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Melatonin as an antioxidant and its semi-lunar rhythm in green macroalga Ulva sp.

The presence and role of melatonin in plants are still under debate owing to difficulties of identification and quantification. Accordingly, although it has been frequently proposed that melatonin acts as an antioxidant in phototrophic organisms, experimental data on its physiological role are scarce. This study describes the use of a rapid and simple new method for quantification of melatonin in the marine macroalga Ulva sp., organisms routinely exposed to tide-related environmental stresses and known for their high tolerance to abiotic conditions. The method was used here to show that exposure to oxidative stress-inducing environmental conditions (elevated temperature and heavy metals) induced a rise in melatonin level in the algae. Addition of exogenous melatonin alleviated the algae from cadmium-induced stress. Interestingly, although the algae were taken from a culture growing free floating and kept under constant photoperiod and water level, they exhibited a semi-lunar rhythm of melatonin levels that correlated with predicted spring tides. The correlation can probably be interpreted as reflecting preparation for predicted low tides, when the algae are exposed to increasing temperature, desiccation, and salinity, all known to induce oxidative stress. Given the simplicity of the described method it can easily be adapted for the study of melatonin in many other phototrophic organisms. These results provide, for the first time, experimental data that support both an antioxidant role for melatonin and its semi-lunar rhythm in macroalgae.


Introduction
Melatonin (N-acetyl-5-methoxytryptamine; MLT) is a naturally occurring compound belonging to the indoleamines. It is involved in many biological activities in animals, among them sleep, temperature homeostasis, inhibition of parasite infection, development of tumors, and antioxidative activity (Tan et al., 2002;Rahman et al., 2005;Garcia et al., 2006;Yang et al., 2007).
Interestingly, MLT is found not only in animals but also in eukaryotic unicells, plants, algae, and prokaryotes (Reiter et al., 2001;Kö hidai et al., 2002;Hardeland and Poeggeler, 2003;Iriti et al., 2006;Paredes et al., 2009;Posmyk and Janas, 2009). Its identification in and isolation from plants and algal sources is complicated and usually requires the use of sophisticated instrumentation, such as liquid chromatography-mass spectroscopy/mass spectroscopy (LC-MS/MS) and gas chromatography/mass spectroscopy (GC/ MS), owing to the presence of similar indoles, the auxins (Cao et al., 2006). In line with its role in animal physiology, MLT in plants and algae appears to participate in the regulation of photoperiodic and rhythmic phenomena such as flowering (Kolař et al., 1997). It has been suggested that an additional important characteristic of MLT in plants and algae might be its ability to scavenge free radicals (Poeggeler et al., 2002;Tan et al., 2002;Hardeland et al., 2006;Reiter et al., 2007a). However, there is little experimental support for its function as an antioxidant in plants (Arnao and Hernandez-Ruiz, 2009a, b) and none in microalgae (Arnao and Hernandez-Ruiz, 2007;Posmyk and Janas, 2009), although numerous studies have demonstrated that MLT can scavenge radicals, both directly or by upregulating the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase, and glutathione peroxidase (Rodriguez et al., 2004;Reiter et al., 2007b). Furthermore, addition of external MLT enhances germination under chilling stress (Posmyk et al., 2008a), a known cause of oxidative stress (Prasad et al., 1995). Such studies suggested that MLT could act as a protectant against environmentally induced oxidative stress and might be beneficial for organisms facing such stress.
A multitude of marine organisms inhabit intertidal zones. Recognized as among the harshest environments on earth, such zones are characterized by rhythmic stresses of dehydration. Temperature, solar radiation, salinity, and osmotic pressure all rise and fall in a recurring rhythm as the water level changes (Denny and Wethey, 2001). These stresses are likely to be followed by oxidative stress (Kalir and Poljakoff, 1981;Wundram et al., 1996;Zue, 2000;Kakinuma et al., 2001;Abrahamsson et al., 2003), suggesting that organisms exposed to such conditions could benefit from high levels of MLT at low tide. In the present study, the protective role of MLT and the relationship between MLT level and sea level were examined in the green macroalga Ulva sp., which inhabits intertidal zones.
Ulva spp. are macroalgae that are common in intertidal coastal zone habitats worldwide (Einav, 2004), although some naturally free-floating populations have been described (Malta et al., 1999;Yabe et al., 2009). Studies employing HPLC and ELISA have shown that these macroalgae contain MLT (Toury, 2005;Pape and Luning, 2006). Here, the physiological role of MLT in an Ulva sp. was investigated by a novel quantitative method that was developed and utilized in this study. The method was based on separation of ethanolic Ulva extract by thin layer chromatography (TLC) followed by a fluorescence quantification method to measure the concentration of MLT in the algal material. This procedure was used here to study the influence of environmental stressors on MLT content and to assess the semi-lunar rhythm as a 'zeitgeber' or environmental signal for MLT content in the Ulva sp.

Materials and methods
All chemicals used were obtained from Sigma-Aldrich unless otherwise stated.

Algal material
Samples of Ulva were obtained from a culture growing free floating in an aquarium in the Israel Oceanographic and Limnological Research Center in Haifa, Israel. The cultures, which had originally been collected from rocky habitats on the eastern Mediterranean shore between Haifa and Atlit, were growing in circulating natural seawater under dimmed natural sunlight (50% shade net). The exact Ulva species was not determined, and indeed is not easy to determine (Hayden and Waaland, 2004;Loughnane et al., 2008), but the algae were from the Ulva (and not the Enteromorpha) morphological type (Hayden et al., 2003;Einav, 2004). The Ulva sp. used was probably not the previously studied Ulva lactuca, as this species is practically unknown in the Mediterranean Sea (Einav, 2004).
Algal samples were washed with distilled water to remove dirt and epiphytes. The samples were then placed in aquaria 80340350 cm and cultivated in commercial sea salt medium (dried sea salts, Aqua Medic Reef salt at a final salt concentration of 4% w/v). NH 4 Cl and NaH 2 PO 4 were added (final concentrations 1 mM and 0.1 mM, respectively) to avoid nitrogen or phosphate limitation (Israel et al., 2006). Algae were kept at a constant temperature of 23°C and under long-day photoperiod (16L:8D) with the light going on at 06:00. A 500-W halogen lamp was used as a light source. Water was constantly ventilated by aquarium air pumps and air stones. These algae were used as a stock for the experiments.

Induction of stress conditions
All experiments were carried out in triplicate. After 3 weeks cultivation algae were transferred to new aquaria and exposed to the following different potential stress inducers in illuminated growth chambers: temperature (23°C, 30°C, or 37°C); heavy metals: cadmium as CdCl 2 (Cd 2+ ; final concentrations: high, 1 mg l À1 ; medium, 0.01 mg l À1 ; low, 0); lead as PbCl 2 (Pb 2+ final concentrations: high, 5 mg l À1 ; medium, 0.05 mg l À1 ; low, 0): zinc as ZnCl 2 (Zn 2+ final concentration: high, 30 mg l À1 ; medium, 0.3 mg l À1 ; low, 0). All experiments were carried out in 2-l tanks that were ventilated as described above. When noted, exogenous MLT supplement (50 mg l À1 each time) was added together with the heavy metal and every 2 d thereafter until the end of the experiment. Each experiment lasted 7-10 d. Samples for analysis of MLT, chlorophyll, and SOD were taken as described.

Extraction of melatonin
Algal material was washed with double-distilled water and dried on a paper towel. Washed samples were ground in liquid nitrogen and the resulting powder was transferred to 1.5-ml centrifuge tubes (130 mg), each containing acid-treated glass beads (150-212 lm; Sigma), and 1.3 ml absolute ethanol. The tubes were covered with aluminum foil to avoid light-induced damage, vortexed for 30 min at room temperature, and centrifuged (10 000 g for 10 min at room temperature). Without touching the pellet, aliquots of supernatant (1 ml) were transferred to new 1.5-ml tubes and placed open in a dry block (100°C) until the solvent had completely evaporated (;45 min). The dry extract was suspended in 100 ll of ethanol (analytical grade) and kept at -20°C until used for TLC separation.
Thin-layer chromatography The TLC method described by Costantini and Paoli (1998), with some modifications, was used for MLT separation. Briefly, the resuspended ethanol extracts were loaded onto 0.2 mm thick silica gel TLC plates (737 cm, Alugram SIL G/UV254) and the plates were developed with a mixture of dichloromethane/methanol (95:5 v/v). Plates were visualized under 254 nm light (UVItec, LF-104.S, 234W) and compared with a sample pre-spiked with MLT standard to compensate for the 'matrix effect', i.e. the effect of the other extractants on the MLT retention factor. Bands corresponding to the MLT standard retention factor were scraped off with a razor blade, transferred into a new 1.5-ml tube and suspended in 1 ml of ethanol (HPLC grade). The tubes were centrifuged briefly to precipitate silica and the supernatant taken for fluorescence quantification.

Quantification of MLT by fluorescence measurement
Samples separated on the TLC plate as described above were placed in a quartz cuvette for fluorescence measurement (Fluoromax 4 fluorometer; Horiba Jobin Yvon). Samples were subjected to excitation at 280 nm and emission was recorded at 335 nm (Prozialeck et al., 1978). A calibration curve was constructed using MLT standard solution. When TLC separation was poor, the MLT standard solution was added to raise the baseline (50 ll of 0.1 ng ml À1 ). This reading was then subtracted from the peak height. Such 'standard addition' is a common practice used to solve matrix effects (Saxberg and Kowalski, 1979).
Gas chromatography/mass spectroscopy For validation of the TLC method, a TLC sample extracted as described above was analysed by GC/MS (Agilent Technologies 5975 GC/MSD) according to the protocol published by Aboul-Enein et al. (1999). N-methyl-N-trimethylsilyltrifluoroacetamide was used as a derivatizing agent, following the manufacturer's instructions (Sigma), with ethyl acetate as solvent. A similarly treated MLT standard solution was used for comparison of the results.

Protein extraction
Proteins were extracted from algal samples using the CelLytic P Cell Lysis Reagent. Briefly, powdered algae (100 mg, plus 200 ml extraction reagent in a 2-ml tube) were soaked in ice-cold water, homogenized three times (30 s each time) in a Polytron PT1200 (Kinematica), and centrifuged (10 000 g, 10 min, room temperature). The supernatant, containing soluble proteins, was frozen at -20°C until required for the activity assay.
SOD activity assay SOD activity was assayed from the protein extract using a commercial kit (SOD Assay Kit-WST; Sigma) and read with an ELISA reader (PowerWave XS; Biotek), according to the manufacturer's instructions.

Statistical analysis
Statistical analysis was carried out with SPSS. Normality tests were performed for each experiment. The influence of treatment on MLT levels, SOD activity, and chlorophyll levels was analysed by one-way ANOVA and differences between means were analysed by Student's t-test. Correlations were analysed by Pearson's correlation coefficient test.

Melatonin separation and quantification
The methods used here for MLT extraction and separation were validated by comparing the GC/MS chromatograms and m/z spectra for Ulva sp. MLT with and without MLT standard spiking. Both samples yielded clear peaks with mean retention times of 19.92 min (Fig. 1A) and characteristic ions at m/z ratios of 73, 232, 245, and 304 (data not shown; Aboul-Enein et al., 1999). Quantification by fluorescence enabled detection of MLT down to 0.5 ng MLT ml À1 (Fig. 1B).

Effect of heavy metals and the protective effect of exogenous MLT
Maximal heavy metal concentrations were chosen according to Israeli governmental regulations for wastewater sea discharge and according to Markham et al. (1980). An increase in the dosage of heavy metals had a significant positive effect on MLT levels, with the effect especially marked in the case of cadmium [r¼-0.69, P<0.05; F(2, 6)¼9.3, P<0.05] (Fig. 3A). MLT levels reached 17 ng g À1 fresh weight (FW) when the algae were exposed to 1 mg ml À1 Cd,  corresponding to an increase of 171% in MLT levels relative to levels at time zero (Fig. 3B). The effects induced by the other metals (Pb and Zn) were similar but smaller (Fig. 3A, B). SOD activity showed good correlation with MLT levels (Fig. 3C), while chlorophyll levels showed a reversed correlation, i.e. they dropped with a rise in metal concentration ( Fig. 3D; see also Kumar et al., 2010). Interestingly, addition of exogenous MLT relieved some of the oxidative stress, as evidenced by a decrease in SOD activity (Fig. 4A) and an increase in chlorophyll levels (Fig. 4B). Somewhat analogous protection by MLT was demonstrated by Posmyk et al. (2008b) and by Tan et al. (2007a, b).

Light/dark cycle
When MLT levels were determined at different times during the light/dark cycle, they were found to be maximal at night and in the early morning (02:00 and 08:00 local time). Towards early afternoon (14:00) the levels dropped, rising again to a maximum the next night. These results demonstrated a significant influence of sampling time on MLT levels [F(3,24)¼4.443, P<0.05] (Fig. 5).

Semi-lunar rhythm of MLT levels
Over a period of months, small changes in MLT levels were observed. A plot of these results against tidal lunar changes revealed a strong correlation between MLT levels and a weekly record of minimal sea level, i.e. maximum MLT levels at spring tides (r¼-0.66, P<0.01; Fig. 6).

Discussion
Identification and quantification of MLT in plants and algae is a complicated task, and accordingly its physiological roles are an issue of ongoing research. Immunoassays, for example, tend to yield high rates of false-positive results owing to the existence of many other indoles, while various other methods require the use of sophisticated and expensive equipment such as LC-MS/MS or GC/MS.
The method developed in this study offers a reliable, simple, and rapid means of extraction and quantification of MLT from the green macroalga Ulva by a TLC and fluorescence-based procedure. The identity of MLT in the expected fractions was verified by GC/MS. The quantities of MLT obtained, as well as the reproducibility of the method,   were in good agreement with published reports of phytomelatonin quantification (Murch et al., 1997;Harumi and Matsushima, 2000;Paredes et al., 2009;Posmyk and Janas, 2009). The new method was used here to quantify the levels of MLT in intertidal zone green macroalgae (Ulva sp.) under different environmental conditions. Increase in SOD activity and decrease in chlorophyll content, both well-known indicators of oxidative stress (Lagriffoul et al., 1998;Sandalio et al., 2001;Parida and Das, 2005;Kumar et al., 2010), were used to measure oxidative stress in this study.
Many organisms that inhabit intertidal zones are inevitably exposed to a combination of oxidative stress initiators (e.g. high radiation, salinity, and desiccation). Consequently, Ulva spp. are known to adapt to a wide range of abiotic oxidative stress conditions, including high temperature (these organisms can reportedly sustain temperatures from -5°C to 40°C; see Kamermans et al., 1998 andEinav, 2004), low water content (Einav, 2004), high salinity (Einav, 2004), and the presence of heavy metals (Markham et al., 1980;Villares et al., 2001), but to date no data have been published as to the mechanism underlying this capability. This study demonstrated that MLT probably participates in this adaptation, and showed that recognized sources of oxidative stress such as high temperatures (Abrahamsson et al., 2003) and heavy metals (Lagriffoul et al., 1998;Schü tzendü bel and Polle, 2002) cause a rise in MLT levels, and that exogenous MLT can relieve cadmium stress. MLT levels increased as temperatures rose from 23°C to 37°C (P<0.05) in good agreement with the known optimal photosynthesis and growth temperature for Ulva spp. (25-30°C; Kakinuma et al., 2001;Einav and Israel, 2007). Interestingly, SOD activity increased and chlorophyll content dropped only at the highest temperature (37°C) with very little change between 23°C and 30°C, suggesting that MLT provides a major, and maybe even the primary, line of defence in Ulva, probably thanks to the low costs of producing a small molecule and the MLT high radical-scavenging efficiency.
MLT levels reportedly follow a circadian rhythm in Chenopodium rubrum (Kolař et al., 1997) and in Eichhornia crassipes . MLT levels in our Ulva sample during daylight hours were lower than in the dark. This could be due to a circadian rhythm-related enzymic activation during the night as demonstrated in higher organisms, MLT disassembly pursuant to exposure to light (Andrisano et al., 1999), or increased metabolic activity during the night caused by increased rates of growth and mitotic division (Titlyanov et al., 1996;Kalita et al., 2007; also see Britz, 1976 andBritz andBriggs, 1976 for other circadian behaviour in Ulva). Interestingly, although the Ulva culture used in this study was growing free floating, it was possible to demonstrate a semi-lunar rhythm in its MLT levels. This rhythmic character fitted well with the intertidal zone habitat of the Ulva spp. At the intertidal zone sea level reach a global minimum in a reciprocal 2week cycle when the earth, moon, and sun are approximately aligned, and the tidal effects of the sun and the moon reinforce one another, resulting in spring tide. At these times radiation, desiccation, salinity, and temperature rise to a maximum leading to an increase in oxidative stress. Given the rhythmicity of this stress, it would be beneficial for a tidal zone organism such as Ulva to increase and decrease antioxidant mechanisms in advance. This study indeed showed that MLT levels follow a semi-lunar rhythm. As clearly demonstrated in the results, there was a significant negative correlation between sea levels (spring or neap tide) and MLT levels that preceded the stress-inducing changes (Fig. 6), even though the algae showing this rhythmic behaviour were not exposed to actual changes in day length or water level at the time of the experiments.
That Ulva sp. harbour semi-lunar rhythms is not a new discovery. In 1947 Gilbert Smith reported a semi-lunar rhythm when Ulva spp. release their gametes (Smith, 1947). Similar rhythms have been described in other tidal-zone organisms (Berry, 1986;Palmer, 2000;Naylor, 2001;Togashi and Cox, 2001). The correlation between MLT level and sea level might also be related to reproductive pathways, as seen in many organisms including mammals (Balik et al., 2004), birds (Ubuka et al., 2005), and plants (Paredes et al., 2009).
In summary, this study resulted in the first experimental demonstration of both the antioxidant role of MLT and its semi-lunar rhythm in macroalgae. The findings suggest that MLT might be a major antioxidant in many intertidal organisms. Arneo MB, Herná ndez-Ruiz J. 2009b. Protective effect of melatonin against chlorophyll degradation during the senescence of barley leaves. Journal of Pineal Research 46, 58-63.