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Laboratory Growth and Maintenance of Streptococcus pyogenes (The Group A Streptococcus, GAS)

Streptococcus pyogenes is a Gram‐positive bacterium that strictly infects humans. It is the causative agent of a broad spectrum of diseases accounting for millions of infections and at least 517,000 deaths each year worldwide. It is a nutritionally fastidious organism that ferments sugars to produce lactic acid and has strict requirements for growth. To aid in the study of this organism, this unit describes the growth and maintenance of S. pyogenes. Curr. Protoc. Microbiol. 30:9D.2.1‐9D.2.13. © 2013 by John Wiley & Sons, Inc.