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Biosynthesis of lipovitellin by the crustacean ovary.

The in vitro incorporation of 3H-amino acids into purified lipovitellin by cultured crustacean ovaries can be demonstrated using either density gradient centrifugation or preparative electrophoresis as a final purification step. In addition 3H-glycerol is incorporated into the phosphatidyl choline moiety of the lipovitellin. This evidence strongly suggests that the ovary is capable of synthesizing both the lipid and the protein components of the lipovitellin and therefore may not incorporate such moieties intact from the circulating hemolymph.