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A Novel Bleached Fluorescence Imaging Technique for Microfluidics


 A bleached fluorescence imaging technique for visualizing microscale flow fields and obtaining scalar diffusion and convection information has been developed. In order to make the technique quantitative, experiments have been conducted to measure the electrophoretic mobilities of fluorescein and fluorescein-dextran conjugates as well as the diffusion coefficients of a fluorescein-dextran conjugate. The technique offers the ability to track undisturbed fluorophores and to use fluorescent conjugates already present in the system. Typical polymer and glass microfluidic substrates will not significantly attenuate the laser light illumination as only visible light wavelengths are required. Applications of the technique to electroosmotically and pressure-driven flows are presented.