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[The Content Determination of Biotransformation of Rb1 in the Total Saponins of Panax notoginseng by A Plant Endophyte Coniochaeta sp.].

Objective
To investigate the specific biotransformation product of ginsenoside Rb1 of Panax notoginseng saponins( PNS) by an individual plant endophyte.


Methods
The endophytes of an invasive plant were selected as the screening targets of active conversion strains. The chromatography and high performance liquid chromatography were used to detemine the metabolite.


Results
Totally, an active strain of conversion of ginsenoside Rb1 were achieved. The strain can specifically convert ginsenoside Rb1 of PNS to ginsenoside Rd and rare saponin ginsenoside C-K,with the conversion rate of 11. 62% of ginsenoside C-K by fermentation for 12 d.


Conclusion
This transformation can obviously increase the content of minor ginsenoside C-K in PNS,and it is expected to be a new way to obtain the active saponin ginsenoside C-K in quantity.