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DNA isolated from plastic embedded tissue is suitable for PCR.

Plastic embedding of routinely formalin fixed tissues provides an excellent morphology and is used in many centers especially for routine histology of bone marrow biopsies (1). Since recent reports have shown the feasibility of DNA isolated from fixed paraffin embedded tissue for application of PCR, we have extracted DNA from bone marrow trephine biopsies fixed in 10% formalin, decalcified in EDTA, embedded in Technovit 700 (hydroxyethylmethacrylate; HEMA; Kulzer, FRG) and stored between 2 months up to 4 years at room temperature to test also for the influence of different degrees of polymerization of the embedding medium.