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Q Fever as a Cause of Community-Acquired Pneumonia in French Guiana

ABSTRACT. In French Guiana, community-acquired pneumonia (CAP) represents over 90% of Coxiella burnetii acute infections. Between 2004 and 2007, we reported that C. burnetii was responsible for 24.4% of the 131 CAP hospitalized in Cayenne. The main objective of the present study was to determine whether the prevalence of Q fever pneumonia remained at such high levels. The secondary objectives were to identify new clinical characteristics and risk factors for C. burnetii pneumonia. A retrospective case-control study was conducted on patients admitted in Cayenne Hospital, between 2009 and 2012. All patients with CAP were included. The diagnosis of acute Q fever relied on titers of phase II IgG ≥ 200 and/or IgM ≥ 50 or seroconversion between two serum samples. Patients with Q fever were compared with patients with non-C. burnetii CAP in bivariate and multivariate analyses. During the 5-year study, 275 patients with CAP were included. The etiology of CAP was identified in 54% of the patients. C. burnetii represented 38.5% (106/275; 95% CI: 31.2–45.9%). In multivariate analysis, living in Cayenne area, being aged 30–60 years, C-reactive protein (CRP) > 185 mg/L, and leukocyte count < 10 G/L were independently associated with Q fever. The prevalence of Q fever among CAP increased to 38.5%. This is the highest prevalence ever reported in the world. This high prevalence justifies the systematic use of doxycycline in addition to antipneumococcal antibiotic regimens.


INTRODUCTION
Q fever is a zoonosis caused by Coxiella burnetii, an intracellular bacterium, which is present worldwide. 1 The primary infection is called acute Q fever and can manifest itself as isolated fever, hepatitis, or pneumonia. 1 It is a public health problem in the area of Cayenne, French Guiana, a French overseas territory located in the Amazonian area of the northeastern part of South America. Although the incidence of Q fever in French Guiana is the highest in the world, by contrast, it is exceptionally described elsewhere in Latin America. [2][3][4] A unique clone, the strain MST17, is responsible for most of the cases in French Guiana and shows a greater virulence than classical strains in animal models. [5][6][7] While human cases are generally linked to the contact with goats or sheep worldwide, no direct evidence of carriage in cattle, goats, or sheep has been found in French Guiana. However, so far, it has been found in a few animals species, such as the three-toed sloth (Bradypus tridactylus), around the city of Cayenne, and more recently in capybara (Hydrochoerus hydrochaeris) in the context of a rural outbreak. 7,8 Regarding clinical features, while community-acquired pneumonia (CAP) generally represents less than 50% of cases of primary C. burnetii infection in most series, it represents more than 90% of cases in French Guiana. [9][10][11] Moreover, C. burnetii represents the main pathogen responsible for hospitalized CAP in Cayenne. We reported, between 2004 and 2007, that C. burnetii was responsible for over 24% of 131 CAP admitted in Cayenne General Hospital, the reference hospital in French Guiana. 11 This was the highest prevalence of Q fever pneumonia ever reported in the literature, where it only ranges from 0% to 3% of CAP, depending on the region and the type of CAP (community, in hospital, intensive care unit).
The local treatment recommendations in Cayenne Hospital integrate this epidemiology and cover C. burnetii in the empirical treatment of CAP. The most prescribed empirical antibiotic regimen for CAP, supported by the general practitioners and the emergency department, is a combination of amoxicillin and doxycycline. Currently, the diagnosis of acute Q fever is obtained by serology, with seroconversion during the primary infection occurring between day 7 and day 21 after the onset of the symptoms, thus, in the absence of a local point of care quantitative polymerase chain reaction (qPCR) technique, other tools are useful to help choosing the antibiotic regimen. Our first publication led to a predictive score to promptly identify Q fever among hospitalized CAP. 11 Male sex, middle age (30-60 years), headache, leukocyte count , 10 G/L, and C-reactive protein (CRP) level . 185 mg/L were thus independently associated with Q fever and were used in the predictive score. 11 Unfortunately, exposure risk factors were not analyzed in this study. However, some exposure risks factors for Q fever in French Guiana have been described, such as living near the forest, frequently seeing bats, marsupials, or wild mammals near the house, as well as owning an air-conditioned vehicle, performing leveling work near the house, gardening, working in the building trade or public work, or house cleaning. 10,12 Furthermore, since the previous study, C. burnetii DNA was detected in a dead three-toed sloth found next to a hill where an outbreak of Q fever had occurred, and carrying a three-toed sloth in arms was an independent risk factor for acute Q fever. 7,12 The very high prevalence observed in this previous study may reflect two possible epidemiological situations: an endemic situation with persistent high incidence or an outbreak at the time of the study. To sort between these two scenarios, we completed data collection for longer stretches of time to see if the trends were the same. Thus, the main objective was to evaluate acute Q fever prevalence among CAP between 2009 and 2012. The secondary objectives were to identify clinical characteristics and risk factors for acute Q fever.

METHODS
Setting. In French Guiana, the Amazonian rain forest covers over 98% of the 84,000 km 2 territory. 13 The remaining part in the north is a coastal plain where 90% of the 215,000 inhabitants live, with Cayenne and surroundings (including R emire-Montjoly and Matoury) concentrating almost 50% of the population in 2016 (URL: www.insee.fr). Its location a few degrees from the intertropical convergence zone brings a humid equatorial climate, with the alternance between wet and dry season rhythming natural cycles and human activities.
Patients. We retrospectively analyzed patients with CAP admitted in the Department of Infectious and Tropical Diseases of Cayenne Hospital, French Guiana, between January 1, 2009, and December 31, 2012. Cayenne Hospital is a general hospital and the only hospital of the territory with an infectious diseases department (20 beds) and advanced microbiological diagnostic facilities for the etiologies of CAP; it is in close vicinity to the Institut Pasteur in French Guiana, which performs C. burnetii serology.
Inclusion criteria. CAP criteria. All patients who had an acute onset (# 7 days) of respiratory signs, which were defined as cough, chest pain, expectoration, and dyspnea, associated with at least one of the following signs: body temperature . 38 C, auscultation suggestive of pneumonia (crackles, wheezing, diminution of vesicular murmur, and vocal vibrations), and radiographic evidence of pneumonia (alveolar syndrome, interstitial syndrome, bronchial involvement, and pleural effusion) were considered to have CAP.
Microbiological definition of an acute Q fever case. Screening serologic tests were performed at the Institut Pasteur de la Guyane, and diagnosis was confirmed by the French National Reference Center for Q fever, Marseille, France, with an in-house indirect immunofluorescence assay, using C. burnetii Nine Mile strain phase I and phase II antigen, as previously described. 14 Serodiagnosis of Q fever was performed on all patients with CAP using IgG and IgM antibodies against phase II and phase I antigens of C. burnetii in an indirect immunofluorescence assay. Criteria for a positive C. burnetii serology titers of phase II IgG $ 200 and/or IgM $ 50. Criteria for the diagnosis of acute Q fever were the following: seroconversion, 15 identified between two serum samples (one from the acute phase and one from the convalescent phase) OR one positive serology (phases I and II) associated with no other identified etiology and diagnosis of Q fever by the clinician in charge of the patients, treated by doxycycline.
CAP microbiological screening. Patient with CAP of unknown or other etiology were defined as controls. While all patients were routinely serologically tested for C. burnetii, due to the known importance of this infectious pathogen in French Guiana, microbiological diagnosis of other respiratory infectious agents was performed at the discretion of the attending physician. Diagnostic tests for other pneumonia pathogens were performed. Serologic tests were used to detect Mycoplasma pneumoniae, Chlamydia pneumoniae, Chlamydia psittaci, Legionella pneumophila, Toxoplasma gondii (its Amazonian strains causing severe pneumonia), and Bordetella pertussis. The identification of L. pneumophila and Streptococcus pneumoniae were based on the detection of specific antigens in urine samples by an immunochromatographic assay and blood cultures. Serologic tests and qPCR were performed to detect arbovirosis, measles, and leptospirosis. If pulmonary tuberculosis was suspected, specific direct examination and cultures of sputum smears were performed.
Ethics statement. Patient's medical records were retrospectively reviewed. All data collected were anonymized using standardized forms according to the procedures of the Commission Nationale de l'Informatique et des Libert es (the French information protection commission). The study was approved by the local ethics committee as this research was consistent with the French MR004 reference methodology and fell within the framework of so-called "internal" research, that is, the data were collected as part of the individual follow-up of patients, by the staff ensuring this follow-up and for their exclusive use. In accordance with the European regulation on data protection, the study was registered in the hospital's registry of processing activities by the person in charge of data protection, and collective information was made available to patients by means of a poster in the department, allowing them to express their refusal to participate, if necessary.
Data collection and analysis. The acute Q fever and CAP cases were identified retrospectively using the French PMSI (Programme M edicalis e des Syst emes d'Information) through the extraction of all patients receiving diagnostic codes of the CIM-10 for pneumonia: J12, J13, J15, J16, J17, J18, and the A78 code for Q fever.
A standardized form was completed retrospectively for data collection, including socio-epidemiologic data, clinical symptoms of the first clinical examination, and radiographic and biological results. Risk factors were also included in the data collection. The variable was considered positive if present in the file and negative if absent. All data were recorded retrospectively with Microsoft Excel. The continuous variables of interest were categorized following the laboratory cut-off values or values in the medical literature. They generally were dichotomized because of the sample size.
Determination of risk factors. A case-control study was performed to identify factors associated with Q fever among CAP, comparing patients with Q fever CAP (cases), to patients with CAP of other etiology or unknown etiology (controls). Statistical analysis of data was performed using the SPSS Software (IBM), version 22.0. Categorical variables were described using frequencies and percentages and compared using Fisher's exact test. Continuous variables were described using means 6 SDs or medians with interquartile ranges; they were compared using the Mann-Whitney test. Logistic regression analysis was performed. Potential variables associated with Q-fever CAP occurrence were selected using bivariate logistic regression. The variables retained after the bivariate analysis (P , 0.20) were introduced in a multivariate logistic regression model with a backward step-by-step selection procedure. The significance level p was fixed at 0.05. The estimated adjusted odd ratios (OR) were presented with their 95% CI (95% CI).
Review of the literature about acute Q fever prevalence. After identification of a high prevalence of Q fever CAP in our study, we performed a search in Medline with the following keywords: [ Table 2). Twenty-nine patients were diagnosed based on a seroconversion, 61 patients were diagnosed based on elevated phase II IgG and IgM titers (both positive on two serum samples) without seroconversion, 13 patients were diagnosed based on a one-off serology with both positive phase II IgG and IgM, and 3 patients were diagnosed based on a one-off positive phase II IgM. The prevalence of Q fever among acute CAP fluctuated from year to year with no clear trend, ranging from 29.2% (2010) to 68.8% (2009) ( Table 3). The second most frequent bacterium was Mycoplasma pneumoniae in 21 patients (7.6%), followed by Streptococcus pneumoniae (1.8%) and the Amazonian strain of Toxoplasma gondii, so-called Amazonian toxoplasmosis, for 4 patients (1.5%). Most patients presenting with CAP in Cayenne Hospital had fever (90%) and cough (66%) and radiological abnormalities were present in 83% of cases (Table  1). Digestive manifestations (abdominal pain, diarrhea, and vomiting) were present in 53% of cases and eye, nose, and throat symptoms or signs were reported in 47% of patients.
Variables associated with Q fever versus other etiology among CAP. In bivariate analysis, sociodemographic characteristics of patients associated with C. burnetii CAP versus other etiologies were age between 30 and 60 (P , 0.001), being born in mainland France (P 5 0.03), living in Cayenne and its suburbs (P , 0.001), reporting tobacco and frequent alcohol consumption (P , 0.001 and P 5 0.01, respectively), and the notion of leveling work near the house and gardening activities (P , 0.001) ( Table 1).  Regarding clinical features, the presence of temperature . 38.5 C, headaches, chills, muscle pain, and abnormal auscultation were associated with acute Q fever CAP in bivariate analysis (Tables 4 and 5). In addition, acute Q fever CAP were significantly associated with the absence of cough and eyes nose throat (ENT) symptoms in bivariate analysis. The analysis of biological data found that white blood cell count (WBC) inferior to 10 G/L, polymorph nuclear (PMN) count inferior to 7.7 G/L, CRP . 185 mg/L, and serum glutamo pyruvate transaminase (TGP) and/or serum glutamo oxaloacetic transaminase (TGO) greater than 1.5 times the normal range were significantly associated with Q fever CAP in bivariate analysis (Tables 1 and 4).
In the multivariate analysis, living in Cayenne area, being aged between 30 and 60 years old, having a CRP levels . 187 mg/L, and having WBC counts ,10 G/L were independently associated with Q fever (Table 4).

DISCUSSION
The highest prevalence in the world ever described among CAP. While our previous study reported that almost 25% of the CAP hospitalized in the hospital of Cayenne were due to C. burnetii infection, the present study, spanning the 5 years after the initial study, now found that 38.5% of CAP were Q fever cases; hence, a prolonged period with a very high proportion of Q fever cases, which is more in favor of high endemicity than a punctual outbreak. 11 This is the highest proportion of Q fever among pneumonias ever reported in the world, even in epidemics ( Table 6). Most of the international series show a prevalence of Q fever among pneumonia from 0% to 3%, the higher proportions being linked to epidemics. [16][17][18][19] Furthermore, French Guiana is located in Latin America where Q fever is hardly reported, especially in pneumonias. 4,[20][21][22][23][24] The increase of the prevalence between the 2004-2007 and 2009-2012 period maybe due to a greater awareness of physicians after the first study, and a greater use of the serodiagnostic test. Indeed, in our previous study, the second sample at day 14-21 after the beginning of the symptoms was not systematically performed, while in the second study period, the seroconversion was more systematically screened with an early and a late sample. Thus, the result of 38.5% of C. burnetii among   Recent studies have demonstrated that the unique clone MST17 found in French Guiana is more virulent in silico, in vitro, and in vivo than the reference strains Nine Mile and the Z3055 strain, isolated from an ewe in Germany and phylogenetically very close to the strain that caused an important outbreak in the Netherlands, 5,6,25 potentially explaining this high prevalence, at least in part. Also, the spread of the reservoir still remains unclear. The first reservoir identified was the three-toed sloth (Bradypus tridactylus), in the Cayenne area. However, the recent detection of C. burnetii DNA in capybara (Hydrochoerus hydrochaeris) 7,8 in the context of a rural outbreak far from the island of Cayenne, suggests that several animals living in the Amazonian rainforest could be involved in the dissemination of C. burnetii infection. The first reported case of acute Q fever in Cayenne in 1998 was observed in a slaughterhouse worker, so that one could hypothesize that the bacterium may have been transmitted from its classical reservoirs to new hosts in a territory of high wild biodiversity. Moreover, these wild animals are also present in the rest of French Guiana and in all Amazonian countries. Further studies are required to understand this very high prevalence, especially in the Latin-American context, and to clarify the patterns of the wild/domestic reservoir and population features.
Very specific features associated with C. burnetii infection. In the present study, we found the same features that had been described before, which are confirmed to be quite specific. Coxiella burnetii pneumonia is more frequently found in men, aged between 30 and 60 years old. This is often observed in zoonoses linked to professional or leisure outdoor activities and is quite close to risk groups found elsewhere. 10,26 However, the two new very specific characteristics in French Guiana are that people born in mainland France, which represent less than 10% of the global population of the territory, were over-represented here, and had a 2-fold increase of acute Q fever than patients of other origins; however, this was no longer significant in multivariate analysis. Finally, living in Cayenne and its surroundings remained associated with the risk to get acute Q fever compared with people living elsewhere. A hot spot of Q fever was reported in the town of R emire-Montjoly, located at the immediate East of Cayenne, without clear explanations. The city of R emire-Montjoly is characterized by housing estates separated by hills of the Amazonian rainforest, like Bourda and Rorota hills. Previous work based on geolocation of cases had shown that these two areas were marked by the very high incidence of Q fever. 10 These forest hills are home to three-toed sloths, one of the putative reservoirs of the disease. This difference between "Caucasian people," sometimes including people of various color of skin but born in mainland France, compared with people born in the region may be explained by a higher immunity in the native population. Further seroprevalence studies in French Guiana could be interesting to further investigate this phenomenon. Also, one could hypothesize that people from mainland France would be more likely to consult in the hospital than those from other cultural groups, but this is not confirmed by our results showing that natives of French Guiana represented 40% of hospitalized CAP versus 23% of people born in mainland France (Table 3).

EPELBOIN AND OTHERS
Finally, we found environmental exposure risk factors, such as gardening or work involving soil near homes. These activities had already been described earlier, and may be associated with an aerosolization of the dust, which may contain particles of the bacterium. 8,10,12 Unfortunately, this study was performed before the discovery of the possible carriage of C. burnetii by wild fauna, such as three-tooth sloth and capybara, so the survey could not question the contact of the patients with these animals. 7,8 Practical consequences of these results. Although the first publication suggested that doxycycline should be used only in patients with a high probability of Q fever, the results of the present study are in favor of systematic use of an anti-C. burnetii antibiotic regimen when facing a clinical and radiological picture of CAP due to its exceptionally high prevalence. Macrolides, which are the second-choice antibiotic regimen in addition to the antipneumococcal treatment, should not be used as the Guianese strain MST 17 seems to be resistant to this antibiotic family. 27,28 Limitations of the study. This study is retrospective, and consequently suffers from several biases and unknown confounders. First, diagnostic criteria for Q fever CAP were heterogeneous, since seroconversion was not systematically identified due to the absence of follow-up for some patients. Second, etiologic investigations were left to the appreciation of the clinicians in charge of the patients, so that not all patients were tested for all the pathogens found in the study. However, we think that the high prevalence of Q fever in these real-life conditions is an important signal. Further prospective cohort studies with more stringent criteria are needed to confirm these data and to increase our knowledge about the long-term consequences of acute Q fever in French Guiana.
In conclusion, we observed a persistently high proportion of Q fever among adults with CAP in Cayenne Hospital. The relatively long time span seems more in favor of high endemicity than the punctual outbreak. This stable fact justifies the adaptation of treatment protocols for CAP, which should systematically cover for Q fever.