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Flow-Calorimetric Biosensing Of Zinc(II) Ions With Use Of Thermolysin-immobilized Beads

Calorimetric biosensing of zinc(II) ions with use of thermolysin immobilized onto porous glass beads was tested. The sensing system was assembled with the beads-packed column mounted in a flow-calorimetric device for monitoring the enzyme activity. Temperature change at the outlet of the column was continuously detected. The metal-free enzyme(apoenzyme) was regenerated by exposing the column to EDTA solution(pH 4.0). Zinc(II) ions in submillimolar levels were determined through the activation of the apoenzymes.