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Activation Capacities Different Migrating Behaviors and T Cell and CD14 Dendritic Cells Presenting Transcriptomically Related to Human CD1a Pig Skin Includes Dendritic Cell Subsets

Swine skin is one of the best structural models for human skin, widely used to probe drug transcutaneous passage and to test new skin vaccination devices. However, little isknown aboutitscomposition inimmune cells,and amongthemdendriticcells(DC),that areessential in the initiation of the immune response. After a ﬁrst seminal work describing four different DC subpopulations in pig skin, we hereafter deepen the characterization of these cells, showing the similarities between swine DC subsets and their human counterparts. Using comparative transcriptomic study, classical phenotyping as well as invivo and invitro functional studies, we show that swine CD163 pos dermal DC (DDC) are transcriptomically similar to the human CD14 pos DDC. CD163 pos DDC are recruited in inﬂamed skin, they migrate in inﬂamed lymph but they are not attracted toward CCL21, and they modestly activate allogeneic CD8 T cells. We also show that CD163 low DDC are transcriptomically similar to the human CD1a pos DDC. CD163 low DDC migrate toward CCL21, they activate allogeneic CD8 and CD4 T cells and, like their potential human lung counterpart, they skew CD4 T cells toward a Th17 proﬁle. We thus conclude that swine skin is a relevant model for human skin vaccination. The Journal of Immunology , 2014, 193: