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Clinical value of TRFIA and ELISA in the detection of five indicators of hepatitis B virus

Objective 
To investigate the clinical value of time-resolved fluoroisnmunoassay (TRFIA) and enzyme-linked immunosorbent assay (ELISA) in the detection of five indicators of hepatitis B virus. 
 
 
Methods 
A total of 180 patients with hepatitis B were selected. And 2 samples of fasting venous blood of 2 ml were taken from each patient and then were detected by TRFIA and ELISA repectively. The detection results of five indicotors of hepatitis B virus were compared. 
 
 
Results 
The positive rate of hepatitis B e antibody (HBeAb) and hepatitis B core antibody (HBcAb) detected by TRFIA was 52.78% (95/180) and 70.56% (127/180) respectively, the positive rate of HBeAb and HBcAb detected by ELISA was 42.22% (76/180) and 59.44% (107/180), respectively, and there was a statistical difference in positive rate of HBeAb and HBcAb between the two groups (P 0.05). 
 
 
Conclusions 
Both TRFIA and ELISA can be used in the detection of five indicators of hepatitis B virus. Compared with ELISA, the positive detection rate of TRFIA is higher, which is worthy of clinical application and promotion. 
 
 
Key words: 
Time resolved fluoroisnmunoassay; Five indicators of hepatitis B virus; Enzyme-linked immunosorbent assay