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Biochemical Characterization of Starch Excess4 from Storage Crops

Starch is a water‐insoluble branched polymer of glucose. Its degradation requires the concerted action of glucan phosphatases and glucan dikinases. Reversible starch phosphorylation allows for the solubilization of the starch surface, allowing for amylase to degrade starch into maltose units. However, our understanding is limited on how these enzymes contribute to starch degradation in storage crops such as potato and cassava. In this study, we biochemically characterized cassava and potato SEX4 enzymes using enzymatic assays and differential scanning fluorometry. We determined the kinetics of SEX4 using both generic and physiologically relevant substrates. We also explored the utilization of these glucan phosphatase orthologs to enhance starch degradation in‐vitro. Our findings reveal that cassava and potato SEX4 show similar allosteric activation mechanisms but varying abilities to degrade starch under in‐vitro conditions.