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Claudin 9 overexpression increases migration potential and proliferation rate of human gastric adenocarcinoma cells

Claudin family proteins play an important role in the barrier function of tight junctions (TJ), preserving cell polarity and determining the size and charge of the molecules that pass trough the paracelular space in epithelial and endothelial tissues. Claudin (CLDN) expression is altered in several tumors, and our group has recently reported that CLDN 6, 7 and 9 are overexpressed in human gastric adenocarcinomas, however the role of these CLDNs in tumor progression is unknown. To determine if CLDN overexpression confere advantages to AGS cells, like increased proliferation rate, apoptosis evasion and tissue invasion, we evaluated the invasive capability of CLDNs transfected cells in Matrigel invasion chambers and by wound‐healing scratch assay. Proliferation rate by Brdu incorporation, and apoptosis of TNF treated cells by PARP detection were also measured. Overexpression of the three CLDNs resulted in increased migration potential, however CLDN 9 transfected cells migrated faster than the others. CLDN 9 overexrpession also caused increased proliferation rate of AGS cells.