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THE EFFECT OF OXIDANT INJURY ON THE LYMPHOBLASTIC TRANSFORMATION OF HUMAN LYMPHOCYTES *

Abstract— We have studied the effect of oxidant injury on the lymphoblastic transformation and glucose metabolism of human lymphocytes. Lymphocyte cultures were incubated with xanthine oxidase and xanthine, an enzyme system known to generate several highly reactive oxygen compounds. Lymphoblastic transformation to phytohemagglutinin was quantitated by the stimulation of DNA synthesis as indicated by the 3H‐thymidine uptake of the cultures at 3 days.