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24-Hour Urine Collection

What is a 24-hour urine collection?

A 24-hour urine collection is a simple lab test that measures what’s in your urine. The test is used to check kidney function. A 24-hour urine collection is done by collecting your urine in a special container over a full 24-hour period. The container must be kept cool until the urine is returned to the lab.

Urine is made up of water and dissolved chemicals, such as sodium and potassium. It also contains urea. This is made when protein breaks down. And it contains creatinine, which is formed from muscle breakdown. Normally, urine contains certain amounts of these waste products. It may be a sign of a certain disease or condition if these amounts are not within a normal range. Or if other substances are present.

Why might I need a 24-hour urine collection?


Conditions that can cause kidney disease include:

  • Diabetic nephropathy.  This happens when someone has uncontrolled diabetes. It causes high levels of protein (albumin) in the urine and can lead to kidney damage.

  • High blood pressure.  Abnormally high blood pressure can lead to lasting (permanent) kidney damage.

  • Lupus nephritis. Lupus is an autoimmune disease where the immune system attacks the kidneys and damages them.

  • Frequent urinary tract infections.

  • Prolonged urinary tract blockage.

  • Alport syndrome.  This health problem causes vision and hearing problems, as well as progressive scarring of the kidneys. The syndrome is passed down through families.

  • Nephrotic syndrome. This health problem has several different causes. Symptoms include protein in the urine, low protein in the blood, high cholesterol levels, and tissue swelling.

  • Polycystic kidney disease.  This health problem causes the growth of many fluid-filled cysts in the kidneys. This makes the kidneys larger. Over time, it takes over and destroys working kidney tissue.


  • Screening for preeclampsia in pregnancy.  Preeclampsia is a dangerous health problem that sometimes occurs in pregnancy. It causes high blood pressure and can lead to organ failure. 

  • Kidney stones (nephrolithiasis). Kidney stones form because of an imbalance of minerals, salts, and water in the urine.

There may be other reasons for your healthcare provider to advise 24-hour urine collection.

What are the risks of a 24-hour urine collection?

A 24-hour urine collection is a safe, easy test. People can collect urine on their own.


  • Forgetting to collect some of your urine


  • Losing urine from the specimen container through spilling

  • Not keeping urine cold while collecting it

  • Acute stress

  • Vigorous exercise

  • Certain foods, such as coffee, tea, cocoa, bananas, citrus fruits, and vanilla

There may be other risks depending on your specific health problems. Discuss any concerns with your healthcare provider before the collection.



  • Make sure you understand if you need to stay away from certain foods while collecting your urine.

  • You will be given large containers to store your urine and a container to urinate into. Make sure you know how to use them. Have a cold place to store the urine while you’re collecting it. For instance, a refrigerator or in a cooler on ice.

  • You may be told to start the collection at a certain time.

  • If possible, choose a 24-hour period when you will be at home so you don't have to transport your urine.


  • Make sure your provider has a list of all medicines (prescription and over-the-counter), herbs, vitamins, and supplements that you are taking.


What happens during a 24-hour urine collection?

A 24-hour urine collection may be done on an outpatient basis. This means you go home the same day. Or it may be done during a hospital stay. Procedures may vary depending on your condition and your healthcare provider's practices.

Generally, a 24-hour urine collection follows this process:

  1. You will be given 1 or more containers for collecting and storing your urine. A brown plastic container is typically used. A special pan that fits in the toilet or a urinal may be used to collect the urine. You will need to transfer the urine from the collecting container to the storage container. You will need to keep it cold.

  2. The 24-hour collection may start at any time during the day after you urinate. But your healthcare provider may tell you when to start. It is common to start the collection the first thing in the morning. It is important to collect all urine in the following 24-hour period.

  3. Don't save the urine from your first time urinating. Flush this first specimen, but note the time. This is the start time of the 24-hour collection.

  4. All urine, after the first flushed specimen, must be saved, stored, and kept cold. This means keeping it either on ice or in a refrigerator for the next 24 hours.

  5. Try to urinate again at the same time, 24 hours after the start time, to finish the collection process. If you can’t urinate at this time, it is OK.

  6. Once the urine collection has been completed, the urine containers need to be taken to the lab as soon as possible. If you are doing the urine collection at home, you will be given instructions on how and where to take it.

  7. Depending on your specific health problem, you may be asked to repeat the collection over several days.

What happens after a 24-hour urine collection?

There is no special type of care after a 24-hour urine collection. But your healthcare provider may give you other instructions after the procedure, depending on your particular situation.

Next steps


  • The name of the test or procedure

  • The reason you are having the test or procedure

  • What results to expect and what they mean

  • The risks and benefits of the test or procedure

  • What the possible side effects or complications are

  • When and where you are to have the test or procedure



  • Any alternative tests or procedures to think about

  • When and how will you get the results



Online Medical Reviewer: Marc Greenstein MD
Online Medical Reviewer: Raymond Kent Turley BSN MSN RN
Online Medical Reviewer: Raymond Turley Jr PA-C
Date Last Reviewed: 2/1/2021
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