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Effects of Light, Surface Sterilization, and Fungicides on the Germination of Black Huckleberry Seeds

Abstract Germination tests were conducted on seeds of black huckleberry (Vaccinium membranaceumDouglas ex Hooker) to determine the effects of light exposure, surface sterilization, and fungicides. In total darkness, approximately 2% of seeds germinated. A single 24-h exposure to fluorescent light increased the rate to 37.4%. Daily exposure to 12 h of light for 77 d produced average germination rates of 78.6%. Surface sterilization in diluted household bleach (0.5% sodium hypochlorite) eliminated contamination by microorganisms without reducing germination percentages, but altered the germination versus time curves by shortening the lag period between sowing and radicle emergence. Treating seeds with captan or mancozeb fungicides increased the time necessary for germination and reduced the percentages of seeds that germinated. Light exposure is required for maximum seed germination. For germination trials where only maximum cumulative germination percentages are of interest, surface sterilization with bleach is a valuable tool. Seed treatment with captan or mancozeb fungicides can inhibit germination.