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Physiologic saline solution, Normosol R pH 7.4, and Plasmanate as reconstituents of packed human erythrocytes.

The authors compared the effects of three reconstituent solutions (Normosol R pH 7.4, physiologic saline solution, and Plasmanate) on hemolysis and cellular morphology of young (8-10 days' storage) and old (22-26 days' storage) packed human erythrocytes (PE). These variables were tested at 22 and 37 C and at two dilutions )1 part PE:1 part reconstituent and 2 parts PE:1 part reconstituent). Plasmanate produced unacceptably high levels of hemolysis, particularly of older PE, and should not be used as a reconstituent. Normosol R pH 7.4 and physiologic saline solution were compatible with both young and old PE, producing similar changes in hemolysis and erythrocytic morphology, Therefore, Normosol R pH 7.4 may be used as a diluent for PE when rapid and massive infusions are needed.