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Use of CMOS Image Sensor for early detection of ischemic and haemorrhagic stroke

We present the development of a lab-on-chip system potentially able to determine specific miRNA levels that enable a differential diagnosis between ischemic and hemorrhagic stroke, through the specialization of CMOS Image Sensors. In particular, the system allows investigations on the photoluminescence of samples of biological liquid to be analyzed (plasma, lysate, biological fluid) following the capture of the specific miRNA by an antisense set of ad hoc designed Peptide Nucleic Acids (PNA) that confers the biological specificity and sensitivity. The CMOS Image Sensor-biochip is modified with a first PNA that captures the target miRNA. A second PNA bringing a fluorescent tag binds the target miRNA enabling detection of the 3-component complex by the CMOS.