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Genetic Heterogeneity of the β-Globin Gene in Various Geographic Populations of Yunnan in Southwestern China

Objectives The aim of this study was to investigate the geographic distribution of β-globin gene mutations in different ethnic groups in Yunnan province. Methods From 2004 to 2014, 1,441 subjects with hemoglobin disorders, identified by PCR-reverse dot blot and DNA sequencing, were studied according to ethnicity and geographic origin. Haplotypes were examined among 41 unrelated thalassemia chromosomes. Results Eighteen β-thalassemia mutations and seven hemoglobin variants were identified for 1,616 alleles in 22 different ethnic groups from all 16 prefecture-level divisions of Yunnan. The prevalence of β-thalassemia was heterogeneous and regionally specific. CD 41-42 (-TCTT) was the most prevalent mutation in the populations of northeastern Yunnan. CD 17 (A>T) was the most common mutation in the populations of southeastern Yunnan, especially for the Zhuang minority, whereas Hb E (CD 26, G>A) was the most prevalent mutation in populations of southwestern Yunnan, especially for the Dai minority. Among the seven types of haplotypes identified, CD 17 (A>T) was mainly linked to haplotype VII (+ - - - - - +) and IVS-II-654 (C>T) was only linked to haplotype I (+ - - - - + +). Conclusion Our data underline the heterogeneity of β-globin gene mutations in Yunnan. This distribution of β-globin mutations in the geographic regions and ethnic populations provided a detailed ethnic basis and evolutionary view of humans in southern China, which will be beneficial for genetic counseling and prevention strategies.

Introduction β-thalassemia is one of the most common monogenic recessive diseases and it is the result of the presence of a mutation in one or two β-globin genes. It is characterized by decrease or absence of β-globin chain synthesis. β-thalassemia is prevalent in Mediterranean countries as well as in tropical Africa, southeast Asia and southern China. Approximately 800 different mutations of the β-globin gene have been documented in the HbVar database and more than 50 of them have been reported in China. The spectrum of β-globin gene mutations is variable within different ethnic groups. A specific racial group may have its own particular spectrum of mutations, reflecting significant genetic heterogeneity in the population. Characterizing the spectrum and frequency of β-globin gene mutations in different populations is useful for genetic counseling and prevention strategies.
The mutations responsible for β-thalassemia have been well documented in southern China, especially in Guangxi [1], Hongkong [2], Hainan [3] and Guangdong province [4]. However, mutations causing thalassemia have been studied less in the populations of Yunnan province. Yunnan province, a region located in southwestern China, has the Tropic of Cancer running through its southern part and has been known as the crucial gate of southeast Asia. The territory of Yunnan is largely tropical and consists of a subtropical plateau, especially in the southern and southwestern prefectures, and has the highest incidence of malaria in China [5]. It is characterized by a high incidence of hemoglobin variants [6]. Among the 56 recognized ethnic groups in China, twenty-six are found in Yunnan. The total population of Yunnan includes 46 million people and consists of many ethnic groups. Two-thirds are of the Han ethnic group (32 million), and the remaining population of 14 million consists of 25 different ethnic groups including Yi (11%), Hani (3.5%), Bai (3.4%), Dai (2.6%), Zhuang (2.6%) and others (national census in 2011).
Identification of β-globin gene mutations in the different regions will be helpful to explain the heterogeneity and prevalence of β-thalassemia in different ethnic regions. Moreover, the heterogeneity of these mutations can be used as genetic references to elucidate historical migrations and anthropological origins. The aim of this retrospective study, undertaken in the 16 prefecture-level divisions of Yunnan, was to determine the heterogeneity of thalassemia in different ethnic groups. To our knowledge, this is the first report on β-thalassemia mutations associated with the ethnic and regional variations in southwestern China. The results modify and expand the previously reported genetic information related to β-thalassemia in China.

Study Population and Detection of β-globin Mutations
From 2004 to 2014, a total of 1,441 individuals (aged 1-56 years) who were residents of Yunnan province were enrolled in the study. Hematological and clinical data associated with β-thalassemia obtained during genetic counseling, prenatal diagnosis and screening programs (programs conducted in the First Peoples' Hospital of Yunnan Province, Peoples' Hospital of Dehong, Maternal and Child Health Hospital of Wenshan, Maternal and Child Health Hospital of Xishuangbanna, Maternal and Child Health Hospital of Dehong, Maternal and Child Health Hospital of Lijiang) were analyzed. Blood samples were obtained for molecular analysis at either the First Peoples' Hospital of Yunnan Province or the Peoples' Hospital of Dehong Autonamic Prefecture of Dai and Jingpo. Clinical data were obtained from medical records of the patients and informed consent was signed by the participants or their guardians. The protocol and consent information for this study were approved by the Medical Ethics Committee of the First Peoples' Hospital of Yunnan Province, PRC. Venous blood samples were collected in tubes with EDTA from hematological phenotype positive carriers and patients. A total of 17 β-globin mutations frequently seen in the Chinese population were detected by PCR-reverse dot blot (RDB) assays as previously described [7]. The entire β-globin gene, from the 5' promoter region to the 3' untranslated region (3' UTR), were sequenced in cases that did not have any of the 17 common mutations or in cases in which the genotype disagreed with the phenotype according to a previous report [7].

Discussion
This is the first study on β-thalassemia carriers and other hemoglobinopathies covering 22 different ethnic groups from different regions of Yunnan. Knowledge of the geographic and ethnic distributions of β-thalassemia mutations has useful implications for the prevention and control of thalassemia in China. The data indicated that there were marked differences in the geographical distributions of β-thalassemia mutations within different racial groups, highlighting the ethnic diversity in Yunnan. Hb E (CD 26, G>A) (65.64%) and CD 41-42 (-TCTT) (30.5%) were the most prevalent mutations in the southwest and northeast regions, respectively, whereas CD 17 (A>T) (39.19%) was the most common mutation in the southeastern Yunnan population.
In the southwest region, Hb E (CD 26, G>A) (65.64%) was the most common mutation observed in the local minority groups, especially for the native people who live in the tropical plateau; i.e., Achang (100%), Jingpo (100%) and Deang (100%), which has not been reported in other populations in China. The high frequency of Hb E (CD 26, G>A) in this region was similar to that of adjacent countries, such as Myanmar [16], Thailand and Laos [13], where a "hot spot" of Hb E (CD 26, G>A) is located on the Thai-Laos-Cambodia border [17]. A plausible explanation for the high incidence of Hb E (CD 26, G>A) was that carriers of Hb E (CD 26, G>A) were protected against falciparum malaria [18]. The geographical distribution of Hb E (CD 26, G>A) in Yunnan co-exists with the distribution of previous malaria epidemics [19], suggesting that the high frequency of Hb E might be associated with natural selective inhibition of malaria [20]. Secondly, it may be due to high consanguinity in minority groups with very restricted population movement caused by sociocultural factors and difficult travel potential associated with being surrounded by large mountains, which increases the risks of β-thalassemia/ Hb E compound heterozygosity and Hb E homozygosity.
In the center region, where many different ethnic groups (14 ethnic groups in this study) reside, representing nearly 12 million habitants, genetic heterogeneity has led to a admixture of different β-thalassemia mutations. A total of 22 β-globin mutations, including 3 mutations that have not been reported previously in China, were detected in the 406 alleles, which accounted for two-fifths of the β-thalassemia mutations reported in China. Hb E (CD 26, G>A) (25.4%) and CD 17 (A>T) (24.6%) were the most common mutations. Among the mutations that were identified, CD 127 (A>G) was a very rare dominant β-thalassemia mutation first discovered in a 31-year-old French female 21 years ago [21]. Initiation CD (T>C) was observed in Yugoslavia in 1990 [22], but never in China. CD 47 (G>A) was a rare mutation detected in Danish, Sicilian and families with African ancestry [23]. The heterogeneity of β-thalassemia in this region might be due to the fact that the center of Yunnan, as a capital ruled by Yi, Mongols and Han people throughout history, is a crucial strategic crossroad between China and southern Asia. Also, the Japanese invasion (1940 AD) forced various immigrants into this region. Centuries of influx of many people resulted in great heterogeneity in the population. It has been suggested that the common mutations may be indigenous to the native population of Yunnan, whereas the rare β-globin mutations may have been introduced by immigration.
Our study has shown the remarkable diversity of β-thalassemia in different geographic regions in Yunnan. This information on the epidemiology of hemoglobin disorders could be helpful for planning appropriate treatments for β-thalassemia patients according to different population groups. Until now, there have been few studies regarding β-thalassemia carriers in minority groups and the evolution of haplotypes of the β-hemoglobin gene. Our study provided useful information to pave the way for prenatal diagnosis programs and large-scale population screening for β-thalassemia in Yunnan.