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Effects of Silencing TT8 and HB12 on in vitro Nutrients Degradation and VFA Production in Relation to Molecular Structures of Alfalfa (Medicago sativa).

BACKGROUND
Transparent Testa8 (TT8) and Homeobox12 (HB12) are two transcriptional factors in plant phenylpropanoid pathway and were reported to be positively related to lignin content. Therefore, alfalfa with silenced TT8 (TT8i) and HB12 (HB12i) were generated via RNAi technique. Although lignin was found high in HB12i, such gene silencing of alfalfa resulted in nutrient profiles that might suitable for grazing condition. To extend the nutritional evaluation of transformed alfalfa, ground samples of 11 HB12i, 5 TT8i and 4 wild type (WT) were incubated in rumen fluid: buffer solution for 0, 2, 4, 8, 12, 24 and 48 h at 39 °C. Dry matter (DM) and neutral detergent fiber (NDF) degradations at each time point, and volatile fatty acids (VFA) production at 4, 12, 24 and 48 h were analyzed, as well as degradational and productional kinetics. Moreover, correlations and regressions of nutritive profiles with attenuated total reflection- Fourier transform infrared (ATR-FTIR) spectral parameters were determined.


RESULTS
Both transformed genotypes had lower DM degradation and HB12i had lower VFA production compared with WT. In addition, structural carbohydrate (STC) parameters were found negatively correlated with DM degradation and VFA production. Also, kinetics of DM degradation and VFA production were predicted from spectral parameters with good estimation power.


CONCLUSION
Silencing of HB12 and TT8 affected fermentation characteristics of alfalfa and some fermentation characteristics were predictable from spectral parameters with ATR-FTIR spectroscopy. This article is protected by copyright. All rights reserved.