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Identification of Specific Protein Markers That Correlate with Initiation and Progression of Microbially Influenced Corrosion.

Abstract : Attempts to resolve proteins in extracts of pipeline pigging solids into its individual protein components by high resolution two dimensional polyacrylamide gel electrophoresis (2-D PAGE) have so far, been unsuccessful. Problems have been encountered during isoelectric focusing in the first dimension. Under the conditions employed, no resolution of the proteins into individual components was obtained. This could be attributed to presence of interfering ions in the samples, inappropriate ampholyte composition or inadequate sample solubilization conditions. Appropriate steps will be taken to correct these problems and optimize conditions for 2-D PAGE. This will include modifications in sample preparation procedures to remove potential interfering ions in isoelectric focusing as well as modifications in gel and sample buffer formulations.