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Time-resolved circular dichroism of native oat phytochrome photointermediates

Time-resolved circular dichroism (TRCD) spectral studies of the Pr→Pfr phototransformation in native oat phytochrome (124 kDa) were performed in the 500-800 nm spectral region from 500 ns to 500 ms after photolysis at 10 o C. The first intermediate observed in our measurements, lumi-R, had an absorption maximum at 695 nm, but its difference TRCD spectrum showed a CD maximum near 660 nm. This result differed from results of previous steady-state CD measurements where the intermediates of the photoconversion were trapped at low temperatures