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ATP induced temperature‐dependent inhibition on contractile responses to electrical field stimulation in rat vas deferens

The contractile responses of prostatic and epididymal portions of rat vas deferens evoked by electrical field stimulation(EFS) were studied at four temperature condition of 27, 32, 37 and 42°C. Different contractile responses were found in these two portions and temperature distinctly affects the EFS‐stimulated contraction. Lowering the temperature caused increase in basal tension of the smooth muscle and attenuated the phasic contraction to EFS in both portions, though the total amplitude of contraction were not much affected. ATP (1–1000μM) inhibited the EFS‐evoked contraction in both prostatic and epididymal portions of rat vas deferens in a dose‐dependent manner; this inhibitory effect was significantly more prominent in prostatic portions than epididymal protion. In prostatic portion, the maximal inhibition of ATP(100μm) were reached at temperature of 27°C, while the inhibitory effect of ATP showed little difference in epididymal portion at four temperature conditions. A P2 receptor antagonist, Pyridoxalphosphate‐6‐azophenyl‐2′,4′‐disulfonic acid (PPADS, 10μm) partially reduced the inhibitory effect of ATP(100μm) at both portions in all four temperature conditions. Together, in our experiments, ATP caused temperature dependent‐inhibition of contractile response to EFS in rat vas deferens, which was distinct in prostatic and epididymal part of vas deferens. The P2 receptor mediated relaxation may involve in this kind of inhibition.