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Pleiotropic Properties of Valsartan: Do They Result from the Antiglycooxidant Activity? Literature Review and In Vitro Study

Valsartan belongs to angiotensin II type 1 (AT1) receptor blockers (ARB) used in cardiovascular diseases like heart failure and hypertension. Except for its AT1-antagonism, another mechanism of drug action has been suggested in recent research. One of the supposed actions refers to the positive impact on redox balance and reducing protein glycation. Our study is aimed at assessing the antiglycooxidant properties of valsartan in an in vitro model of oxidized bovine serum albumin (BSA). Glucose, fructose, ribose, glyoxal (GO), methylglyoxal (MGO), and chloramine T were used as glycation or oxidation agents. Protein oxidation products (total thiols, protein carbonyls (PC), and advanced oxidation protein products (AOPP)), glycooxidation products (tryptophan, kynurenine, N-formylkynurenine, and dityrosine), glycation products (amyloid-β structure, fructosamine, and advanced glycation end products (AGE)), and albumin antioxidant activity (total antioxidant capacity (TAC), DPPH assay, and ferric reducing antioxidant power (FRAP)) were measured in each sample. In the presence of valsartan, concentrations of protein oxidation and glycation products were significantly lower comparing to control. Moreover, albumin antioxidant activity was significantly higher in those samples. The drug's action was comparable to renowned antiglycation agents and antioxidants, e.g., aminoguanidine, metformin, Trolox, N-acetylcysteine, or alpha-lipoic acid. The conducted experiment proves that valsartan can ameliorate protein glycation and oxidation in vitro in various conditions. Available animal and clinical studies uphold this statement, but further research is needed to confirm it, as reduction of protein oxidation and glycation may prevent cardiovascular disease development.


Introduction
The formation of free radicals is an inevitable consequence of aerobic metabolism. As long as physiological mechanisms manage to eliminate reactive oxygen species (ROS), their adverse action on structural proteins, enzymes, membrane lipids, or nucleic acids may remain unseen [1]. However, the generation of ROS may exceed the capability of the organism to neutralize them. This situation is called redox imbalance and leads to oxidative damage to cellular biomolecules [2]. Indeed, ROS are not only proven to play a crucial role in many physiological processes [3,4], but they are also a vital factor in the pathogenesis of many diseases like obesity [5,6], insulin resistance [7,8], hypertension [9,10], and chronic heart failure [11,12].
ROS's role in the pathogenesis of hypertension is complicated and involves many different biochemical mechanisms [13][14][15]. ROS produced by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases are responsible for nitric oxide (NO) depletion caused by its interaction with superoxide radical anions (O 2 -• ). This results in decreased endothelial nitric oxide synthase (eNOS) activity and leads to endothelial dysfunction, which is considered one of the essential pathogenetic factors [16,17]. What is more, oxygen-free radicals can act on redox-sensitive genes in vascular smooth muscle cells and promote their mitogenic phenotype, stimulate fibrosis by inhibiting matrix metalloproteinases, or ameliorate an arterial wall calcification by activation of specific bone morphogenetic proteins (BMP). As an effect of this remodeling, stiffness of the vessel and peripheral resistance increase [9].
It is also reported that glycation of vascular proteins plays a vital role in hypertension development [5,13,14]. Interestingly, higher circulating levels of advanced glycation end products (AGE) correlate with increased arterial stiffness [18,19]. Indeed, AGE accumulation in blood vessels impairs endothelial function by decreasing eNOS activity and reducing NO bioavailability [14,20]. AGE also combine with a specific receptor (RAGE) to activate the transcription factor NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cell). This not only stimulates ROS production but also activates many proinflammatory genes such as interleukins (IL-1b, IL-2, IL-6, and IL-8), adhesion molecules (vascular cell adhesion protein 1 (VCAM-1), intercellular cell adhesion protein 1 (ICAM-1)), growth and differentiation factors (vascular endothelial growth factor (VEGF), and transforming growth factor β2 (TGF-β2)) [20,21]. Under these conditions, ROS production is intensified, which increases the already existing oxidative stress. Nevertheless, AGE also affect the activation of monocytes that overexpress the CD36 sweeping receptor. In this way, lipoproteins foam cells are formed, and atherosclerosis development is accelerated [20,21]. Therefore, the use of antioxidants in cardiovascular diseases may have several positive effects [22].
According to recent studies, the drug which may potentially exhibit antioxidant and antiglycation activity is valsartan. Valsartan, (2S)-3-methyl-2-[pentanoyl-[[4-[2-(2Htetrazole-5-yl)phenyl]phenyl]methyl]amino]butanoic acid, is a lipophilic, nonpeptide, tetrazole derivative, a selective antagonist of angiotensin II type 1 receptor (AT 1 ), which is widely used in many cardiovascular conditions like hypertension or chronic heart failure. The drug is administered orally, is characterized by rapid absorption and low bioavailability (25%), and is almost entirely bound to plasma proteins (94-97%) [23,24]. The hepatic products of the metabolism of valsartan are mainly excreted with bile [25]. In comparison to other antihypertensives, valsartan is well tolerated and deprived of many side effects, like cough or angioedema, characteristic for angiotensin-converting enzyme inhibitors (ACEI). An occurrence of valsartan side effects in clinical studies was comparable to placebo, which confirms the drug's safety [26]. Moreover, it has been proven to act nephroprotective in patients with diabetes mellitus and chronic renal failure and significantly reduce albuminuria [27,28]. Some reports are claiming that valsartan may present additional mechanisms of action besides its AT 1 receptor antagonism. It was proven that patients treated with valsartan presented less urinary oxidative stress markers [29]. Similar outcomes were observed in mice where valsartan diminishes oxidative damage and acts nephroprotective [30].
However, it is still uncertain whether valsartan has antioxidant and antiglycation activity. Indeed, the impact of various glycating agents on protein oxidation/glycation in in vitro or in vivo models has not yet been evaluated. The effect of valsartan action was also not compared to other substances with recognized antioxidant properties. This is particularly important because confirmation of the antiglycooxidant activity of valsartan may result in a revision of the guidelines for the use of the drug in cardiovascular disease and diabetes, making it the first-line medication in patients with enhanced protein glycation. For this reason, we conducted a study to assess valsartan's effect on protein oxidation, glycation, and total antioxidant activity in in vitro model of oxidized bovine serum albumin (BSA). Either oxidizing (chloramine T) or glycating agents (glucose, fructose, ribose, glyoxal, and methylglyoxal), as well as antioxidants (Trolox, N-acetylcysteine, lipoic acid, and captopril) and protein glycation inhibitors (aminoguanidine and metformin), were used to compare the results of antiglycooxidant capabilities of valsartan [31][32][33][34][35]. We assessed the concentrations of protein oxidation products (total thiols, protein carbonyls, and advanced oxidation protein products (AOPP)), glycooxidation products (tryptophan, kynurenine, N-formylkynurenine, and dityrosine), levels of albumin glycation products (amyloid-β structure, fructosamine, and advanced glycation end products (AGE)), and antioxidant potential of albumin (total antioxidant capacity (TAC), DPPH assay, and ferric reducing antioxidant power (FRAP)). We have also made a detailed literature review on the antiglycooxidant properties of valsartan.

Reagents and Equipment.
All reagents (analytical grade) were purchased from Sigma-Aldrich (Nümbrecht, Germany, or Saint Louis, MO, USA). Solutions were sterilized by filtration through 0.2 mm membrane filters directly before use. The fluorescence and absorbance were evaluated using a microplate reader (M200 PRO multimode microplate reader; Tecan Group Ltd., Männedorf, Switzerland).
Chloramine T was used as an oxidation agent. 0.09 mM BSA and 1 mM valsartan were incubated with 20 mM chloramine T for 60 minutes [37,38]. 2 Oxidative Medicine and Cellular Longevity As glycating agents, sugars (glucose (Glu), fructose (Fru), and ribose (Rib)) and aldehydes (glyoxal (GO), and methylglyoxal (MGO)) were used. To assess the additives' effect on protein glycation, 0.09 mM BSA and 1 mM valsartan were incubated with 0.5 M Glu, Fru, and Rib for 6 days or 2.5 mM GO and MGO for 12 hours [31,32,39]. GO and MGO were used within a month after delivery, and solutions were prepared immediately before use [34].
All experiments were performed three times, each time in duplicate.

Protein Oxidation Products.
Total thiols were detected using Ellman's reagent by the colorimetric method [42]. The absorbance was measured at 412 nm wavelength. Thiol groups' content was established based on a standard curve for N-acetylcysteine (NAC) [42].
The carbonyl groups' reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) was performed to examine protein carbonyl concentration in proteins that underwent oxidative damage. The absorbance of the products of this reaction was evaluated colorimetrically at 355 nm wavelength [43].
The concentration of advanced oxidation protein products was measured by spectrophotometric detection. 200 μL of examined solutions diluted with PBS in 1 : 5 ratio (v : v), chloramine T standard solutions (0-100 μmol/L), and 200 μL of blank PBS were placed in 96-well microplates. Then, 20 μL of acetic acid and 10 μL of 1.16 M potassium iodide were added to wells. Immediately after that, the absorbance was measured at 340 nm wavelength concerning blank (200 μL PBS, 20 μL acetic acid, and 10 μL potassium iodide). Chloramine T solutions presented linear absorbance in the range of 0-100 μmol/L [44].
2.5. Protein Glycation Products. The assay was performed to measure fluorescence emitted when amyloid fibrils or oligomers are bound to amyloid-β structure. 10 μL of Thioflavin T and 90 μL of samples were mixed and transferred to a microplate; then, the fluorescence was assessed at 385/485 nm [46,47].
All examined samples were diluted with 0.1 M sulfuric acid (1 : 5, v : v) [18]. Then, the content of advanced glycation end products (AGE) was measured using the spectrophotometric method at 440/370 nm at a 96-well microplate reader [44,49]. AGE content was also analyzed with the ELISA method (UCSN, Life Science, Wuhan, China).
2.6. Antioxidant Activity. TAC of each sample was measured according to Erel's method. For this purpose, 2,2-azinobis(3ethylbenzene-thiazoline-6-sulfonate) (ABTS) radical cation decolorization assay was used. ABTS + was obtained by reacting ABTS with potassium persulfate and incubated for 12 hours at room temperature. After mixing 10 μL of samples with 1 mL of ABTS + , the absorbance readings were taken at 735 nm. Results of decolorization were linear with increasing Trolox concentrations [50,51].
The determination of free radicals scavenging activity was performed according to the Brand-Williams method. 10 μL of each sample and 390 μL methanolic diluted DPPH were mixed and placed on a 96-well microplate. The solutions were incubated in darkness at room temperature for 30 minutes. The absorbance was measured at 515 nm [52,53].
The ferric reducing antioxidant power of each sample was measured following the Benzie and Strain method. FRAP reagent was prepared by mixing 25 mL acetate buffer, 2.5 mL FeCl 3 ·6H 2 O solution, and 2.5 mL TPTZ solution and warming the solution to 37°C. 10 μL of sample and 300 μL of FRAP reagent were diluted with 30 μL of water and transferred to 96-wells microplate. The change of absorbance was calculated for each sample and related to the absorbance of Fe II standard solution [54].
2.7. Statistical Analysis. The statistical analysis was conducted using GraphPad Prism 8.3.0 (GraphPad Software, La Jolla, CA, USA). The results were expressed as a percentage of the corresponding control values (BSA + glycating/oxidizing agent). Differences between groups were assessed by one-way ANOVA followed by Tukey's post hoc test for multiple comparisons. p < 0:05 was considered statistically significant. Multiplicity adjusted p value was also calculated.
Amyloid-β structure, fructosamine, and AGE contents were significantly higher in BSA incubated with glucose than BSA alone. All reviewed substances caused a significant decrease in parameters above (valsartan: 89%, 56%, and 73% vs. control, respectively), excluding sample with metformin in AGE measurement (Table 1).

The Impact of Valsartan and Other Additives on Protein Oxidation Products, Glycooxidation Products and Glycation
Products as well as Antioxidant Activity in Fructose-(Fru-) Induced Albumin Glycation. Fructose-induced protein oxidation resulted in increased PC and AOPP levels and decreased total thiols level compared to BSA alone. All investigated agents caused a significant decrease in concentrations of PC and AOPP (valsartan: 81%, 58% vs. control, respectively), excluding BSA + Fru + metformin in AOPP concentration. Moreover, the addition of valsartan (105% vs. control), NAC (114% vs. control), ALA (107% vs. control), and aminoguanidine (105% vs. control) led to a higher concentration of total thiols compared to control ( Table 2).
The results of protein glycooxidation products measurement showed significantly higher concentrations of kynurenine, N-formylkynurenine, and dityrosine in BSA + Fru comparing to BSA alone. However, the content of tryptophan decreased after fructose was added. In samples with all reviewed inhibitors, significantly lower kynurenine concentrations, N-formylkynurenine, and dityrosine (valsartan: 67%, 57%, and 65% vs. control, respectively) were observed (Table 2).
Moreover, the presence of fructose in the BSA solution resulted in greater concentrations of measured glycation products. The analysis showed significantly lower levels of amyloid-β structure, fructosamine, and AGE in samples with the addition of all investigated protein glycation and oxida-tion inhibitors (valsartan: 88%, 53%, and 84% vs. control, respectively) comparing to control.

The Impact of Valsartan and Other Additives on Protein
Oxidation Products, Glycooxidation Products, and Glycation Products as well as Antioxidant Activity in Ribose-(Rib-) Induced Albumin Glycation. The addition of ribose to BSA solution resulted in lower total thiols level and higher PC and AOPP levels. All reviewed agents (valsartan: 68%, 76% vs. control, respectively) caused a significant decrease in PC and AOPP contents. Moreover, after inhibitors (valsartan: 105% vs. control) were added to BSA + Rib, statistically higher total thiols were noticed (Table 3).
Regarding glycation products, the presence of ribose in BSA resulted in greater amyloid-β structure, fructosamine, and AGE compared to BSA alone. Furthermore, decreased levels of these parameters were noticed in samples with BSA + Rib and all investigated inhibitors (valsartan: 96%, 58%, and 66% vs. control, respectively), excepting metformin in amyloid-β structure level (Table 3).
The presence of glyoxal in BSA solution resulted in higher glycation product content. Moreover, decreased amyloid-β structure, fructosamine, and AGE concentrations in all investigated samples versus control were observed (valsartan: 71%, 87%, and 52% vs. control, respectively), excepting sample with metformin (Table 4).
Regarding total antioxidant potential assays, the BSA + GO sample was characterized by significantly lower TAC, DPPH, and FRAP parameters than the BSA alone sample. Significantly greater TAC and DPPH values in all investigated samples, excluding DPPH in a sample with captopril, were observed. The addition of valsartan (118% vs. control), NAC (117% vs. control), ALA (107% vs. control), and aminoguanidine (124% vs. control) caused an increase of FRAP compared to control. On the other hand, the presence of captopril (88% vs. control) and metformin (78% vs. control) led to significantly lower FRAP value (Table 4).
The presence of methylglyoxal resulted in a significant decrease in tryptophan concentration and an increase of kynurenine, N-formylkynurenine, and dityrosine concentrations. Significantly higher tryptophan levels were observed after inhibitors were added (valsartan: 107% vs. control). The majority of investigated substances (valsartan: 87%, 74%, and 72% vs. control, respectively) caused a significant decrease of kynurenine, N-formylkynurenine, and dityrosine concentrations (except ALA and metformin) ( Table 5).
Furthermore, a decrease of amyloid-β structure, fructosamine, and AGE concentrations was observed due to methylglyoxal addition to the BSA sample. All used inhibitors (valsartan: 66%, 38%, and 56% vs. control, respectively) were responsible for the decrease of given parameters (Table 5).
Methylglyoxal was also the cause of decreased TAC, DPPH, and FRAP values compared to BSA alone. All the investigated samples (valsartan: 144%, 113%, and 132% vs. control, respectively), except metformin, were characterized by significantly higher TAC levels in comparison to control (Table 5).

The Impact of Valsartan and Other Additives on Protein Oxidation Products, Glycooxidation Products, and Glycation
Products as well as Antioxidant Activity in Chloramine T-Induced Albumin Glycation. Markers of oxidative damage (PC and AOPP) were significantly greater in the presence of chloramine T compared to BSA alone. In all investigated samples (valsartan: 71%, 56% vs. control, respectively), significantly decreased levels of the parameters above versus control were observed. In addition, all used inhibitors (valsartan: 110% vs. control) caused a significant increase of total thiols level ( Table 6).
Glycooxidation of proteins induced by chloramine T resulted in greater kynurenine concentrations, N-formylkynurenine, and dityrosine together with a lower concentration of tryptophan. The addition of valsartan (89%, 69% vs. control, respectively) and other substances led to decreased amounts of given parameters compared to control. In contrast, all the additives (excluding aminoguanidine) caused higher tryptophan concentrations in comparison to control ( Table 6).
Chloramine T-induced glycation resulted in significantly higher levels of amyloid-β structure, fructosamine, and AGE. The analysis showed significantly lower concentrations of fructosamine and AGE when all investigated inhibitors were added. Moreover, a statistically decreased amount of amyloid-β structure in samples with valsartan (95% vs. control), NAC (87% vs. control), and captopril (95% vs. control) was noticed. However, the BSA + chloramine T + metformin sample presented a significantly higher amyloid-β structure than the control (Table 6).
Significantly decreased antioxidant activity markers were observed when BSA was incubated with chloramine T. The addition of valsartan (106%, 119%, and 133% vs. control, respectively) and other investigated agents, except metformin, caused a significant increase of TAC, DPPH, and FRAP value ( Table 6).

Discussion
Cardiovascular diseases (CVD) are the most common cause of death worldwide. Many factors are involved in the pathogenesis of such diseases, but the role of oxidative stress seems to be crucial [55][56][57][58]. Oxidative stress, which is defined as excessive production of reactive oxygen species (ROS) and disproportion of oxidants over antioxidants, causes myocardial remodeling by activating hypertrophy-signaling kinases and stimulating cardiac fibroblasts to proliferate. ROS also affect myocardial calcium handling and lead to cellular dysfunction by inducing changes in intracellular pathways [59,60]. Moreover, in dysfunctional myocardium, enhanced ROS production is observed, and the antioxidant mechanism's exhaustion leads to disease progression [61,62]. Oxidative stress likewise participates in the pathogenesis of hypertension. ROS decrease the availability of nitric oxide (NO), causing vasoconstriction and lead to modification of low-density lipoprotein (LDL), increasing its uptake by 9 Oxidative Medicine and Cellular Longevity

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Oxidative Medicine and Cellular Longevity

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Oxidative Medicine and Cellular Longevity macrophages. As a result, the so-called foam cells are formed. Those cells take a very important part in atherosclerosis's pathogenesis, which is a well-known factor of hypertension and other diseases, such as coronary artery disease [16]. Oxidative stress is also a proven link between diabetes mellitus (DM) and CVD [63]. Furthermore, in patients diagnosed with DM, elevated levels of advanced glycation end products (AGE) are observed. This, along with oxidative stress, may explain the pathogenesis of cardiovascular complications of DM [64][65][66][67]. AGE are also a proven factor leading to heart failure and other CVD [11,[68][69][70][71].
Valsartan is a potent and specific angiotensin II receptor antagonist used to treat hypertension and chronic heart failure. Many clinical studies confirm the effectiveness of the drug, especially in combination with sacubitril [72,73]. What is interesting, it is also proven that the therapy with valsartan significantly increases the long-term quality of life in patients with chronic heart failure [74], which is a result of biochemical, echocardiographic, and clinical improvements [75]. Simultaneously, the drug remains safe in patients with many comorbidities, especially in chronic kidney disease [76]. In patients with hypertension, the use of valsartan is also associated with the reduction of the risk of organ complications, including left ventricular hypertrophy [77,78]. Due to the valsartan's pleiotropic properties, it is believed that the mechanism of drug action is not fully understood.
The research about the antioxidant properties of valsartan is especially limited. Nevertheless, the majority of accessible papers uphold the hypothesis of the drug's positive impact on the redox homeostasis in vivo [29,[79][80][81][82][83][84][85][86][87][88][89][90] (Table 7). Experiments conducted on animals show a statistically significant decrease in oxidative stress parameters and inflammatory and cellular damage markers. Valsartan also causes an increase in antioxidant enzymes' activities and decrease concentrations of adhesive and chemotactic factors. What is important, results of clinical studies remain consistent with the animal model results (Table 7). However, there are no data on the antiglycation activity of valsartan. Only Komiya et al. revealed a statistically significant decrease in blood AGE concentration in diabetic and hypertensive patients treated with valsartan [86]. This may suggest a potential antiglycation role, but there is no more available evidence to support this statement.
Despite all the facts that stand for valsartan as the antioxidant potential, some limitations are worth mentioning regarding this research. Available clinical studies were conducted in relatively small groups of patients, making it more challenging to perform a reliable statistical prediction. In some studies, patients continued the treatment with previously prescribed drugs before administering valsartan. It is unclear if these drugs had no known impact on cellular redox balance and interfere with the trial's results. There is also even more limited information regarding the influence of valsartan on protein glycooxidation. Thus, it is necessary to evaluate the effect of valsartan on protein oxidative damage by measuring the oxidation rate of thiol and carbonyl groups, aromatic amino acid residues, or assessing the extent of early and late protein glycation products.
Our study is the first to assess valsartan's antiglycation properties with respect to various glycating and oxidizing agents. Using an in vitro model, we have shown that valsartan strengthens the antioxidant barrier and inhibits oxidation and albumin glycation comparable to recognized ROS scavengers (Trolox, N-acetylcysteine, lipoic acid, and captopril) and protein glycation inhibitors (aminoguanidine and metformin). Considering the key role of oxidative stress in CVD pathology, valsartan's pleiotropic activity may result from its antioxidant and antiglycation properties.
Albumin, the main plasma protein, has a crucial role in the human organism. It is responsible for the transport of various substances such as hormones or drugs and maintaining blood pH or colloid-oncotic pressure. Albumin can also bind transition metal ions which explains its antioxidative properties [94][95][96]. Due to its high plasma concentration, long half-life, and high content of arginine, cysteine, and lysine, albumin can be easily glycated and oxidized in vivo [97,98]. Albumin glycation involves nonenzymatic addition of reducing sugar to its amino groups. This process is subdivided into several phases. During the early ones are formed, the first Maillard reaction produces the Schiff base and the Amadori products. At the final stage, the advanced glycation end products (AGEs) are produced-including carboxymethyl lysine (CML), furyl-furanyl-imidazole (FFI), pentosidine, and pyralin [68,70,82,98]. The glycation process occurs simultaneously with oxidation, by which they are collectively referred to as glycooxidation. The final products of protein oxidation are advanced oxidation protein products (AOPP). AOPPs originate from the accumulation of oxidized residue of arginine, cysteine, dityrosine, and tryptophan [69,99,100].
Our research demonstrated that valsartan ameliorates protein oxidation (↑total thiols, ↓PC, and ↓AOPP), reduces albumin glycooxidative damage (↑tryptophan, ↓kynurenine, ↓N-formylkynurenine, and ↓dityrosine), prevents glycation (↓amyloid-beta structure, ↓fructosamine, ↓and AGE), and enhances the antioxidant activity of albumin (↑TAC, ↑DPPH, and ↑FRAP). These results are similar in every investigated glycating or oxidizing agent combined with the drug. However, some differences in the drug's action are distinguishable between sugars and aldehydes. Generally, the antiglycooxidant properties of valsartan are more marked in samples containing GO or MGO and valsartan, where the parameters decrease or increase even more in comparison to the sugars samples (e.g., AGE content is lower in BSA + GO/MGO + valsartan than in BSA + Glu/Fru/Rib + valsartan). Nevertheless, it is worth mentioning that the oxidation and glycation substances used in the research were at greater concentrations than physiological levels that might impact the experiment's outcome [31,32]. However, our model is kinetically validated and allows us to evaluate unknown substances' antiglycooxidative properties rapidly [31,34]. There were no prominent differences between used sugars, as given results in their samples were comparable. Interestingly, valsartan exhibited antioxidant and antiglycooxidant properties in chloramine T presence, but glycation inhibition was not so potent as in the case of sugars or aldehydes.

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Oxidative Medicine and Cellular Longevity   14 Oxidative Medicine and Cellular Longevity Although our study does not explain it, valsartan's antiglycooxidant properties may be due to the molecule's chemical structure. It can be speculated that the -NH 2 group in valsartan competes with the amino residues of proteins for the attachment of reactive carbonyl groups. Thus, it protects the lysine and arginine residues of proteins from their glycooxidative modifications. Therefore, future investigations are necessary to validate conclusions that can be drawn from this study. The next phase of research is the in vivo analysis of antiglycooxidant activity and optimal dosage of valsartan in 15 Oxidative Medicine and Cellular Longevity animal and human models. However, the pleiotropic properties of valsartan may also be due to its other in vivo activities. Angiotensin II is a major effector peptide of the renin-angiotensin-aldosterone system, and it is generated from angiotensin I by the angiotensin-converting enzyme. Moreover, angiotensin II by acting on the AT 1 receptor stimulates NADPH oxidase activation (Nox) and increases expression of Nox subunits leading to ROS overproduction [101]. Importantly, blockade of the reninangiotensin system with blockers of AT 1 receptors (ARBs) (such as valsartan) can reduce oxidative stress due to inhibition of the processes mentioned above. It was also shown that valsartan causes increased SOD expression, which transforms superoxide radicals into hydrogen peroxide and oxygen in a disproportionate reaction [102]. It is also speculated that ARBs can decrease protein oxidation via free radical scavenging and transition metal chelation [103].
Inhibition of protein glycation by valsartan may be of particular importance in patients with diabetic cardiomyopathy. AGE generated under hyperglycemic conditions increase ROS overproduction, which impairs the activity of ion pumps and mitochondria, disrupts the transport of calcium ions between cellular compartments, and initiates apoptosis. Extracellular matrix collagen also undergoes glycation, increasing cardiac stiffness and decreasing diastolic capacity and nerve impulse conduction velocity [61,62]. It can be speculated that valsartan may inhibit the synthesis of AGE by directly neutralizing reactive dicarbonyl compounds by combining drug amino group with the α-dicarbonyl group of methylglyoxal. Nevertheless, these hypotheses require confirmation in further studies.
Overall, conducted research showed that valsartan can reduce oxidation and glycation damage and improve the albumin's antioxidant properties. The valsartan's activity was recorded against various oxidizing and glycating agents. What is more, the drug's action is comparable to many renowned antioxidants or is even more pronounced. The pleiotropic properties of valsartan may be due to its antiglycooxidant activity. Inhibition of protein glycation/oxidation in patients with CVD and DM may be crucial, given the significant contribution of oxidative and carbonyl stress to the development of cardiometabolic complications. Further studies considering this subject may be revolutionary for the treatment of cardiovascular diseases. Confirmation of the antiglycating effect of valsartan in human studies could result in a revision of clinical guidelines for the use of hypotensive drugs. Valsartan could be a first-line medicine in patients with heart disease and diabetes. Modifications to the chemical structure of valsartan should also be considered. The introduction of -NH 2 groups (as in polyamide compounds) could increase the antiglycation activity's potency.

Data Availability
The article contains complete data used to support the findings of this study.