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The preparation of Ca2+–selective electrodes

Ion–selective electrode (ISE) is a useful tool in the direct determination of ionic species in complex samples. This study relates to the manufacture of Ca 2+ –selective electrode with single–barrelled microelectrodes and an internal filling solution buffered for pCa (pCa= –lg[Ca 2+ ]) of 6. It is shown that an improved lower detection limit of 10 –6 M is obtained in Ca 2+ buffer solution and the changes in electromotive force between two solutions of 10–fold change in Ca 2+ concentration are close to 30 mV/decade.