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Wild Honey Facilitates Antibacterial Efficacy of Penicillin and Amoxicillin-Clavulanic Acid.

Background: Antibacterial efficacy of honey has been well acknowledged from ancient to modern society. Utilizing a wild honey, the present strategy of the study was chosen to address an unfilled demand for finding a complement of penicillin analogues which have already been reported resistant in many cases. 
 
Methods: In this study, penicillin and amoxiclav were trialed against four clinically resistant bacteria  two gram-positives (Staphylococcus aureus and Streptococcus pyogenes) and two gram-negatives (Klebsiella pneumoniae and Acinetobacter baumannii). Afterwards, these bacteria were exposed to different concentrations of a natural wild honey. Finally, the honey was used in conjugation with penicillin and amoxiclav individually. Zone of inhibition, minimum inhibitory and bactericidal concentrations were observed in this regard. 
 
Results: The wild honey showed great potential to inhibit bacterial growth at its lowest dose given (6.25%) both against Staphylococcus (48%) and Streptococcus species (45%) but could not exhibit any bactericidal effect alone. However, it greatly influenced both penicillin (100%) against Acinetobacter and amoxiclav (93%) against Klebsiella species whereas these two antibiotics could not demonstrate good antibacterial effect when applied individually against these bacteria. 
 
Conclusion: All findings suggested that wild honey not only possess inhibitory effect but also Facilitates other agents action, thereby, generating a scope for research for identifying new potential antibacterial agents.