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Separation of olein-stearin from crude palm oil at different temperatures and solvents by centrifugation.

This project is concerned with separation of olein-stearin from crude palm oil (CPO) by centrifugation at different temperatures (ambient temperature, 0, -25 and -80 O C) and solvents (acetone, 2-propanol, 1-butanol and diethylether). Melting characteristics, triglycerides contents and fatty acid compositions in CPO and stearin fractions at the different conditions were analyzed. Since CPO samples at temperature below 0 O C were in solid state, separation by centrifugation could not be carried out. Also, it was difficult to maintain the solutions at the given temperatures throughout an experiment, therefore, effects of solvent polarity were performed only at ambient temperature. Among the experimental conditions, the highest percent yield of stearin (12.03%) was obtained from CPO dissolved in 2-propanol, while 10.18% was from CPO sample. Triglyceride is a major constituent (92.5-100%) in all the separated stearins. Studying on melting characteristics indicated that the stearin obtained form CPO at ambient temperature has the lowest melting point, while the others do not correspond with solvent polarity index. In addition, analysis of fatty acid using GLC with capillary column showed that CPO sample might comprise of some fatty acids which are not commonly found in biological materials and is under determined in this laboratory.