{'fraction_non_alphanumeric': 0.027266530334014997, 'fraction_numerical': 0.006816632583503749, 'mean_word_length': 6.68586387434555, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 1, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Identification of mitochondrial and non‐mitochondrial glutaminase within select neurons and glia of rat forebrain by electron microscopic immunocytochemistry

Antibodies against the mitochondrial enzyme glutaminase (EC 3.5.1.2), have been used in previous immunocytochemical studies to help identify glutamatereleasing neurons among all glutamate‐containing neurons. The studies were based on the idea that glutaminase is enriched within the releasable “transmitter” pools of glutamate. However, evidence is also available to suggest that the enzyme does not occur exclusively within glutamate‐releasing neurons. Thus we sought to determine whether glutaminase was immunocytochemically detectable within presynaptic terminals forming asymmetric (putatively excitatory) synapses or, alternatively, occurs in association with mitochondria throughout the cell. For this purpose, we examined the cellular and subcellular distribution of glutaminase‐ immunoreactivity in neocortical (vi‐sual and somatosensory) areas known to contain glutamatergicperikarya. This localization was compared with the distribution in striatal(caudateputamen and nucleus accumbens) regions recognized to contain high densities of glutamatergic terminals out fewer, if any, glutamatergic perikarya. Glutaminase‐immunoreactive perikarya were numerous within the infragranular, lamine of neocortex (∼1 per 1,000 μm2 tissue area) but sparse within me caudate‐putamen nuclei and accumbens nuclei, (