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Assessment of acrosomal integrity of vervet monkey spermatozoa after cryopreservation

Cryopreservation of Vervet monkey semen caused a highly significant reduction in the percentage of normal acrosomes and a highly significant increase in the percentage of mildly damaged, severely damaged, and lost acrosomes. This was demonstrated by staining ten post‐thaw semen samples, which enabled a visualization of the acrosomal damage. The incidence of post‐thaw intact acrosomes in this cryopreservation method was found to be similar to that reported for man and cynomolgus monkey semen.