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Effect of Conditioned and Co Culture Media from The Umbilical Cord Mesenchymal Stem Cells on Heptoma Cell Line (Huh7)

Background: this study aimed to detect a new way for cancer control. Hepatoma cell line HuH7 was the model for cancer and was treated with two types of the media. The first was the conditioned media (in which mesenchymal stem cells were cultured), the second media was co-culture media in which mesenchymal stem cells and HuH7 cells were cultured. Objective: this work was designed to evaluate the efficiency of using the media in which mesenchymal stem cells alone (Conditioned media) or co-culture with HuH7 (Co-cultured media) were housed on controlling the viability, growth and mortality of HuH7, by applying different techniques. Result: at specified times (0, 48,72,96 hours) after incubation in each medium cells were tested. The morphological study showed that in both conditioned and co-culture media, HuH7 cells were suffered from damage and this was directly proportional with increase of time. (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) tetrazolium reduction assay (MTT) showed that there was a significant difference between the four groups in conditioned and co-culture media. In the biochemical study for conditioned and co-culture media, there was a significant difference between the four groups in αFP and GOT, but there was a non-significant difference between the four groups in albumin, GPT, ALP and γ –GT. Conclusion: the conditioned and co-culture media from mesenchymal stem cells alone or co-culture with HuH7 proved to be efficient in control of cancer cell survival.