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Synthesis and Characterization of a Fullerenol Derivative for Potential Biological Applications

: Several biological barriers are generally responsible for the limited delivery of cargoes at the cellular level. Fullerenols have unique structural features and possess suitable properties for interaction with the cells. This study aimed to synthesize and characterize a fullerenol derivative with desirable characteristics (size, charge, functionality) to develop cell penetration vehicles. Fullerenol was synthesized from fullerene (C 60 ) solubilized in toluene, followed by hydroxylation with hydrogen peroxide and tetra-n-butylammonium hydroxide (TBAH) as a phase transfer catalyst. The obtained product was purified by a Florisil chromatography column (water as the eluent), followed by dialysis (cellulose membrane dialysis tubing) and freeze-drying (yield 66%). Subsequently, a silane coupling agent was conjugated on the fullerenol surface to render free amine functional groups for further covalent functionalization with other molecules. Characterization via UV–VIS, FTIR-ATR, Raman, DLS, and SEM techniques was conducted to evaluate the composition, size, morphology, surface functionality, and structural properties. We are currently working on the conjuga-tion of the potent cell-penetrating agents Buforin II (BUFII) and the Outer Membrane Protein A (OmpA) on the surface of the fullerenol to estimate whether cell penetration and endosome escape are improved concerning conventional polymeric vehicles and our previous developments with iron oxide nanoparticles.


Introduction
The nanocarriers' design for pharmaceutical applications one of the main challenges is to overcome several biological barriers as they are generally responsible for the limited delivery of functional cargoes to the target sites. The main consequence is the limited bioavailability of the pharmacological molecules, which is reflected in an increased number of dosages to reach the minimum effective concentration [1,2]. At the cellular scale, some of these barriers include the plasma membrane for the initial internalization and the endosomal one after cellular uptake. Moreover, for applications in gene delivery, the nanocarriers might need to come across the nuclear envelope [3,4]. One attractive strategy to address this challenge is the surface engineering of the nanocarriers with chemical functionalities that provide specific interactions with the constituents of the biological barriers to penetrate and eventually pass them [3][4][5][6][7]. Among many others, some of the molecules for functionalization include natural and synthetic polymers, translocating proteins and  peptides, phospholipids, and polysaccharides [3,8,9]. Depending on various physicochemical parameters of the nanoplatform (e.g., size, surface chemistry, charge, topology), the interactions with such molecules might vary significantly and alter their functionality [3,10,11]. As a result, the same molecule conjugated to different nanoplatforms might exhibit different interactions with subcellular compartments [12]. Over the past three years, we developed a new family of nanobioconjugates with cell-penetrating abilities and endosomal escape by immobilizing the antimicrobial peptide BUF-II and the biosurfactant protein OmpA on Iron Oxide nanoparticles [13][14][15]. We are currently exploring the impact of changing the nanostructured support on the translocating abilities and intracellular trafficking of the obtained nanobioconjugates. Accordingly, here we propose that the fullerenol derivatives may provide a suitable immobilization support for translocating molecules mainly due to their structural features, hydrophilic properties, and hollow spherical shape [6,16]. This work was, therefore, aimed at obtaining a fullerenol derivative with features that allow these potent cell-penetrating agents' conjugation.

Synthesis and Silanization of Fullerenol
Our fullerenol product was first prepared with a modification to a previously reported method [17]. To a solution of fullerene (100 mg) in toluene (50 mL), an aqueous solution of 30% hydrogen peroxide (10 mL) and tetra-n-butylammonium hydroxide, TBAH (40% in water, 500 µL) was added and stirred for 16 h at 60 °C. Subsequently, the aqueous phase was separated from the organic phase, and to eliminate residual TBAH, the aqueous phase containing the fullerenol was passed through a chromatographic column using Florisil as an absorbent and type II water as eluent. Then, to complete the purification, we combined dialysis (cellulose membrane dialysis tubing) and freeze-drying (yield: 66%) [18]. Next, 50 mg of fullerenol was dissolved in 15 mL of type II water. TMAH solution (500 µL, 25% (v/v)) and 25 µL of glacial acetic acid were then added to the solution and sonicated 10 min. APTES solution (500 µL, 20% (v/v)) was added to the fullerenol solution for silanization. The silanized fullerenol was washed with type II water to remove the excess of APTES [19]. Figure 1 shows a schematic of the synthesis procedure.

Fullerenol and Fullerenol Derivative Characterization
UV-VIS spectra were recorded with a GENESYS 10S UV-VIS spectrophotometer (Thermo Fisher Scientific Inc., Waltham, MA, USA). Infrared (FTIR) spectra were recorded with a Bruker Alpha II FTIR Eco-ATR (Bruker Optik GmbH, Ettlingen, Germany). Spectra were collected in the range of 4000-400 cm −1 with a spectral resolution of 2 cm −1 . Raman spectroscopy data were collected with a DXR Raman microscope (Thermo Fisher Scientific) system by exciting with the 532 nm line of an Ar ion laser. The exciting power at the sample was 8 mW with typical exposure time of 24 s and at least five repetitions. The system is equipped with a 900 lines per millimeter holographic grating. Nanoparticle size was determined through dynamic light scattering (DLS, Zeta-Sizer Nano-ZS; Malvern Instruments, Malvern, UK). Scanning electron microscopy (SEM) analysis of fullerenol was carried out in a TESCAN VEGA 3. First, fullerene was solubilized in toluene followed by hydroxylation with H2O2 and TBAH as a catalyst. The obtained product was purified by a Florisil chromatography column (water as the eluent) followed by dialysis (cellulose membrane dialysis tubing) and freeze-drying. Finally, the fullerenol was silanized with APTES to render free amine functional groups for further covalent functionalization with other molecules.

Results and Discussion
Since fullerene is insoluble in water, it was solubilized in toluene (purple coloration). After putting this solution in contact with the aqueous H2O2 solution, we observed two distinct liquid phases. TBAH is essential as it acts as a phase transfer catalyst by mediating the interaction between hydrophilic -OOH groups in the aqueous phase and the hydrophobic fullerenes in the organic phase. Once the reaction occurs, this organic phase becomes colorless and, upon standing still for 15 min, a yellow aqueous phase separates from the mixture (Figure 1) [8].
Fullerene in toluene and fullerenol in water UV-VIS absorption spectra are shown in Figure 2a. Fullerene dissolved in toluene has a purple coloration and characteristic absorption bands between 300 nm and 410 nm with a maximum at 283 nm, 335 nm, and 408 nm, followed by a broad absorption band in the range of 430 to 650 nm [20]. Fullerenol dissolved in water has a yellow coloration and is almost transparent in the visible region, due to its considerably decreased π-conjugation compare to fullerene. Figure 2b shows the infrared spectra of C60 fullerene (precursor), unpurified fullerenol (with TBAH residues), purified fullerenol, and silanized fullerenol. Fullerene has four characteristic infrared absorption bands of high intensity at 1429, 1182, 573, and 525 cm −1 due to C-C bonds (Figure 2b(1)) [21]. In the unpurified fullerenol, the two peaks observed at 2800-3000 cm -1 were attributed to residual TBAH (Figure 2b (Figure 2b(4)) [13]. The Raman spectrum of the purified fullerenol in Figure 2c shows characteristics bands between 1700-1000 cm −1 , which can be attributed to the C−C stretching vibrations [9]. However, the torsional motion of the C−O−H group is not visible. The fullerenols should have a diameter of ~1.0 nm but tend to aggregate easily, as seen in Figure 2d where the average hydrodynamic diameter approach 11 nm with a relatively narrow distribution [22][23][24]. SEM images of fullerenol in Figure 2e show flakes-like morphology similar to pristine fullerene, as reported elsewhere [25,26].

Conclusions
This synthesis and purification methodology proposed here allowed us to obtain a fullerenol derivative with free amine groups on the surface, which can be potentially exploited to conjugate the protein OmpA or the peptide BUFII via either amide or imine bonds. We are currently working on these conjugations to estimate whether cell penetration and endosome escape are improved concerning conventional polymeric vehicles and our previous developments with iron oxide nanoparticles.