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Genetic Analysis of Sliding Motility inMycobacterium smegmatis

ABSTRACT A screen for nonsliding mutants of Mycobacterium smegmatis yielded 20 mutants with transposon insertions in themps gene, which is involved in glycopeptidolipid biosynthesis. One mutant had an insertion in a gene predicted to encode a membrane transport protein. All mutants lacked glycopeptidolipids and were unable to form biofilms on polyvinyl chloride.