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Macrophage contact induces RhoA GTPase signaling to trigger tumor cell intravasation

Most cancer patients die as a result of metastasis, thus it is important to understand the molecular mechanisms of dissemination, including intra- and extravasation. Although the mechanisms of extravasation have been vastly studied in vitro and in vivo, the process of intravasation is still unclear. Furthermore, how cells in the tumor microenvironment facilitate tumor cell intravasation is still unknown. Using high-resolution imaging, we found that macrophages enhance tumor cell intravasation upon physical contact. Macrophage and tumor cell contact induce RhoA activity in tumor cells, triggering the formation of actin-rich degradative protrusions called invadopodia, enabling tumor cells to degrade and break through matrix barriers during tumor cell transendothelial migration. Interestingly, we show that macrophage-induced invadopodium formation and tumor cell intravasation also occur in patient-derived tumor cells and in vivo models, revealing a conserved mechanism of tumor cell intravasation. Our results illustrate a novel heterotypic cell contact mediated signaling role for RhoA, as well as yield mechanistic insight into the ability of cells within the tumor microenvironment to facilitate steps of the metastatic cascade.


Introduction
More than 1 in 3 people will develop cancer in their lifetime, and approximately 1,500 people die from cancer each day in the United States. Understanding the mechanistic basis of specific steps of metastasis is critical for the identification of robust early prognostic markers.
Understanding how tumor cells escape the primary tumor and enter the vasculature (a process termed intravasation) is a key step in designing treatment strategies of the disease. Multiphoton-based intravital imaging of rodent mammary adenocarcinoma (1) and human tumors (Patsialou et al, in prep) have revealed that tumor cells and macrophages cooperate during several key steps of the metastatic cascade. The polarization and subsequent motility of invasive tumor cells toward the blood vessels is dependent on a paracrine loop of signaling with macrophages (2,3). Tumor cells respond to macrophage-secreted epidermal growth factor (EGF), and in turn, macrophages respond to tumor cell-secreted colonystimulating factor-1 (CSF-1). Macrophages have also been shown to play a role in tumor cell entry into the vasculature in vitro and in vivo as treatment with drugs that affect macrophage function results in a decreased number of circulating tumor cells (1). However, as both the tumor cells ability to migrate toward the blood vessel as well as the actual intravasation step are both necessary for entry of tumor cells into the circulation, it is still unclear how macrophages affect tumor cell intravasation specifically. Furthermore, recent in vitro studies examining the mechanism of tumor cell intravasation has shown that the presence of macrophages increases tumor cell intravasation, but the mechanisms of this enhancement are still unclear (1,4).
Intravital imaging of the tumor microenvironment has shown that tumor cells intravasate into the vasculature at sites near macrophages (2). Due to these visualizations, a microanatomic landmark composed of a perivascular macrophage in contact with a tumor cell at blood vessels had been identified and termed TMEM, or Tumor MicroEnvironment of Metastasis. In a case-controlled study of metastastic and nonmetastatic breast cancers, TMEM density in breast tumors at initial resection was associated with risk of metastasis (5), suggesting that macrophages, tumor cells and endothelial cells cooperate for tumor cell entry into the vasculature. However, little is known about the cell biological mechanisms that exist between these three cell types during intravasation.
To gain a closer look at the molecular mechanisms of tumor cell transendothelial migration, in vitro experiments focused on studying tumor cell and endothelial cell behavior have been widely studied. Often, models of tumor cell extravasation, or exit of tumor cells from the vasculature, are used since the apical surface of the endothelial can be easily accessed by plating endothelial monolayers and seeding tumor cells on these monolayers (6)(7)(8)(9)(10). From these studies, an extensive view of how tumor cells affect the endothelial cell architecture has been elucidated, and the specific steps of tumor cell adhesion and intercalation during transmigration have been described (6,7,11,12). A common downstream mechanism of tumor cell transendothelial migration is the opening of the endothelial monolayer. Endothelial cells lose their adherens junctions and tight junctions as gaps open in the monolayer, allowing for tumor cell passage. Molecular pathways leading to adhesion dissolution have also been identified (11,13,14). What is less clear, however, is how tumor cells undergo intravasation, and whether common mechanisms exist between intravasation and extravasation. Given that tumor cells withstand shear flow stresses in the blood vessels and that different cells are present at sites of intravasation and extravasation, we predict that different modes of cell-cell interactions occur when tumor cells intravasate versus extravasate. As such, given that macrophages are visualized at sites of tumor cell intravasation in vivo, we sought to explore the link between the close association of macrophages and tumor cells, and the subsequent tumor cell intravasation.
Using dissolution of endothelial cell-cell adhesion as a readout of tumor cell transendothelial migration, we dissected the relationship between macrophages and tumor cells with an in vitro model of intravasation. In particular, we asked if the association of macrophages and tumor cells reflected a cell biological mechanism that supports intravasation. Using high resolution imaging, combined with in vitro and in vivo models, we tested whether macrophages enhance tumor cell intravasation and the cell biological and signaling mechanisms that regulate this process. Our results reveal that macrophages facilitate tumor cell intravasation by inducing the activation of the RhoA signaling pathway resulting in the formation of invadopodia, enabling the tumor cells to initiate intravasation by penetrating through the basement membrane of the vasculature.

Validation of in vitro intravasation assay
To specifically examine the interactions between tumor cells, macrophages and endothelial cells during tumor cell intravasation, we developed an in vitro model to mimic the appropriate endothelial monolayer polarity. Using primary human microvascular endothelial cells (HUVECs), we formed "upside down" endothelial monolayers using transwells for support ( Figure 1A). The endothelium was intact, impermeable to large molecules, and displayed high transendothelial electrical resistance ( Figure S1A, B). To image the transwells, transwells were placed in glass-bottom dishes and imaged en face at the level of the endothelium ( Figure 1A, Figure S1C). We assessed monolayer polarity by immunostaining the endothelium with polarity markers, apical ZO-1 and basal collagen IV, and found that proper polarity was established, with the basal side of the endothelium facing the transwell and the apical side of the endothelium facing the bottom chamber (Figure S1C-F).

Macrophage enhance tumor cell intravasation in vitro
To examine the interactions between tumor cells, macrophages and the endothelium during intravasation, we added a highly metastatic triple negative human breast cancer cell line, MDA-MB-231, and the BAC1.2F5 murine macrophage cell line to the transwells and analyzed the interactions with fixed and live imaging ( Figure 1). We have previously shown that the interactions between these cells are not hindered due to species difference (15,16). With fixed imaging of en face views of the transwells, we found that tumor cells and macrophages made close interactions with each other at the level of the endothelium ( Figure  1B). Live imaging of tumor cell transmigration in the transwells revealed that tumor cells preferentially underwent transendothelial migration at sites where macrophages were present ( Figure 1C, D; Movie S1). Tumor cells took approximately 2-3 hours to undergo transendothelial migration ( Figure 1C), consistent with published in vivo and in vitro studies indicating that tumor transmigration occurs on the order of several hours (4,(17)(18)(19)(20) in contrast to neutrophil migration which occurs on a faster time scale (21).
To determine whether macrophages facilitated tumor cell intravasation in our assay, we compared tumor cell transmigration in transwells in which macrophages were present or absent. Tumor cells exhibited a basal level of intravasation independently of macrophages, however tumor cells exhibited a 3-fold increase in intravasation in the presence of macrophages (Figure 2A-C). We tested several macrophage to tumor cell ratios in our assay and determined that a four-fold increase in macrophages to tumor cell ratio elicited a robust tumor cell intravasation response ( Figure S2A). As the BAC1.2F5 macrophage cell line was used for these experiments, we also tested for the ability of other macrophage cell lines to enhance tumor cell intravasation. Using an immortalized bone marrow-derived macrophage cell line (iBMM) (22) as well as the RAW264.7 macrophage cell line, we found that tumor cell transendothelial migration was enhanced ( Figure S2B), suggesting that this phenomenon was due to a general mechanism by macrophages and not specific to a particular macrophage cell line. As BAC1.2F5 macrophages generated the most consistent and robust macrophage-induced response, this cell line was used for the remainder of the study.
Although tumor cells exhibited a basal level of intravasation independently of macrophages ( Figure 2A, C), we specifically were interested in how macrophages affected tumor cell transmigration since there was a strong correlation between TMEM formation and distant metastases in patients (5). Therefore, we focused our studies on macrophage-induced transendothelial migration. To determine the effects of tumor cell transendothelial migration on endothelial cell-cell adhesion, we examined cell adhesion in transwells during tumor cell crossing. Using multiphoton-based intravital imaging, tumor cell protrusions aligned along endothelial cell-cell contacts in vivo, suggesting that these protrusive structures were probing the endothelial cell adhesions ( Figure S3). Indeed tumor cells have been shown to insert into endothelial monolayers between endothelial cells in in vitro models (6). Consistent with these in vivo results, in our in vitro intravasation assay, endothelial cells lost cell-cell adhesion in regions of tumor cell intravasation ( Figure 2D), suggesting that tumor cells were undergoing paracellular migration (between endothelial cells) versus transcellular migration (through endothelial cells) ( Figure 2D). This paracellular migration was also reflected in live movies in which tumor cells were visualized to migrate between endothelial cells ( Figure 1C). To determine whether the observed paracellular migration was induced by macrophages, we quantified the number of paracellular migration events that occurred when tumor cells were in contact with macrophages versus those that were independent of macrophages. Tumor cells were seen to be in contact with macrophages 86% of the time during paracellular transmigration ( Figure 2E), suggesting that the intravasation is a macrophage-induced phenomenon.

Macrophages induce invadopodium formation in tumor cells upon physical contact
From the above results, we determined that the presence of macrophages correlated with tumor cell intravasation; therefore, we next sought to determine the cell biological mechanism by which this induction occurred. We hypothesized that tumor cells must degrade the basal lamina of the endothelium during transmigration. Since breast cancer cell lines were known to form invadopodia, actin-rich structures that degrade matrix, we tested whether macrophages were capable of inducing the formation of invadopodia in breast cancer cells during transendothelial migration, as the formation of invadopodia during this process has been speculated to occur in vivo and in vitro, but never unequivocally shown (23)(24)(25)(26). To address this question, we first assayed for the formation of macrophage-induced invadopodia in 2D, and then assayed for the formation of macrophage-induced invadopodia during in vitro intravasation.
In our 2D assay, the formation of invadopodia was detected by subcellular punctate structures formed at the ventral surface of cells that are positive for two invadopodium markers, tks5 and cortactin (27,28). Tks5 is an adaptor protein necessary for invadopodium formation (29) and cortactin is an actin-binding protein shown to localize and function at invadopodia (27,28,30,31). MDA-MB-231 cell lines have been shown to form invadopodia readily when plated in full serum and/or upon EGF stimulation, even in the absence of macrophages (27,28,32,33). To study the role of macrophages in the formation and function of invadopodia in tumor cells, we first serum-starved the tumor cells and then introduced macrophages. When MDA-MB-231 cells were in serum-starvation conditions, they form few invadopodia, with a majority of the cells forming none at all (control panels; Figure 3A,B). However, when serum-starved MDA-MB-231 cells were cocultured in the presence of macrophages, we found that tumor cells in direct contact with macrophages formed an average of five functional invadopodia, that contained both cortactin and tks5 invadopodium markers and were capable of degrading matrix ( Figure 3A,B). These findings were in contrast to the average number of invadopodia formed by tumor cells not in physical contact with macrophages ( Figure 3A), which was similar to that of tumor cells that were plated alone ( Figure 3B). These experiments suggest that direct physical contact was necessary between the tumor cells and macrophages for macrophage-induced invadopodium formation. To confirm this hypothesis, we performed macrophage conditioned media experiments to stimulate tumor cells. Macrophage conditioned media did not increase the number of invadopodia compared to control media ( Figure 3C), further suggesting that physical contact between macrophages and tumor cells was required for enhanced invadopodium formation.

Macrophage-induced invadopodia form and are necessary for tumor cell intravasation
Since the formation of invadopodia was induced by macrophages on 2D matrices, we next tested whether invadopodia could be induced by macrophages during tumor cell intravasation. Tumor cells were first transfected with cortactin-tagRFP to assess the formation of invadopodia. Regions of tumor cell transendothelial migration were detected by a loss of endothelial cell-cell adhesion, as visualized by tight junction immunostaining. Tumor cells indeed accumulated the invadopodium marker, cortactin, in areas of the tumor cell that penetrated through endothelial barriers ( Figure 4A, red arrow) forming an invasive front similar to that previously shown in three dimensional collagen matrices (32,34), suggesting that invadopodia formed during intravasation. We also found that an independent invadopodium marker, Tks5, is also enriched in areas of the tumor cell that penetrate the endothelium ( Figure S4A, green arrow). To determine whether invadopodia are indeed necessary for efficient intravasation, tks5 was knocked down in tumor cells and assessed for their ability to undergo intravasation. Consistent with published results, cells with either transient or stable knockdown of tks5 did not form invadopodia on 2D substrates (29); Figure 4B-E; Figure S4B,C). Transient or stable Tks5 KD cells also did not undergo intravasation even in the presence of macrophages and their level of crossing was similar to background levels ( Figure 4F; Figure S4D). Furthermore, treating tumor cells with an MMP inhibitor, GM6001, which has been shown to inhibit the ability of invadopodia to degrade matrix (31), resulted in the inability of tumor cells to cross the endothelium ( Figure 4F,G), further suggesting invadopodia are required for intravasation. Given that tks5 KD cells do not exhibit motility defects (29), these experiments strongly suggest that the matrix degrading ability of macrophage-induced invadopodium formation is necessary for tumor cell intravasation.

Patient-derived breast tumor cells form invadopodia and physically contact macrophages during tumor cell intravasation
As the MDA-MB-231 breast cancer cell line is triple negative for the estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2, we sought to validate our findings with another triple negative receptor cell line. To add clinical relevance to our findings, we used breast tumor cells derived from a patient with triple negative breast cancer, TN1, which are capable of invasion and spontaneous metastasis (35). Since these primary cells do not grow in culture as a cell line, TN1 tumors were grown orthotopically in mice and the tumor cells were dissociated and assayed for their ability to undergo macrophage-dependent transendothelial migration and invadopodium formation in vitro.
We found that TN1 cells exhibited macrophage-induced invadopodium formation (Figure 5A-D; Figure S5). We further found that TN1 cells preferentially underwent intravasation when in contact with macrophages, consistent with our findings with MDA-MB-231 cells ( Figure 5E-I). When we quantified the amount of transmigration that occurred, we found that 86% of TN1 cells physically contacted macrophages when undergoing paracellular migration, which was similar to our findings with MDA-MB-231 cells ( Figure 5I, compare to Figure 2E). Thus, our experiments with patient-derived breast tumor cells recapitulate our in vitro intravasation results with cell lines.

Physical contact between macrophages and tumor cells correlate with invadopodium formation in vivo
Our in vitro results with both cell lines and patient-derived cells suggest that contact between macrophages and tumor cells induce invadopodium formation in tumor cells to facilitate tumor cell intravasation. To validate these findings in vivo, we generated orthotopic MDA-MB-231 tumors in immune-compromised mice. Once tumors reached 1-1.2cm, the tumors were removed, sectioned and immunostained for blood vessels, macrophages and invadopodia. Specifically, we tested whether tumor cells upregulate invadopodia markers when in physical contact with macrophages near blood vessels in vivo. We found that this was indeed the case, and tumor cells that were in physical contact with macrophages formed cortactin-rich structures next to blood vessels, compared to cells at the blood vessel that were not in contact with macrophages ( Figure 6A,B). These experiments suggest that physical contact between macrophages and tumor cells induce invadopodium formation at blood vessels in vivo.

Macrophages activate RhoA signaling in tumor cells to induce invadopodium formation upon physical contact
We have shown that macrophages induce tumor cell invadopodium formation upon physical contact for intravasation. We next sought to determine the molecular mechanism of this heterotypic cell contact. RhoA, a member of the Rho family of GTPases, was shown to be involved in invadopodium formation (36,37), and RhoC GTPase has been shown to affect invadopodium morphology and function without affecting invadopodium number (37). As we had visualized changes in invadopodium number upon macrophage contact, we hypothesized that RhoA activity may be induced upon contact with macrophages. Furthermore, RhoA acts as a central node of signaling when cells are mechanically stimulated during processes such as cell-cell interactions (38). Consistent with previous data revealing that knocking down RhoA expression led to reduced matrix degradation (36), we found that knocking down RhoA expression in tumor cells reduced invadopodium formation ( Figure S6A,B) and inhibited the ability for macrophages to induce invadopodium formation ( Figure S6C). We further found that knocking down RhoA expression resulted in decreased tumor cell intravasation in vitro ( Figure S6D). We next tested whether constitutive active mutations of RhoA was sufficient to induce invadopodia in the absence of macrophage contact and found that this was indeed the case, as tumor cells expressing either G14V-RhoA or F30L-RhoA constitutive active mutations formed more invadopodia than WT RhoA in the absence of macrophage contact ( Figure S6E). We next used a RhoA biosensor in tumor cells to monitor the RhoA activation in real time upon physical contact with macrophages. RhoA biosensor expressing cells that did not contact macrophages maintained a constant level of RhoA activity ( Figure 7A, C; Figure  S7A; Movie S2, S3). However, when macrophages contacted tumor cells ( Figure 7B, top DIC panels and inset), there was a global increase in RhoA activity that was sustained for 30 minutes ( Figure 7C; Figure S7B; Movie S4, S5). The increase in RhoA activity was concomitant with multiple cell protrusions globally around the tumor cell ( Figure 7B, Figure  S7B; white arrows), a previously described indicator of increased RhoA activity (39). To determine whether this global increase in RhoA activity led to the formation of invadopodia, we first imaged RhoA biosensor-expressing tumor cells for RhoA activity, and then subsequently assessed the same cells for their ability to form invadopodia upon RhoA activation. We found that tumor cells that were in contact with macrophages and exhibited a global increase in RhoA activity formed significantly more invadopodia than control ( Figure  7D, E). Furthermore, these experiments suggest that macrophages induce RhoA GTPase signaling in tumor cells to trigger invadopodium formation for intravasation.

Discussion
While there is a breadth of research exploring how tumor cells affect endothelial cell-cell adhesion, and cytoskeletal mechanisms of extravasation has been vastly studied, little is known about the initial steps of tumor cell intravasation and how other cells within the tumor microenvironment affect this process. Our experiments yield an unexpected finding that macrophages induce RhoA activity and the subsequent enhanced invadopodium formation in breast cancer cells, and that this induction requires physical contact between the two cell types. We have further shown that this macrophage-induced invadopodium formation facilitates tumor cell intravasation using breast cancer cell lines and patientderived tumor cells, consistent with the hypothesis that invadopodium formation is necessary for tumor cells to degrade the basal lamina overlying blood vessels. These experiments yield mechanistic insight into the formation of TMEM sites in patients that develop distant metastasis. The tripartite structure involving tumor cells, macrophages and endothelial cells found in patients developing distant metastasis may reflect the cell biological mechanism of macrophage-induced invadopodia described in this study.

Invadopodium formation is the first step in tumor cell intravasation
Our results reveal that invadopodia form and are necessary for intravasation. While this phenomenon has been alluded to in the literature, it has never been clearly shown. In vivo studies show that knocking down genes required for invadopodium formation such as N-Wasp, Arg, tks5, or overexpressing proteins resulting in excess invadopodia, result in decreased distant metastasis and/or number of circulating tumor cells (23,(40)(41)(42). Several steps are required from primary tumor growth to distant metastasis, so it is difficult to ascertain which specific step of the metastatic cascade is perturbed when tumor cells are unable to form invadopodia. By taking an in vitro approach, we are able to show that invadopodia do indeed form during transendothelial migration, and that invadopodia are also necessary for tumor cell transendothelial migration to occur. Furthermore, we confirm our findings in vivo and show using a blood vessel marker to clearly demarcate where vessels lie in the tumor, tumor cells form invadopodia at sites of intravasation. Interestingly, invadopodia have been suggested to be dispensable for extravasation in vivo (42), revealing a potentially marked difference between mechanisms of intravasation versus extravasation.
Previous experiments suggest that upon tumor cell attachment onto the endothelium, endothelial cells activate Src and other signaling cascades such as p38 and ERK1/2 leading to decreased endothelial cell-cell adhesion, and changes in the endothelial cell actin architecture to facilitate tumor cell crossing (43)(44)(45)(46)(47)(48)(49). Whether invadopodia are required to directly disassemble cell-cell adhesion is unknown. However, we hypothesize that the interaction between perivascular macrophages and tumor cells to induce invadopodium formation comprises only the first step in tumor transmigration, and that invadopodium formation is necessary for the tumor cells to penetrate through the basement membrane of the blood vessel to then make contact with the underlying endothelial cells. Subsequent downstream steps requiring loss of endothelial cell-cell adhesion is necessary for completion of tumor cell crossing. Tumor cells also adopt changes in their cytoskeleton to facilitate transmigration (11,13,50). Consistent with previous endothelial cell-cell adhesion studies, we have shown that endothelial cells clearly detach from one another as tumor cells cross through the monolayer, and that tumor cells primarily undergo paracellular transendothelial migration in our in vitro assays.

The role of tumor associated macrophages during tumor cell intravasation
Macrophages are critical players in metastasis, and several macrophage subtypes reside within the tumor microenvironment. We hypothesize that the perivascular macrophages comprise a specific macrophage subtype that facilitates tumor cell intravasation by inducing invadopodium formation in the tumor cell. This does not preclude any other role for macrophages in inducing perturbations in the blood vessels, or signaling that may occur between macrophages and endothelial cells during intravasation. Multiphoton based intravital imaging reveals that while tumor associated macrophages are highly motile, the perivascular macrophages remain stationary, closely docked at blood vessels, and that in some instances, perivascular macrophages can be seen in close association with intravasating tumor cells (3). In this work, we show that macrophages induce RhoA activation and the subsequent formation of invadopodia during transmigration. Certainly, other cell types in the tumor microenvironment also affect tumor cell invadopodium formation and intravasation ( Figure S8), revealing the complexity of the tumor microenvironment. Interestingly, only those cell types tested that are capable of inducing invadopodium formation in tumor cells are also capable of enhancing tumor cell intravasation ( Figure S8). We predict that invadopodium formation is necessary for several steps of the metastastic cascade, and that there will be different levels of regulation by macrophages and other factors in the tumor microenvironment at each of these steps.

A novel heterotypic cell contact role for RhoA signaling
Recent advances in imaging RhoA activity in vivo reveal subcellular spatial regulation of RhoA activity in cancer models (51,52). Consistent with these findings, we reveal that macrophages and tumor cells require physical cell-cell contact to induce RhoA activation and subsequent invadopodium formation. GTPases are involved in the formation of homotypic cell-cell contacts in vivo and in vitro, mainly though regulating the integrity of junctions within a sheet of cells (53)(54)(55)(56)(57). While RhoA activity has been shown in nascent homotypic cell-cell contacts (58), it remained unclear how heterotypic cell contacts regulate RhoA activity in real-time. Additionally, although the EGF/CSF-1 paracrine loop of signaling was identified between tumor cells and macrophages, the intracellular signaling pathways induced by macrophages in the tumor microenvironment were elusive. Indeed, the EGF/CSF-1 paracrine loop of signaling is also required for both macrophage-induced invadopodium formation and transendothelial migration ( Figure S9). However, as these are known to be secreted molecules, it remains to be determined which upstream contactmediated signaling between cells in the tumor microenvironment is important for invadopodium formation during transmigration. We hypothesize that the yet unidentified contact mediated ligand-receptor pair will activate the RhoA pathway, resulting in increased invadopodium formation in tumor cells at blood vessels. Our results illustrate a novel role for RhoA in real-time in heterotypic cell-cell contact signaling. The global RhoA increase in the tumor cell, not just at the site of cell contact, suggests that RhoA signaling stimulates invadopodium formation, not merely the location where invadopodia will form. Work exploring upstream signaling pathways regulating RhoA activity during intravasation is currently underway.

Clinical significance of macrophage-induced intravasation
Using patient-derived breast tumor cells, we confirmed our findings that macrophages induce both invadopodium formation and intravasation in vitro. Broadly, the close association of macrophages and tumor cells at the level of the endothelium lends credence to the finding of TMEM sites in resected tumor tissue of breast cancer patients. Thus, our results support the value of using the number of TMEM sites as a prognostic marker of the risk of distant metastasis. Human umbilical vein endothelial cells (HUVECs, Lonza) were cultured in EGM-2 (Lonza) and only used between passage 2-4. Immortalized bone marrow-derived macrophages (22) were cultured in 10% FBS/MEM supplemented with 2 mM L-glutamine, 22 μg/mL Lasparagine, and 10 000 U/mL of purified human recombinant CSF-1. RAWs cells were cultured in 10% FBS/RPMI. HL-60 cells were cultured and differentiated as described (59). TN1 cells were isolated and stably labeled to express GFP as described (35) and maintained by passage through orthotopic injections of mice (Supplementary Materials and Methods).

DNA siRNA and transfection and cell labelling
1×10 6 MDA-MB-231 cells were transfected by 2μg each of Cortactin-tagRFP (27) and GFP-tks5 (kindly provided by Sara Courtneidge), or 1.5μg each of RhoA-WT, RhoA-F30L, RhoA-G14V using the Lonza Nucleofection Kit V protocol 24 hours prior to the experiment using manufacturer conditions. Control nonsilencing siRNA was from Qiagen. Humanspecific tks5 and RhoA siGenome Smart Pool were from Dharmacon. 1×10 6 MDA-MB-231 cells were transfected with 2μM siRNA using the Lonza Nucleofection Kit V 72 hours (for tks5) and 96 hours (for RhoA) prior to each experiment. Immunoblot analysis was used to confirm knockdown for each experiment. BAC1.2F5 and HUVECs were labeled with cell tracker dyes (CMFDA, CMPTX from Invitrogen) prior to experiments. Stable cell lines or MDA-MB-231-EGFP and MDA-MB-231-dTomato were made as described (15), with the exception that dTomato was inserted into the EGFP site in the EGFP-C1 vector (Clontech).

Cloning RhoA constitutive active mutants
Expression constructs for the RhoA F30L and G14V mutants were produced and cloned into the pTRIEX-4 backbone (Novagen) as described (Supplementary Materials and Methods).

Inhibitors and blocking antibodies
For in vitro transendothelial migration and invadopodia formation assays, the mouse CSF-1 receptor was blocked with antimouse CSF-1R blocking antibody (AFS98, Novus Biologicals) at a concentration of 5μM, and a Rat IgG1 k isotype control blocking antibody was also used at 5μM. To block the EGF receptor, Iressa (AstraZeneca) was used at 5μM. To block matrix metalloproteases, GM6001 (BML-E1300-0001, Enzo Life Sciences) was used at 5μM.

Western Blotting and quantification
Cells were transfected with tks5, RhoA, or control siRNA for 72 or 96 hours, lysed with SDS-PAGE sample buffer, sonicated and boiled at 95°C. To maintain TN1 cells, tumors of approximately 1 cm in diameter were excised and trimmed, mechanically dissociated with scalpels, and then enzymatically digested for 1 hour at 48°C (Liberase TH, Roche). Samples were filtered twice into a single cell suspension in PBS/2% FBS, red blood cells lysed with ACK buffer (Invitrogen, A10492-01), and cell washed twice with PBS/2% FBS on ice.

Invadopodium assay
405 gelatin-labelled Mattek dishes were made as previously described (60). Tumor cells were plated in complete media for 4 hours on Alexa 405 labelled gelatin dishes. Dishes were fixed and immunostained for cortactin and tks5 as previously described (27). Cells were imaged with a Deltavision Core Microscope (Applied Precision) using a CoolSnap HQ2 camera, 60X 1.42 N.A. oil immersion objective, and softWorx software. Fixed cells were imaged in PBS at room temperature. Invadopodia were detected as punctate structures that were positive for both cortactin and tks5, and capable of degrading Alexa 405-gelatin.

Macrophage-induced invadopodia assay
MDA-MB-231 and TN1-GFP cells were serum starved for 16 hours. BAC1.2F5 cells were cell tracker labeled (CMPTX, Invitrogen). 25K MDA-MB-231 cells were incubated with 125K BAC1.2F5 cells in serum starvation media on 405-labelled gelatin coated dishes for 4 hours, fixed and immunostained for invadopodia markers as described above. For conditioned media experiments, BAC1.2F5 cells were incubated with complete media, with the exception that 300 U/mL CSF-1 was used rather than 3 000 U/mL CSF-1, for 24 hours, and subsequently used for assays. Unconditioned complete media with 300 U/mL CSF-1 was used as a control.
To assess other cell types' ability to induce tumor cell invadopodia, 125K of HL-60 or Jurkat T-cells were incubated with 25K MDA-MB-231 cells in serum starvation media as described above.

In vitro intravasation assay
8-micron pore sized transwell inserts (Millipore) were inverted and the filter was plated with 50uL of 2.5 μg/mL of growth factor reduced matrigel (BD) in DMEM media (Invitrogen) for 1 hour at room temperature. Excess matrigel was removed, and 100K HUVECs were plated on the matrigel-coated filters on the inverted transwells in 50μL EGM-2 for 4 hours in a 37°C CO2 incubator. Transwells were then flipped right side up into a 24 well plate (Corning) and tumor cells and macrophages or other cell types were added as described (Supplementary Materials and Methods). To assess the extent of tumor cell transendothelial migration, transwells were washed twice with PBS and then fixed in 4% paraformaldehyde/PBS for 20 minutes, permeabilized 0.1% triton X-100 for 10 minutes and stained with rhodamine-phalloidin (Invitrogen) and DAPI for 1 hour. Transwells were placed in PBS on a Mattek dish (MatTek Corp) and imaged on an inverted Multiphoton Olympus FV1000-MPE microscope with a 25X NA 1.05 water immersion objective and a Spectra Physics Mai Tai-DeepSee laser set to 880nm. 2μm step Z-stacks of en face views were acquired throughout the depth of the transwell, and only those tumor cells that breached the endothelium were scored as a positive transendothelial migration event. Approximately 6 fields of view were acquired for each transwell.
Transwells were immunostained with ZO-1 (Invitrogen) and Collagen IV (Abcam) antibodies following a previously described protocol (61) and imaged as described (Supplementary Materials and Methods).
To live image the transwells, transwells were set up as described above, and prior to imaging on an inverted Multiphoton Olympus FV1000-MPE microscope with a 25X NA 1.05 water immersion objective and a Spectra Physics Mai Tai-DeepSee laser set to 880nm, the transwells were placed in Mattek dishes with L-15/10% FBS. 2 micron step Z-stacks, encompassing the most apical 10 microns of the transwells were taken every 10 minutes for 3 hours.

Transwell permeability and TEER measurements
Endothelial monolayers were grown on transwells as described above. 1mg/mL 60K texas red dextran was added to the top well. 50uL of media was taken from the bottom well after 30 and 60 minutes, and fluorescence measurements were determined with the described settings for costar 96-well plates, 555/640 using a Fluostar Optima. Transendothelial electrical resistance measurements were made on the same transwells using an EVOM2 epithelial Voltohmmeter (World Precision Instruments).

In vivo imaging and immunostaining
All experiments conducted in mice were in accordance with the National Institutes of Health regulation on the use and care of experimental animals and approved by the Albert Einstein College of Medicine Animal Use Committee. MDA-MB-231 orthotopic tumors were generated by injected MDA-MB-231 cells expressing GFP in sterile PBS/20% collagen I into the mammary fat pad of severe combined immunodeficiency mice (SCID) mice (NCI, Frederick, MD). Tumors grew for approximately 3 months until they reached 1 cm in size. To image tumor cell interactions with the blood vessel in vivo, mice were injected by tail vein with 50 μL of rhodamine-labelled Ricinus Communis Agglutinin I (Vector Labs). Animals were sacrificed immediately after injection, the tumors were removed and imaged in PBS on an Olympus FV1000-MPE microscope with a 25X NA 1.05 water immersion objective and a Spectra Physics Mai Tai-DeepSee laser set to 880nm. 2um step size Z-stacks were taken for each field of view. For immunostaining of tumor sections, tumors were removed and frozen on dry ice in OCT Compound (TissueTek), sectioned, fixed, immunostained and imaged as described (Supplementary Materials and Methods).

RhoA Biosensor Imaging and Quantification
MDA-MB-231 cells were transiently transfected with the RhoA biosensor (39) using the Lonza Nucleofection protocol described above and plated directly onto MatTek dishes, serum starved overnight, and imaged in a single plane for 3-color imaging every 2 minutes using a previously described protocol (37,62). Ratiometric calculations on the CFP and YFP FRET emissions of RhoA were performed as described (37,39). Cells were subsequently fixed and immunostained using methods described above and quantified with the DeltaVision Core microscope using methods described above. For more information, see Supplementary Materials and Methods.

Statistical Analysis
Statistical analysis was conducted using an unpaired, two-tailed Student's T-test when comparing two data sets. Statistical significance was defined as a p < 0.05. For multiple comparisons, ANOVA was used. All graphs are displayed as mean ± SEM, unless otherwise indicated. The "n" in SEM was derived from number of fields of view (for tumor cell intravasation) or number of cells (for invadopodium formation).

Supplementary Material
Refer to Web version on PubMed Central for supplementary material.