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[Studies on immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum].

OBJECTIVE
To analyze the immunological reaction of the antiserum of recombinant secreted protein from Ancylostoma caninum with antigens of various species hookworms at different developmental stages.


METHODS
SDS-PAGE and ELIB technique were employed in the study.


RESULTS AND CONCLUSION
The protein component of Ac-rAsp-1 was 45 kDa, its immune serum can recognize the antigens of Ac-L3 and Ac-rAsp-1 protein, but not react to the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Nb-A and Ac-rAsp-2 protein. The protein component of Ac-rAsp-2 was 24 kDa, its immune serum can recognize the antigens of Ad-A, Ad-L3, Na-A, Ac-A, Ac-L3 and Ac-rAsp-2 protein, but not react to the antigens of Nb-A and Ac-rAsp-1 protein.