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New compact‐type latex photometric immunoassay system for hemoglobin and three acute inflammation markers: Neutrophil count, C‐reactive protein, and anti‐streptolysin O

A new compact‐type latex photometric immunoassay system, SPOTCHEM IM SI‐3510 (ARKRAY, Inc., Kyoto, Japan), which assays three kinds of inflammatory markers—neutrophil count (NPC), C‐reactive protein (CRP), and anti‐streptolysin O (ASO)—was evaluated. Hemoglobin (Hb), which is a good marker for anemia, can also be measured with it. NPC and CRP are measured using antibodies against neutrophilic elastase and CRP, purified streptolysin O was used for ASO determination, and Hb was measured by an azide‐methemoglobin method. Whole blood, serum, and plasma specimens can be used as samples with this system. In this study, whole blood treated with dipotassium ethylenediamine tetraacetic acid was used for evaluation. Linearity and reproducibility were good for all of the items studied. Good correlations were observed between the results obtained by this system and those obtained by routine methods. Since NPC exhibited a high correlation with the routine white blood cell (WBC) counts, it was judged to be useful as a substitute for WBC counting. Since this system is small and easy to operate, and evaluation revealed reliable results, it was judged to be practical for small laboratories, and satellite testing in hospitals and physicians’ office laboratories for patients suspected to have acute inflammation. J. Clin. Lab. Anal. 16:95–102, 2002. © 2002 Wiley‐Liss, Inc.