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Effect of Enamel Pretreatment with Pastes Presenting Different Relative Dentin Abrasivity (RDA) Values on Orthodontic Bracket Bonding Efficacy of Microfilled Composite Resin: In Vitro Investigation and Randomized Clinical Trial

Bonding failure is a clinical issue frequently encountered in orthodontic practice. The aim of this study was to evaluate enamel pretreatment both in vitro and clinically using agents with different RDA values before brackets’ bonding, to assess if RDA can affect the Shear Bond Strength (SBS), Adhesive Remnant Index (ARI) and clinical failure rate of orthodontic brackets. For the in vitro study, 220 bovine teeth were pretreated with agents with different RDA values. Subsequently, brackets were bonded. For the clinical study, 20 patients underwent bonding of 20 brackets each with a split-mouth design. Low and high RDA toothpastes were used for enamel pretreatment. SBS, ARI and failures were recorded. Higher SBS values were found for teeth pretreated with lower RDA agents; conversely, lower SBS values were found for teeth pretreated with higher RDA agents (p < 0.05). For high ARI values, RDA increased too (p > 0.05). In the clinical study, a significantly lower failure rate was reported for teeth pretreated with low RDA toothpaste (2.5% in low RDA group, 7.0% in high RDA group; p < 0.05). No significant differences were assessed comparing the two dental arches and anterior and posterior sites. Enamel pretreatment with low RDA toothpastes could increase brackets’ survival rate. Further in vitro and clinical studies would be welcomed to confirm these findings.


Introduction
The aims of orthodontic therapy are the improvement of oral health conditions and, as a consequence, better facial aesthetics, which are the reasons of the increasing request of treatment by adults [1]. Nowadays, different treatments are available: fixed appliances, with labial or lingual brackets, and clear aligners [2,3]. Focusing on labial fixed appliances, the main discomforting situations consist of oral mucosal lesions, such as erosions, desquamations and ulcerations caused by brackets and archwires [4,5]. In addition, breakages of wires and brackets detachments can occur [5]; the problem is relevant as 61% of recalled patients from all the considered cohorts reported a breakage of a fixed orthodontic appliance [6].
During orthodontic therapy, a common but unpleasant occurrence is the possibility of brackets' detachment, which can cause the lengthening of treatment time and give discomfort to the patient [7][8][9][10][11]. A reasonable clinical failure rate should be below 10%,

Specimen Preparation
The Unit Internal Review Board (2018-0530) approved this study. For the in vitro study, 220 freshly extracted bovine lower incisors were collected. They had to meet the following criteria: vestibular and lingual enamel integrity, absence of traumatic lesions related to avulsion and absence of caries.
Established alpha = 0.05 and power = 90%, and considering the SBS primary outcome, sample size calculation required 220 total units. An expected value of 12.6 was hypothesized with a standard deviation of 1.5 [16]. The expected mean difference was supposed to be 2.2. Therefore, 10 samples were required for each group.
After the extraction, teeth were stored in a solution of thymol 0.1% (w/v) for a week, at 4 • C temperature [22,23]. Initially, periodontal ligament and gingival tissues was cleaned from each tooth with the help of a scalpel; then, they were embedded into cold-curing fast-setting acrylic (Leocryl, Leone s.p.a., Sesto Fiorentino, Italy) inside a plastic cylindrical mold (2 cm height × 2 cm diameter) [24].
Teeth were randomly divided into 22 groups of 10 elements each in order to be subjected to a 1-min pretreatment with different toothpastes and polishing compounds ( Table 1). They showed similar values of fluoride (from 1100 to 1450 ppm) so that this variable could not influence the outcome of the study. In the following section, the pretreating agents used are ordered according to growing values of Relative Dentin Abrasivity (RDA), starting from the commercially available product with the lowest RDA. Group 22-control: teeth from this group were not pretreated with toothpastes before orthodontic bonding, but they were just brushed with water for 1 min.
Groups 2, 7 and 8 were pretreated with the corresponding polishing paste with a toothbrush mounted on a low-speed handpiece for 1 min; successively, the enamel surface was rinsed for 3 s, with the aim of removing residues [25]. Group 4 was treated with a specific handpiece (KaVo Dental GmbH, Biberach an der Riß, Germany) for the use of glycine and bicarbonate; then, all teeth were rinsed for 30 s. Groups 1,3,5,6,9,10,11,12,13,14,15,16,17,18,19,20 and 21 were pretreated with toothpastes with different RDA values for 1 min, using a medium-bristled toothbrush for home oral care, to simulate manual cleansing before orthodontic bonding; then, all the incisors were rinsed for 20 s [25]. Group 22 was not treated and served as control.
A total of 220 0.022" SWM (Sweden & Martina S.p.A., Due Carrare, Padua, Italy) stainless steel brackets were applied on the vestibular surfaces of teeth following a common protocol for bonding [26]: the vestibular surface of the teeth was etched for 30 s with 37% orthophosphoric acid (Gerhò Etchant gel 37%, Gerhò spa, Terlano, Italy), and then it was rinsed and dried; a thin layer of Transbond XT Light Cure Adhesive Primer (3M Unitek, Monrovia, CA, USA) was applied and then cured for 10 s with a LED curing unit (Starlight Pro, Mectron s.p.a., Carasco, Italy); the bonding of the orthodontic brackets on teeth surfaces was performed with the application of Transbond XT Light Cure Adhesive Paste (3M Unitek, Monrovia, CA, USA) on their base; brackets were applied on the vestibular surfaces with a light pressure to allow the squeezing of the composite from the bracket base [27]. Then, they were correctly oriented and extra paste was removed with a probe; the adhesive paste was cured with the LED curing unit at a distance of 2 mm from the enamel-bracket interface, 10 s on the mesial surface and 10 s on the distal one. Finally, specimens were stored in a thymol solution 0.1% (w/v) at room temperature [28]. The characteristics of the materials used and the protocols recommended for their application are shown in Table 2.

Shear Bond Strength (SBS) Test
SBS was evaluated for each tooth using a universal testing machine (Model 3343, Instron, Canton, MA, USA) [29]. Specimens were positioned in the lower jaw of the machine, at the exact center of their mold and inserted in a way that the shear force is exerted parallel to the bases of the brackets. The load was exerted in an occlusal-gingival direction, and the blade of the machine was set at 1 mm/min speed [30]. The maximum load necessary for the detachment was registered in Newton, using the software Bluehill 2 (Instron Industrial Products, Grove City, PA, USA). Data were converted into megapascals, knowing the area of the bracket's base (MPa = N/mm 2 ).

Adhesive Remnant Index (ARI) Score
The bases of the brackets and the surfaces of bonding were analyzed at X 10 magnification with a microscope (Stereomicroscope SR, Zeiss, Oberkochen, Germany), in order to determine the remaining adhesive after the detachment; therefore, ARI [30,31] was calculated with the following scoring criteria: 0: no adhesive; 1: less than 50% of adhesive remaining; 2: more than 50% of adhesive remaining; and 3: 100% of adhesive left.

Randomized Clinical Trial (RCT) 2.2.1. Trial Design
The Unit Internal Review Board (2019-0403) approved the study. It was a parallel group, randomized, active controlled, split-mouth and single-center trial with a 1:1 allocation ratio.

Sample Size Calculation
Sample size calculation (alpha 0.05; power = 85%) for two independent study groups and a dichotomous primary endpoint was performed. Concerning the variable "failure rates", an expected value of 2.08% was hypothesized. The expected difference between the percentages was supposed to be 6.78% [17]; therefore, 200 brackets per group were requested. Considering 20 brackets bonded for each patient, with a split-mouth design, 20 patients were necessary for each group.
Interim analysis and stopping guidelines were not applicable.

Participants
For this study, patients were enrolled from the Unit of Orthodontics and Pediatric Dentistry, Section of Dentistry, Department of Clinical, Surgical, Diagnostic and Pediatric Sciences, University of Pavia, Pavia, Italy. Recruitment started in June 2019 and the study ended in March 2021. Informed consent was obtained for each participant; for underage patients, the consent was signed by their parents. The inclusion criteria were patients scheduled for brackets' placement at least for one dental arch; only permanent teeth were considered for bonding. The exclusion criteria were teeth with bands placed, teeth with restorations, teeth with prosthetic crown and teeth with direct tubes.

Intervention
The aim of the clinical study was to investigate the failure rate of orthodontic brackets after a pretreating procedure with two different toothpastes. The split-mouth design enabled the possibility of using the same patient for the administration of two different treatments.
Patients from group A received enamel pretreatment with a low RDA toothpaste (Advance White Paste Baking Soda and Peroxide, Church and White, Ewing, NY, USA; RDA = 42) in the maxillary left and mandibular right quadrants, while the remaining quadrants were pretreated with a high RDA toothpaste (Colgate 2 in 1 Tartar Control/Icy Blast Whitening, Colgate-Palmolive, New York, NY, USA; RDA = 200). In group B, the quadrants were inverted. The sides were allocated using random number tables.
In Table 3 are shown the compositions of the two toothpastes; since fluoride can influence the curing procedure and alter bonding efficacy, two toothpastes had been chosen with the same fluoride content. Successively, 400 0.022" 3M (3M Unitek, Monrovia, CA, USA) stainless steel brackets were bonded on the vestibular surfaces of teeth. These surfaces were etched for 30 s with 37% orthophosphoric acid (Gerhò Etchant gel 37%, Gerhò spa, Terlano, Italy), then rinsed and dried; a thin layer of Transbond XT Light Cure Adhesive Primer (3M Unitek, Monrovia, CA, USA) was applied and then cured for 10 s with a LED curing unit (Starlight Pro, Mectron s.p.a., Carasco, Italy); the bonding of the orthodontic brackets on teeth surfaces was performed with the application of Transbond XT Light Cure Adhesive Paste (3M Unitek, Monrovia, CA, USA) on their bases; then, brackets were applied on the vestibular surfaces with a light pressure and correctly oriented, and extra paste was removed with a probe; the adhesive paste was cured with the LED curing unit at a distance of 2 mm from the enamel-bracket interface, 10 s on the mesial surface and 10 s on the distal one.

Outcomes
Data collection was carried out during monthly visits in a time period between 1 month and 12 months from the day of brackets' placement. Each detachment was registered distinguishing the specific tooth interested and assessing the time period from the initial bonding procedure. The teeth with the detached brackets were not further included in the study. Participants were reminded to attend the appointments and to immediately inform the orthodontist if any detachment had happened. No variation to the outcome occurred after the beginning of the trial.

Randomization and Blinding
A randomization sequence was generated by the data analyst thanks to a block randomization table; a permuted block of 20 participants was considered. Participants were allocated by the operator, who enrolled them using sequentially numbered and sealed envelopes with the allocation cards previously prepared. The operator, participants, data assessor and data analyst were always blinded during the study and none of them knew which treatment toothpaste had been used. Patients could not note any differences except from the two tastes of the toothpastes.

Allocation Concealment
The operator who enrolled participants also achieved the allocation concealment using sequentially numbered and sealed envelopes containing allocation cards that had previously prepared. The randomization list generated was held securely in remote location.

Statistical Methods
Data analysis was conducted with R software (R version 3.1.3, R Development Core Team, R Foundation for Statistical Computing, Wien, Austria). For SBS values, descriptive statistics were calculated for each of the twenty-two groups. Data included mean, standard deviation, minimum, median and maximum SBS values. The Kolmogorov-Smirnov test was used for calculating data normality. Successively, an ANOVA test was performed, followed by Tukey's test for post-hoc analysis. A Pearson correlation coefficient was calculated to determine whether there was any correlation between SBS and RDA values.
For the ARI score, a χ 2 test was conducted to assess significant differences between the twenty-two groups. A Spearman correlation coefficient was calculated to determine any correlation between ARI and RDA values.
The aim of the RCT was assessed with a Fisher exact test to determine differences among the frequencies of brackets' detachments of the two experimental groups; finally, Kaplan-Meier survival curves of group 1 and 2 were constructed and compared using the log-rank test.
Significance for all statistical tests was predetermined at p < 0.05.

Shear Bond Strength (SBS) Test
Descriptive statistics for the twenty-two groups are shown in Table 4 and Figure 1. The ANOVA test showed significant differences between groups (p < 0.0001

Adhesive Remnant Index (ARI)
Descriptive statistics for the twenty-two groups are shown in Table 5. The χ 2 test showed that there is a statistically significant higher frequency of ARI scores for 2 and 3 of the twenty-two groups (p < 0.05). Spearman coefficient showed a weak correlation between ARI and RDA values (r = 0.2467).

Adhesive Remnant Index (ARI)
Descriptive statistics for the twenty-two groups are shown in Table 5. The χ 2 test showed that there is a statistically significant higher frequency of ARI scores for 2 and 3 of the twenty-two groups (p < 0.05). Spearman coefficient showed a weak correlation between ARI and RDA values (r = 0.2467).

Randomized Clinical Trial (RCT)
A total of 20 patients (13 females, mean age 17 years and 8 months; 7 males, mean age 17 years and 6 months) were enrolled for the study as they agreed with the inclusion criteria. They all received allocated intervention and none of them were excluded from analysis. The flow chart of the study is shown in Figure 2.

Randomized Clinical Trial (RCT)
A total of 20 patients (13 females, mean age 17 years and 8 months; 7 males, mean age 17 years and 6 months) were enrolled for the study as they agreed with the inclusion criteria. They all received allocated intervention and none of them were excluded from analysis. The flow chart of the study is shown in Figure 2.  As shown in Table 6, statistically significant differences in the cumulative failure rate were found between the two groups (p < 0.05). No statistically significant difference was found between anterior and posterior sites ( Table 7) and between the two dental arches (Table 8).  Figure 3. During the time frame of the study, a statistically significant difference was found between the two groups (hazard ratio: 0.34; C.I. 95%: 0.15-0.92; log rank test: p = 0.0315). No statistically significant difference was found between anterior and posterior sites ( Table 7) and between the two dental arches (Table 8).  Figure 3. During the time frame of the study, a statistically significant difference was found between the two groups (hazard ratio: 0.34; C.I. 95%: 0.15-0.92; log rank test: p = 0.0315).

Discussion
Brackets' detachment is a widely investigated issue, with an extensive literature of in vitro and clinical studies [8,10,[12][13][14]23]. The aim of the present study was to test different pretreating agents to find eventual differences among the products in order to assess which could cause lower detachment rate.
The first null hypothesis of the present report has been rejected because there were significant differences among the groups as regards SBS values. The main variable considered is have SBS and higher SBS values been found among in vitro studies [32] due to the ideal conditions in which they are conducted; surely, the absence of a wet environment is a significant contributing factor in the adhesion process [21]. However, in vitro experimentations are useful to predict the behavior of materials tested when used in the oral environment.
The rationale of the present study, which involved both in vitro and clinical phases, was to assess if there could be a role of enamel pretreatment in the failure rates of brackets bonding, when toothpastes with different RDA values are used before the acid etching procedure.
The use of bovine teeth is a limitation of this study. However, despite shape and size differences, they show similar physical properties in regard to human teeth, therefore, they can be used instead of them for in vitro experimentations [41].
The in vitro part of the present study showed that higher RDA values correspond to significantly lower SBS values, and this trend was confirmed by the Pearson coefficient, which showed a moderate negative correlation between SBS and RDA values (r = −0.5779). This means that only 33.3% of the variance of SBS is explained by the RDA. Due to the fact that no previous similar studies have been conducted, it is not possible to compare the Pearson correlation coefficients.
The control group showed SBS values similar to those of other in vitro studies that used Transbond XT as the adhesive system with a similar protocol [15,34,37]; Huilcapi and colleagues [9], instead, used a different adhesive system, but the results are still comparable.
The study of Cossellu and colleagues [15] is the only one that used glycine and a toothpaste in enamel pretreatment, as in the present work. Additionally, also fluoride varnish, casein-phosphopeptide-amorphous calcium-phosphate (CPP_ACP), ozone and hydroxyapatite powder were tested. These three substances did not compromise on bracket bond strength. Conversely, fluoride, glycine and hydroxyapatite significantly decreased the SBS. Anyway, only the fluoride group showed significant clinically low (<6 MPa) SBS values.
In detail, Air-Flow Perio containing glycine was used with the same protocol as for group 4 of the present study, together with the adhesive technique used; SBS values are comparable (11.02 MPa vs. 12.44 MPa, respectively). The toothpaste used is Biorepair Plus (RDA~70), which has similar RDA to Colgate Total (RDA = 70) toothpaste used in group 11 (16.01 MPa vs. 11.63 MPa, respectively). However, it should be highlighted that the latter contains fluoride (1450 ppm), while the former does not. The control groups show a slight difference between SBS values (17.38 MPa vs. 11.01 MPa).
The study of Mahajan and colleagues [39], instead, used flour of pumice similarly to the present work for group 8, but its RDA value is unknown, and a Self-Etch Primer was used for brackets' bonding, therefore, a proper comparison is not suitable.
Enamel pretreatment before acid etching could improve adhesion values, because its purpose is to remove the biofilm present on the surface of teeth and allow a better adhesion of the bracket [40]; however, the strength of adhesion to the teeth is highly influenced by its degree of demineralization [26]. According to a recent systematic review, the incidence of white spot lesions in orthodontic patients is widely variable [42] and different solutions have been proposed, concluding that a fluoride toothpaste is the best treatment option in absence of evidence-based findings. The results of fluoride application before, during and after the acid-etching procedure are controversial [43]; nevertheless, the in vitro study tested pretreating agents with almost the same quantity of fluoride, with the aim of limiting its effects on the bonding procedures.
All SBS values are included within 5-50 MPa, which is considered a clinically acceptable theoretic range for orthodontic biomaterials [30].
The second null hypothesis of the present study was rejected because there were significant differences among the groups as regards ARI scores. In fact, there was a statistically significant higher frequency of ARI scores for 2 and 3 in the 22 groups of the in vitro study. The Spearman correlation coefficient showed a weak correlation between ARI and RDA values. According to a previous report in which powders and toothpastes were compared [15], lower scores of ARI have been found for both the pretreatment agents, while in the present work, higher ARI scores were found. The same scenario occurred for the control groups. Moreover, it was not possible to consider a comparison with the work of Mahajan and colleagues for the flour of pumice [39] because ARI evaluation was not performed.
ARI is one of the most used methods for the evaluation of adhesive systems for the bonding of orthodontic brackets [44]. A score of 0 is related to low SBS values and to contaminants over enamel; a score of 3, instead, means less risk of enamel fracture after debonding, but the removal of the remnant adhesive on enamel surfaces is longer and should be done carefully [30].
Finally, also the third null hypothesis was rejected. When enamel was pretreated with low RDA toothpaste, lower failure rates were reported.
Toothpastes are widely used for routine home oral care [45], and evaluation of their values of abrasion with reference to enamel and dentin (Relative Dentin Abrasion-RDAand Relative Enamel Abrasion-REA-values) should be taken into consideration in the choice of a toothpaste, considering that they could play a role as pretreating agents. However, not all toothpastes show REA values [19], therefore, RDA is one of the parameters that deserves to be investigated as a potential modifier of the traditional bonding procedure of orthodontic brackets.
Various clinical studies tried to estimate the failure rate of orthodontic brackets, comparing brackets of different materials [8], types of orthodontic bonding [23], adhesion procedures [46] and adhesive materials [7,17,47]. In addition, retrospective studies investigated the issue, trying to assess correlations between brackets' failure rate and multiple variables [11,12,48].
To date, there is no clinical study that has evaluated the efficacy of toothpastes as pretreating agents. Generally, the most used pretreatment agent is pumice together with water, with a rubber cup mounted on a low-speed handpiece. The study of Burgess and colleagues [49] evaluated the effect of pumice and water as enamel pretreating agents on brackets' adhesion, using a rubber cup, reporting a significant difference between pumiced and non-pumiced groups, as brackets placed on pumiced teeth showed lower failure rates. Other clinical studies considered enamel pretreatment with a rubber cup, pumice and water for metallic brackets bonding.
Krishnan et al. [47] adopted the same adhesion protocol and materials as in the present study, showing a failure rate of 8.1%. For the other group, in which a flowable resin composite was used (Heliosit Orthodontic, Ivoclar Vivadent AG, Schaan, Liechtenstein), a lower failure rate (6%) was reported. The study of Dominguez et al. [46] compared two different adhesion protocols: 37% etching + Transbond XT paste and Transbond Self Etch Primer + Transbond XT paste (3M Unitek, Monrovia, CA, USA) with previous pumicing with a rubber cup. The survival rates for the two groups were, respectively, 5.41% and 4.58%.
Ogiński et al. [8] found a 7.2% failure rate for 100 stainless steel brackets in a twelvemonth study, but even though the same adhesive material was used (Transbond SEP + XT, 3M Unitek, Monrovia, CA, USA), neither pretreatment nor etching with 37% orthophosphoric acid were executed. Kafle et al. [12] found a failure rate of 3.34% for metallic brackets, but information about materials and protocol used are missing.
Among all these pretreating agents used, further analyses are required to understand what happens to pretreated enamel. As an example, Ravichandran et al. [49] used optical coherence tomography to assess in vitro enamel loss after pumicing and etching. The findings of this work highlight that the combination of the two procedures increases enamel loss with a slight difference between etching only. However, it should be evaluated if possible residual remnants of pumice lie on the enamel surface, thus hindering the adhesion procedure. Moreover, other evaluations should be performed, with other pretreating agents, to assess the changes on the enamel surface after the procedure.
The lack of standardized procedures for the bonding of orthodontics brackets in the abovementioned clinical studies leads to a variety of different materials used and protocols adopted. For this reason, a direct comparison among the failure rates of brackets bonded on pretreated enamel is not possible. However, an analysis of the failure rates could help in determining the possible role of pretreatment procedures in the adhesion process.
The failure rates of the previous studies, including the present research, are within the range of clinical acceptability of 10% [12], except from the study of Burgess et al. [50]. In detail, the failure rate of the group pretreated with the low RDA toothpaste (2.5%) is the lower value among the abovementioned ones, although different protocols were used; the survival rate of the trial group with high RDA toothpaste pretreatment is similar to other previous reports (7%). Since the RDA values of pumice flours used is unknown, together with the time of execution, a clear role of the pretreatment procedure is uncertain. However, in this study, a statistically significant difference has been found between the two groups. Other variables should be considered in the assessment of failure rates causes. For example, Sukhia et al. [48] developed a multivariate estimated model with different factors that could affect the bonding failure rate, including bracket material, jaw (maxilla/mandible), overjet, overbite, site (anterior/posterior), side (left/right) and the interaction between site and side. The results highlighted that in the posterior region the risk is considerably higher if compared to the anterior region; also, mandibular brackets were more prone to fail if compared to the maxillary ones.
In particular, the risk of bracket failure on the right posterior region appears to be 7.7 times that in the right anterior region when adjusted for all other variables in the model (HR: 7.7; 95% CI: 4.3-13.6).
Stasinopoulos et al. [11] considered linear bi-and multivariate regression; they concluded that posterior brackets were more prone to failure. Specifically, considerable differences existed according to tooth type, with an 8.0% failure of canines, followed by 11.3% for first premolars, 13.1% for central incisors, 14.4% for lateral incisors and 23.4% for second premolars.
These data are partially in agreement with the present report, as failure rates were higher in the lower and posterior sites but with no significant difference. Additionally, the results of the present report agree with another study that tested orthodontic splints using the same polymeric composite resin and that reported no significant difference between upper and lower frameworks [28].
Due to the absence of previous similar randomized clinical trials on pretreating agents in orthodontics, further studies are desired to compare the results obtained here. Additionally, one of the limitations of the present study is that the toothpastes are not directly comparable because they differ not only in terms of their RDA but also their composition. In addition to that, the fluoride level cannot be completely controlled because manufacturers commercialize their products with inconstant fluoride percentages. Another concern in the clinical study is that the malocclusion type was not taken into consideration. Therefore, future studies testing other variables and with longer follow-up are expected to clarify the best enamel pretreatment protocol before bracket bonding. Moreover, it could be hypothesized that the increase of the amount of debris left on the enamel after the pretreatment procedure, along with its increasing roughness, are responsible for the low SBS values found in the case of using high RDA agents [51]. Since it was not the purpose of this study, it would be interesting to perform additional microscopical and chemical evaluations, respectively with SEM (Scanning Electron Microscopy) and EDS (Energy Dispersive X-ray Spectroscopy) analysis, in order to better understand how pretreating agents affect enamel's morphology and composition. Moreover, the different bristles' hardness of the rubber cup used for the pretreatment procedure might have an influence on the parameters tested, which should be assessed. In fact, despite RDA being a standardized and reasonably valid tool to determine the abrasive power of toothpastes, behavioral differences among individuals significantly influence the potential of abrasion of a specific agent, independently of its RDA value [52].
Due to the high number of commercial materials tested in this study, further variables, like the presence of different excipients in the products, should be taken into account. Finally, other pretreatment methods (like airflow) deserve to be tested and compared with respect to the treatments considered in the present study.

Conclusions
Enamel pretreatment before standard adhesion protocols for orthodontic brackets with low RDA toothpastes could be more suitable than those with higher RDA values as their bonding survival rate is significantly higher. Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.
Data Availability Statement: Data are available upon reasonable request to the Corresponding Authors.

Conflicts of Interest:
The authors declare no conflict of interest.