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Further Characterization and Species Specificity of Leukocytic Endogenous Mediator (LEM)1

Summary A degree of cross species susceptibility was observed on the effects of leukocytic endogenous mediator (LEM) on serum zinc depression and amino acid flux. LEM was shown to be protein in nature, and relatively stable under a wider range of pH and conditions of storage. Although LEM shares several chemical and physical characteristics with endogenous pyrogen (EP), some differences between the chemical properties and species specificity of these two mediators were noted.