{'fraction_non_alphanumeric': 0.024149286498353458, 'fraction_numerical': 0.0, 'mean_word_length': 6.6, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Upregulation of antithrombotic ectonucleotidases by aspirin in human endothelial cells in‐vitro

Abstract— Ecto ATP‐diphosphohydrolase (apyrase) activity of human endothelial cells following aspirin treatment has been studied in‐vitro. It was shown by HPLC analysis of supernatant samples that pre‐incubation of the cultures with aspirin resulted in a significantly increased turnover of supplemented ATP into its degradation products (ADP and AMP). Enhanced expression of ectoenzyme after aspirin treatment could be observed as demonstrated by immunofluorescence‐staining with monoclonal anti‐apyrase antibodies. This suggests enhancement of endothelial ATP‐diphosphohydrolase activity induced by aspirin. The present data obtained in human vascular cells in‐vitro are in line with results from previous animal studies in‐vivo, suggesting a novel cyclo‐oxygenase‐independent antithrombotic activity of aspirin.