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SUPPLEMENTARY MATERIAL NEAR-TERM FETAL HYPOXIA-ISCHEMIA IN RABBITS: MRI CAN PREDICT MUSCLE TONE ABNORMALITIES AND DEEP BRAIN INJURY SUPPLEMENTARY METHODS Prenatal hypoxia-ischemia model. In vivo global hypoxia–ischemia of fetuses was induced by sustained uterine ischemia at 29 days gestation (90% te

In vivo global hypoxia–ischemia of fetuses was induced by sustained uterine ischemia at 29 days gestation (90% term) in timed pregnant New Zealand white rabbits (Myrtle’s Rabbits, Thompson Station, Tennessee) as described previously 1 . This scenario models acute placental insufficiency at near term gestation. Briefly, dams were anesthetized and a balloon catheter was introduced into the left femoral artery and advanced into the descending aorta to above the uterine and below the renal arteries. The balloon was inflated for 30, 32, or 40 minutes causing uterine ischemia and subsequent global fetal H-I. Sham animals underwent the same procedure but without balloon inflation. At the end of H-I, the balloon was deflated, resulting in uterine reperfusion. The catheter was then removed, femoral artery repaired, and the dams were allowed to recover. A subset of dam was imaged during H-I and reperfusion in a GE 3T clinical magnet as described earlier 2 . Four hours after H-I fetuses were delivered by laparatomy. Fetal positions in uterus were recorded to identify the position corresponding fetuses on MRI scan during H-I. Surviving kits were kept in a temperature controlled incubator and gavage fed up to E32=P1 (72 hours after H-I) with rabbit milk. At 6, 18, 24 and 72 hours after H-I newborn kits underwent neurological assessment, clinical measurement of tone and muscle tone measurements using a custom built torque-displacement apparatus and a serial MRI scan.