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Optimization of Ultrasonic-assisted Extraction of Crude Polysaccharide from Fritillary taipaiensis P. Y. Li Using Response Surface Methodology

The aim of this study was to optimize the extraction process of crude polysaccharide from Fritillary taipaiensis P. Y. Li(FTP). On the basis of single factor experiments with the method of ultrasonic-assisted extraction,extraction parameters was investigated using response surface methodology. A mathematical model including solid/liquid ratio,ultrasonic time and extraction temperature was created. By typicality analysis,the optimized extraction conditions were as follows: ultrasonic time of 16 min,extraction temperature of 75 ℃ and solid/liquid ratio of 1∶ 15(g/m L). Under the optimized conditions,the extraction yield of crude polysaccharide was determined to be 0. 461%. The results showed that the optimization process using response surface methodology was feasible. It can be used in practical forecasting.