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NIR responsive liposomal system for rapid release of drugs in cancer therapy

To design a rapid release liposomal system for cancer therapy, a NIR responsive bubble-generating thermosensitive liposome (BTSL) system combined with photothermal agent (Cypate), doxorubicin (DOX), and NH4HCO3 was developed. Cypate/DOX-BTSL exhibited a good aqueous stability, photostability, and photothermal effect. In vitro release suggested that the amounts of DOX released from BTSL were obviously higher than that of (NH4)2SO4 liposomes at 42°C. After NIR irradiation, the hyperthermic temperature induced by Cypate led to the decomposition of NH4HCO3 and the generation of a large number of CO2 bubbles, triggering a rapid release of drugs. Confocal laser scanning microscope and acridine orange staining indicated that Cypate/DOX-BTSL upon irradiation could facilitate to disrupt the lysosomal membranes and realize endolysosomal escape into cytosol, improving the intracellular uptake of DOX clearly. MTT and trypan blue staining implied that the cell damage of Cypate/DOX-BTSL with NIR irradiation was more severe than that in the groups without irradiation. In vivo results indicated that Cypate/DOX-BTSL with irradiation could dramatically increase the accumulation of DOX in tumor, inhibit tumor growth, and reduce systemic side effects of DOX. These data demonstrated that Cypate/DOX-BTSL has the potential to be used as a NIR responsive liposomal system for a rapid release of drugs in thermochemotherapy.


Introduction
Chemotherapy is still a major treatment modality for tumors, yet its efficacy is limited by the serious side effects on normal tissues. [1][2][3][4] Photothermal therapy (PTT) has been considered as a noninvasive method of cancer therapy, which uses photothermal agents to generate heat under photoirradiation without injuring healthy tissues. 5,6 Although PTT is highly effective in inhibiting tumor growth, it remains a major challenge to achieve tumor eradication without tumor regrowth. A combination therapy of PTT and chemotherapy has been considered as a promising strategy to improve therapeutic efficiency and minimize side effects. Nevertheless, to realize the maximal synergistic effect, the accurate doses of chemotherapy drug and photothermal agent should be simultaneously delivered to the same tumor cells.
Nanodrug delivery systems such as liposomes and nanoparticles have been developed to selectively accumulate in the tumor compartment via a passive targeting mechanism named the enhanced permeability and retention (EPR) effect, 7,8 which can reduce potential toxicity and enhance effective therapy. PEGylated liposomes are recognized as stable vehicles of drugs; however, the liposomes need to be triggered to release the drugs after they are accumulated in the tumor cells for effective therapy. Thermosensitive liposomes can immediately be triggered for the rapid release of antitumor drugs by changing the permeability of liposomes at hyperthermic temperature (40°C-42°C) in tumor sites. 9,10 Recently, a bubble-generating thermosensitive liposome (BTSL) system that contained ammonium bicarbonate (NH 4 HCO 3 ) has been developed to facilitate the rapid release of drugs. The key to this liposome system is that NH 4 HCO 3 can generate CO 2 bubbles at a local temperature of ~42°C, and then the bubbles collapse to create permeable defects in the lipid bilayer and induce a rapid drug release. 11,12 On the other hand, the lysosomal membranes are disrupted by the collapse of CO 2 bubbles, which not only facilitate endolysosomal escape of drugs into cytosol but also release proteases into the cytosol to cause cell death. [13][14][15][16] Although hyperthermia can induce the drugs to release from BTSL, photothermal effect from photothermal agents upon photoirradiation seems to be a better triggered release method. Under NIR irradiation, photothermal agents can raise the temperature to 42°C and induce the decomposition of NH 4 HCO 3 to generate CO 2 bubbles, which then cause the rapid drug release. Additionally, this novel BTSL system containing antitumor drugs and photothermal agents can enhance the synergistic effect of chemotherapy and PTT. Nevertheless, a study on the application of BTSL for the multimodal therapy has seldom been done so far.
The aim of this work was to develop a NIR responsive liposomal system (Cypate/doxorubicin [DOX]-BTSL) for rapid release of drugs in synergistic therapy of PTT and chemotherapy. As shown in Figure 1, Cypate and DOX are encapsulated into the BTSL system containing NH 4 HCO 3 first. At the tumor site, the liposomes suffer from NIR irradiation, which leads to a hyperthermic temperature and PTT. When the temperature is increased to 40°C-42°C, the BTSLs generate CO 2 bubbles due to the decomposition of NH 4 HCO 3 , inducing a rapid release of drugs, enhancing the synergistic therapeutic efficacy of chemotherapy and PTT. The physicochemical characteristics and release features of the liposomes were investigated to evaluate the potential to be used as a NIR responsive liposomal system for a rapid release of drugs. Moreover, photothermal effect, cellular uptake, tumor accumulation, and antitumor activity in vivo of the liposomes were assayed to demonstrate the effectiveness of this rapid drug release system for synergistic therapy of PTT and chemotherapy. Figure 1 schematic illustration of BTsl integrating cypate dye and DOX for synergistic therapy of PTT and chemotherapy. Note: When the temperature is heated to 40°c-42°c due to NIr laser irradiation, the BTsl generate cO 2 bubbles, inducing a rapid release of drugs.

Preparation of liposomes
Liposomes were prepared by thin-film hydration, followed by sonication method. [23][24][25][26] In brief, DPPC, Chol, DSPE-PEG 2000 , and Cypate at a molar ratio of 12:8:1:0.8 were dissolved in a mixed solvent of chloroform and methanol (V:V =1:1) and placed in a high vacuum to remove the residual organic solvent. The lipid film was then hydrated using an aqueous (NH 4 ) 2 SO 4 (350 mM) or NH 4 HCO 3 (2.7 M) solution via sonication at ice bath conditions. The free (NH 4 ) 2 SO 4 or NH 4 HCO 3 was removed by dialysis against 10 wt% sucrose solution with 5 mM NaCl. Free Cypate was removed using dialysis and centrifugation. DOX was added to the liposome solution at a drug/lipid ratio of 0.1 (w/w) and incubated at room temperature for 24 h. Free DOX was removed using dialysis in distilled water for 9 h. (NH 4 ) 2 SO 4 liposomes were abridged for L. The composition of the liposomes is listed in Table 1.

characterization of liposomes
The mean diameter, polydispersity index (PDI), and zeta potential were measured by dynamic light scattering (DLS; Zetasizer Nano-ZS, Malvern Instruments Ltd., Worcestershire, UK). The encapsulated DOX concentration was determined by fluorescence spectrophotometer (F-7000; Hitachi, Tokyo, Japan) after destroying the liposomes with 10% Triton X-100. The encapsulation efficiency (EE) and drug loading (DL) content of DOX were calculated according to the following equation:

Photothermal effect
The solution of Cypate/DOX-BTSL and free Cypate (each 2.5 mL) with various concentrations of Cypate was irradiated at the excitation wavelength of 785 nm (2.8 W/cm 2 ) for 5 min. The temperature of the solution was recorded for each 30 s. PBS was used as the control in this experiment.

In vitro release of DOX from liposomes
The drug release behavior of DOX from the liposomes was observed by immersing the test liposomes (10 mg/mL, 2 mL) in tube containing 10 mL PBS (pH 7.4 or 5.0), which were performed at 37°C or 42°C in an air-bath shaker at 100 rpm. 27 In addition, DOX release behavior from liposomes with or without NIR irradiation (785 nm, 2.8 W/cm 2 ) for 10 min at initial time was also investigated to assess the photothermal-induced drug release. Samples of 2.0 mL were taken and replaced with an equal volume of fresh PBS at the definite time intervals. The amount of DOX released was determined using a fluorescence spectrometer (Ex =485 nm, Em =580 nm) at each time intervals. acridine orange (aO) staining AO fluorescent dyes were further used to assess the disruption of lysosomal membranes in MCF-7 cells. 15 MCF-7 cells were seeded at a density of 2×10 5 cells/well at a 12-well plate and then incubated overnight at 37°C with 5% CO 2 . After treated with Cypate/DOX-L and Cypate/DOX-BTSL at the Cypate concentrations of 0.5, 1, and 2 μg/mL for 4 h, the cells were exposed to photoirradiation for 10 min at 780 nm, 2.8 W/cm 2 . The cells treated with PBS were as a control. After 2 h, the cells were washed with PBS and stained with 200 μL AO (100 μg/mL) for 15 min in dark and then immediately washed three times with PBS. Finally, the cells were observed using fluorescence microscopy (IX71-F22PH; Olympus) with an excitation wavelength of 488 nm.

MTT assay
Cytotoxicity of the liposomes with or without NIR laser irradiation was assessed by a MTT method. MCF-7 cells were seeded into a 96-well plate at a density of 1×10 5 cells/well and then incubated overnight at 37°C with 5% CO 2 . The cells were incubated with free DOX, Cypate/DOX-L, or Cypate/ DOX-BTSL for 4 h at various DOX concentrations of 20, 40, 60, 80, and 100 μg/mL, followed by 10 min NIR irradiation (785 nm, 2.8 W/cm 2 ). The formulation without NIR irradiation was used as a control. After the further incubation for 2 h, the cells were stained with 20 μL MTT solution (5.0 mg/mL) for 4 h, and the formed formazan crystals was dissolved with 150 μL DMSO. Absorbance was recorded at 570 nm using a microplate reader (Infinite M200 Pro; Tecan, Männedorf, Switzerland).

Trypan blue staining
The effect of Cypate/DOX-L and Cypate/DOX-BTSL on cell injury was also evaluated using trypan blue exclusion method. 15  In vivo DOX accumulation The nude mice were chosen for the experiment when the tumor volume reached 80-100 mm 3 . The mice were randomly divided into three groups (n=4 per group), and then the mice were treated with free DOX, Cypate/DOX-L, and Cypate/ DOX-BTSL (all containing 10 mg/kg DOX) via intratumoral injection, respectively. Tumor sites were irradiated with NIR for 5 min (785 nm, 1.6 W/cm 2 ) after administration of Cypate/DOX-L and Cypate/DOX-BTSL for 2 h. Then, the mice were imaged using a CRI Maestro system (Maestro™ 2 Maestro™ EX) at 1, 2, 3, and 4 d postinjection. The average fluorescent intensity at tumor was calculated at different timings. Moreover, the mice were sacrificed at 24 h after injection, and various organs including heart, liver, spleen, lung, kidney, and tumor were collected and imaged to monitor the in vivo DOX accumulation. 30 Antitumor efficacy study To evaluate the antitumor efficacy for each treatment, 24 tumor-bearing mice were randomly divided into six groups. The mice were treated with no treatment, free DOX, Cypate/ DOX-L (with and without laser), and Cypate/DOX-BTSL (with and without laser) at 10 mg/kg DOX via intratumoral injection. For the laser treatment groups, the tumor areas were exposed to photoirradiation for 5 min at the wavelength of 785 nm (1.6 W/cm 2 ) at 1 d.

statistical analysis
All experiment data were expressed as mean ± standard deviation (SD) and analyzed statistically by an one-way analysis of variance. The level of significance was defined at P,0.05.

Results and discussion Preparation and characterization of liposomes
To trigger a rapid release of drugs in tumor cells, a NIR responsive liposomal system was fabricated by thin-lipid film hydration and sonication. The key components of this new system were Cypate and NH 4 HCO 3 . Cypate exhibits NIRF and PTT after excitation by near-infrared light, which can trigger hyperthermia. 31 Upon heating to a high temperature (40°C-42°C), NH 4 HCO 3 encapsulated in the liposomes could quickly decompose to generate CO 2 bubbles, leading to permeable defects in the lipid bilayers of the liposomes, facilitating the rapid local release of drugs ( Figure 1). Based on the transmembrane gradient of (NH 4 ) 2 SO 4 or NH 4 HCO 3 , DOX was loaded into the test liposomes. Table 2 lists the characteristics of DOX-L, DOX-BTSL, Cypate/ DOX-L, and Cypate/DOX-BTSL. The mean diameters of DOX-L and DOX-BTSL were 133.6±2.8 and 126.7±5.2 nm, and that of Cypate/DOX-L and Cypate/DOX-BTSL were 156.6±2.0 and 156±3.2 nm. The PDIs of the liposomes were all ,0.200. These data showed a good homogeneity of the liposomes. The liposomes with these sizes could be internalized by tumor cells and accumulated in the deeper areas of tumor sites for cancer therapy by the EPR effect. 32,33 The zeta potentials of liposomes were obviously negative in distilled water (Table 2), which suggested that these liposomal systems would be stable in aqueous media. 34 Besides, as the crucial element for clinical application, an efficient loading of DOX into the aqueous phase of each formulation (84.5%) was achieved, and the DL contents were all 6.93%.

aqueous stability, photostability, and photothermal effect
Cypate is a dicarboxylic acid-containing carbocyanine fluorophore with an excellent molar extinction coefficient and high photothermal conversion efficiency. The aqueous stability of Cypate was a prerequisite for cancer therapy; thus, we evaluated the aqueous stability of Cypate/DOX-BTSL and free Cypate solution. Figure 2A and B shows that the liposomes exhibit a good stability of Cypate in the fluorescence and UV absorbance at pH 5.0 and pH 7.4, compared to free Cypate. It indicated that the liposomes could reduce the photobleaching of Cypate and maintain a stable structure in aqueous solution at acidic and physiological pH, leading to the prevention of Cypate from the degradation in acidic endocytic compartments at tumor due to its efficient encapsulation in the liposomes. 35 As shown in Figure 2C, the average diameters of Cypate/DOX-BTSL and Cypate/DOX-L remain around the initial sizes of liposomes without aggregation and precipitation in 10 d, suggesting a great stability of Cypate/ DOX-BTSL and Cypate/DOX-L.
The photostability of liposomes and free Cypate was also demonstrated since it significantly affected the efficiency of PTT. According to the UV spectra of Cypate/DOX-BTSL, Cypate/DOX-L, and free Cypate ( Figure 2D), the absorbance of free Cypate decreased quickly under 785 nm photoirradiation within 3 min due to the rapid photobleaching. Nevertheless, Cypate in the liposomes had much slower decrease of the absorbance under the same condition during 10 min. It implied that the liposomes could improve the photostability of Cypate and provide more efficient PTT during longer irradiation time as compared to free Cypate.
To assess the photothermal effect of Cypate/DOX-BTSL and free Cypate, their thermal behaviors at different concentrations of Cypate in PBS (pH 7.4) under NIR irradiation were Notes: a The diameter, PDI, and zeta potential (mean ± sD) measured by Dls three times. b ee and Dl content of DOX (mean ± SD) determined by fluorescence spectrometer three times.

4231
NIr responsive liposomal system measured. At the concentration of 2.0 μg/mL Cypate, free Cypate, and Cypate/DOX-BTSL all displayed an obvious increase in the temperature from 31°C to 42°C with the NIR irradiation (2.8 W/cm 2 ) for 180 s, whereas PBS as a control did not show any increase in temperature ( Figure 2E and F). It suggested that Cypate/DOX-BTSL could quickly trigger hyperthermia at a low concentration of Cypate within a short photoirradiation, which was similar to the result of free Cypate. Moreover, it showed that the higher concentration of Cypate could cause more significant hyperthermia. These results indicated that Cypate/DOX-BTSL exhibited a concentrationdependent photothermal effect and had a potential to trigger photothermal damage against tumor cells (42°C). 36,37 In vitro release of DOX from liposomes To access the effect of NH 4 HCO 3 on the rapid release of drugs from liposomes, the release profiles of DOX from DOX-BTSL and DOX-L were evaluated at 37°C (physiological temperature) and 42°C (hyperthermic temperature), respectively. As shown in Figure 3A, the amounts of DOX released from DOX-L and DOX-BTSL are 20%-26% in PBS (pH 5.0 or 7.4) at 37°C during 24 h, suggesting that the release rate of DOX was rather slow and the liposomes were all relatively stable at physiological temperature in pH 5.0 and 7.4. The DOX release rate of DOX-L at 42°C ( Figure 3B) was similar to that at 37°C, which indicated that its drug release was not affected by temperature. However, the amounts of DOX released from DOX-BTSL were 84% (pH 5.0) and 50% (pH 7.4) within 30 min at 42°C, which was obviously higher than that from DOX-L, implying that the release of DOX-BTSL had a temperature-dependent characteristic due to NH 4 HCO 3 . The reason of the rapid release was that the decomposition of NH 4 HCO 3 at 42°C generated a large number of CO 2 bubbles, which grew rapidly and then collapsed violently, ultimately resulting in a cavitation force that could perforate the lipidbilayer membranes and trigger a significant release of drugs. 11 Furthermore, at pH 5.0, corresponding to the pH values of lysosome, DOX-BTSL displayed the faster drug release rate than that at pH 7.4 at 42°C, indicating that the release from DOX-BTSL exhibited a pH-sensitive drug release property and facilitated the rapid release of DOX in the lysosome system at hyperthermic temperature.
In this study, the effect of laser irradiation on drug release behavior from Cypate/DOX-BTSL was also investigated. After laser irradiation, the total DOX released from Cypate/ DOX-BTSL was 88% at pH 5.0 and 56% at pH 7.4 during 10 min ( Figure 3C). It suggested that the NIR irradiation could trigger the rapid drug release from Cypate/DOX-BTSL and the release had a pH-sensitive property. The reason of this photothermal-induced rapid release of drugs was that Cypate encapsulated in liposomes could trigger the hyperthermia under photoirradiation, inducing the decomposition of NH 4 HCO 3 and the generation of CO 2 bubbles.
Therefore, it could be concluded that Cypate/DOX-BTSL had photothermal-induced and pH-sensitive drug release properties. BTSL exhibited a good stability at 37°C during blood circulation. However, when the hyperthermia (42°C) was reached by NIR irradiation or local heating, the drug release would be extremely fast, causing a high drug concentration gradient to facilitate the diffusion of drug into the tumor cells. The fast-triggered drug release also acquired the ability to counteract rapid passage of the bloodstream and prevent the liposomes from washout from the tumors. 38 In vitro cellular uptake The cellular uptake of free DOX, Cypate/DOX-L, and Cypate/DOX-BTSL was investigated by CLSM. It has been known that free DOX molecules are transported into the cells through a passive diffusion pathway, whereas liposomes are taken up by cells via an endocytotic pathway and then escape from the endosomes and/or the lysosomes to enter the cytoplasm. 39 As shown in Figure 4, free DOX and DOX released from Cypate/DOX-L and Cypate/DOX-BTSL are all localized in the nucleus after 4 h incubation. This demonstrated that DOX has been released from liposomes and accumulated in the nucleus after 4 h. Figure 4 exhibits that the group of Cypate/DOX-BTSL with NIR irradiation has the strongest red fluorescence in the nucleus, compared to those of Cypate/DOX-BTSL without NIR irradiation and Cypate/DOX-L, which was approximately to free DOX. However, red fluorescence distribution had no significant difference between Cypate/DOX-L group with and without irradiation. These data indicated that Cypate/DOX-BTSL with NIR irradiation improved the intracellular uptake of DOX clearly. It was due to the photothermal-induced rapid release of DOX from Cypate/DOX-BTSL activated by the formation of CO 2 bubbles via the thermal decomposition of the encapsulated NH 4 HCO 3 , which was consistent with the results of in vitro release. lysosomes. To investigate the disruption of lysosomal membranes, we monitored the integrity of lysosomes with photoirradiation using AO as an intracellular indicator. AO is a metachromatic fluorophore that becomes charged and is retained by proton trapping within acidic compartments.

Disruption of lysosomal membranes
AO emits an intense red fluorescence in intact acidic lysosomes and displays a green fluorescence in neutralized nuclei and cytosol. When acidic compartment such as lysosomes is disrupted, the red fluorescence disappears and only the green fluorescence can be observed. 40    cytosol, which was attributed to photothermal cytotoxicity. In addition, compared to Cypate/DOX-L with irradiation, the red fluorescence obviously decreased in Cypate/DOX-BTSL laser group at the same concentration of Cypate. The red fluorescence significantly decreased in the presence of Cypate/DOX-BTSL (0.5 μg/mL Cypate) with irradiation and mostly disappeared at the Cypate concentration of 2 μg/mL. It suggested that Cypate/DOX-BTSL at a low concentration of Cypate could effectively damage the lysosomal membranes under irradiation. It indicated that the disruption of lysosomal membranes was owing to not only photothermal effect from Cypate but also the collapse of the CO 2 bubbles. 11 These could facilitate the DOX release to nucleus and then enhance the synergistic cytotoxicity of chemotherapy and PTT.

Photothermal cytotoxicity
The photothermal injury of Cypate/DOX-L and Cypate/ DOX-BTSL on MCF-7 cells was investigated by MTT. Figure 6 displays cell viability of MCF-7 cells treated with free DOX, Cypate/DOX-L, and Cypate/DOX-BTSL at various concentrations of DOX with or without NIR irradiation. With the increase in DOX concentration, the cell viability of all groups without irradiation was decreased since the cytotoxicity was improved due to chemotherapy. The cell viability of Cypate/DOX-L and Cypate/DOX-BTSL groups with irradiation was much lower than that of groups without irradiation. It was known that free Cypate did not induce cell death without photoirradiation, but it could kill the cells with irradiation owing to photothermal effect. Therefore, the explanation of the high cytotoxicity of the laser groups was that Cypate encapsulated in the liposomes could induce hyperthermia (42°C), resulting in severe photothermal damage on tumor cells. Moreover, it was seen that the Cypate/ DOX-BTSL groups with irradiation had the lowest cell viability. It was since the hyperthermia triggered the rapid release of DOX from the bubble liposomes, increasing the intracellular concentration of DOX, causing stronger growth inhibition effect on the cells. The photothermal injury of Cypate/DOX-L and Cypate/ DOX-BTSL on MCF-7 cells was further validated by trypan blue staining. Trypan blue dye molecules can easily penetrate plasma membranes of dead cells to stain the nuclei, whereas viable cells cannot be stained. From Figure 7, the cell damage of Cypate/DOX-L and Cypate/DOX-BTSL with NIR irradiation is more severe than the groups without irradiation, and the photothermal injury of the laser groups increased clearly with the increase in the concentrations of Cypate. Moreover, the photothermal injury of Cypate/DOX-BTSL under irradiation was more obvious than Cypate/DOX-L laser group. PBS as a control exhibited no damage on cells with or without laser irradiation. These data indicated that the cells suffered from severe photothermal injury from Cypate/DOX-BTSL because of the bubbles, which were consistent with the result of MTT.
Based on the results of MTT and trypan blue staining, synergistic cytotoxicity of Cypate/DOX-BTSL was due to a combination of chemotherapy and PTT. Notably, the hyperthermia from Cypate not only led to PTT but also induced the rapid release of DOX from the bubble liposomes, enhancing the cytotoxicity significantly.

In vivo DOX accumulation
The aforementioned experimental results in vitro indicated that Cypate/DOX-BTSL with NIR irradiation improved the intracellular uptake of DOX clearly. The accumulation of DOX in tumor and organs with or without NIR laser irradiation was studied following an intratumoral injection of free DOX, Cypate/DOX-L, and Cypate/DOX-BTSL. According to  Figure 8A, the fluorescent images of major organs and tumors ex vivo indicated that high drug concentration spread in lung and tumor at 24 h postinjection of free DOX, whereas Cypate/ DOX-L and Cypate/DOX-BTSL with laser irradiation presented high drug concentration only in tumor. It suggested that these liposomes could improve nonspecific accumulation of DOX and enrich the DOX in tumor owing to the EPR effect. The accumulations of DOX in tumor were further investigated at 1, 2, 3, and 4 d postinjection by in vivo imaging ( Figure 8B and C). In the laser treatment groups, the fluorescence intensities of DOX in tumor were stronger than that of free DOX group during the imaging period of 4 d, and the DOX from Cypate/DOX-L and Cypate/DOX-BTSL with irradiation was significantly retained around the tumor even after 4 d, which was attributed to the prolonged retention time of DOX in tumor by EPR effects of liposomes. 41 Compared to Cypate/DOX-L laser group, the Cypate/DOX-BTSL laser group exhibited stronger fluorescence intensity of DOX in tumor on the same condition. These results indicated that Cypate/DOX-BTSL dramatically increased the amount of DOX accumulated in tumor due to the photothermal-induced rapid release of DOX. Therefore, Cypate/DOX-BTSL would facilitate to prolong the retention of DOX in tumor, reduce systemic side effects, and enhance therapeutic efficacy of DOX. The in vivo antitumor efficacy of Cypate/DOX-L and Cypate/DOX-BTSL in a synergistic therapy of PTT and chemotherapy was elucidated by the nude mice-bearing 4T1 tumors after treatment. From Figure 9A, the tumor volume of the mice with no treatment as control rapidly increases in 14 d, while the tumor growth for free DOX and the liposome groups (with or without laser irradiation) is suppressed. For Cypate/DOX-L and Cypate/DOX-BTSL, the laser irradiation group had a better antitumor efficacy than the relative group without irradiation, implying that the effect of local photothermal heating could not be negligible. In the laser groups, Cypate/DOX-BTSL suppressed tumor growth more significantly than Cypate/DOX-L, since considerably more DOX molecules were released and accumulated in tumor due to the photothermal-induced rapid release of DOX from Cypate/DOX-BTSL. It was consistent with the previous results of in vitro release, cellular uptake, and in vivo imaging. Furthermore, the body weight was measured to evaluate treatment-induced toxicity ( Figure 9B). The mice treated with free DOX lost 28% weight at 14 d, indicating the serious toxic side effects of free DOX. 32 The mice treated with the liposomes lost less origin weight and had a lower toxicity than the free DOX group. Notably, there was no significant reduction in body weight of the mice in the laser groups, implying the excellent safety of these liposomes under irradiation. These findings suggested that the synergistic therapy of PTT and chemotherapy of these liposomes could effectively inhibit tumor growth and reduce side effects of DOX, achieved a promising strategy for antitumor therapy.

Conclusion
A NIR responsive bubble-generating liposome (Cypate/ DOX-BTSL) was fabricated with NH 4 HCO 3 . Cypate/DOX-BTSL exhibited a good aqueous stability, photostability, and photothermal effect. The liposomes had a property of photothermal-induced rapid drug release due to the hyperthermia induced by Cypate and CO 2 bubbles triggered by NH 4 HCO 3 . After NIR irradiation, Cypate/DOX-BTSL could enhance the intracellular uptake and tumor accumulation of DOX significantly, compared to the groups without NH 4 HCO 3 . Therefore, this NIR responsive liposomal system shows a great perspective as a rapid drug release system for synergistic therapy of PTT and chemotherapy.