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[Production and characterization of a group of monoclonal antibodies to human transitional cell carcinoma].

A panel of specific mouse monoclonal antibodies, BIU-H4, BIU-H6, BIU-H9, was obtained by immunization with BIU-87 cells (a transitional cell carcinoma cell line of the bladder), which was blocked by the serum of mouse anti-human lymphocyte. Detailed descriptions were given concerning their reactivity patterns by ABC-ELISA and IIF assays against various cells. They showed positive reactions with transitional cell carcinoma of the bladder and negative with normal bladder. BIU-H6 did not react with any of the other normal or malignant tissues. The subclass of immunoglobulin for BIU-H4,6 was IgG1, for BIU-H9 is IgG2 alpha. The molecular weight of the antigen recognized by BIU-H6,9 is 102 KD.