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Alterations of central nervous system protein synthesis in response to spinal cord hemisection

This investigation explores the central nervous system protein‐synthetic responses to hemisection of the spinal cord. Adult male rats hemisected at mid T2 were given intravenous injections of 14C‐leucine at 0, 7, 30, or 70 days postoperative. Four hours later the T1, T2, T3, and C6 segments of the spinal cord and right and left samples of somatomotor and occipital cortex were removed, weighed, and frozen. These samples were later homogenized ad subjected to trichloroacetic acid (TCA) separations. Protein‐synthetic activity was expressed as the ratio of the specific activity of the TCA precipitable proteins to the specific activity of the TCA free amino acid pool.