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Transcriptional Regulation of the Ovine Follicle-Stimulating Hormone-β Gene by Activin and Gonadotropin-Releasing Hormone (GnRH): Involvement of Two Proximal Activator Protein-1 Sites for GnRH Stimulation.

Previous studies from our laboratory demonstrated that a transgene consisting of the promoter for the ovine FSH β-subunit gene and a luciferase reporter (wt-oFSHβLuc) was expressed and regulated like the FSHβ gene in vivo and in vitro. In the present study pituitary cultures were prepared from these transgenic mice as well as mice carrying mutated oFSHβLuc lacking two functional activator protein-1 (AP-1) sites at −120 and −83 bp (mut-oFSHβLuc). These AP-1 sites were reported necessary for induction of oFSHβLuc by GnRH in a HeLa cell system. To examine the importance of the two AP-1 sites in mediating GnRH and activin effects in primary gonadotropes, pituitary cultures derived from transgenic mice were pretreated with follistatin to remove activin or activin-like factors present in the cultures. Follistatin lowered luciferase expression in cultures carrying both wt-oFSHβLuc and mut-oFSHβLuc transgenes by 74–86%, and subsequent addition of activin induced luciferase expression of both wt- and mut-transgene...