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Cytological diagnosis of herpesvirus keratitis by means of in situ hybridization: report of a case.

Typical herpesvirus keratitis that developed in a 12-year-old boy was initially diagnosed cytologically by the Papanicolaou method demonstrating the 'ground-glass' appearance of the nuclei with multinucleated syncytial cells. Subsequently, the in situ hybridization technique was applied to identify the herpes simplex virus (HSV) DNA in the Papanicolaoudestained cellular samples. Positive hybridization was found with intense staining for the HSV DNA in the nuclei of cells having a 'ground-glass' appearance. In situ hybridization has been shown to be a useful technique for the identification of HSV in corneal scrapes, and similar studies may be carried out in cellular samples from the other body sites.