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Isolation and Identification of Pure Bioactive Compounds from Cuscuta reflexa Grown on
Nerium oleander Host Plant by Flash Column Chromatography and GC-MS/MS Analysis

In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was
used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used
as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and
two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were
identified in Cuscuta reflexa using NIST library on the basis of area percentage.