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Cell Biological Characteristics by Fluorescence Microscope and Its Application in Animal Models of Muscle and Tendon Injuries

: With the progress and development of medical science, fluorescence microscope has been widely used in biological research. Muscle and tendon injuries, as common sports injuries, bring a lot of inconvenience to people's daily life and work. At present, the commonly used methods of repairing muscle and tendon injury have certain curative effect, but the treatment cycle is long and the recovery degree is low. In order to better detect the biological characteristics of cells, design muscle and tendon injury model, and explore the treatment of muscle and tendon injury, fluorescence microscope was introduced into the experimental process. Firstly, the muscle satellite cells and tendon stem cells of mice were cultured and passaged. After cryopreservation and resuscitation, the biological characteristics of the cultured cells were detected. The proliferation and cloning of the cells were observed by fluorescence microscope, and the growth curve was drawn. Then the muscle injury model and tendon injury model were established by cardiotoxin and collagenase type I respectively. Paraffin section was made for observation and serum CK index was detected. The experimental results show that fluorescence microscope can effectively observe the biological characteristics of cells and help to establish muscle tendon injury model. It is concluded that muscle satellite cells and tendon stem cells can recover the injury. This provides experimental basis for the clinical application of cell therapy in the treatment of muscle tendon injury.