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Induction of MiR‐17‐3p and MiR‐160a by TNFα and LPS

MicroRNAs (miRNAs), small non‐coding molecules, regulate gene expression in response to stimuli. Lipopolysaccharide (LPS) was reported to induce the expression of miR‐146 and miR‐155 in HL‐60. The effects of LPS and the related stimulus, tumour necrosis factor alpha (TNFα), on miRNA expression required to be further studied. Using T7‐oligo ligation assay (OLA)‐based miRNA array, we profiled the expression of 132 miRNAs and identified a number of TNFα‐regulated miRNAs in HeLa cells, including miR‐17‐3p and miR‐106a. TNFα induction of miR‐17‐3p and miR‐106a was verified by Northern blot analysis with RNU48 normalization. Northern blot analysis also showed that LPS was able to induce the expression of both miR‐17‐3p and miR‐106a in HeLa cells. Furthermore, both array assay and Northern blot analysis showed that the expression levels of miR‐146 and miR‐155 were either low or undetectable in HeLa cells and TNFα‐ and LPS‐mediated induction of these two miRNAs was not found. Luciferase reporter analysis confirmed the induction of miR‐17‐3p and miR‐106a in response to TNFα and LPS treatment in HeLa cells. These results suggested that the expression of miR‐17‐3p and miR‐106a is regulated by TNFα and LPS in HeLa cells. Copyright © 2011 John Wiley & Sons, Ltd.