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Nucleotide sequence of a developmentally regulated Dictyostelium discoideum endogenous plasmid gene and 5' flanking region.

We have sequenced a region encoding the development- specific gene D5 (1) from the endogenous plasmid Ddpl of Dictyostelium discoideum. The nucleotide sequence predicts a hydrophilic protein of 22.7kd with 35 charged residues, 27 of which are basic. There is no apparent signal sequence or membrane-spanning domain. We have suggested elsewhere (2) that the function of the development-specific transcripts may be as repressors of replication initiation. A soluble, basic protein could have such a function. The first in-phase Met not immediately followed by a termination codon is taken as the start site of translation. Note, however, that the sequences immediately preceding this Met differ from the usual D.discoideum poly (A) stretch. By Kozak's rules (3) the G at position -3 would suggest a low level of translation of the mRNA. The 5' flanking region shows characteristic D.discoideum upstream elements (underlined). These include a consensus D.discoideum TATA-like box followed by an oligo (dT) stretch.A G-rich stretch upstream from the TATA box has striking homology with sequences in similar positions upstream from developmentally-regulated chromosomal D.discoideum genes (4).