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DNA vaccination targeting macrophage migration inhibitory factor prevents murine experimental colitis

Previous studies have shown that neutralization of macrophage migration inhibitory factor (MIF) by anti‐MIF antibody reduces intestinal inflammation in mice. In this study we tested whether or not anti‐MIF autoantibody induced by DNA vaccine targeting MIF protects mice against experimental colitis. Mice were administered a MIF‐deoxyribonucleic acid (DNA) vaccine by introducing oligonucleotides encoding helper T epitope into the cDNA sequence of murine MIF by in vivo electroporation. Preventive effects of this method against dextran sulphate sodium‐induced (DSS) colitis were evaluated. Mice administered with MIF‐DNA vaccine raised values of autoantibody significantly. The clinical and histological findings of colitis induced by 3·0% DSS solution were ameliorated significantly in mice treated with MIF‐DNA vaccine compared with saline or pCAGGS‐treated mice given DSS. Myeloperoxidase activity, infiltration of F4/80‐positive staining cells and the levels of proinflammatory cytokines were suppressed in the colon of MIF‐DNA vaccine treated mice compared with saline or pCAGGS‐treated mice exposed to DSS. Our results suggest that immunization with helper T epitope DNA‐vaccine targeting MIF may be a useful approach for the treatment of colitis including inflammatory bowel diseases.