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Development of a Bioimpedance Measuring Plate for the Study of Hypoxic and Hyperoxic Conditions in Cell Cultures

The oxygen plays an important role in the development and function of the human brain. In the central nervous system, a different neuron types can be very sensitive to varying concentrations of oxygen. Despite several cell biology researches, the time course of the effects of non-physiological oxygen concentrations, but especially high oxygen concentrations on the membrane of neurons, and the dynamics of damage are unknown. In the present study, with this in view, electrical impedance spectroscopy (EIS) measurements are carried out using a graphene-based self-developed four-electrode measurement and data collection process. Our goal was to produce a plate layout for our further experiments that could be used to examine cell cultures under hypoxic and hyperoxic conditions. The designs differ in the distance between the electrodes, we used three types of distances: 1 mm, 3 mm 5 mm, using one distance per plate. On the plate with 5 mm electrode spacing we found the ideal. From a measurement point of view, the plate with 5 mm electrode spacing was the most suitable. As we increased the distance, the size of the electrode surfaces decreased. It can be assumed that reducing the size of the electrodes did not negatively affect the measurement results. Our results can greatly contribute to better understand the process of cell membrane damage.