results are expressed in means ± standard deviation using Microsft excel 2013.

General Acute Toxicity for Sesbania pachycarpa DC.
The results of the toxicity tests for Sesbania pachycarpa DC. are shown in Table   1. The results indicate that there were no dead animals in any lot of mice up to a dose of 2000 mg/kg. The mortality rate is therefore 0%. For body weight, controls were increased from 35 g to 36 g, mice receiving 2000 mg/kg aqueous extracts of Sesbania pachycarpa leaves increased from 34 to 36 g in 3 days. This result indicates that the aqueous extracts of Sesbania pachycarpa were not toxic. In the literature, toxicity was observed for Sesbania pachycarpa seeds, studied on fish [2]. This work has even contributed to the search for toxicity in the aqueous extracts of Sesbania pachycarpa leaves used by our populations. At the end of our work, we found no toxicity on mice, with aqueous extracts of leaves.

Acute General Toxicity for Indigofera berhautiana Gillett
The results of toxicity tests for Indigofera berhautiana are shown in Table 2 for the two batches of mice: controls and 2000 mg/kg.
There were no dead animals, which means that the mortality is 0% at the Journal of Biosciences and Medicines

Phytochemical Studies
The phytochemicals identified by the simple characterization tests are shown in Table 3. Apart from the alkaloids that we did not find in the aqueous extracts of Indigofera berhautiana leaves, both species contain small amounts of flavonoids, but rather tannins and polyphenols, steroids and triterpenes, saponosides.
The phytochemicals observed may be responsible for the biological activity of the extracts. Several studies have shown the antimicrobial activity of these phytochemicals [5] [12] [13] [14].
The identified compounds may be supplemented by subsequent HPLC analyzes, as well as by the determination of the active ingredients responsible for the

Conclusions
We evaluated the acute general toxicity of two plants from Fabaceae's family: Sesbania parchycarpa DC. and Indigofera berhautiana Gillett, commonly used in traditional medicine in Burkina Faso.
According to our studies, leaves aqueous extracts from Sesbania parchycarpa DC. or Indigofera berhautiana Gillett, which are recommended for use by patients, were not toxic in NMRI strain mice, at the dose of 2000 mg/kg of body weight.