{'fraction_non_alphanumeric': 0.020480854853072127, 'fraction_numerical': 0.005342831700801425, 'mean_word_length': 5.175824175824176, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Fabrication of Paper-Based Microfluidics by Single-Step Wax Printing for Portable Multianalyte Bioassays

In this paper, we initially report a new type of wax printing method for rapid fabrication of microfluidic devices in paper using a commercially available, cheap, minitype (home-use) CO2 laser engraving machine. This method combines the two core operations commonly involved in all previous wax printing methods, namely the printing and heating (melting) of wax patterns into one operation of engraving home-made wax slice (put in contact with the surface of paper) by laser. The heat produced by the laser makes the wax being engraved melt and then spread into paper to form complete hydrophobic barriers which are used to define the hydrophilic flow channels or separate test microzones. Under the optimized experimental conditions, a typical device on a 3 cm × 3 cm piece of paper could be fabricated separately within ~320 sec and is ready for use once the engraving process is completed. The fabrication resolution and multiplexed analytical capability of the wax-patterned paper were additionally characterized.