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Cloning and expression of fragment of the human ALEX2 cDNA

AIM: To clone fragment of the human ALEX2 gene, to construct the recombinant vector and to induce its expression. METHODS: The fragment of the human ALEX2 gene was amplified by RT PCR from human placenta brain tissue and cloned into PGEM T easy vector. After sequencing, it was subcloned into prokaryotic expression vector PGEX 4T 3. After induced by IPTG for 4 h, the expression of recombinant M r 417 000 ALEX2 protein was confirmed by SDS PAGE. RESULTS: The fragment of the human ALEX2 gene was cloned. The DNA sequence was identical with that published by GenBank. The recombinant plasmid PGEX ALEX2 was constructed. The expressed fusion protein was about 15.5% of total bacterial protein by gel thin layer chromatography scanning. CONCLUSION: The fragment of the human ALEX2 gene and prokaryotic expression products are obtained. It may be of great significance to the preparation of some monoclonal antibody against human ALEX2 and the study on the function of human ALEX2.