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Photochemically induced dynamic nuclear polarization NMR on photosystem II: donor cofactor observed in entire plant

The solid-state photo-CIDNP (photochemically induced dynamic nuclear polarization) effect allows for increase of signal and sensitivity in magic-angle spinning (MAS) NMR experiments. The effect occurs in photosynthetic reaction centers (RC) proteins upon illumination and induction of cyclic electron transfer. Here we show that the strength of the effect allows for observation of the cofactors forming the spin-correlated radical pair (SCRP) in isolated proteins, in natural photosynthetic membranes as well as in entire plants. To this end, we measured entire selectively 13C isotope enriched duckweed plants (Spirodela oligorrhiza) directly in the MAS rotor. Comparison of 13C photo-CIDNP MAS NMR spectra of photosystem II (PS2) obtained from different levels of RC isolation, from entire plant to isolated RC complex, demonstrates the intactness of the photochemical machinery upon isolation. The SCRP in PS2 is structurally and functionally very similar in duckweed and spinach (Spinacia oleracea). The analysis of the photo-CIDNP MAS NMR spectra reveals a monomeric Chl a donor. There is an experimental evidence for matrix involvement, most likely due to the axial donor histidine, in the formation of the SCRP. Data do not suggest a chemical modification of C-131 carbonyl position of the donor cofactor.

Scientific REPORTS | (2018) 8:17853 | DOI: 10.1038/s41598-018-36074-z two large trans-membrane protein complexes, photosystem 2 (PS2) and photosystem 1 (PS1), operate in series (for a review, see Blankenship 4 ). In plant cells, both PS2 and PS1 are located in the thylakoid membrane in the chloroplasts, pumping protons from the lumen to the stroma site. For structural and functional studies on PS2, it is possible to selectively remove PS1 from the membrane to obtain PS2 enriched membranes, so-called BBY preparations (Berthold, Babcock and Yocum) 5 . From these membranes, the PS2 core particles (Fig. 1a) can be isolated by removing peripheral light-harvesting complex 2 (LHC2) using Triton X as detergent as described by van Leeuwen et al. 6 . Further removal of the light-harvesting core antenna proteins CP43 and CP47 leads to the PS2 RC or D1D2 complex (Fig. 1b) comprising the D1 and D2 polypeptides in which the two branches of cofactors are symmetrically arranged (Fig. 1c). The cofactors consist of two inner chlorophyll a (Chl a) molecules (P D1 and P D2 ), two accessory chlorophylls (Chl a D1 and Chl a D2 ), two pheophytins (Phe a) and two quinones (Q) arranged in two symmetric branches. In addition, the PS2 RC contains two β-carotenes, two peripheral chlorophylls (Chl a p1 and Chl a p2 ) and the water splitting manganese cluster, also known as the oxygen-evolving complex (OEC). PS2 has the highest oxidation power known in living nature (+1.2 V), allowing to drive the water splitting. On the other hand, PS1 has the strongest reductive power known in living nature, which is required for the CO 2 reduction. How PS2 is able to produce and maintain such oxidative strength within a protein environment and without changing the HOMO-LUMO gap (680 nm) is, despite extensive research by optical-kinetic and magnetic resonance techniques and the availability of high-resolution (1.9 Å) x-ray data, not yet fully understood. Figure 1. The PS2 core complex (a) is embedded inside the thylakoid membrane (not shown). At the heart of the core complex, the PS2 reaction center (RC) is found (b) and mainly formed by the central D1 and D2 polypeptides. The PS2 RC contains several cofactors (c): two central Chls (P D1 and P D2 ), two accessory Chls (Chl D1 and Chl D2 ), two pheophytins (Phe D1 and Phe D2 ), two quinones (Q A and Q B ), and two peripheral Chls (Chl P1 and Chl P2 ). These cofactors are arranged in two symmetrical branches, an active D1 branch (left) and an inactive D2 branch (right). Two β-carotenoids (Car D2 and Car D1 ) are associated with the PS2 core complex. At the P D1 side, a tyrosine residue Tyr Z is in between P D1 and the oxygen evolving system (OEC). Scientific  Until now, photo-CIDNP MAS NMR studies on plant PS2 have been restricted to experiments on isolated D1D2 RC preparations from spinach (Spinacia oleracea) due to the difficulty to incorporate selective isotope labels into plant RCs. Based on the data obtained from natural abundance D1D2, a first assignment of the light-induced signals was made and the donor was identified to be a single Chl a cofactor 7,8 . The intensity pattern provided strong evidence for a pronounced asymmetry of the electronic spin-density distribution within the monomeric Chl a donor assigned to P D1 . While free Chl a in solution has the highest electron-spin density being located at pyrrole ring II 9 , the Chl a donor inside the D1D2 complex shows a shift of electron-spin density towards rings III and IV 7,8,10 . A possible explanation of the electron-spin density shift was suggested to be the presence of a local electrostatic field close to ring III, created for example by the protonation of the keto-group of ring V 7 . Since it appears that the electron-spin density on the oxidized donor is also localized on the axial histidine, a tilting of the axial histidine towards pyrrole ring IV causing π-π overlap of both aromatic systems was proposed. In this "hinge model", the charge state of the histidine suggested a negatively charged Chl a-histidine complex becoming a neutral radical in the photo-oxidized state 8,10 (for review, see Najdanova et al. 11 ). Such electronic structures might allow for the remarkable increase in redox potential of PS2 in comparison to bacterial RCs.
To study photosynthetic units larger than D1D2, such as core preparations, BBY membranes or entire plants, selective isotope labeling is required. In this work, we present the first photo-CIDNP MAS NMR data obtained at various levels of PS2 selectively 13 C labeled in the Chl a and Phe a cofactors. The label incorporation succeeded in the aquatic plant duckweed (Spirodella oligorrhiza), which has been chosen since it has shown previously to successfully incorporate 15 N as well as 2 H-, 13 C-or 17 O-labeled tyrosine 12 .

Results and Discussion
Photo-CIDNP MAS NMR spectra obtained from 13C-labeled BBY, thylakoids and plants. In   Fig. 2, Spectra a-c, shown in red, are obtained under continuous illumination of selectively 4-ALA 13 C-isotope labelled BBY (a), thylakoid membranes (b) and from entire plants (c) of the duckweed Spirodella oligorrhiza. The same Figure shows in black Spectra a' to c' obtained from the same samples in the dark. The dark spectra show only weak and broad positive signals in the aliphatic region between 0 and 50 ppm and, due to the C-α of the amino acids of the protein backbone, between 60 and 80 ppm.
The 15 signals that are light-induced by the solid-state photo-CIDNP effect can be straightforwardly recognized. Figure 3 shows an expansion of the spectral region for the light-induced signals in Fig. 2. The data are presented with the tentative assignment (see below) to the labelled carbons in the Chl a donor and Phe a acceptor. For convenience, the isotope label patterns of the Chl a donor (green) and Phe a acceptor (purple, numbering in Italics) obtained by biosynthetic labeling with 4-ALA are indicated with numbering at the red dots on top of Fig. 3. The data show that: (i) The solid-state photo-CIDNP effect allows to observe the two cofactors forming the SCRP directly from entire plants without any further isolation (Fig. 4). This implies that 13 C photo-CIDNP MAS NMR is able to study selectively microscopic structures in the Ångström range within macroscopic units up to the dimension of centimeters. Thus, we propose that such "in-plant" photo-CIDNP MAS NMR could be considered as a new application in a growing field of in-cell solid-state NMR [13][14][15] .
(ii) The comparison of the 13 C photo-CIDNP MAS NMR spectra of BBY particles (spectrum a), thylakoid membrane (spectrum b) and intact leaves (spectrum c) does not reveal any significant difference, neither in the chemical shift nor in the intensity. Minor changes in the relative intensity of the signals might occur at 51.0 and 147.2 ppm, although they are in the limits of the noise. Hence, both, the chemical shift values and the overall intensity pattern are highly conserved among spectra obtained from these three levels of isolation. The consistency observed with the highly sensitive NMR spectroscopy provides clear experimental evidence that the electronic states of the cofactors forming the SCRP are not affected by the preparation procedure. Thus, the data obtained from a D1D2 RC preparation 7,10 indeed reflect the genuine natural state occurring in intact thylakoid membranes and plants.
(iii) The below discussed assignment of the light-induced signals corroborates the concept of a SCRP formed by a single donor Chl a and a single acceptor Phe a. While 13 C photo-CIDNP MAS NMR intensities are correlated  to local electron-spin densities 16 , in case of 13 C labelling, the polarizations are equilibrated by spin-diffusion. On the other hand, the spread of intensity allows to observe nuclei which are otherwise difficult to detect 17 . Spin-diffusion allows for example for the detection of the nearby aliphatic signals for the two C-17 carbons from the donor and acceptor. While in unlabelled samples, all signals are conveniently assigned to a single Chl a cofactor, i.e. the donor 7 , upon 4-ALA 13 C-labelling also acceptor signals are observed. Effects of selective 13 C isotope labelling on the spin-dynamics have also been observed in heliobacterial RCs 18 but are not yet theoretically permeated. The donor signals are in general stronger than the acceptor signals, suggesting an additional contribution of the DR mechanism 19 .

Photo-CIDNP in PS2 preparations containing the OEC.
To allow for the solid-state photo-CIDNP effect, a SCRP on P D1 and Phe a with sufficiently long lifetime, i.e., some 10 s of ns, is required. One might assume that such long lifetime cannot sustain in the presence of the OEC 20 . Our results demonstrate the occurrence of the same SCRP in the preparations of larger PS2 complexes (with the OEC present) with sufficiently long lifetime as in the D1D2 preparation (where the OEC is lost). There are, indeed, arguments for the interruption of the OEC activity in the present set of experiments: (i) The electron transfer from the OEC requires an intact hydrogen bonding network around Tyr Z , the intermediary electron carrier between the OEC and P D1 Chl a 21 . The rate of re-reduction of P D1 + decreases at low pH 22 . This effect has been suggested to be linked to a distortion or breakage of the hydrogen bond between TyrZ and the nearby D1-His190, which has an estimated pK a of 4.5-5.3 [23][24][25] (for review see Styring et al. 26 ). (ii) Below a pH of 5.5, the nanosecond kinetics of electron transfer quickly slows down to the microsecond. The pH dependence provides a natural mechanism for physiological regulation of electron transfer from the OEC, allowing for dissipation of excess excitation energy by the RC at high light conditions 27 . In all experiments shown here, after reduction with Na 2 S 2 O 3 in the rotor, a pH below 5.0 has been reached in preparations of the larger PS2 complexes, i.e., in plants, BBY and core preparations. Furthermore, acidification of the lumen space down to a pH of 5.0 or slightly below occurs in vivo under strong light conditions 28 . Hence, the pH allows for lifetimes of the SCRP sufficiently long to induce the solid-state photo-CIDNP effect. (iii) The temperature of the experiment (~235 K) blocks the re-reduction from the OEC. The reaction cycle of the OEC 29 is strongly inhibited at 230 K [30][31][32] . If we assume that the OEC remains blocked in its S2 state, even at pH 6-7.5, the re-reduction rate would slow down to 250 ns 33 . (iv) Also in core preparations, Q B is lost while Q A remains bound to the protein pocket 34 . In the preparations of larger PS2 complexes, both quinones are, at least initially, present. It is possible that Q B is lost upon reduction prior to the measurement. In this case, after some photocycles, Q B will be saturated and the light-induced electron transfer becomes cyclic. At high light intensities, also under natural conditions, photo-reduction of quinones has been shown to occur leading to double reduction of Q A which finally can result in the release of Q A as Q A H 2 (up to 63% in 80 min) 35 . In core preparations, double reduction of Q A can be significantly promoted by the addition of a strong reductant and subsequent illumination 36,37 . In BBY preparations, illumination under reductive conditions has been demonstrated to cause 100% double reduction of Q A 38 .
Hence, the experimental conditions allow for observation of the SCRP of PS2 despite the OEC being present. 13 C photo-CIDNP MAS NMR on isolated PS2 RCs from spinach and duckweed. As a next step, we will compare PS2 data from spinach and duckweed. The impossibility to obtain D1D2 RCs from duckweed forces us to compare Core particles to D1D2 RC preparations from spinach. In Fig. 5, shown in red, the 13 C photo-CIDNP MAS NMR Spectra a and b are obtained under continuous illumination of a D1D2 preparation from unlabelled spinach (a) and of core particles from unlabelled duckweed (b). Spectra a' and b' , depicted in grey, show the corresponding dark spectra. As expected, the dark spectra show signals in the aliphatic region between 0 and 50 ppm as well as a broad signal between 60 and 80 ppm. The dark signals are due to the C-α of the amino acids of the protein backbone and the (glycine) buffer used for sample preparation. In the photo-CIDNP MAS NMR spectra in Fig. 5, absorptive (positive) and emissive (negative) light-induced signals occur in the region between 80 and 170 ppm. Although the isolated RC shows stronger light-induced features than the Core complex, chemical shifts and intensity patterns between both light-induced spectra are very similar, proving that the PS2 from both organisms function in a similar fashion. The data provide strong evidence that both the electronic ground-state structure as well as the radical-pair structure of the photochemical machinery of PS2 remain unchanged when comparing the two different plant species and upon isolation of D1D2 from the Core complex. This is in line with an optical study using femtosecond transient absorption spectroscopy, which revealed a conservation of the efficient electron transfer rate constants upon isolation of the D1D2 complex from PS2 Core 39 . Also the mechanism of electron transfer, with Chl D1 acting as the primary electron donor and Phe D1 as the primary acceptor, was found to be the same in both systems 39 . Hence, our data demonstrate that the photochemical machinery of PS2 is robust against various states of isolation. The highest level of isolation, the D1D2 RC, is not disturbed, and essentially functioning in the same way as in full plants. Furthermore, we have shown that this machinery is very similar in PS2 of spinach and duckweed.
To compare the spectra of the D1D2 preparation of spinach and that of the Core preparation of duckweed in more details, Fig. 6 provides an enlarged view. Spectrum a, originating from the D1D2 preparation of unlabeled spinach, is reproducing well data from the literature 7,8 . Previously, 23 light-induced signals were observed, most of them were tentatively assigned to the aromatic ring carbons of the Chl a donor. Absence of signal doubling provided a hint for a monomeric donor. The emissive signals between 90 and 130 ppm were identified as the four methine carbons. It has been proposed that the broad emissive response between 140 and 145 ppm and the emissive signal at 129.2 ppm originate from the axial histidine of the Chl a donor 10 . Spectrum b originates from the Core preparation from unlabelled duckweed. Despite the overall great similarity of chemical shifts and other spectral features, the emissive signal between 129 and 130 ppm is apparently missing. Also, the broad emissive signals at 142.5 and 139.8 ppm might be extinguished. All these emissive features are assigned to the axial histidine 8 . MAS rotation might lead to orientation effects in membrane-based samples, changing the relative intensity contribution between π-systems having different orientations 10 . Therefore, the collective absence of these emissivive features in the membrane sample suggests a common origin and backs the assignment to the axial histidine.
Assignment of 13 C photo-CIDNP MAS NMR signals of 5-ALA, 4-ALA and 3-ALA labeled samples. As shown above, the possibility to introduce 13 C isotope labels allows to observe the effect in larger systems. Furthermore, isotope labelling enables for a more detailed characterization of the SCRP and the identification of possible abnormalities. To this end, we will now aim for signal assignment by using selectively 5-ALA, 4-ALA and 3-ALA labeled samples. Since it allows for efficient label introduction, we prepared thylakoid samples from duckweed with various 13 C isotope label patterns to study the structure of the SCRP of PS2. In Fig. 6, Spectra c to e have been obtained from samples were the Chl a and Phe a molecules were specifically 13 C labeled according to the 5-ALA (c), 4-ALA (d) and 3-ALA (e) labeling patterns (for labeling patterns, see Supplementary  Fig. S1). By using specifically labeled samples, it is possible to selectively highlight eight carbons in each active Chl a or Phe a cofactor and assign them by using literature values obtained from Chl a aggregates 40 and plant Phe a reconstituted in bacterial RCs 41 . In Table 1, we aim to reconstruct the spectra of the unlabeled PS2 core and D1D2 samples (Fig. 6, Spectra a and b) by using the data obtained from selectively 13 C-labeled thylakoid membranes (Fig. 6, Spectra c to e). Again, the consistency of many marker lines in labeled and unlabeled samples clearly demonstrates the conservation of the electronic structure of the PS2 Chl a donor upon isolation of core particles from thylakoid membranes. The detailed discussion on the assignment can be found in Supplementary Information. We can conclude from the assignments: (1) While the aromatic system appears largely undisturbed, the absence of the donor C-13 1 carbonyl carbon and the occurrence of the two weak, unassigned so far, absorptive signals at 151.8 and 148.3 ppm in the 3-ALA pattern teases for further studies. (2) The emissive signals at 142.5 and 139.8 ppm, assigned to the axial histidine 10,11 , do indeed not originate from the Chl a and Phe a cofactors. The emissive signal at 129.2 ppm, however, might be caused by the C-12 carbon of the acceptor, although we cannot rule out that that acceptor signal is overlaying the histidine signal. Thus, the matrix is involved into the formation and evolution of the SCRP.
Therefore, below we will test possible chemical modifications of the donor by means of chemical-shift calculations. Possible chemicals modifications 7,8,42 are:  One might assume that the C-13 1 signal has been significantly shifted by a chemical modification at the C-13 1 position. This idea is attractive because it might explain the unchanged optical properties although the electro-chemical properties of these cofactors, esp. the extremely high redox potential, are highly unusual. Possible chemical modifications are protonation and Schiff-base formation at this carbonyl side (see above). To explore the possibility of such chemical modifications, theoretical calculations have been applied. The results of the calculations are listed in Table 2. It can be seen that the calculated shifts are in reasonable agreement with the experimental values for Chl a, with a root-mean-square deviation of 5.3 ppm and a maximum absolute deviation of 10.8 ppm. Changes in these shifts upon chemical modification are expected to be of higher accuracy due to the possibility of error cancellation. This is supported by the observation that these changes are much more consistent with the corresponding changes calculated with the BP86 functional (given in Supplementary Table S1) than the actual Chl a chemical shifts. In all three calculated structures with a chemical modification, the ppm value of carbon C-13 1 lowered. The lowest value, around 160 ppm, is found for the Schiff-base. Therefore, from a spectroscopic view, we would not rule out an assignment of the two possible signals around 150 ppm. However, in the structure 43 , there is no amino acid around the donor Chl a able to form a Schiff base. The two calculated structures with protonated C-13 1 positions are not very likely from the chemical shift values. Hence, a chemical modification as explanation of the absence of the signals of C-13 1 from the donor is unlikely.

Conclusions
(1) The solid-state photo-CIDNP effect can be observed in entire plants. The observation of photo-CIDNP by 13 C MAS NMR directly in plants allows application for exploration of SCRP in systems in which a further isolation has not yet been established. (2) The active photochemical machinery forming the SCRPs of PS2 is conceptually the same as for bacterial RCs, provided the hole transfer on the donor side is blocked by lowering the pH. (3) This machinery remains essentially unaffected upon isolation from plant level to the D1D2 preparation. (4) PS2 RCs in spinach and duckweed show essentially identical 13 C photo-CIDNP MAS NMR spectra demonstrating structural and functional conservation between these plant species. (5) The SCRP is formed by a Chl a donor-Phe a acceptor pair. In both cofactors, the 13 C chemical shifts of the aromatic π-system are close to standard conditions. (6) Based on the comparison of selectively labeled PS2 with natural abundance PS2, involvement of the protein matrix in the formation of SCRP has been demonstrated. It appears that signals not originating from the cofactor can be straightforwardly assigned to a histidine. (7) It does not appear likely that the donor cofactor is chemically modified at the C-13 1 position. Therefore, to explain the unusual properties of the donor, we assume that conformational effects, esp. related to the axial histidine 10 , electrostatic fields 7 and dielectric properties of the protein 44 act together.

Methods
Strains and culture conditions. Spirodella oligorrhiza was grown under aseptic conditions on half-strength Hunter's medium under continuous light (20 μEm −2 s −1 ) at 25 °C. The medium was continuously bubbled with sterile air containing 5% CO 2 . For selective 13 C labeling, fully grown plants were exposed to δ-aminolevulinic acid (ALA, purchased from Cambridge Isotope Laboratories), isotopically 13 C labeled at carbon position 3 (3-ALA), 4 (4-ALA) or 5 (5-ALA) to a final concentration of 1.4 mM in half-strength Hunter's medium at pH 4.8. After 7 days plants were harvested and used directly for sample preparation or frozen in liquid nitrogen and stored at −80 °C until use.
Determination of the 13 C-label incorporation. Chl a was extracted from plants grown in 13 C-ALA supplemented half-strength Hunter's medium (labeled sample) and from unlabeled plants (reference sample), according to the following procedure: Plants were homogenized in half-strength Hunter's medium and centrifuged for 10 min at 16,000 × g. The supernatant was removed and the residue was dissolved in 1 mL MeOH. The methanolic solution was centrifuged for 5 min at 300 × g. The green supernatant was separated from the blue and white residue and dried under a gentle stream of N 2 . The sample was re-suspended in acetone, loaded on a cellulose column and pure Chl a fractions were eluted with petroleum ether/acetone (7/3 v/v). The solvent was evaporated under N 2 flow and the pure Chl a was stored at −20 °C under a dry nitrogen atmosphere in the dark.

Liquid chromatography-mass spectrometry (LC-MS). Mass spectra were measured with a LTQ-FT
hybrid mass spectrometer (Thermo Fisher, Waltham, MA, USA). Spectra were measured in ESI mode, with a source temperature of 200 °C, source voltage of 3.8 kV and tube lens voltage 150 V. Chl a was dissolved in 90% EtOH and 10% 10 mM ammonium acetate to a final concentration of ~1 mg/mL. The sample was infused with a flow rate of 10 μL min −1 . The biosynthetic route from ALA to Chl a and Phe a is described in Schulten et al. 45 . Two molecules of ALA are asymmetrically condensed to form the pyrrole porphobilinogen. Four molecules of porphobilinogen tetramerize, and prior to macrocycle ring closure, the last pyrrole ring is inverted via a spiro-intermediate. Upon incorporation of 3-13 C, 4-13 C or 5-13 C-ALA, a maximum of 8 13 C-atoms can be incorporated into each Chl a or Phe a molecule, resulting into the specific labeling patterns shown in Supplementary  Fig. S1. Based on the LC-MS spectra observed in the region of m/z = 893.5 ([M] •+ ; C 55 H 72 O 5 N 4 Mg) to m/z + 8 (maximum 13 C incorporation) the total level of incorporation (P tot ) was determined via an iterative procedure as described earlier by Schulten et al. 45 . Since ALA is a precursor of both Chl a and Phe a, it is assumed that the level of label incorporation into Phe a and Chl a is identical. Supplementary Fig. S2 shows a typical mass spectrum of Chl a isolated from S. oligorrhiza grown under standard conditions (Fig. S2, A) and in the presence of the 13 C lableled ALA precursor (Fig. S2, B). An average of 75% 13 C isotope enrichment is accomplished. Preparation of D1D2 and Core complex. The natural abundance samples of isolated D1D2 from spinach were prepared as described earlier in Matysik et al. 7 . The PS2 core complexes from duckweed were isolated according to the procedures described by van Leeuwen et al. 6 .
Preparation of 13 C labeled BBY. Selectively 13 C labeled BBY membranes were isolated according to the procedure as described by Berthold et al. 5   rotor. All other samples were previously reduced by the addition of sodium dithionite to a final concentration of 100 mM under oxygen-free atmosphere and low-light conditions and loaded into sapphire rotors. For experiments on BBY, 200 µL of BBY product (Chl a concentration of 1.7 mg/mL) was washed twice with sucrose free BTS-200 buffer (BTS-0) and resuspended in 70 µL of BTS-0 before reduction. For preparation of thylakoid samples, ~200 mg isotope labeled plants were homogenized in a minimal amount of half-strength Hunter's medium at pH 5.8 under near dark conditions. Starch was removed with slow centrifugation at 60 × g for 3 minutes using an Eppendorf 5415D. The supernatant was collected and centrifuged for 10 min at 16000 × g, and the pellet was resuspended in half strength Hunter's medium. For experiments on entire plants ~100 mg plants were incubated in a solution containing 100 mM sodium dithionite for 10 minutes under nitrogen atmosphere in complete darkness. Plants were carefully stacked inside an optically transparent 4-mm NMR sapphire rotor, and half-strength Hunter's medium was added to fill the rotor. In all cases, the filled rotor was directly loaded into the NMR probe and cooled under slow spinning (800 Hz) to 235 K. Membrane and plant samples were always freshly prepared and directly frozen inside the NMR apparatus. The estimated concentration of PS2 RC present at different levels of purification is shown in Supplementary Table S2.
Photo-CIDNP MAS NMR experiments. 13 C-MAS NMR experiments were performed on a DMX-200 NMR spectrometer (Bruker Biospin GmbH, Karlsruhe, Germany). All spectra have been obtained at a sample temperature of 235 K and with a spinning frequency of 8 kHz. The data were collected with a spin echo pulse sequence under two-pulse phase modulation carbon-proton decoupling 46 . Optimized 1 H and 13 C 90° pulse lengths were 5.1 and 3.1 μs, respectively. The cycle delay of 4 s was used, the acquisition time was 35 ms. About 7 k and 20 k scans were used to record continuous illumination spectra of 13 C-labeled and natural abundance samples, respectively. Between 3.5 k and 10 k scans were used to record the respective dark spectra. The 13 C NMR spectra were referenced to the COOH response of solid L-tyrosine hydrochloride at 172.1 ppm. Photo-CIDNP MAS NMR spectra have been obtained under continuous illumination with a 1000-Watt xenon arc lamp 47 .
Spectral fitting. The fitting of the light induced signals obtained by photo-CIDNP MAS NMR has been performed using IgorPro version 6.01 (Lake Oswego, Oregon).

Computational details. Structure optimizations of the compounds [Chl a], [Chl-OH] + , [Chl-OH],
[Chl-NH 2 ] + were carried out with the program package ADF 48 employing the exchange-correlation functional KT2 49 and the triple-zeta TZP basis set 50 . As a consistency check, also calculations with the BP86 functional 51,52 have been performed. The corresponding results are given in the Supporting Information (Table S1). Dispersion interactions were taken into account using the D3 correction by Grimme et al. 53,54 with Becke-Johnson damping [55][56][57] . The numerical quality for the density fit and grid construction procedures were set to "good". Tight convergence criteria were applied during the SCF cycles (1.0e −8 a.u. for the norm of the Fock and density matrices) as well as in geometry optimization (1.0e −4 and 1.0e −4 a.u. for changes in energy and gradient, respectively). The optimized geometries were verified as minima on the potential energy surfaces with normal mode analyses. The same computational settings were used for the generation of potentials required for calculations of 13 C nuclear magnetic shieldings with the NMR module [58][59][60][61][62] . Chemical shifts were calculated with respect to the tetramethylsilane (TMS) shieldings obtained with the same settings as described above.