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Effect of the Base Layer in the Cylinder-Plate Method for Analysis of Penicillin.

Using the Sarcina lutea protocol for detection of penicillin, experiments comparing zones of inhibition in the presence and absence of the base layer indicated that omission of the uninoculated base layer enhanced detection of smaller concentrations of the inhibitory agent. It is, therefore, suggested that the requirement for the base layer be removed from the official, approved procedure.