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Flow cytometric method for the routine follow‐up of red cell populations after bone marrow transplantation

A simple and sensitive flow cytometric method is presented for the quantitation of erythrocyte subpopulations. Cells were indirectly labelled using human antisera (mostly crude) and fluorescein isothiocyanate (FITC)‐conjugated anti‐human IgG Fab. The method was evaluated by analysing artificial mixtures of blood group antigens A, B, D, E, c, K and Fya. Intra‐assay coefficients of variation were found to be 7.2% for 1% D‐positive mixtures and 2.2% for 10% D‐positive mixtures; the inter‐assay coefficients of variation were 9.5% and 6.0% respectively. The sensitivity of the method was found to be 0.31%. The method has shown to be suitable for the routine follow‐up of patients after allogeneic bone marrow transplantation (BMT).