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Dependence of transmembrane voltage on pulsed electric field frequency

This paper deduced the relationship of transmembrane voltage on cell membrane and frequency by solving the Laplace's equation. The value of transmembrane voltage will decrease when the frequency of outer electric field increases, and this decrease will be faster if the frequency exceeds a certain frequency band. The transmembrane voltage is determined by frequency components contained in the pulse on the condition of the electric field intensity is fixed when a pulsed electric field is applied. The more low-frequency components contained, the larger transmembrane voltage is, and thus a better sterilization effect can be obtained. By comparing the frequency components contained in the three types of pulses mostly used in PEF and their sterilization effect respectively, the analysis of relationship of transmembrane voltage-frequency is validated.