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Fluorescence spectroscopy and imaging of pH in vivo using a dual emission fluorophore (C-SNAFL-1)

Fluorescent intracellular pH indicators have been widely applied in recent years to monitor changes in intracellular pH. More recently, we have proposed the use of dual-excitation pH-fluorescent indicators (5,6-CF: 5,6- carboxyfluorescein or BCECF: 2',7'-bis-carboxyethyl-5,6-carboxy-fluorescein) to monitor pH on animals. This study aimed to evaluate a dual-emission fluorophore (C-SNAFL-1: 5' (and 6') carboxy-seminaphthofluorescein) for pH monitoring in-vivo. In that case, one excitation wavelength and two emissions are chosen to give a pH-dependent emission intensity ratio.