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Effect of 2-mercaptoethanol on the mixed leukocyte reaction in man. Probable effect on blastogenic factor stabilization.

When 2-mercaptoethanol (2-ME) is added to human mixed leukocyte cultures at the beginning of the culture period, only a very weak effect is seen. When 2-ME is added after a few days' culture, a marked enhancement of cellular transformation occurs. 2-ME can also enhance the effect of blastogenic factor (BF) containing media and it is possible that this effect depends on a stabilization or protection of BF preventing the break-down of BF in cellular systems.