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Histochemical localization of some hydroxysteroid dehydrogenases in the mouse epididymis.

The mouse epididymis was studied to localize histochemically a number of hydroxysteroid dehydrogenases in the various zones. The epithelium of the posterior half of the initial segment (head) and the anterior half of the middle segment (body) shows a strong reaction for delta5-3beta-, 3alpha,5alpha-, 3alpha,5beta-, 11beta, 16alpha-, 17beta, 20alpha-hydroxysteroid dehydrogenases. This activity attenuates posteriorly. Only the 11beta-hydroxysteroid dehydrogenase is present throughout the length of the epididymis. The luminal contents of the middle segment also show the histochemical utilization of a number of steroids.