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Triple Helix of β-D-Glucan from Lentinus Edodes in 0.5 M NaCl Aqueous Solution Characterized by Light Scattering

Abstractβ-(1→3)-D-glucan with (1→6) branching (L-FV-I) from Lentinus edodes in water was degraded into seven fractions of different molecular weights by ultrasonic irradiation. Weight-average molecular weight Mw, radius of gyration ‹s2›z1/2 and intrinsic viscosity [η] of the β-D-glucan and its fractions in 0.5 M NaCl aqueous solution and dimethylsulfoxide (DMSO) were studied by multi-angle laser light scattering (MALLS), GPC combined with MALLS, and viscometry. Mw dependence of [η] for the glucan in 0.5 M NaCl aqueous solution was represented approximately by [η]=7.69×10−6Mw1.32 (cm3 g−1) at Mw from 1.87×105 to 1.20×106 at 25°C. GPC chromatograms of the glucans in aqueous solution contained two peaks, a main peak corresponding to triple-stranded chains with molecular weight Mw,m, and small second peak corresponding to fragments of single chains with Mw,s (about 20±5% content). Analysis of Mw,m and ‹s2›z,m1/2 in term of the known theory for wormlike chains yielded 2180±100 nm−1, 120±10 nm and 0.31 nm for molar mass per unit contour length ML, persistence length q, and contour length h per main-chain glucose residue, respectively, which agree closely with theory data of triple-helical chains and reported parameters for triple-helix schizophyllan in 0.01 M NaOH aqueous solution. The ratios of Mw,m in 0.5 M NaCl to Mw in DMSO were calculated to be roughly 3. The predominant species of the glucan in 0.5 M NaCl aqueous solution exist as triple-helical chains with high rigidity, and in DMSO as single-flexible chains.