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Extended Depth of Field Preserving Color Fidelity For Automated Digital Cytology

This paper presents a multi-channel Extended Depth of Field (EDF) method for digital cytology based on the stationary wavelet transform. With a coefficient selection rule adapted to a precise color recovery, a sharp image can be reconstructed even on images with transparent overlapping cells. The precision and the color fidelity of the proposed method is analyzed. Moreover, an experiment demonstrating the necessity of volume analysis in cytology to achieve precise segmentation on cell clumps is conducted, and the importance of color fidelity in this context is asserted. The proposed method was tested on pap-stained urothelial cells and gray-scale cervical cells with important overlapping.