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Increased proliferation of neuroglia and endothelial cells in the supraoptic nucleus and hypophysial neural lobe of young rats drinking hypertonic sodium chloride solution

Male rats aged 30 to 35 days were provided with 1.75% sodium chloride solution as drinking fluid for two weeks, to create a chronic increase in plasma osmolality. During this time, the animals and a control group of water‐drinking rats received two injections per day of 1 μCi of 3H‐thymidine per gram body weight to label dividing cells and their progeny. At the end of this period, the animals were sacrificed by aldehyde perfusion. Sections 1 μm‐thick were saved at intervals from a series cut throughout the length of the supraoptic nucleus; these sections and others taken from the caudate nucleus and the neural lobe of the hypophysis were processed for radioautography.