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In vitro regeneration of Vigna unguiculata using marine seaweed Sargassum polycystum

We have developed simple and efficient regeneration protocol using plant growth regulators and naturally available seaweed. Efficiency of Sargassum polycystum in promoting the growth of Vigna unguiculata at in vitro condition were studied. Equal sized seeds were inoculated in different concentration of seaweeds, we found that 20% of extract shown 100% germination compared to control. Initially, TDZ and Kin were tested for the multiple shoot induction. Best responded concentration was tested in combination with the seaweed extract Sargassum polycystum. We observed increase in the multiple shoot induction from 10.2 shoots per explant to 14.8 shoots per explant. Seaweed extract induced shoot elongation and rooting at in vitro condition. So we believe that, seaweed extracts has biostimulant activity, the activity of the seaweed extract is due to presence of more than one plant promoting substances.