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A vectorial structured light holographic optical trap for control of fluorescent particles

Here we create a holographic trap that allows trapping with scalar Gaussian and vortex beams, as well as vectorial combinations of the two. When equally weighted, the resultant is a propagation invariant flat-top beam trap. The set-up includes a spatial light modulator in an interferometer to combine the beams, an inverted microscope for imaging, and a photon counting fluorescence detection stage. We outline the functionality of this system, measure the trap stiffness for some example beams, and show in-situ fluorescence measurement from quantum dots attached to micro-scale beads.