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Chemiluminescence Flow-Through Sensor for Determination of Reserpine in Pharmaceutical Preparation and Biological Fluids Using Immobilized Reagents Technology

Abstract A sensitive continuous-flow sensor based on chemiluminescence (CL) detection was developed for the determination of reserpine at nanogram level by the immobilization of the reagents. It was found that the CL intensity from the oxidation between luminol and periodate could be decreased in the presence of reserpine. The decrease of CL emission was correlated with the reserpine concentration in the range from 1.0 to 300 ng mL−1, and the detection limit was 0.3 ng mL−1 (3σ). The system could produce an evident CL signal by water as eluent and it was also shown that the flow sensor could greatly improve the selectivity and sensitivity for determination of reserpine with a high signal-to-noise ratio. A complete analysis, including sampling and washing, could be performed in 0.5 min with a relative standard deviation of less than 3.0%. The proposed procedure was applied successfully in the assay of reserpine in pharmaceutical preparations and biological fluids samples. The flow sensor offered reagentless procedures and remarkable stability in determination of reserpine, and could be easily reused over 80 h.