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A fine method of evaluating the physiological function of vestibular hair cells

We have established a fine method of evaluating the physiological function of vestibular hair cells. We examined the mechano-electrical transduction (MET) to study the physiological function of a hair cell. Hair cells composing the vestibular maculae were loaded with fura-2 AM, and the Ca2+ responses to the mechanical stimulation of hair bundles were monitored. The vestibular maculae were dissected from 1 to 3-day-old rats and were incubated with fura-2 AM for 30 min. Next, the Ca2+ responses generated through the activation of the MET were studied by the water puff stimulation of hair bundles. In addition, the acute effect of gentamicin was confirmed by this method.