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Methods for mass screening of vitamin A deficiency.

Vitamin A deficiency is a major cause of blindness in preschool and school aged children in developing countries. Common illnesses including measles, lower respiratory tract infections and diarrhea are commonly associated with vitamin A deficiency. While clinically evident vitamin A deficiency in patients with protein energy malnutrition or in sick children gets proper attention, it is likely that many cases remain undetected. It is important to diagnose cases of marginal vitamin A deficiency as early as possible so that preventive measures can be taken. Several methods are available for diagnosis of vitamin A deficiency. The most commonly used approaches are assessment of dietary vitamin A, biochemical assessment of serum vitamin A, ophthalmological examination for clinical signs of vitamin A deficiency, Rose Bengal stain test (RBST) for early conjunctival xerosis, and conjunctival impression cytology (CIC) for preclinical vitamin A deficiency.