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Biological Response of Chondrocytes to Hydrogels

Abstract: Primary bovine chondrocytes were encapsulated in alginate and alginate combined with cartilage matrix extract, Cartrigel, for the purpose of cartilage tissue engineering. The cell constructs were incubated in vitro and gene expression of cartilage‐specific extracellular matrix molecules was quantitated and localized with in situ hybridization with a decrease in expression observed in the alginate‐Cartrigel constructs. Further understanding of cell response to scaffolds will allow rational design and development of hydrogels for cartilage tissue engineering.