{'fraction_non_alphanumeric': 0.02653301886792453, 'fraction_numerical': 0.01179245283018868, 'mean_word_length': 5.126353790613718, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
CONTRIBUTION OF DIFFERENT MECHANISMS TO EXPLAIN THE ANTIHYPERTENSIVE EFFECT OF COCOANOX, A COCOA RICH IN POLYPHENOLS: PP.29.173

Objetives: Cocoa polyphenols have shown antihypertensive effects. We investigate the mechanism involved in the antihypertensive effect of CocoanOX (CCX), a cocoa powder prepared by an industrial procedure to prevent polyphenol degradation. Design and Methods: Male 3-week-old spontaneously hypertensive rats (SHR) were divided into four groups of ten animals that were respectively long-term administered tap water (control), 100 mg/kg/day CCX, 200 mg/kg/day CCX or 400 mg/kg/day CCX. Five 20-week-old rats of each group were sacrificed and the different treatments were then removed in the remaining rats. Arterial blood pressure was weekly measured from the 6th to the 24th week of life in the SHR and all the rats were sacrificed at the end of the experimental period. The following measurements were performed in the sacrificed SHR: endothelium-dependent aorta relaxation to acetylcholine, plasma malonildialdehyde, angiotensin converting enzyme activity in plasma and aorta. Results: CCX improved in a dose-dependent manner acetylcholine relaxation in the aorta of the treated SHR. Plasma malonildialdehyde decreased in the CCX treated SHR. The effects mentioned before were not observed in the SHR after the withdrawal of CCX. CCX treatment did not modify aorta angiotensin converting enzyme activity, but the activity of this enzyme increased in the plasma of the SHR treated with the highest dose of CCX. Conclusion: The antihypertensive effect of CCX in SHR is mainly mediated by an improvement of endothelial function and by a reduction of oxidative stress.