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Detection of peritoneal micrometastasis by reverse transcriptase‐polymerase chain reaction for heparanase mRNA and cytology in peritoneal wash samples

Peritoneal dissemination is the most common pattern of metastasis in advanced gastric carcinoma with serosal invasion. In the present study, we have reported the clinical relevance of a new diagnostic method with reverse transcriptase‐polymerase chain reaction (RT‐PCR) assay using heparanase as a target gene for detection of free cancer cells in peritoneal washes.