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Diversity of Symbiodiniaceae in 15 Coral Species From the Southern South China Sea: Potential Relationship With Coral Thermal Adaptability

It is well-known that the adaptability of coral-Symbiodiniaceae symbiosis to thermal stress varies among coral species, but the cause and/or mechanism behind it are not well-understood. In this study, we aimed to explore this issue based on zooxanthellae density (ZD) and Symbiodiniaceae genus/subclade. Hemocytometry and next-generation sequencing of the internal transcribed spacer region 2 (ITS2) marker gene were used to observe ZDs and Symbiodiniaceae genera/subclades associated with 15 typical coral species in the southern South China Sea (SCS). Average ZDs of all corals were in low levels, ranging from 0.84 to 1.22 × 106 cells cm−2, with a total of five Symbiodiniaceae genera, Symbiodinium, Cladocopium, Durusdinium, Fugacium, and Gerakladium, as well as 24 dominant subclades, were detected and varied among these coral species. Pocillopora verrucosa was dominated by Durusdinium (subclade D1/D1a), and other colonial corals were dominated by Cladocopium, but the subclades were varied among these species. Porites lutea and Montipora efflorescens were dominated by C15, and Echinopora lamellosa, Hydnophora exesa, and Coscinaraea exesa were dominated by C40. Acropora corymbosa, Merulina ampliata, and five species of Faviidae were mainly associated with Cladocopium types of C3u and Cspc. In contrast to other colonial corals, the dominant subclade of solitary Fungia fungites was C27, with high host specificity. Our study indicates that coral thermal stress adaptability is mainly affected by dominant Symbiodiniaceae type instead of ZD in the southern SCS. Some heat-sensitive corals, such as P. verrucosa corals, have acquired a high abundance of heat-tolerant Durusdinium to adapt to thermal stress. This could be the main reason for these corals becoming the dominant corals in this reef region. Background subclades analyses showed significant differences among coral species in subclade quantity and diversity. These suggest that numbers of coral species may have adapted to high environmental temperature by adopting various symbionts and/or associating with heat-tolerant Symbiodiniaceae.


INTRODUCTION
Coral reef ecosystems, with high biodiversity and productivity, are facing severe threats from anthropogenic disturbance and global warming (Brown, 1997;Bellwood et al., 2004;Yu, 2012). Reef corals rely on their symbiotic relationship with Symbiodiniaceae (i.e., zooxanthellae), which provide ∼95% of their energy and enable them to develop in oligotrophic tropical seas (Falkowski et al., 1984). Abnormally elevated sea surface temperatures (SSTs) disrupt this symbiosis, resulting in zooxanthellae discharge and coral death (Baker et al., 2004(Baker et al., , 2008. Since the 1990s, coral reefs around the world have frequently experienced global-scale bleaching events, mainly including the 1998, 2010, and 2015/2016 global thermal bleaching events (e.g., Hoegh-Guldberg, 1999;Loya et al., 2001;Hughes et al., 2017Hughes et al., , 2018. The 1998 thermal bleaching event caused great damage to global coral reefs. More than 16% of global coral reefs were lost−87% of corals in the inshore reefs of the Great Barrier Reef were bleached, ∼95% of corals died after bleaching in Bahrain and the Maldives, and ∼85% of corals were bleached and lost in Japan (Wilkinson, 1998;Berkelmans and Oliver, 1999;Loya et al., 2001). Record high temperatures during 2015/2016 triggered the third recorded global bleaching event since the first large-scale bleaching in the 1980s, causing severe harm to the survival and health of global coral reefs (Hughes et al., 2017(Hughes et al., , 2018. Experimental and in situ studies have found that corals' tolerance to thermal stress varies widely among species (Marshall and Baird, 2000;Abrego et al., 2008;Wicks et al., 2010;Wooldridge, 2014). In natural conditions, branching Acropora corals with low zooxanthellae densities (ZDs) are vulnerable to thermal bleaching, while massive Favia and Porites corals with high ZDs have higher tolerance to thermal stress (Li et al., 2008(Li et al., , 2011. Branching Acropora and Pocillopora corals are the dominant colonial species in the tropical coral reefs of the Indo-Pacific and are considered to be highly vulnerable to thermal bleaching (Loya et al., 2001;Wooldridge, 2014). In 1998, these species suffered severely from a bleaching event and a large number died (Berkelmans and Oliver, 1999;Marshall and Baird, 2000;Loya et al., 2001). Since that time, subsequent global bleaching events have occurred (Moore et al., 2012;Hughes et al., 2017;Nohaïc et al., 2017). Surprisingly, a number of coral genera/species (e.g., Porites and Montastraea corals) survive after repeated thermal stress, indicating that these corals may have begun to develop increased thermal adaptability (Pratchett et al., 2013;Silverstein et al., 2015;Boulotte et al., 2016;Guest et al., 2016). Previous studies have shown that zooxanthellae density is an important indicator of coral susceptibility to thermal stress (Marshall and Baird, 2000;Li et al., 2008;Wooldridge, 2014;Xu et al., 2017). However, due to limited reports on its diversity and abundance, it is difficult to assess whether density is a factor affecting its potential for adaptation to climate change. Further research into intraspecific variations in coral tolerance and adaptability to thermal stress can help to shed light on this question.
Investigating the diversity and function of Symbiodiniaceae offers insight into their response to thermal stress (LaJeunesse et al., 2003;Baker et al., 2004;Jones et al., 2008). Recent applications of novel techniques such as quantitative PCR and NGS have yielded increasing information on previously unknown low-abundance Symbiodiniaceae genera and subclades (Bay et al., 2016;Ziegler et al., 2017Ziegler et al., , 2018. In coral symbiotic microbial ecosystems, rare biospheres represent low-abundance and highly diverse clades accounting for <1%, which are generally referred to as background types (Thomas et al., 2014;Ziegler et al., 2017). Background Symbiodiniaceae subclades are important for coral-symbionts' adaptation to thermal stress (Boulotte et al., 2016;Lee et al., 2016;Ziegler et al., 2018). Coral hosts under such stress might accommodate Symbiodiniaceae by either symbiosis "reorganization" or "transformation" (Fautin and Buddemeier, 2004;Apprill and Gates, 2010;Cunning et al., 2016). Some coral species, such as Pocillopora damicornis and Stylophora pistillata, have been shown to resist and/or recover from thermal stress by altering the relative abundance of Symbiodiniaceae types (Baker et al., 2004;Silverstein et al., 2015;Boulotte et al., 2016;Ziegler et al., 2018). However, the role of background Symbiodiniaceae in dealing with thermal stress is insufficiently understood and requires further exploration, particularly of their composition and diversity.
The Spratly Islands (i.e., Nansha Islands, 3 • 35 ′ -11 • 55 ′ N, 109 • 30 ′ -117 • 50 ′ E) are located in the southernmost area of the South China Sea (SCS), with typical tropical reefs near the equator (Yu, 2012). The area has high diversities of scleractinian corals with high genetic diversity and connectivity, which are associated with a variety of microorganisms (Zhao et al., 2013;Liang et al., 2017;Huang et al., 2018). Previous macro-ecological surveys in the central and southern SCS have found that massive Porites and Montipora dominate, with additional prevalence of heat-sensitive branching Pocillopora corals (Zhao et al., 2013(Zhao et al., , 2016. They are affected by abnormal high temperature at times, and numerous of corals bleached under thermal stress (Yu and Zhao, 2006;Li et al., 2011;Yu, 2012). In this study, two fundamental issues were addressed: (i) we currently do not know the density, diversity, and composition of Symbiodiniaceae in numerous coral species and the main influence environmental factors in the southern SCS, and (ii) under the frequent threat of thermal bleaching events in recent decades, why the branching Pocillopora corals, which are generally considered to be heatsensitive, are still dominant in the southern SCS? We tested the hypotheses that: (i) there would be clear differences in ZDs and Symbiodiniaceae genera among coral species in the southern SCS that would be shaped by local environmental factors, and (ii) the branching Pocillopora would have high ZDs or be associated with heat-tolerant Symbiodiniaceae genera to enhance their resistance to thermal stress and to be dominant in this coral reef region (CRR). To explore these questionsthe ZDs and Symbiodiniaceae genera/subclades of 15 typical scleractinian coral species-local water quality parameters were collected at Xinyi Reef of the Spratly Islands. This study identifies the ZDs and Symbiodiniaceae genera/subclades among fifteen coral species and the main affecting factors. The results indicate variation in the ZDs and Symbiodiniaceae genera/subclades, and the potential relationship with bleaching susceptibility to the local environment stresses among coral species. Our findings contribute to further understanding the variation in adaptation to thermal stress among coral species under global warming.

Study Site and Sampling of Corals
Our study site was located at Xinyi Reef (9 • 20 ′ 6 ′′ N, 115 • 55 ′ 49 ′′ E) of the Spratly Islands, southern SCS ( Figure S1). This is a small tropical atoll (∼6.8 km 2 ), and corals are mainly distributed on reef flats and slopes. Mean annual SST is relatively high at 28.7 • C, with a monthly range from 27.4 • C in January to 31.7 • C in June ( Figure S2). SSTs were obtained from satellite-derived datasets of NASA, Ocean Color Radiometry, monthly averaged MODIS-Aqua 9 km, from January 1997 to December 2015 (https://giovanni.gsfc.nasa.gov). Seawater temperature, salinity and transparency were measured during sampling. Dissolved oxygen (DO) was assessed in situ using a DO200A portable meter (YSI Inc., Yellow Springs, OH, USA). Seawater samples were collected and immediately filtered (Whatman GF/F; GE Healthcare, Chicago, IL, USA). Dissolved inorganic nitrogen (DIN) and soluble reactive phosphorus (SRP) were measured using a continuous flow analyzer (SEAL QuAAtro; SEAL Analytical Shanghai, Shanghai, China). Forty-eight coral specimens from eight families and 15 genera, including Pocillopora verrucosa, Acropora corymbosa, Montipora efflorescens, Pavona varians, Echinopora lamellosa, Merulina ampliata, Porites lutea, Favia palauensis, Favites abdita, Goniastrea aspera, Diploastrea heliopora, Platygyra daedalea, Hydnophora exesa, Coscinaraea exesa, and Fungia fungites, were randomly collected in three to five replicates (∼30 cm 2 each sample) at 2-6 m water depth (Table S1). These species contained four morphologies, i.e., branching corals, massive corals, plating corals, and solitary corals. The distance between replicates was above 10 m, and in situ photography was carried out at the same time to verify species identification. Each specimen was divided into two portions, where one (∼25 cm 2 ) was used to determine ZD and the other (∼5 cm 2 ) was used for Symbiodiniaceae DNA extraction. All specimens were preserved at 0-4 • C and immediately transported to the laboratory.

Determination of Zooxanthellae Density
Coral tissue was removed using a Waterpik TM (3-5 kgf cm −2 ) containing seawater (passed through a 0.45 µm filter) until only white coral skeleton remained (1-3 min). At this point, the entire tissue was considered to be completely removed. To reduce the error as far as possible, all the experiments with ZDs were conducted by the same experimenter. Initial slurry volume was measured in a graduated cylinder. The slurry was then homogenized and subsampled into four 3-mL aliquots, then centrifuged at 4000 rpm for 5 min. ZDs were calculated using replicate hemocytometer counts (n = 8). Surface area was determined based on the correlation between aluminum foil weight and surface area (Marsh, 1970;Fitt et al., 2000;Li et al., 2008).

DNA Extraction, PCR Amplification and Illumine MiSeq Sequencing
Coral samples of ∼50 mg, including tissue, mucus, and skeleton, were used to extract genomic DNA using DNeasy R Plant Mini Kit (Qiagen, Hilden, Germany) following the manufacturer's protocol provided with the kit. After quality and purity examinations, the extracted DNA samples were applied as PCR templates. The Symbiodiniaceae ITS2 region of rDNA was amplified by PCR using primers F: 5 ′ -GAATTGCAGAACTCCGTG-3 ′ and R: 5 ′ -GGGATCCATATGCTTAAGTTCAGCGGGT-3 ′ , with a six-nucleotide barcode unique to each sample (LaJeunesse and Trench, 2000;LaJeunesse et al., 2003). PCR amplifications were conducted in a 50 µL reaction volume containing ∼50 ng of DNA, 25 µL of 2X Taq Platinum PCR Master (Tiangen, China), 200 nM of each primer, and ddH 2 O to make up the final volume. Reactions were performed at 94 • C for 5 min, followed by 35 cycles of 94 • C for 30 s, 51 • C for 30 s, and 72 • C for 30 s, and a final extension at 72 • C for 5 min using a ABI GeneAmp R 9700 thermocycler as described by Sun et al. (2014). Triplicate PCR products were pooled for each sample, and fragments with size in range of 301 to 340 bp were purified and quantified using the AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City, CA, United States) and QuantiFluorTM-ST Fluorescence quantitative system (Promega, United States). Purified amplicons were pooled in equimolar amounts and paired-end sequenced (2 × 250) on an Illumina MiSeq platform according to standard protocols (Majorbio Bio-Pharm Technology Co. Ltd., Shanghai, China). Raw reads were deposited into the NCBI Sequence Read Archive (SRA) database (Accession Number: SRP 186860).

Next-Generation Sequencing Data Processing and Data Analysis
Strict quality control and sequence filtration were applied to ensure analytical accuracy. Adaptors, short reads, and lowquality reads were removed by the sequencing company. A paired-end read merger (PEAR) tool was applied to obtain full-length ITS2 rDNA fragments with merging overlapping PE reads to generate ITS2 sequences . ITS2 tags were de-multiplexed into all samples in the QIIME platform by identifying unique barcodes (Caporaso et al., 2010). A BLAST Symbiodiniaceae-specific database of ITS2 types was downloaded (Supplementary Materials ITS2 Database.fasta) (Franklin et al., 2012;Arif et al., 2014;Chen et al., 2019). Sequences were assigned to the ITS2 types that gave the highest identity in the BLAST hits (Altschul et al., 1990). To evaluate Symbiodiniaceae diversity and community composition, ITS2 sequence data alignment analysis and OTU analysis were used in this study (Arif et al., 2014;Tong et al., 2017;Ziegler et al., 2017). In addition, dominant/sub-dominant Symbiodiniaceae genera/subclades (≥1%) were analyzed both by ITS2 sequence data alignment analysis and OTU analysis, and the background subclades (<1%) were analyzed by ITS2 sequence data alignment analysis (Arif et al., 2014;Tong et al., 2017;Ziegler et al., 2017). The resulting counts of Symbiodiniaceae ITS2 types were merged for downstream statistical analysis.
Inter-species variations in ZDs were investigated using oneway ANOVA after the assumptions testing of homogeneity, normality, and independence. The Student-Newman-Keuls (SNK) test was used for post hoc multiple comparisons of significant ANOVA results. All ZDs data are presented as means ± standard deviations (SD). Shannon's diversity index (H'), which is based on the OTU framework, was calculated to assess the level of alpha-diversity across samples in the Vegan package by R (v. 3.1.2) software environment (Dixon, 2003;R Core Team, 2014). The difference of Symbiodiniaceae diversity and community composition among the 15 coral species was conducted by Wilcoxon rank-sum test. The similarity of Symbiodiniaceae assemblages was also characterized using non-metric multidimensional scaling (nMDS) using the Bray-Curtis distance metric after data transformation (Tong et al., 2017). Two phylogenetic trees were constructed, with one for dominant/sub-dominant Symbiodiniaceae subclades (>1%) and the other for background subclades (0.1 to 1%), based on the Kimura 2-parameter model with uniform rates among sites using Maximum Likelihood in MEGA 6 and Bayesian inference in MrBayes (Ronquist et al., 2012;Tong et al., 2017).
Shannon diversity index of Symbiodiniaceae indicated that A. corymbosa, the five species of Faviidae, M. ampliata, H. exesa, and C. exesa had relatively high diversity (0.85-1.21), while P. verrucosa (0.44), P. lutea (0.20), M. efflorescens (0.14), and F. fungites (0.27) had low diversity (Wilcoxon rank-sum test, p = 0.032). This indicates that Symbiodiniaceae assemblages of the former species were higher diversity than the latter group. Although Cladocopium was the most represented among the background subclades (with the exception of P. verrucosa), each coral species contained multiple subclades belonging to different genera. For example, background types of A. corymbosa contained 99 subclades, 94 of which belonged to Cladocopium, and five of which belonged to Durusdinium. In P. lutea and M. efflorescens, fewer background subclades were detected (49 and 65, respectively), and more Symbiodiniaceae genera (Symbiodinium, Cladocopium, Durusdinium, Fugacium, Gerakladium, and clade I) were found in P. lutea. Although most relative abundance of these background subclades was <0.1%, the number and diversity of background subclades among corals showed that they contained different Symbiodiniaceae abundances and diversity (Figures S3, S4; Table S6).
Results of nMDS revealed a clear clustering pattern that varied significantly among coral species (Figure 3). The compositions of P. verrucosa and F. fungites were extremely different from other corals. P. lutea, M. efflorescens, and P. varians were similar, but were clearly different from E. lamellosa, H. exesa, and C. exesa. Symbiodiniaceae subclade compositions were similar among A. corymbosa, M. ampliata, and the five species of Faviidae, but clearly different from other coral species.

Variation in Zooxanthellae Density in the 15 Coral Species and Their Drivers
In our study, although there were significant differences in ZDs of the 15 coral species, the ZDs of all species were at low levels (0.8-1.2 × 10 6 cells cm −2 ) compared with those in the northern SCS (Li et al., 2008;Xu et al., 2017). This could be shaped by a variety of environmental factors (e.g., SST, nutrients, water transparency, salinity, or pH). Based on the in situ measurement of environmental data, we found that Xinyi Reef is a tropical CRR with high SST and transparency, low turbidity, and oligotrophic conditions. Generally, the main reason for low ZD at tropical reefs is the abnormally high SST that occurs in frequent El Niño under global warming (Hoegh-Guldberg and Salvat, 1995;Hoegh-Guldberg et al., 2007;Li et al., 2011). The third global coral bleaching event occurred from 2015/2016, resulting in severe damage to corals in tropical regions (Hughes et al., 2017). In May 2015, the seawater temperature was 30.4 ± 0.6 • C during the sampling period. Corals may be under threat of thermal stress with significantly reduced in zooxanthellae (Fujise et al., 2013;Decarlo et al., 2017). It exhibited lower ZD levels than other reef regions that are below 30 • C (Li et al., 2011). In summer, in the Luhuitou fringing reef of northern SCS, for example, ZDs of Porites, Acropora, and Pavona were ∼5 × 10 6 , 2 × 10 6 , and 3 × 10 6 cells cm −2 , which were ∼4 times, ∼2 times, and ∼3 times higher than our results, respectively (Xu et al., 2017). As for Acropora corals, which have low original levels of ZDs, the densities are lower than other previous reports from low-latitude tropical reefs of the Indo-Pacific, such as French Polynesia (Ladrière et al., 2014) and Kimberley, Australia (Verena et al., 2015). Even for the heat-tolerant Porites, Yu and Zhao (2006) found that the corals experienced several severe bleaching disturbances from evidence in the SCS over the past two centuries, suggesting that the main threat to these corals is high SST. Besides, in situ ecological observed in the southern SCS showed that 31-90% coral-symbiotic ZDs were lost over all these coral species during abnormally high thermal stress (Li et al., 2011;Yu, 2012).
Nutrients are also important factors influencing the coral-ZDs (Fabricius, 2005;Wiedenmann et al., 2013;Sawall et al., 2014;Ke et al., 2018). In our study, the DIN in this coral reef was 1.38 ± 0.15 µmol L −1 and the SRP was 0.06 ± 0.02 µmol L −1 during investigation. This indicates that nutrients are in low levels and oligotrophic conditions may limit Symbiodiniaceae reproduction and reduce their population (Ke et al., 2018). Other factors such as solar radiation and water transparency may also affect the ZDs as they directly affect the amount of sunlight available for photosynthesis of Symbiodiniaceae. In the southern SCS, solar radiation can be efficiently transmitted to corals with a high transparency (>20 m) and low turbidity (∼0.2 NTU). High light intensity possibly results in the excretion of coral-ZD (Brown, 1997;Hoegh-Guldberg, 1999), resulting in generally low ZD levels.

Variation in Dominant Symbiodiniaceae Types in Explored Coral Species and Potential Relationships With Coral Adaptability to Thermal Stress
The results from ZDs suggested that there were generally low coral-ZD levels in all coral species in the southern SCS. Although with lower ZDs in the branching P. verrucosa, we found that it was the main dominant species and survived well in this CRR. This suggests that ZD may not be the most important factor determining coral thermal adaptability in the southern SCS. In contrast, in our study, there were significant differences in dominant Symbiodiniaceae genera/subclades among these coral species based on the results of the Symbiodiniaceae ITS2 sequence alignments and OTU analysis. These variations of dominant Symbiodiniaceae types may effectively affect corals adaptability to thermal stress.
Recently, the characteristics of heat-tolerance and sensitivity in numerous Symbiodiniaceae at genus/subclade levels have been previously explored (Kemp et al., 2014;Silverstein et al., 2015;Swain et al., 2017). This contributed to our rationale for investigating coral adaptability to thermal stress among species. In the Indo-Pacific, many symbiotic subclades are considered to be heat-sensitive (e.g., C3, C7, and A13), while some subclades in Cladocopium (e.g., C15, C15-like types) and Durusdinium (e.g., D1, D1-4 and D1a) are considered to be heat-tolerant (e.g., Hume et al., 2015;Silverstein et al., 2015;Swain et al., 2017). Long-term tropical environmental exposure has forced corals to become associated with dominant heat-tolerant Symbiodiniaceae, giving them higher resistance to thermal stress (Kennedy et al., 2015;Hume et al., 2016;Brener-Raffalli et al., 2018). Generally, corals associated with Durusdinium can enhance their tolerance to thermal stress (Berkelmans and Oppen, 2006;Kennedy et al., 2015). For instance, Tong et al. (2017) found that the symbiotic dominant Symbiodiniaceae of Galaxea fascicularis belong to Durusdinium, which is significantly more resistant to thermal stress than the Cladocopium members associated with Montipora corals. In our study, P. verrucosa was associated with dominant Durusdinium of D1 and D1a, and P. lutea and M. efflorescens were associated with dominant C15. These Symbiodiniaceae types are considered to be highly adaptable to thermal stress (Hume et al., 2015;Silverstein et al., 2015;Swain et al., 2017). Based on coral evolutionary relationships (Figure S8), corals with closer phylogenetic relationships seem to associate with similar dominant Symbiodiniaceae types. This suggests that the choice of dominant subclade is mainly controlled by the coral host (Sampayo et al., 2008;Putnam et al., 2012;Klepac et al., 2015). Therefore, theoretically, corals associated with heattolerant Symbiodiniaceae may have high thermal tolerance.
The cause of coral species (e.g., P. verrucosa) becoming dominant can be understood by the variations in dominant Symbiodiniaceae types in the southern SCS. Our study suggests that the main reason of the branching P. verrucosa corals becoming dominant and surviving under thermal stress conditions is that they are associated with heat-tolerant Durusdinium. Similarly, the P. lutea and M. efflorescens associated with C15 may also have relatively high thermal tolerance and become dominant in the southern SCS.

Diversity of Background Symbiodiniaceae Types and Potential Relationship With Coral Adaptability to Future Global Warming
Symbiodiniaceae background types were significantly different among corals regardless of subclade types or diversity. The subclades in corals such as P. verrucosa and A. corymbosa were relatively highly diverse, but this was much lower in P. lutea, M. efflorescens, and F. fungites. In tropical reefs, corals may improve their adaptability to climate change by increasing the diversity of symbiotic Symbiodiniaceae. General corals such as P. verrucosa could increase their diversity by developing symbiotic relationships with different free-living Symbiodiniaceae (Baker, 2003;Putnam et al., 2012;Fabina et al., 2013). Horizontal transmission patterns are beneficial for these general corals to develop associations with various Symbiodiniaceae, including heat-tolerant types (Boulotte et al., 2016;Quigley et al., 2017). A rapid breeding strategy allows corals to obtain more heattolerant Symbiodiniaceae from the surrounding water and to increase survival in the short term Smith et al., 2017a). In our study, the dominant Symbiodiniaceae in P. verrucosa are heat-tolerant Durusdinium (e.g., D1 and D1a), and its background Symbiodiniaceae assemblage also include numerous heat-tolerant types, indicating that this species adapts by associating with a high proportion of heat-tolerant Symbiodiniaceae. Corals with high diversity can adapt to thermal conditions by altering the abundance of heat-tolerant background Symbiodiniaceae. A. corymbosa, F. palauensis, F. abdita, G. aspera, D. heliopora, and P. daedalea also belong to general corals and have numerous associated background types. Although their dominant subclades are Cladocopium C3u and Cspc, a variety of heat-tolerant background types (e.g., D1, C40, C15, and C15.6) were also detected, indicating high symbiotic diversity. These coral species include numerous Symbiodiniaceae including Symbiodinium, Breviolum, Cladocopium, Durusdinium, Fugacium, and Gerakladium. High diversity of Symbiodiniaceae may help these corals survive under thermal stress, despite not being dominant in the southern SCS.
In addition, previous studies have found that P. lutea and C15 are closely associated in the northern SCS and have observed a coevolution between the two (Ng and Ang, 2016;Gong et al., 2018). Consistently, we found that C15 was also the dominant Symbiodiniaceae subclade of P. lutea in the southern SCS. However, we additionally detected 49 background subclades, including a variety from Symbiodinium, Breviolum, Cladocopium, Durusdinium, Fugacium, and Gerakladium, implying that even coral species with high symbiont specificity are associated with diverse and adaptable background assemblages. These results indicate that P. lutea in the southern SCS can possibly adapt to high temperature environments and/or recover from bleaching events via relatively high symbiosis diversity under future global warming. Overall, our results provide an important basis for better understanding the coral-Symbiodiniaceae community population, composition, and diversity among coral species in the southern SCS and benefit for insight into variation in thermal adaptability among corals in field.

DATA AVAILABILITY STATEMENT
The data used in this paper can be obtained from Supplementary Materials or requested by emailing KY at kefuyu@scsio.ac.cn.

AUTHOR CONTRIBUTIONS
ZQ and KY conceived and designed the experiments and wrote the manuscript. ZQ and BC performed the experiments and analyzed the experimental data. ZQ, YW, JL, WL, LX, and XH contributed to reagents and materials. All authors edited and approved the manuscript.