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THE CHARACTERISTICS OF MITOTIC CHROMOSOMES AT CALENDULA OFFICINALIS L

MATERIALS AND METHODS Seeds of Pot Marigold were obtained from H l re ti village (Vaslui County), harvested in 2000. Germination (in average of 96%) was assured in Petri dishes, on filter paper moisturised with distilled water, at a medium temperature of 22 ± 2 C. When the little roots have reached 5 – 10 mm in length, the plantlets were treated with 0.2% colchicines, for 2 hours, at room temperature. After that, the plantlets were kept for another 2 hours on filter paper moisturised with distilled water. The settled (fixation) was assured for 16 hours in absolute ethylic alcohol / glacial acetic acid mixture (3 : 1 ), at room temperature. For hydrolysis was assured in 50%HCl, for 10 minutes, at room temperature. The staining was realised with Carr reactive. The microscopic slides were prepared by squash method. The metaphases were photographed with 100x immersion objective, by Nikon Eclipse 600 camera. Homologous chromosome groups were settled in accordance with the rapport between long arm and short arm, the mitotic index, the difference between arms and with the relative length of the chromosomes.