{'fraction_non_alphanumeric': 0.028361344537815126, 'fraction_numerical': 0.005777310924369748, 'mean_word_length': 5.9021739130434785, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Odour enrichment increases adult‐born dopaminergic neurons in the mouse olfactory bulb

The olfactory bulb (OB) is the first brain region involved in the processing of olfactory information. In adult mice, the OB is highly plastic, undergoing cellular/molecular dynamic changes that are modulated by sensory experience. Odour deprivation induces down‐regulation of tyrosine hydroxylase (TH) expression in OB dopaminergic interneurons located in the glomerular layer (GL), resulting in decreased dopamine in the OB. Although the effect of sensory deprivation is well established, little is known about the influence of odour enrichment on dopaminergic cells. Here we report that prolonged odour enrichment on C57BL/6J strain mice selectively increases TH‐immunopositive cells in the GL by nearly 20%. Following odour enrichment on TH–green fluorescent protein (GFP) transgenic mice, in which GFP identified both mature TH‐positive cells and putative immature dopaminergic cells expressing TH mRNA but not TH protein, we found a similar 20% increase in GFP‐expressing cells, with no changes in the ratio between TH‐positive and TH‐negative cells. These data suggest that enriched conditions induce an expansion in the whole dopaminergic lineage. Accordingly, by using 5‐bromo‐2‐deoxyuridine injections to label adult‐generated cells in the GL of TH–GFP mice, we found an increase in the percentage of 5‐bromo‐2‐deoxyuridine‐positive dopaminergic cells in enriched compared with control conditions, whereas no differences were found for calretinin‐ and calbindin‐positive subtypes. Strikingly, the fraction of newborn cells among the dopaminergic population doubled in enriched conditions. On the whole, our results demonstrate that odour enrichment drives increased integration of adult‐generated dopaminergic cells that could be critical to adapt the OB circuits to the environmental incoming information.