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In vitro progesterone stimulates the release of luteinizing hormone-releasing hormone from superfused hypothalamic tissue from ovariectomized estradiol-primed prepuberal rats.

This study was designed to determine if in vitro treatment with progesterone (P4) would activate the LHRH apparatus in hypothalmic fragments from ovariectomized, estradiol- primed rats. Immature, 28-day-old female rats were ovariectomized and implanted with Silastic capsules containing estradiol. At 30 days of age, the animals were decapitated, and the mediobasal hypothalamic-anterior hypothalamic-preoptic area fragments were removed and transferred to supervision chambers. These hypothalamic units received P4, delivered either in pulses (10 min on, 20 min off; 10 ng/ml) or in a continuous mode. LHRH was determined in perfusates and postsuperfusion tissues by RIA. The intermittent mode of P4 administration unequivocally stimulated episodic LHRH release. A maximal stimulatory effect of P4 (close to a 4-fold increment over pretreatment levels) was observed about 1 h after initial P4 administration. After a decline in LHRH release to approximately initial levels, it took about 1 h more for the intermittent P...