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ASSOCIATIONS BETWEEN BLOOD CONCENTRATIONS OF CYTOTOXIC CD8+ CELLS 
AND LYMPHOCYTE APOPTOSIS IN HEALTHY HUMANS

Introduction: Cytotoxic T-lymphocytes play an important role in a specific immune response via a lytic effect in relation to abnormal cells. The number of these cells greatly increases sin pathological states. In addition, an increased cytotoxic activity is a characteristic of the immune response of people living in the Far NorthTherefore, it is important to understand what function cytotoxic T-lymphocytes predominantly perform in healthy people in the North - cytolytic or suppressor? Aim: To assess associations between CD8+ concentration and immune response and apoptotic deaths of the lymphocytes in healthy individuals. Methods: Ninety-three healthy adult residents of the Russian North comprised the sample. Apoptotic lymphocyte death was studied by flow cytometry. FITC-annexine-V and propidium iodide labelled cells were detected. Concentrations of cytokines and apoptosis mediators were assessed by a solid-phase enzyme immunoassay. Neutrophiles, monocytes and phagocytic activity of neutrophils were studied in blood smears stained by Romanowsky's - Giemsa. The level of phenotypic activity of lymphocytes was assessed by double peroxidase labeling using monoclonal antibodies. The data were presented using means, standard deviations, medians, the 1 st and the 3 rd quartiles. All study participants were divided into two groups: with normal- (0.2-0,4 × 10 9 kl/l) and elevated (more 0,6 × 10 9 kl/l) blood cytotoxic lymphocyte levels. The groups were similar in terms of by age- and gender distribution. Continuous variables were analyzed using Mann-Whitney tests. Results: In individuals with an increased level of cytotoxic CD8+ lymphocytes in peripheral venous blood had greater concentrations of leukocytes (7.4 ± 0.49 × 10 9 cells/l vs. 5.5 ± 0.23 × 10 9 , p = 0.003), lymphocytes (2.8 ± 0.17 × 10 9 cells/l vs.1.8 ± 0.07 × 10 9 cells/l, p = 0.005), and mature neutrophils (4.1 ± 0.19 × 10 9 cells/l vs. 3.4 ± 0.49 × 10 9 cells/l, p = 0.013). No associations between the level of apoptosis of lymphocytes (AnV+/PI-) and concentrations of sFasL, TRAIL, TNFa, and cytochrome C were observed. Conclusions: Lymphocytes CD8+ in healthy residents of the Russian North perform mainly cytotoxic function, which is not related to apoptotic cellular death.