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Preliminary Observation on the Cytotoxic Activity of New Chlorophyllin Derivative RCD on Human Tumour Cell Lines In Vitro

Background: Chlorophyllin is used in traditional Chinese medicine because of its anticancer properties. This article describes the preparation and cytotoxic activity of a reduced chlorophyllin derivative (RCD) on tumour cell lines. Materials and Methods: RCD was prepared by reducing chlorophyllin with lithium aluminium hydride, and its solubility in the alkaline and organic phases are different from that of the parent compound chlorophyllin. Results: RCD also has different chromatographic behaviour from chlorophyllin on thin-layer chromatography and high-pressure liquid chromatography, and excitation and emission spectra. RCD has cytotoxic activity against ZR-75, MCF-7 and HT-29, human tumour cell lines. A clonogenic assay showed that the growth of tumour colonies on soft agar was reduced by RCD in a dose-dependent manner. Conclusion: RCD is a novel compound exhibiting anticancer activity.