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Apheresis products of the Amicus™ and the AS.TEC 204® cell separators are comparable with regard to dendritic cells derived from the mononuclear cell collection

Background  In this study, we investigated the quality of autologous mononuclear cells (MNC) collected with two different cell separators using standard MNC‐apheresis procedure modalities. MNCs were purified by density gradient centrifugation and cultured according to standard protocols to generate dendritic cells (DC) and 1 × 107/ml immature DCs were pulsed with tumour lysate for 3 days and subsequently characterized by fluorescent‐activated cell sorter analysis.