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Quantitative morphological analysis in a SEM‐microcomputer system—I. Quantitative shape analysis of single objects

The combination of a scanning electron microscope and a microcomputer can be used to carry out automatic, quantitative, morphological analysis of a wide range of samples. A system implemented around a commercially available 8‐bit computer and interface hardware is described. Computerized analysis of shape is most accurately carried out by the use of numerically determined Fourier transforms of the high contrast bright field transmitted electron image; however, Walsh functions offer very significantly faster processing times on smaller computer systems.