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Volume Analysis of Periodontal Bone Specimens Retained in Trephine Burs Using Synchrotron Radiation X-Ray Micro-Computed Tomography

Bone regeneration studies require accurate volume measurement to evaluate new bone formation. Periodontal specimens, harvested from bone grafting areas inside the oral cavity using trephine burs (TBs), are conventionally analyzed by bone histomorphometry, which estimates volume fractions from photomicrographs of histological sections. Recent development in non-destructive three-dimensional (3D) imaging analysis using X-ray micro-computed tomography (μCT) shows potential for replacing the conventional method for accurate volume measurement. We further demonstrated that the use of synchrotron radiation X-ray μCT (SR-μCT) can greatly accelerate high quality data acquisition, easing statistical analysis of multiple specimens [1]. One drawback in μCT sample preparation, which can be avoided in bone histomorphometry, is that specimens need to be ejected from TBs for X-ray transmission. During ejection, specimens may get damaged or even break, which is especially detrimental to weak specimens exhibiting poor bone regeneration. Recently, SR-μCT using high photon energy X-rays for higher X-ray transmission is becoming more accessible. In this study, we examined the use of SR-μCT for volume analysis of intact periodontal bone specimens while they are retained in TBs.