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FLUOROPHORES IN POLAR MEDIA: SPECTRAL EFFECTS OF THE LANGEVIN DISTRIBUTION OF ELECTROSTATIC INTERACTIONS

The models employed for the analysis of the dependence of the fluorescence spectrum upon the dielectric properties of the solvent'-5 consider the latter as a continuum which causes changes in the electronic energy levels through the interaction of the fluorophore with the reaction field that it induces upon its dielectric environment. In models of this kind, the change in energy of the excited state with respect to an unperturbed state is given by an expression of the form: