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Microfluidic sterilization in a high temperature/pressure continuous flowing chip

This paper reports the design, working principle, fabrication and the structure of a high temperature/pressure continuous flowing chip. The chip can reach a high pressure of 500kPa and temperature of 150°C, which can be used for portable cell culture system. The bacterial sterilization experiments are conducted with 108/ml E.coli and 107/ml Staphylococcus aureus flowing through the sterilization chip. The results show a sharp decrease of population proving the chip has an efficient and universal sterilization effects.