{'fraction_non_alphanumeric': 0.0328125, 'fraction_numerical': 0.0265625, 'mean_word_length': 5.047169811320755, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Chemical mechanism of 6-phosphogluconate dehydrogenase via kinetic studies and site­ directed mutagenesis



6-Phosphogluconate dehydrogenase catal)jzes the metal ion-independent oxidative decarboxylation of 6- phosphogluconate (6PG). The enzyme catalyzes a reaction in which 6PG is first oxidized at the 3-position, then decarboxylated to the 1,2-enediol of Ru5P , and then tautomerized to give the Ru5P ketone product. In the reaction, Kl83 acts as a general base, general acid, shuttling a proton between the 3-hydroxyl and itself, and E190 acts as a general acid to protonate C1 of the enediol intermediate in the tautomerization step.
