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Detection and Analysis of a New Type of Cystathionine-γ-synthase in Nicotiana tabacum

By application of the reverse transcription PCR(RT-PCR) in small amount of cells,a new type cystathionine-γ-synthase(CGS) gene was found in tobacco zygote,named as Nt CGS-4.The analysis showed that the CDS sequence of Nt CGS-4 was 1 611 bp,encoding a polypeptide of 537 amino acid residues with a predicted molecular weight of 57.931 2 k u and the theoretical PI was 6.15. 2 696 bp sequence of the 5′ flanking region(promoter and 5′ UTR) of Nt CGS-4 was obtained by genome walking.The phylogenetic tree revealed that Nt CGS-4 had a high homology with CGS gene from multiple crops. The new type cystathionine-γ-synthase(CGS) gene Nt CGS-4 may be involved in the biosynthesis and metabolism of methionine in early embryogenesis of tobacco.