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HPTLC Fingerprinting Profile and GC-MS Analysis of Aqueous extract of Alstonia scholaris Linn. Bark

Aim: The research was aimed at evaluating HPTLC finger print analysis and GCMS analysis of the plant Alstonia scholaris bark extract. Materials and Methods: The powdered material was subjected to maceration with distilled water for about 48hrs. Then the extract collected was evaporated to dryness under reduced pressure and subjected to HPTLC and GC MS analysis using standard protocol. Results: In HPTLC, the black spot present in TLC plate indicated the presence of glycosides. Absence of orange spot in the TLC plate derivatized with Dragendorff’s Reagent indicated the absence of alkaloids. GC MS analysis showed the presence of 27 compounds matching their recorded spectra with the data bank mass spectra of NIST library V 11 provided by the instruments software. These results indicated that the Alstonia scholaris bark extract contain various bioactive components with wide range of medicinal properties, justifying the use of this plant to treat various ailments.