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Hydrogen sulfide stimulates β-amylase activity during early stages of wheat grain germination

We recently reported that H2S could significantly promote the germination of wheat grains subjected to aluminum (Al3+) stress.1 In these experiments seeds were pretreated with the H2S donor NaHS for 12 h prior to Al3+ stress. During this pre-incubation period we observed that H2S increased the activity of grain amylase in the absence of Al3+. Using embryoless half grains of wheat we now show that H2S preferentially affects the activity of endosperm β-amylase and that α-amylase synthesis and activity is unaffected by this treatment.