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Towards an understanding of viroid nature and replication.

In leaf strips and in nuclei isolated from potato spindle tuber viroid (PSTV)-infected tomato, incorporation of [3H]UMP into PSTV is shown to be inhibited by pre-treatment of the leaf-strips with actinomycin D. These results demonstrate that PSTV is synthesized in the cell nucleus and that replication may occur from a DNA template.