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PCR versus ELISA in diagnosis of human toxoplasmosis in Jeddah, Saudi Arabia.

In this study, polymerase chain reaction (PCR) was compared with detection of specific antibodies to Toxoplasma by ELISA. A total of 70 blood samples from patients with different clinical features were tested, 43/70 (61.4%) were serologically Toxoplasma-IgG positive meanwhile only 16/43 (37.2%) were approved to be positive by PCR. A total of 22 samples (31.6%) were positive by PCR, but only 16 of them were positive by ELISA. So, Tox-IgG indicated catching Toxoplasma infection but not enough with immune modulation to determine acute case, while PCR besides, being valuable in diagnosis showed Toxoplasma parasitemia.