{'fraction_non_alphanumeric': 0.021396396396396396, 'fraction_numerical': 0.0, 'mean_word_length': 5.442028985507246, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
In this issue

To rest or not to rest Flow cytometry is routinely used in clinical immunomonitoring. For the comparison of cell samples taken at different time points, cells might be cryopreserved and analyzed at once thereafter. Due to marker down-regulation and cell dysfunction by cryopreservation, flow cytometry based assays for cryopreserved peripheral blood mononuclear cells (cPBMCs) face a challenge. Wang and coworkers established, therefore, a standardized resting process concerning position, cell concentration, resting period and material of cell culture tubes as well as culture media. Since resting of cPBMCs is not helpful for the flow cytometric analysis of myeloid-derived suppressor cells (MDSCs) and large/intermediate size lymphocytes, they developed an algorithm to indicate for which cell population and which type of analysis the resting process is useful or not.