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Quantitation of fetal hemoglobin by densitometry.

Abstract Densitometry of hemoglobins separated by cellulose acetate electrophoresis has become a routine procedure for quantitating hemoglobins in hospital and hemoglobinopathy screening laboratories. Although the alkali denaturation technique is the analytic method of choice for the quantitation of fetal hemoglobin (Hb F), many laboratories have been using densitometry to report Hb F levels. To evaluate the accuracy of densitometry for Hb F quantitation, 31 Hb AF samples and 16 Hb SF samples were separated by cellulose acetate electrophoresis. Densitometric Hb F levels were compared with the levels determined by the Singer alkali denaturation test. Densitometry was found to be unsatisfactory for quantitation of Hb F.