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The effect of specific cell inactivation on the cellular immune response to ferreodoxin peptides

Previous studies using synthetic immunogenic molecules containing two haptenic peptides (the NH2‐terminal heptapeptide and the COOH‐terminal pentapeptide of oxidized ferredoxin (O‐Fd) from C. pasteurinaum ) have shown that both peptides individually are capable of initiating lymphocyte transformation and inhibiting migration in populations of lymphocytes from O‐Fd‐sensitized guinea pigs. While migration inhibition could readily be stimualted by single haptenic peptides, lymphocytes transforamtion appeared to be more easily induced by molecules containing two or more haptenic peptides (these could be identical or different) (Kelly, B., Levy, J. G. and Hull, D., Eur. J. Immunol., 1973. 3: 574). If lymphocyte transformation is a T cell‐mediated phenomenon, these observations indicate the possibility of T cell‐T cell interaction. The two haptenic peptides (designated „N”︁ and „C”︁) were synthesized and conjugated to succinylated bovine serum albumin (S‐BSA), forming the cojugates N‐S‐BSA and C‐S‐BSA, repectively. These conjugates were labled to high specific activity with 125iodine and were used in and „antigen suicide”︁ procedure to treat guinea pig lymph node cell preparation previously sensitized to 0‐Fd adn keyhole limpet hemocyanin (KLH). Cell population exposed to either 125I‐labeled N‐S‐BSA or 125I‐labeled C‐S‐BCA demonstrated decreased lymphocytes transformation in the presence of O‐Fd but not in hte presence of KLH. These results indicate specific cell inactivation cell transformation. Experiments performed by mixing 125I‐labeled N‐S‐BSA‐treated cells with 125I‐labeled C‐S‐BSA‐treated cells were successful in partially restoring the response to O‐Fd and suggest possible synergy between N and C determinant binding cells in the cellular immune response to O‐Fd. Evidence from B cell depletion studies suggests that this is a T cell‐T cell interaction.