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[Determination of protocatechuic acid in Blumea riparia (Bl.) DC. by RP-HPLC].

OBJECTIVE
To determine the content of protocatechuic acid in Blumea riparia by RP-HPLC.


METHOD
mu-Bondapak C18 column was used, mobile phase consisted of methanol-water-glacial acetic acid(19:80:1) and detection was performed at UV 260 nm.


RESULT
The standard curve was linear in the range of 3.31-41.8 micrograms.ml-1. The correlation coefficient was 0.9999. The average recovery rate and RSD were 98.05% and 1.94% (n = 6) respectively.


CONCLUSION
The method provides scientific indexes for quality control of riparia.