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Similar Effects of Treatment with α Interferon on the Protein Synthesis of Human Large Granular Lymphocytes, T Cells, and Monocytes

Preparations of human large granular lymphocytes (LGL), T cells, and monocytes (MC) were obtained through centrifugation on Percoll gradients and preparative E‐rosetting. The different preparations contained more than 80% of the appropriate cell type, as judged by their ability to lyse 51Cr‐labelled K562 cells, cell morphology, and the presence of cell surface structures recognized by the OKT3, OKT10, Leu 7 and OKM1 monoclonal antibodies. The protein synthesis in unstimulated and α interferon (IFN‐α)‐treated cells of the different types was studied by subjecting 35S‐methionine‐labelled cell extracts to two‐dimensional gel electrophoresis. The general pattern of protein synthesis in LGL and T cells was virtually identical, whereas at least 7 major proteins were synthesized at a higher rate in monocytes. The effects of IFN‐α on the protein synthesis of LGL and T cells were identical. IFN‐α increasing the rate of synthesis of 9 proteins. These proteins were also expressed, but not always IFN‐augmentable, in monocytes. No additional, cell‐type associated, IFN‐inducible proteins were found. This suggests that the augmenting effect of IFN‐α on the cytotoxic capacity of LGL, T cells, and monocytes may be to affect common steps in their lytic machineries.