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Green Biosynthesis of Iron Oxide Nanoparticles and Testing Their Inhibitory Efficacy Against Some Pathogens

The biosynthesis of iron oxide (Fe2O3, also known as haematite) nano particles (NPs) using Hydra helix and Beta vulgaris aqueous extracts were adduced, respectively, where the extracts act as a stabiliser and reductant reagent. The crystal structure and size of particles were investigated using X-ray diffraction (XRD), while the morphology was examined using field emission scanning electron microscopy (FESEM), XRD patterns showed the synthesised nanoparticles with well-crystallised structure from Beta vulgaris extract with size 12 nm, while the results by using Hydra helix showed many peaks back to Goethite phase with 16 nm. The antibacterial and antifungal activity were examined using Staphylococcus (showed inhibition zone diameter 23 mm, 16 mm using Hydra helix and Beta vulgaris, respectively), E. coli (showed no inhibition) and Candida fungi (showed inhibition zone 16 mm, 11 mm using Hydra helix and Beta vulgaris, respectively).