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The Fya, Fy6 and Fy3 epitopes of the Duffy blood group system recognized by new monoclonal antibodies: identification of a linear Fy3 epitope

Four new anti‐Duffy murine monoclonal antibodies (MAbs): two anti‐Fy6 (MIMA‐107 and MIMA‐108), one anti‐Fya (MIMA‐19) and one anti‐Fy3 (MIMA‐29) were characterized. Identification of epitopes by means of synthetic peptides (Pepscan) showed that the anti‐Fy6 reacted most strongly with peptides containing the sequence 19QLDFEDV25 of the Duffy glycoprotein, and less strongly with peptides containing LDFEDV (MIMA‐107) or LDF only (MIMA‐108). The anti‐Fya recognized epitope 38DGDYGA43 containing the Gly42 residue, which defines the Fya blood group antigen. MIMA‐29 is the first anti‐Fy3 reactive with a linear epitope 281ALDLL285 located in the fourth extracellular domain (ECD4, loop 3) of the Duffy glycoprotein. The four new antibodies extend the list of six anti‐Fy MAbs formerly characterized by Pepscan analysis that allow some general conclusions. Fine specificities of various anti‐Fya, or anti‐Fy6 are not identical, but all of them recognize linear epitopes located around, respectively, Gly42 or between two potential N‐glycosylation sites at Asn16 and Asn27. Anti‐Fy3 recognize either a linear epitope located in ECD4, or a conformational epitope that includes amino acid residues of ECD4 and of other ECDs.