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[Heterogeneity of the population of Escherichia coli thr- by assimilation of 3H-threonine].

The incorporation and spatial localisation of [3H]threonine in the cells of Escherichia coli RP 477 (leu-his-thr-B1-) were studied using the techniques of radiometry and adsorption electron-microscopic autoradiography with additional time-lapse microfilming to follow up the growth of the population. The strain was shown to be heterogeneous in its metabolic, reproductive and morphological (cell diameter) characteristics; a generative cluster was revealed. Indirect data indicated that the so-called "resting" cells were functionally active.