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Study on the differentially expressed genes of traumatic human cerebral cortex by genechip

Objective To analyze the difference of gene expression profile between the traumatic and normal human brain, and look for new molecular biological strategies for preventing and treating brain trauma. Methods Five traumatic cerebral cortex tissue, which came from operative patient suffering from tramatic brain injury (TBI) within 2 days, were collected. The total RNA of traumatic tissue was extracted respectively in Trozal reagent, and normal total RNA of human cerebral cortex came from Ambion company. By using human Oligo genechip containing 21 329 genes, the differentially expressed genes were detected between 5 traumatic brain tissues and 1 normal brain tissues. 2 genes expressing differentially were randomly selected to be validated by quantitative real-time PCR( qRT-PCR). Results There were 87 common genes in those differentially expressed genes from trauma brains comparing with the normal brain, 60 genes were up-regulated and 27 were down-regulated. Among the up-regulated genes, 44 genes were categorised into 12 functional categies, the other 16 genes function were unknown or ESTs. Among the down-regulated genes, 7 genes were be categorised 4 functional categies, the other 20 genes function were unknown or ESTs. Those functional genes were searched in Pubmed, and cd44、ccl2、tieg、vegf、cdl4、xbpl、egrl、rgs2、fos、il-l b、timp1、gadd45a,cdknla、cnn3、sppl were once reported in literatures on brain trauma. qRT-PCR confirmed the reliability of the chip data. Conclusions The most of the 87 commonly differentially expressed genes had not been reported in previous brain trauma literature, including bag3、cyr61、clicl、plscrl、gbel. New molecular biological strategies dealing with brain trauma will be developed with intervening them.