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Phytomolecules-Coated NiO Nanoparticles Synthesis Using Abutilon indicum Leaf Extract: Antioxidant, Antibacterial, and Anticancer Activities

Background NiO nanoparticles have attracted much attention due to their unique properties. They have been synthesized using chemical and physical techniques that often need toxic chemicals. These toxic chemicals cannot easily be removed from the nanoparticle’s surface, make them less biocompatible, and limit their biological applications. Instead, plants based green synthesis of nanoparticles uses phytomolecules as reducing and capping agents. These phytomolecules are biologically active with no or less toxic effects. Materials and Methods Phytomolecules-coated NiO nanoparticles were synthesized employing a green route using Abutilon indicum leaf extract. For comparative study, we also have synthesized NiO nanoparticles using the co-precipitation method. Synthesized nanoparticles were successfully characterized using different spectroscopic techniques. The synthesized nanoparticles were evaluated for antibacterial activity with agar well diffusion assay against different bacteria compared to standard drug and plant extract. They are also examined for anticancer potential using MTT assay against HeLa cancer cells, and further, their antioxidant potential was determined using DPPH assay. Biocompatibility of the synthesized nanoparticles was assessed against fibroblast cells. Results Phytomolecules-coated NiO nanoparticles were demonstrated superior antibacterial and anticancer performance against bacteria (E. coli, B. bronchiseptica, B. subtilis, and S. aureus) by presenting highest zone of inhibitions (18 ± 0.58 mm, 21 ± 0.45 mm, 22 ± 0.32 mm, and 23 ± 0.77 mm) and HeLa cancer cells by exhibiting the least cell viability percentage (51.74 ± 0.35%) compared to plant extract and chemically synthesized NiO nanoparticles but were comparable to standard antibiotic and anticancer drugs, respectively. Phytomolecules-coated NiO nanoparticles were also demonstrated excellent antioxidant activity (79.87 ± 0.43% DPPH inhibition) and biocompatibility (> 90% cell viability) with fibroblast cells. Conclusion Nanoparticle synthesis using the Abutilon indicum leaf extract is an efficient and economical method, produces biocompatible and more biologically active nanoparticles, which can be an excellent candidate for therapeutic applications.


Introduction
Currently, multidrug resistance (MDR) pathogenic bacteria have become a severe issue globally, and they are considered a significant cause of fatalities, about 700,000 deaths per year. 1 This number can be projected to 10 million deaths per year if the current use of antibiotics continues. 2 MDR bacterial infections are severely impacting healthcare and preventive practices. Thus, it is obligatory to find out new alternatives to combat these MDR pathogenic bacteria. [3][4][5] Besides, cancer is also one of the significant causes of death in human beings, and the burden of cancer patients has doubled from 1975 to 2000. 6 In 2018, over 18 million cancer cases and 9.6 million fatalities due to this disease were reported. 7 It is estimated that cancer cases will be more than 29.5 million by 2040. 8 Several advancements in molecular and cellular biology have taken place for treating the cancer disease through chemotherapy, but it also poses numerous side effects on cancer patients besides positive ones. 9 Therefore, it is essential to develop new anticancer drugs with improved efficacy, biocompatibility, and minimum side effects.
In this instance, metal and metal oxide nanoparticles have gained much attention. [10][11][12][13][14] They are considered most opportunistic and worthwhile because of their unique physical properties such as light-absorbing properties, large surface area to volume ratio, smaller size, surface plasmon resonance, etc. 9,15 With nanometer size, the nanoparticles can easily be penetrated the cell wall of the pathogenic MDR bacteria compared to conventional antibiotics, which is the most important and influential factor for their excellent antibacterial activity. Moreover, metal and metal oxide nanoparticles also have the capability to induce oxidative stress by generating reactive oxygen species (ROS) after penetrating cancerous cells, which leads the cells to demise. [16][17][18][19] Based on these properties, they have been extensively utilized for their biomedicine applications (antidiabetic, drug-delivery, antilarvicidal, anticancer, antioxidant, antibacterial, antifungal, etc.), biosensing (glucose, potassium ion, nitrite, etc.), photocatalysis, and memory storage devices. [20][21][22][23][24] However, NiO nanoparticles have attained great interest due to their unique properties (electron transfer ability, supper conductance, electrocatalysis, high chemical and thermal stability, antibacterial, antifungal, anti-inflammatory, etc.). NiO nanoparticles are environmentally active material as they have a wide bandgap (3.6-4.0 eV) and can be used for the adsorption and photocatalysis of heavy metals and synthetic dyes, respectively. [25][26][27] The nanoparticles are mostly synthesized using physical and chemical techniques; however, both these techniques are not easily scalable, demands more energy and hazardous chemical substances for reduction and capping. 12 The use of toxic chemicals for capping in these techniques compromises the nanoparticle's biocompatibility and limits their biomedical applications. 16 Consequently, the nanoparticle synthesis employing biological techniques, specifically plant-based, is considered an alternate, economical, fast, easily scalable, more biocompatible, and worthwhile approach over the chemical and physical techniques. 16,[28][29][30] Plant's based biological methods do not require toxic chemical substances as they used plant's phytomolecules (polyphenols, alkaloids, terpenoids, saponins, etc.) as reducing and capping agents for the nanoparticle's synthesis. 31 Different plants and their other parts, such as stem, leaves, flowers, seeds, shoots, etc., have been widely utilized to synthesize several kinds of nanoparticles. 9,15,16,20,21 Some of these plants are biologically active but have serious biocompatibility issues, and some are not biologically active. For example, many researchers have used Stevia leaf extract for different nanoparticle synthesis. But in 2019, leaves and crude extracts of this plant were included in an FDA import alert with concerns about their safety for use in foods or supplements and potential for toxicity. 32 Therefore, nanoparticles for biological applications must be needed to be synthesized with such plants that have no biocompatible issue, and they should also be biologically active. In this regard, Abutilon indicum has significant importance as this plant is biologically active and has no biocompatibility issues. Abutilon indicum is a medicinal plant and extensively utilized for the treatment of different types of diseases, including blood dysentery, allergy, bronchitis, pulmonary tuberculosis, mumps, rheumatism, diarrhea, vaginal infections, cleaning wounds and ulcers, relieving thirst, piles, jaundice, urinary disease, and leprosy since ancient times. 33 Abutilon indicum has numerous natural products, including flavonoids, phenolic acids, alkaloids, saponins, terpenoids, etc., that enable this plant to show different biological activities (analgesic, 34 antiinflammatory, 35 antidiabetic, 36 antioxidant, 37 antimicrobial, 38 anticancer, 39 hepatoprotective, 40 antiasthmatic, 41 antilarvicidal, 42 etc.). Herein, we have synthesized the phytomolecules-coated NiO nanoparticles using the leaves extract of Abutilon indicum first time as per the author's best knowledge. For comparative study, we also chemically synthesized the NiO nanoparticles with the coprecipitation method. The chemically synthesized and phytomolecules-coated NiO nanoparticles were further applied to evaluate their antioxidant, antibacterial, and anticancer activities.

Plant Material Collection and Extract Preparation
The fresh Abutilon indicum leaves were collected from a graveyard near old Anarkali, Lahore, Pakistan. The leaves of Abutilon indicum were washed with deionized (DI) water and shade dried for one month. The dried leaves were then ground into powder using a commercial grinder ( Figure 1). The plant leaves powder was stored in glass bottles for future use.
The 20 g of leaves powder was taken in a 500 mL beaker, and then methanol (120 mL) and DI water (180 mL) were added. Subsequently, the reaction mixture was heated at 50 °C for 60 min with continuous stirring. After, the reaction mixture was set aside overnight by covering the beaker with aluminum foil. The reaction mixture was then filtered with filter paper to remove the residues of the plant's leaves powder ( Figure 1). Finally, the obtained leaf extract was stored in a glass bottle and stored in the refrigerator at 4 °C for further use.

Synthesis of Phytomolecules-Coated NiO Nanoparticles
The leaf extract's 150 mL was taken in a 500 mL beaker, and subsequently, 3 g of Ni(NO 3 ) 2 .6H 2 O was added. After, the ethylene glycol (1.5 mL) was added into the reaction mixture and stirred continuously until complete mixing (20 min) at 30 °C ( Figure 1). In another beaker, 150 mL leaves extract was added, and subsequently, 3 g citric acid was then transferred in it and stirred for 60 min at 30 °C. After, both solutions were mixed, and the resultant reaction mixture was stirred continuously at 30 °C for 60 min to obtain the greenish color dispersion of NiO nanoparticles ( Figure 1). After the filtration with filter paper, the obtained phytomolecules-coated NiO nanoparticles were washed using DI H 2 O thrice times and dehydrated overnight at 80 °C and then calcinated at 500 °C for 120 min. Finally, the black color phytomolecules-coated NiO nanoparticles were obtained and transferred in a glass bottle, and stored for further use.

Synthesis of NiO Nanoparticles with Co-Precipitation Method
Ni(NO 3 ) 2 .6H 2 O and NaOH were used as a precursor for synthesizing the NiO nanoparticles with the coprecipitation method. In brief, a 0.1 M solution of Ni salts and a 0.8 M solution of NaOH was prepared separately in DI water. After the NaOH solution was added dropwise into the Ni salts solution within 30 min, the subsequent resultant solution was further stirred at 80-85 °C for 6 hrs. Finally, the green color precipitates were formed, washed with ethanol and DI water thrice times. The precipitates were placed in an oven at 120 °C for drying. The obtained NiO nanoparticles were further annealed at 550-600 °C for 6 hr, and finally, black colored chemically synthesized NiO nanoparticles were obtained. They were stored in an airtight container for further use.

Characterization X-Ray Diffraction Analysis
The crystallinity and purity of the green and chemically synthesized NiO nanoparticles in powder form were determined using the powder X-ray diffraction (XRD) (Bruker D2 PHASER with LYNXEYE XE-T detector, Haidian, Beijing, China) with a wavelength (λ) of 0.154 nm over the 2θ range 4-90°.

Scanning Electron Microscope (SEM) and Energy-Dispersive X-Ray (EDX) Spectroscopy
The synthesized nanoparticles' morphology was characterized using an SEM (Quattro S) by placing the dried powder sample on the carbon tape. The compositional analysis was carried out with an energy-dispersive X-ray (EDX) spectroscopy using Thermo Fisher Scientific Ultradry (Madison, WI, USA) attached with SEM.

Zetasizer Dynamic Light Scattering (DLS)
The green and chemically synthesized NiO nanoparticles were dissolved in DI water and sonicated for 5 min at 25-30 °C. About 10 mm sample solution was taken out and placed on a glass cuvette. After that, the cuvette was placed in the cell holder, and scanning was performed using a dynamic light scattering particle size analyzer (Malvern Zetasizer Nano ZS, Worcestershire, WR14 1XZ, UK) from 1 to 500 nm at 25-30 °C.

UV-Visible Spectrophotometric Analysis
The green and chemically synthesized NiO nanoparticles were dissolved in DI water and sonicated for 5 min at 25-30 °C. The nanoparticles solution and plant extract were then transferred in a quartz cuvette, and after being placed in the cell, the absorption maxima were determined from 200 to 800 nm using a UV-Visible spectrophotometer (Shimadzu 1700, Columbia, Maryland, USA) at 25-30 °C.

Fourier Transform Infrared (FTIR) Analysis
The dried powder of green synthesized NiO nanoparticles and plant extract was placed on the quartz slide. The FTIR spectrum was measured from 450 to 4000 using Perkin Elmer Spectrum 100 spectrophotometer (Bridgeport Avenue Shelton, CT 06484-4794, USA) at 25-30 °C.

Antibacterial Activity Evaluation
The antibacterial propensity of the phytomolecules-coated NiO nanoparticles was assessed against four bacterial strains (B. subtilis, S. aureus, B. bronchiseptica, and E. coli) compared to Leflox (antibiotic drug) and plant extract following the agar well diffusion method. 43 In a typical procedure, each sample solution of different concentrations such as 10 mg/mL, 20 mg/mL, 30 mg/ mL, and 40 mg/mL was prepared in DI water by sonicating for 10 min to ensure uniform dispersion. After, 35 mL nutrient agar was added in petri dishes (diameter × H = 90 mm × 15 mm, dish capacity ~90 mL), and subsequently, inoculum (3 mL) of each bacterial strain was transferred. The inoculum of each bacteria strain was prepared at 1 × 10 8 CFU/mL. The petri plates were then set aside for 15 to 20 min to solidify the molten nutrient agar. After, the four holes of 4 mm in diameter were created in each petri dish with a pasteurized hollow-iron rod. The holes were then filled with 50 µL of each sample solution (phytomolecules-coated NiO nanoparticles, plant extract, and standard drug) and kept on a flat surface for 1 hr. The petri dishes were then incubated at 37 °C for 24 hr. Each sample's antibacterial activity was then determined based on the appearance of zones of inhibitions (ZOIs) in the petri dish, and ZOIs were measured with the Vernier caliper.

Cytotoxic Activity
The anticancer activity of the phytomolecules-coated NiO nanoparticles compared to the standard anticancer drug (doxorubicin) and plant extract was determined with the MTT colorimetric method against the HeLa cancer cells (Sigma-Aldrich, Germany). 44 The Cell Proliferation Kit I (MTT) was used for cytotoxic analysis. In brief, HeLa melanoma cells were added into 96 microtiter well plate, and 150 µL DMEM was added, and the well plate was submit your manuscript | www.dovepress.com

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International Journal of Nanomedicine 2021:16 then incubated at 37 °C for 24 hr to obtain cell density 5 × 10 8 cells/well (cell's confluency was 75-80% measured by trypan blue-stained cells using a hemocytometer). Each sample solution (phytomolecules-coated NiO nanoparticles, standard drug, and plant extract) was prepared in DI water at different concentration levels (1,5,10,15,30,60, and 120 μg/mL). After, 10 µL of each sample solution was separately transferred to each well and again re-incubated for 24 hr at 37 °C. The cells were then centrifuged to remove supernatant and washed times thrice with phosphate buffer saline (PBS). The 100 µL of MTT reagent (0.6 mg/mL) was transferred to each well and again reincubated for 4 hr at 37 °C, and then DMSO (100 µL) was transferred to each well for solubilizing the undissolved formazan's crystals. After the plate was placed on the agitator for 15 min and then using the Varian Eclipse spectrophotometer, the absorption-maxima of formazans at 570 nm was measured. The cell cytotoxicity percentage was measured with the following equation; % Cell viability = (OD sample /OD control ) × 100, Where OD control is referred for the cancer cells without any treatment and only served with media. The OD sample is referred for the cancer cells treated with different samples' concentrations (phytomolecules-coated NiO nanoparticles, chemically synthesized NiO nanoparticles, an anticancer drug, and plant extract).

Live/Dead Staining Assay
We investigated the HeLa cancer cell viability with the fluorescent staining technique to further affirm the cytotoxicity using the live/dead double staining kit (viable cells stain with green and dead cells with red). The same experiment repeated as described above till cancer cells treated with different samples (10 µL of 120 μg/mL) and subsequent incubation. After, the staining solution (4 μg/ mL) was added to each well at 37 °C and incubated for 20 min. The photographs were taken with an Olympus BX53 fluorescence microscope (excitation wavelength 488/545 nm for viable/dead cells).

Antioxidant Activity in Terms of DPPH Percentage Inhibition Assay
The phytomolecules-coated NiO nanoparticles' antioxidant activity was determined by scavenging the DPPH free radical compared to plant extract, chemically synthesized NiO nanoparticles, and external standard butylated hydroxytoluene (BHT) via the protocol previously described by Khan et al. 16 In brief, DPPH solution of 0.1 mM and each sample's solution with different concentrations (25,50,75, and 100 µg/mL) was separately prepared in ethanol and DI water, respectively. Afterward, each sample's solution was individually mixed with a DPPH solution. The resultant reaction mixtures were stirred for 10 min at 30 °C and set aside for 24 hr. The antioxidant activity in terms of DPPH percentage inhibition was then determined using a UV-Visible spectrophotometer at 517 nm. The DPPH percentage inhibition was calculated by using the following equation: where, A s is the absorbance of the sample, and A c is the absorbance of the control (only DPPH solution).

Antioxidant Activity in Terms of Linoleic Acid (%) Inhibition
The antioxidant potential of phytomolecules-coated NiO nanoparticles in terms of linoleic acid (%) inhibition was determined in comparison to plant extracts and chemically synthesized NiO nanoparticles, following the protocol reported by Khan et al. 45 In detail, 100 µg/mL concentration of each sample was added to the solution mixture of 0.2 M sodium phosphate buffer (pH 7.0, 10 mL), 99.99% ethanol (10 mL), and linoleic acid (0.13 mL). The resulting solution's total volume was made up to 25 mL with DI water and subsequently incubated for 360 hrs at 40 °C. The extent of oxidation was measured using the thiocyanate method. Accordingly, 0.2 mL of the sample solution was taken and then added to 10 mL of ethanol (75%). Subsequently, 0.2 mL of aqueous ammonium thiocyanate solution (30%) and 0.2 mL FeCl 2 (20 mM in 3.5% HCl) was added. The reaction mixture was stirred for 3 min, and the absorption maxima were then measured at 500 nm wavelength. The percentage inhibition of linoleic acid was calculated using the following formula: % Inhibition = [100 − (Absorbance of sample)/(absorbance of control)] × 100 The Butylated hydroxytoluene was used as an external standard, and the control only consisted of linoleic acid without any treatment.

Biocompatibility Evaluation
The biocompatibility of the phytomolecules-coated NiO nanoparticles against the Human foreskin fibroblasts cells was evaluated compared to chemically synthesized NiO nanoparticles and plant extract via the standard protocol reported by Hoyo et al. 46 The fibroblasts cells were maintained in 4 ppm of DMEM (Dulbecco's Modified Eagle International Journal of Nanomedicine 2021:16 submit your manuscript | www.dovepress.com DovePress Medium) for 24 hr at 37 °C temperature in a humidified atmosphere with 5% CO 2 . The cells at pre-confluence were harvested in trypsin-EDTA (ATCC-30-2101) and then seeded on a 24-well microtiter plate at the cell's density of 1.0×10 5 cells per well for 24 hr at 37 °C. The cells were then washed using PBS. After the 50 µL of each sample's solution at 120 μg/mL were immersed in each well separately, and 1 mL of DMEM was added in each well. The 24-well microtiter plate was then incubated for 24 hr at 37 °C. The cells were then stained with AlamarBlue (100 µL, 10% v/v) at 37 °C for 4 hr. Finally, the cell viability was calculated by measuring the optical density (OD) at the absorbance of 570 nm using a microplate reader: Cell viability (%) = (OD value of treated cells)/(OD value of control) ×100

Live/Dead Staining Assay
To further affirm the biocompatibility, we investigated the fibroblasts cells viability with fluorescent staining technique using the live/dead double staining kit (viable cells stains with green and dead cells with red). The same experiment repeated as reported by 46 till fibroblasts cells were treated with different samples (10 µL of 120 μg/mL) and subsequent incubation. After, the staining solution (4 μg/mL) was added to each well at 37 °C and incubated for 20 min. The photographs were taken with an Olympus BX53 fluorescence microscope (excitation wavelength 488/545 nm for viable/dead cells).

Statistical Analysis
All the experiments (antibacterial, anticancer, antioxidant, and biocompatibility) were conducted three times, and the results are presented as mean ± standard deviation. One-way ANOVA at a fixed significance level (0.05) and Tukey's test were also applied to the results to determine the significance.

Characterization UV-Visible Analysis
The absorption maxima of plant leave extract, and phytomolecules-coated NiO nanoparticles were determined with a UV-Visible spectrophotometer. Figure 3A

FTIR Analysis
FTIR analysis was carried out to identify functional groups of phytomolecules responsible for synthesizing the phytomolecules-coated NiO nanoparticles by acting as reducing and capping agents. Figure 3C shows the FTIR spectra of   Figure 4A and Figure S1A show the XRD spectrum of the phytomolecules-coated NiO and chemically synthesized NiO nanoparticles.

SEM and Zeta Potential Studies
The morphologies of the phytomolecules-coated NiO nanoparticles and chemically synthesized NiO nanoparticles were determined using SEM. Figure 4B and Figure  S1B show the SEM images of the green synthesized and chemically synthesized NiO nanoparticles, respectively. SEM results demonstrate that they are appropriately dispersed and highly crystalline and have cubic (green synthesized) and spherical (chemically synthesized) DovePress morphology. 27,52 Some of the synthesized nanoparticles are found agglomerated. The average nanoparticle sizes of chemically synthesized and phytomolecules-coated NiO nanoparticles measured by Zetasizer dynamic light scattering system were 452.49 ± 0.6 nm ( Figure S1C) and 443.46 ± 0.9 nm (Figure 4C), respectively. The PDI value of green synthesized was observed 0.319, while for chemically synthesized, it was 0.215. The stability of the chemically synthesized NiO nanoparticles and phytomolecules-coated NiO nanoparticles was determined by measuring their zeta potential. The stability of the nanoparticles is associated with more negative charges. The results indicated that the phytomolecules-coated NiO nanoparticles have −10.95 mV zeta potential while chemically synthesized NiO nanoparticles have −18.50 mV zeta potential. Similar zeta potential results were reported by Kumar et al for the chemically synthesized, and green synthesized NiO nanoparticles. 54

EDX Analysis
The compositional analysis of the phytomolecules-coated NiO nanoparticles and chemically synthesized NiO nanoparticles was carried out with EDX, and Figure 4D and Figure S1D show the EDX pattern, respectively. The EDX peaks at 0.85 KeV and 7.48 KeV are attributed to the presence of "Ni". 27,49 While the peak at 0.52 KeV is indicated the presence of oxygen "O." Thus, the presence of nickel and oxygen peaks in the EDX spectrum validated the successful synthesis of phytomolecules-coated NiO nanoparticles. Besides, two sharp and intense EDX peaks at 0.27 KeV and 0.392 KeV are also evident in the EDX pattern, which corresponds to the presence of carbon "C" and nitrogen "N," respectively. 27,31 The carbon and nitrogen peaks might originate from the phytomolecules of plant's leaves extract adsorbed on the surface of phytomolecules-coated NiO nanoparticles.
On the other hand, sharp nickel and oxygen EDX peaks are evident in the EDX pattern of the chemically synthesized NiO nanoparticles. The small EDX peak associated with carbon is also apparent in the EDX pattern, which might be attributed to the carbon tape signal that holds the nanoparticles, as shown in Figure S1D. Hence, EDX pattern results were corroborated that the phytomoleculescoated NiO nanoparticles of our interest have been successfully fabricated using Abutilon indicum leaves extract.

Antibacterial Propensity
The antibacterial propensity of the phytomolecules-coated NiO nanoparticles was determined compared to the leaf extract of Abutilon indicum and antibacterial drug (Leflox). Figures 5A-D and 6A shows the antibacterial propensity results in the form of ZOIs. The superior antibacterial performance was demonstrated by the phytomolecules-coated NiO nanoparticles at the concentration of 40 mg/mL against Gram-negative (E. coli and B. bronchiseptica) and Gram-positive (B. subtilis and S. aureus) bacteria by demonstrating the highest ZOIs (18 ± 0.58 mm, 21 ± 0.45 mm, 22 ± 0.32 mm, and 23 ± 0.77 mm) respectively, compared to plant extract and chemically synthesized NiO nanoparticles at the same concentration level. The results also demonstrated that the antibacterial propensity of the phytomolecules-coated NiO nanoparticles was comparable to the standard antibiotic drug at all tested concentrations against the bacterial strains. It was interesting to note that leaves extract of Abutilon indicum also exhibited the inhibitory action against all the bacterial strains. This implies that the leaves extract has biologically active phytomolecules that have the ability to kill bacterial strains. 9,37 The nanoparticles, plant extract, and antibiotic drug were demonstrated the concentration-dependent antibacterial propensity.
Further, it was also observed that phytomoleculescoated NiO nanoparticles displayed excellent antibacterial performance against Gram-positive than Gram-negative bacterial strains. This might be due to the variances in the chemical structure of the cell wall of both the bacteria and further their different level of susceptibility towards metal oxide nanoparticles. 27 A similar more antibacterial effect of NiO nanoparticles towards Gram-positive than Gramnegative bacteria was also reported by previous studies. 27,55 Our phytomolecules-coated NiO nanoparticles displayed a more antibacterial effect compared to previously reported against S. aureus, B. subtilis, and E. coli. 27,56 This might be because of the adsorption of biologically active natural products of the Abutilon indicum leaves extract on NiO nanoparticles' surface that was also participated along with nanoparticles (synergetic) to inhibit bacteria's growth. The exact antibacterial mechanism of action of phytomolecules-coated NiO nanoparticles is still unclear. But many studies proposed that the phytomolecules-coated NiO nanoparticles in the solution release reactive radicals Ni 2+ ions that get attached to the negatively charged cell wall of bacteria due to electrostatic force of attraction ( Figure 6B). The strong adhesion of Ni 2+ ions to the bacterial cells disrupts the cell membrane, leading the bacterial cell to demise. 27

Anticancer Activity
The phytomolecules-coated NiO nanoparticles were evaluated for their anticancer potential against HeLa cancerous cell line compared to a plant extract and standard anticancer drug. Figure 7 shows the anticancer result in terms of cell viability percentage. Results of cell viability percentage were demonstrated that phytomolecules-coated NiO nanoparticles exhibited a significant cytotoxic effect on HeLa cancer cells compared to chemically synthesized NiO nanoparticles and leaves extract of Abutilon indicum. The cytotoxic effect of phytomolecules-coated NiO nanoparticles on HeLa cancer cells was comparable to standard anticancer drugs, and both presented more than 50% cytotoxicity. Moreover, results also showed that the plant's leaves extract exhibited good cytotoxicity, which revealed that it has such phytochemicals that are biologically active towards cancerous cells. Moreover, phytomolecules-coated NiO nanoparticles, plant extract, and anticancer drug were presented the concentration-dependent anticancer propensity against HeLa cancer cells, and the maximum cytotoxic effect was observed with their 120 µg/ mL concentration. [57][58][59] To observe the morphological changes in HeLa cancer cells after treatment with concentration 120 µg/mL of plant extract, green synthesized NiO nanoparticles, and chemically synthesized NiO nanoparticles, we employed an inverted microscope (Nikon Eclipse TE200) and the results are displayed in Figure 8A-D. Results show that HeLa cancer cells endured severe morphological alterations, including reducing the cytoplasm and cell rounding.
We further investigated the HeLa cancer cell viability with the fluorescent staining technique to affirm the cytotoxicity, and the results are presented in Figure 8E

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The remarkable anticancer propensity outcomes of the phytomolecules-coated NiO nanoparticles might be attributed to their physical properties (large surface area, morphology, and smaller particle size) and their surface's functionalization the biologically active phytochemicals of Abutilon indicum leaves extract. Reports demonstrate that Abutilon indicum leaves extract possesses molecular functionalities that have significant anticancer potential against different cancerous cells. 9,38 The enhanced anticancer results were reported with the green synthesized nanoparticles compared to nanoparticles prepared with other methods. 60,61 Hence, it can be concluded that nanoparticle's physical characteristics and functionalization with biologically active molecules have a significant role in enhancing the anticancer potential.

Antioxidant Activity in Terms of DPPH Percentage Inhibition
The antioxidant activity of the phytomolecules-coated NiO nanoparticles was evaluated by scavenging the DPPH free radicals. Results were indicated that all the tested samples displayed concentration-dependent antioxidant activity ( Figure 9). The phytomolecules-coated NiO nanoparticles were presented the superior DPPH scavenging ability compared to plant extract and chemically synthesized NiO nanoparticles but slightly lower than standard (BHT). On the other hand, chemically synthesized NiO nanoparticles were demonstrated the least antioxidant activity. It was noteworthy that plant extract also exhibited good antioxidant activity by scavenging the DPPH. These results indicated that Abutilon

Antioxidant Activity in Terms of Linoleic Acid Peroxidation Inhibition
The antioxidant activity of the phytomolecules-coated NiO nanoparticles was further evaluated in terms of linoleic acid peroxidation inhibition compared to plant leaves extract, chemically synthesized NiO nanoparticles and commercial standard (BHT). Results were displayed that  DovePress the phytomolecules-coated NiO nanoparticles presented the superior linoleic acid peroxidation inhibition percentage (30.05 ± 0.07%) compared to chemically synthesized NiO nanoparticles but slightly lower than plant extract and BHT ( Figure S2). On the other hand, chemically synthesized NiO nanoparticles were demonstrated the least antioxidant activity (50.50 ± 0.70%). These results indicated that Abutilon indicum leaves extract is a rich source of natural antioxidants. Moreover, the superior antioxidant activity of the phytomolecules-coated NiO nanoparticles compared to chemically synthesized NiO nanoparticles might be attributed to the presence of plant extract's phytomolecules (flavonoids, phenolic acids, alkaloids, etc.) on the surface of nanoparticles.

Biocompatibility Studies
The biocompatibility of the phytomolecules-coated NiO nanoparticles was evaluated in vitro on fibroblast cells compared to plant leaves extract and chemically synthesized NiO nanoparticles, and the results are presented in the form of cell viability (%) as shown in Figure 10A. The results were demonstrated that the least cell viability percentage of fibroblast cells (80.46 ± 0.31%) was observed with chemically synthesized NiO nanoparticles. On the other hand, phytomolecules-coated NiO nanoparticles were presented > 90% cell viability (%) with the fibroblast cells. Moreover, it was interesting to note that plant leaves extract has such phytochemicals that are biocompatible and exhibited excellent biocompatibility with the fibroblast cells.
To further confirm the biocompatibility, we investigated the fibroblast cells viability with the Live/dead fluorescent staining technique, and the results are displayed in Figure  10B-E. The results indicated that chemically synthesized NiO nanoparticles had pose more toxic effect on fibroblast cells as many dead cells (Red) appeared compared to plant extract and green synthesized NiO nanoparticles. The excellent biocompatibility of the phytomolecules-coated NiO nanoparticles might be attributed to the presence of different phytochemicals of plant's leaves extract on their surfaces. Henceforth, phytomolecules-coated NiO nanoparticles can be used for various biological applications. Both cell viability percentage results and staining assay results are found consistent with each other.

Conclusions
In this work, phytomolecules-coated NiO nanoparticles were successfully synthesized, employing a green route using Abutilon indicum leaf extract as reducing and capping agents. The phytomolecules-coated NiO nanoparticles were characterized by FTIR, UV-Visible, XRD, SEM, and EDX. The phytomolecules-coated NiO nanoparticles synthesized using plant's leaves extract showed excellent antibacterial performance by inhibiting the growth of Gram-negative and Gram-positive bacterial strains compared to plant extract and chemically The antioxidant activity results of phytomolecules-coated NiO nanoparticles in terms of %DPPH scavenging at different concentration levels than plant extract, chemically synthesized NiO nanoparticles, and BHT. Note: Tukey based heterogeneous lower-case letters represent significant pairs. submit your manuscript | www.dovepress.com

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International Journal of Nanomedicine 2021:16 synthesized NiO nanoparticles. However, they demonstrated comparable antibacterial activity compared to the standard antibiotic drug. The phytomolecules-coated NiO nanoparticles were further exhibited significant anticancer activity against the HeLa cancer cell lines, which was also comparable to the standard anticancer drug (Doxorubicin). The phytomolecules-coated NiO nanoparticles were also demonstrated excellent antioxidant activity and biocompatibility with fibroblast cells compared to chemically synthesized NiO nanoparticles. It was interesting to note that Abutilon indicum leaves extract was also found active towards antibacterial, antioxidant, and anticancer activities. Thus, antioxidant, antibacterial, and anticancer activities results displayed the potential of phytomoleculescoated NiO nanoparticles for their biomedical applications. Our results further recommended that phytomolecules-coated NiO nanoparticles exhibited excellent biological activities and biocompatibility due to the synergetic effect (physical properties and adsorbed phytomolecules on their surfaces). Hence, nanoparticles synthesis using leaf extract of Abutilon indicum is an efficient, robust, economical, and green method that produces biocompatible and biological active nanoparticles.