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DEPLETION OF SPECIFIC GRAFT-VERSUS-HOST REACTIVITY FOLLOWING ADSORPTION OF NONSENSITIZED LYMPHOCYTES ON ALLOGENEIC FIBROBLASTS

SUMMARY Antigen recognition in transplantation reactions induced in vitro by mouse fibroblasts manifests itself in specific binding of T lymphocytes to membrane antigens of fibroblast monolayers. To deplete graft-versus-host (GVH) reactivity we interacted normal mouse lymphocytes with allogeneic fibroblasts; 4-6% of H-2d lymphocytes adhered to H-2k or H-2b monolayers. The nonadhering cells showed a reduced capacity to bind to cells of the original adsorbing H-2 phenotype. However, adherence to fibroblasts of a different H-2 was not impaired. The capacity of the nonadherent cells to evoke GVH reactivity in vivo was measured by assaying (1) splenomegaly of X-rayed animals injected with the nonadherent lymph node cells; (2) cytotoxicity to target cells of the repopulated spleen cells; and (3) enlargement of the draining lymph nodes in a local GVH reaction following injection of nonadherent spleen cells. All of these test systems showed that GVH reactivity was markedly decreased or eliminated in an immunospecific manner by adsorption. Specific adherence demonstrated the diversity of T lymphocytes with regard to cell receptors for alloantigens. This approach may provide a method for the specific elimination of antihost-reactive cells in the transplantation of bone marrow or lymphoid cells.