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LEUKOTRIENE B4: BIOLOGICAL ACTIVITIES AND THE CYTOSKELETON

1 The aggregation and chemokinesis of either rat or human polymorphonuclear leucocytes (PMNs) induced by leukotriene B4 isomer III (LTB4) are inhibited significantly by colchicine (10−6‐10−3m) and vinblastine (10−7‐10−4m). Random migration of the leucocytes is inhibited by colchicine 10−3m and vinblastine 10−4m. 2 Cytochalasin B (4 × 10−7‐4 × 10−6m) caused the aggregation of rat PMNs but inhibited their random migration. The aggregation of the PMNs induced by LTB4 was enhanced by cytochalasin B but the chemokinesis was inhibited. 3 It is suggested that both microtubules and microfilaments may be involved in the aggregatory and chemokinetic responses of PMNs to LTB4.