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[Effects on T cell proliferation by culture supernatant of fibroblast cells modified with mIDO gene].

AIM
To investigate the effects on T cell proliferation by culture supernatant of fibroblast cells modified by mIDO gene.


METHODS
Eukaryotic expression plasmid pEGFP-Nl-mlDO was transfected into the mouse fibroblast cell lines by liposome. The concentration of tryptophan in pEGFP-Nl-mIDO fibroblast cell culture medium was tested. The effects on ConA-stimulated T cells, proliferation by pEGFP-Nl-mlDO fibroblast cell culture medium were analyzed by CFSE technique.


RESULTS
pEGFP-N1-mlDO was transfected into the fibroblasts cell lines successfully. Tryptophan in pEGFP-Nl-mIDO-transfected-fibroblast cell culture medium was undetectable, which showed pEGFP-Nl-mIDO-transfected-fibroblast cells produced active mIDO, resulting in the degradation of tryptophan in the medium. mIDO could inhibit proliferation of ConA-stimulated T cells, which was marked by CFSE.


CONCLUSION
T cell proliferation can be inhibited by the fibroblast cells modified by mIDO gene.