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TopoisomeraseⅡα Gene for Prognostic Prediction of Hepatocellular Carcinoma: A Bioinformatics Analysis.

OBJECTIVE
To investigate the expression of topoisomeraseⅡα (TOP2α) in hepatocellular carcinoma (HCC) and its role in predicting prognosis of HCC patients.


METHODS
We used HCC-related datasets in UALCAN, HCCDB, cBioPortal and Enrichr databases to analyze the expression and mutation of TOP2α and its co-expressed genes in HCC. GO function and KEGG pathway enrichment of TOP2α and its co-expressed genes was identified. The TIMER database was used to analyze the levels of infiltrated immune cells in HCC. The impacts of TOP2α and its co-expressed genes and the infiltrated immune cells on the survival of HCC patients were assayed by Kaplan-Meier plotter analysis.


RESULTS
TOP2α and its co-expressed genes were highly expressed in HCC (P < 0.001) and detrimental to overall survival in HCC patients (P < 0.001). TOP2α and its co-expression genes were mainly involved in cell mitosis and proliferation, and cell cycle pathway (ID: hsa04110, P = 0.001945). TOP2α and its co-expressed genes were mutated in HCC and significantly detrimental to overall survival (P = 0.0247) and disease-free survival (P =0.0265) in HCC patients. TOP2α was positively correlated with infiltration of B cell (r = 0.459, P < 0.01), CD8+T cell (r = 0.312, P < 0.01), CD4+T cell (r = 0.37, P < 0.01), macrophage (r = 0.459, P < 0.01), neutrophil(r = 0.405, P < 0.01), and dendritic cell (r = 0.473, P < 0.01) in HCC. The high levels of CD8+ T cell infiltration significantly prolonged the 3 and 5 years survival of HCC patients (all P < 0.05), and the high levels of CD4+ T cell infiltration significantly shortened the 3, 5, and 10 years survival of HCC patients (all P < 0.05).


CONCLUSION
TOP2α may be an oncogene, which was associated with poor prognosis of HCC patients and could be used as a biomarker for the prognosis of HCC.