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Establishment of fingerprint and mechanism of anti-myocardial ischemic effect of Syringa pinnatifolia.

OBJECTIVE
To establish the fingerprint of Syringa pinnatifolia Hemsl. (SP), analyze the blood components of SP, and explore the possible mechanism of SP's anti-myocardial ischemia, so as to provide scientific basis for the follow-up development and research of SP and lay a foundation for its clinical application.


METHODS
The fingerprint of SP was established by UPLC-QE-MS and GC-MS. A rat Myocardial infarction (MI) was constructed by ligating the left anterior descending branch (LAD) of the rat coronary artery, and SP alcohol extract was administered to evaluate its anti-myocardial ischemic effect. We analyzed the blood components of SP, screened the active compounds, established a database of SP anti-myocardial ischemic targets, and explored the possible mechanism of SP in treating MI by bioinformatics. The rats were examined by echocardiography, serum biomarkers were determined, and pathological changes were observed by histopathological examination. TUNEL staining was performed to detect the apoptotic level of cells, and western blot and qRT-PCR were performed to detect the expression levels of Bcl-2, Bax and caspase-3 in heart tissues.


RESULTS
In the fingerprint of SP, 24 common peaks were established, and the similarity evaluation results of 10 batches of SP were all > 0.9. UPLC-QE-MS and GC-MS detected a total of 17 active ingredients in the drug-containing serum, including terpenoids, flavonoids, phenols, phenylpropanoids and phenolic acids, the most abundant of which was resveratrol. Enrichment analysis of SP targets against myocardial ischemia revealed that key candidate targets of SP were significantly enriched in multiple pathways associated with apoptosis. Resveratrol was administered to the successfully modeled rats, and the results showed that the resveratrol group significantly reduced LVEDd and LVEDs and significantly increased EF and FS in all groups compared with the model group. Resveratrol significantly reduced the levels of CK-MB and LDH in serum compared to the model group (p < 0.001). Hematoxylin-eosin (HE) staining of rat myocardial tissue showed that all lesions were reduced under microscopic observation in the resveratrol group compared with the model group. RT-PCR and western blot results showed that resveratrol group down-regulated the expression of the pro-apoptotic factor Bax, up-regulated the expression of the anti-apoptotic factor Bcl-2, and decreased the expression of Caspase-3.


CONCLUSION
The established fingerprints are accurate, reliable and reproducible, and can be used as an effective method for the quality control of the herbs. The anti-myocardial ischemia effect of SP may be that resveratrol can improve cardiac function and inhibit cardiomyocyte apoptosis to protect cardiomyocytes. The present study provides ample evidence for the clinical use of SP, suggesting that this drug has great potential in the treatment of ischemic heart disease.