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An improved technique for transauricular hypophysectomy and auto transplant of isolated pieces of pars intermedia tissue in the evacuated pituitary capsule of the rat.

An improved technique for transauricular hypophysectomy in rats, weighing 120-300 g, is described. This method can be used for transplantation studies, which is demonstrated by transplanting pars intermedia tissue into the evacuated pituitary capsule. Our technique has been carried out, successfully, in various laboratories. The success, however, depends upon the skill and care of the investigator.