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[Changes in various differentiation-related markers after differentiation induction of a pluripotent human embryonal carcinoma (EC) cell line by hexamethylene bisacetamide].

A pluripotent human EC cell line (NEC14) could be induced to morphologically differentiate by treatment with 10(-2) M HMBA for 3 days in vitro. The changes in various differentiation-related markers (cell surface antigens, lectin binding sites, intermediate filaments, secreted products and extracellular matrix proteins) after induction of differentiation were examined in order to clarify the differentiation lineage. The results were as follows: 1) The most conspicuous changes in cell surface antigens after differentiation were the expression of major human histocompatibility antigens (HLA-A,B,C) and the changes in stage specific embryonic antigens (SSEA-1-/SSEA-3(+)----SSEA-1+/SSEA-3-). 2) Vimentin, mesenchymal intermediate filament, was only detected after the differentiation. 3) Tenascin, an extracellular matrix protein produced in mesenchymal cells, was produced after the differentiation. These results indicate that HMBA can induce NEC14 cells to differentiate into mesenchymal elements of embryonal mesoderm.