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[High-performance capillary electrophoresis for determination of urinary citrate and oxalate].

OBJECTIVE
To develop a method for determining urinary citrate and oxalate using high-performance capillary electrophoresis.


METHODS
Capillary electrophoresis with indirect ultraviolet (UV) detection was performed for determining urinary citrate and oxalate. Sodium hromate was used as the UV-absorbing background electrolyte.


RESULTS
Citrate and oxalate had a migration time of 3.76 min and 3.14 min respectively. The calibration curves were linear within a limit of about 2 microg/ml for citrate and about 1 microg/ml for oxalate. The recovery rates of citrate and oxalate were 97.53% and 99.11% respectively.


CONCLUSION
This method is simple, accurate and at low cost for determining citrate and oxalate in the urine.