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[Supercritical CO2 fluid extraction of Rosmarinus officinalis and capability of extracts eliminate OH radical].

OBJECTIVE
Supercritical CO2 fluid extraction process of antioxidation active components from Rosmarinus officinalis was studied.


METHOD
A new extraction process of components extracted from R. officinalis by supercritical CO2 fluid extraction (SFE-CO2 ) was studied in detail. The capability of that the extract eliminate *OH radical was tested by electron paramagnetic resonance (EPR) technique and spin catch technique.


RESULT AND CONCLUSION
With free radical clearance as index, by range and variance analysis, the optimum extraction process conditions were: keeping pressure at 30 MPa and temperature at 75 degrees C for 1 h, in the same time adding alcohol 0.30 mL x g(-1).