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Short Papers in Pharmaceutical Science

The gene for a model protein thioredoxin (Trx) was produced as a ybbR-Trx fusion protein using standard molecular biology techniques. Bioresistant photoreactive siloxane monolayers were prepared and patterned by exposure to 325-nm laser light. CoA was subsequently attached to the exposed areas via a suitable maleimide linker molecule. The ybbR-protein was immobilised by attachment to the surface CoA under Sfp as catalyst (Figure 1). The surfaces were then imaged by atomic force microscopy (AFM).