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OPTIMIZATION OF LIPID PRODUCTION BY OLEAGINOUS YEAST USING RESPONSE SURFACE METHODOLOGY

The effects of growth parameters of Rhodotorula graminis TISTR 5124 in batch fermentation were studied and optimized for lipid production by using response surface methodology via a BoxBehnken Design. Values of the fermentation parameters affecting the lipid production were varied as follows: carbon sources (glucose, glucose and glycerol and glycerol), temperatures (28, 30, and 32 o C) and shaking speeds (150, 200, 250 rpm). Fermentation was carried out in 100 mL Erlenmeyer flasks with a 24 h cultivation time. After eliminating insignificant terms, we found that a good fit (R 2 = 0.7555) for lipid production was given by the quadratic regression relationship:Lipid = (4.00* Temperature)- (4.75*Carbon) + (0.70*Shaking speed) – (0.02*Temperature*Shaking speed) -116.0. The results showed that lipid production was significantly influenced by carbon source (p < 0.0001). For the range of conditions studied, we found that the highest yield of lipid was 17.40 g/L, which was obtained using glycerol as sole carbon source at 10 g/L, a temperature of 28 o C and a shaking speed of 239 rpm. We found that a good fit (R 2