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Cyclosporine measurement in transplantation.

Use of cyclosporine is a major breakthrough in immunosuppressive therapy for organ transplantation. Critical to its success, however, is the availability of regular and frequent measurements of the blood levels of the drug. Radioimmunoassay and/or high-pressure liquid chromatography may be employed for this analysis. Radioimmunoassay measures the parent molecule together with its metabolites, whereas high-pressure liquid chromatography measures only the parent drug, which indicates that clinical and laboratory experience, as well as an understanding of methodology, are necessary for the interpretation of cyclosporine levels.