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Effects of Tactile–Kinesthetic Stimulation on Low Birth Weight Neonates

Objective Low Birth Weight [LBW] (1500gr ≤ Birth Weight ≤ 2499 gr) is one of the most serious health problems in neonates. These neonates need complementary interventions (e.g. tactile-kinesthetic stimulation) to promote development. This study was conducted to determine the effect of Tactile-Kinesthetic Stimulation (TKS) on physical and behavioral development of Low Birth Weight neonates. Methods This was a randomized controlled trial with equal randomization (1:1 for two groups) and parallel group design. Forty LBW neonates were randomly allocated into test (n = 20) and control (n = 20) groups. TKS was provided for three 15 minute periods per day for 10 consecutive days to the test group, with the massages consisting of moderate pressure strokes in supine and prone position and kinesthetic exercises consisting of flexion and extension of limbs. All measurements were taken before and after completion of the study with the same equipment (Philips electronic weighing scale with an accuracy of ±5 grams and Brazelton Neonatal Behavioral Assessment) and by the same person. Findings There was a trend towards increased daily weight gain, but without statistical significance. On the Brazelton scale, the test group showed statistically significant improved scores on the ‘motor’ (P-value <0.001) and ‘regulation of state’ (P-value = 0.039) clusters after the 10 days TKS. Conclusion TKS has no adverse effects on physiologic parameters and gives better adaptive behavior of LBW neonates compared to those without TKS.


Introduction
With developments in medical technology especially in neonatal intensive care, the survival rate of premature and low birth weight infants has been markedly improved. Nevertheless, such infants remain at an increased risk for later poor developmental outcomes as the birth weight decreases and they are at high risk for major developmental disorders such as cerebral palsy and minor motor disorders such as developmental coordination disorder and minor neurological dysfunction [1,2] .
Many studies reported that intervention may be most effective if it is applied during infancy when there is high plasticity of the brain [3] . A number of studies have demonstrated that early intervention can facilitate early mother-infant relationship and results in beneficial developmental outcomes in non-brain-injured low birth weight neonates [4,5] . Differences in the time of the receiving sensory inputs seem to change the connectivity and the structure of the cerebral cortex and the responsiveness of neonates to tactile stimulation Original Article in the first days of life is greater than any other sensory modality because skin is the largest sensory organ and the tactile system is the earliest sensory system to become functional [6,7] . Furthermore, growth and cerebral maturity depend on neurotransmitters that are responsive to stroke and gentle tactile stimulation [7] .
The typical massage used in neonates is a gentle stroking with moderate pressure of parts of the body combined with kinesthetic stimulation that consists of passive motion of the limbs [8] . Early stimulation given to neonates will change the growth of the brain cells, improve adaptive behavior, and finally cause the achievement of the optimal development of their age [6] . Neonates continually communicate their level of stress and stability in relation to what is happening to and around them therefore, caregivers must be sensitive to stress behaviors versus stable behaviors. Behavioral development is very important and is prerequisite for neurodevelopment [9] . Adaptive behaviors are infant's ability to involve with environmental stimulus and show cortical function that examined with Neonatal Behavioral Assessment Scale (NBAS) or Brazelton scale. Brazelton scale is sensitive to changes and is a good scale for evaluation of neonatal behaviors [10] .
Despite the several benefits pointed out by studies in the area, lack of consensus on the mechanisms responsible for the clinical and behavioral improvements or the specific intervention scheme which would be more effective and suitable for each situation according to gestational age or birth weight were the reasons why massage was not yet recommended as routine procedure stimulation for neonates. This study was designed to find out the effects of ten-day tactile stimulation on physical and behavioral changes in low birth weight neonates.

Subjects and Methods
This was a Randomized Controlled Trial (RCT) study type. The study was carried out on LBW neonates in Akbar-Abadi hospital, Tehran. The study sample was consisted of 40 inborn LBW neonates, who had to meet the following selection criteria for inclusion: 1) Birth weight (BW) >1500 g and <2499g, 2) Age of neonates 1 day, 3) Absence of congenital anomalies and neuromuscular disorders, 4) Medically stable with no requirement of drugs (other than mineral and vitamin supplements) or any specific interventions. We excluded neonates whose parents tended to draw their neonates of the study for any reason and at any time.
Change in habituation was selected to calculate sample size with an α-value of 5% and power of 80%. The analysis accounted for a 20% dropout rate. Twenty neonates per group were needed to detect clinically worthwhile effects. It seemed difficult to choose the participants with such inclusion criteria but many eligible neonates were actually hospitalized for a long time because of bad parenting and/or economic and cultural issues. After informed consent was taken from the parents, neonates were randomly assigned to the treatment and control group (n=20) based on a stratification of gestational age, birth weight, birth length, head circumference at birth, gender, Apgar (1 and 5 min), premature or IUGR. The treatment group received TKS for three 15 minute periods per day for 10 consecutive days.
The study was approved by the Research Ethics Committee of Rehabilitation College of Tehran University of Medical Sciences. After gathering of clinical data, all neonates were evaluated by NBAS and clinical data and results of evaluation were collected.
This study used the massage therapy protocol according to Field et al [11] . Massage therapy was begun on the first day of life and continued for 10 consecutive days. The massage sessions comprised three segments which were two fiveminute phases of tactile stimulation and one fiveminute phase of kinesthetic stimulation given during the middle phase. During tactile stimulation, the neonate was placed in prone position and stroked with the fingers of both hands of therapist for 5 one-minute periods over each region in the following sequence: 1) from the top of the head to the neck, 2) from the neck across the shoulders and back to the neck, 3) from the upper back to the buttocks and revere to the upper back, 4) from the thigh to the foot to the thigh on both legs simultaneously, and 5) from the shoulder to the hand to the shoulder on both arms simultaneously. For the kinesthetic phase, neonate Neonates' characteristics were compared using the Mann-Whitney U-test and t-test for continuous data and Fisher's exact test for discrete data. The independent t-test was used to analyze the difference between pre and post intervention in the cluster scores of the NBAS and weight (between groups) and the paired t-test was used to analyze the difference within group. The statistical software SPSS (version 18) was used for statistical analysis. All values were tabulated as averages (mean) with standard deviation (SD). For all analyses, the significance level was 0.05 and confidence interval 95%.

Findings
As shown in Table 1 and 2, neonates in both the treatment and the control group were matched  5 min), premature or IUGR and neonatal behavior. Also two groups were matched for socio-economic and cultural status. The mean birth weight and gestational age for neonates were 2015 (±309.95) grams and 33.65 (±1.93) weeks. According to the results (Table 3), treatment group was more mature in 'motor' and 'state regulation' and there was statistically significant difference between the 2 groups in these clusters. Weight at 10 th day (after study) was 1930.0 (±338.5) gr and 1945.5 (±299.8) in treatment and control group, respectively. There was no significant difference in weight gain in the two groups (P= 0.2).

Discussion
The present study assessed the clinical and behavioral aspects of low birth weight neonates during hospital stay. There are many studies about the effect of tactile stimulation on growth development of infants. The majority of studies have shown improvement in the clinical parameters specially weight gain because of increasing of ornithine decarboxylase, an important enzyme involved in protein synthesis, with tactile stimulation [11][12][13][14][15] . Also, a Cochrane meta-analysis has shown in average a greater weight gain of 5 g/day in treatment groups than in controls [15] . The results of the present study point to a tendency for an increase in weight gain for LBW neonates who received tactile-kinesthetic stimulation, but without statistical significance (Fig. 2). This is consistent with the reports of Lee (2006) [5] and Andreia et al (2010) [7] . One factor which might have contributed to this result is the weight loss due to loss of extracellular fluid during the first week of neonate's life [18] .
Some studies have reported that stimulation may adversely affect physiologic parameters in preterms and produce apnea [17] while others have not shown these [13,14] . In the present study, according to necessary monitoring for each neonate and also opinion of expert supervisor of NICU, the stimulation had no adverse effects on physiologic parameters like temperature, heart rates, apnea and respiratory rate.  In the behavioral aspects, there are some studies. A number of studies have reported better state regulation with tactile-kinesthetic stimulation for 10 days in neonates [4,6,13] and the result of this study shows treatment infants are more mature in state regulation compared to control group. In other words, the neonates who received TKS improved their abilities to be less irritable and fussy and showed less stress behaviors. These neonates had a higher capacity for maintaining stable state organization.
Also our results in motor is consistent with those of Wahyutami et al [6] and Field et al [11] . They reported that treatment infants showed better performance in motor behavior. The other behavioral results (orientation, habituation, range of state, autonomic stability and reflexes) in this study are inconsistent with Wahyutami et al [6] , Field et al [11] and Mathai et al [13] and only consistent with Ohgi et al [4] . It seems possible that reasons which have contributed to these results are participation of mothers in interventions and their interaction with their neonates and long term follow up.
Our study had some limitations. Some of selected neonates were discharged before 10 days and did not continue their intervention and were drawn from sample. It was also very difficult to match the two groups due to the inclusion criteria.

Conclusion
TKS has no adverse effects on physiologic parameters and gives better adaptive behavior of LBW neonates compared to those without TKS. These data provide evidence for the effectiveness of TKS in improving infant's behavior and a trend to enhance mature behavior.