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Changes in vitality of populations of rare arctic-alpine plant species in high mountain part of the Ukrainian Carpathians under the influence of climatic factors

© 2019 R.M. Cherepanyn. Published by the Ivan Franko National University of Lviv on behalf of Біологічні Cтудії / Studia Biologica. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://www.budapestopenaccessinitiative.org/ and Creative Commons Attribution 4.0 License), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. UDC 581.5:574.3]:57.045(234.421.1:477)

Changes in vitality of populations of rare arctic-alpine plant species (Anemone narcissiflora, Bartsia alpina, Saussurea alpina, Pedicularis oederi) of the Ukrainian Carpathians in the highland habitats under the influence of climatic factors were analyzed. Population vitality was investigated according to individual diagnostic parametersvitality of individuals, population vitality index, population quality index, and according to population parameters -density, total and effective quantity.
It was established that during 2010-2018, the vitality of the Saussurea alpina population on the Shpyci Mount remained average. The uneven distribution of individuals on habitat area and complex spatial structure of the population loci positively affected the vitality of the Saussurea alpina population on Mt. Shpyci. Individuals of different vitality levels and heterogeneous structure according to morphometric parameters were observed in population.
A decrease in population vitality and flowering gaps of generative individuals were observed in Saussurea alpina population on Mt. Petros due to climatic changes, intrapopulation processes, and anthropogenic stress.
The upper locus of the only population of Pedicularis oederi in Ukraine between Brebeneskul and Munchel mountains underwent transformation, in particular, drying out of suitable habitats due to climate change. The population also underwent periodic anthropogenic influence as a result of grazing, which leads to unstable demographic trends -decrease in the habitat area, number of adults and generative individuals, coefficient of generative reproduction etc.

INTRODUCTION
Population viability is a general characteristics of self-recovery, settlement and preservation of evolutionary perspectives -remains an important aspect in studying of changes that occurr in populations of rare plant species under the influence of climatic factors. The problems of populations viability and vitality were considered by many researchers who developed various approaches [3,4,[18][19][20]22]. A number of works were devoted to assessing the populations viability of rare plant species in the Ukrainian Carpathians [1,26,15,24,29]. Detailed study of mechanisms that ensure population viability remains relevant in future. Establishing their vitality is an integral characteristic that reflects current state of populations on the basis of the most important individual and group parameters (structure, dynamics, evolution and reproduction) [16].
Vitality can be defined as an indicator of individuals or populations state that is charac terized by qualitative development parameters and quantitative growth parameters [21]. In particular, the vitality of populations depends on the age spectrum, reproductive efficiency, population structure due to the vitality of individuals etc. The populations vitality depends on spatialfunctional integrity, natural and anthropogenic influence [2,12,13]. Diagnostic indicators that characterize the vitality of small populations are intrapopulation diversity, habitat area, spatial structure, population dynamics, seed productivity, density of individuals, total and effective quantity [7,8,14,26].
The aim of this study was to analyze changes in the populations vitality of rare arctic-alpine plant species in the high mountain part of the Ukrainian Carpathians under the influence of climate changes. Arcticalpine species play an important role in ecosystems of Arctic and sub-Arctic regions and in mountains of the Northern Hemisphere. These species also serve as model organisms in study related to the analysis of effects of climate change on the environment.

MATERIALS AND METHODS
The following rare arcticalpine plant species in high mountain part of the Ukrainian Carpathians were selected in order to analyze changes in populations vitality under the influence of climate changes: Anemone narcissiflora L., Bartsia alpina L., Saussurea alpina (L.) DC., Pedicularis oederi Vahl.
A study was conducted by stationary and route methods in the Chornohora massif. The area of the habitat, height above sea level, density and number of individuals were determined for populations. Population density was determined by a number of individuals per area, in terms of 1 m² [10]. Age structure of populations was determined on the basis of age groups ratio of individuals [9,17,25].
The coefficient of generative reproduction in populations was defined as a ratio of number of generative individuals to adult individuals. A recovery index in populations was calculated as a relation in number of pregenerative individuals to generative individuals [23].
Population vitality was studied according to individual diagnostic parameters -individuals' vitality, population vitality index, population quality index; and according to population diagnostic parameters -density, total and effective quantity etc. The vitality of individuals was studied according to a traditional three-level graduation of individuals' vitality in vitality classes. We differentiated the vitality of individuals to "high" (c<Q), "average" (c = Q) and "low" (c>Q), using the population quality index (Q), calculated as: Q = 0.5(a+b), where "a", "b" and "c" are the frequencies of individuals of high, average and low vitality [24,[26][27][28]. Also, we used the index of coenopopulations vitality (IVC), developed for rare plant species, for analysis of population vita lity. It was calculated as: where xi -is the average value of the i-th sign in the population, Xi -is the average value of the i-th sign for all populations and N -is the number of signs [4,11].
The names of plant species are given according to "Identification key for plants of the Ukrainian Carpathians" and "The determinant of higher plants of Ukraine" [5,6].

RESULTS AND DISSCUSION
The population of Saussurea alpina on the south-east slopes of Mt. Shpyci (Chornohora massif) at the altitude of 1840-1860 m above sea level consists of two loci and grows in rocky plant communities (alliance -Oxytropido-Elynion, associations -Caricetum sempervirentis and Thymo-Festucetum amethystinae) which change not significantly under influense of climate factors. The vitality of the population remains average during 2010-2018 (Table 1). The total area of the Saussurea alpina population is 300 m². The total population quantity is about 350 adults, the effective population quantity increased from 80 generative individuals in 2010 to 120 -in 2018. In particular, the number of generative individuals increases from 29 pcs. to 61 pcs. in the first loci at 1860 m a.s.l. An increase in the number of generative individuals from 50 to 60 pieces was observed in the second locus at 1840 m a.s.l. The number of adult individuals in the first and second loci was 100 and 250 pieces, respectively. The density of individuals reached 15-20 pieces per 1 m². The coefficient of generative reproduction has increased from 19 to 34 % over the period of study, while the recovery index has decreased from 3.3 to 1.7.
A number of generative individuals and the coefficient of generative reproduction increased due to a transition of a group of individuals from the virginile to the generative stage of ontogenesis. A reduction of the recovery index is due to the dominance of clonal reproduction in the population and a decrease in number of suitable sites for seeds germination, because of their overgrowth by the highdensity species (Carex sempervirens Vill.) (Fig. 1). The uneven distribution of individuals on the habitat area and complex spatial structure of the population loci positively affected the vitality of the Saussurea alpina population on Mt. Shpyci. The individuals of different vitality levels and heterogeneous structure according to morphometric parameters were observed in the population ( Table 2, 3).
A decrease in population vitality and flowering gaps of generative individuals were observed in Saussurea alpina population on the north-eastern slope of Mt. Petros at 1820 m a.s.l. which grows in conditions of rocky coenoses. In particular, since 2010, there was a sharp decrease in number of flowering individuals. In 2009, 60 blooming individuals were detected in the population however, only 5 blooming individuals were  Table 3. The upper locus of the only population in Ukraine of Pedicularis oederi in between Brebeneskul and Munchel mountains located on a wet meadow on a south-west slope at 1955 m a.s.l. underwent transformation, in particular, drying out of suitable habitats due to climate change ( Table 4). The population also underwent periodic anthropogenic influence as a result of grazing. The locus consists of two fragments are located at a distance of about 20 m of each other. In 2011, the area of the first fragment was 150 m², the area of the 2nd fragment -50 m². In this habitat, the total quantity of individuals was 250 adults (150 in the first fragment and 100 in the second), the effective number was 70 generative individuals (40 -in the first fragment and 30 -in the second). The density of generative individuals in the population ranged from 2 to 7 per 1 m², vegetative individuals -4-6 per 1 m², seedlings -4-6 per 1m². Immature and juvenile individuals, as well as a small proportion of senile and subsenile individuals (up to 10 %) were observed in the population. The height of the generative shoots were from 10 to 18 cm, the number of fruits on the generative shoots are from 8 to 15 pieces. The coefficient of generative reproduction was 28 %, the recovery index equaled 2 in the upper loci of Pedicularis oederi population.
The area of the first and second fragments of the upper locus of the Pedicularis oederi population decreased significantly as a result of drying out of the habitat in 2017 (Fig. 2). In particular, the area of the first fragment is 90 m², thus, decreased by 40 %, the area of the second fragment is 35 m², thas, decreased by 30 %. Also, the total number of individuals was decreased by 40 %, and the effective number of individuals was decreased by 70 %. In particular, the total number were 150 adults individuals (100 in the first fragment and 50 -in the second one), the effective number is 20 generative individuals (15 -in the first fragment and 5 -in the second one). The number of subsenile and senile individuals increases (up to 30 %) and the density of generative individuals decreases (1-3 per 1 m²) in population. The coefficient of generative reproduction was 10-15 %, the recovery index was 5.5.
Pedicularis oederi propagates only by seeds, therefore, the populations vitality depends on the efficiency of generative reproduction and the area of suitable habitats. During the vegetation season in 2018 a sufficient humidification of the habitat, significantly increasing number of flowering individuals (60 pieces in the first fragment and 30 pieces -in the second), increasing the coefficient of generative reproduction to 60 % are observed in the upper loci of Pedicularis oederi population. Unstable demographic trends in the population -flowering outbreaks and flowering decline, a significant fluctuation in number of pregenerative individuals and the recovery index (Table 4) due to dependence of populations' self-maintenance and self-recovery primarily on substrate moisture and availability of suitable locus for germination. Further existence of the upper locus of the population may be at risk under the conditions of habitat drying out and overgrown. The Anemone narcissiflorа population on the south-west slope behind Nesamovyte lake is located at an altitude of 1700-1800 m a.s.l. in rock and meadow phytocoenoses conditions, in particular, in Saxifrago-Festucetum versicoloris and Thymo-Festucetum amethystinae associations. The population belongs to normal type according to the age structure ( Table 5). The location of individuals is compactdiffuse in the habitat. The coefficient of generative reproduction varies within 40-60 %, which indicates high population vitality. The height of generative individuals in the populations is 26-31 cm. The number of generative shoots on a generative individual from 3 to 8 pieces. The density can reach 4-6 adults per 1 m² (2-4 generative individuals per 1 m²) in the population behind the Nesamovyte lake. Seed growth was detected in the population. The number of individuals in generative group is higher than one and a half times the number of individuals in pregenerative group. The population vitality is high in this habitat. Demographic parameters of population changed slightly during 2011-2018. The total number has increased due to subsenile and senile individuals. The number of pregenerative individuals and the effective number of population have decreased (Table 5). Population of Bartsia alpina on the Mt. Rebra is located on screes and characterized by the compactdiffuse distribution of individuals in the habitat. The population belongs to a normal type, with the leftside age spectrum (Table 6)  This reduces the area of free niches for seeds germination (Fig. 3). The density of adults, pregenerative and generative individuals is 32, 18 and 6 individuals per 1 m 2, respectively, in the population on the Mt. Rebra. The coefficient of generative reproduction va ries from 10.7 to 19.3 %. High values of individuals density and recovery index are due to the predominance of vegetative reproduction, which is intensified in the conditions of highdensity plant communities and overgrown of screes. Intensification of the polyvariability of the Bartsia alpina population ontogenesis is also observed in such conditions. A part of pregenerative and generative individuals is formed as a result of vegetative growth. Vegetative mobility of the individuals is 3-5 cm per year in the population. Rejuvenation of populations and an increasing of pregenerative phases duration, in particular -immature and virginile periods, are observed (Fig. 4). Such process has an important adaptive and evolutionary value, since a transition to the generative phase of ontogenesis occurs only in conditions favorable for this. Climate changes do not significantly affect the population vitality of rare arctoalpine plant species of rocky coenoses of a high mountain part of the Ukrainian Carpathians. In particular, the number of generative individuals was increased and a recovery index was decreased due to a predominance of vegetative reproduction and overgrowth by Carex sempervirens, that leads to a decrease in number of suitable sites for seed germination in Saussurea alpina population on Mt. Shpyci. The vitality of Anemone narcissiflorа population on the rocks behind the Nesamovyte Lake remains high, and the population vitality of Saussurea alpina on the Mt. Shpyci remains average.
Drying out of marshes and wet meadows leads to a decrease in the vitality of Pedicularis oederi population only in Ukraine. In particular, unstable demographic trends were observed in the population -the area of the habitat, the number of adults and generative individuals, the coefficient of generative reproduction have reduced.
The vitality parameters of Bartsia alpina population have been changed due to an overgrowth of the screes on Mt. Rebra by Juncus trifidus, Vaccinium myrtillus and Rhododendron myrtifolium. In particular, vegetative reproduction was increased as a result of a decrease in the area of free niches for generative reproduction. This leads to polyvariability of ontogenesis, an increase of the recovery index and a number of the pregenerative individuals, as well as a decrease in number of generative individuals.

ACKNOWLEDGMENTS
Publication is based on studies supported by the grant of the State Fund for Fundamental Research of Ukraine, No Ф76/92018.