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PHOTOREVERSIBLE Ca2+‐DEPENDENT AGGREGATION OF PURIFIED PHYTOCHROME FROM ETIOLATED PEA AND RYE SEEDLINGS

The aggregation of phytochrome purified from etiolated pea (Pisum satirum cv. Alaska) and rye (Secale cereale cv. Cougar) tissues was investigated by centrifugation and turbidimetry. Purified pea phytochrome (A669/A280= 0.88), if irradiated with red light, became precipitable in the presence of CaCl2. The precipitation upon red‐light irradiation was optimal at a Ca2‐ or Mg2+ concentration of 10–20 mM, was greater at increased phytochrome concentration or lower pH values, and was inhibited by 0.1 M KG. The precipitated phytochrome slowly became soluble after far‐red light exposure.