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Inhibition of malignant potential and expression of proteins associated with epithelial-mesenchymal transition in Lewis lung carcinoma cells transduced with murine ifn-β gene in recombinant baculovirus.

AIM
To analyze biological characteristics, malignant potential and expression of proteins associated with epithelial-mesenchymal transition in murine lung carcinoma cells transduced with interferon-beta (ifn-β) gene in baculovirus vector.


MATERIALS AND METHODS
The study was performed on Lewis lung carcinoma (LL) cells transduced with ifn-β gene in recombinant baculovirus vector. Biological characteristics of the LL cells were studied with the use of standard cell culture methods, cytogenetic and immunocytochemical assays.


RESULTS
Recombinant baculovirus-mediated transduction of LL cells with ifn-β gene resulted in significant decrease of cell growth rate and density both in complete and serum-free medium. Also, LL cells transduction with ifn-β gene significantly inhibited cell migration in vitro. Transduction of LL cells by baculovirus vector with or without ifn-β gene caused significant genotoxic effect in these cells. Furthermore, ifn-β gene transfer to lung carcinoma cells resulted in significant increase of nuclear expression of p19(ARF) (p < 0.01), p21(WAF1) (p < 0.01), cytoplasmic expression of E-cadherin (p < 0.005) and inhibition of transcription factors of epithelial-mesenchymal transition (EMT) Twist (p < 0.005) and Slug (p < 0.01) expression.


CONCLUSIONS
Transduction with ifn-β gene of LL cells in recombinant baculovirus resulted in acquirement of less malignant phenotype in vitro and suppressed expression of proteins associated with EMT.