preS1 of HBV-pre6
α Helix 46-50
Hepatocyte receptor binding
Hepatitis B Virus
Kyou-Hoon Han
preS1 of HBV
Pre-structured motifs in the natively unstructured preS1 surface antigen of hepatitis B virus

Seung-Wook Chi, Do-Hyoung Kim, Si-Hyung Lee, Iksoo Chang, and Kyou-Hoon Han

The preS1 surface antigen of hepatitis B virus (HBV) is known to play an important role in the initial attachment of HBV to hepatocytes. We have characterized structural features of the full-length preS1 using heteronuclear NMR methods and discovered that this 119-residue protein is inherently unstructured without a unique tertiary structure under a nondenaturing condition. Yet, combination of various NMR parameters shows that the preS1 contains “pre-structured” domains broadly covering its functional domains. The most prominent domain is formed by residues 27–45 and overlaps with the putative hepatocyte-binding domain (HBD) encompassing residues 21–47, within which two well-defined pre-structured motifs, formed by Pro32–Ala36 and Pro41–Phe45 are found. Additional, somewhat less prominent, pre-structured motifs are also formed by residues 11–18, 22–25, 37–40, and 46–50. Overall results suggest that the preS1 is a natively unstructured protein (NUP) whose N-terminal 50 residues, populated with multiple pre-structured motifs, contribute critically to hepatocyte binding.