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AN OPTIMAL CONTROL PROBLEM FOR CRYOPRESERVATION OF CELLS USING ULTRARAPID FREEZING TECHNIQUE

An optimal control algorithm for cryopreservation of cells using ultrarapid freezing technique is applied successfully in the present study to determine the strength of optimal laser heating based on the desired limited temperature distribution of the cell. The validity of this optimal control analysis utilizing the conjugate gradient method of minimization is examined using numerical experiments. Three different heating times are given, and the corresponding optimal control heat fluxes are determined. Results show that the optimal boundary heat fluxes can be obtained with any arbitrary initial guesses within a very short CPU time on a Pentium III 600-MHz PC.