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NUDT1: A potential independent predictor for the prognosis of patients with oral squamous cell carcinoma.

OBJECTIVE
The current study was aimed to investigate the association of Nudix Hydrolase 1 (NUDT1) levels with the prognosis of oral squamous cell carcinoma (OSCC) patients.


MATERIAL AND METHODS
Western immunoblotting and QRT-PCR were used to detect the protein and mRNA levels of NUDT1 in 31 cases of OSCC and normal tissues. The paraffin-embedded 62 cases of OSCC and 18 normal tissues were collected and the pathological alterations were assessed by immunohistochemistry. The prognosis of all patients were followed up. Kaplan-Meier method was used to analyze the survival rate, and Cox regression was used for multivariate analysis.


RESULTS
Both the protein and gene levels of NUDT1 were statistically increased (P = 0.0007 and P < 0.0001) in the OSCC tissue and had a significant association with the histopathologic grades of OSCC (P < 0.0001 and P = 0.0223). Immunohistochemistry detection of NUDT1 in 62 human OSCC tissues and 18 normal control tissues showed that NUDT1 expression was significantly increased in OSCC tissue and showed a strong association with histopathologic grades (P < 0.0001) and tumor stage (P = 0.005). Patients with high NUDT1 expression exhibited poorer overall survival rate (OS) and tumor-specific survival rate (TSS) than those with low NUDT1 expression (P < 0.0001 and P = 0.0008), NUDT1 was independent prognostic factors for OS and TSS (P < 0.0001 and P < 0.001).


CONCLUSION
The expression level of NUDT1 might be used to predict the prognosis of OSCC patients.