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Study on the Relations between Hyperspectral Images of Bananas (Musa spp.) from Different Countries, Their Compositional Traits and Growing Conditions

Bananas are some of the most popular fruits around the world. However, there is limited research that explores hyperspectral imaging of bananas and its relationship with the chemical composition and growing conditions. In the study, the relations that exist between the visible near-infrared hyperspectral reflectance imaging data in the 400–1000 nm range of the bananas collected from different countries, the compositional traits and local growing conditions (altitude, temperature and rainfall) and production management (organic/conventional) were explored. The main compositional traits included moisture, starch, dietary fibre, protein, carotene content and the CIE L*a*b* colour values were also determined. The principal component analysis showed the preliminary separation of bananas from different geographical origins and production systems. The compositional and spectral data revealed positively and negatively moderate correlations (r around ±0.50, p < 0.05) between the carotene, starch content, and colour values (a*, b*) on the one hand and the wavelength ranges 405–525 nm, 615–645 nm, 885–985 nm on the other hand. Since the variation in composition and colour values were related to rainfall and temperature, the spectral information is likely also influenced by the growing conditions. The results could be useful to the industry for the improvement of banana quality and traceability.


Introduction
It has been shown that eating bananas provides health benefits in respect of hypertension, cancer, diabetes and depression [1], given their composition of several essential nutrients, such as potassium, vitamins, carotenoids, manganese, fibre and dopamine [2]. Bananas can also be easily added to a regular diet by simply eating fresh fruits or by adding them to other foods (i.e., yoghurt or smoothies). Therefore, bananas are globally frequently consumed fresh fruit. The health benefits associated with the consumption of bananas and related processed products are highly correlated with the essential nutritional contents [3,4], while the quality and composition of bananas are affected by their origin and related growing conditions.
Previous studies have demonstrated the effect of origin and related growing conditions on the composition of fruits. For example, Margraf et al. [5] reported that the geographical origin can influence the total soluble solids of Brazilian grape juices, while research on olive fruits from five different Turkish regions showed that environmental factors can potentially influence the maturity of the fruits and their phenolic fractions [6]. Different geographical parameters, including annual average  [20]; Shared according to CC BY-ND 4.0).

Geographical Data
The Global Positioning System (GPS) coordinates of the various sampling sites were provided by the local farms. The growing condition of bananas, including altitude according to GPS, monthly mean temperature and annual rainfall of harvest year were collected using Google Earth and public databases CRU TS4.04 (Climatic Research Unit (CRU) Time-Series (TS) version 4.04 of high-resolution gridded data of month-by-month variation in climate) [21] (Table 1).  [20]; Shared according to CC BY-ND 4.0).

Geographical Data
The Global Positioning System (GPS) coordinates of the various sampling sites were provided by the local farms. The growing condition of bananas, including altitude according to GPS, monthly mean temperature and annual rainfall of harvest year were collected using Google Earth and public databases CRU TS4.04 (Climatic Research Unit (CRU) Time-Series (TS) version 4.04 of high-resolution gridded data of month-by-month variation in climate) [21] (Table 1).

Hyperspectral Imaging
The schematic diagram of the hyperspectral system set is shown in Figure 2. The hyperspectral images of banana pulp and peel samples were acquired using a Specim IQ hyperspectral system (Specim, Spectral Imaging Ltd., Finland) coupled with six 50 Watt halogen lamps (Philips, The Netherlands). The Specim IQ is a portable hyperspectral camera that consists of a spectral camera (CMOS technology), a viewfinder camera (5 Mpix), a focus camera and a scanner with a motor for optics movement. The focal length of the camera is 21 mm and the effective resolution of the CMOS camera is 512 × 512 pixels. Banana pulp and peel samples were placed into a plastic container (20 × 30 cm) and then individually placed on a black plate. The distance between the samples and the camera was set to 45 cm. A white Teflon panel was initially captured and used as a white reference prior to the actual measurement. Individual sample images were acquired within 10 ms integration time in a spectral range of 400-1000 nm at 7 nm resolution in line-scan mode [22]. After the full image was scanned, raw data were recorded as separated data and corrected automatically by white and black references by the camera. The subsequent spectral extraction and multivariate data analysis were conducted by ENVI image analysis software (Harris Geospatial Solutions Inc., Broomfield, CO, USA) and MATLAB R2018b (The MathWorks Inc., Torrance, CA, USA) [23].

Hyperspectral Imaging
The schematic diagram of the hyperspectral system set is shown in Figure 2. The hyperspectral images of banana pulp and peel samples were acquired using a Specim IQ hyperspectral system (Specim, Spectral Imaging Ltd., Finland) coupled with six 50 Watt halogen lamps (Philips, The Netherlands). The Specim IQ is a portable hyperspectral camera that consists of a spectral camera (CMOS technology), a viewfinder camera (5 Mpix), a focus camera and a scanner with a motor for optics movement. The focal length of the camera is 21 mm and the effective resolution of the CMOS camera is 512 × 512 pixels. Banana pulp and peel samples were placed into a plastic container (20 × 30 cm) and then individually placed on a black plate. The distance between the samples and the camera was set to 45 cm. A white Teflon panel was initially captured and used as a white reference prior to the actual measurement. Individual sample images were acquired within 10 ms integration time in a spectral range of 400-1000 nm at 7 nm resolution in line-scan mode [22]. After the full image was scanned, raw data were recorded as separated data and corrected automatically by white and black references by the camera. The subsequent spectral extraction and multivariate data analysis were conducted by ENVI image analysis software (Harris Geospatial Solutions Inc, Colorado, USA) and MATLAB R2018b (The MathWorks Inc, California, USA) [23].   The moisture content of fresh banana pulp and peel samples were calculated by recording the weight difference before and after freeze-drying, which is expressed as g/100g (wet weight). The following compositions were expressed based on dry weight. Total starch, diet fibre contents of banana pulp and peel were determined by the Total Starch Assay Kit (AA/AMG) and Total Dietary Fibre Assay Kit (Megazyme Ltd., Ireland) [24]. The protein contents were determined by Flash EA Sensors 2020, 20, 5793 5 of 18 1112 Protein analyser (Thermo Fisher Scientific, USA) using the Dumas combustion method [25]. The carotene content was determined using high-performance liquid chromatography (HPLC) method [26]. For this HPLC method, the carotenes were extracted from the banana pulp and peel samples three times using a hexane and tetrahydrofuran solution until the pellet appeared colourless after centrifugation. The supernatant was then collected and evaporated using a vacuum evaporator. Finally, the extracted carotenes were dissolved in a sample buffer (MeOH-THF 1:1 + 0.01% BHT) and injected into the HPLC with a UV detector at 245.0 nm on an Agilent 1200 Infinity chromatograph. The mobile phase consisted of acetonitrile (A), methanol (B), ethyl acetate (C) at ratios of 60:30:10 (v:v:v) and 0.1% triethylamine. With 20 µL injection volume, samples were separated on a Phenomenex Geminin C18 Column (5 µm, 250 mm × 4.6 mm) at 30 • C with a flow rate of 1.0 mL/min. A series of standard solutions were prepared by β-carotene (Sigma-Aldrich, USA). Each sample was prepared in triplicate.

Colour (L*a*b*) Value Measurements
Colour values of the banana pulp and peel samples were determined using a Color Flex spectrophotometer (Hunter Associates Laboratory, Inc., Reston, USA) [27]. The colour meter was first standardised using a green tile, after which the banana pulp and peel powder samples were weighed (2 mg) into cuvettes, and the L*, a* and b* values measured. Each sample was measured three times to obtain an average colour reading.

Data Processing of Hyperspectral Images
As shown in Figure 2, after acquiring the images, the region of interest (ROI) was selected using the ENVI 5.3 software (Harris Geospatial Solutions Inc, America.), and the full-pixel spectra of all banana pulp and peel samples were extracted in MATLAB R2018b. The spectral value of the average spectrum of each sample was collected to generate discrimination models of geographical origin. Prior to the development of the models, spectral pre-treatment of the raw data was performed using the multiplicative scatter correction (MSC) and Savitzky-Golay (SG) methods to reduce background noise and improve spectral resolution [28]. After the spectral pre-treatment, principal component analysis (PCA) was performed for multivariate exploration of image data. Kendall correlation coefficients (r) for the spectral data, chemical composition, colour values and the growing conditions of bananas were generated in a heatmap to show their relationships by R 3.5.3 software (R Foundation for Statistical Computing, Vienna, Austria).

Data Analysis of Banana Compositions
The Shapiro-Wilk test was conducted to determine if the data was normally distributed [29]. Then, all the compositional data were further analysed and reported as mean values ± standard deviation. The significant level was determined by one-way analysis of variance (ANOVA) with Tukey's significant difference at a 5% significance level (p < 0.05). The relationship between chemical composition and growing conditions of bananas were evaluated by Kendall's correlation coefficients (r). All statistical analyses were performed using Unscrambler 10.5 (Camo Analytics, Norway) and R 3.5.3 (R Foundation for Statistical Computing, Austria) software.

Explorative Analysis of the Spectral Features, Compositional Traits of Banana Pulp, Peel and Related Growing Conditions
The spectral profiles obtained from the hyperspectral images of banana pulp and peel samples are shown in Figure 3. Most of the samples show similar reflectance values in Mean and MSC spectra. Two necessary spectral preprocessing methods (e.g., MSC and SG transformation) were used to remove certain patterns and noise among variables and to further explore spectral differences. Particularly, SG Sensors 2020, 20, 5793 6 of 18 can correct the spectrum baseline intensity caused by different particle sizes [30]. The transformed spectra of pulp and peel are shown in Figure 3. The SG transformation showed considerably more variation in spectra to reflect the spectral differences of pulp and peel samples. Furthermore, PCA was used to explore the grouping of banana pulp and peel samples from different farms based on their spectral signatures after SG transformation. The groups in the PCA plots are labelled at farm level other than the country level as several farms originated from the same countries but were cultivated with different production systems (i.e., organic and conventional methods). Therefore, the sample grouping based on geographical origin and production system can be seen in the same PCA. Differences in spectra due to the production system and growing conditions were also evident in the PCA plots ( Figure 3). DR1 and DR2 were grouped separately from Peru (PE1), under the same organic production system, in the PCA plots of the pulp and peel samples ( Figure 3). This grouping could be driven by the differences in altitude, temperature, rainfall and production systems that led to a compositional variation of starch and TDF contents which results in differences in the  The banana pulp from different farms and production systems shared similar patterns of mean spectra ( Figure 3a). However, there were differences in the position and intensity of the absorption peak. The pulp spectra had absorption peaks in the following ranges: 400-500, 640-700 and 850-920 nm. The same is evident for the peel spectra. As shown in Figure 3b, the average spectra of the peel samples in the ranges 420-490 nm and 630-720 nm reflected obvious absorption peaks. The spectral differences could be caused by different geographical origins and growing conditions. For example, Sun et al. [17] reported that all rice samples from four different regions showed characteristic absorption peaks in ranges of approximately 500-1000 nm. However, another side to consider is the evidence, as reported in recent papers, that growing conditions normally influence and cause differences in the compositional traits of foods [5]. Therefore, it is also of value to look further into the effects of growing conditions, which is specific per geographical origin, on the physicochemical composition of bananas. For the current study, the growing conditions of the banana farms are summarised in Table 1. Table 2 shows the chemical composition of the banana samples, including moisture, total starch, total dietary fibre (TDF), protein, β-carotene contents and CIE L*a*b* values.
The data distribution of chemical and colour values were shown using violin plots in Figure A1. In brief, the chemical compositions and colour values of pulp and peel samples were different according to sampling sites. The differences could be conducted the variation of local growing conditions. It is has been reported that bananas harvested from a high altitude (300 m) usually have more aroma compounds and present a firmer texture than those harvested from a lower altitude (50 m) [31]. The highest altitude (726 m) was observed in one of the farms (CR1) in Costa Rica. The lowest altitude (10 m) was reported for farm PA1 from Panama (Table 1). Correspondingly, the chemical analysis indicated that banana pulp from CR1 had significantly higher total fibre content than PA1 ( Table 2) [32]. Figure 2 shows the absorption differences in SG transformation spectra of pulp and peel in the above-mentioned wavelengths, which is linked to the starch and total dietary fibre contents (TDF) of the samples. Banana pulp and peel contained high amounts of starch and TDF (Table 2). For all the peel samples, the TDF contents are significantly higher than that of the pulp samples. This was expected as the peel is the outer, fibrous layer of the fruit, while the softer/less fibrous part is the pulp. In the current study, the total starch content of banana pulp ranged from 28.8 ± 4.4 to 70.9 ± 0.4 per 100 g DW (dry weight). When compared to the other farms, the banana pulp from PE1 had the lowest starch content at 28.8 ± 4.4% and also a lower rainfall (200 mm/year). While for TDF, the peel samples from the PA1 farm had the highest TDF content (63.6 ± 6.0%) with a higher rainfall of 3679.3 mm/year. For CO1, DR1, EC2, their TDF contents were significantly lower than PA1. These TDF findings are consistent with recent research on the composition of bananas [33,34]. These differences in composition could be due to rainfall as water is critical for the normal growth of bananas.
The values of the CIE L*a*b* colour coordinates were presented as mean ± standard deviation in Tables 2 and 3. Higher b* values correspond to samples that appear more yellow, whereas with lower a* values the samples will appear more green. Similarly, the L* is for the lightness from black (lower/negative values) to white (higher/positive values). For the colour measurement of banana pulp and peel, the L* values and b* values showed a significant difference between most farms. In the visible spectral regions (350-780 nm) of banana pulp, wavelengths of 400-500 nm and 660-705 nm showed significant absorptions related to green and orange colours, which are consistent with the banana pulp colour values of 'light yellow' on the basis of the colour meter (b* value). Absorption at 455 nm and 460 nm indicates the presence of the carotenoids [35]. As reported, bananas with yellow and orange pulp were rich in β-carotene [36]. As found in Table 2, the banana pulp samples from Farm CR2 and CR3 show increased absorption at 455 nm and 460 nm, will likely be rich in β-carotene.  In Tables 2 and 3, the content of β-carotene for pulp and peel showed significant differences between different countries, while the HSI spectral results were in line with these differences. For instance, the peel samples showed strong absorption in the 470-510 nm range, reflecting the green part of the peel, while absorption at 700 nm indicates a yellow-orange colour (b* value) for the peel. The variation in β-carotene content could be due to the growing conditions. As seen in Table 1, PA1 had the lowest monthly average temperature (19.7 • C) in terms of the average temperature of all sampling farms, whereas the highest (26.7 • C) was reported for DR1 and DR2 from the Dominican republic. A similar trend is seen for the β-carotene content of these samples, where banana pulp from PA1 had a higher β-carotene content (1.8 ± 0.2 µg/mg) than that of DR1 and DR2 (0.1 ± 0.1 µg/mg, respectively). Yang et al. [37] found that high temperatures contribute to represses chlorophyll degradation and influences the colour of banana peels. Thus, this could indicate that different growing conditions could result in differences in β-carotene contents. However, it is important to note that temperature is not the only growing condition that can influence β-carotene content. For these farms, other conditions could likely have played a role. This was consistent with other studies about fruits, which indicated that temperatures, altitudes and rainfall are correlated with secondary metabolite synthesis such as β-carotene [38,39]. Therefore, higher β-carotene content in CR2, CR3 could be caused by higher rainfall. Meantime, the high β-carotene content of PE1 could be due to organic production used in Peru, Bunea et al. [40] reported that organic agriculture resulted in higher ß-carotene concentration in grape. However, there are also papers illustrating no evidence of nutritional superiority of the organically grown fruits [38]. Yet, for the current study, there is not enough evidence to prove this effect and more research is needed for its verification. As for the β-carotene content in the peel samples, Colombia, Costa Rica, Peru and Panama had significantly higher contents compared to those from the Dominican Republic and Ecuador ( Table 3). The content differences in β-carotene according to geographical origins were similar to results reported for other food products, such as acerola cherry [38], jujube fruit [39] and milk [41].
Differences in spectra due to the production system and growing conditions were also evident in the PCA plots ( Figure 3). DR1 and DR2 were grouped separately from Peru (PE1), under the same organic production system, in the PCA plots of the pulp and peel samples (Figure 3). This grouping could be driven by the differences in altitude, temperature, rainfall and production systems that led to a compositional variation of starch and TDF contents which results in differences in the characteristic spectral values. The two farms from Ecuador, EC1 (organic) and EC2 (conventional), also practiced different ways of cultivation, and grouped separately in the PCA plot of the pulp and peel spectra ( Figure 3); further indicating that the production system could potentially be indicated by HSI. In light of this, Su and Sun [42] found that the HSI conducted with PCA could easily separate organically and conventionally planted potatoes. Table 2 shows that EC1 and EC2 pulp samples had significant differences in TDF content. At the same time, the difference in rainfall of these two farms was also quite notably different; EC1 with 1511 mm/year and EC 2 with 843 mm/year. A markable absorption around 995 nm that is likely related to the aromatic amines in pulp and peel spectral was identified, indicating that bananas often contain volatile substances and unique flavours [43]. For other compositional traits such as moisture and protein content, it is easy to recognise that banana peel has more moisture than pulp (~89% vs.~74%, respectively) because the total starch content accounts for a large proportion of banana pulp. The pulp samples from DR1 had the highest moisture content (75.3 ± 5.9%), which is significantly higher than banana pulp samples from CR1, EC1 and EC2. Nguyen et al. [44] found the moisture content (on a wet basis) to be 74.7 ± 1.3% for banana pulp from Australia, which reflects the similar values of the current study. For the moisture content of peel samples, the samples from the Dominican Republic (DR2) had the highest value, while the lowest content was reported in Ecuador (EC2). However, there are no significant differences between different groups, which means growing conditions did not have a great influence on the moisture content of bananas. Lei et al. [45] reported that bending vibration around 700 nm (N-H) was attributed to protein. In Figure 3, the variation of HSI spectra around 700 nm could be caused by different protein contents of pulp and peel. Generally, the banana peel samples have higher protein contents than the pulp samples (~5-6% vs.~3-4%, respectively). PCA plots also showed that in comparison with the protein content of the pulp samples, the results revealed that banana peel had higher protein contents, such as 6.8 ± 0.7% for CR2. However, only a few farms showed significant differences in pulp and peel samples concerning the protein contents from all sampling countries. The DR1 bananas had considerably higher protein contents than bananas from other farms for pulp and reflected remarkable differences comparing peel samples from farm CR2 (p < 0.05). Table 1 showed that DR1 had quite a higher altitude, which could be the underlying mechanism. Mohapatra et al. also reported that the different content of banana protein could be caused by genomic mutation, altitude and climate [46].
Not only the effects of geographical factors but also the effects of production systems were reflected in PCA plots. As seen in Figure 2, three organic farms (DR1, DR2 and EC1), were separated from other conventional farms both based on HSI spectra of pulp and peel, which proved that the effects of different production systems can be observed in the hyperspectral results. In further, compositional traits from Table 2 also showed that pulp from organic cultivation had more contents of Moisture, starch and total dietary fibre and less β-carotene significantly (p < 0.05) in comparison with conventional methods. The notably higher starch content also could be found in peel samples (p < 0.05). However, one of the organic farms, namely PE1, can not be separated from other conventional farms. This suggested that the difference in the HSI spectrum is due to the combined action of growing conditions. Lima et al. [47] also pointed out that organic agriculture has great potential to increase the content of certain nutrients in food, but, obviously, there are more factors such as crop time, climate, soil characteristics, environmental conditions still make contributions.
Previous studies usually used the PCA plots to show the grouping of samples based on their different geographic locations [5,28]. However, our research shows that the potential differences in spectra are not only caused by geographical origin, but are also very much related to the characteristic growth conditions such as altitude, temperature and rainfall. Even for the bananas collected from the neighboring countries such as Colombia and Panama, the PCA plots showed that CO1 grouped separately from PA1 according to the pulps' spectral fingerprint as both farms have large differences in altitude and rainfall. For the peel samples, although the DR1 and EC1 farms had a quite long geographical distance between them, they were still grouped closely. This could be because both of them shared similar temperature and rainfall, and were cultivated with a similar organic system. In view of this, Sun et al. [35] also demonstrated that HSI conducted with PCA could address the difference between two shrimp groups from high-and low-salinity environments.

Correlation of the HSI Spectra, Compositional Traits Colour Data and Growing Conditions
In the last ten years, HSI technology has been widely used in food authenticity and fraud research, especially adulteration, agricultural product traceability, organic food identification and other related fields [48]. However, most articles mainly tend to use hyperspectral techniques to accurately identify sample differences, but few works of literature report the causes of these differences. In the above parts of this paper, the chemical compositions and value databases from banana pulp and peel samples were obtained by a series of approaches. The growing conditions and HSI spectral data were acquired as well. To explore how HSI spectra relate to the differences in compositional traits and growing conditions, the spectral data were correlated with the compositional data, colour values and growing conditions (Figure 4). Figure 4a shows the correlation between the HSI spectra (Savitzky-Golay transformation) on the X axis and the compositional, colour, growing conditions on the Y axis. The correlation analysis was based on the characteristic peaks caused by certain compositions and colours of pulp and peel under HSI wavelengths 400-1000 nm [28]. For the wavelength ranges 405-525 nm, 615-645 nm and 885-985 nm, a relatively strong correlation was observed. The HSI spectra of the banana pulp samples (Figure 4a) also showed higher differences in these ranges according to different growing conditions. Compared with protein, TDF, moisture, the carotene and starch contents showed a stronger correlation with HSI spectra in the 405-525 nm and 615-625 nm wavelength ranges. As the most abundant component of banana pulp, the moderate positive correlation (r = 0.39-0.45, p < 0.05) of starch with HSI indicates the potential of HSI to identify starch-rich foods. As reported in Section 3.1, absorptions at 876 nm and 967 nm are generally related to C-H group of fibre and O-H stretching vibration of starch [49], therefore moderate correlations (r values around 0.40, p < 0.05) of starch of pulp also were shown in the 885-985 nm range. Compared to banana pulp, weaker correlations of most of the traits and HSI exist for the peel sample. This is possibly due to the fact that banana pulp is more likely to reflect the influence of growing conditions on the composition and colour values. At the same time, the spectra of the banana peel and the PCA results also showed that the difference between the banana peels is smaller than those for the pulps. Ultimately, HSI could reveal the difference in the composition of bananas and is a suitable tool to link the compositional traits to the growing conditions of bananas.

Correlation between Growing Conditions and Banana Composition
Research studies on bananas have mainly reported the relationships between the climate and yield of bananas, and few papers have focused on the correlation of local growing conditions and banana compositional traits [9]. The contents of chemical compositions of plants the results of absorption, utilization and metabolic activities [50]. The starch content of sweet potatoes was increased in high altitudes farms [51]. The effects of different altitudes and temperatures on moisture content, reducing sugars, crude fibre as well as color were studied in yacon tuberous roots [52]. However, the correlations of growing conditions and food compositions were still scarcely reported in the literature. To understand the effects of growing conditions on the composition and colour

Correlation between Growing Conditions and Banana Composition
Research studies on bananas have mainly reported the relationships between the climate and yield of bananas, and few papers have focused on the correlation of local growing conditions and banana compositional traits [9]. The contents of chemical compositions of plants the results of absorption, utilization and metabolic activities [50]. The starch content of sweet potatoes was increased in high altitudes farms [51]. The effects of different altitudes and temperatures on moisture content, reducing sugars, crude fibre as well as color were studied in yacon tuberous roots [52]. However, the correlations of growing conditions and food compositions were still scarcely reported in the literature. To understand the effects of growing conditions on the composition and colour variation of the bananas, correlations between those datasets were investigated by Kendall's correlation coefficients (r). In Figure 5, The coefficients and the significant correlations are shown from red (negative) to blue (positive) (p < 0.05), while the insignificant correlations are marked as blank. There are few publications reporting on correlations between the growing condition of banana and the composition. It was reported that the harvest season of the banana field will affect the firmness of the banana fruit [10]. Apart from this, some research studies reported that a quite strong correlation (r = 0.62-0.94) was noted between rainfall levels and fruit firmness [53]. In the review, the cited research showed that the relationship of climate differences and fruit quality, the vitamin content, phenolic compound content and micronutrients of fruits are highly affected by temperature change, water availability and other geographical variables [9]. from red (negative) to blue (positive) (p < 0.05), while the insignificant correlations are marked as blank. There are few publications reporting on correlations between the growing condition of banana and the composition. It was reported that the harvest season of the banana field will affect the firmness of the banana fruit [10]. Apart from this, some research studies reported that a quite strong correlation (r = 0.62-0.94) was noted between rainfall levels and fruit firmness [53]. In the review, the cited research showed that the relationship of climate differences and fruit quality, the vitamin content, phenolic compound content and micronutrients of fruits are highly affected by temperature change, water availability and other geographical variables [9]. For the pulp samples, mostly positive correlations were found between temperature, rainfall, chemical composition and colour values (Figure 5a). The colour index a* had a moderate and slightly positive correlation with temperature (r = 0.32, p < 0.05), while the L* factor correlated negatively with temperature. For the former, the positive correlation means that with increasing temperature an increase in a* values (more red and less green colour) can be expected. This finding coincides with the fact that a higher temperature is helpful to the growth of bananas and promotes the pulp to change from white to light yellow. This also explains why the L* value is positively correlated with temperature. Because L* stands for lightness of samples, the lightness of pulp will be decreased in the colour transformation during growth. Growing conditions such as rainfall resulted in a moderate positive correlation (r = 0.47, p < 0.05) with carotene content for the banana pulp. Therefore, higher carotene contents can be expected for bananas grown in regions with more extensive rainfall. In our research, the banana pulp from CR2 had the highest carotene content, also the highest annual rainfall is reported on this farm as well. Most of the chemical and colour values were only slightly influenced by altitude, indicating that the effects of temperature and rainfall were higher than those of altitude. For the pulp samples, mostly positive correlations were found between temperature, rainfall, chemical composition and colour values (Figure 5a). The colour index a* had a moderate and slightly positive correlation with temperature (r = 0.32, p < 0.05), while the L* factor correlated negatively with temperature. For the former, the positive correlation means that with increasing temperature an increase in a* values (more red and less green colour) can be expected. This finding coincides with the fact that a higher temperature is helpful to the growth of bananas and promotes the pulp to change from white to light yellow. This also explains why the L* value is positively correlated with temperature. Because L* stands for lightness of samples, the lightness of pulp will be decreased in the colour transformation during growth. Growing conditions such as rainfall resulted in a moderate positive correlation (r = 0.47, p < 0.05) with carotene content for the banana pulp. Therefore, higher carotene contents can be expected for bananas grown in regions with more extensive rainfall. In our research, the banana pulp from CR2 had the highest carotene content, also the highest annual rainfall is reported on this farm as well. Most of the chemical and colour values were only slightly influenced by altitude, indicating that the effects of temperature and rainfall were higher than those of altitude.
In contrast to the pulp samples, temperature largely showed significant negative correlations with most of the chemical components and colour values of the peel samples. The temperature had a slight negative correlation with the protein content of peel samples (r = −0.18, p < 0.05), which could be due to the effect of temperature on banana metabolism. Similar correlations were determined for the protein contents of salmon fillets. Ørnholt-Johansson et al. [49] reported that a negative relationship exists between the protein content of salmon and sea temperature.
Particularly, the temperature had a negative correlation with carotene content (r = −0.16, p < 0.05), which is consistent with the findings in carrot research [54]. As we found in Section 3.1, the banana pulp farm PA1 within high temperature showed higher β-carotene content than that of DR1 and DR2 within lower temperatures. A similar study about the influences of temperature on bananas colour also reported that high temperature could decrease the yellow colour of bananas [37]. A negative correlation between temperature and b* value (r = −0.39, p < 0.05) was observed, whereas the b* value reflects the yellow colour of banana peel. A moderate positive correlation was observed between rainfall and the b* colour value (r = 0.35, p < 0.05); hence, higher rainfall could enhance the banana peel colour as an increase in b* value will result in a more intense yellow colour of the peel. As reported in Section 3.1, farm PE1 with the lowest rainfall showed the lowest b* value compared with other farms (p < 0.05). From Figure 5b, the same correlation trends were observed between the results of carotene content and colour indexes of peel samples. The impact of growing conditions on the fruit's compositions is due to the effect of temperature and rainfall affecting the metabolism of nutrients in fruits [55].
These results demonstrate that different growing conditions had certain effects on the chemical compositions and colour values of banana pulp and peel samples. Climatic factors, such as annual average precipitation, relative humidity and average temperature, have also been reported to have either positive or negative effects on the metabolites of Flos Carthami flowers [56]. The different altitudes were also reported having influences on organic acids and aroma volatile attributes of pomegranate fruit [57]. Similarly, CIE L*a*b* colour spaces are useful for the identification of honey types from different geographical locations [58]. When discussing food authenticity, it is vital to consider the growing conditions as food quality, flavour and economic value often benefit from special geographical conditions.

Conclusions
The results demonstrate that banana pulp and peel from different geographical origins and production systems have unique spectral fingerprints that can be linked to their chemical composition and associated growing conditions. PCA revealed that the spectral range from 400-1000 nm is an effective range to show the differences in geographical origin and production system of bananas using a portable HSI system. The combined effects of rainfall, temperature and altitude are likely some of the main causes for the variation in the carotene, starch and dietary fibre contents. The colour values are more related to altitude and temperature at the geographical locations. The wavelength ranges 405-525 nm, 615-645 nm and 885-985 nm of HSI were significantly correlated (r values around ± 0.50, p < 0.05) with the carotene content, starch content, and a* and b* colour values. Indirectly, the differences in HSI spectral data could reflect the effects of rainfall, temperatures and altitudes (growing conditions) on compositional traits of bananas. These findings help to better understand the effects of growing conditions on the colour differences and the variation in the composition of bananas.