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Sustained inhibition of IL‐6 and IL‐8 expression by decoy ODN to NF‐κB delivered through respirable large porous particles in LPS‐stimulated cystic fibrosis bronchial cells

Cystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Neutrophil‐dominated inflammation and chronic bacterial infection are still considered the primary cause of bronchioectasis, respiratory failure and consequent death in CF patients. Activation of nuclear factor (NF)‐κB is responsible for overproduction of cytokines, such as interleukin (IL)‐6 and IL‐8, in airways of CF patients. Thus, decoy oligodeoxynucleotides against NF‐κB (dec‐ODN) may limit lung inflammation in CF. In the present study, we studied the effects of dec‐ODN delivered through biodegradable and respirable poly(D,L‐lactide‐co‐glycolide) large porous particles (LPP) on IL‐6 and IL‐8 mRNA expression as well as NF‐κB/DNA binding activity in cystic fibrosis cells stimulated with lipopolysaccharide (LPS) from Pseudomonas aeruginosa.