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Study of some parameters affecting the in vitro cultivation of Plasmodium falciparum within Saïmiri sciureus red blood cells.

The in vitro growth and multiplication of the erythrocytic stages of Plasmodium falciparum within Saïmiri sciureus (squirrel monkey) red blood cells have been studied. Various parameters, such as the origin of the red blood cells and serum supplement, nature of the buffer, influence of the final pH of the medium, role of proteose peptone and glucose addition, were investigated. The selection of the best culture conditions led to the obtention of a reproducible in vitro growth of two parasite cycles in Saïmiri erythrocytes, which is an useful achievement for in vitro studies. Our failure to establish a continuous culture line for longer than 19 days, could be explained by a dramatic increasing of osmotic fragility of the Saïmiri red blood cells related to their small size.