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Optimized Induction of Neurospheres from Porcine Mesenchymal Stem Cells

Neurospheres are free‐floating clusters of cells that provide researchers a unique method to investigate neural stem cells. Stem cells typically grow as an adherent monolayer on the culture surface, however, when introduced to proper media and growth factors without adherent substrates, these stem cells will form non‐adherent three‐dimensional aggregates called neurospheres. Induction of neurospheres increases multipotency and regenerative capacity of some stem cells, such as adipose‐derived mesenchymal stem cells (adMSCs). Currently, there are no standardized methods for neurosphere induction and evaluation which limits their utility. Studies into cancer, neural development, and regenerative medicine would all benefit from an optimized neurosphere induction protocol. We aimed to establish an optimized protocol for inducing neurospheres from adMSCs in an efficient and reproducible manner.