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New method for hydrogen isotope analysis of non-structural carbohydrates

Analysing stable isotope composition of biologic components can be a powerful tool to reconstruct past environmental conditions, physiological responses, and to trace metabolic pathways. The analysis of the carbon-bound non-exchangeable hydrogen isotope ratios (δ 2 H NE ) in carbohydrates can be challenging, partly due to the exchangeability of oxygen-bound hydrogen in the same molecule with those in water or vapour. To eliminate such sample alterations, carbohydrates have been nitrated to substitute exchangeable hydrogen with nitrate ester. However, the nitration of carbohydrates is time consuming, needs high sample amount, has several safety issues, and the nitrated products of short-chained carbohydrates are instable. δ 2 H NE