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Serum proteins in human squamous epithelium

A new immunoperoxidase staining technique of high specificity (the labelled antigen method) has been used for the detection of serum proteins in the epithelium of human skin and mucous membranes. The majority of specimens contain cells showing strong diffuse intracellular staining. These cells usually lie in the upper mid‐zone of the epithelium and are often characterized by vacuolated or pyknotic nuclei. Double immunoenzymatic labelling (using alkaline phosphatase and peroxidase) showed that the different proteins are usually present in the same cells. A less frequent pattern of staining took the form of delicate strands of intercellular IgG.