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Comparison of different methods for coagulase production by staphylococci

Aim: To compare different methods for detection of Coagulase production by Staphylococcus aureus. Material and methods: Total 76 species of Staphylococci were isolated from 1083 clinical specimens. Coagulase tests were performed using five different methods including Slide Coagulase test, Tube Coagulase test with diluted human plasma (I), undiluted human plasma (II) and Brain heart infusion (BHI) broth with undiluted human plasma (III) and Coagulase mannitol agar plate method as per standard methods. Results: In Slide Coagulase test, 43 (86%) out of 50 strains gave positive results. In all the three methods of Tube Coagulase test, 50 (100%) out of 50 strains gave positive results. In Coagulase mannitol agar plate method, 48 (96%) out of 50 strains produced opaque zones which indicated coagulase production. Conclusion: This study shows that, in all the five different methods, all three Tube methods gave 100% positive results. Slide coagulase test gave 43 (86%) out of 50 positive results.