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Change in energy substrate utilization in the heart of diabetic rats and its effect on high energy phosphate compound

Isolated hearts of type 2 diabetic rats were perfused with K H buffer containing glucose and 3 H labeled palmitate by a Langendorff perfusion apparatus. The results revealed that glucose uptake by hearts of diabetic rats was significantly depressed, glucose oxidation was decreased and palmitate oxidation was increased. The myocardial ATP content declined gradiently. Change in energy substrate utilization seems to be a significant factor contributing to cardiomyopathy and rendered diabetes more vulnerable to ischemic injury.