{'fraction_non_alphanumeric': 0.030349794238683128, 'fraction_numerical': 0.020763187429854096, 'mean_word_length': 5.220186154741128, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Associations between self-reported sleep, wellbeing and physical activity in Irish adolescents

Growing evidence suggests sleep plays an important role in the development of healthy adolescents, with increased interest in the associations between sleep and mental health. Higher duration and quality of sleep has been suggested as a mechanism for increased wellbeing in adolescents. Cross sectional data was collected from 5,661 Irish adolescents. 55% of Irish adolescents reported meeting the guidelines for adolescents of 8-10 hours per night. This was found to decrease with age. Higher duration and quality of sleep was positively associated with wellbeing and negatively associated with symptoms of anxiety and depression. A higher frequency of physical activity was associated with longer duration and higher quality of sleep. 9-10 hours of sleep was associated with the highest levels of wellbeing and lowest symptoms of anxiety and depression. The relationship between physical activity and increased wellbeing may be impacted by physical activity leading to higher durations and quality of sleep.


Introduction
Growing evidence suggests that sleep plays a crucial role in the development of healthy adolescents (12-19 years), with a particular emphasis on the regulation of important daily cognitive functions (such as attention, vigilance, problem-solving, etc), mood and emotional wellbeing (Dahl & Lewin, 2002). Many epidemiological and laboratory studies also provide preliminary evidence for the potential role of sleep curtailment in adolescents and children with obesity (Litsfeldt, Ward, Hagell & Garmy, 2020). The role of sleep is more complicated in the adolescent population due to higher sleep needs coupled with the growth-and lifestylerelated sleep changes, such as social lives and shifts in circadian rhythms, seen at this developmental stage of life (National Sleep Foundation, 2019). The trend over time has been towards insufficient sleep in the younger population, which may be further increasing the burden of short sleep-mediated outcomes including both physical and mental ill health (Magee, Caputi & Iverson, 2014). The US National Sleep Foundation's recommended duration of sleep for adolescents is 8-10 hours per night (Hirshkowitz et al., 2015).
Research focusing on sleep loss or short-sleep duration has found connections to both cognitive and emotive issues such as impaired capacity to learn (Gomez, Newman-Smith, Breslin & Bootzin, 2011); decreased academic performance (Dewald, Meijer, Oort, Kerkhof & Bogels, 2010); and symptoms of depression (Gangswich et al., 2010). Other research that focuses on depression and depressive symptoms in children and adolescents have found that sleep disorders are common although disordered sleep can both stem from, and cause elevated symptoms of depression (Liu et al., 2007) with the majority of studies focusing on the specific relationship between short sleep duration and depression focusing on adolescents (Giannotti, Cortesi, Sebastiani & Ottaviano, 2002). One of the most influential investigations found that adolescents with parent set bedtimes of midnight or later were 24% more likely to suffer from depression and 20% more likely to have suicidal ideation than adolescents with parent set bedtimes of 10:00pm or earlier (Gangswich et al., 2010). The bidirectional relationship, where disordered sleeping impacts mental health outcomes and symptoms of depression and anxiety impact sleep has also been demonstrated in non-clinical child and adolescent populations (Muiris, van der Pennen, Sigmond & Mayer, 2008).
40% of Irish adolescents have reported elevated symptoms of anxiety and depression (Murphy, Sweeney & McGrane, 2020) although higher levels of physical activity were associated with lower symptoms of both. Lubans et al., (2016) proposed a number of mechanisms that seek explain the impact of physical activity on mental health and wellbeing, one of which is based on sleep, where increased quality and duration of sleep is positively associated with increased wellbeing. A recent meta-analysis of the effects of physical activity on sleep found that bouts of acute exercise had benefits on sleep duration and sleep quality while regular exercise, of any intensity, had beneficial effects on sleep duration and efficiency (Kredlow, Capozzoli, Hearon, Calkins & Otto, 2015). An investigation of physical activity and sleeping patterns also found that adolescents who engaged in 60 minutes or more of physical activity per day had increased odds of meeting sleep recommendations than those who did not (Foti, Eaton, Lowry & McKinight-Ely, 2011). Another cross-sectional examination of US adolescents found that 66% of boys and 75% of girls do not meet physical activity recommendations which was associated with lower odds of achieving adequate sleep (Kenney & Gortmaker, 2017). Similar findings were found in European adolescents as insufficient and poor sleep patterns are common throughout the European Union (Gariepy et al., 2020). Adolescents achieving the recommended duration of sleep range from 32% in some countries (Poland) to 86% in others (Flemish Belgium) with no specific data collected in Irish adolescents. No previous studies have explored the association between duration of sleep and symptoms of mental health or physical activity in Irish adolescents.
The aim of this study was to investigate sleep duration, by year and gender, among adolescents in Ireland. This research will also examine associations between sleep, physical activity, and mental health outcomes.

Method
Cross sectional data were drawn from the Physical Activity and Wellbeing Study (PAWS) (Murphy, Sweeney & McGrane, 2020). Data collection took place during September and October 2019. The study was approved by the DCU Ethics Committee (DCUREC/2019/107) prior to any data being collected. 144 schools initially registered interest in taking part. 65 schools were unable to participate fully due to either: commitments to other research; availability of time; or lack of Principal consent. The remaining 79 schools who registered interest represent 11% of the post-primary schools in Ireland. 5661 participants were recruited from these 79 schools throughout the Republic of Ireland with a minimum of one school from each of the 26 counties in Ireland giving a very good geographical spread. The number of student responses in each school ranged from 24 to 232.

Data Collection
Written consent from each school's Principal was sought prior to the distribution of questionnaires. Parental consent forms and plain language statements were given to all students prior to the questionnaire being issued. Students were administered a self-report questionnaire, which included well-established and validated instruments to assess sleep, physical activity, wellbeing and symptoms of depression and anxiety. Participants were informed that their responses would be treated in strictest confidence and that all responses were entirely anonymous. They were encouraged to take time, reflect on their answers, and to be as honest as possible. All questionnaires were administered in school through an online form and could be completed via desktop computer, laptop, tablet or mobile phone. An option to contact the National Education Psychologists Service (NEPS) was offered to participants after completion of the questionnaire. The means of administering the questionnaire was at the discretion of each participating school.

Mental health
Depressive symptoms: Severity of depressive symptoms was measured via the Beck Depression Inventory (BDI). Items of this instrument assess specific symptoms of depression experienced over the preceding two weeks. Each question was scored from 0 to 3, indicating the severity of the symptom, with total scores ranging from 0 to 60. Cronbach's Alpha in our sample was 0.94. The reliability and validity of the BDI have been confirmed in clinical and community samples of adolescents.
Anxiety symptoms: Symptoms of anxiety were assessed using the Beck Anxiety Inventory (BAI), a 21-item self-report questionnaire. Responses to each item range from 0 to 3 with scores ranging from 0 to 63 with higher scores indicating increased levels of anxiety.
Cronbach's Alpha in our sample was 0.93.
Wellbeing: Wellbeing was assessed using the Warwick Edinburgh Mental Wellbeing Scale (WEMWS), which measures positive psychological wellbeing through a 14 item self-report questionnaire. Responses to each item range from 1 to 5 with overall scores ranging from 14 to 70 where 70 represents the best possible level of wellbeing. Cronbach's alpha in our sample was 0.83, indicating good internal reliability.

Sleep
Sleep was measured using an adapted form of the Pittsburgh Sleep Quality Index (Buysse, Reynolds, Monk, Berman & Kupfer, 1989) which has been validated for use in adolescents (Raniti, Waloszek, Schwartz, Allen & Trinder, 2018). Participants were asked to report how many hours they spent asleep on a typical school night and to rate their quality of sleep as either "Very Good", "Fairly Good", "Fairly Bad" or "Very Bad".

Physical activity
Habitual physical activity was assessed via a modified version of the Take PART questionnaire by measuring the number of days during the past 14 that participants had accumulated 60 minutes of moderate-to-vigorous physical activity (MVPA)). The survey item assessing physical activity was as follows: "During a typical 2-week period, on how many days were you physically active for a total of at least 60 minutes? For each day, add up all the time you spent in physical activity like walking, riding a bicycle etc. Count up the days with at least 60 minutes of physical activity in a typical 2-week period." Responses ranged from 0 to 14 days.
Based on their reported frequency of physical activity, participants were categorised as Least Active (60 minutes or more of activity on 0-3 days in the past 14), Somewhat Active (60 minutes or more of activity on 4-7 days in past 14), or Most Active (60 minutes or more of activity on 8-14 days of the past 14. A further sub-group was also created who reported meeting the daily physical activity guidelines on all 14 days in a typical 2-week period (Sufficiently Active according to WHO guidelines).

Statistical analyses
To examine the associations between self-reported hours of sleep and mental health variables a mixed linear regression model with fixed effects for gender and physical activity, and random effects for year was fitted. The coefficients from this model were used to estimate curves illustrating associations between subjective sleep and each of the mental health measures (figure 2). Post-hoc between-group comparisons were carried out using Tukey's HSD and Bonferroni correction to account for multiple testing. Analyses were conducted in R (R Core Team, 2014) and figures were produced using the package ggplot2 (Wickham, 2009).

Figure 1: Percentage of Males And Females Sleeping 8-10 Hours Per Night By Year
A higher percentage of participants met the guidelines of 8-10 hours of sleep per night in younger year groups and this was found to decline as adolescents progressed through school, with the exception of males in transition year. A higher percentage of males were also found to have meet sleep duration guidelines in all year groups with the exception of 6 th year.  Table 1 shows the percentage of males and females in each category of sleep. 53% of females and 58% of males met the guideline of 8-10 hours per night. More females than males were found to sleep 5 to 6 (16% v 12%) and 6 to 8 hours per night (28% v 25%) respectively.
One-way ANOVA found significant differences between self-reported quality of sleep and

Self-Reported Sleep and Physical Activity
In our sample, 63% of males and 63.7% of females who met physical activity recommendations also met the recommended 8-10 hours of sleep per night and can be seen in

Discussion
This cross-sectional study sought to identify the percentage of male and female Irish adolescents who met the US National Sleep Foundation's recommendations of "8-10 hours per night" (Hirshkowitz et al., 2015). The authors also sought to the impact that self-reported sleep has on mental health outcomes, particularly wellbeing and symptoms of anxiety and depression and how levels of physical activity may contribute to this. On average, 55.5% of Irish adolescents achieved good sleep hygiene with more males (58%) reporting 8-10 hours of sleep than females (53%) as can be seen in table 1. This is slightly more than a previous investigation of sleep in Irish adolescents who found that 52% of males achieved 8-10 hours While consistent with previous research in Ireland, a larger percentage of males meeting sleep recommendations is in contrast to international research who found that males sleep less and go to bed later than females (Keyes, Maslowsky, Hamilton & Schulenberg, 2015). Irish adolescents are slightly above the norm in terms of good sleep hygiene but still below the 86% of Flemish Belgian adolescents who sleep 8-10 hours per night, although they are well ahead of other countries such as Poland (32%), Greece (35%), and Slovenia (38%) as was reported by the Health Behaviour In School-Aged Children's Study (Gariepy et al., 2020). Sleep patterns changing with age is in line with previous studies (Gradisar, Gardner & Dohnt, 2011;Olds et al., 2010). Older adolescents have consistently been found to sleep less, go to bed later and experience greater social jetlag than younger adolescents (Gariepy et al., 2020).
These changes reflect the normative developmental course of the circadian clock, which shifts toward evening-ness around puberty, peaks at around age 16 years, and shifts back in early adulthood (Randler, Fabl & Kalb, 2017). Increased schoolwork along with greater use of social media and electronic devices add to the biological tendency of older adolescents to fall asleep at a later time. Older adolescents are also afforded greater autonomy by their parents in terms of selecting sleep times (Pyper, Harrington & Manson, 2017). It must be noted that sleep patterns were examined across year group and not pubertal development. Changes in adolescent sleep patterns have been attributed to hormonal and physiological changes related to puberty more than age (Sadeh, Dahl, Shahar & Rosenblat-Stein, 2009) and so could be explored further in future research.
As reported by Murphy, Sweeney and McGrane (2020), 4 out of 10 Irish adolescents suffer from elevated symptoms of anxiety and depression. The lowest symptoms of anxiety and depression found in this sample were in adolescents who reported good sleep hygiene, with incremental increases in symptoms as sleep decreased. This inverse association between sleep and mental ill-health has also been found in objective measures of sleep such as polysomnography (Moore, Bardwell, Ancoli-Israel & Dimsdale, 2001). As can be seen in figure   2, 9-10 hours of sleep appears to have the strongest association with mental wellbeing and reduced symptoms of anxiety and depression. Prospective studies have previously demonstrated that sleep problems and/or sleep deprivation increase the risk for subsequent mental/emotional dysfunction in adolescents (Gregory & O'Connor, 2002) with shorter sleep increasing the risk for depressive symptoms and low self-esteem both concurrently and over time (Fredriksen, Rhodes, Reddy & Way, 2004). Studies looking at the associations between sleep and psychosocial outcomes have largely focused on sleep restriction and adolescents with sleep problems. One such study, which examined elements of psychological and social functioning such as self-esteem, depression, perceived mental health, life satisfaction, relations with parents and social support found that baseline insomnia increased the risk for all subsequent psychosocial outcomes in a dose response manner (Roberts et al., 2009). These associations were maintained for self-esteem, depression and social support even after adjusting for age, gender, parental education and concurrent insomnia at follow up with severe insomnia increasing all risks by nearly twofold. Low life satisfaction and low levels of perceived mental health have consistently been found in multivariate analyses (Shochat, Cohen-Zion & Tzischinsky, 2014). Temporal associations between inadequate sleep and mental ill-health were explored through a series of retrospective interviews with over 1000 Japanese adolescents (Kaneita et al., 2009). Over 50% of adolescents with insomnia had a comorbid psychiatric disorder with anxiety increasing the risk for insomnia, whereas insomnia increased the risk for depression. This suggests a bi-directional relationship between inadequate sleep and mental ill-health which was previously suggested as a possible mechanism by Lubans et al (2016), in particular how participation in physical activity may improve sleep duration, sleep efficiency (Stone, Stevens & Faulkner, 2013), sleep onset latency (Lang et al., 2013) and reduced sleepiness (Gaina et al., 2007). Physical activity has also been shown to positively influence self-regulation and coping skills that have subsequent implications for mental health (Lubans et al., 2016).
The associations between self-reported sleep quantity and symptoms of anxiety and depression appear to mirror the associations with wellbeing. 9-10 hours also seemed to be the "sweet-spot" for our sample of adolescents. Research investigating positive psychological states and sleep has increased alongside the growth of positive psychology as a construct in its own right. One such investigation, found that aspects of eudaimonic wellbeing, including purpose in life, environmental mastery, and positive relationships, were positively associated with good sleep in a cohort of older adults (Ryff, Singer & Dienberg, 2004). The authors observed that the environmental mastery component of eudaimonic wellbeing was positively associated with sleep duration and the amount of rapid eye movement (REM) sleep, and negatively with delay to the first onset of REM. Steptoe et al (2008) found that positive affect and eudaimonic wellbeing were associated with fewer sleep problems independently of age, gender, household income, employment status and self-rated health. The causal influence of sleep on wellbeing, or vice versa, is unclear from the current study. Steptoe et al (2008) found that sleep problems were commonly reported by participants experiencing high financial strain, high psychological distress, and more negative social interactions, all of which have a negative impact on wellbeing. They suggest that happier individuals, with a greater sense of purpose and positive orientation, may be protected in part from the adverse impact of stress and adversity, therefore less likely to suffer from sleep disturbances. It is also likely that poor sleep both engenders lower positive affect and reduced eudaimonic wellbeing, and that positive psychological states lead to better sleep. This has been reinforced by recent findings from a web-based survey involving 1165 participants that suggests people who sleep poorly are more likely to report negative emotions than persons who sleep well and that worse-thanusual sleep is followed by more negative emotions the day after (Narmandakh, Oldehinkel, Masselink, de Jonge & Roest, 2021).
Accumulating evidence suggests a number of health benefits for adolescents, both independently and combined, from increased physical activity and sleep (Saunders et al., 2016). Both physical activity and sleep have demonstrated significant inverse associations with depression, even after controlling for age, gender and socioeconomic backgrounds. The current study has demonstrated that higher volumes of physical activity were positively associated with more hours of self-reported sleep and higher self-reported sleep quality. A previous longitudinal study found that depressive symptoms, physical activity and sleep disturbances were significantly interrelated over time with initial levels of depressive symptoms predicting increases in sleep disturbance and initial levels of sleep disturbance predicting decreases in physical activity (Raudsepp & Vink, 2019). Two main hypotheses have been suggested as to the mechanisms through which sleep is positively impacted by physical activity. Higher levels of activity may produce physiological changes that are favourable to sleep regulation. An experimental trial in young adolescents found that exposure to highintensity exercise resulted in a significant rise in the proportion of slow wave sleep, greater sleep efficiency and shorter sleep onset latency (Dworak et al., 2008) while another trial in older adolescents found three consecutive weeks of morning running led to increased objective measures of total sleep and decreased objective sleep onset latency (Kalak et al., 2012). The second hypothesis suggests the sleep-promoting effects of increased physical activity are mediated by psychological functioning (Biddle & Mutrie, 2008), such as reduced symptoms of anxiety, depression and stress through physical activity (Dunn, Trivedi, Kampert, Clark & Chambliss, 2005).

Limitations
While the large representative sample was a strength of the study, the use of self-report