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In Vitro Studies of the Phenomenon of Tetracycline Incorporation into Enamel

Incorporation of tetracycline into dental enamel was studied by exposing presoftened enamel slabs to tetracycline-containing mineralizing solutions. Tetracycline was incorporated only when remineralization (assessed by hardness) occurred and the experimental conditions favored the formation of a soluble tetracycline-Ca complex. The fluorescence induced by tetracycline incorporation into the enamel slabs was comparable to that reported under in vivo conditions.