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Anthraquinones with Antiplasmodial Activity from the Roots of Rennellia elliptica Korth. (Rubiaceae)

Dichloromethane root extract of Rennellia elliptica Korth. showed strong inhibition of Plasmodium falciparum growth in vitro with an IC50 value of 4.04 µg/mL. A phytochemical study of the dichloromethane root extract has led to the isolation and characterization of a new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone (1), and ten known anthraquinones: 1-hydroxy-2-methoxy-6-methyl-9,10-anthraquinone (2), nordamnacanthal (3), 2-formyl-3-hydroxy-9,10-anthraquinone (4), damnacanthal (5), lucidin-ω-methyl ether (6), 3-hydroxy-2-methyl-9,10-anthraquinone (7), rubiadin (8), 3-hydroxy-2-methoxy-6-methyl-9,10-anthraquinone (9), rubiadin-1-methyl ether (10) and 3-hydroxy-2-hydroxymethyl-9,10-anthraquinone (11). Structural elucidation of all compounds was accomplished by modern spectroscopic methods, notably 1D and 2D NMR, IR, UV and HREIMS. The new anthraquinone 1, 2-formyl-3-hydroxy-9,10-anthraquinone (4) and 3-hydroxy-2-methyl-9,10-anthraquinone (7) possess strong antiplasmodial activity, with IC50 values of 1.10, 0.63 and 0.34 µM, respectively.


Introduction
Rennellia elliptica Korth. (Rubiaceae) is a tropical shrub locally known in Malaysia as Segemuk or Mengkudu Rimba. This plant is widely distributed, along riverbanks or lowland forest, in Peninsular Malaysia, Sumatra and Borneo [1]. Decoctions of the root are taken by the locals for various purposes, including as an aphrodisiac, for body aches and as a post natal tonic [2]. Not much has been reported on the chemical constituents or biological properties of this plant. A preliminary study by Yusoff et al. on the roots of the plant reported one anthraquinone compound [3]. Our screening of some Malaysian plant extracts for antiplasmodial activity showed that the dichloromethane roots extract of R. elliptica is a potential source of antiplasmodial compounds (IC 50 = 4.04 µg/mL). This paper reports the isolation, structure elucidation and antiplasmodial activity of a series of anthraquinone compounds, including a new one, from the root of R. elliptica.

Results and Discussion
Eleven anthraquinones ( Figure 1) were isolated from roots of R. elliptica after extensive chromatographic separation and were characterized by analysis of spectroscopic data and by comparison with literature values [4][5][6][7][8][9][10][11][12][13]. The structures of compounds 4 and 6 were also confirmed using x-ray crystallography [14,15]. The compound 1-hydroxy-2-methoxy-6-methyl-9,10anthraquinone (2) was described by Mittie and Biswas in 1928 [16]. However, since then there have been no further reports on the natural occurrence of this compound and there is no spectroscopic data available in the literature for comparison. Hence, we elucidated the structure by careful analysis of MS, IR, UV and NMR data, followed by confirmation through x-ray crystallography [17]. In this paper we include the full spectroscopic data for this compound.
The new compound, 1,2-dimethoxy-6-methyl-9,10-anthraquinone (1) was obtained as bright yellow amorphous solid. The HREIMS displayed a [M + H] + peak at 283.0968 [calc 283.3067] suggesting a molecular formula of C 17 H 14 O 4 . The absorption maxima in the UV spectrum were observed at 373, 341 and 257 nm, indicative of an anthraquinone moiety [18]. The IR spectrum did not show presence of chelated carbonyl and hydroxyl groups. The sp 2 C-H stretch for the aromatic ring was observed at 3,081 cm -1 . With the exception of the sharp singlet in the downfield region for the hydrogen-bonded hydroxyl group, the 1 H-NMR spectrum resembles that of compound 2, suggesting a similar substitution pattern. Splitting pattern of the five aromatic proton signals suggested substitutions on both rings. Two overlapping doublets centered at δ H 8.17 are due to H-8 (d, J = 7.8 Hz) and H-4 (d, J = 8.7 Hz), the peri-hydrogens. A doublet at δ H 7.28 (J = 8.7 Hz) is due to H-3, meanwhile H-7 gave another doublet of doublet at δ H 7.58 (J o = 7.8 Hz, J m = 1.7 Hz). These assignments were confirmed by their respective correlations in the COSY spectrum. H-5 resonated as a singlet at 8.06 ppm. In addition, two sharp singlets at δ H 2.53 (3H, s) and 4.02 (6H, s) due to a methyl and two methoxy groups, respectively, were also observed.
The location of the methoxy groups were established at C-1 and C-2 of ring C based on its NOE correlation with H-3. Thus, the only possible location for the methyl substituent is at C-6. This assignment was confirmed through NOE correlations of the methyl group with H-5 and H-7. These NOE correlations are illustrated in Figure 2. The placement of methyl group at C-6 was further confirmed by HMBC experiment which showed a 3 J correlation with H-7. The methine carbons (C-3, C-4, C-5, C-7 and C-8) were assigned through HMQC correlations while the quaternary carbons (C-1, C-2, C-6, C-11, C-12, C-13 and C-14) were assigned based on careful analysis of HMBC spectrum. Both carbonyl carbons in this compound resonated very closely to each other with only 0.01 ppm difference at δ C 182.70 and 182.71, which further confirmed the unchelated nature of the carbonyls.
Close inspection of all spectroscopic data confirmed that compound 1 is 1,2-dimethoxy-6-methyl-9,10-anthraquinone, the 1-methyl ether of compound 2. Complete 1 H and 13 C data of compounds 1 and 2 are presented in Table 1.  Anthraquinones isolated from the root of R. elliptica exhibited the typical substitution pattern of Rubia type anthraquinones, with most of them substituted only on ring C [19]. The anthraquinones from Morinda and Prismatomeris spp. in the same family also exhibited a similar substitution pattern [4][5][6][7][8][9]12,13,[20][21][22]. Genus Rennellia is classified in the same tribe as Morinda and Prismatomeris [23], so the anthraquinones may be produced through similar biosynthetic pathways, which explains the similarity in the substitution patterns [19]. However, anthraquinones 1, 2 and 9 which are substituted on both rings have a methyl substitution at C-6, differing from the anthraquinones of Prismatomeris and Morinda which are typically hydroxyl or methoxy substituted at C-6.
We screened the anthraquinones isolated from the roots of R. elliptica for antiplasmodial activity based on the promising screening results of dichloromethane crude extract (IC 50 = 4.04 µg/mL). The in vitro antiplasmodial activity of anthraquinones isolated from R. elliptica against a chloroquine sensitive strain of P. falciparum (3D7) is shown in Table 2. Compound 7 displayed the strongest inhibition activity, with an IC 50 value of 0.34 µM, followed by compound 4 with an IC 50 value of 0.63 µM. Sittie et al. established that an aldehyde group at C-2 and a phenolic hydroxy group at C-3 on the anthraquinone skeleton enhance the activity of anthraquinones against the growth of P. falciparum [24]. Our results show that methyl group at C-2 together with phenolic hydroxy group at C-3 as in compound 7 also gave significant activity. It should also be noted that both compounds 4 and 7 do not possess hydroxyl substituents at the peri positions. The new anthraquinone 1 also exhibited strong inhibition, with an IC 50 value of 1.1 µM. Interestingly, anthraquinone 2, which structurally differs only at C-1 (hydroxyl substituent instead of methoxy substituent) did not show any significant activity. The position of substituents on anthraquinone skeleton clearly influences the antiplasmodial activity, which warrants further investigation. We are currently synthesizing anthraquinone derivatives with various substitution patterns for the purpose of establishing structure-activity relationships.

Instrumentation
1 H-and 13 C-NMR spectra were run on a Bruker 300 Ultrashield NMR (Switzerland) at 300 and 75.5 MHz, respectively, using CDCl 3 or acetone -d6 (Merck) as solvent. Chemical shifts are reported in ppm and δ scale with the coupling constants given in Hz. Melting points were determined using a Hinotek X-4 (China) melting point apparatus equipped with a microscope and are uncorrected. HREIMS spectra were obtained on a Thermo Finnigan Automass Multi (Shimadzu, Japan). IR spectra were obtained using Perkin-Elmer 1600 series FTIR spectrometer (USA) using KBr pellets. UV spectra were recorded on Shimadzu UV-160A spectrometer (Japan) in absolute ethanol (Scharlau) and alkaline ethanol.

Chemicals and Reagents
Column chromatography was carried out using silica gel (silica gel 60, 230-400 mesh, Merck, Germany). n-Hexane, dichloromethane and methanol for column chromatography were freshly distilled from industrial grade solvents. The fractions collected were monitored using analytical TLC (Merck, Germany), pre-coated with silica gel 60 F 254 of 0.25 mm thickness and visualized under UV light at 245 nm and 356 nm. PTLC was carried out using pre-coated plate with PSC-Fertigplatten Kieselgel 60 F 254 (1.0 mm thickness, 20 × 20 cm) purchased from Merck. Plasmodium falciparum (3D7) strain was used for in vitro antiplamodial tests. All chemicals used for determination of antiplasmodial activity were purchased from Sigma-Aldrich, unless otherwise stated.

Plant Material
The roots of R. elliptica were collected from Kuala Keniam, National Park, Pahang, Malaysia at altitude 165 m above sea level and were identified by Dr Shamsul Khamis, Universiti Putra Malaysia.
The voucher specimens (SK1512/08) were deposited at Herbarium of Institute of Bioscience, Universiti Putra Malaysia and Universiti Teknologi MARA.

Determination of Antiplasmodial Activity
The antiplasmodial activity of dichloromethane extract and the isolated compounds were determined by methods as previously described [25]. The samples were dissolved in DMSO and kept at -20 ºC until used. The malarial parasite P. falciparum (3D7) clone was propagated in a 24-well culture plate in the presence of 10, 1, 0.1, 0.01 and 0.001 µg/mL range of concentrations of each compound. Chloroquine diphosphate was used as positive control. The growth of the parasite was monitored by making a blood smear fixed with MeOH and stained with Geimsa (Merck). The antiplasmodial activity of each compound was expressed as an IC 50 value, defined as the concentration of the compound causing 50% inhibition of parasite growth relative to an untreated control.

Conclusions
A phytochemical study on dried roots of R. elliptica afforded a new anthraquinone, 1,2-dimethoxy-6-methyl-9,10-anthraquinone (1), together with ten known anthraquinones 2-11. Several anthraquinones strongly inhibited in vitro growth of a chloroquine sensitive strain of Plasmodium falciparum (3D7), with the strongest inhibition shown by compound 7. The antiplasmodial data suggested that the roots extract of R. elliptica is a potential source of new antiplasmodial agents.