{'fraction_non_alphanumeric': 0.03112789789007554, 'fraction_numerical': 0.019731700963792655, 'mean_word_length': 4.798187653388711, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 1, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Fabrication of fibrillated and interconnected porous poly(ε-caprolactone) vascular tissue engineering scaffolds by microcellular foaming and polymer leaching

This paper provides a method combining eco-friendly supercritical CO2 microcellular foaming and polymer leaching to fabricate small-diameter vascular tissue engineering scaffolds. The relationship between pore morphology and mechanical properties, and the cytocompatibility, are investigated with respect to the effects of poly(ε-caprolactone)/poly(ethylene oxide) (PCL/PEO) phase morphologies and PEO leaching. When PEO content increases, the pore size decreases and the pore density increases. After the leaching process, highly interconnected and fibrillated porous structures are detected in the foamed PCL70 blend with droplet-matrix morphologies. Moreover, the leaching process had a greater contribution to improve the open-cell content in the PCL50 blend, which has a co-continuous morphology and easily obtained open-cell content of more than 80%. Small-diameter tubular PCL70 and PCL50 porous scaffolds with an average pore size of 48 ± 1.4 μm and 30 ± 1.0 μm respectively, are fabricated successfully. Prominent orientated pores are found in the PCL70 scaffold, and a mixed microstructure combining interconnected channels and open cells occurs in PCL50 scaffold. The PCL70 scaffold has a greater longitudinal tensile strength, longer toe region, and larger cyclical recoverability. HUVECs tend to align along the direction of the pore orientation in the PCL70 scaffold, whereas HUVECs have a higher density and spreading area in the PCL50 scaffold. The results gathered in this paper may provide a theoretical basis and data support for fabricating small-diameter porous tissue engineering vascular scaffolds.


Introduction
In recent years, tissue engineering, as a new treatment, has been used to improve the recovery of various types of tissue, such as bones, blood vessels, the oesophagus and skin. 1,2 The application of vascular tissue engineering technology holds great promise for improving outcomes in patients with peripheral vascular diseases and cardiac ischemia. 3 Although some progress has been achieved in developing vascular gras using materials such as expanded polytetrauorethylene (e-PTFE), generating vascular gras with a small diameter (#6 mm) is still challenging due to the risk of infection, thrombus, and calcication when implanted. 4 Therefore, the development of new scaffolds with a smaller vasculature diameter is imperative for the advancement of vascular tissue engineering.
As one of the most important elements of tissue engineering, the scaffold acts as a synthetic analogue of the natural extracellular matrix (ECM). To meet tissue engineering requirements, the physical topology of the scaffold should be a threedimensional (3D), highly porous structure with interconnected channels and hierarchical in pore size. 5 A highly porous and interconnected 3D structure will not only facilitate cell adhesion, migration, and reproduction into the interior part of the scaffold, but also can support the mass transport of cell nutrients and waste. 6 In fact, it is not easy to produce such high-porosity scaffolds with specied geometric shapes and the necessary mechanical properties. Being hierarchical in pore size refers to the various scales and their effects; for example, mesoscopic pores (>50 mm) inuence tissue shape, microscopic pores (1-50 mm) inuence cell function, and nanoscopic pores (<1 mm) inuence nutrient diffusion. 7 Therefore, how to produce a scaffold with pores that are highly open, interconnected, and a desirable size and have a uniform distribution is the key point of scaffold fabrication in tissue engineering.
Until now, several different techniques (e.g., solvent extraction, particle leaching, phase separation, electrospinning, rapid prototyping, and gas foaming [8][9][10][11][12] ) have been established based on biodegradable polymers to fabricate the ideal scaffold structure. Gas foaming is attracting more and more interest from researchers because of its advantages of being free of organic solvents and eco-friendly. 13,14 During this process, carbon dioxide (CO 2 ), which is as widely used as it is inexpensive, non-toxic, highly dissolvable in polymers, recoverable, and reusable, is rst dissolved into a polymer melt at a certain temperature and pressure. Aer the CO 2 turns to a supercritical uid (SCF), it is depressurised to ambient levels to trigger the nucleation. A porous structure then emerges due to the expansion of the CO 2 when it transitions from the supercritical to the gaseous state. However, this process is limited by the variety of pore size it can create, as well as their uncontrollable shape. In most cases, low interconnectivity results because that the gas expansion force is too small to overcome the strength of the polymer matrix. 15 Particle leaching is an effective technique for producing fully interconnected porous scaffolds. This technique blends water-soluble inorganic particles or polymers with one matrix material; a porous structure can be generated aer leaching the sacricial phase. The disadvantages of unpredictable pore sizes and poor mechanical properties have been detected during scaffold fabrication by a single particle leaching process. To further improve the porosity and interconnectivity of the scaffold, a scaffold fabrication method combining gas foaming and polymer leaching can be used and is attracting increased attention. 16,17 This mixed method can give scaffolds that perform better than those of a given individual process in tissue engineering applications.
Many synthetic thermoplastic aliphatic polymers, such as polylactide (PLA), polyglycolide (PGA), poly(3-caprolactone) (PCL), and poly(lactide-co-glycolide) (PLGA), have been commonly used to fabricate tissue engineering scaffolds because of their excellent biodegradability and biocompatibility. 18-20 PCL, a semi-crystalline polymer, has been a popular biomedical material for tissue engineering. PCL has good mechanical properties such as high exibility and elongation. The degradation time of PCL can reach up to 24 months by hydrolytic scission. 21,22 Most PCL scaffolds are fabricated by electrospinning. Poly(ethylene oxide) (PEO) is another biomedical polymer that is biodegradable, biocompatible, and water-soluble. PEO itself is non-toxic to cells and can be dissolved without toxic organic solvents. Therefore, any residual polymer will not affect the cell viability 23 This study prepares a highly interconnected, biocompatible and biodegradable, porous, tubular PCL scaffold with nely controlled porous microstructures. A solid-state supercritical CO 2 batch foaming process and a polymer leaching method are combined. The effects of the blend composition and phase morphology on the foaming behaviour and nal pore morphology, as well as the polymer leaching efficiency, are investigated. Pore size, pore density, and open-cell content are measured quantitatively. Furthermore, tubular PCL scaffolds with a desired pore size and highly interconnected porous structures are fabricated successfully. Related tensile properties and cytocompatibility are explored.

Materials
PCL (CAPA 6500, Perstorp) was used as the matrix material. PEO (WSR N-10, Dow) was chosen as the sacricial material due to its water solubility and similarity in melt temperature to that of PCL. Some characteristics of PCL and PEO are listed in Table 1. The PCL and PEO were stored in sealed containers to minimize moisture absorption and were further dried at 40 C under vacuum for 24 h prior to compounding.
The pre-blended pellets were hot embossed into thin membranes with a thickness of 200 AE 10 mm. Rectangular lms (20 mm Â 15 mm) were cut from the middle regions. A tubular mould was specially designed with an inner diameter (ID) of 5 mm, and assembled with one metal core with an outer diameter (OD) of 4 mm. One layer of aluminium-foil paper was stuck onto the inner mould wall to help with demoulding. Then, the lm was inserted into the mould to form a tube. Next, tubularshaped specimens (OD ¼ 5 mm, ID ¼ 4 mm) were placed into a high-pressure vessel made in-house that had been preheated to a saturation temperature (T sat ) for 1.5 h. The vessel was ushed with low-pressure CO 2 for 2 min using a syringe pump and then lled with CO 2 to the desired saturation pressure (P sat ) and hold for the desired saturation time (t sat ). The system, in which CO 2 had transitioned into the SCF, was kept in equilibrium at T sat , P sat and t sat to ensure full adsorption of CO 2 . The pressure was quenched to ambient pressure in a very short time (0.5-1 s), and the samples were quickly removed from the vessel. The saturation conditions for foaming were based on our previous research and were T sat ¼ 42 C, P sat ¼ 2000 psi, and t sat ¼ 1 h. The resulting foamed PCL/PEO scaffolds were immersed into deionized water to leach out the PEO. The water was changed every 6 h until a constant weight was obtained. Aer that, the wet scaffolds were dried in vacuum oven at 37 C for 24 h to remove any moisture. The whole process of preparing the porous PCL scaffolds was illustrated in Fig. 1.

Characterization and measurement
2.3.1 Morphology and pore size of scaffolds. The morphologies of the binary PCL/PEO blends and the microstructures of foamed clavate and tubular-shaped samples were characterized by a scanning electron microscope (FEI Quanta 200) with an accelerating voltage of 10 kV All samples were fractured at liquid nitrogen temperature. The SEM observations were made aer sputtering the samples with a thin lm of gold for 60 s at 2 mA.
The pore size and pore density were estimated by tracing a minimum of 100 pores from SEM micrographs using the Image-Pro Plus soware, which enables one to assess the diameter of each pore. The average pore diameter (D) of all the pores in the SEM micrograph was calculated with eqn (1): 24 where, n is the number of pores in the SEM micrograph, and d i is the equivalent diameter of one pore. The pore density (N f ) of the porous samples can be calculated by eqn (2): 24 where, N is the number of pores in the micrograph, and A is the area of the micrograph (cm 2 ).

Rheology.
Linear dynamic rheological measurements were performed on the neat PCL, PEO, and PCL/PEO blends by a strain-controlled rotational rheometer (DHR-2, TA) with parallel plates geometry (25 mm diameter plate) with a xed gap of 1000 mm. The strain sweep mode was pre-tested to nd a suitable strain range to ensure that all samples were tested in the linear viscoelastic region. In the frequency sweep mode, the strain amplitude was set at 1.5% (ESI Fig. S1 †). The dynamic frequency scans were carried out from u ¼ 0.05 to u ¼ 500 rad s À1 at 120 C under gaseous nitrogen ow to prevent thermooxidative degradation.
where r foam is the apparent density of the foamed sample, which was measured referring to the ASTM D792-00, m is the apparent mass of sample in air without the sinker, m 0 is the apparent mass of sample and the sinker fully immersed in water, and M is the apparent mass of the entirely immersed sinker. r polymer is the density of the PCL sample (1.1 g cm À3 in this study), V pore is the open-cell volume, which can be obtained using a helium pycnometer (ULTRAPYC 1200e, Quantachrome Instruments, USA), and V polymer is the total volume of polymer.

Longitudinal and radial tension.
The mechanical properties of the scaffolds were characterized via the longitudinal and radial tensile properties of the tubular scaffolds aer leaching the PEO All tests were done on a computer-controlled SANS tester (Model UTM 2203) with a 100 N load cell. As shown in Fig. 2(a) and (b), the xtures were specically designed for tubular scaffolds. The effective initial lengths for the longitudinal and radial tensile testing were 20 mm and 6.5 mm, respectively. A pre-tension of 0.1 N was applied to all samples. The extension rate of 5 mm min À1 was used during This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 10055-10066 | 10057 the tensile test until the sample failed. The broken stress and strain of each sample was tested based on ISO 7198:1998. The modulus of the sample was dened as the slope of the stressstrain curve at 5% elongation. Statistical results were the average of four samples. To avoid the possible reduction of the foam volume, all characterizations were carried out aer the foams were placed in the constant temperature and humidity chamber (23 AE 2 C, relative humidity of 40-50%) for 3 days.
2.3.5 Cell morphology and adhesion (cytoskeleton assay). The cell morphology aer 1, 3, and 5 days was characterized by CF568 phalloidin (red F-actin) staining The cells were xed with 4% formaldehyde for 10 min, penetrated with PBS containing 0.5% Triton X-100 for 10 min, then blocked with PBS containing 5% bovine serum albumin for 30 min, and washed with PBS three times. Cells were incubated with a phalloidin dilution (1/40 v/v dilution in PBS) overnight at 4 C, nuclear DNA was labelled with a 4 0 ,6diamidino-2-phenylindole (DAPI) dilution (1/10 v/v dilution in PBS), and cells were imaged with a confocal laser scanning microscope.

Results and discussion
3.1 Morphology of PCL/PEO blend SEM was rst used to characterize the phase morphology of neat PCL and the PCL/PEO blends. The micrographs were used to further understand the components' compatibility. Fig. 3 shows SEM images of the fracture surface for neat PCL and PCL/PEO blends with various blend ratios aer leaching. All samples exhibited typical binary structures, which further proved the immiscibility of PCL and PEO. Neat PCL (Fig. 3(a)) showed a relatively smooth fracture surface. Dispersed droplets of the minor phase in the matrix were observed with the addition of 20% and 30% PEO ( Fig. 3(b) and (c)) , respectively, and the fractured surface became slightly rough. The average size of the dispersed PEO phase increased from 0.8 mm to 2 mm when PEO content increased by 10%. As the content of PEO phase increased to 40%, the morphology changed signicantly. The dispersed phase clearly showed an elongated or brous structure ( Fig. 3(d)) and a partially co-continuous morphology emerged. Average size of dispersed PEO increased to 6 mm. The number of voids increased and voids interconnected with each other as the amount of PEO increased up to 50%. Continuous channels were clearly seen and were more uniformly distributed  throughout than in the samples with 40% PEO components (Fig. 3(e)). Channels on the void walls were visible, suggesting the formation of a fully co-continuous phase.

Rheological property
The foamability of polymeric material is strongly affected by its rheological behaviour. 26,27 Fig. 4 shows the dynamic storage modulus (G 0 ), complex viscosity (h*) and Cole-Cole curves obtained from rheological tests. A well-developed dependence was observed in that G 0 decreased with an increase in PCL content at all frequencies tested. The modulus approached the terminal behaviour, G 0 -u 2 , for neat PCL at low frequencies. A monotonic increase of G 0 in the blends was found with increasing PEO content. However, the growth of G 0 was not regular with the composition (as shown by the slightly different slopes of the dashed lines in Fig. 4(a)) when the PEO content was raised above 40 wt%. Such a rheological transition is due to the change of phase morphology during the oscillatory shear deformation. 28 While in a high-frequency region, the energy level was negligible compared with the elastic energy level required for the deformation within the polymer blends. So, all samples approximately show a common G 0 . The complex viscosity is usually considered a reection of melt strength. As shown in Fig. 4(b), an almost a typical Newtonian uid behaviour was exhibited for the neat PCL. The phenomenon of shear-thinning increased greatly with an increase of PEO content, and the PEO exhibited typical pseudoplastic uid behaviour. The viscosity of PCL/PEO blends fell between those of the PCL and PEO. It was efficient to improve the melt strength by adding PEO, which favoured the formation of open-pore structures. Fig. 4(c) shows the Cole-Cole plots of imaginary viscosity (h 00 ) versus real viscosity (h 0 ) for all components. The homogeneous polymers exhibited only one circular arc in the curve, while a tail appeared on the right-hand side of the arc for all blends. An upward trend of the curve in high-viscosity region was observed for the blends PCL60 and PCL50, and a second circular-like arc was formed. Such plot behaviour demonstrated that there were two different relaxation mechanisms corresponding to the two different phase morphologies, 29 in agreement with the SEM micrographs.

Morphology of scaffolds before and aer leaching PEO
The effect of the PEO concentration on the morphology of the PCL foams before leaching PEO is shown in Fig. 5. Neat PCL exhibited a huge circular pore structure, and most of the pores had a closed-pore structure. As seen in the SEM micrographs Fig. 5(b)-(e), all pores were characterized by a sharply decreased size and an open-pore morphology. The shape of the pores changed from having regular polyhedral faces ( Fig. 5(a)) to being a fully random polygon (Fig. 5(d) and (e)). Increasing the concentration of PEO from 20 wt% to 50 wt% increased the thickness of pores and decreased the number of wellinterconnected pores. Fig. 5(d) shown that nonuniform pore distribution were found and many pores seems be "collapse". That was may due to the phase morphology of PCL60 blend. We could see from the Fig. 3 showed an elongated structure and a partially co-continuous morphology. The non-uniform dispersion of PEO phases lead to inhomogeneous nucleation during foaming. Non-uniform PEO phases combined with PCL made the pore wall more thicker. Various interface bonding between PEO and PCL made the pore wall collapse-like and unsmooth. When the PEO was increased to 50 wt%, more unfoamed regions were detected compared with PCL60 samples. Another feature exhibted from the morphologies of PCL/PEO foamed samples was nonhomogeneous structures detected with a narrow pore size distribution because of the various foaming behaviors of PCL and PEO. To further characterise the pore structure, the average pore size and pore density were measured, as shown in Fig. 6. The plot shows that the average pore size decreased from 79.5 mm to 8.2 mm, while the average pore density increased from 1.5 Â 10 5 to 8.9 Â 10 8 cells per cm 3 , as the PEO content increased.

(d) that the dispersed PEO phase
These results occured because the heterogeneous nucleation effect was strengthened aer PEO was added, which increased the number of nucleating points and restricted the expansion of cells. 30,31 Fig. 7 exhibits the morphology of foamed PCL/PEO samples aer efficient leaching of the PEO (ESI Fig. S2 †). All samples showed a highly interconnected pore structure with holes fractured in the pore walls. Fully random polygon pore shape were also found. The pores were interconnected with many brillated structures at the nano-scale for the PCL80, PCL70 and PCL60 foamed samples. This result could be explained from a phase morphology point of view. The matrix phase was PCL, which showed good strength in PCL80 and PCL70 blend systems, as shown by SEM. The pore walls of PCL were stretched to a certain extent during cell expansion. Under the function of CO 2 -induced plasticisation, the thickness of the pore walls decreased, and the walls resisted the dispersion of PEO with its higher melt strength. The matrix PCL became too weak to sustain cell expansion, and then the pores tended to break, which resulted in open pores and brillated structures among the pore walls. Such structures have been found in other research papers and were useful for enhancing cell adhesion during cell culturing. 32,33 Many continuous channels appeared aer leaching PEO for PCL50 samples. Multiple morphologies of open porous structures appeared. For the pre-leached PCL50 scaffolds, pore morphology characterized with small pore size and high pore density. Probably many PEO phases located on the pore walls. Even some pore walls were only made of PEO material. In that case, the continuous channels shown in Fig. 7(e) emerged aer leaching process. Some channels came from the co-continuous PEO phase, and others were attributed to the connected PCL pores aer the PEO on the pore walls were  leached out. Such continuous channels combined with cellopening PCL pores built a highly interconnected porous structure, which would probably enhance cell adhesion and migration during cell culturing.

Overall porosity and interconnectivity of scaffolds
The statistical results of porosity are shown in Fig. 8(a). As expected, increasing the concentration of PEO induced an increase in overall porosity. The porosity increased from 67% to 88% with an increasing concentration of PEO from 0% to 30%. The porosity decreased slightly for the PCL60 foamed samples, which may be the fact that it was not possible to leach out the PEO phase. For the PCL50 foamed sample, the porosity can reach 89% because of leaching process. Fig. 8(b) depicted the open-pore content of PCL/PEO foamed samples before and aer leaching. In contrast with the porosity, the open-pore content increased rst and then decreased as the amount of PEO increased. Before leaching the PEO, a maximum open-pore content of 78% was obtained with PCL70. Because a cocontinuous morphologies emerged when the concentration of PEO was more than 30%. The interface bonding force between PCL and PEO phases was too large to help cell nucleation and cell growth, 34,35 so that it would be difficult to initiate the opening of a cell at the cell wall because of the higher melt strength. Aer the leaching process, the open-pore content was obviously improved for all the foamed samples, and the maximum porosity of 85% was detected with PCL70. Additionally, the average increment of open-pore content was about 5% aer leaching for the PCL80 and PCL70. However, the average increment of open-pore content increased by 11% when the concentration of PEO added changed from 30% to 50%. This phenomenon indicated that the method of blending the immiscible polymers had a big effect on the interconnectivity of pores when the content of PEO was low. However, the leaching process contributed more to the open-pore content of the porous scaffolds aer the co-continuous morphologies of the PCL/PEO blends emerged.

Morphology of small diameter porous PCL tubular scaffolds
PCL70 and PCL50 blends were selected to be foamed into micro porous tubular scaffolds. As illustrated in Fig. 9, uniform  This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 10055-10066 | 10061 tubular porous scaffolds were obtained with the same foaming process for both the PCL70 and PCL50 samples (ESI S3 †). Generally, the pore size showed a gradient change across the thickness of the tube, as in "skin-core" microstructures. A number of pores with diameters of greater than 30 mm were located in the core zones of the samples, while some pores smaller than 10 mm were detected near the inner and outer surfaces of the scaffolds. This non-uniform pore gradient tendency corresponded to the restricted surfaces of scaffolds. In order to obtain homogeneous pore distribution, pore size could be increased by improving foaming temperature. 9 Meanwhile, additional process, surface etching or ultrasonic treatment, 36,37 could be used to improve the porosity of outer and inner surface of scaffolds. The non-uniform adsorption and desorption characteristics of ScCO 2 in polymers prompt the nonhomogeneous nucleation and growth of pores. 38 Comparison the pore morphologies for both foamed samples in the core regions showed that pore size distributions were similar.
The average pore size and open-pore content were 48 AE 1.4 mm and 72 AE 2.1% respectively, for porous PCL70 scaffolds. Prominent orientations of pores perpendicular to the thickness of the tube were clearly seen, and the aspect ratio of pores was about 3 : 1. Related reports have found that a certain degree of orientation in porous structures is efficient for the growth and migration of cells in the tissue engineering eld. 24,38 The pore morphology for the PCL50 porous scaffolds differed greatly from that of PCL70. No regularly oriented pores were found because the cocontinuous PEO phases hindered the orientation of cell nucleation and growth. The average pore size decreased to 30 AE 1.0 mm, but the open-pore content improved to 79 AE 1.9% compared with foamed PCL70. This changes can be explained by the fact that interconnected channels were formed aer the PEO leaching process. The interconnected channels together with the open pores during cell growing build one mixed microstructure which enhances the total open-pore content of the PCL50 foamed sample.
To produce foams with high open-pore contents, an efficient strategy is to melt-blend two semi-crystalline polymers with different crystallization temperatures (T c ) to maximize the stiffness contrast between the hard and so regions in the polymer matrix. 39 The cell-opening mechanisms for the PCL70 and PCL50 tubular porous scaffolds are illustrated in Fig. 10. In the same conned space, two possible cell-opening mechanisms were proposed depending on the polymer blend and its phase morphology. As shown in Fig. 10(a), PCL with a low-T c formed matrix which was so, and PEO with a high-T c formed hard dispersed phases (ESI S4 †). Theoretically, cell nucleation oen occurs in the PCL matrix and around the PCL/ PEO interface, preferentially where there are higher gas concentrations and lower activation energy barriers. 40 Because of the different stiffnesses and shrinkages of PCL and PEO, the debonding process between the so PCL matrix and hard spherical PEO domains initiated cell opening during cell growth. Fig. 10(b) exhibits the cell opening mechanism for PCL50 with its co-continuous phase morphology. More heterophasic nucleation points appeared in the PCL matrix, other than on the PCL/PEO interface, compared with in PCL70 because of the higher interface bonding force. More open cells were generated by the same cell-opening strategy as in foamed PCL70. However, the leaching process contributed more to the open-pore content. On one hand, the leached PEO phases drag the surrounding PCL cells. On the other hand, long channels originating from the leached PEO occurred. This mixed porous microstructure that combines PCL's open cells and interconnected channels can improve the open-pore content of foamed PCL50 samples.

Mechanical properties
The tensile properties in the longitudinal and circumferential directions signicantly inuence the application of vascular tissue engineering scaffolds. The device stiffness and strength are important characteristics that dene the interface with existing tissue and the pressure tolerance of the vascular scaffolds. 41,42 Fig. 11(a) shows that the longitudinal strength and modulus of foamed PCL70 was bigger than that of PCL50. PCL70 also showed a slightly higher longitudinal elongation value than that of the PCL50 scaffold. This result is due to the large wall thickness and brillation among the cell walls for foamed PCL70. Both scaffolds meet the requirements of a human's brain veins. 43,44 The toe region is one characteristic of the mechanical properties of a blood vessel. 45 The circumferential stress-strain curve indicates that the toe region of the PCL70 porous scaffolds was around 25%, while that of PCL50 was only less 10%. This change was attributed to cell deformation during the tensile process. The evolutions of cell deformation for the PCL70 and PCL50 samples are illustrated in Fig. 11(c). At the beginning of the bulk deformation of the PCL70 microtube, some orientated ellipsoidal-shaped cells rst deformed to a spherical shape, prior to a decrease of the cell wall thickness along the tensile force direction. That strain process needs only a little stress to have toe regions form. Since no orientated cells and only open cells combined with interconnected channels existed in the PCL50 scaffold, the wall thicknesses of the channels and irregular cells decreased rapidly. This means that the tensile stress can increase sharply without having a toe region.
Cyclical properties are a critical attribute of vascular tissue engineering scaffolds because blood vessels are constantly subjected to blood pressure. 46 The cyclical tensile properties of PCL70 and PCL50 were characterized in the circumferential direction for 30 cycles. Fig. 11(d) shows that both the PCL70 and PCL50 scaffolds showed a larger hysteresis loop in the rst cycle, and the hysteresis stayed the same in the following cycles. This result demonstrates that the energy dissipation and structure relaxation mostly happened in the rst cycle. Moreover, the PCL70 scaffolds exhibited a relatively small initial energy dissipation and larger recoverability that could be maintained in the cyclical tests. The PCL50 scaffolds, however, showed poor recoverability because of the low elongation. Hence, PCL70 could better resemble the nonlinear mechanical properties in native blood vessels than could the PCL50 scaffold, even with its smaller open-pore content.

Cell morphology
The structure of a cytoskeleton can reect the proliferation of cells and the cell morphology evolution to a certain extent. The nucleus is well known for containing the cell's regulatory genes, which can control protein expression and affect cell behaviors. 47 Fig. 12 shows the cytoskeleton and nuclei of HUVECs cultured on PCL70 and PCL50 porous tubular scaffolds at days 1, 3, and 5. The gure shows that with increasing culture time, the cell morphologies on both porous scaffolds tended to be more complete and mature. The nuclei were at and liable to spread more fully, indicating that both high-interconnectivity porous scaffolds had a strong interaction with cells and were favourable for cell attachment and proliferation. 48 Additionally, the cytoskeletons of HUVECs on the PCL70 scaffold were stretched along certain a orientation direction, and the nuclei tended to have spindle-shaped growth. This results could be attributed to the fact that the highly interconnected porous structure with brils shown previously in Fig. 9 (top row, right image), can induce directional cell growth. Compared with the PCL70 scaffold, HUVECs on the PCL50 scaffold had higher density and