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Automated determination of serum oxytocinase activity.

An automated kinetic method is described for determination of oxytocinase activities in the sera of pregnant women. The reaction mixture consists of serum in phosphate buffer and the substrate L-cystine-bis- p -nitroanilide. The absorbance increase, due to the appearance of the reaction product, p -nitroaniline, is recorded during 2 min. With this method, 25 determinations per hour can easily be done.