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Folic acid and cyanocobalamin effects on the expression of genes involved in DNA methylation of the oyster
 Crassostrea gigas

The microalga Chaetoceros calcitrans was cultured with three concentrations of vitamin B12 (cyanocobalamin) to supplement the feed for the oyster Crassostrea gigas. In two additional assays, vitamins B12 and B9 (folic acid) were dissolved in the water to increase the methyl donors necessary for the one‐carbon cycle and DNA methylation. Microalgae did not present differences in their concentration of vitamins, and no correlation was found between the concentration of vitamin supplement and the vitamin concentration in oysters; however, changes in the expression of genes involved in the DNA methylation were found. The expression of proton‐coupled folate transporter (PCFT) was lower in oysters supplemented with Vit. B9. The expression of proton‐coupled folate transporter (PCFT) was lower in oysters supplemented with Vit. B9., however, the expression of genes implicated in the release of the methyl used to maintain methylation such as methionine synthase (MS) and DNMT1 expression was high, suggesting faster transportation of Vit. B9. The methylenetetrahydrofolate reductase (MTHFR), implicated in the reduction of folic acid, was lower in oysters supplemented with Vit. 9 and B12 dissolved in the water. DNA methyltransferase DNMT1A, involved in DNA maintenance, was higher in oyster supplemented with Vit. B9, but the expression of DNMT1B was not different. In conclusion, the supplementation with folic acid increases the expression of genes implicated in the maintenance of DNA methylation in C. gigas.