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Molecular basis of cullin-3 (Cul3) ubiquitin ligase subversion by vaccinia virus protein A55

BTB–Kelch proteins are substrate-specific adaptors for cullin-3 (Cul3) RING-box–based E3 ubiquitin ligases, mediating protein ubiquitylation for subsequent proteasomal degradation. Vaccinia virus encodes three BTB–Kelch proteins: A55, C2, and F3. Viruses lacking A55 or C2 have altered cytopathic effects in cultured cells and altered pathology in vivo. Previous studies have shown that the ectromelia virus orthologue of A55 interacts with Cul3 in cells. We report that the N-terminal BTB-BACK (BB) domain of A55 binds directly to the Cul3 N-terminal domain (Cul3–NTD), forming a 2:2 complex in solution. We solved the structure of an A55BB/Cul3–NTD complex from anisotropic crystals diffracting to 2.3/3.7 Å resolution in the best/worst direction, revealing that the overall interaction and binding interface closely resemble the structures of cellular BTB/Cul3–NTD complexes, despite low sequence identity between A55 and cellular BTB domains. Surprisingly, despite this structural similarity, the affinity of Cul3–NTD for A55BB was stronger than for cellular BTB proteins. Glutamate substitution of the A55 residue Ile-48, adjacent to the canonical φX(D/E) Cul3-binding motif, reduced affinity of A55BB for Cul3–NTD by at least 2 orders of magnitude. Moreover, Ile-48 and the φX(D/E) motif are conserved in A55 orthologues from other poxviruses, but not in the vaccinia virus proteins C2 or F3. The high-affinity interaction between A55BB and Cul3–NTD suggests that, in addition to directing the Cul3–RING E3 ligase complex to degrade cellular/viral target proteins that are normally unaffected, A55 may also sequester Cul3 from cellular adaptor proteins, thereby protecting substrates of these cellular adaptors from ubiquitylation and degradation.

Vaccinia virus (VACV) 2 is a dsDNA virus in the Orthopoxvirus genus of the Poxviridae. Historically, VACV was used as the vaccine to eradicate smallpox (1). Its genome contains ϳ200 genes, about half of which are involved in the modulation of host immune response to viral infection, and the virus has been used as a model system to study innate immunity (2). The mechanisms by which several VACV proteins act to inhibit innate immune sensing and effector function, especially those involved in the inhibition of NF-B signaling, have been well characterized (2,3). Nevertheless, many VACV immunomodulatory proteins are still poorly understood, and one such protein is A55.
A55 is an intracellular protein encoded by the A55R gene of VACV (4). It belongs to the BTB (Bric-a-brac, Tramtrack, and Broad complex)-Kelch protein family, which are substrate adaptor proteins specific for the cullin-3 (Cul3)-RING (Really Interesting New Gene)-based E3 ubiquitin ligase (C3RL) complex (5). The N-terminal region of these proteins contains a BTB domain that mediates dimerization and binding to Cul3, a three-box helical bundle region, and a BACK (for BTB and C-terminal Kelch) domain that is likely responsible for correctly orienting the C terminus (5)(6)(7)(8)(9)(10)(11)(12)(13). The C-terminal region comprises 4 -6 Kelch repeats arranged into a single ␤-propeller that captures the substrates for the C3RL complex; alternatively, these Kelch domains may also interact with actin filaments to regulate cytoskeleton organization (5, 9 -11, 14 -19). In cells, there are many BTB domain-containing proteins conjugated with different substrate recognition domains, and their interactions with various substrates and C3RL complexes are implicated in several cellular processes, including protein degradation, transcriptional regulation (KEAP1), the gating of voltage-gated potassium channels (KCTDs), and cytoskeleton modulation (KLHLs) (19 -25). Apart from mimiviruses, poxviruses are the only family of viruses that make BTB domaincontaining proteins (26 -30).
Deletion of A55 from VACV does not diminish virus replication in cultured cells (4). However, cells infected with VACV lacking A55 (v⌬A55) demonstrated altered cytopathic effects, including the loss of Ca 2ϩ -independent cell adhesion and cellular projections, suggesting that A55 plays a role in the modulation of the cytoskeleton (4). The use of an intradermal murine model of infection demonstrated that infection with v⌬A55 caused increased lesion size compared with WT virus, suggesting that A55 plays a role in altering the host immune response in vivo (4).
VACV encodes three BTB-Kelch proteins, namely A55, C2, and F3. Despite having similar domain organizations, A55 shares limited sequence identity with C2 and F3 (22 and 25%, respectively). Like A55, C2 and F3 are dispensable for VACV replication in cultured cells (31,32). Infection of cells with v⌬A55 or with VACV lacking C2 (v⌬C2) produced a similar loss of Ca 2ϩ -independent cell adhesion, suggesting that A55 and C2 affect similar cellular pathways (4,31). However, intradermal infection in vivo with v⌬C2 resulted in similar-sized lesions to WT infection, but these lesions persisted longer, distinct from the phenotype observed for v⌬A55 (4,31). Infection with VACV lacking F3 (v⌬F3) produced no distinct phenotype in cultured cells, but intradermal infection yielded smaller lesions compared with WT virus (32). These results suggest that VACV BTB-Kelch proteins are functionally divergent despite having a conserved domain organization.
C3RLs are a family of multimodular cullin-RING-based E3 ubiquitin ligases that recruit substrates specifically via BTB domain-containing adaptor proteins (5,6). Cul3, the all-helical stalk-like scaffold subunit of C3RLs, interacts directly with BTB domain-containing proteins via its N-terminal domain (6 -8, 13, 24, 33). The C-terminal domain of Cul3 interacts with the RING-based E3 ligase protein to recruit the ubiquitin-loaded E2-conjugating enzyme for substrate ubiquitylation and is dispensable for binding to BTB domain proteins (5,11,34). Crystal structures of several cellular BTB domain proteins in complex with the Cul3 N-terminal domain (Cul3-NTD) have been reported (6,7,13,24). These structures revealed a unique mode of binding of BTB-containing adaptor proteins to the C3RL family of E3 ubiquitin ligases. Interaction with Cul3 is mainly via the BTB domain, with additional contacts from the threebox region, whereas the BACK domain does not participate in the binding. The N-terminal 22 residues of Cul3 (N-terminal extension (NTE)) are usually disordered and dispensable for binding, and many reported binding studies of BTB domain-containing proteins to Cul3 were carried out with N-terminally truncated Cul3-NTD (Cul3 20 -381 for KLHL3, SPOP, and KCTD5, Cul3  for KLHL11, and Cul3 26 -381 for KEAP1) (6,7,13,24). However, the Cul3-NTE does provide extra hydrophobic contacts with the three-box region upon binding to KLHL11 and KCTD5, resulting in significant increases in affinity (6,25).
The ectromelia virus (ECTV) orthologue of A55, EVM150, shares 93% sequence identity to A55. EVM150 has been reported to interact with Cul3 via its BTB domain and co-localizes with the C3RL and conjugated ubiquitin in cells (43). In addition, the BTB domain of EVM150 was reported to inhibit NF-B signaling, although Cul3 appeared dispensable for this activity (42).
In this study, we showed that A55 binds directly to Cul3 and solved the crystal structure of a complex between Cul3-NTD and the BTB-BACK (BB) domain of A55. Although the overall conformation of the complex is similar to reported cellular BTB/Cul3-NTD structures, Cul3-NTD binds A55BB more tightly than it does cellular BTB proteins. This strong A55/Cul3 interaction may allow VACV to redirect the E3 ubiquitin ligase complex to degrade novel target proteins and/or to subvert cellular BTB/Cul3-NTD interactions to rescue proteins from degradation.

A55 binds to Cul3 of the E3 ubiquitin ligase complex via its N-terminal BB domain
Poxvirus BTB-Kelch protein EVM150 has been shown to co-precipitate with Cul3 and modulate innate immune responses upon infection (42,43). A55 is also predicted to have a BTB-Kelch domain architecture and shares 93% aa identity to EVM150. To test whether A55 interacts with Cul3, co-immunoprecipitation experiments were performed using inducible HEK293T-REx cell lines expressing A55 with a FLAG-containing tandem affinity purification (TAP) tag at its N terminus (TAP-A55) or B14, an NF-B inhibitor from VACV (44), with a C-terminal TAP tag (B14 -TAP). Endogenous Cul3 co-immunoprecipitated with TAP-A55, but not B14 -TAP, when overexpressed in HEK293T-REx cells (Fig. 1A). This suggests that Cul3 specifically co-immunoprecipitates with A55 and not with other VACV immune modulatory proteins. Furthermore, TAP-A55 co-immunoprecipitated with N-terminally Myc-tagged Cul3 (myc-Cul3) but not with myc-Cul5, suggesting that A55 interacts specifically with Cul3 and not with other cullin family proteins (Fig. 1B). To dissect the region of A55 that binds to Cul3, the BB and Kelch domains of A55 were tagged at the N terminus with TAP and were immunoprecipitated after overexpression in HEK293T-REx cells. Endogenous Cul3 co-immunoprecipitated with the N-terminal BB domain but not with the C-terminal Kelch domain (Fig. 1C). These results suggest that, like the ECTV BTB-Kelch protein EVM150, A55 interacts with Cul3 and that this interaction is mediated solely by the N-terminal BB domain.

A55 is an obligate dimer in solution and forms a 2:2 complex with Cul3
Previous biochemical and structural analysis has shown that Cul3 binds cellular BTB-Kelch proteins via its N terminus (residues 1-388), while its C terminus (389 -767) is not required for binding (6 -8, 13). To test whether A55 forms a direct complex with Cul3 in solution, the A55 BB domain (A55BB, residues 1-250) and the Cul3 N-terminal domain (Cul3N⌬22, residues 23-388) were expressed in Escherichia coli and purified according to protocols described under "Experimental procedures." Size-exclusion chromatography coupled to

Structure of Cul3 in complex with vaccinia virus protein A55
multiangle light-scattering (SEC-MALS) studies together with SDS-PAGE analysis showed that A55BB exists as a homodimer in solution (expected molecular mass 60 kDa) (Fig. 1D). This is consistent with observations for other cellular BTB proteins (6)(7)(8). Cul3N⌬22 is monomeric (expected molecular mass 46 kDa). However, when A55BB and Cul3N⌬22 were mixed at ϳ1:1 molar ratio, a complex was formed with an apparent molecular mass of 141 kDa, consistent with a 2:2 complex of A55BB/Cul3N⌬22 (expected molecular mass 152 kDa) (Fig. 1, D and E). Overall, the results show that A55 is dimeric in solution and binds directly to Cul3 to form a heterotetramer.

A55 binds to Cul3 with low-to sub-nanomolar affinity
Isothermal titration calorimetry (ITC) experiments were carried out to determine the binding affinity between A55BB and Cul3. Two different truncations of Cul3 containing the N-terminal domain were used: Cul3N (residues 1-388) and Cul3N⌬22 (residues 23-388) to compare the binding affinities between A55 and Cul3 with or without the Cul3-NTE. A55BB formed equimolar complexes with both Cul3N⌬22 and Cul3N with affinities in the low nanomolar (5.3 Ϯ 2.9 nM) and subnanomolar (Ͻ1 nM) range, respectively (Fig. 2, A and B; Table  1). The binding affinity of A55BB for Cul3N could not be deter-mined accurately as rapid depletion of free Cul3N in the cell upon the introduction of A55BB prevented fitting of the resultant titration data to a single-site binding model. Attempts to lower the concentrations of A55BB and Cul3N or to use displacement titration experiments (45) were unsuccessful due to limitations of instrument sensitivity. Previous studies have shown cellular BTB proteins to bind the Cul3 N-terminal domain with much lower affinities than observed for A55BB (6 -8, 13). To facilitate a direct comparison, the binding of KLHL3-BB to Cul3N and Cul3N⌬22 was measured by ITC ( Fig.  2, C and D). These experiments confirmed that the affinity of Cul3-NTD for A55BB is ϳ10-fold tighter than for KLHL3-BB (Fig. 2, C and D; Table 1). Interestingly, the affinity of A55BB for Cul3N⌬22 was stronger despite the enthalpic contribution to the interaction (⌬H ϭ Ϫ9.9 Ϯ 0.8 kcal/mol) being lower than for the equivalent interaction between KLHL3-BB and Cul3N⌬22 (⌬H ϭ Ϫ18.9 Ϯ 2.0 kcal/mol) ( Table 1). This suggests that the tighter interaction arises from a more favorable entropic contribution upon complex formation, such as the burial of exposed hydrophobic regions leading to the release of ordered solvent molecules and/or less conformational restriction of A55 upon complex formation. Taken together, the ITC data presented here show that the VACV A55BB

Structure of Cul3 in complex with vaccinia virus protein A55
binds Cul3-NTD more tightly than previously-studied cellular BTB domain-containing proteins (Table 1) and that different thermodynamic properties of the interaction contribute to this enhanced binding.

Determination of the A55BB/Cul3N⌬22 complex structure
To understand the molecular mechanism underlying the observed high-affinity interaction between A55BB and Cul3-NTD, the A55BB/Cul3N⌬22 complex was purified and sub-jected to extensive crystallization screening for structural characterization. Initial trials did not yield any crystals. To promote crystallization, A55BB was subjected to surface entropy reduction by reductive methylation (46) before being purified in complex with Cul3N⌬22 (Fig. S1). Crystals of methylated (M) A55BB in complex with Cul3N⌬22 grew as thin needles after 2 weeks. By using microseeding and varying the pH and concentration of the precipitants, optimized crystals were grown that diffracted to 2.3 Å in the best direction. Inspection of the dif-

Structure of Cul3 in complex with vaccinia virus protein A55
fraction data suggested severe anisotropy, with significantly worse diffraction along one axis (3.7 Å in direction 0.76 a* Ϫ 0.65 c*) compared with the other major axes (2.6 Å in the direction b* and 2.3 Å in the direction 0.92 a* ϩ 0.39 c*), so these data were processed with anisotropic scaling and truncation using STARANISO (47) and DIALS (48). The final processed dataset contained 23,509 unique reflections (Table 2), equivalent to the number of reflections expected for a 2.8 Å dataset collected from an isotropically-diffracting crystal with equivalent space group and unit cell dimensions. The anisotropy of diffraction was present in all crystals of the A55BB(M)/Cul3N⌬22 complex for which data were collected (Ͼ20 individual crystals).
The structure of A55BB(M)/Cul3N⌬22 crystal was solved by molecular replacement using B-cell lymphoma 6 BTB domain (PDB code 1R29) (49) and Cul3 20 -381 from the SPOP/Cul3 complex structure (PDB code 4EOZ) (13) as the search models. Although most of the Cul3N⌬22 molecule could be modeled with ease, the initial map for A55BB was less well-defined with relatively weak density for the three-box and BACK regions. Anisotropic scaling of the diffraction data and the use of interactive molecular dynamics in ISOLDE (50) improved the model quality and fit to density significantly. The final model was refined using BUSTER (51) and has residuals R work /R free of 0.266/0.282 with good overall geometry. Data collection and refinement statistics are summarized in Table 2. No crystals of A55BB(M) either alone or in complex with Cul3N could be obtained despite extensive crystallization trials.

Table 1 Comparison of the dissociation constants (K D ), enthalpic change (⌬H), and entropic change (T⌬S) for BB-Cul3 interaction
Experiments for this study were performed at least twice, and mean Ϯ S.E. is shown.

Structure of Cul3 in complex with vaccinia virus protein A55
Overall, the A55BB/Cul3N⌬22 complex closely resembles other structures of Cul3 in complex with cellular BTB domaincontaining proteins (Fig. 3C). The A55-binding interface of Cul3-NTD is formed primarily of residues in helices ␣2 and ␣5, with extra contacts from the ␣1-␣2 loop and from the C termi-nus of ␣3. Residues at the Cul3-binding interface of A55BB are primarily found in the BTB domain with additional contacts in the three-box region; the BACK domain does not contribute to the interaction (Fig. 4). This mode of interaction is consistent with the KLHL3/Cul3 (7), KLHL11/Cul3 (6), and KEAP1/Cul3

Structure of Cul3 in complex with vaccinia virus protein A55
(PDB code 5NLB) complex structures (Fig. S2). Analyses of the interface areas and the number of interface residues for A55 and all available BTB/Cul3 complex structures showed no striking overall differences (Fig. S2). However, compared with other BTB/Cul3-NTD structures, the A55BB/Cul3 interface has more hydrogen bonds and a reduced contribution from hydrophobic interactions (Fig. S2).
Only 196 of the 250-aa residues that comprise A55BB could be modeled confidently; the density for side chains in the last modeled helix of the BACK domain (␣10, residues 180 -196) is weak compared with density for side chains at the BTB/Cul3 interface (Fig. S3, A and B), and density for BACK domain residues 197-250 was not sufficiently well resolved to be modeled unambiguously (Fig. S3C). Correspondingly, the B factors of A55 residues at the Cul3-binding interface are lower than in the BACK domain (Fig. S3D). Inspection of the crystal lattice shows large solvent channels next to the BACK domain, and this lack of crystal contacts at the C terminus is likely to account for the poor density observed in this region (Fig. S3, C and E). When superimposing KLHL11 and KLHL3 onto different regions of A55, inter-domain flexibility is evident (Fig. S4, A-D and E-F,  respectively). Two pivot points in the structure can be found: the BTB ␣5-␣6 helix-turn-helix and the three-box region, respectively; the latter appears to be the major pivot point around which the BTB and BACK domain rotate relative to each other (Fig. S4, I-K). When measuring the angles between different subdomains (BTB ␣1-4 , BTB ␣5-␣6 , three-box, and BACK), the angle formed by BTB ␣5-␣6 -three-box-BACK in A55 is much larger compared with the corresponding angles in KLHL3 and KLHL11, thus rendering A55 more linear across the BB domain than KLHL3 and KLHL11 (Fig. S4, I-K).
Crystals of the A55BB/Cul3N⌬22 could be obtained only when the A55BB protein had been methylated in vitro. Although there was density consistent with the presence of addi-tional atoms adjacent to the amino groups of two lysine side chains (Lys-36 and Lys-132), they were not sufficiently well resolved to allow modeling of the methyl groups (Fig. S5A). Only one lysine (Lys-136) was found at the binding interface (Fig. S5B), and ITC studies showed that methylated A55BB binds to Cul3 with affinity similar to unmodified A55BB (Fig.  S5, C and D).

Hydrophobic interaction at A55 residue 48 is required for highaffinity binding to Cul3
Despite similarity in the overall structures, A55BB binds Cul3-NTD with much higher affinity than other BTB proteins. It has been suggested the key determinant for the interaction with Cul3 is a conserved X(D/E) motif found in the ␣3-␤4 loops of the BTB domain, where is a hydrophobic residue, and X is any residue (6,7,13). This motif exists in A55, corresponding to residues Phe-54 (), Ile-55 (X), and Asp-56 (D/E), respectively (Figs. 4 and 5B). As in KLHL3, KLHL11, and SPOP, the side chain of residue (Phe-54) in A55 is buried in a hydrophobic cavity on the surface of Cul3 (Fig. 5, C-F). Mutation of the residue in SPOP to a charged residue (M233E) completely abolished binding to Cul3, highlighting the significance of the residue for the interaction (13). An F54E mutant of A55BB was purified and shown to have similar thermal stability to the WT protein (Fig. 6, A and B). ITC analysis demonstrated that the F54E mutation reduces the affinity of A55BB for Cul3N⌬22 and Cul3N by at least 10-fold compared with the WT protein, yielding dissociation constants (K D ) similar to those of cellular BTB proteins (Fig. 6, C and D; Table 3). This suggests that residue Phe-54 of A55 is involved in the interaction but is not critical for binding to Cul3. The A55 residue Asp-56, equivalent to the D/E residue of the X(D/E) motif, forms side chain and backbone hydrogen bonds with Cul3 residues Ser-53 and Phe-54, respectively. A D56A mutation was introduced into A55, but the

Structure of Cul3 in complex with vaccinia virus protein A55
mutant could not be purified following bacterial expression, suggesting Asp-56 is critical for the correct folding of A55.
As the X(D/E) motif was not absolutely required for binding of A55 to Cul3, the contribution of other residues was investigated. A hydrogen bond is formed between Asp-99 of A55 and Tyr-125 of Cul3, and this interaction is conserved in KLHL11 and SPOP but not in KLHL3 (Fig. 5G). Mutation at this site only caused moderate reduction in affinities for both Cul3N⌬22 and Cul3N (Fig. 6, E and F). Residue Ile-48 of A55 is adjacent to the residue Phe-54 and, like Phe-54, the side chain of Ile-48 extends into a hydrophobic cleft on the Cul3 surface (Fig. 5C). This residue is not conserved in KLHL3, KLHL11, or SPOP, and the equivalent residues (Ala-77, Pro-124, and Ala-227, respectively) have smaller side chains and form less extensive interactions (Fig. 5, D-F). Two mutant forms of A55BB were generated, A55BB-I48E and A55BB-I48A, and both were

Structure of Cul3 in complex with vaccinia virus protein A55
shown to have similar thermal stability to the WT protein (Fig.  6, A and B). A55BB-I48A retained high affinity for Cul3N⌬22 and Cul3N (Fig. 6, G and H). However, the I48E substitution reduced Cul3N⌬22 binding to levels undetectable by ITC and reduced the affinity for Cul3N by at least 2 orders of magnitude (Fig. 6, I and J), to well-below those of the cellular BTB proteins for Cul3-NTD (Tables 1 and 3). The residual binding of A55BB-I48E to Cul3N is likely to be mediated via contacts with Cul3N-NTE, as has been characterized previously for KLHL11 (6).

An interaction between a poxvirus BTB-Kelch protein and
Cul3 has been demonstrated previously for the ECTV protein EVM150 by co-immunoprecipitation from transfected cells (42,43). Cellular BTB proteins have been reported to bind directly to Cul3 (6 -8, 13, 24, 25, 33). However, because of the low sequence identity (20 -25%) between poxvirus and cellular BTB proteins (Fig. 4), it was unclear whether EVM150 and other poxvirus BTB-Kelch proteins would bind Cul3 in a similar manner. Here, we show that VACV BTB-Kelch protein A55, a close orthologue of EVM150, also binds to Cul3, and this interaction is direct in nature. Surprisingly, the binding of Cul3 to A55BB is much tighter than to human BTB domains ( Fig. 2 and Table 1). To understand the molecular basis of this tight interaction, the crystal structure of the A55BB/Cul3N⌬22 complex was determined using anisotropic diffraction data extending to 2.3 Å (with an observation/parameter ratio equivalent to that of an isotropic 2.8 Å resolution structure). This is the first reported crystal structure of a virus BTB-Kelch protein in complex with the E3 ubiquitin ligase scaffold protein Cul3.
The overall conformation of the A55BB/Cul3-NTD complex resembles closely the structures of other cellular BTB/ Cul3-NTD protein complexes, with a similar mode of dimerization and a conserved Cul3-binding interface despite low sequence identities (Fig. 3, C-E; Fig. S2). The interface area and the number of interface residues at the A55BB/Cul3N⌬22binding interface are comparable with cellular BTB/Cul3-NTD interfaces (Fig. S2, inset table). The conserved X(D/E) motif, which was found to be a key contributor to the interaction between SPOP and Cul3, is conserved in A55 (Figs. 4 and 5B) (13). However, mutation of Phe-54 to glutamate at the position in A55BB only moderately reduced its affinity for Cul3 (Fig. 6, C and D), whereas the equivalent mutation in SPOP resulted in complete loss of binding (13). A55 residue Ile-48, adjacent to Phe-54, makes more extensive contacts with Cul3 than the equivalent residues in cellular BTB proteins (Fig. 5, C-F). Substitution of Ile-48 to alanine caused only a modest decrease in affinity (Table 3), demonstrating that the additional hydrophobic interactions mediated by isoleucine at this position are not the sole determinants of higher-affinity Cul3 binding by A55BB. However, substitution of Ile-48 to glutamate weakened the interaction with the Cul3 N-terminal domain lacking the NTE (Cul3N⌬22) such that it could no longer be detected by ITC, and reduced the affinity for the full Cul3 N-terminal domain (Cul3N) by at least 2 orders of magnitude (Fig. 6, G and H; Table 3). An equivalent mutation (A77E) in KLHL3 similarly reduced the affinity for Cul3N⌬20 to levels undetectable by ITC (7). Taken together, these results confirm that hydrophobic interactions at this position, adjacent to the conserved X(D/E) motif, are necessary for binding of BTB proteins to Cul3. Interestingly, ITC studies suggest that the A55BB/Cul3-NTD interaction is more entropically favorable than the KLHL3-BB/Cul3-NTD interaction ( Fig. 2 and Table  1), whereas structural analysis suggests that hydrophobic interactions contribute less energy (via the entropically-favorable release of solvent) to the A55/Cul3 interaction (Fig. S2, inset  table ). In Cul3-bound KLHL3, KLHL11, and SPOP structures, the ␣3-␤4 loops, which contain the residue and interact with hydrophobic pockets on the Cul3 surface, adopt helical conformations (helix ␣3.1, Fig. 5, B and D-F). In A55, the residue Phe-54 also fits into a deep hydrophobic pocket on the surface of Cul3, but the ␣3-␤4 loop of A55 does not adopt a helical conformation (Fig. 5, A and C). Interestingly, in the structures of unbound KLHL11 and SPOP, the ␣3-␤4 loop containing the residue is less well ordered and has a different conformation (6,8). Such structural rearrangement upon binding to Cul3 would present an entropic penalty to binding. It is tempting to speculate that a lack of such a ␣3-␤4 loop rearrangement, rather than the burial of exposed hydrophobic regions, contributes to the entropically-favorable tight binding of A55BB to Cul3-NTD. Furthermore, such structural rearrangement may not occur in the absence of the favorable hydrophobic interaction mediated by the residue, suggesting a mechanism by which binding to Cul3 would be more significantly diminished for SPOP than for A55 when this residue was mutated.
Sequence alignments reveal the BB domains of A55 and other orthopoxvirus BTB-Kelch orthologues such as EVM150 to share extensive (Ͼ77%) identity (Table 4), including conservation of the residues in the X(D/E) motif and Ile-48 (Fig. 7). This strongly suggests that the interaction between A55 orthologues and Cul3 is conserved among poxviruses. In contrast, most of the Cul3-binding residues of A55 are not conserved in the other two VACV BTB-Kelch proteins, C2 and F3 (Fig. 7), which share little sequence identity to A55 (25 and 23%, respectively). This suggests that these proteins are unlikely to interact with Cul3, despite being classified as BTB-Kelch proteins, and is consistent with these proteins being functionally distinct (4,31,32). The N-terminal dimerization helix of the BTB domain appears to be missing in C2 (Fig. 7) suggesting that, unlike most BTB-Kelch proteins, C2 may not be able to form homo-or heterodimers via the same mechanism as A55.
In vivo, VACV expressing A55 induced a smaller lesion in a murine model of intradermal infection compared with a virus lacking A55 (4). In cells, the BTB domain of the ECTV EVM150 Table 3 Comparison

of the dissociation constants (K D ) for WT and mutant A55BB for Cul3
Experiments were performed n times and the mean Ϯ S.E. is shown.

Structure of Cul3 in complex with vaccinia virus protein A55
was reported to inhibit TNF␣-induced NF-B activation; however, this inhibition appears to be Cul3-independent (42). The interaction between A55 and Cul3 therefore is unlikely to be relevant for the inhibition of NF-B signaling. As a scaffold protein for an E3 ubiquitin ligase complex, Cul3 not only interacts with the BTB-Kelch family of adaptor proteins but also other BTB domain-containing adaptor proteins such as BTB zinc-finger proteins, MATH-BTB proteins (where MATH is Meprin and TRAF homology domain), small RhoBTB GTPases, and KCTD proteins (5,8,10,13,20,24,52). The outcome of the interaction will depend on the specific substrates recruited by the BTB adaptor proteins and will regulate a diverse range of cellular processes, including hypoxic response, ion-channel gating, as well as cytoskeleton organization (19,53,54). The fact that A55BB is able to bind Cul3-NTD with much stronger affinity than reported, cellular binding partners suggest two possible functions of A55. First, A55 may bind to Cul3 and redirect the E3 ubiquitin ligase complex to ubiquitylate otherwise untargeted proteins for proteasomal degradation. Alternatively, A55 may sequester Cul3 and prevent the ubiquitylation and/or proteasomal degradation of proteins that are normally ubiquitylated/degraded upon viral infection. Further experiments are required to discriminate whether A55 fulfills either or both of these roles during infection.

Structure of Cul3 in complex with vaccinia virus protein A55
A55BB binding to the Cul3 N-terminal domain is significantly increased by the presence of the N-terminal 22 amino acids of Cul3 (Table 1), and the I48E mutant lacks the ability to bind Cu3N⌬22 yet can bind Cul3N with nanomolar affinity (Table 3). This NTE of Cul3 has been shown to interact extensively with a hydrophobic groove formed primarily by the three-box region of KLHL11 (Fig. S6A) (6). Comparison of the structures of KLHL11 in complex with Cul3N or Cul3N⌬22 shows this groove to be pre-formed, rather than being induced by NTE binding (Fig. S6, A and B). However, sequence and structural alignment of A55 and KLHL11 suggest that an equivalent hydrophobic groove is not present on the surface of A55 ( Fig. 4 and Fig. S6C). Indeed, the BTB, three-box, and BACK domains are arranged in linear fashion in A55, whereas they form a crescent in KLHL11 or KLHL3 (Fig. S4, I-K). It is therefore likely that A55 binds the Cul3-NTE via a different set of interactions. Crystallization trials of A55 with Cul3 containing the NTE region (Cul3N) have to date been unsuccessful, and further studies are thus needed to identify residues key for the interaction between A55 and the Cul3-NTE.

Conclusion
The structure of the first virus BTB-Kelch protein in complex with Cul3 is presented here, which has provided insight into how poxviruses may utilize the host Cul3-based E3 ubiquitin ligase complex for its own benefit. A55 binds Cul3 with much stronger affinity than cellular BTB-Kelch proteins. A single point mutation in A55, I48E, significantly diminishes Cul3 binding and could be exploited by future studies to probe the contribution of the A55-Cul3 interaction to VACV virulence.

Protein expression and purification
WT and mutant A55BB and Cul3N⌬22 were expressed in B834(DE3) E. coli cells (Novagen), and Cul3N and KLHL3 were expressed in Rosetta2(DE3)pLysS E. coli cells (Novagen). Bacteria were grown at 37°C in 2ϫ TY medium with shaking at 200 rpm to an A 600 of 0.7-0.9, whereupon protein expression was induced by either adding 0.2 mM IPTG and incubating at 37°C for 4 h (Cul3N) or by cooling the cultures to 22°C, adding 0.2 mM IPTG, and incubating for 4 h (Cul3N⌬22) or overnight (WT and mutant A55). Cells were harvested by centrifugation at 5,000 ϫ g for 15 min, and pellets were stored at Ϫ80°C.
Cells were thawed and resuspended in lysis buffer containing 20 mM HEPES, pH 7.5, 500 mM NaCl, 1 mM ␤-mercaptoethanol, 0.05% Tween 20, 0.5 mM MgCl 2 , 400 units of bovine DNase I (Roche Applied Science), and 200 l of EDTA-free protease inhibitor mixture (Sigma). Cells were lysed by passage through a TS series cell disruptor (Constant Systems) at 24,000 p.s.i. Lysates were collected and cleared by centrifugation at 40,000 ϫ g for 30 min at 4°C. Cleared lysates were applied to a 5-ml HiTrap TALON crude column (GE Healthcare) preequilibrated with binding buffer (20 mM HEPES, pH 7.5, 500 mM NaCl, 5 mM ␤-mercaptoethanol) to capture the His 6 -tagged proteins. The column was washed with binding buffer, and the bound proteins were eluted with a gradient of 10 -150 mM imidazole in binding buffer. Eluted proteins were pooled, concentrated,

Structure of Cul3 in complex with vaccinia virus protein A55
and further purified by SEC using a Superdex 200 column (GE Healthcare) equilibrated in gel-filtration buffer (20 mM HEPES, pH 7.5, 200 mM NaCl, 1 mM DTT). For Cul3N, an additional anionexchange chromatography purification step was performed by exchanging the protein into 20 mM Tris, pH 7.5, 10 mM NaCl, 1 mM DTT and applying to a Mono Q 5/50 GL column (GE Healthcare) before eluting with a linear gradient of NaCl (10 mM to 1 M). Purified proteins were concentrated, snap-frozen in liquid nitrogen, and stored at Ϫ80°C. A55BB migrates more rapidly than expected in SDS-PAGE; peptide mass fingerprinting was used to confirm the identity and integrity of the purified protein.

Size-exclusion chromatography coupled to multiangle light scattering (SEC-MALS)
SEC-MALS experiments were performed at room temperature. For each experiment, 100 l of protein at 3 mg/ml was injected onto a Superdex 200 increase 10/300 GL column (GE Healthcare) pre-equilibrated with 20 mM HEPES, 150 mM NaCl, and 2 mM DTT at a flow rate of 0.5 ml/min. The static light scattering, differential refractive index, and the UV absorbance at 280 nm were measured in-line by DAWN 8ϩ (Wyatt Technology), Optilab T-rEX (Wyatt Technology), and Agilent 1260 UV (Agilent Technologies) detectors. The corresponding molar mass from each elution peak was calculated using ASTRA 6 software (Wyatt Technology).

Reductive methylation
Reductive methylation was carried out at 4°C using modified protocols from Walter et al. (46). Purified A55BB was diluted to 0.8 mg/ml and dialyzed into buffer containing 50 mM HEPES, pH 7.5 and 250 mM NaCl. The protein was mixed with 20 l/ml of 1 M dimethylamine/borane complex (Sigma) and 40 l/ml of 1% formaldehyde (UltraPure EM grade, Polysciences) and incubated for 2 h at 4°C. This step was repeated once before mixing with an additional 10 l/ml of 1 M dimethylamine/borane complex and incubating overnight at 4°C. The reaction was quenched with 10 l of 1 M Tris, pH 7.5. Methylated A55BB was further purified by SEC using a Superdex 200 10/300 GL column equilibrated in 20 mM Tris, pH 7.5, 200 mM NaCl, and 1 mM DTT before being concentrated, snap-frozen, and stored at Ϫ80°C.

Isoelectric focusing (IEF) gel analysis
The IEF gel analysis was performed at 4°C using a Novex pH 3-7 IEF gel (ThermoFisher Scientific) according to the manufacturer's instructions. Native and methylated A55BB were diluted with MilliQ water to 0.8 mg/ml in a total volume of 5 l and mixed with an equal volume of 2ϫ Novex pH 3-10 IEF sample buffer (ThermoFisher Scientific) before loading onto the IEF gel. The gel was fixed in 12% TCA for 30 min and washed with MilliQ water before staining with InstantBlue Protein Stain (Expedeon).

Differential scanning fluorimetry (DSF)
DSF experiments were performed in 96-well PCR microplates (Axygen Scientific) on a ViiA 7 real-time PCR machine (Life Technologies, Inc.). To each well of the plate, buffer (20 mM HEPES, 200 mM NaCl, 1 mM DTT), protein, and 10ϫ protein thermal shift dye (Applied Biosystems) were mixed at 8:1:1 volume ratio in a final volume of 20 l and a protein concentration of 0.2 g/l. Samples were subjected to thermal denaturation from 25 to 95°C with 1°C increments per 20 s, and real-time fluorescence was recorded. Normalized melt curves were fitted to a biphasic sigmoidal curve using Prism7 (GraphPad Software), and the melting temperatures (T m ) were taken as mid-points of the sigmoids.

Crystallization and data collection
Methylated A55BB was mixed with Cul3N⌬22 at 1:1 molar ratio, and the complex was purified by SEC using a Superdex 200 10/300 GL column (GE Healthcare) in 20 mM Tris, pH 7.5, 200 mM NaCl, 1 mM DTT. The purified complex was concentrated to 16.3 mg/ml, and sitting-drop vapor diffusion experiments were attempted by mixing 100 nl of protein with 100 nl of reservoir (4% (v/v) tacsimate, pH 6.5, 12% (w/v) PEG3350) and equilibrating against 80 l of the reservoir solution at 20°C. Thin needles were observed after 2 weeks. Varying the pH, concentration of the tacsimate, concentration of PEG3350, and the protein/reservoir ratio in the sitting drops gave rise to larger crystals that diffracted to ϳ3.8 Å on Diamond beamline I03. For further optimization, seed stocks for microseeding were generated as described previously (56). Briefly, crystals were crushed and transferred into 50 l of stabilizing solution (original reservoir solution) and vortexed, and seven 5-fold serial dilutions of seed into stabilizing solution were generated. Sitting drops were prepared using 100 nl of protein, 150 nl of reservoir, and 50 nl of seed stock. Eventually, a drop containing 3.29% (v/v) tacsimate, pH 6.5, 9.92% (w/v) PEG3350, and 50 nl of 625-fold diluted seed stock gave rise to crystals that diffracted to 2.3 Å in the best direction on Diamond beamline I04. The crystals were cryoprotected by briefly sweeping through reservoir solution containing 25% (v/v) glycerol and flash-cryocooled by plunging into liquid nitrogen. Diffraction data were collected at 100 K on the Diamond beamline I04. Data were indexed and integrated using DIALS (57) as implemented by the xia2 processing pipeline (58). Because of severe anisotropic diffraction, diffraction data were subject to anisotropic scaling using STARANISO (47) and AIMLESS (59).

Structure determination
The structure of the A55BB(M)/Cul3N⌬22 complex was solved by molecular replacement using PHENIX PHASER-MR (60). An initial search using each domain of the SPOP/Cul3 complex (13) (PDB code 4EOZ) as search models successfully placed one copy of Cul3N⌬22, but no solution corresponding to A55BB was forthcoming. MOLREP (61) from the CCP4 pro-

Structure of Cul3 in complex with vaccinia virus protein A55
gram suite (62) was used to locate the A55 BTB domain using B-Cell Lymphoma 6 BTB Domain (49) (PDB code 1R29) as a search model. The three-box region and the first four helices of the A55 BACK domain (␣9 -␣12) were manually built using COOT (63) with iterative rounds of refinement using Refmac5 (64). The structure was improved by the use of real-time molecular dynamics-assisted model building and map fitting with the program ISOLDE (50), followed by TLS and positional refinement using BUSTER (51). The quality of the model was monitored throughout the refinement process using Molprobity (65).

Bioinformatics and structural analysis
Multiple sequence alignments were performed using Clustal Omega (66) and annotated using ALINE (67). Analyses of the binding interfaces were performed using the PDBePISA webserver (68). Molecular figures were generated using PyMOL (69).