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A multi spectral hand-held spatial frequency domain imaging system for imaging human colorectal cancer

A multi-spectral, portable, hand-held LED based spatial frequency domain imaging system was used for ex vivo imaging pretreatment and post treatment human colon and rectal tissues. Freshly excised human colon and rectal tissue samples were imaged with the hand-held SFDI probe with 9 wavelengths extending from visible to NIR (660-950 nm). Important tumor biomarkers such as hemoglobin, scatter amplitude, scatter spectral slope, water and lipid content were quantitatively extracted from the SFDI absorption and scattering images. Significant differences were observed between the absorption as well as scattering distribution of normal, tumor and polyp tissue as well as between pretreated and post-treated tumors.