{'fraction_non_alphanumeric': 0.03510945890128046, 'fraction_numerical': 0.024783147459727387, 'mean_word_length': 5.053, 'pattern_counts': {'":': 0, '<': 5, '<?xml version=': 0, '>': 1, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The impact of visceral fat and levels of vitamin D on coronary artery calcification

SUMMARY OBJECTIVE: To evaluated calcification of the coronary arteries and its association with visceral fat and 25-hydroxyvitamin D (25(OH)D) serum levels. METHODS: A cross sectional study involving 140 individuals without any previous diagnosis of cardiovascular disease. A biochemical analysis of vitamin D serum levels was carried out, as well as computed tomography to measure coronary artery calcium score and visceral adipose tissue. RESULTS: The mean age of the individuals was 55.9 ( ± 12.4). Coronary artery calcium was observed in 40.7% of the population. Vitamin D presented median serum levels of 30.4 ng/ml (IQ24.5–39.1), with 14,1 and 33.7% of the individuals presenting deficiency and insufficiency, respectively. In the univariate analysis, the calcium score was more prevalent in aged patients (p<0.01), in hypertensive individuals (p<0.01), in diabetics (p=0.02), and in those with a higher concentration of VAT (p=0.02). In the adjusted analysis, it was found that the highest concentration of VAT (OR: 4.0; 95%CI 1.4–11.7), hypertension (OR: 4.8; 95%CI 1.5–15.3), and age (OR: 10.4; 95%CI 3.9–27.6) were predictors of subclinical atherosclerosis, regardless of body mass index, diabetes, and 25OHD. CONCLUSIONS: Excess visceral fat was associated with subclinical atherosclerosis, regardless of other risk factors for cardiovascular disease. Serum levels of 25OHD were not associated with CAD in its early stages.


INTRODUCTION
Cardiovascular diseases (CVD) are highly prevalent worldwide. They are an important cause of morbidity and the main cause of mortality in Brasil and the world 1 . CVD have insidiously develop over decades, and their first signs can be fatal or highly limiting. Thus, identifying and modifying the pathological process in the initial subclinical stages of CVD can be clinically challenging 2 .
Calcification of coronary arteries (CCA), also known as subclinical atherosclerosis, is currently characterized as a dynamic process of biomineralization, complexly regulated and closely related to the degree of inflammatory activity 3,4 . Several bone remodeling regulators, such as osteocalcin, hydroxyapatite crystals, osteopontin, bone morphogenetic protein 2, osteoprotegerin, leptin, and oxidized lipids, and factors related to calcium sensor have been described in calcified atherosclerotic lesions 4 .
Vitamin D plays an important role in regulating mineral and bone metabolism 5 . A recent meta-analysis correlated low vitamin D levels with increased cardiovascular risk 5 , suggesting that hypovitaminosis D may be an undervalued risk factor for CVD. Evidence accumulated in recent decades indicates that the beneficial role of vitamin D for cardiovascular health involves genomic mechanisms responsible for non-classical effects of vitamin D and are mainly mediated by its active forms, 1α 25-hydroxyvitamin D (1α,25(OH)D) and 1.25-hydroxyvitamin D (1.25(OH)D), interacting with its intracellular receptor 6,7 .
Excess body fat is related to many diseases, including CVD and cerebrovascular disease. However, evidence from the literature suggests that, compared to total body fat, fat deposited in the abdominal region is a better predictor of high coronary risk 8,9 . Furthermore, visceral adipose tissue (VAT) has been shown to predict differentiated cardiometabolic risk 10 .
Thus, this study aimed to evaluate the association between CCA, measured by the calcium score, and serum 25(OH)D and VAT levels.

METHODS
The study protocol was approved by the Ethics and Research Committee on Human Beings of Universidade de Pernambuco. Patients aged ≥35 years without a prior history of heart disease who were consulted in the outpatient department between June 2014 and October 2015 were included. Individuals with hepatitis and/or splenomegaly, ascites, recent abdominal surgery, chronic renal failure, and carriers of hypothyroidism or hyperthyroidism and pregnant women were excluded. Informed consent was obtained from all participants.
CCA (the right coronary aorta, left coronary trunk, anterior descending artery and its branches, and circumflex artery and its branches) was classified as absent (0) or present (>0) and evaluated by computed tomography (CT) with multiple detectors. Lesions with a minimum density of 130 Hounsfield units (HU) and a minimum area of 0.5 mm 2 were detected.
VAT levels were evaluated using non-contrast abdominal CT (Philips Brilliance 10 Slice CT Scanner, VMI Indústria e Comércio Ltda, Lagoa Santa, MG, Brasil) by a single observer trained in the study protocol after a 4-hour complete fasting. Density values between -50 and -250 HU were used to identify adipose tissue. A high volume of visceral tissue and subcutaneous adipose tissue (SAT) was considered when the value was above the 75 th percentile of the distribution (VAT ≥316.0 cm 3 and SAT ≥536.2 cm 3 ).
Among the anthropometric variables, body mass index (BMI) and abdominal circumference (AC) were considered. BMI was classified according to the classification proposed by the World Health Organization, and the measurement of AC was obtained at the midpoint between the last rib and the iliac crest.
Data were analyzed using the Statistical Package for Social Sciences, version 22.0. Associations between categorical variables were evaluated by the χ 2 test. Variables that reached statistical significance (p≤0.20) in the univariate analysis were subsequently included in the logistic regression model. The significance level was set at p<0.05 for all statistical analyses.

RESULTS
A total of 161 patients were eligible for the study. However, 6 patients refused to participate and 15 did not undergo all the proposed tests. Thus, a total of 140 patients were finally included.
The mean patient age was 55.9±12.36 years, and 72.2% patients were women, 72% patients were non-white, and 92.3% patients had low socioeconomic status. Furthermore, 28.9% patients had diabetes and 63.4% had hypertension ( Table 1).
The presence of CCA was observed in 40.7% of the studied population. The median serum vitamin D levels were 30.4 (IQR 24.5-39.1) ng/mL; 12.9% of patients had deficient levels and 33.7% had insufficient levels. The median visceral compartment volume was 277.38±95.75 cm 3 (Figure 1).
In univariate analysis, the prevalence of CCA was higher in aged (p<0.01), hypertensive (p<0.01), and diabetic (p=0.02) individuals and in those with a higher concentration of VAT

DISCUSSION
The detection of CCA, also known as subclinical atherosclerosis, has been proposed as a strategy to improve the identification of individuals at high risk of cardiovascular events, especially those in which traditional screening tools can underestimate the risk. This allows the implementation of more effective prevention measures.
The prevalence of CCA in this study (40.7%) was similar to that reported in the Third Generation of the Framingham Heart Study cohort study (42.5%) 10 . Our results reinforce the findings of a high prevalence of CCA, even in populations without a previous history of CCA, which indicates that a high number of individuals are at risk for cardiovascular events. Based on the results of several studies, the main cardiological guidelines recommend the use of CCA for risk stratification in asymptomatic patients.
As described in previous studies, vascular calcification is more prevalent in aged, hypertensive, and diabetic individuals 11 . This is also in line with the results reported by Julie Anne Hof et al. 12 in a population-based study including 35,500 participants. Given that the atherosclerotic process usually occurs over several years, aging is one of the most consistent and robust factors involved in the incidence of CVD.
Results of univariate and multivariate analyses showed an increased prevalence of CCA among hypertensive individuals, consistent with the results of previous studies 13,14 . Although the inter-relationship between hypertension, coronary atherosclerosis, and calcification is not fully understood, some mechanisms, such as the induction of trauma to the arterial wall, have been proposed. Trauma to the arterial wall has been suggested to induce the expression of osteopontin, a protein involved in mineralization.
The prevalence of vitamin D deficiency/insufficiency in our study (33.0%) was lower than that reported in other studies in the Brazilian population-Bandeira et al. 15 reported a prevalence of 66.7% and Maeda et al. 16 reported a prevalence of 73%. Differences in the studied population could explain the lower prevalence of vitamin D deficiency/insufficiency in our study. Both abovementioned studies 15,16 included individuals with a higher mean age (69.4 and 79.6 years, respectively) than those in the present one (55.9 years) and some institutionalized patients; hence, their exposure to sunlight may have been limited. In addition, aging may cause decreased intestinal absorption and impaired hydroxylation in the liver and kidneys. A relationship between vitamin D levels and CVD has been proposed, but it has not been well established. Although we have not evidenced this association in our findings, some authors have shown that low serum 25(OH)D levels were related to increased carotid intima-media thickness 17 and CCA in adults without symptoms of CVD 18 . An in vitro study showed that vitamin D inhibited the proliferation of vascular smooth muscle cells 19 . Another study showed that low serum vitamin D levels were associated with activation of the renin-angiotensin system 20 .
Our study cohort included a high percentage of patients who were overweight (74.1%), those with abdominal obesity (86.6%), and those with a higher mean VAT. Only excess VAT was associated with CCA in univariate and multivariate analyses, increasing the chance of presenting CCA by four times compared to individuals with a lower VRT concentration.
Corroborating our findings, the NeoStudy 21 found an association between subclinical atherosclerosis, measured by the thickness of the carotid artery intima media, and vertical auto profile cholesterol test results in men and women aged 45-65 years. Similar findings were obtained in the Multi-Detector Computed Tomgraphy study, which included individuals with a mean of 50 years; the study reported a lower risk of subclinical atherosclerosis in patients with lower values of visceral adiposity 22 .
The deleterious effect of increased visceral fat concentration appears to be linked to the release of free fatty acids into liver circulation, which stimulates the release of apolipoprotein B, thus promoting insulin resistance and leading to increased plasma glucose levels, with consequent endothelial dysfunction 23 . Other mechanisms have also been proposed, such as increased release of inflammatory cytokines, adiponectin, interleukin 6, and inhibitor of activation of plasminogen 1 by VAT, which appear to be involved in the genesis of the atherogenic process.
This finding reinforces the importance of assessing the distribution of body fat rather than global obesity while tracking the risk of CVD. This is in line with the observations reported by See et al., who provided evidence of a correlation of CCA with VAT, but not with BMI 24 .
Our study has some limitations. First, the study had a cross-sectional design. Second, the relatively small sample size and the larger proportion of women may limit the generalization of results. However, the use of CT, considered the "gold standard" for measuring the visceral compartment, is an important strength of our study.

CONCLUSIONS
Excess visceral fat is associated with subclinical atherosclerosis, independent of other risk factors of CVD. Here, serum 25(OH) D levels were not associated with CCA in its early stages.
More research is needed to achieve more definitive conclusions on the association between these parameters and CCA. However, it is important in clinical practice to adopt strategies for the analysis of the intra-abdominal fat composition as a method of CCA risk screening.