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[The frozen bovine RPE cells release a resolvable material which can promote the proliferation of RPE cells].

3H-thymidine incorporation (as a proliferation maker) confirms that the frozen bovine RPE cells release a resolvable material which can promote the proliferation of RPE cells, and the molecular weight of the material is more than 10,000 Daltons. This result provided some new explanations about the effect and complications of ophthalmic cryotherapy related to RPE.