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Periodic Paralysis: A Case Report of Normokalemic Presentation in a Young Girl

The skeletal muscle channelopathies represent a rare group of neuromuscular disorders which are caused by genetic mutations regarding voltage-gated ion channels, which play an important role in muscle membrane depolarization. Muscle channelopathies are broadly divided into 2 main categories: nondystrophic myotonias (NDM) and periodic paralysis (PP). Periodic paralysis (PP) are rare autosomal dominant neuromuscular disorders, characterized clinically by periodic attacks of muscle weakness in concomitance with serum potassium level alterations. It is possible to distinguish normokalemic, hyperkalemic and hypokalemic paralysis. PP is caused by genetic mutations in voltage-gate ion channels like sodium, potassium, and calcium channels, which are determinant for muscle membrane depolarization. The most common genes involved in pathogenesis are CACN1S, SCN4A and KCNJ2, encoding calcium, sodium, and potassium channels (Table 1). Moreover, paralysis related to serum potassium values may also occur in thyreotoxicosis [1], Liddle syndrome, Gitelman syndrome, primary hyperaldosteronism, and acid-base balance disorders. In this case report, we describe a case of transient paralysis and muscular weakness of both upper and lower limbs after a high carbohydrate meal on the day before clinical presentation. Potassium level was normal at the hospital admission, while high levels of creatine phosphokinase, (CPK), Myoglobin, and Aspartate Aminotransferases (AST) was observed. A missense mutation in CACNA1S exon 11 was identified. This case supports the importance of a correct family history, muscular enzyme analysis and genetic study in a normokalemic presentation of periodic paralysis.

Hypokalemic periodic paralysis is a rare disorder with a prevalence of 1:100.000 [1] and a large spectrum of penetrance which is expressed in a wide variety of correlation between genotype and phenotype [10]. Moreover, the quality of life in these patients can be impaired for a chronic and progressive muscular dystrophy [11,12]. Periodic paralysis is usually classified on clinical and laboratory criteria (serum potassium values and response to potassium administration) and on genetic criteria. Reliyng on serum potassium levels, periodic paralysis is classified in mormokalemic (NormoPP), hyperkalemic (HyperPP) and hypokalemic (HypoPP) [1,2]. HyperPP, NormoPP and 10% cases of HypoPP are related to SCN4A mutations7, 70% cases of HypoPP are related to CACN1AS mutations [3,4], on the other hand KCNJ2 mutations are decisive for Andersen-Tawill Syndrome [9,12]. Table 1: Main channelopathies and loci involved. In channelopathies mutations interested calcium, sodium and potassium channels. The gene loci involved are CACN1AS (calcium channel) located on chromosome 1, SCN4A and KCNJ2 (sodium and potassium channels, respectively) located on chromosome 17. The table describes different clinical presentations related to specific gene loci mutations [28,29].
Attacks are characterized by flaccid paralysis, usually occurring on awakening during the night or at early-morning, focal or generalized weakness which lasts for hours (occasionally days) with a gradual resolution [1,2,14,15]. Occurrence may be spontaneous or provoked by prolonged rest after vigorous exercise, carbohydrate-rich meal on the previous day. Triggers include viral infections, sleep deprivation, period in women and drugs (e.g. beta agonists, corticosteroids and insulin) [1,16]. HyperPP differs from HypoPP for an earlier age of onset (first decade) [1,16]. Weakness is usually generalized and last 1-4 hours (infrequently days). They are triggered by rest after exercise, K-rich foods, stress, and fatigue.
Among episodes of weakness, eyelid myotonia may be the only clinical sign. Electrical myotonia is found in 50-75% of patients.
Typically, hyperkalemic attacks are associated with elevated ictal serum potassium level, but many children have normal potassium level during attacks. For those reason, laboratory approach is important to distinguish them [1,16,17].
In our patient a transient paralysis and muscular weakness with elevated levels of creatine phosphokinase, (CPK), Myoglobin, and Aspartate Aminotransferases (AST), concurrently with normal potassium serum levels was observed.

Case Presentation
A 12-years old Caucasian girl was admitted to our clinic unit with acute onset of transient paralysis of all four limbs arisen on the awakening and the followed by paresthesia's, motor impairment without loss or disturbances of consciousness [1,16]. There was also muscular pain in both legs and arms without dysphagia, dysphonia, history of suggestive symptoms such as ocular, sensory, cerebellar, or cranial nerve involvement. Moreover, these symptoms improved after salt supplement intake. Family history was suggestive for muscle symptoms (not confirmed Liddle syndrome) [18]. Her grandfather, father and one of her uncles had similar mild paralytic attacks; on the other hand, another uncle presented more severe symptoms, including dyspnea and respiratory muscles involvement. She had a significant past medical history for transient paralysis.
One episode occurred after an acute diarrhea and two of them during physical exercise with an improvement after few hours.
On examination, skin was not affected by cutaneous alterations or lesions. Cardiovascular, pulmonary, and abdominal examinations were not compromise [19]. Neurological exam revealed a normal deep tendon reflexes and global and segmental strength of both upper and lower extremities [1,16,20]. There were not palpable goiter or tremors.
Laboratory findings of our case are shown in (Table 2). No ECG alterations was recorded [19,20]. Arterial pressure levels control were normal as well as the electroencephalogram. During hospitalization, the girl never showed neurological or muscular symptoms, so we prescribed oral potassium supplement only in case of need [21]. We planned a mutation screening for hypokalemic periodic paralysis. Genomic DNA was extracted from the peripheral blood and also other family members are tested with PCR amplification and direct sequencing of CACNA1S exon 11, the most frequent gene involved in hypokalemic periodic paralysis.
This mutation has been found in our patient and also in her father, in two uncles, grandfather and an asymptomatic cousin (Figure 1).  causes for the paralytic event [1,16]. A main cause of hypokalemia is acid-base disorder, so we tested blood gas analysis which showed compensated acidosis. This finding, associated to normal blood pressure values, has led us to exclude the diagnostic hypothesis of Liddle syndrome, postulated in the past for relative [22]. Liddle syndrome is one of the causes of muscular weakness, especially in lower limbs, associated with hypokalemia that occurs with metabolic alkalosis, hypertension, and hypokalemia. Same clinical manifestations are present in primary hyperaldosteronism, but aldosterone serum value in our patent was normal. Having regard to the symptoms' regression with the assumption of salt supplements, we concluded that it was a paralytic form due to hypokalemia [1,16,22]. For this reason, we investigated the major forms of hypokalemia. Besides Liddle syndrome and primary hyperaldosteronism, hypokalemia can be found in Gitelman syndrome associated with metabolic alkalosis, hypomagnesemia and hypocalciuria. In our patient, we did not find any alteration in urinary and blood electrolytes levels [23][24][25][26][27][28][29][30][31]. Thyrotoxicosis is another one of the main causes of secondary periodic hypokalemic paralysis [32]. Alhasan, et al. [13,33]. Excluding secondary causes and considering familiar paralytic episodes, genetic analysis in our patient and in her family was performed [23,24]. Genomic DNA was extracted from patient's peripheral blood and from members of her family using PCR amplification and direct sequencing of CACNA1S exon 11 [5,33].Finally, periodic paralysis in women is mostly asymptomatic (about 50% of cases) and normokalemic presentation is rarer than other clinical presentations. Therefore, it is important to have a clinical suspicion of periodic paralysis, after other causes of muscle weakness have been ruled out [18].

Consent
Written informed consent was obtained from parents of the patient for the publication of this case report. A copy of the written consent is available for being reviewed by the Editor-in-Chief of this journal.