[User Manual] PathHunter® β-Arrestin2 Retroparticles

[User Manual] PathHunter® β-Arrestin2 Retroparticles

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The PathHunter β-Arrestin2 Retroparticles includes Moloney murine leukemia retrovirus (MMLV) expressing β-arrestin2 fused to the β-galactosidase (β-gal) reporter fragment called EA (enzyme acceptor). The Retroparticles can be used for effective transduction of a wide variety of dividing target cells (e.g. CHO, HEK 293, U2OS) resulting in high-level expression of the encoded β-Arrestin2-EA fusion protein. This product is intended for use with a plasmid or virus expressing a ProLink™-tagged GPCR (or any β-arrestin binding protein) to create assays that report on β-arrestin binding to a target protein of interest or ligand-induced β-arrestin2 recruitment to the cytoplasmic tail of the GPCR of interest. When using this product, we highly recommend that users possess a working knowledge of virus handling and tissue culture techniques.