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Method for establishing serum ferritin reference values depending on sex and age.

A method is described for the establishment of reference values for serum ferritin depending on sex and age. The results obtained in a stratified random sample of 353 healthy individuals from the Portuguese population revealed that 23% of the variation observed on serum ferritin values was related to sex differences and 6% of the variation was explained by age differences. Furthermore, the effect of age was different in both sexes: serum ferritin values increase with age in females over 40 years and in males under 40. The minimum and maximum reference values were established for the different subgroups of subjects as follows: for females under 40 years and males over 40, reference values were considered as the 2.5 and 97.5 percentiles from the adjusted lognormal distribution; for females over 40 years and males under 40, reference values were considered as the exponential transformation of the upper and lower limits of the 95% prediction intervals for the log of serum ferritin for each particular age point.