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Replication of IPN Virus: A Cytochemical and Biochemical Study in SWT Cells

Summary Although IPN virus failed to multiply at 30°, it replicated at 16° and 22° in SWT cells. At 22° the viral eclipse period lasted nearly 6 hr with maximal virion titers attained by 24 hr, whereas replication at 16° was much slower. The replication of the virion was inhibited by 0.05 μg/ml of AD which did not interfere with the production of reovirus. Biochemical studies revealed that cellular DNA synthesis was markedly reduced (>50%) soon after infection, whereas total RNA synthesis was enhanced. The period of rapid increase in RNA synthesis paralleled the exponential production of infectious virus. Viral inclusion bodies, revealed by acridine orange-staining of virus-infected cells (SWT and RGG-2) late in the infectious cycle, were found to contain single-stranded RNA on the basis of their staining characteristics and sensitivity to RNase. inhibitory effect of higher temperature (33°) on IPN virus replication in FHM cells has been described (23). A plausible explanation for this phenomenon is that the viral mediated enzymes necessary for IPN virus replication possess optimal temperature requirements which differ from those of either of these cell systems. The maximum levels of RNA synthesis in IPN-infected SWT cells (6-8 hr p.i.) at 22°, 693