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A Novel PCR-RFLP Genotyping of Flavobacterium psychrophilum Targeting the gyrB Region

― A novel PCR-RFLP typing method was developed using PCR primers speciﬁc for the B subunit of DNA gyrase gene ( gyrB ) of Flavobacterium psychrophilum and restriction endonuclease Bsp 119I. With this PCR-RFLP, 300 isolates of F. psychrophilum were classiﬁed into two genotypes (197 and 103 isolates), and the respective genotypes were correlated with the host ﬁsh species from which the isolates were derived. These isolates were further subdivided into 12 genotypes by combining with three previous PCR-RFLPs. It is suggested that this PCR-RFLP typing system can be a useful tool in epizootiological studies on bacterial cold-water disease.