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Uptake and Metabolism of Choline by Rat Brain After Acute Choline Administration

Abstract: The present study is concerned with the uptake and metabolism of choline by the rat brain. Intraperitoneal administration of choline chloride (4‐60 mg/kg) caused a dose‐dependent elevation of the plasma choline concentration from 11.8 to up to 165.2 μM within 10 min and the reversal of the negative arteriovenous difference (AVD) of choline across the brain to positive values at plasma choline levels of >23 μM. Net choline release and uptake were linearly dependent on the plasma choline level in the physiological range of 10‐50 μM, whereas the CSF choline level was significantly increased only at plasma choline levels of >50 μM. The bolus injection of 60 mg/kg of [3H]choline chloride caused the net uptake of > 500 μMol/g of choline by the brain as calculated from the AVD, which was reflected in a minor increase of free choline level and a long‐lasting increase of brain phosphorylcholine content, which paralleled the uptake curve. Loss of label from phosphorylcholine 30 min to 24 h after choline administration was accompanied by an increase of label in phosphatidylcholine, an indication of a delayed transfer of newly taken‐up choline into membrane choline pools. In conclusion, homeostasis of brain choline is maintained by a complex system that interrelates choline net movements into and out of the brain and choline incorporation into and release from phospholipids.