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Removal of excess chlorazol black stains from microcrustacean specimens


The preparation of specimens is a crucial aspect of zoological studies. Some specific study techniques or certain taxonomic groups require staining processes. However, an excessive exposure to the staining agents can turn a specimen improper for morphological studies. Here, we report that an excess of chlorazol black, one of the most used stains in biology, can be easily removed from crustacean specimens with a solution of methanol and Extran® detergent or, less effectively, with a solution of methanol and commercial dishwashing soap.