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Deletion of CTNNB1 in inhibitory circuitry contributes to autism-associated behavioral defects

Mutations in β-catenin (CTNNB1) have been implicated in cancer and mental disorders. Recently, loss-of-function mutations of CTNNB1 were linked to intellectual disability (ID), and rare mutations were identified in patients with autism spectrum disorder (ASD). As a key regulator of the canonical Wnt pathway, CTNNB1 plays an essential role in neurodevelopment. However, the function of CTNNB1 in specific neuronal subtypes is unclear. To understand how CTNNB1 deficiency contributes to ASD, we generated CTNNB1 conditional knockout (cKO) mice in parvalbumin interneurons. The cKO mice had increased anxiety, but had no overall change in motor function. Interestingly, CTNNB1 cKO in PV-interneurons significantly impaired object recognition and social interactions and elevated repetitive behaviors, which mimic the core symptoms of patients with ASD. Surprisingly, deleting CTNNB1 in parvalbumin-interneurons enhanced spatial memory. To determine the effect of CTNNB1 KO in overall neuronal activity, we found that c-Fos was significantly reduced in the cortex, but not in the dentate gyrus and the amygdala. Our findings revealed a cell type-specific role of CTNNB1 gene in regulation of cognitive and autistic-like behaviors. Thus, this study has important implications for development of therapies for ASDs carrying the CTNNB1 mutation or other ASDs that are associated with mutations in the Wnt pathway. In addition, our study contributes to a broader understanding of the regulation of the inhibitory circuitry.


Introduction
Autism spectrum disorder (ASD) is a class of neurodevelopmental disorders characterized by persistent deficits in social communication and social interaction (1). Patients with ASD often have an increased risk of anxiety (2) and memory deficits (3). The prevalence of ASD is on the rise, and no effective treatments exist. Although the etiology is not completely known, many genetic factors are associated with ASDs (4). Both structural chromosomal changes (chromosomal translocation and copy number variations) and single-gene mutations lead to ASD-related clinical manifestations (5). Recent exome sequencing revealed that many de novo nonsense mutations † These authors contributed equally to this work. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com significantly increase the risk for ASD. Intriguingly, de novo mutations are enriched in genes that participate in the Wnt pathway, suggesting that the Wnt signaling pathway represents a convergence of the genetic risks in ASD (6). CTNNB1 is a fundamental component of the canonical Wnt signaling pathway and controls cell growth and cell adhesion (7,8). Consistently, nonsense and missense mutations in CTNNB1 were identified in patients with ASD (6) and intellectual disability (ID) (9). CTNNB1 directly interacts with multiple top ASD risk genes within the ASD genetic network (6). That analysis implicated CTNNB1 as an important modulator among 22 ASD risk genes.
Dysregulation of CTNNB1 leads to abnormal brain development (10)(11)(12) and defective dendritic morphogenesis (13,14). By interacting with N-cadherin, CTNNB1 shapes synaptic structure (15,16) and regulates excitatory postsynaptic strength (17,18). In addition, the axonal localization and translation of CTNNB1 modulate presynaptic vesicle release (19)(20)(21). As a consequence, abnormal levels of CTNNB1 in different brain regions or circuits lead to impaired memory (22)(23)(24), and a depression-like phenotype (25). Consistent with the hypothesis that the Wnt signaling is a convergent pathway for ASD, several mouse models with deletions in genes involved in the Wnt pathway show deficiencies in social interaction and repeteative behaviors (26)(27)(28). However, the circuitry mechanisms for how CTNNB1 dysfunction leads to the pathogenesis of ASD are largely unknown.
An imbalance of excitatory and inhibitory signals has been implicated in the pathophysiology of ASD. As a major type of GABAergic interneurons, parvalbumin-positive (PVþ) interneurons generate fast-spiking inhibitory output (29). Cell densities of GABAergic interneurons, including PV-, calbindin-, and calretinin-positive neurons, are changed in postmortem brains of autism patients (30,31). GAD67 and GAD65, the enzymes required for GABA synthesis, are altered in autism (32,33), and PV neuron abnormality was found in different mouse models of autism (34,35). Particularly, PV knockout mice have behavioral phenotypes similar to all three core symptoms in human ASD patients (36), suggesting PV interneurons represent a major neuron type that mediates ASD pathophysiology. Interneuron deficits have been reported in several psychiatric disorders, including schizophrenia and ASDs (37).
The Wnt signaling pathway is required for proper interneuron development. However, the behavioral function of CTNNB1 in PV interneurons has not been identified. Here we established a CTNNB1 conditional knockout (cKO) mouse specifically in PV interneurons and showed that CTNNB1 cKO mice have increased anxiety, impaired cognition and social interactions, and elevated repetitive behaviors, which mimic some core symptoms of patients with ASD. Interestingly, CTNNB1 deletion in PV-interneurons enhances spatial memory and alters the neuronal activity in the cortex.

CTNNB1 knockout changes the distribution of PV þ interneurons
To gain new insight into CTNNB1's functions in inhibitory neural circuits, we selectively eliminated CTNNB1 by Credependent deletion of loxP flanked exons 2 and 6, which leads to an unstable protein (12). CTNNB1 flox/flox mice were mated with PV-Cre mice to generate mice with CTNNB1 cKO in PV interneurons. CTNNB1 flox/flox mice were used as controls because they express normal levels of CTNNB1 (12). Mice with genotypes of CTNNB1 flox/flox and PV-Cre; CTNNB1 flox/flox were selected for behavior tests. All mice tested were born within 5 days, and behavioral tests started after postnatal day 60 (P60). Immunofluorescent staining confirmed that CTNNB1 was deleted from most PVþ neurons (Fig. 1A). A few PVþ neurons still expressed CTNNB1 probably due to incomplete recombination by cre recombinase. As PV is expressed postnatally (38), we further tested how deletion of CTNNB1 affects the distribution of PV interneurons. Intriguingly, the density of PVþ neurons in the prefrontal cortex was significantly higher in CTNNB1 cKO mice (Fig. 1B). However, the percentages of PVþ neurons in different hippocampal regions, the amygdala, and the hypothalamus were not changed ( Fig. 1C and D), suggesting the alteration is region-specific. To gain more insights on the developmental distribution of PVþ neurons, we found that at an early developmental time, postnatal day (P22), the localization of PVþ interneurons was not changed by CTNNB1 cKO in the cortex (Supplementary Material, Fig. S1A and B) or the hippocampus (Supplementary Material, Fig. S1C), suggesting that mislocalization of PV cells occurs between P22 and P60.

CTNNB1 cKO in PV interneurons increases anxiety but not depression-related behavior
To determine if CTNNB1 cKO in PV interneurons affects any motor function, we examined them in the open field test (OFT). PV-Cre; CTNNB1 flox/flox mice and controls had no differences in total distance travelled ( Fig. 2A) Fig. S2D). In addition, the rotarod test showed no significant difference between the groups (Supplementary Material, Fig. S3). These data demonstrated that PV-Cre; CTNNB1 flox/flox mice had no deficits in motor coordination and locomotor function. However, significant differences were detected on center duration time, center distance traveled, frequency and latency of center entry. In the OFT, PV-Cre; CTNNB1 flox/flox mice had significantly less center distance traveled (P ¼ 0.01) (Fig. 2B), with similar velocities (P ¼ 0.76) (Fig. 2C). In the 5-min test, PV-Cre; CTNNB1 flox/flox mice spent less time in the center region (P ¼ 0. 03) (Fig. 2D). PV-Cre; CTNNB1 flox/flox mice showed less center entry (P ¼ 0.02) (Fig. 2E) and a longer latency to enter the center region (P ¼ 0.005) (Fig. 2F). The evidence from of OFT showed that mice with CTNNB1 cKO in PV neurons avoid traveling in the center region (Fig. 2G), suggesting an increase of anxiety in CTNNB1 cKO mice.
To confirm the elevated anxiety-like behaviors in CTNNB1 cKO mice, we used the elevated plus maze (EPM), a wellestablished test for assessing anxiety responses in rodents (39). PV-Cre; CTNNB1 flox/flox mice showed a significant difference on the latency of arms entry compared to littermate controls. It took much longer (P ¼ 0.048) for CTNNB1 cKO mice to enter open arms (Fig. 3A). They entered the closed arms much earlier (P ¼ 0.002) than the CTNNB1 flox/flox mice (Fig. 3A), suggesting that cKO mice are more anxious. CTNNB1 flox/flox mice showed a similar frequency entering either open or closed arms (Fig. 3B). However, PV-Cre; CTNNB1 flox/flox mice preferred the closed arms more often than the open arms (P ¼ 0.0007), and they entered the closed arms more frequently than the control mice (P ¼ 0.014) (Fig. 3B). Although two groups traveled similar total distance in closed and open arms (  Fig. S4C). Head-dipping is a compulsive-like behavior that is a high risk for anxiety (39,40).
PV-Cre; CTNNB1 flox/flox mice significantly increased their headdipping behavior (P ¼ 0.047), especially at the center region of the plus maze apparatus (P < 0.001) (Fig. 3D). Rearing behavior did not significantly increase in our test (Supplementary Material, Fig. S4D). It may be that PV-Cre; CTNNB1 flox/flox mice traveled less in the open arms. Our results from the OFT and EPM indicated that CTNNB1 KO in PV þ neurons leads to an increase of anxiety behaviors. The tail suspension test assesses the efficacy of antidepressant drugs and determines depression-related behavior in genetic mouse models (41). In this test, the depressionrelated behavior of our mice was not affected (Supplementary Material, Fig. S5). The immobile time (lack of struggle-related behavior) was not significantly altered in CTNNB1 cKO mice (P ¼ 0.237).

CTNNB1 cKO in PV neurons alters recognition performance
Because mutations in CTNNB1 result in IDs (9) and PV interneurons modulate cognitive functions (42), we tested how CTNNB1 defects in PV interneurons alter cognitive function and memory.
Using the novel object recognition (NOR) test, mice were exposed to different objects after acclimation to a pair of identical objects. No significant difference was found between CTNNB1 cKO mice and control littermates when interacting with two identical objects (Supplementary Material, Fig. S6A and B), suggesting that the presence of objects did not alter their exploratory behaviors. One hour after acclimation, one familiar object was replaced with a novel object. Both groups of mice showed increased interest in the novel object. Both interactive duration and frequency of novel object were higher than with the familiar object ( Fig. 4A and B). These data suggest that CTNNB1 cKO mice maintain normal short-term memory (1 h). After 24 h, the animals were retested when novel object 1 was paired with novel object 2. The control mice spent significantly more time with the novel object 2 (P ¼ 0.028) (Fig. 4C), suggesting that they  Fig. S6C). The NOR test indicated that CTNNB1 cKO from PV þ neurons affected the long-term recognition but not the short-term memory.
PV interneurons synchronize spiking activity in neuronal networks and modulate fear memory (43,44). Because long-term recognition memory was changed in CTNNB1 cKO mice, we examined fear memory with a fear conditioning and extinction paradigm. No significant difference was detected in the contextual fear conditioning test during the first 2 days, suggesting that the CTNNB1 cKO mice had an intact fear memory. Interestingly, CTNNB1 cKO mice retained a high freezing percentage, and control littermates showed significant reduced extinction formation on day 3 (Fig. 4D). These data indicate that CTNNB1 cKO in PVþ neurons did not change the contextual fear condition. However, memory extinction was impaired as new memories cannot be formed in CTNNB1 cKO mice.

PV-Cre; CTNNB1 flox/flox mice exhibits enhanced spatial memory
Several autistic mouse models showed abnormal spatial memory (45)(46)(47)(48)(49). We used the Morris water maze (MWM) assay to measure spatial memory. Within 4 training days, PV-Cre; CTNNB1 flox/flox mice took less time (Fig. 5A) and swam shorter distances (Fig. 5B) to reach the platform. They also showed a higher percentage of successful rate of finding the hiddenplatform within 60 s (Fig. 5C). On the testing day, after the platform was removed, we measured the time spent in the four quadrants, based on the position of the platform. PV-Cre; CTNNB1 flox/flox mice showed decreased travel distance (P ¼ 0.003) (Fig. 5D), longer duration (P ¼ 0.03) (Fig. 5F) and greater travel frequency (P ¼ 0.02) (Fig. 5G) into the platform quadrant than control littermates. Furthermore, to better quantify spatial memory, we used a three-zone assessment with concentric circles of different diameters surrounding the platform (Fig. 5E). Interestingly, PV-Cre; CTNNB1 flox/flox mice showed better navigation to the target. They had significantly shorter latencies to reach the smallest target zone (P ¼ 0.02), spent significantly longer time (P ¼ 0.02), and entered more (P ¼ 0.01) in the target zones ( Fig. 5H-J). These indicate that PV-Cre; CTNNB1 flox/flox mice can memorize the position of the platform in relation to spatial cues with higher accuracy.

CTNNB1 cKO in PV neurons alters social behaviors and increased repetitive behaviors
ASD is characterized by social-interaction difficulties and repetitive behaviors. The social interaction test (SIT) uses a threechamber paradigm to examine social memory and social novelty in mice. Both groups of mice showed no preference for empty chambers during the habituation (Supplementary Material, Fig. S7A). When a mouse is presented with an empty chamber, both control (P < 0.001) and PV-Cre; CTNNB1 flox/flox mice (P < 0.01) preferred to interact more with the mouse rather than the empty chamber ( Fig. 6A and B), suggesting that both groups of mice maintain normal social motivation. In the second session, an unfamiliar mouse, was introduced in the empty chamber. The CTNNB1 flox/flox mice preferred to interact with the unfamiliar mouse with significant higher interacting time (P < 0. 001) and frequency (P ¼ 0.015) (Fig. 6C-E). In contrast, PV-Cre; CTNNB1 flox/flox mice failed to show social novelty behavior in this session.
Another core symptom of ASD is repetitive behavior. To determine if CTNNB1 cKO in PVþ neurons leads to changes of repetitive behavior, we measured the total grooming time in a testing session of 25 min. CTNNB1 cKO mice had significantly more grooming time than their control littermates (P ¼ 0.033) (Fig. 7A). The marble burying test (MBT) has been used to study stereotypic behaviors related to obsessive-compulsive disorder (50), which also reflects a repetitive and perseverative behavior in ASD patients. PV-Cre; CTNNB1 flox/flox mice took more time to start to bury the marbles (P ¼ 0.046) (Fig. 7B). This result may also reflect increased anxiety in CTNNB1 cKO mice. Yet the final number of buried marbles was not statistically different in the two groups (P ¼ 0.758) (Supplementary Material, Fig. S8). Thus, CTNNB1 cKO mice dug more frequently in a shorter active period. Some autism mouse models also showed problems with nest building (51). Nesting has been reported to be related with defects in the septum or the hippocampus (52,53). PV-Cre; CTNNB1 flox/flox mice performed worse on nest building test than control mice at different times. The scores were significant lower at 1 (P ¼ 0.02) or 24 (P ¼ 0.04) h after setting up the nesting (Fig. 7C). Moreover, we have tested the effect of gender in several behavioral tests (Supplementary Material, Figs. S2E-H, S4E-G and S7B). There is no significant difference between females and males with the same genotype. Between different genotypes, both males and female went the same direction in several tests. Thus, we believe that it will not affect our interpretation when grouping them together. Our data suggest that CTNNB1 KO in PVþ neurons alter the neuronal network and lead to autistic-like behaviors.

CTNNB1 cKO in PV neurons modulates neuronal activity in the cortex
Three forebrain structures, the prefrontal cortex, the amygdala and the hippocampus, form a dynamic neuronal network to regulate anxiety-related behaviors and modulate social interaction (54). To determine the effect of CTNNB1 KO in overall neuronal activity, we sought to determine c-Fos expression in the three brain regions of awake animals. Under a stimulated condition, in which the mice received a brief foot shot 1 h before sacrifice, we found that c-Fos positive cells was significantly reduced in the prefrontal cortex (Fig. 8A), but not in the hippocampus subregions and the amygdala of CTNNB1 cKO mice ( Fig. 8B and C). Particularly, majority of c-Fosþ cells were detected in the layer II (P ¼ 0.03) and V (P ¼ 0.006) of the prefrontal cortex. In either layer c-Fosþ cells were significantly decreased in cKO mice (Fig. 8A), suggesting an elevated level of inhibition. Although a similar damping trend was observed in the amygdala (P ¼ 0.07) (Fig. 8B), it didn't reach the statistical significance. This data is consistent with an increased inhibition in the cortex due to a higher density of PV interneurons (Fig. 1A), suggesting that PV circuits in the prefrontal cortex may contribute to CTNNB1 cKO mediated behavioral changes.

Discussion
Our results demonstrate that deleting CTNNB1 in PV interneurons significantly disrupts social and cognitive functions. This study provides the important evidence of CTNNB1's role in the inhibitory circuit in vivo. Our data links a deficit of CTNNB1 in PV interneurons directly to autistic-like behaviors. First, cKO of CTNNB1 in the PV interneurons causes anxiogenic effect in the OFT and EPM, but does not elicit depression-like behaviors, which is consistent with the increased anxiety in patients with ASD (55). Second, CTNNB1 cKO alters multiple cognitive functions. In particular, CTNNB1 loss-of-function impairs long-term memory in the NOR test and fear extinction. However, shortterm memory in the NOR and fear memory remain intact. Intriguingly, spatial memory is enhanced by CTNNB1 cKO in PV interneurons. Third, KO of CTNNB1 in the PV interneurons leads to deficit in social interaction and increased repetitive behaviors that mimic core symptoms of ASD patients. Moreover, neuronal activation was significantly reduced in the cortex of cKO mice. Thus, these results support that an optimal level of CTNNB1 in either inhibitory or excitatory neurons is critical to keep normal cognitive and emotional functions.
Different from the phenotypes observed in mice with CTNNB1 KO in excitatory neurons (25), which exhibit depression-like behavior, but not anxiety-related behaviors, we found that PV interneuron-specific cKO leads to increased anxiety without depression phenotype. PVþ interneurons are fastspiking neurons that inhibit excitatory activity. Our data suggest that CTNNB1 in the inhibitory circuit controls opposite behaviors observed in the excitatory circuit. Consistently, CTNNB1 in the nucleus accumbens confers pro-resilient and anxiolytic effects through a microRNA-dependent mechanism (56), supporting a critical role of CTNNB1 in regulating anxiety. Furthermore, CTNNB1 stability is controlled by GSK3b, which is implicated in mental illness. Lithium, a widely-used mood stabilizer for patients with bipolar disorder, inhibits GSK3b in vivo (57). GSK3b is a major mediator of anxiety and the lithium effect (58,59). Overexpression of CTNNB1 in the mouse brain phenocopies the anti-depressant effects of lithium in the amphetamine model (60), suggesting that lithium modulates behaviors through the GSK3b-CTNNB1 pathway. Our data, together with these studies, demonstrate that an optimal level of CTNNB1 in both excitatory neurons and inhibitory neurons is critical for normal emotional behaviors.
Here we showed that a novel role of CTNNB1 in PV interneurons to modulate social interaction and spatial learning. PV interneurons control the synchronization of the brain activities and are essential in learning behaviors. Imbalance of excitation and inhibition has been implicated in multiple psychiatric disorders, such as schizophrenia (61,62) and ASDs. Consistent with its critical role in memory, we found that PV interneuronspecific KO causes deficits in objective recognition and social memory. The short-term memory and contextual fear learning in PV interneuron-specific cKO mice were not affected, suggesting that memory acquisition was intact. Interestingly, the results that CTNNB1 PV-KO mice failed to recognize the second novel object in the NOR test and the second stranger mouse in the SIT, as well as to extinguish fear memory, indicate a deficit in the ability to acquire new information and to replace previous memories. This cognitive inflexibility is seen in autistic patients (63,64) and is consistent with phenotypes of mice expressing stabilized CTNNB1 during reversal learning tasks (24). Intriguingly, our mouse model showed enhanced spatial memory, which is consistent with other autistic mouse models with defect in Shank1 (49) and neuroligin-3 (45), but not with others (46,65). Increased spatial memory could be a CTNNB1 specific effect. However, patients with ASDs can display normal or, rarely, even enhanced cognitive abilities (66,67). Thus, we cannot exclude the possibility that this alteration by CTNNB1 KO could contribute to ASD pathophysiology as the genetics and symptoms of ASDs are quite heterogeneous.
Our study indicates that CTNNB1 cKO in PV interneurons is sufficient to modify the circuit development, resulting in more PV interneurons localizing in the cortex region even though PV is mainly expressed postnatally. CTNNB1 is the key mediator in the canonical Wnt signaling pathway, which involves a variety of cellular processes, including development, cell proliferation, cell survival, and motility. During early neurodevelopment, CTNNB1 fine-tunes the balance of proliferation and differentiation of neural stem cells. Both loss-and gain-of-function of CTNNB1 resulted in neurodevelopmental deficits (8,10). Additionally, the Wnt signaling pathway controls interneuron differentiation (68), thereby balancing the excitatory and inhibitory signals at a cellular level. Since PV is expressed postnatally (69), CTNNB1 does not likely work by regulating interneuron progenitor differentiation. Our data indicated that the localization of PVþ interneurons was not changed by CTNNB1 KO at P22 in the cortex. As at this age, proliferation and fate specification of interneuron progenitors have completed, our data suggest that mislocalization of PV cells occurs between P22 and P60, and is likely caused by a migration defect. Moreover, the number of PV þ cells in other brain regions was not changed. PV has not been shown as a direct downstream target of the Wnt pathway. This further supported that the change of PV distribution is not caused by abnormal proliferation, or altered fate determination, or modulation of PV expression. CTNNB1 might modulate migration via a-catenin-extracellular matrix interaction. However, the exact molecular mechanism on how CTNNB1 in PV neurons controls their distribution remains to be determined.
Our findings provide new insights into human CTNNB1 gene mutations in the inhibitory circuits as a risk factor for ASD/ID. Mutations in the Wnt pathway are enriched in the genetic risks in ASD (6). Consistently, mutations of CTNNB1 were identified in patients with ASD (6) and ID (9). Previous studies did not identify autistic-like behaviors, including anxiety, social interaction deficit and repetitive behaviors, on mouse models with CTNNB1 KO in excitatory neurons (22,24,25,60). Our study reveals that CTNNB1 KO in PV interneurons leads to cognitive and autistic-like phenotypes, suggesting that CTNNB1 defects in PV interneurons are responsible for the core symptoms of autisticlike behaviors. CTNNB1 interacts with a-catenin and N-cadherin to form cell adhesion complex localized at cell membrane and in synaptic junctions (21,70). It controls the synaptic strength and modulates neuronal plasticity in excitatory neurons (15,16,18,20). CTNNB1 may also regulate the electrophysiology of interneurons. Consistent with this notion, c-Fos staining was significantly reduced in the cortex, but not in the dentate gyrus and the amygdala, suggesting that an increased inhibition in the cortex, which may contribute to CTNNB1 KO-mediated behavioral changes. This data is consistent with increased inhibition in the cortex due to a higher density of PV interneurons. Future studies will be required to further delineate the underlying pathophysiological mechanisms. It would be interesting to further narrow down if CTNNB1 deletion from different brain regions or interneuron subtype will mediate particular phenotypes. Such studies will provide a better understanding of circuitry mechanism for ASD and might be potentially useful to design a new strategy to alleviate ASD symptoms.

Animals
The CTNNB1 flox/flox mice (B6.129-CTNNB1 tm2Kem /KnwJ) and the PV-Cre mice (B6; 129P2-Pvalb tm1(cre)Arbr /J) from the Jackson Laboratory (Bar Harbor, ME) were used to produce the heterozygous offspring. PV-Cre; CTNNB1 flox/flox mouse model were generated by backcrossing of F1 hybrids. All mice (two to four mice per cage) were housed at an ambient room temperature (20-22 C) with a 12-h light/12-h dark cycle (lights on at 6:00 am), and given ad libitum access to food and water. All animal

Immunohistochemistry
Mice were anesthetized with Avertin (200 mg per kg of body weight) and perfused with artificial cerebrospinal fluid and 4% performic acid (PFA). To investigate the neuronal activity using c-Fos immunohistochemistry, mice were stimulated by three times of 2-s, 0.55-mA foot shock within 250 s and put back to the original cage. One hour after the stimulation, mice were perfused. After post-fixation with 4% PFA for 24 h, brains were sliced by vibratome at the thickness of 50 mm. Each slide was pre-blocked with 5% donkey serum and 0.2% Triton X-100 in phosphate-buffered saline (PBS) for 1 h at room temperature. Primary antibodies were mixed in fresh blocking solution and incubate with brain slides for overnight at 4 C. After washing with PBS and 0.1% Triton X-100, brain slides were incubated with secondary antibodies conjugated with fluorescent groups. Brain slides were mounted to glass slides and photographed by confocal microscope. Image J was used for analysis of images. Antibodies used in this study are: rabbit anti-c-Fos antibody (Sigma), rabbit anti-PV antibody (Synaptic Systems), mouse anti-CTNNB1 antibody (BD Transduction Laboratories).

Open field test
The test was conducted as described in (46) with modifications. Briefly, each individual mouse was randomly placed at one corner of an opaque, open acrylic box (40Â40Â40 cm) in a brightly lit room. Video camera recorded horizontal movement of the mouse for 5 min. Total distance traveled, duration and entry frequency in the center (13.3 Â 13.3 cm), as well as the latency to the center region, were measured by EthoVision XT software. The open field apparatus was cleaned with 70% ethanol between each trial.

Elevated Plus Maze test
The test was performed as reported in (39). The maze is a fourarmed acrylic platform with a plus shape. The platform is elevated 50 cm above the ground, and two of the arms (5 Â 25 cm) were enclosed with high walls, and the other two arms were open. Each mouse was placed into the crossing region (5 Â 5 cm) of open and closed arms. The arms entry frequency, latency, duration and distance traveled were measured time spent in the closed and open arms were measured by EthoVision XT video tracking system. The plus maze apparatus was cleaned with 70% ethanol between each trial. Distribution of c-Fosþ cells did not showed a significant change at the hippocampus regions. *P < 0.05. **P < 0.01.

Novel Object Recognition test
In this test, mice were at first allowed free exploration for 5 min to acclimate with the testing environment in the open field arena. After the habituation session, mice interacted with two identical objects for 5 min. At 1 h after the habituation, the novel object 1 replaced one of the familiar objects. After 24 h, another novel object replaced the novel object 1. The amount of time mice spent with each object was measured by EthoVision XT to test the short-and long-term memory. Three groups of objects were with different shape, material and color, and randomly selected to serve as familiar objects or novel objects. The testing box was cleaned with 70% ethanol between each trial.

Social interaction test
The SIT was performed as reported with modifications (72). All the mice were single housed for 1 week before the tests. Mice were allowed to habituate with the three-chamber (each 20 Â 40 Â 20 (h) cm) box with two empty steel wire pencil cups (diameter 7 cm, height 10 cm) placed in center of the left or right chamber for 10 min. After habituation, one gender-matched wildtype mouse was placed in one cup. The wild-type mouse has never been exposed to the test mouse. The tested mouse was allowed to interact with either the empty cup or the cup with mouse for 10 min. At the other 10-min session, a stranger mouse was placed in the empty cup to test social memory. The frequency and time spent interacting with mouse or the empty cup were measured manually. Attempting to climb over or behind the cups was not recorded as an interaction. The testing box was cleaned with 70% ethanol between each trial.

Morris water maze
The MWM was done as reported in (73) with a period of 5 days, which included four training sessions and one test session. A transparent platform with a diameter of 10 cm was placed in the center of one quadrant of the pool (diameter 150 cm). The top of the platform was placed 2 cm below the water surface. Three other quadrants were labeled with different sign using color tapes for providing mice position cues. Nontoxic white watercolor paint was mixed into the water until the platform was no longer visible.
On training days, each mouse was placed into a randomly selected quadrant of the pool (a random number generator provided a number from one to four). If the mouse did not find the platform within 60 s, it was guided there by hand and allowed to stay there for 15 s. This was repeated three times daily for all the mice. On the testing day, the platform was removed and a circular platform zone was marked in the EthoVision software. The mice were individually placed into the pool and allowed to search for the platform. The times spent in the platform zone, as well as three larger circles concentric to the zone, were measured manually from the recorded videos. Quadrant duration and enter frequency were measured by EthoVision TX. All data are shown as mean 6 SEM and analyzed by Student's twotailed, unpaired t-test.

Grooming test
This test was used to score the repetitive behaviors (74). Each mouse was misted with a spray of distilled water towards to its face and placed into an empty cage without bedding. The animals were filmed for 25 min each. Accumulated grooming time was measured manually from the video. The first 5 min were excluded from the analysis because the variations in grooming duration during that time seemed largely dependent on the amount of water with which the mouse had been misted.

Marble burying test
This test was performed as reported in (75). Each mouse was placed into a new cage with 3 cm of bedding and five rows of four marbles on the surface of bedding. Leaving the mice in the testing cages for 10 min, we counted the number of marble buried and measured the latency to the first bury. All data are shown as mean 6 SEM and analyzed by Mann-Whitney U test.

Tail suspension test
Each mouse was secured to a horizontal rod about 60 cm above the floor using the end of its tail. The mouse was recorded for 5 min. Immobility time (when the mouse did not curl, move its paws, or swing across its vertical axis) (76) was measured manually from the video with a stopwatch.

Rotarod test
This test was done to exclude the possibility of motor deficits in Cre-positive mice that may result in misleading behavior test results (77). Each mouse was placed on the apparatus at four revolutions per minute (RPM). The speed accelerates from 4 RPM to a max of 40 RPM in 5 min, and the maximum speed is maintained for 5 min. We recorded the falling time of each mouse, and repeat the procedure three times per day for 3 days. On the eighth day, mice were tested with the same procedure. The rotarod apparatus was cleaned with 70% ethanol between each trial.

Nest building test
This test was performed as described in (78). Single-housed mice were transferred to new cages with 2 g nestlet in each cage. Assess the nests after 1, 3, and 24 h on a rating scale of 1-5. Scores were analyzed using Kruskal-Wallis ANOVA.

Fear conditioning and extinction test
In the training session, each mouse was habituated in the fear conditioning apparatus for 5 min. After the habituation, the mouse in the testing chamber will receive a 2-s, 0.55-mA foot shock every 80 s (79). The mouse was removed immediately after the third shock. During the contextual testing, the freezing behavior of each mouse was recorded in the testing chamber every 24 h until reaching the significant difference between two groups.

Statistical Analysis
Data were analyzed using Excel and SPSS software and are expressed as means 6 SEM. Significances between the experimental group and control group were analyzed by Student's t-test and ANOVA.

Supplementary Material
Supplementary Material is available at HMG online.