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Molecular identification of Brucella abortus and its virulence genes (bvfA, virB, and ure) in infected humans and cattle from Al-Diwaniyah province, Iraq

Materials and methods: According to that aim, 35 human-blood and 50 cattle-milk samples were collected from Al-Diwaniyah Teaching Hospital and local herd fields, respectively, from Al-Diwaniyah province, Iraq. A polymerase chain reaction (PCR) technique was employed to determine the identity of the bacterium and the presence of its VGs in the samples. Moreover, a partial gene sequencing (PGS) method targeted the 16S rRNA gene was used to confirm the infection by B. abortus in the samples.