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Ir gene expression on T cells: Effect of a monoclonal antibody directed against I region‐controlled determinants on T cells

A monoclonal antibody directed at an I region‐controlled epitope uniquely expressed on T cells (Iat) was studied for its in vivo effect on the antibody response under the control of an Ir gene. The antibody was produced by a hybridoma made from A.TH spleen cells immune to A.TL (anti‐Ik), that was selected for its reactivity with T but not B cells and macrophages, and thus was designated as anti‐Iatk. The injection of this anti‐Iatk into H‐2k, H‐2b and H‐2k×bF1 mice resulted in the suppression of antibody response to poly‐L‐(His,Glu)‐poly‐D,L‐Ala–poly‐L‐Lys [(H,G)‐A–L] in H‐2k and F1 mice but not that to poly‐L‐(Tyr,Glu)‐poly‐D,L‐Ala–poly‐L‐Lys [(T,G)‐A–L] both in H‐2b and F1 mice. The adoptive cell transfer of the combinations of anti‐Iatk‐or normal mouse serum‐treated T and B cells into irradiated hosts demonstrated that anti‐Iatk primarily affected (H,G)‐A–L‐specific helper T cells but not B cells and macrophages, resulting in the specific elimination of the antibody response. Suppressor T cells were not induced by the treatment with anti‐Iatk. The antibody specifically eliminated the (H,G)‐A–L‐specific but not (T,G)‐A–L‐specific helper T cells in F1 spleen cells that had been primed with both (H,G)‐A–L and (T,G)‐A–L. The results indicated that anti‐Iatk affected the H‐2k‐associated Ir gene function born by T cells but not by antigen‐presenting cells, which was expressed on F1 helper T cells with apparent exclusion of the other allele, and implied that the Iat antigen on helper T cells is one of the sites of expression of Ir genes.