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Antibacterial Activity of Ozonized Sunflower Oil, Oleozón, Against Staphylococcus aureus and Staphylococcus epidermidis.

Abstract Ozonized sunflower oil, Oleozón, has shown antimicrobial effects against several bacterial species. The purpose of this paper was to study in vitro activity of ozonized sunflower oil, Oleozón, on Staphylococcus aureus and Staphylococcus epidermidis, methicillin susceptible and methicillin resistant strains. Minimal Inhibitory Concentrations (MICs) and Minimal Bactericidal Concentrations (MBCs) were determined based on National Committee for Clinical Laboratory Standards (NCCLS) guidelines. Meanwhile, kinetic studies were performed. Oleozón was effective against all strains, MIC90 was 9.5 mg/mL and MBC90 was 356 mg/mL. First order inactivation kinetics with respect to bacterial concentrations were found. The longest time necessary to obtain the total inactivation was 60 minutes for methicillin susceptible strains and 180 minutes for methicillin resistant strains.