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Morphology Preserving Segmentation Method for Occluded Cell Nuclei from Medical Microscopy Image

Nowadays, image segmentation techniques are being used in many medical applications such as tissue culture monitoring, cell counting, automatic measurement of organs, etc., for assisting doctors. However, high-level segmentation results cannot be obtained without manual annotation or prior knowledge for high variability, noise and other imaging artifacts in medical images. Furthermore, unstable and continuously changing characteristics of all human cells, tissues and organs manipulate training-based segmentation methods. Detecting appropriate contour of a region of interest and single cells from overlapping condition are extremely challenging. In this paper, we aim for a model that can detect biological structure (e.g. cell nuclei and lung contour) with their proper morphology even in overlapping or occluded condition without manual annotation or prior knowledge. We have introduced a new optimal approach for automatic medical image region segmentation. The method first clearly focuses the boundaries of all object regions in a microscopy image. Then it detects the areas by following their contours. Our model is capable of detecting and segmenting object regions from medial image using less computation effort. Our experimental results prove that our model provides better detection on several datasets of different types of medical data and ensures more than 98% segmentation rate in the case of densely connected regions.