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Bio-Assemblies onto Conducting Polymer Support: Implementation of DNA-Chips

The electrical deposition(electropolymerization)of polypyrrole, PPy, grafted by different ODN probes is the key to construct oligonucleotides, ODN, arrays on silicon devices bearing a matrix of gold microelectrodes. The straightforward functionalization of PPy allows finalizing a more general grafting based on affinity interactions between a biotinylated polypyrrole support and the biological probe and results in a surface renewable DNA sensor. The electrical monitoring of the thickness of the PPy deposit at the nanometer range allows the real time monitoring of the biological interactions using Surface Plasmon Resonance imaging. Recently, the electroconductivity of the PPy was exploited to trigger the electrochemiluminescence of a DNA sequence labelled with a luminol derivative, ABEI.