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Rapid electrofocusing of erythrocyte acid phosphatase

A routine method is described for obtaining reproducible erythrocyte acid phosphatase patterns by ultrathin‐layer polyacrylamide gel isoelectric focusing. Electrofocusing of EAP on 0.2 mm thick gels took 30 and 22 min on the Ultrophor and Cold Focus Apparatus, respectively, when the distance separating the electrodes was 5.4 cm. This method, which reduces the cost for focusing EAP, can be ideal for many laboratories interested in EAP phenotyping.