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Cloning of the autophagy‐related gene Beclin‐1 of the marine teleost Paralichthys olivaceus and its possible implication in viral replication

Autophagy is essential for the maintenance of cellular homeostasis and for the orchestration of an efficient cellular response to stress. Beclin‐1, one of the central regulators of autophagy, participates in development and aging, and in protection against certain types of cancer, neurodegeneration and pathogen invasion. In the present study, we isolated the full‐length cDNA of PoBeclin‐1 from the marine teleost olive flounder Paralichthys olivaceus by screening of gill cDNA library and rapid amplification of cDNA ends (RACE). The PoBeclin‐1 cDNA is 2,179bp and encodes the polypeptides with 447 amino acids containing conserved Bcl‐2 binding and BH3 domains. PoBeclin‐1 transcripts were detected from early larva stage and ubiquitously in healthy flounder. The expression of PoBeclin‐1 mRNA was induced in the kidney and spleen challenged by viral hemorrhagic septicemia virus (VHSV), an economically important pathogen for aquaculture of flounder. Over‐expression of PoBeclin‐1 increased the expression of VHSV G protein and viral replication in HINAE cells. These results suggest that a potential role for PoBeclin‐1 in viral replication in flounder.