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Assessment of Genetic Diversity of an Endangered Species Fraxinus hupehensis Based on ISSR Markers

Investigation on the level and pattern of genetic diversity of 10 natural populations of the endangered species Fraxinus hupehensis using inter-simple sequence repeat (ISSR) markers was crucial for understanding the structure of the population and assessing the best genetic protection strategies. A total of 180 polymorphic bands with the polymorphic rate of 100.00% were amplified by 14 primers. The genetic diversity at population level (Percentage of polymorphic loci, PPL=64.06; Nei’s gene diversity index, h=0.1519; Shannon’s information index, I=0.2434) was lower than that at species level (PPL= 100.00%, h=0.1833, I=0.3041). Analysis of molecular variance (AMOVA) demonstrated the low level of the genetic variation occurred between the populations (16.05%). This also can be corroborated by the gene flow (Nm 2.424) and the coefficient of gene differentiation (Gst=0.1710) among populations. Cluster analysis based on the unweighted pair group method with arithmetic averages (UPGMA) revealed four groups for 10 populations according to Nei’s genetic identity and seven categories for the 196 individuals according to SM values. Furthermore, the endangered mechanism and genetic structure of F. hupehensis were discussed, and appropriate targeted protection measures were proposed based on these findings.