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Morphological Identification of Langerhans Cells in Mouse Epidermal Cell Suspension by Scanning Electron Microscopy

The distribution of Iak antigens on suspended epidermal cells prepared from C3H/He mice by trypsinizing their ear skin was examined by the scanning immunoelectron microscopic method using antibody against the antigen and antibody bacteriophage T4 conjugates as a visual marker. Iak antigens were found to be distributed diffusely over a cell type with a relatively flat shape and a number of microvilli and ruffles. These cells are considered to be Langerhans cells. Significance of these findings is discussed.