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Evaluating color performance of whole-slide imaging devices by multispectral-imaging of biological tissues

The color reproducibility of two whole-slide imaging (WSI) devices was evaluated with biological tissue slides. Three tissue slides (human colon, skin, and kidney) were used to test a modern and a legacy WSI devices. The color truth of the tissue slides was obtained using a multispectral imaging system. The output WSI images were compared with the color truth to calculate the color difference for each pixel. A psychophysical experiment was also conducted to measure the perceptual color reproducibility (PCR) of the same slides with four subjects. The experiment results show that the mean color differences of the modern, legacy, and monochrome WSI devices are 10.94±4.19, 22.35±8.99, and 42.74±2.96 ▵E00, while their mean PCRs are 70.35±7.64%, 23.06±14.68%, and 0.91±1.01%, respectively.