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A new method for the detection and elimination of Hydrangea ringspot virus

Summary The complete eradication of Hydrangea ringspot virus (HRSV) from Hydrangea by in vitro pre-treatment with ribavirin and meristem tip culture is reported. A sensitive RT-PCR method for the detection of HRSV in newly-formed regenerants was developed. Total nucleic acid extracts from HRSV-infected Hydrangea leaves diluted 105-fold gave a positive reaction using this method.