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Electrochemical Detection of Circulating Tumor Cells Based on DNA Generated Electrochemical Current and Rolling Circle Amplification.

Circulating tumor cells (CTCs) are an important indicator for tumor diagnosis and tumor metastasis. However, the extremely low levels of CTC in peripheral blood challenges the precise detection of CTCs. Herein, we report DNA generated electrochemical current combing with rolling circle amplification (RCA) as well as magnetic nanospheres for highly efficient magnetic capture and ultrasensitive detection of CTCs. The antiepithelial-cell-adhesion-molecule (EpCAM) antibody modified magnetic nanospheres were used for enrich and capture CTCs. The following binding of aptamer onto the CTC surface and the subsequent RCA assembled significant amount of DNA molecules onto electrode. The reaction of the DNA molecules with molybdate can then form redox molybdophosphate and produce electrochemical current. Using breast cancer cell MCF-7 as model, the sensor displays good performances towards detection of MCF-7 that spiked into peripheral blood. The signal amplification strategy integrated with magnetic nanosphere platform exhibit good performance in efficient capture and detection of CTCs, which may find wide potential in cancer diagnostics and therapeutics.