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Automatic analysis of G-banded human chromosomes by fast scanning microscope photometry. 2. Practical applications.

The practical applications and improvements to a fast scanning microscope photometer system are described. The production of a density profile is demonstrated which, when taken in conjunction with the chromosome scan, allows more information to be extracted from the data than was previously possible. This enables a more accurate correlation to be made between phenotype and karyotype. The system is demonstrated on preparations of known cytogenetically abnormal individuals, showing the range of problems which can be tackled.