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Huangshan Floral Mushroom Polysaccharide Ameliorates Dextran Sulfate Sodium-Induced Colitis in Mice by Modulating Th17/treg Balance in a Gut Microbiota-Dependent Manner.

SCOPE
Ulcerative colitis (UC) is a common chronic recurrent inflammatory bowel disease. This study attempted to reveal the improvement mechanism of floral mushroom polysaccharide (FMPS) on UC from the perspective of coordinated interaction between intestinal microbes and intestinal helper T cell 17 (Th17)/regulatory T cell (Treg) balance.


METHODS AND RESULTS
Dextran sulfate sodium (DSS)-induced colitis mice model was used for the experiment. The results suggested that FMPS up-regulated the expression of occludin, ZO-1 and MUC2, and down-regulated the secretion of TNF-α, IL-1β and IL-6 in colitis mice. Importantly, FMPS restored intestinal Th17/Treg balance. Meanwhile, FMPS could regulate intestinal microorganisms and improve the level of short-chain fatty acids (SCFAs) in colitis mice. Intestinal microbial depletion and fecal microbiota transplantation (FMT) experiments revealed that FMPS ameliorated UC was mediated by intestinal microbiome. Flow cytometry further proved that FMPS restored intestinal Th17/Treg balance in a microbial-dependent manner.


CONCLUSION
These results indicated that FMPS has the potential to improve UC, and its mechanism depended on the restoration of Th17/Treg balance mediated by intestinal microorganisms. Therefore, it was suggested that FMPS dietary supplement can be potentially used to intervene UC. This article is protected by copyright. All rights reserved.