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Determination of Acidic Metabolites of Biogenic Amines in Human Aqueous Humour by Gas Chromatography‐Negative Ion Chemical Ionisation Mass Spectrometry

Abstract: The concentrations of acidic metabolites derived from the biogenic amines o‐, m‐, and p‐tyramines (o‐, m‐, and p‐hydroxyphenylacetic acids), p‐octopamine/p‐synephrine (p‐hydroxymandelic acid), and dopamine (homovanillic acid and 3,4‐dihydroxyphenylacetic acid) were measured in human aqueous humour obtained from patients undergoing elective surgery for cataract removal or for trabeculectomy as a treatment for chronic open‐angle glaucoma. There were no clear differences in the pattern of metabolism of neurotransmitters between the two groups. An unexpected finding was that the o‐tyramine metabolite, o‐hydroxyphenylacetic acid, was present in aqueous humour.