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[Effects of various energy substrates on the recovery of the hepatic energy level after cold storage].

Effects of various energy substrates on the recovery of the hepatic energy level after 2 hour's cold storage were investigated. The following results were obtained. 1) Hepatic energy charge (EC) changed from 0.85 (normal value) to 0.42 after the end of 2 hour's cold stage. Hepatic EC recovered partially to about 0.65 due to reperfusion with the perfusate containing glucose (100 mg/dl). However, hepatic EC recovered no longer even with the perfusate containing glucose (200 mg/dl). 2) Hepatic EC recovered to almost normal value with the perfusate containing 3.3 mM alanine and glucose (100 mg/dl). In addition, the same recovery was observed with the perfusate containing 0.5mM palmitate and glucose (100 mg/dl). At the same time, increase of oxygen consumption was observed due to the addition of alanine on palmitate as compared with glucose in the perfusate. 3) No recovery was observed with the perfusate containing 1 mM palmitate and glucose. Also, more increase was observed in the oxygen consumption with the perfusate containing 1 mM palmitate than 0.5 mM palmitate. It is likely that uncoupling of mitochondria happened by high concentration of palmitate. As a result, glucose is is not sufficient for the recovery of hepatic EC after cold storage, however, physiological concentration of alanine or palmitate seemed to promote the recovery of the energy level to almost the normal level.