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HPTLC Monitoring of the Biocatalytic Synthesis of Quinoxalinone Derivatives in a One-Pot Reaction

High-performance thin-layer chromatography has been used to follow the synthesis of some quinoxalinone derivatives by biocatalysis in a one-pot reaction, as an alternative route to the poor Hinsberg reaction. The progress of the one-pot reactions can be followed by HPTLC without previous isolation of the different intermediates arising from them. Biocatalytic synthesis of quinoxalinone derivatives leads to an irreversible reaction which gives a single product of high purity and yields in excess of 90–95%.