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Control of lipase-mediated glycerolysis reactions with butter oil in single liquid phase media with 2-methyl-2-propanol

Factors affecting the progress of glycerolysis reactions with butteroil mediated by two lipase preparations (from Pseudomonas sp.) in single liquid phase mixtures containing 2-methyl-2-propanol were evaluated. Within the range of parameters evaluated, the conditions best supporting the formation of monoacylglycerols (MAG) were 25-40% butteroil substrate, 10-20 mg of enzyme/mL of substrate mixture, 35 o C, and 100-150 mg of glycerol/mL of substrate mixture (equivalent to a molar ratio of fatty acyl groups to glycerol of 0.61-0.76). Under these conditions, the percent yield of MAG formation from butteroil was 50-60% (mass fraction) relative to the other acylglycerol species and fatty acids accumulated