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EFFECT OF EXCHANGE TRANSFUSION AND CARBON MONOXIDE (CO) ON BRAIN AND HEART MITOCHONDRIA OF THE NEWBORN ANIMAL

Adult mitochondria show a high degree of adaptability to large changes of systemic PO2. The present studies illustrate response of mitochondrial respiratory activity in newborn piglets and lambs after exchange transfusions or rebreathing of 15% CO. Measurements of P50, PaO2 and SO2 were done in all animals. Brain and heart mitochondria were isolated, and State 4(substrate, no ADP)State 3(substrate ADP) respiratory rates were calculated as nmoles O2/min/mg protein, as well as respiratory control ratios(State 3 to State 4) and ADP/O ratios. All preparations were well coupled having respiratory control ratios about 10 and close to theoretical ADP/O ratios. Control animals from birth to 4 days old showed a linear decrease of State 3 respiratory rates from a mean of 120 to 55 nmoles O2/min/mg(46% of control) followed by a linear rise to the control level at 10 days of age. In the exchanged animals (low HbO2 affinity)State 3 respiratory rates increased by 38-90%. In the animals with COHb (high HbO2 affinity) changes of State 3 respiratory activity were more prominent in the brain than in the heart mitochondria, and were similar to changes observed during acute or chronic tissue hypoxia in adults. These data suggest that the respiratory activity of newborn mitochondria is sensitive to relatively small, acute changes of HbO2 affinity.