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Action of ketotifen on histamine release and intracellular cAMP levels in basophil cultures from pollinic subjects.

Basophils from peripheral blood of 22 pollinic patients were isolated and were cultured in the presence of different concentrations of ketotifen. We studied the effect of this drug on the specific histamine release (Technicon autoanalyzer) and the levels of intracellular cAMP during days 1, 6 and 12 of culture period. We observed that ketotifen induced an inhibition of specific histamine release throughout the culture days, but was greater on the 12th day. The cAMP levels likewise increased during the period of culture in the presence of ketotifen.