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Preparation, characterization and evaluation of a new film based on chitosan, arginine and gold nanoparticle derivatives for wound-healing efficacy

It is well-known that the combination of polymers and nanoparticles (NPs) provides optimised wound dressing and accelerates wound healing. The knowledge about the structure and properties of these materials is of critical importance in biological processes related to wound healing. In this study, we prepared a chitosan (CS) film modified with arginine (Arg) and gold NPs (AuNPs) and investigated its effectiveness as a dressing material for wound healing. Fourier-transform infrared spectroscopy (FTIR) confirmed that Arg was successfully grafted on CS. The resultant CS-Arg/AuNP film was then characterised by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The modification of Arg and AuNPs improved the hydrophilicity, mechanical strength and antibacterial properties of the film, which in turn provided an enhanced ideal environment for cell adhesion and proliferation. Cell Counting Kit-8 (CCK-8) was used to demonstrate the survival rate. Furthermore, the proteins involved in wound healing were evaluated qualitatively and quantitatively by immunofluorescence and western blotting, respectively. The skin defect models used for the in vivo studies revealed that the CS-Arg/AuNP dressing accelerated wound closure, re-epithelialization and collagen deposition. Our cumulative findings support the feasibility of using the proposed film as a promising candidate for tissue engineering of the skin in the near future.


Introduction
Skin-the largest organ and the outermost layer of the human body-protects the body from external injuries, including numerous physical, chemical and microbial agents. Thus, skin defects aer trauma remain a serious threat for the human body. Dermal transplantation is the mainstream treatment approach preferred by many patients. However, skin graing procedures are limited by not only the scarcity of donors and the risks of immune rejection but also by the economic pressure on the patients. Cell therapy is another promising strategy for wound healing using cultured cell gras, but the excessive time duration involved in acquiring adequate amounts of keratinocytes acts as another burden for the patients. 1 In the recent years, wound dressing, as an appropriate strategy for tackling the above-mentioned issues, has been widely studied in the clinical medicine segment, especially in the eld of surgery. Would healing is a complex physiological process that involves blood clotting, wound contraction, vascularization, inammatory cytokine intervention, cell proliferation and nally brous connective tissue formation. 2 Ideal wound dressing materials should thus exhibit the following characteristics that are associated with promoted healing, including good biocompatibility, mechanical strength, water absorption, proliferation promotion, non-toxicity and antibacterial effects. 3 The new skin substitutes should ideally provide appropriate microenvironments for improving vascularization, reducing chemotaxis of inammatory cytokines, enhancing cell proliferation and adhesion and eventually allowing progressive remodelling. Past studies have examined a variety of organic and inorganic materials in wound dressing alone and have achieved good outcomes. [4][5][6] Unfortunately, both these groups of materials have obvious disadvantages, mainly including poor mechanical properties of the organic materials and the weak biocompatibility of inorganic materials. Organicinorganic nanocomposite materials can nevertheless integrate the advantages of the two materials and provide superior properties in terms of physical, chemical and biological properties; this option is currently being widely investigated. 5,7 It is thus important to identify suitable organic and inorganic candidates as dressing materials. In this view, polymers as an organic substance, such as gelatin, polyethylene glycol (PEG), chitin and chitosan (CS), are heralded as promising candidates based on their benecial properties for wound healing.
Considering the origin of biopolymers, CS enjoys unique biocompatibility, biodegradability and non-toxicity, which is undoubtedly a merit in its application for wound healing.
Several studies have demonstrated that CS is applicable in numerous elds related to tissue regeneration. [8][9][10] In addition, CS plays an active role in various stages of wound healing, such as in the cytokine production, macrophages activation and collagen synthesis, which together quicken the healing process. As a positively charged polymer, 11 it attracts growth factors and cytokines near the wound and promotes the deposition of type I collagen at the wound site. Structurally, CS gel has an appropriate porosity, which provides the required conditions to maintain the moisture and air permeability in a wound. 12 Although CS possesses numerous advantages, its application is restricted by its low mechanical strength and insufficient antibacterial properties. Considering these defects, a variety of modied CS alternatives has also been widely studied. 13,14 The wounds are prone to infection, and infected wounds are characterised by progressively increasing volume of exudates, which inhibit the formation of granulation tissue, thereby delaying the healing process. CS has a relatively weak broadspectrum antibacterial property owing to the positive charge carried by the amino groups, which interact with the negative charge on the bacterial surface, causing changes in the cell permeability as well as leakage of intracellular substances. Fortunately, this ability can be enhanced by increasing the number of positive charges, such as by graing onto positively charged amino acids and loading with other antibacterial agents. [15][16][17] Arginine (Arg) is a typical positively charged amino acid. Once CS is modied by Arg, the enhancement of the positive ion group can attract more negatively charged cytokines and growth factors near the wound site and stimulate cell proliferation and differentiation there. Furthermore, Arg is a precursor of nitric oxide (NO) and an active molecule closely related to vascularization, immune response, epidermal cell proliferation and migration and late collagen deposition. Thus, this amino acid plays a signicantly important role in wound healing .18,19 Recently, the use of nanoparticles (NPs) to enhance the physical and chemical properties of biopolymers has attracted the attention of scientists across the world. Among the inorganic materials mentioned earlier, gold NPs (AuNPs) stand out in virtue of their excellent antibacterial performances. Thus, AuNPs are the emerging candidates for repairing defects, especially in the skin. 20,21 Unlike other inorganic materials, AuNPs are chemically inert and size-tunable, and their processing can be controlled by different chemical synthesis methods, which in turn offer the option of changing their physical and chemical properties as desired. In addition, toxicity pertaining to Au is dramatically low when compared with other NPs like silver (Ag), iron oxide (Fe 3 O 4 ) and zinc oxide (ZnO). 22 Moreover, the addition of AuNPs can increase the mechanical strength of CS matrix. The role of AuNPs is not only to protect the wound bed but also to act as a substitute space for the generation of tissues. 23 Finally, surface ligands of NPs can interact with polymer molecules in a multivalent manner to form self-therapeutic agents. 24 Under the coating of CS, the ligand structure of the NPs changes and they get surrounded by positive charges, which not only specically destroy bacterial cell membranes but also inhibit the contact between planktonic bacteria and negatively charged cell surfaces through a strong electrostatic effect.
In the current work, an Arg and AuNPs-modied CS lm was prepared as a synthetic material to provide improved wound dressing for skin repair. While exploring the effect of Arg graing on AuNPs, this paper also explored whether the multivalent manner of AuNPs can offer full play to the advantages of Arg graing. The chemical structure and morphology of the material under study were characterised by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier-transformed infrared spectroscopy (FTIR). Next, the regenerative effect of the skin defects was evaluated by both in vitro and in vivo assays. This study, to the best of our knowledge, is the rst-of-its-kind to investigate the effect of Arg and AuNPs-modied CS lm on skin tissue engineering in a full skin damage condition.

Preparation of CS-L-Arg
Briey, deacetylated CS (0.4 g) was dissolved in 20 mL of 1% (v/v) acetic acid with continuous stirring to achieve a nal concentration of 2% (w/v) CS solution. Then, NHS and EDC were separately added to the CS solution and magnetically stirred until dissolved, followed by the supplementation of L-Arg to the solution. The resultant solution was dialysed for 3 days against distilled water. The nal product was freeze-dried for 24 h and stored until further use.

Preparation of CS-AuNPs and CS-L-Arg/AuNPs
CS-and L-Arg-graed CS were prepared as described in previous Section. HAuCl 4 $3H 2 O was then added at the same molar concentration as CS to distilled water at 75 C for 30 min. Subsequently, sodium citrate was added to the mixture and the mixture was stirred well until a red-coloured solution appeared. Next, CS/AuNPs and CS-Arg/AuNPs were prepared by mixing gold gel solution in the CS and CS-Arg, respectively. The resulting solution was nally lyophilised to obtain a reserve powder.

Production of lms
Three gels were obtained by dissolving CS and 2 other freezedried modied CS powders in distilled water and adding 1% acetic acid to it. These gels were spread uniformly on the well and cross-linked with NaOH for 12 h. Next, the resultant material was disinfected under ultraviolet light for 12 h. Finally, all the materials prepared for the cell experiments were preprocessed in DMED medium for 6 h.

Chemical characterization by FTIR
The Arg coupling and chemical bonds and chemical groups were evaluated by FTIR (VERTEX 70; Bruker).

TEM
To better understand the size of AuNPs, TEM was performed. Briey, the CS-Arg/Au solution was dropped onto a copper grid and the particle distribution was observed under the JEOL 2100 TEM at the room temperature.

Mechanical properties
The universal mechanical testing machine (1121; Instron, UK) was used at a constant cross-head speed to detect the strain of 4 porous samples (30 mm) until the samples were thoroughly broken; at the same time, the values of tensile stress were recorded sequentially.

Hydrophilicity
The contact angle of 4 types of porous samples was measured by a contact angle apparatus (VCA 2000; AST) as the index of hydrophilicity. We xed the samples and kept the surfaces at. Next, 2 mL of double distilled water was carefully dripped onto the sample surfaces. Finally, the water droplets on the lms were photographed and measured by docking the antennae.

Antibacterial activity
To evaluate the antibacterial activity of the lms, antibacterial tests at OD 600 were performed against Staphylococcus aureus and Escherichia coli. Briey, the materials were cut into 20 mm circles and sterilised under ultraviolet light for later use. Both the bacterial species (4.0 Â 10 4 mL À1 ) were cultured in lysogeny broth (LB) media for 2 h at 37 C. Subsequently, 3 mL of the cultured bacteria were incubated with the sterilised material (0.5 g) for 10 h at 37 C. Finally, the absorbances of the bacterial strains from each group were measured with a spectrophotometer at 600 nm to evaluate the antibacterial activities of the porous lms.
Moreover, 5 mL of the bacterial suspension (4.0 Â 10 4 mL À1 ) was prepared in the LB medium. The 4 groups of sterilised materials (0.5 g) were respectively placed in a certain amount of suspension and cultured for 1 h at 37 C. A uorescent dye was then added to the suspension and incubated for 30 min. Finally, the bacterial viability was observed under a uorescence microscope.

Cell proliferation experiment
CCK assays and immunouorescence staining were performed to evaluate the effects of CS-Arg/AuNPs composites on the proliferation and adhesion of Balb/c3T3 cells. According to the different treatment methods employed, the experimental groups were divided into 4 groups: CS, CS/AuNPs, CS-Arg and CS-Arg/AuNPs. 25 Ten thousand cells were added to each well in a 64-well plate, and the survival and proliferation of each group of cells were evaluated by the CCK-8 when the cells adhered to the wall on days 1, 2 and 3. In order to better observe the morphology of cell growth in each group, the samples were observed under the uorescence microscope.

Effects of materials on the protein levels
Immunouorescence and immunoblotting analyses were conducted to quantitatively analyse the effects of materials on the expression of collagen I associated with wound healing in cells. Cell grouping and material processing were performed as described earlier. Aer 3 days of culturing, for immunouorescence, the medium was removed and the cells were xed with 4% paraformaldehyde for 15 min and then washed with PBS thrice, each time for 5 min. The cells were then le to rest at the room temperature for 30 min. The cells were treated with collagen I primary antibody and placed in a wet box for 4 nights. Next, the cells were washed thrice with phosphate-buffered saline (PBS), incubated with a specic secondary antibody at the room temperature for 2 h and washed thrice again with PBS. Finally, the samples were successively processed in 4 0 ,6diamidino-2-phenylindole (DAPI), washed thrice and then observed under the uorescence microscope.
Next, western blotting experiments were performed. Briey, the proteins in the cells were extracted using lyses buffer. Aer the centrifugation step, the proteins were quantied by the BCA Protein Assay Kit, normalised to a uniform concentration by adding the extraction buffer and denatured at 100 C for 5 min. Next, the proteins from each cell group were run through 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis at 125 V and transferred on to a nitrocellulose membrane at 90 V. The membranes were then blocked with 5% non-fat dried milk at 37 C for 1 h and incubated with the following primary antibodies at 4 C: anti-collagen I (rat), anti-VEGF (rat) and antib-actin (rat). Aer overnight incubation with the antibodies, the membranes were washed thrice with Tris-buffered saline-Tween 20 (TBS-T) and further incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (rat). Finally, the proteins were visualised using enhanced chemiluminescence (ECL) detection reagents.

In vivo wound healing assessment
Adult male Sprague-Dawley rats (n ¼ 20, wight: 250 g) were used to evaluate the status of wound healing. The animals were bred in a standard animal room by the same breeder, with ltered water and pellet food readily available. The animals were randomly allocated into 4 groups: Cs, CS/AuNPs, Arg-Cs and Arg-CS/AuNPs (n ¼ 5 per group). 18,26 Each rat was numbered on the tail and raised in different cages. All experimental protocols were approved by the Animal Care and Use Committee of the Jilin University, China and were conducted in accordance with the Guide for the Care and Use of Laboratory Animals of Jilin University and approved by the Animal Ethics Committee of Jilin University. The rats were anesthetised with intraperitoneally injected chloral hydrate. Two days before the experiment, the back hair of the rats was removed using a depilating cream. Four different lms were soaked in alcohol for 24 h to be sterilised under ultraviolet light. Aer the rats were anaesthetised, they were placed in the prone position such that their back skins were fully exposed and then disinfected using iodine. Using a pre-prepared plastic mould, a full-thickness skin defect of 1.5 cm diameter was created on the back and photographed. Each skin defect was covered with a corresponding lm, wrapped with sterile cotton surgical gauzes and xed with breathable bandage. In order to avoid adhesion and contamination, the wound dressings were changed every 3 days aer surgery and photographed. The wound healing rates were measured and evaluated by image analysis soware (Image J). The healing rate was calculated according to the following formula: wound healing rate ¼ [(S 0 ÀS n )/S 0 ] Â 100%, where S 0 is the initial size of the wound and S n is the size of the wound at each observation point.

Histopathological staining
For histological examination of each group, all rats were euthanised on the 12th day of the surgery. The wound and the surrounding skin were completely removed for histopathological staining. The specimens for histopathological staining were xed with formalin, dehydrated with alcohol, cleared with xylene, embedded in paraffin, and then sectioned vertically into 5 mm thick sections. Aer staining with H&E and Masson, histological changes were observed under an optical microscope.

Statistical analysis
Data were analysed using the Origin 8.0 soware (Origin Lab; Los Angeles, CA, USA). Statistical differences between the data were assessed with one-way analysis of variance and post hoc test. Data were expressed as the mean AE standard. Final results with P < 0.05 were considered statistically signicant.

Results and discussion
As shown in Fig. 1, the lms were prepared and their effects on wound healing were explored through cell experiments, bacteriostatic experiments and in vivo animal experiments.

FTIR
The successful graing of Arg onto CS via EDC/NHS coupling chemistry was detected by FTIR. Fig. 2 shows the FTIR spectra of CS and CS-Arg. The peak at 3435 cm À1 was assigned to the characteristic -NH and -OH stretching vibration. Peak near the 1414 cm À1 was assigned to the bending vibration of the singlebond C-H group. In addition, the characteristic peaks present at 1647 and 1563 cm À1 were assigned to the amide I and II bands, respectively. Arg exhibits an absorption band at 1628 cm À1 that was assigned to the guanidine group. The peaks found at 3299 and 3358 cm À1 belong to the stretching vibration peak of -NH 2 , and the peak of 1679 cm À1 is the stretching vibration peak of carboxy carbonyl group. In the FTIR spectra of CH-Arg ( Fig. 2A), the peak at 3441 cm À1 was assigned to the stretching vibration of -NH and -OH, and the peak strength was reduced, which indicates that the content of hydroxyl and amino groups was reduced aer modication. Furthermore, the peak at 1064 cm À1 was attributed to the stretching vibration peak of C-O, which suggests that Arg is indeed linked to the CS backbone. 18,27 Fig. 1 The flow chart depicting the study protocol. Cs-Arg/AuNPs was prepared in the form of film. Next, the wound-healing efficacy and bacteriostatic effect were investigated through in vitro bacterial, Balb/c3T3 cell and vivo animal experiments. Fig. 3A shows the highly magnied TEM image of AuNPs. The particle size and distribution were determined using the image analysis soware (Image J). According to the statistical evaluation, most of the prepared AuNPs were spherical and dispersed with an average size of 10 nm (Fig. 2B and C). Although the toxicity of AuNPs is signicantly lower than that of other metal NPs, size-dependent toxicity has been reported for AuNPs. Recent studies have reported that AuNPs of particle size < 2 nm exhibited higher toxicity as compared to the larger ones. 28,29 In addition, spherical AuNPs of size 10-20 nm exhibited less cytotoxicity and more bacteriostatic properties. Chitosan with a medium molecular weight acts as a better stabilizing agent, 30 which may be related to the size distribution of AuNPs; however, the specic mechanisms in this event remain unclear.

SEM
The microstructure and roughness of the lm play important roles in cellular attachment, migration and proliferation in tissue engineering. SEM images of the surfaces of CS, CS-Arg, CS/AuNPs and CS-Arg/AuNPs are shown in Fig. 2D. The results revealed that the gra of Arg and the modication of AuNPs increased the roughness of the CS lm surface. In particular, some large AuNPs were observed in Au-Cs and Arg-Au-Cs. As reported in previous studies, a certain degree of roughness not only improves the cell affinity but also increases the contact area with the wound and thereby accelerates the haemostatic process. [31][32][33] Therefore, change in the morphology of CS as well as its improved biocompatibility may be due to the modication of Arg and AuNPs.

Mechanical properties
The composite material must have sufficient mechanical strength to better adapt to the changes in the wound surface morphology and structure during the healing process. In addition, prior studies have reported that the superior mechanical strength is benecial not only for cell adhesion but also for wound shaping to accelerate wound recovery. 34 The poor mechanical strength of CS is an important factor that restricts its wide applications in wound healing. 35 However, the gra of Arg and the addition of AuNPs can signicantly improve the mechanical strength of CS. Fig. 3 gives the tensile strength of different lms. The -NH 2 group of Arg combines with the -COOH group of CS to form an amide bond, this intramolecular force may account for the better tensile strength of Arg-modied CS in comparison with that of CS alone. The addition of AuNPs also signicantly improved the tensile strength of CS. Based on previous reports, the use of AuNPs of appropriate size and dispersion can help improve the overall mechanical strength of the resultant nanocomposites. 36,37 Meanwhile, some studies suggest that intermolecular interactions between AuNPs and CS may also contribute to the improvement of the mechanical strength of the nal product. 38 Hydrophilicity Hydrophilicity is another surface property of a material that affects its healing efficiency. Previous studies have shown that a hydrophilic surface is conducive to the adsorption of serum proteins, which is an important prerequisite for cell adhesion and proliferation. [39][40][41] Fig. 4 shows the contact angles of different lms, which is the indicator of hydrophilicity. As can be seen in the gure, the hydrophilicity of CS was enhanced by This journal is © The Royal Society of Chemistry 2020 RSC Adv., 2020, 10, 20886-20899 | 20891 the modication of Arg and AuNPs. The improved hydrophilicity of CS-Arg can be attributed to the presence of hydrophilic groups, including -OH, -NH 2 and -COOH. The addition of AuNPs also improved the hydrophilicity of the composites, and this phenomenon can be likely attributed to the fact that AuNPs reduced the steric effect of cross-linking and resulted in high surface homogeneity. 42 In addition, according to a previous study, Arg and AuNPs can effectively change the surface morphology of CS, and a rough surface can host and disperse more uids-these points may offer further explanation for the improved hydrophilicity of the material. 43 Therefore, as compared with CS alone, CS-Arg/AuNPs can provide a more suitable microenvironment for cell adhesion and proliferation.

Antibacterial activity
Without the integrity, the skin loses its natural barrier against bacteria. Simultaneously, exudate from necrotic tissues can cause bacterial infections, which delay the wound healing process. Therefore, excellent bacteriostatic properties are essential in an ideal dressing material. CS, as a natural cationic polymer, combines with anionic phospholipids on the surface of the bacterial cell via charge effect to achieve antibacterial properties. However, these properties have been reported to have several limitations. 44,45 The gra of Arg can enhance the positive charge of CS and generate the bacteriostatic effect through the charge effect. Furthermore, AuNPs exert antibacterial properties against Gram-negative and Gram-positive bacteria without causing drug resistance. According to most past studies, this mechanism can be attributed mainly to the ability of AuNPs to block the electrostatic ux between the membranes, creating a distortion in the membrane and ultimately in a break-down. 46 Moreover, while killing bacteria, AuNPs produce reactive oxygen species (ROS) that continually destroy the pathogen's cellular components.
The antibacterial properties of the modied CS were evaluated by OD 600 antibacterial tests against S. aureus and E. coli. As shown in Fig. 5, lower optical density was recorded in the bacterial suspension co-cultured with the CS-Arg/AuNPs lm as compared with the suspension co-cultured with other lms. Thus, the antibacterial properties of CS were signicantly improved by the modication of Arg and AuNPs, especially by the latter. In addition, the OD value of the suspension was higher against S. aureus than against E. coli; this nding is consistent with the reports from previous studies.
In addition, the live/dead assay was performed to further conrm the antibacterial effect of the modied CS. Under the uorescence microscope, bacteria with intact cell membranes appeared green, while the dead ones appeared red. The red signals increased signicantly in the CS-Arg and CS/AuNPs groups as compared with that in the CS group; almost no green signals were recorded in the Arg-CS/AuNPs group (Fig. 6).

Cell proliferation experiment
Balb/c3T3 cells are routinely applied, owing to their important role, in the studies of wound repair, including that in the release of growth factors and the formation of myobroblasts to retract the wound. To observe the effects of modied CS on cell survival, the Cell Counting Kit-8 (CCK-8) assays were performed on days 1, 2 and 3; the results of the assays are presented in Fig. 7. Although the presence of CS can reduce the cytotoxicity of AuNPs to some extent, 47 several studies have shown that the cytotoxicity of AuNPs is dose-dependent. Therefore, based on the results of previous experiments, we selected AuNPs at a concentration of 120 ppm. 46,48 On the culture day 1, no statistically signicant differences were noted among the groups. On culture day 3, however, the number of broblasts increased signicantly in both the groups, CS-Arg and CS-Arg/ AuNPs. On culture day 7, broblast survival continued to increase in the other experimental groups when compared with that in the CS and CS/AuNPs groups, the latter was particularly low for the toxicity of AuNPs.
Based on the experimental results, on one hand, although AuNPs exhibited antioxidative effects by effectively quenching  the free radicals like 2,2-diphenyl-l-picrylhydrazyl (DPPH), OH (hydroxyl), H 2 O 2 (hydrogen peroxide), and NO, thereby repairing damaged collagen tissues and reducing cell death, which indicated some degree of cytotoxicity. On the other, cytotoxicity could be signicantly decreased in the presence of CS and CS-Arg. The improved cell compatibility of CS/AuNPs may be due to the coating of CS, which alleviates the cytotoxicity of AuNPs. 47 In addition, the modication of Arg signicantly improved the cellular affinity of CS, which can be explained by the charge effect. Moreover, the changes in the surface morphology and hydrophilicity also improved the cell adhesion and proliferation abilities of the materials.

Effects of materials on protein levels
Fibroblasts achieved epidermal remodelling and vascularization by regulating the expression of related proteins, including collagen I and VEGF. During the wound healing process, broblasts gradually secreted increasing amount of collagen I, which in turn provided suitable conditions for the proliferation of broblasts and epidermal cells, and VEGF promoted the growth of vascular endothelial cells, thereby improving the hypoxic environment of the extracellular matrix (ECM). 49 First, we performed immunouorescence staining to qualitatively observe the change in collagen I in each group. As shown in Fig. 10, the expression levels of collagen I in the CS-Arg and CS-Arg-AuNPs groups were signicantly higher than those in the pure CS group; this observation is consistent with the results of a previous study. 50 In addition, we found that broblasts in CS-Arg and CS-Arg-AuNPs groups had better cytoskeleton and stretch activity relative to that in the CS group, which was also consistent with the results of MTT assays. Therefore, these 2 modication methods are expected to have positive effects on the expression of the related proteins.
Furthermore, western blotting was performed in order to quantitatively observe the expression of collagen I and VEGF in different intervention groups. As shown in Fig. 8, the trend of WB bands conrmed the results of immunouorescence staining, indicating that the Au-CS-Arg group was the most effective one in promoting proteins related to tissue repair. However, the exact mechanism remains unclear. We therefore propose that the positive functions at the protein level are inseparable from the pro-proliferative effect of Arg.

In vivo wound healing assessment
To demonstrate their practical applicability, composites were used as wound dressing materials. As shown in Fig. 9, the general macroscopic photographs of postoperative wound healing at different time points aer wounding were observed and calculated to evaluate the degree of wound contraction among the groups. All the wounds showed inammatory response, while the CS group showed infection and mild oedema; however, no statistical difference was noted in the degree of contraction among the groups on day 3. The wound contraction ratio in the CS, CS/AuNPs, CS-Arg and CS-Arg/ AuNPs groups increased in this order, gradually, until the sacrice on day 12. On day 12, complete re-epithelialization of the wound was noted in the CS-Arg-Au group, while the wounds in the other groups failed to heal completely. The macroscopic appearance and the percent of contraction indicated that the CS-Arg/AuNPs composite greatly accelerated wound healing when compared with the CS, CS-Arg and CS/AuNPs. These excellent characteristics of CS-Arg-Au may be attributed to its remarkable antibacterial property, hydrophilic nature, mechanical strength and biocompatibility.

Histological assessment
The outcomes of wounds and the surrounding tissues shown in Fig. 10 using H&E staining and Masson's trichrome (MT) staining exhibited the histological features of each specimen (CS, CS-Arg, CS-Au and CS-Arg-Au). HE staining showed that the wound size remained large, accompanied by a thin regenerated epithelium and a clearly demarcated subepidermal space in the CS and CS-Au groups. In addition, the tissue defects were surrounded by signicant inammatory cells inltration in the CS group, but only fewer inammatory cells in the CS-Au and CS-Arg-Au groups. This phenomenon explained that AuNPs exhibited anti-inammatory properties, as also proved by past studies. 51 On the contrary, vascularised granulated tissues covered with a thin, uniform and complete epidermis could be observed in the CS-Arg and CS-Arg-Au groups, especially in the latter, while only slight granulation was observed in the CS and CS-Au groups, suggesting that CS-Arg/AuNPs can accelerate the proliferation of broblast and angiogenesis capabilities. At the healing stage, the thickness of the re-epithelialization thickened rst and then gradually thinned until it returned to normal thickness. In addition, new-born hair follicles were observed in the CS-Arg/AuNPs group. The granulation tissue included newly forming blood vessels and collagen bres. Therefore, MT staining was performed for the examination of collagen bres.
Fibroblast synthesised collagen bres is known to form the ECM. Furthermore, the deposition of ECM in turn promotes brosis formation by providing the substrate for broblast Fig. 7 The CCK-8 assays of Balb/c3T3 cells cultured on CS, CS/ AuNPs, CS-Arg and CS-Arg/AuNPs. Error bars represent the mean AE SD (n ¼ 3; * denotes statistically significant difference *p < 0.05). adhesion. Hence, collagen bres play a crucial role in the process of wound healing. When compared with those in the CS-Arg and CS-Arg-Au groups, collagen bres in the CS and CS-Au groups were disorganised and fragmented, which may be because Arg effectively stimulates collagen bres released from broblasts. 52,53 Untidy and loose collagen bres were observed in the CS and CS-Au groups, while well-organised collagen bres were observed in the CS-Arg and CS-Arg/AuNPs groups. In addition, the CS-Au group displayed higher expression of collagen bres than the CS group, which may be attributed to  the antibacterial properties of AuNPs. These results suggest that the CS-Arg/AuNPs treated wound tissues have a considerable amount of collagen deposition and tightly arranged bre network.

Conclusions
In this study, CS-Arg/AuNPs lms were synthesised by modication of CS with Arg and AuNPs; the efficiency of the lms were characterised by FTIR, TEM and SEM. Based on the physical and chemical analyses, the resultant composites were found to exhibit excellent mechanical properties, hydrophilicities and antibacterial activities owing to the presence of chemical bond and metal NPs. In addition, CS-Arg-Au showed a rougher surface compared to the other groups, which was conducive for broblasts to adhere, proliferate and secrete proteins associated with tissue repair mechanisms. Moreover, a series of further in vivo experiments demonstrated that CS-Arg/AuNPs showed a faster speed of wound contraction as well as exhibited a thin layer of re-epithelialization, dense collagen deposition and clearly visible neovascularization in the histological analysis. Overall, our work demonstrated that CS-Arg/AuNPs lm can prevent further infection of a wound to a large extent and reduce the healing time. Therefore, it has potential utility across the eld of wound healing medication and interventions.

Conflicts of interest
There are no conicts to declare.