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ASPECTS OF ACETYLCHOLINE METABOLISM IN THE ELECTRIC ORGAN OF TORPEDO MARMORATA

Abstract— —The synthesis of acetylcholine and its compartmentation were studied in the electric organ of Torpedo marmorata. When electric organ was homogenized in iso‐osmotic NaCl‐sucrose some 55 per cent of its acetylcholine content was lost unless very potent cholinesterase inhibitors were present. Slices of electric organ incubated in a suitable medium were found to synthesize radioactive‐labelled acetylcholine from [N‐Me‐3H] choline. The specific activity of the labelled acetylcholine was higher in the trichloracetic acid extract of the organ slices than in an NaCl‐sucrose homogenate. Acetylcholine‐containing vesicles isolated from the NaCl‐sucrose homogenate contained labelled acetylcholine with about the same specific activity as the parent homogenate. There was thus a fraction of acetylcholine in the incubated tissue of higher specific radioactivity that was lost when the tissue was homogenized. The acetylcholine‐containing vesicles lose their acetylcholine when submitted to gel filtration under hypo‐osmotic conditions. On standing at 5°C there were only small losses of acetylcholine from the vesicles but at 20°C the losses were substantial. Vesicles containing labelled acetylcholine were studied. On gel filtration under iso‐osmotic conditions there was a considerable loss of labelled acetylcholine without a concomitant loss of bio‐assayable acetylcholine. The pools of radioactive and bio‐assayable acetylcholine are therefore not homogeneous in the vesicles as isolated.