{'fraction_non_alphanumeric': 0.02899628252788104, 'fraction_numerical': 0.01858736059479554, 'mean_word_length': 5.289719626168225, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Promotion of Osteoblast Proliferation Activated by Betaglucan (BG) Derived from Yeast Sludge

In tissue engineering, biomaterials used for bone tissue substitutes attract increasing interests, especially for finding biologically active compounds that can activate proliferation of osteoblastic MG63 cells. The evaluation of the impact of a soluble yeast-derived β-(1-3), (1-6)-D-glucan (BG) extracted from distillery waste yeast sludge on viability and proliferation of MG63 cells was studied. Spray dried BG prepared from alkaline extraction was used as supplementary activator in osteoblastic cell culture system. The composition of BG was characterized using FTIR spectral analysis and BG analysis assay kit. MG63 human osteoblast cell-line was cultured on Dulbecco’s modified’s medium supplemented with various concentrations of BG ranging from 0.1 to 1.0 mg/mL. The cells were cultured up to 7 days under a humidified 5% CO2 atmosphere at 37°C and monitored the level of proliferation at pre-determined intervals. Results showed that increase in BG concentration substantially promoted MG63 cell proliferation. Optimal concentration was identified and found at 0.3 - 0.7 mg/mL. Results revealed that BG could be further utilized for the upregulation of osteoblastic proliferation positively related to the acceleration of bone regeneration.