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Determination of Forsythiaside A and Phillyrin of Forsythia Suspensa Leaves Processed by Different Methods

Objective To determine the content of forsythiaside A and phillyrin of Forsythia suspensa leaves processed by different methods. Methods HPLC was used to determine the content of forsythiaside A and phillyrin, the column employed was BDS Hypersil-C18(4.6 mm×150 mm, 5μm); the mobile phase for separation of forsythiaside A was acetonitrile-0.4 % acetic acid(15:85); as for phillyrin, the mobile phase was acetonitrile-water(25:75);the detection wavelength for forsythiaside A and phillyrin were 330 and 277 nm, respectively; the column temperature was 40 ℃; the flow rate was 0.8 m L/min; the injection volume was 10 μL. Results The contents of forsythiaside A and phillyrin of Forsythia suspensa leaves processed by different metods were high, and the content order was: steaming and oven drying>frying and oven drying>steaming and sun drying>frying and sun drying>oven drying>sun drying. Conclusion The content of forsythiaside A and phillyrin of Forsythia suspensa leaves are influenced by different processing methods. Steaming or frying and oven drying are beneficial to conservation of forsythiaside A and phillyrin, oven drying is more beneficial to conservation of forsythiaside A and phillyrin than sun drying.