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New Approach to Improve X-Ray Diffraction Pattern of Protein Crystal Using UV-Laser Ablative Processing

A newly proposed processing technique for protein crystals, Pulsed UV Laser Soft Ablation (PULSA), is found to be practically useful for X-ray diffraction analysis. We experimentally confirmed that a diffraction pattern from a cracked protein crystal was considerably improved by eliminating the damaged section with the PULSA processing. This technique will enable us to easily process protein crystals mounted on X-ray diffraction equipments without any extra mechanical handling of the crystals.