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Analysis of the hot spots of coated pits in live cells

Coated pits carry important information about plasma membrane and endosomes. They remove proteins and lipids from the plasma membrane and transport them to the endosomal compartment. It is of interest to get a thorough understanding of these pits. In this paper, we consider two kinds of proteins in coated pits: clathrin and AP-2. We propose a detection method to extract as many coated pits trajectories as possible. It is observed that we can predict whether or not a pit will appear at certain "hot spot" at a certain time.