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Toxicogenomic difference between diethylstilbestrol and 17β‐estradiol in mouse testicular gene expression by neonatal exposure

In this study, we investigated the effects of neonatal exposure to exogenous estrogen (diethylstilbestrol: DES, 17β‐estradiol: E2) on testicular gene expressions. Male C57BL/6J mice, 1 day after birth, were subcutaneously injected with DES or E2 (3 μg/mouse/day) for 5 days, and then they were raised for 8 weeks. In morphological observation of 8‐week‐old mice testes, spermatozoa were absent from many seminiferous tubules in DES‐treated mice testes, but there was no change in E2‐treated mice testes. Analysis of in‐house cDNA microarray (mouse cDNA 889 genes) revealed that 17 genes were altered in DES‐treated mice testes at 8 weeks of age, compared to each control. Real‐time reverse transcription‐polymerase chain reaction (real‐time RT‐PCR) analysis of these genes revealed that some genes, which were changed in E2‐treated testis, were the same as in DES‐treated testis, whereas in other cases there was a difference between DES‐treated and E2‐treated testis. The present results suggest that each exogenous estrogenic compound has both a common gene expression change pattern and its own testicular gene expression change pattern. Mol. Reprod. Dev. 67: 19–25, 2004. © 2004 Wiley‐Liss, Inc.