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X-Ray Microanalysis and Image Mapping of Elements from Eggshells of Freshly Laid Eggs of Green Sea Turtles Chelonia mydas

Green turtle Chelonia mydas eggs were taken at oviposition for X-ray microanalysis and image. Untreated eggshells were analyzed for elemental composition and image mapping with an Oxford energy dispersive x-ray spectrometer (EDS) operating at 20kV and a working distance (WD) of 20mm. This involved qualitative, quantitative and area analysis. Qualitative analysis of characteristic X-rays (elements) distribution was displayed as a spectrum. SEM Quant (Oxford Instruments Ltd.) was used for quantitative analysis with ZAF internal standardardization (5 iterations), all elements and element composition expressed as weight percent. Representative sites were chosen for area analysis or image mapping of element distribution.