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Properties of Q -replicase protein synthesized in a cell-free system.

The largest polypeptide obtained as a product of Qβ RNA-stimulated cell-free protein synthesis is the replicase (R) protein. The R polypeptide synthesized in vitro has a chain length of approximately 550 residues and is identical in size to the β chain of purified replicase. From its electrophoretic behavior at pH 8.5, a net charge of -27 and a cysteine content of > 15 residues can be deduced. A portion of R polypeptide synthesized in vitro binds to added Qβ RNA and this binding is more resistant to increased ionic strength than the binding of the positively charged coat protein to Qβ RNA. Another portion of the R polypeptide binds to some constituent of the Escherichia coli extract to form a 20-S complex.