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Monocyte Chemoattractant Protein-1 and C-C Chemokine Receptor Type 2 Expression related to Body Temperature Changes

mechanism shown to regulate iNOS expression. Therefore, this study examined the relationship between LPS-induced iNOS mRNA expression and indices of pro-inflammation, oxidative stress, and apoptosis in isolated leukocytes of aerobically fit (AF) and unfit (UF) males. METHODS: iNOS mRNA expression and TNF-α, MDA, and p53 concentrations were quantified from 3-hour LPS stimulated and unstimulated whole blood. RESULTS: iNOS mRNA expression remained unaltered following LPS stimulation in AF and UF subjects (p = 0.146). However, LPS stimulation significantly lowered MDA concentrations to a greater extent in UF compared to AF subjects (p = 0.001), whereas LPS stimulation increased TNF-α and lowered p53 to a similar extent in both groups (p = 0.002, p= 0.022, respectively). Interestingly, change in relative iNOS mRNA expression was not associated with the percent change (control vs. LPS stimulation) in the concentrations of MDA, TNF-α, and p53. CONCLUSIONS: Findings suggest that although aerobic fitness did not alter iNOS mRNA expression following LPS stimulation and may not directly impact indices of pro-inflammation or the pro-apoptotic marker p53 in healthy, young males, fitness may impact LPS-induced oxidative stress.