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[Analysis of monosaccharide compositions in Heterosmilax japonica polysaccharide by precolumn derivation HPLC].

OBJECTIVE
To establish precolumn derivation HPLC method to determine the monosaccharides in Heterosmilax japonica polysaccharide.


METHODS
Heterosmilax japonica sample was hydrolyzed with trifluoroacetic (TFA)and derivated by 1-phenyl-3-methyl-5-pyrazolone (PMP). The monosaccharide composition of Heterosmilax japonica polysaccharide was carried out by reversed-phase technique on a Kromasil C18 column with a mobile phase composed of 100 mmol/L ammonium acetate buffer (pH5.5) and acetonitrile in the ratio of 77: 23. The detection was carried out at 245 nm.


RESULTS
The Heterosmilax japonica polysaccharide composed of mannose, rhamnose, glucose, galactose and xylose with amolar ratio of 66: 1:7590: 452: 528.


CONCLUSION
The HPLC method with precolumn derivatization is appropriate for the analysis of monosaccharide composition of Heterosmilax japonica polysaccharide and the method is simple, quick and accurate.