{'fraction_non_alphanumeric': 0.02442635085122132, 'fraction_numerical': 0.008882309400444115, 'mean_word_length': 4.878260869565217, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Self peptide/MHC class I complexes have a negligible effect on the response of some CD8+ T cells to foreign antigen

MHC molecules loaded with self peptides do not trigger a T cell immune response but may deliver signals important for peripheral T cell survival and function. It is unclear if self peptide/MHCcomplexes on APC in addition can influence the T cell response to co‐presented foreign ligands. To address this question, TAP‐sufficient and TAP‐deficient cells were loaded with ovalbumin peptide (pOVA) to generate APC that present pOVA/H‐2Kb complexes in the context of high or low levels of self peptide‐loaded MHC class I, respectively. The two cell types were then used to stimulatedifferent CD8+ T cells specific for ovalbumin while the number of presented pOVA/H‐2Kb complexes was independently assessed by staining with 25‐D1, an antibody against pOVA/H‐2Kb. In each case, T cell activation was independent of TAP expression by the APC and depended exclusively on the amount of 25‐D1 staining. We conclude that the number of pOVA/Kb complexes and not their frequency relative to self peptide/MHC complexes determines the response of those T cells tested here. These results imply that the repertoire of self peptide/MHC class I complexes presented by APC has a negligible effect on the response of some CD8+ T cells to foreign ligands.