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In vitro Evaluation of Efficacy of Non-starch Polysaccharides Enzymes on Wheat by Simulating the Avian Digestive Tract.

In this study, the efficacy of different non-starch polysaccharide (NSP) enzyme sources on wheat ingredient and wheat basal diets in vitro was evaluated by simulating the avian digestive tract. In Exp. 1, pH level was increased from 2.0 to 8.0 by simulating the avian digestive tract. The relative enzyme activities of xylanase A, B and C and β-glucanase X at pH 3.0-3.5 were higher (P < 0.05) than those at pH 2.0 or 7.0-8.0. The optimal pH levels of 3.5 and 7.0 were screened by simulating the proventriculus and small intestine, respectively to evaluate the efficacy of NSP enzyme on wheat sources. In Exp. 2, wheat 1 contained the highest content of NSP fractions and the lowest digestibility in vitro dry matter (IVDMD) and energy (IVED) in wheat samples. Therefore, wheat 1 was selected for hydrolysis research under different NSP enzyme sources and levels (1500, 4500, 13500, 40500, 121500 U xylanase/kg and 250, 500, 1000, 2000, 4000 U β-glucanase/kg) in vitro. The hydrolysis of wheat on basis of the released reducing sugar content was determined by xylanase sources A > B > C (P < 0.05) and β-glucanase sources of X > Y (P < 0.05). On basis of the hydrolysis, the optimum dose of xylanase A and β-glucanase X were 40500 U /kg and 2000 U /kg, respectively. Subsequently, the completely randomized designs involving two NSP enzymes treatments × two endogenous digestive enzymes treatments (Exp. 3), as well as two wheat basal diets × two NSP enzymes treatments (Exp. 4) were used to evaluate the efficacy of NSP enzymes on dietary nutrient digestibility. The addition of NSP enzymes (40500 U xylanase A/kg and 2000 U β-glucanase X/kg) increased the IVDMD and IVED of wheat 1 without endogenous enzymes (P < 0.05), while the IVDMD and IVED of wheat 1 with endogenous enzyme were only slightly increased (P > 0.05). The addition of NSP enzymes could increase the IVDMD and IVED of corn-wheat-soybean meal diet (P < 0.05), but had no effect on those of wheat-cottonseed meal rapeseed meal diet (P > 0.05). In conclusion, xylanase and β-glucanase additions could effectively eliminate the adverse effects on wheat and wheat basal diets at the optimal pH levels of 3.5 and 7.0 by simulating the proventriculus and small intestine parts, respectively. The efficacy of NSP enzymes was influenced by the enzyme sources, dietary type, and the interaction of endogenous enzymes.