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Biocatalysis of chlorophyllase from the alga Phaeodactylum tricornutum in a water/miscible-organic-solvent system

The hydrolytic activity of a partially purified chlorophyllase obtained from the alga Phaeodactylum tricornutum was determined in different mixtures of acetone and Tris/HCl buffer solution (20 mM, pH 8.0) used as water/miscible-organic-solvent system. The most appropriate concentrations of acetone for chlorophyll-ase activity were 17.5 and 30% respectively at 25 o C and 32.5 o C incubation temperatures. Using the optimized water/acetone ratio, the optimal pH for chlorophyllase activity was found to be 8.0, whereas the time of incubation and the amount of required enzyme were 2 h and 0.30 μg of protein respectively