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OPTIMIZATION OF RECOMBINANT LACCASE PRODUCTION BY YARROWIA LIPOLYTICA IN A MEDIUM CONTAINING GLUCOSE AS CARBON SOURCE WITH TAGUCHI METHOD

Introduction: Laccases (EC 1.10.3.2; benzenediol: oxygen oxidoreductase) are coppercontaining oxidases that use molecular oxygen to oxidize various aromatic and non-aromatic compounds. Laccase is applied in delignification of lignocellulosic compounds for production of bioethanol, bioremediation of industrial wastewaters especially textile, food industries, and making biosensors. Materials and methods: The Taguchi experimental design method was used for optimization of laccase production in recombinant strain Yarrowia lipolytica YL4. A L-16 Taguchi orthogonal array was used to optimize the carbon and nitrogen sources along with vitamin in four levels. Results: The results showed that glucose, ammonium chloride, yeast extract and thiamine have significant effects on the production of laccase, respectively. The laccase activity reached to 1.52 U/mL after optimization of medium which is 7.6-fold higher than un-optimized medium. Discussion and conclusion: According to the analysis of results, the Taguchi experimental design method is a successful approach to increase laccase and recombinant proteins production in Y. lipolytica.