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An AFM-based study of porcine OBP film for artifical olfaction

The odorant binding protein (OBP) plays an important role in olfactory signal transmission. This paper studies the potential application of OBP on bionic artificial nose. Above all, OBP is immobilized in solid phase. So two kinds of OBP film were prepared. Firstly, OBP is covalently immobilized on the silicon wafer using DITC as a crosslinker. Secondly, polypyrrole film was fabricated on the ITO glass by the electrochemical polymerization. The topograph of these films was measured using an Atomic Force Micrograph (AFM). In order to evaluate the affinity of OBP and its antibody, the AFM probe was coated with the anti-OBP using the covalent method. The force-distance curve, acquired by the AFM instrument, was analyzed to reveal the characteristics of thin film and the binding property between OBP and antibody.