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SEPARATION, IDENTIFICATION AND CHARACTERISATION OF EXTRACTIVES FROM SUGARCANE (SACHARRUM OFFICINARUM) LEAF

The separation, identification and characterisation of the extractives from green and brown leaves of sugarcane plant (Sacharrum officinarum) are described. Green leaves were extracted by boiling in water, sonicating in a 1:1(v/v) methanol:water solution and 1:1(v/v) acetone:water solution and by the soxhlet technique. Dried leaves were extracted by sonication in a 1:1(v/v) methanol:water solution. Flavonoids and phenolic compounds in the green and dried leaf extracts were analysed, identified and characterised by use of Ultraviolet spectrophotometry and High Pressure Liquid Chromatography with photodiode array detection. The efficiency of the extraction methods were compared to previously reported values. The soxhlet technique gave the highest extraction of flavonoids and phenolic compounds.