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Organotypic Co‐Cultures of Rat Locus Coeruleus and Hippocampus

Slices from the brainstem at the level of the locus coeruleus and from the hippocampus of 5–7 day old rats were co‐cultured using the roller tube technique. After 2–6 weeks in vitro the co‐cultures were examined with antibodies raised against tyrosine‐hydroxylase (TH). The cultures derived from the brainstem consistently contained a bilateral cluster of TH‐positive neurons with 3–5 long slender dendrites. These neurons typically gave rise to several fine varicose fibres reminiscent of catecholaminergic axons. A morphologically distinct group of TH‐positive neurons was detected in the hippocampal slices. The vast majority of them were located in the subicular region and a smaller number in the CA1/CA3 region of the hippocampal explant. TH‐positive neurons were also present in mono‐cultures of hippocampus or brainstem.