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[Effects of laser stimulation with different power on oxygen consumption rate of C2C12 myoblasts in mice].

OBJECTIVE
To investigate the effects of 650 nm laser irradiation on cell oxygen consumption rate in C2C12 myoblasts following different doses.


METHODS
C2C12 cells were irradiated with 650 nm laser(λ=650 nm, p=5 mW) with energy densities of 0, 0.4, and 0.8 J/cm2. Cell oxidative function was measured by oxygen consumption rate kit. Protein expression of myogenic determination factor (MyoD), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), mammalian target of rapamycin (mTOR) and its phosphorylation were detected by Western blot.


RESULTS
Compared to the control group, the expression levels of MyoD, PGC-1α protein were increased and cell oxygen consumption rate was promoted in the low dose group(P<0.05). MyoD and PGC-1α protein expressions were also increased(P<0.05), the ratio of mTOR and its phosphorylationwere decreased significantly in the high dose group(P<0.05).


CONCLUSIONS
650 nm laser irradiation that dose is 0.4 J/cm2 enhances cell oxidative function, it related to that proper dose laser irradiation promoted the expression of PGC-1a protein.