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Regulatory effect of JAK/STAT signaling pathway on enterovirus 71 infection

Objective To investigate the role of the Janus kinase / signal transducers and activators of transcription( JAK / STAT) signaling pathway in infection of EV71. Methods The mRNA levels of 4 interferon stimulated factors( ISGs) including ISG15,ISG56,OAS1,MXA in human rhabdosarcoma( RD) cells and human monocyte-macrophagic leukemia cell line( THP-1) were detected by RTPCR. The effect of IFN-α2b( 1 000 U / m L) on the infection and replication of EV71 were also observed,and the total and phosphorylated level of STAT1 were analyzed by western blot. Results The expression of MXA mRNA decreased in EV71-infected RD cells,but no phosphorylated STAT1 was found. The expression of MXA,OAS1 and ISG56 mRNA and phosphorylated STAT1 level were increased in EV71-infected THP-1cells. Compared with IFN group,the levels of ISG15,ISG56,OAS1 and MXA mRNA,as well as phosporylated STAT1 were decreased in the EV71-infected RD cells treated with IFN-α2b. The expressions of 4 ISGs and phosphorylated STAT1 level significantly were elevated in THP-1cells. Conclusion Infection of EV71 could block JAK / STAT signaling pathway in RD cells and the opposite results were observed in THP-1 cells. These results suggest that THP-1 cells might play a role in innate immune response in the patients infected with EV71.