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Testing for a suitable culture medium for micropropagation of East african higland bananas

A study was conducted to find a suitable culture medium for
micropropagation of East African highland bananas ( Musa spp.).
Modified Eriksson (ER), Gamborg’s B-S (B-S) and Murashige-Skoog
(MS) media were tested with a roasting cultivar Gonja-Horn plantain
(AAB), a dessert Bogoya-Gros Michel (AAA) and three East African
highland cooking (AAA) cultivars Kibuzi, Mbwazirume and Namwezi for in
vitro proliferation induction. The media were supplemented with 4.5 mg
l -1 Benzylaminopurine (BAP) and with or without 0.186 mg l -1
Naphtalene acetic acid (NAA). Only ER and MS gave proliferation. AAB
cultivar proliferated significantly more than AAAs which did not differ
among themselves. The AAA cultivars showed precocious rooting in the
absence and presence of 0.186 mg l -1 NAA on ER and MS media
supplemented with 4.5 mg l -1 BAP. However, the presence of auxin NAA
significantly lowered shoot proliferation with concomitant significant
induction of precocious rooting. This indicated a high level of
endogenous auxin in these cultivars and, therefore, the unnecessity of
exogenous auxin for their maximum in vitro proliferation. It was also
found that ammonical nitrogen content determined the suitability of a
culture medium thus making ER an alternative to the MS salts.