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Insight into the maintenance of odontogenic potential in mouse dental mesenchymal cells based on transcriptomic analysis

Background. Mouse dental mesenchymal cells (mDMCs) from tooth germs of cap or later stages are frequently used in the context of developmental biology or whole-tooth regeneration due to their odontogenic potential. In vitro-expanded mDMCs serve as an alternative cell source considering the difficulty in obtaining primary mDMCs; however, cultured mDMCs fail to support tooth development as a result of functional failures of specific genes or pathways. The goal of this study was to identify the genes that maintain the odontogenic potential of mDMCs in culture. Methods. We examined the odontogenic potential of freshly isolated versus cultured mDMCs from the lower first molars of embryonic day 14.5 mice. The transcriptome of mDMCs was detected using RNA sequencing and the data were validated by qRT-PCR. Differential expression analysis and pathway analysis were conducted to identify the genes that contribute to the loss of odontogenic potential. Results. Cultured mDMCs failed to develop into well-structured tooth when they were recombined with dental epithelium. Compared with freshly isolated mDMCs, we found that 1,004 genes were upregulated and 948 were downregulated in cultured mDMCs. The differentially expressed genes were clustered in the biological processes and signaling pathways associated with tooth development. Following in vitro culture, genes encoding a wide array of components of MAPK, TGF-β/BMP, and Wnt pathways were significantly downregulated. Moreover, the activities of Bdnf, Vegfα, Bmp2, and Bmp7 were significantly inhibited in cultured mDMCs. Supplementation of VEGFα, BMP2, and BMP7 restored the expression of a subset of downregulated genes and induced mDMCs to form dentin-like structures in vivo. Conclusions. Vegfα, Bmp2, and Bmp7 play a role in the maintenance of odontogenic potential in mDMCs.


INTRODUCTION
In the field of tooth regeneration, the main concept is to mimic the natural tooth development process using stem cells. Cells or tissues with odontogenic potential are required to regenerate a whole tooth (Kollar & Baird, 1970b;Mina & Kollar, 1987). Since the odontogenic potential to instruct tooth organogenesis shifts to the dental mesenchyme at bud stage of odontogenesis (Kollar & Baird, 1970a;Mina & Kollar, 1987), and dental mesenchyme determines the tooth type and size (Kollar & Baird, 1969), it will be preferable to use a mesenchymal cell population with odontogenic potential to provide the inductive signal. Nevertheless, the adult dental stem/progenitor cells appear to lack the potency to regenerate an anatomically correct complete tooth organ (Hu et al., 2014;Mao & Prockop, 2012), and the odontogenic potential of progenitor cells derived from the embryonic stem cells or induced pluripotent stem cells was unknown (Otsu et al., 2012;Ozeki et al., 2013;Seki et al., 2015). Mouse dental mesenchymal cells (mDMCs) isolated from dental mesenchyme of cap or later stages possess the odontogenic potential and they have frequently been used in the context of developmental biology or whole-tooth regeneration (Honda et al., 2007;Ikeda et al., 2009;Nakao et al., 2007). mDMCs are able to induce tooth formation in a dental or nondental epithelial cell population (Kollar & Baird, 1969;Kollar & Baird, 1970b;Wang et al., 2010;Angelova Volponi, Kawasaki & Sharpe, 2013;Cai et al., 2013). As the preparation of mDMCs is time-consuming and embryos at the right stage are not easily available, in vitro-expanded mDMCs could be an available cell source. However, mDMCs fail to support tooth development when they are grown as a monolayer (Keller et al., 2011), and the genes that contribute to the loss of odontogenic potential in mDMCs were unknown.
RNA sequencing (RNA-seq) technology provides sensitive detection of global gene expression profiles in cells and tissues, and bioinformatics approaches also examine changes in the context of the overall pathway rather than individual genes (Wang, Gerstein & Snyder, 2009). To identify the genes that maintain the odontogenic potential in mDMCs, we evaluated the odontogenic potential of freshly isolated versus cultured mDMCs from the lower first molars in embryonic day 14.5 (E14.5) mice, and compared the transcriptome of freshly isolated mDMCs with that of cultured mDMCs. We found a loss of odontogenic potential accompanied by a significant disturbed transcriptome in cultured mDMCs and identified potential genes that contribute to the loss of odontogenic potential, providing new insight into the maintenance of odontogenic potential of mDMCs.

Cell culture
All procedures involving animals described in the present study were reviewed and approved by the Animal Care and Use Committee of Guangzhou Institutes of Health and the Peking

Quantitative reverse transcription PCR (qRT-PCR)
Total RNA was extracted with TRIzol (Invitrogen, Carlsbad, CA, USA) and reverse transcribed using an RT-PCR kit (TaKaRa Bio, Otsu, Japan) (Zheng et al., 2014). Quantitative PCR was performed in a Thermal CyclerDice7 TM Real Time System with SYBR Green Premix EXTaq TM (TaKaRa Bio). The primers used are listed in Table 1. The relative expression of genes was calculated using the 2 − Ct method.

Tissue recombination and subrenal culture
The mDMCs were harvested at indicated time points using 0.25% trypsin (Sigma) and spun down to make cell pellets. The cell pellets were cultured at 37 • C for 2-3 h and then recombined with freshly isolated E14.5 dental epithelium. The recombinants were further cultured in vitro for 24 h prior to subrenal culture in adult ICR male mice. The host mice were sacrificed 3 weeks later to harvest the grafted tissue. Grafts were then fixed and subjected to H&E staining for histological analysis.

RNA isolation and sequencing
To determine the transcriptional regulation following in vitro culture, mDMCs from the developing molars in E14.5 mouse embryos were isolated and designated as P0. The cells were then subcultured in standard medium and passaged once they reached 90% confluence, with the first-passage culture designated as P1 and the second-passage culture as P2. P0, P1 and P2 cells were harvested using 0.25% trypsin (Sigma-Aldrich

Bioinformatics analysis
Sequenced reads were mapped to the mouse transcriptome (mm10, Ensembl v73) and then aligned using bowtie (v1.0.1) and RSEM (v1.2.12), as described previously (Hutchins, Takahashi & Miranda-Saavedra, 2015;Li & Dewey, 2011). EDASeq (v1.11.0) was used for GC normalization of samples, and differential expression was called using DESeq2 (v1.12.0). The fold change cut-off was set at twofold and p-value < 0.05 were considered to be statistically significant. The Database for Annotation, Visualization and Integrated Discovery (DAVID) was used to determine overrepresented GO categories and KEGG pathways using the entire mouse transcriptome as the background gene set. Protein-protein interaction network of DEGs was constructed using the STRING database (http://string-db.org/). Ingenuity pathway analysis (Ingenuity Systems, Redwood City, CA, USA) was used to analyze the upstream regulators of the differential expression between the samples (Razali et al., 2015).

Statistical analysis
Statistical analyses were performed using SPSS PASW version 18. Experiments with cell cultures were performed at least in triplicate and data are expressed as mean ± standard deviation (SD). The differences among groups were analyzed using one-way ANOVA. A two-tailed p-value < 0.05 was considered to be statistically significant.

RESULTS
mDMCs were isolated from the developing molars in E14.5 mouse embryos and were designated as P0. One part of the P0 cells was recombined with E14.5 dental epithelium and cultured in kidney; another part was subjected to RNA-seq. The remaining cells were subcultured in standard medium, with the first-passage culture designated as P1 and the second-passage as P2. P1 and P2 cells were also split into two portions to ensure an identical cell state for the recombination assay and the RNA-seq (Fig. 1).
Odontogenic potential of cultured mouse dental mesenchymal cells mDMCs are typically cultured in Eagle's minimum essential medium plus fetal bovine serum and antibiotics (Jiang et al., 2014;Keller et al., 2011;Zhao et al., 2014). mDMCs showed an atypical round-or spindle-shaped fibroblast-like morphology with a higher nuclear-to-cytoplasmic ratio, indicating their primitive character. Although the cells retained fibroblastic features up to the second passage, they exhibited a senescent phenotype with increased cytoplasm and augmented volume ( Fig. 2A).
The odontogenic potential of P0, P1, and P2 cells were examined to identify the impact of the culturing. Although freshly isolated mDMCs developed into well-structured teeth Tooth germs from embryonic day 14.5 mice were obtained and digested with dispase to separate the dental mesenchyme from dental epithelium. Freshly isolated dental mesenchymal cells were designated as P0 and cultured in vitro. RNA samples from the P0, the first (P1), and second (P2) passages were collected before they were submitted for RNA-seq using an Illumina Hiseq TM 2000.
when recombined with embryonic dental epithelium, cultured mDMCs failed to support tooth development (Fig. 2B). Moreover, the expression of genes that are essential for tooth development were analyzed using qRT-PCR. The expression of Msx1, Pax9, and Lhx6 was significantly reduced in P1 and P2 cells compared with P0 cells (Fig. 2C). The expression of Fgf3 and Bmp2 in cultured mDMCs was reduced compared with the P0 cells, but the expression of Bmp4 was not significantly different between the P0 cells and cultured mDMCs (Fig. 2D).

Overview of the mouse dental mesenchymal cells' transcriptome
To obtain a global view of genes regulating the loss of odontogenic potential, total mRNA of P0, P1, and P2 cells was extracted and sequenced. After data correction, 11,340 transcripts could be matched exactly to known mouse Ensemble transcripts. A total of 9,815 genes were shared among P0, P1 and P2 cells, whereas 563 genes were expressed exclusively in P0 cells (Fig. 3A). P0 cells that were not exposed to in vitro culture conditions showed a striking separation from P1 and P2 cells ( Fig. 3B; Fig. S1). The transcriptional disparity between freshly isolated and cultured mDMCs is consistent with their phenotypic differences. Differential expression analysis revealed that in vitro expansion of mDMCs promoted the selective overexpression of 859 genes, whereas 763 genes were downregulated in P1 cells (Fig. 3C). Comparison of the transcriptomes of P0 and P2 cells revealed that 1,004 genes were upregulated and 948 were downregulated (Fig. 3C). In contrast, 13 genes were upregulated and two genes were downregulated in P1 compared with P2 cells (Fig. 3C). These results suggested that the transcriptome of mDMCs was significantly influenced by in vitro culture conditions. In addition, the expression levels of Msx1, Lhx6, Pax9, Bmp4, Fgf10, Bmp2, and Fgf3 were comparable when analyzed with RNA-seq and qRT-PCR (Fig. 3D).

Gene ontology analysis of differentially expressed genes
Gene ontology (GO) analysis provides an intuitive and effective approach to understand the function of genes in three domains: biological processes, cellular components, and molecular functions. To understand the function of differentially expressed genes, GO analysis was conducted (Fig. S2) and a network diagram was created to illustrate the communication of differentially expressed genes in the enriched clusters of biological processes (Fig. 4). The network is composed of: (a) genes around the node Dnmt3b, namely Dnmt1, Ezh2, Uhrf1, and Rbl1, which participate in chromatin organization and transcriptional regulation; (b) genes around the node Runx2, namely Bmp2, Dlx1, Lhx6, and Sp7, involved in cell differentiation and skeletal system development; (c) genes around the node Fn1, namely Robo4, Tie1, Pecam1 and Col5a1, involved in cell motion and cell adhesion; and (d) genes that are not linked to any specific node, whose function is related to apoptosis (Tradd, Tnfrsf11b) and epithelial morphogenesis (Grem1). These various genes

Pathway analysis of the differentially expressed genes
Multiple pathways including fibroblast growth factor (FGF), bone morphogenetic protein (BMP), hedgehog (SHH), and Wnt signaling play critical roles in odontogenesis (Liu et al., 2013;Thesleff, Vaahtokari & Partanen, 1995); thus, the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was used to determine the pathways involving the differentially expressed genes (Fig. 5A)  numerous members of these signaling pathways were reduced in cultured mDMCs (Fig. 5B), and it was difficult to identify the critical regulators that are responsible for the loss of odontogenic potential in cultured mDMCs.
An upstream analysis predicts which upstream regulators were most likely to be involved based on the current levels of gene expression detected by RNA-seq analysis. Numerous upstream regulators were related to the loss of odontogenic potential in cultured mDMCs. Specifically, the activities of Bdnf, Vegf α, Bmp2, and Bmp7 were predicted to be significantly inhibited, while Igf2, Fgf10, and Tgfb1 were more active in cultured mDMCs (Fig. 5C). Although Bdnf was slightly increased in cultured mDMCs, its receptor Ntrk2 was significantly decreased (Fig. 5B). The inhibition of Bdnf led to the downregulation of genes that promote odontogenesis, such as Egr1, Egr2, and Fos (File S1). Downregulation of Bmp2 and Bmp7 probably contributed to the loss of odontogenic potential via the inhibition of Runx2, Sp7, and Msx2 (File S1).

VEGFα, BMP2, and BMP7 restore the expression of downregulated genes
Given the pivotal roles of VEGFα, BMP2, and BMP7 in tooth development, we selected these proteins to increase the expression of odontogenic genes. Indeed, tooth mesenchymespecific genes were upregulated including Msx1 and Pax9 in mDMCs treated with Bmp2 and Bmp7 (Figs. 6B and 6C). VEGFα slightly increased the expression of Msx1 and Pax9, but not that of Lhx6 (Fig. 6A). In parallel, VEGFα, BMP2, and BMP7 were adopted to maintain the odontogenic potential of mDMCs. Although well-structured tooth was not observed, dentin-like structures were present (Fig. 6D). Together, VEGFα, BMP2, and BMP7 promoted the expression of dental mesenchyme-specific genes and the formation of dentin-like structures.

DISCUSSION
Numerous signaling factors in dental epithelium and/or mesenchyme interact with each other to regulate odontogenesis and induce the expression of dental mesenchyme-specific transcription factors during tooth development (Thesleff, Vaahtokari & Partanen, 1995). In the present study, we found that cultured mDMCs lost the odontogenic potential and failed to initiate tooth formation. Consistently, the dental mesenchyme-specific transcription factors (Msx1, Pax9, and Lhx6) were decreased in cultured mDMCs, and the mutation of these transcription factors was shown to cause tooth agenesis disorders (Peters et al., 1998;Satokata & Maas, 1994;Zhao et al., 2013). The loss of odontogenic potential and decreased expression of dental mesenchyme-specific genes may be due to the absence of inductive signals from the dental epithelium or mesenchyme. Since Bmp and Fgf signaling pathways mediate the odontogenic inductive signals of dental mesenchyme (Liu et al., 2013;Thesleff, Vaahtokari & Partanen, 1995), the expression of representative components within these two signaling families was analyzed in mDMCs. The expression of Bmp4 shifts from dental epithelium to mesenchyme, which is concomitant with the shift of instructive potential during tooth development (Vainio et al., 1993), but the expression of Bmp4 in the present study was not significantly changed when mDMCs were cultured in vitro. This suggests that Bmp4 in cultured mDMCs is not sufficient to substitute for the odontogenic potential and that other factors besides Bmp4 are responsible for the inductive potential. Similarly, although Fgf10 stimulates cell proliferation in the dental epithelium during tooth morphogenesis (Kettunen et al., 2000), the mRNA level of Fgf10 was comparable between freshly-isolated and cultured mDMCs. In contrast, the expression of Fgf3 and Bmp2 in cultured mDMCs was significantly reduced. Fgf3 regulates the number, position, and interrelation of cusps in molar teeth; mutation of the Fgf3 gene leads to dosage-dependent morphological changes in teeth of both mice and human patients (Charles et al., 2009). Bmp2 is essential for the differentiation of ameloblasts and odontoblasts (Guo et al., 2015;Yang et al., 2012). Thus, the downregulation of these genes may account for the loss of odontogenic potential in cultured mDMCs. However, Bmp2 was expressed at a relatively low level in E14.5 mDMCs, and Fgf3 knockout mice do not exhibit any overt defects in teeth (Mansour, Goddard & Capecchi, 1993), raising the possibility that the loss of odontogenic potential in cultured mDMCs may also involve other genes.
Transcriptome analysis, as conducted in this work, has provided insight into the molecular mechanism underlying the loss of odontogenic potential. The transcriptomic profiles reflected the potential of mDMCs, and the mDMCs without odontogenic potential were strikingly separated from those with odontogenic potential. Moreover, the activities of Bdnf, Vegf α, Bmp2, and Bmp7 were predicted to be inhibited. These growth factors were reported to be involved in tooth development. Bdnf and its receptor Ntrk2 are involved in the MAPK pathway and play roles in epithelial-mesenchymal interactions in early tooth morphogenetic events (Nosrat et al., 1997). Vegf α is an important factor that induces angiogenesis and related with the MAPK signaling pathway. It is expressed in inner enamel epithelial cells and the basement membrane (Aida et al., 2005;Miwa et al., 2007), indicating a potential role in the epithelial-mesenchymal interactions. Bmp2 promotes the maturation of odontoblasts and Bmp2 conditional knockout mice display abnormal tooth phenotypes with a hypomineralization enamel layer, delayed odontoblast differentiation, abnormal dentin tubules, and decreased tooth-related gene expression (Guo et al., 2015;Yang et al., 2012). Bmp7 is also essential for tooth development and its level in the fetal dental mesenchymal cells is higher compared with that in the adult dental pulp cells, representing a potential mediator of the inductive odontogenic potential of dental mesenchyme (Gao et al., 2015). Consistently, we found that VEGFα, BMP2, and BMP7 increased the expression of dental mesenchyme-specific genes. Even though the supplementation with these growth factors did not restore the odontogenic potential of mDMCs, dentin-like structures formed instead of amorphous matrix, suggesting their potential roles in the maintenance of odontogenic potential. Recently, immortalized fetal dental mesenchymal cell lines were established (Huang et al., 2015;Wu et al., 2015), and a medium supplemented with upstream signaling molecules such as Vegf α, Bmp2, and Bmp7 may preserve the biological properties of mDMCs.
Although the effects of Igf2, Fgf10, and Tgfb1 were predicted to be enhanced in cultured mDMCs, they are well-known mesenchymal signals that mediate epithelialmesenchymal interactions during tooth development (Matsumoto et al., 2011;Nakao et al., 2013;Vaahtokari, Vainio & Thesleff, 1991). Interestingly, the level of Igf2 in dental mesenchyme decreased over time during development because of the hypermethylation of CpG islands (Khan et al., 2012); this suggests that the inhibition of Igf2 activity is required for normal tooth development. However, further studies are still needed to investigate whether the overactivation of these factors leads to abnormal phenotype of teeth, and whether inhibitors of these factors play a role in the maintenance of odontogenic potential.
In the present study, supplementation with Vegf, Bmp2, and Bmp7 did not completely restored the odontogenic potential of mDMCs. However, the GO analysis also provided a clue for the establishment of culture system for mDMCs. GO analysis showed that multiple biological processes involving the development of multiple organs were dysregulated when mDMCs were cultured in vitro. Specifically, Chromatin modifier enzymes play essential roles in the establishment of transcriptional programs accompanying cell differentiation during tooth development (Brook, 2009). Mesenchymal cell migration and compaction are required to induce the expression of critical odontogenic genes and the differentiation of odontoblasts (Hu, Parker & Wright, 2015;Mammoto et al., 2011). The dysregulation of cell motion also contributed to the compromised potential in cultured mDMCs. Since both the mandible and teeth are mineralized tissue derived from the neural crest (Chai et al., 2000), multiple genes acting in skeletal system development have been identified as affecting the odontogenic signaling cascades (Komori, 2006;Nakashima et al., 2002). Thus, growth factors or cytokines that related with transcriptional regulation, cell differentiation or mineralization, and cell motion may facilitate the maintenance of odontogenic potential.
In conclusion, we assessed the odontogenic potential of in vitro-expanded mDMCs and conducted an analysis of their transcriptome profiles. The results revealed that the transition to an in vitro setting induced the loss of odontogenic potential and a significant modulation of the overall transcriptome. Genes encoding a wide array of components of MAPK, TGF-β/BMP, and Wnt pathways were significantly downregulated in cultured cells. Our results also indicated that inhibited activities of Vegf α, Bmp2, and Bmp7 contributed to the loss of odontogenic potential and supplementation with these growth factors played roles in the maintenance of odontogenic potential in mDMCs.