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IMMUNOPROTECTIVE EFFECT OF MELATONIN ADMINISTRATION ON METHYLPREDNISOLONE-TREATED WISTAR MALE RAT (RATTUS NORVEGICUS)

Background: Immune system function decreases with age. Steroid use might accelerate this process, especially in adults in Indonesia. Melatonin is a natural hormone which show antioxidant effect and immunoprotective effect. Melatonin administration might help in reviving humane immune system due to aging and exogenous steroid exposure. 
Objective: This experiment is aimed to determine the effect of melatonin on leukocyte and lymphocyte count of methylprednisolone-induced immunosuppression on Wistar rat. 
Method: This study was true experimental with post-test only control group design using 32 male rats. Rats were divided into control and the intervention group. Control group was given methylprednisolone 0.144 mg/200 g rat 3 times a day and distilled water. The treatment group was given methylprednisolone 0.144/200 g rat 3 times a day and 0.054 mg/200 g melatonin once daily. The blood was taken after 14 days of treatment. 
Results: Intervention group shows higher lymphocytes count (4.95 ±1.58x103/L) compared to the control group (3.091.33x103/L), with p < 0.01. The intervention group also has higher leukocyte count (8.37± 2.02x103/L) compared to the control group (6.70 ± 1.96x103/L), with p<0.05. 
Conclusion: This study concluded that oral melatonin inhibited the decrease number of lymphocytes and leukocytes in rats given methylprednisolone.