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A microfluidic assembly line for adherent cell assays

We report on a novel system for the analysis of adherent cells. Using standard lithography techniques, cell pallets are fabricated upon which cells are cultured and subsequently transported through a microfluidic system. This technology eliminates the need for the release of a cell from its growth surface, which can lead to membrance damage, and/or disruption of normal cell functions. We have demonstrated RBL (rat basophil leukemia) cells will grow on SU8 pallets and remain viable after the pallet is released. Cells on pallets can then be sorted based on pre-selected criteria and sent to a docking station for cell assays. Once completed, the cells may be interrogated and retained for further processing or disposal.