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Determination of sulfonamides and local anesthetics with 9=chloroacridine by quenching fluorometry.

Sulfonamides and local anesthetics containing a primary aromatic amino group react with 9-chloroacridine to yield aminoacridine hydrochlorides. The formation of these derivatives results in quenching of fluorescence of the 9-chloroacridine reagent solution. Monitoring the fluorescence at activation and emission wavelengths of 385 and 420 nm., respectively, enables one to analyze drug in the 10−5−10−6M range. The method was applied to various sulfonamides, local anesthetics, and mixtures of pharmaceuticals containing these entities. A comparative study of this technique was made with other known fluorometric procedures.