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Separation of inosine, hypoxanthine, and guanosine by high-performance liquid chromatography on silica.

The separation of inosine (Ino), hypoxanthine (Hyp), and guanosine (Guo) on silica has been studied. In adsorption normal-phase systems the peak shapes are unsatisfactory; a low selectivity has been observed for the Ino-Hyp and Guo-Hyp pairs. Chromatograms can be significantly improved if systems with a mixed partition-adsorption retention mechanism are applied.