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Screening of Various Raw Starches on Production of Thermostable Amylopullulanase by Clostridium thermosulfurogenes SVM17

3 Abstract: A thermostable amylopullulanase producing Clostridium thermosulfurogenes SVM17 was selected for the study. Culture conditions for growth and enzyme production parameters were optimized. Enzyme synthesis occurred at temperatures between 50 and 70°C with an optimum of 60°C. The enzyme production was observed between pH 4.0 and 9.0 with an optimum of pH 7.5. Maximum growth and enzyme production was reached in 12 and 24 h of incubation, respectively. Various raw starches for amylopullulanase production were also studied. The results showed that, different raw starches influenced the enzyme yields to a varied extent. Among them, raw sago starch was found to be the best carbon source followed by wheat and potato starch, respectively on both amylase and pullulanase yields.