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Development of a hydroxyl radical nanoprobe

A hydroxyl radical nanoprobe has been developed by covalently attaching the dye, coumarin-3-carboxylic acid (CCA), to amine-functionalized polyacrylic acid (AFPAA) nanoparticles. These nanoparticles are referred to as PEBBLEs (Probes Encapsulated By Biologically Localized Embedding). This probe has been shown to be highly specific and sensitive. The formation of hydroxyl radical is monitored by an increase in fluorescence over time.