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In vitro anticancer efficacy of a polyphenol combination of curcumin, quercetin, green tea extract and resveratrol in human cervical cancer HeLa cells

Cervical cancer is the third most commonly diagnosed cancer and fourth leading cause of mortality in women worldwide. Although fully treatable in the early stages, once it has metastasized, the survival rate is 68%. Based on its poor prognosis, we investigated the effect of a specific polyphenol combination (PB) of curcumin, quercetin, green tea extract and resveratrol on viability, invasion, MMPs secretion and morphology on human cervical cancer HeLa cells. Cervix HeLa cells (ATCC) were cultured in DEM media, supplemented with bovine serum and antibiotics in 24‐well tissue plates. At near confluence, cells were treated with PB at 0, 10, 25, 50, 75 and 100 μg/ml concentration in triplicate at each dose. Cells were also treated with PMA for MMP‐9 induction. Cell proliferation was evaluated by MTT assay, MMPs by gelatinase zymography, invasion by Matrigel and morphology by H&E staining. PB inhibited proliferation by 10, 25, 30, 30 and 60% at 10, 25, 50, 75 and 100 μg/ml concentration. Zymography demonstrated expression of MMP‐2 and MMP‐9, with strong induction of MMP‐9 by PMA. Both MMPs were reduced by PB with total block at 50 μg/ml concentration. PB inhibited invasion through Matrigel at 25 μg/ml concentration. H&E staining showed no morphological changes up to 50 μg/ml concentration and exhibited apoptotic changes at 75 and 100 μg/ml concentrations. These results suggest that PB has promising new therapeutic potential in treatment of cervical cancer.