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Ultrasound-based artificial intelligence in gastroenterology and hepatology

Artificial intelligence (AI), especially deep learning, is gaining extensive attention for its excellent performance in medical image analysis. It can automatically make a quantitative assessment of complex medical images and help doctors to make more accurate diagnoses. In recent years, AI based on ultrasound has been shown to be very helpful in diffuse liver diseases and focal liver lesions, such as analyzing the severity of nonalcoholic fatty liver and the stage of liver fibrosis, identifying benign and malignant liver lesions, predicting the microvascular invasion of hepatocellular carcinoma, curative transarterial chemoembolization effect, and prognoses after thermal ablation. Moreover, AI based on endoscopic ultrasonography has been applied in some gastrointestinal diseases, such as distinguishing gastric mesenchymal tumors, detection of pancreatic cancer and intraductal papillary mucinous neoplasms, and predicting the preoperative tumor deposits in rectal cancer. This review focused on the basic technical knowledge about AI and the clinical application of AI in ultrasound of liver and gastroenterology diseases. Lastly, we discuss the challenges and future perspectives of AI.


INTRODUCTION
Liver disease causes two million deaths per year in the world among which cirrhosis is the 11 th leading cause of death in the world and liver cancer is the 16 th leading cause of death [1]. The prevalence of nonalcoholic fatty liver disease (NAFLD) is 25.0% and is estimated to be 33.5% by 2030 [2]. Gastrointestinal diseases affect an estimated 60 to 70 million American citizens annually. It is reported that pancreatic cancer (PC) is one of the top five causes of death from cancer, and colorectal cancer accounts for 8.5% of cancer-related deaths [3][4][5]. Therefore, it is of great importance to pay attention to these diseases.
In clinical practice, many imaging techniques such as X-ray, computed tomography (CT), magnetic resonance imaging (MRI), and ultrasound have played a vital role in the detection and treatment of diseases [6]. Ultrasound, a noninvasive and real-time diagnostic technique, is the most commonly used method for detecting and diagnosing human digestive diseases [7]. However, the interpretation and analysis of ultrasound images depend deeply on the subjective judgment and experience of human experts. Radiologists may make mistakes due to exhaustion when dealing with a large number of images [8].
Artificial intelligence (AI) is defined as computer algorithms created by humans and improved with analogs of the thoughts, judgments, and reactions that take place in the human brain. In recent years, radiologists have increasingly embraced the aid of AI-powered diagnoses. AI can make a quantitative analysis by recognizing the information of images automatically and is widely applied in the medical images of ultrasound in diffuse liver diseases, focal liver lesions, PC, and colorectal cancer. In this review, we described the development of AI-based ultrasound in the aforementioned applications. In addition, we also discussed the future opportunities and challenges of AI-based ultrasound.

Deep learning
Deep learning is at the leading edge of AI and is developing rapidly. Deep learning is described as a group of artificial neural network (ANN) algorithms, which include many hidden layers. Namely, deep learning depends on a subset of algorithms that try to model high-level abstractions [12].
Recently, convolutional neural networks (CNNs) are the preferred type of deep learning architecture in the assessment of medical images [13]. CNNs consist of an input layer, multiple hidden layers, and an output layer (Figure 1). The hidden layers include convolutional layers, pooling layers, connected layers, and normalization layers. Convolutional layers and pooling layers can complete feature extraction and aggregation [9].

Diffuse liver diseases
Diffuse liver diseases display a failure in the metabolic and synthesis processes of the liver [14]. Liver biopsy is the gold standard for the diagnosis of fibrosis and NAFLD. However, liver biopsy is an invasive process that has many complications such as hemorrhage, biliary peritonitis, and pneumothorax [15]. In addition, liver biopsy is not feasible for the long-term management of patients with chronic liver diseases. Noninvasive liver imaging methods such as CT, MRI, and ultrasound have been extensively studied. Ultrasound is one of most common methods to diagnose liver diseases due to its noninvasiveness, inexpensive price, and real-time ability. Machine learning algorithms based on ultrasound have been applied for analysis of steatosis and the staging of liver fibrosis. Table 1 shows the application of ultrasound-based AI in diffuse liver disease.

Fatty liver diseases:
An excess amount of fat in the liver cells is found in fatty liver diseases (FLD). The main causes of FLD include obesity, alcoholism, diabetes, nonalcoholic steatohepatitis, drugs, and toxins [16,17]. FLD is related to the growing risk of cirrhosis and liver cancer. The most common cause of FLD is NAFLD, which ranges in prevalence from 25% to 45% [18]. Several noninvasive imaging methods such as CT, MRI, and ultrasound can diagnose NAFLD [19]. Ultrasound is the cheapest diagnostic method with 93% sensitivity, while hepatic steatosis is greater than 33%[18].
Conventional ultrasound is commonly used for NAFLD evaluation, but its qualitative nature, doctor dependency, and unsatisfactory accuracy limits the application. Moreover, the ultrasound images of fatty liver and early cirrhosis have many common features, making it hard to distinguish the two diseases by the human eye [20].
In recent years, ultrasound-based AI has demonstrated high accuracy for detection of steatosis and represents excellent reproducibility and reliability.
Byra et al [21] created a CNN model to acquire features from B-mode ultrasound image. It was reported that they could assess the amount of steatosis present in the liver with the area under the receiver operating characteristic curve (AUC) of 0.98, and their approach may assist the doctors in automatically assessing the amount of fat in the liver clinically [21].
Biswas et al [22] revealed that a deep learning-based algorithm reached a superior performance for FLD identification and risk stratification with 100% accuracy and AUC of 1.0 when compared with a conventional machine learning system support vector machine (SVM) (accuracy: 82%, AUC: 0.79) and extreme learning machine (accuracy: 92%, AUC: 0.92).
Deep learning has also been applied to quantitatively evaluate NAFLD. The radiofrequency data of ultrasound displays much more information of hepatic microstructure than that of gray-scale B-mode images [23]. Han et al [24] developed a deep learning algorithm that used radiofrequency data for NAFLD assessment. The results revealed that the sensitivity, specificity, and positive predictive value (PPV) for NAFLD diagnosis were 97%, 94%, and 97%, respectively. They confirmed that the quantitative analysis of raw radiofrequency ultrasound signals showed the potential of identifying NAFLD and quantifying hepatic fat fraction [24].

Liver fibrosis and cirrhosis:
Patients with chronic liver disease may have no clinical symptoms for an extended period, or it may develop to fibrosis and cirrhosis [25]. The activation of the resting hepatic stellate cell into an activated myofibroblast plays an important role in the progression of liver fibrosis. The activated myofibroblast expresses abundant a-smooth muscle actin and collagen [26].
Cirrhosis, which consists of various nodules and is harder than the normal liver, is the advanced period of fibrosis [27]. Liver fibrosis and early cirrhosis are confirmed to be partly reversible. Therefore, the precise diagnosis of liver fibrosis is vital for the treatment and management of chronic liver disease patients.
In clinical practice, liver biopsy is the gold standard for the diagnosis of liver fibrosis. Various noninvasive modalities such as ultrasound and elastography have been used as alternatives to liver biopsy. Some studies suggest that AI models based on ultrasound and elastography have great potential for the classification of liver fibrosis.   AI based on conventional ultrasound: AI based on conventional ultrasound has been applied to improve their performance for the diagnosis and grading of liver fibrosis.
Yeh et al [28] built an SVM model to analyze liver fibrosis. B-mode images of 20 fresh postsurgical human livers were used to assess ultrasound capacity in evaluating the stage of fibrosis. The study indicated the best classification accuracy of two, three, four, and six classes were 91%, 85%, 81%, and 72%, respectively [28]. The results confirmed that the SVM model may be suggested to assess diverse liver fibrosis stage.
Other than the B-mode ultrasound, duplex ultrasound has also been applied to diagnose liver fibrosis. Using an ANN model based on duplex ultrasound, Zhang et al [29] demonstrated that their model reached the accuracy, sensitivity, and specificity were 88.3%, 95.0%, and 85.0%, respectively. The ANN model included five ultrasonographic parameters: thickness of spleen, liver vein waveform, the hepatic parenchyma, liver artery pulsatile index, and hepatic damping index. The study suggested that their ANN model has the potential to diagnose liver fibrosis noninvasively [29].
Studies confirmed that radiomics show great performance in the grading of liver fibrosis. By the use of texture analysis to analyze ultrasound liver images, the study found the accuracies of S0-S4 were 100%, 90%, 70%, 90%, and 100%, respectively [30].
It was reported that deep learning has great potential for liver fibrosis evaluation. Lee et al [31] built a deep CNN and trained a four-class model (F0 vs F1 vs F23 vs F4) to predict METAVIR scores. They used 13608 ultrasound images of 3446 patients who accepted surgery, liver biopsy, or transient elastography to train the deep CNN model. The model achieved a higher AUC of 0.857 for the classification of cirrhosis compared with five radiologists (AUC range, 0.656-0.816; P < 0.05) using the external test set [31].
AI based on ultrasound elastography: ultrasound elastography has been performed to acquire quantitative assessment of liver tissue stiffness, which is related to the grades of fibrosis. These technologies include strain elastography and shear wave elastography (SWE) [32]. Recently, some studies confirmed that the AI based on SWE has great value to identify and stage liver fibrosis.
Compared to conventional radiomics, a multiparametric ultrasonic model using machine learning algorithms demonstrated better manifestation in fibrosis assessment [33]. By quantifying color information from SWE images, Gatos et al [34,35] created an SVM model that could differentiate patients with liver diseases from controls with accuracy, sensitivity, and specificity of 87.3%, 93.5% and 81.2%, respectively.
Deep learning has also been applied in the assessment of liver fibrosis. A multicenter study used deep learning radiomics on 2D-SWE ultrasound images for the classification of liver fibrosis[36]. 2D-SWE ultrasound images had higher AUCs of 0.97 for F4, 0.98 for ≥ F3, and 0.85 for ≥ F2 fibrosis when compared with standard 2D-SWE.
It is necessary to contain a large training dataset for deep learning. However, it is difficult and expensive to get abundant medical images in clinics. One method to solve this problem is the employment of transfer learning (TL), which can enhance the performance by TL from other areas to the ultrasound area [37]. A study developed a CNN model by TL radiomics to assess ultrasound images of gray-scale modality and elastogram modality for the grade of accurate liver fibrosis. TL in gray-scale modality and elastogram modality revealed much higher diagnostic accuracy of AUCs compared with non-TL. Multimodal gray-scale modality + elastogram modality was confirmed to be the most precise diagnostic model with AUCs of 0.930, 0.932, and 0.950 for classifying ≥ S2, ≥ S3, and S4, respectively. It

Focal liver lesion
Focal liver lesions (FLLs) are described as an abnormal part of the liver mainly coming from hepatocytes, biliary epithelium, and mesenchymal tissue [39]. Due to its cheap price, noninvasiveness, and real-time imaging, ultrasound is the preferred method for the diagnosis of FLLs. Based on this trend, the AI models using ultrasound images have more advantages over CT and MRI in routine clinical applications [40]. Table 2 shows the application of ultrasound-based AI in FLLs. In addition to TIC, extracting features except TICs from a region of interest on CEUS images and videos was also applied in AI. A two-stage multiview learning framework, which was the integration of deep canonical correlation analysis and multiple kernel learning for CEUS-based computer-aided diagnosis, was proposed to identify liver tumors. The deep canonical correlation analysis-multiple kernel learning framework achieved performance for discriminating benign from malignant liver tumors with the accuracy, sensitivity, and specificity of 90.4%, 93 Căleanu et al [53] reported the 5-class classification of liver tumors using deep neural networks with an accuracy of 88%. In this study, deep neural network algorithms were compared with state-of-the-art architectures, and a novel leave-one-patient-out evaluation procedure was presented.

The application of AI in the diagnosis of benign and malignant
All these studies indicated that AI based on conventional ultrasound and CEUS played a vital role in the detection and distinction of FLLs.  accurate assessment of great numbers of image features from medical images. These features that are difficult to detect by the human eye can be detected by machine learning or deep learning. AI models based on radiomics has also been reported to be applicable for the management of HCC, such as the prediction of microvascular invasion (MVI), curative transarterial chemoembolization (TACE) effect, recurrence after thermal ablation, and prognosis. Predicting MVI: MVI is described as the invasion of tumor cells within a vascular space lined by endothelium. It has been proven that MVI is a predictor of early recurrence of HCC and poor survival outcomes [54]. The only way to confirm MVI is via histopathology after surgery. Patients with HCC can receive a great benefit when MVI is identified noninvasively and accurately before surgery [55]. The application of AI based on gray-scale ultrasound images and CEUS indicated good performance in predicting preoperative MVI.
A study indicated that the radiological features of gray-scale ultrasound images of gross tumoral area predicted preoperative MVI of HCC with an AUC of 0.81 [56]. A CEUS-based radiomics score was built for preoperative prediction of MVI in HCC[57]. The radiomics nomogram revealed great potential in the detection of MVI with an AUC of 0.731 compared with the clinical nomogram with an AUC of 0.634. It was indicated that the radiomics data based on ultrasound was a single predictor of MVI in HCC. Our group created a radiomics model based on CEUS to evaluate MVI of HCC patients before surgery. The model revealed a better detection in the primary group with an AUC of 0.849 vs 0.690 as well as the validation group with an AUC of 0.788 vs 0.661 when compared with the clinical model. We confirmed that the portal venous phase, delay phase, tumor size, rad-score, and alpha-fetoprotein level were single predictors related to MVI [58].
Predicting curative TACE effect: Pathways participating in important cancer-related progression, such as cell proliferation and angiogenesis, are major goals for the treatment of HCC patients. Additionally, transcription factors and cell cycle regulators are also considered to be interesting for anti-HCC drugs [ Predicting recurrence after thermal ablation: Thermal ablation has been confirmed to be an available therapy for early-stage HCC patients who are unsuitable for operation or recurrence after surgery [64]. In addition, the recent 2-year recurrence rates of HCC patients who underwent thermal ablation were reported as 2%-18% [65]. The accurate preoperative prediction of thermal ablation outcomes is of great importance for HCC patients. Compared with other imaging modalities, CEUS is radiation-free and has better temporal resolution when revealing the blood supply of the tumor. The application of AI based on CEUS could be performed for the preoperative prediction of thermal ablation outcomes.
A radiomics model was created to predict the early and late recurrence of HCC patients who accepted thermal ablation [66]. The combined model including CEUS, ultrasound radiomics, and clinical factors showed better performance for early recurrence with an AUC of 0.89 and for late recurrence prediction with a C-index of 0.77.
Predicting the prognoses: Surgical resection (SR) and radiofrequency ablation (RFA) are common curative strategies for HCC patients diagnosed at the early stage [64]. Some studies have compared the long-term survival of RFA and SR for early-stage HCC patients [67,68]. However, the conclusions were sharply different. Hence, it is necessary to find useful predictive means to select the optimal patients who are suitable for RFA or SR before surgery. AI models based on CEUS had great performance for the prediction of progression-free survival (PFS).
A deep learning-based radiomics from CEUS images was built to predict the PFS of SR and RFA for HCC patients. Both SR and RFA models achieved high prediction accuracy of 2-year PFS. They also identified that a higher average probability of 2-year PFS may be acquired while some RFA and SR patients exchange their choices [69]. By utilizing conventional ultrasound images and CEUS, these AI prediction models can be applied in the individualized management of HCC patients.

Gastric mesenchymal tumors
The majority of gastric mesenchymal tumors are occasionally found during routine esophagogastroduodenoscopy examinations. The incidence of gastric mesenchymal tumors is uncertain, but the prevalence of subepithelial tumors identified under endoscopy in Korea was reported as 1.7% [70]. Most gastric mesenchymal tumors are gastrointestinal stromal tumors (GISTs), which may metastasize to the liver and peritoneum after surgery [71,72]. Hence, distinguishing GISTs from benign mesenchymal tumors such as leiomyomas or schwannomas is of great importance in clinic practice. Endoscopic ultrasonography (EUS) is a common method to assess gastric mesenchymal tumors. It helps doctors evaluate the detailed size, shape, origin, and border of the lesions [73][74][75]. But the interpretation of EUS images by endoscopists is subjective and has poor interobserver agreement. Recently, EUS image interpretation using AI has developed rapidly and is applied to distinguish GISTs from benign mesenchymal tumors.
A convolutional neural network computer-aided diagnosis (CNN-CAD) model based on EUS images was developed to assess gastric mesenchymal tumors. They reported the model distinguished GISTs from non-GIST tumors with 83.0% sensitivity, 75.5% specificity, and 79.2% accuracy [76]. The CNN-CAD model had the potential to provide diagnostic assistance to endoscopists in the future. October 14, 2022 Volume 28 Issue 38

Pancreatic diseases
EUS is currently a common tool to diagnose pancreatic diseases in clinical practice. However, the specificity for the diagnosis of pancreatic diseases using EUS images is low and deeply depends on the subjective judgment of endoscopists. Studies have confirmed that AI based on EUS improves their performance for the diagnosis of pancreatic diseases. Recently, AI using EUS images has been applied in the differential diagnosis of PC, distinguishing intraductal papillary mucinous neoplasms (IPMNs) and detecting pancreatic segmentation.
Pancreatic cancer: PC is relatively uncommon, with an incidence of 8-12 per 100000 per year. PC is attributed to hereditary germline or somatic acquired mutations in some genes such as tumor suppressor genes and cell cycle genes. These mutations are also associated with the progression and metastasis of PC. Moreover, shortened telomerase, cell turnover, and genomic instability have an important role in the development of PC [77]. The early diagnosis and surgery of PC, especially for lesions less than 1 cm, can achieve long-term prognoses with a 5-year survival rate of 80.4% [78]. However, PC is most frequently detected at an advanced stage, and the 5-year survival rate remains as low as 3%-15% [79]. Hence, early detection is vital for the treatment of PC patients. Studies have reported that AI based on EUS has great performance for the diagnosis of PC.
AI based on B-mode EUS: AI models based on B-mode EUS have been applied to improve their performance for the diagnosis of PC. Norton et al [80] first reported the use of CAD utilizing EUS images in pancreatic diseases in 2001. The study included 14 patients with focal chronic pancreatitis and 21 patients with PC. They showed the diagnostic sensitivity of the two diseases was 89%, and the overall accuracy was 80% [80]. However, this study cannot be referred to as AI-CAD in current applications as the number of patients was limited and the resolution of images were very low.
With the development of AI, ANN and SVM presented good performance in the diagnosis of PC [81][82][83]. Das et al [81] developed an ANN model to distinguish chronic pancreatitis from PC. The results achieved 93% sensitivity, 92% specificity, 87% PPV, 96% negative predictive value (NPV), and 0.93 AUC [81]. By using a multilayered neural network, the study confirmed the first machine learning results for the EUS images of the pancreas. But the sample size was small and lacked pathological evidence in the chronic pancreatitis and normal pancreas groups.
By selecting better texture features that included multifractal dimensional features, a quantitative measure of fractality (self-similarity), and complexity from EUS images, a SVM prediction model was created to identify PC and non-PC patients [83]. The model reached 97.98% accuracy, 94.32% sensitivity, 99.45% specificity, 98.65% PPV, and 97.77% NPV. The study demonstrated that SVM using EUS images is a useful tool for diagnosing PC and pancreatic diseases.
It was reported that AI was also applied for the age-dependent pancreatic changes on EUS images of PC cases. Ozkan et al [84] suggested a high-performance CAD model applying ANN to discriminate PC and noncancer patients in three age groups. In the under 40-year-old group, the accuracy, sensitivity and specificity were 92.0%, 87.5%, and 94.1%, respectively. In the 40-year-old to 60-year-old group, the accuracy, sensitivity, and specificity were 88.5%, 85.7%, and 91.7%, respectively. In the > 60-year-old group, the accuracy, sensitivity, and specificity were 91.7%, 93.3%, and 88.9%, respectively. The total performance of this model showed the accuracy, sensitivity, and specificity were 87.5%, 83.3%, and 93.3%, respectively.
Besides machine learning, deep learning has been applied to B-mode EUS images for analysis of PC. A CNN model using EUS images was developed for the detection of PC [85]. The sensitivity, specificity, PPV, and NPV were 90.2%, 74.9%, 80.1%, and 88.7%, respectively. The CNN model included six normalization layers, seven convolution layers, four max-pooling layers, and six activation layers. The EUS-CNN application was first reported to have the potential to detect PC from EUS images.
AI based on EUS elastography: Real-time EUS elastography can provide more information about the features of pancreatic masses by the use of strain assessment. It was reported that EUS elastography has been applied in the differential diagnosis of pancreatic lesions. However, the accuracy and reproducibility were unstable [86,87].
The application of AI improves their performance in the diagnosis of PC. A prospective, blinded, multicentric study using EUS elastography by ANN was performed in focal pancreatic lesions [88]. They demonstrated the sensitivity, specificity, PPV, and NPV values for the diagnosis of PC were 87.59%, 82.94%, 96.25%, and 57.22%, respectively. The study suggested that the ANN model may provide fast and accurate diagnoses in the clinical.
AI based on contrast-enhanced EUS: Contrast-enhanced EUS has been used to enhance the detection of pancreatic lesions [89]. AI based on contrast-enhanced EUS has great performance for the diagnosis of PC. An ANN model based on the TIC analysis from contrast-enhanced EUS images was designed to diagnose PC and chronic pancreatitis. The study reached 94.64% sensitivity, 94.44% specificity, 97.24% PPV, and 89.47% NPV [90]. The study suggested that the model could provide additional diagnostic value to CEUS interpretation and EUS fine needle aspiration results.

IPMNs:
IPMNs are considered to be precursor lesions of pancreatic adenocarcinoma. Early surgical resection of IPMNs can provide a survival benefit for patients [91]. EUS is often used to assess the October 14, 2022 Volume 28 Issue 38 malignancy of IPMNs in clinics. Several predictive techniques were used to diagnose the malignancy of IPMNs with no satisfactory results (70%-80%) [92,93]. Compared with human diagnosis and conventional EUS features, AI via deep learning algorithms was confirmed to be a more exact and objective way for the differential diagnosis of malignant IPMNs. Kuwahara et al [94] performed a predictive CNN model using EUS images to detect malignant IPMNs. The model reached 95.7% sensitivity, 94.0% accuracy, and 92.6% specificity. The accuracy was higher compared with the diagnosis of a radiologist (56.0%). The author suggested that the application of AI can evaluate malignant IPMNs before surgery.
Pancreatic segmentation: AI using EUS images has also been applied in pancreatic segmentation. A deep learning-based classification system was created to utilize the "station approach" in EUS of pancreas [95]. The system obtained 90.0% accuracy in classification and 0.770 and 0.813 in blood vessel and pancreas segmentation, respectively. The results were similar to that of EUS experts. Thus, this study revealed that AI has the feasibility to detect the station and segmentation of the pancreas.

Colorectal tumors
Colorectal cancer is the third most common cancer worldwide and accounts for the second leading cause of cancer-related deaths. Moreover, a growing number of patients diagnosed with rectal cancer are under 50-years-old [96]. Colorectal cancer is attributed to gene mutations of epithelial cells, such as oncogenes, tumor suppressor genes, and DNA repair genes. The specific molecular mechanisms implicated in this type of cancer may include the instability of chromosomes and microsatellites [97].
Recently, some researchers studied tumor deposits (TDs) of rectal cancer. TDs are described as focal aggregates of adenocarcinoma located in the surrounding fat of the colon or rectum. They are discontinuous with the primary tumor and unrelated to a lymph node [98,99].
It was reported that a patient who is TD-positive has more malignant tumors, with decreased diseasefree survival and overall survival [100]. However, TDs are often diagnosed by pathology only after surgery. Hence, the noninvasive preoperative prediction of TDs is important for rectal cancer patients. EUS is currently a common tool to detect rectal masses. Recently, ultrasound-based radiomics have been applied to predict the status of TDs.
Chen et al [101] developed an ANN system using ultrasound radiomics and clinical factors to predict TDs. Endorectal ultrasound and SWE examinations were conducted for 127 patients with rectal cancer. The accuracy was 75.0% in the validation group. The model reached 72.7% sensitivity, 75.9% specificity, and 0.743 AUC. The study suggested that ultrasound-based radiomics has the potential for the prediction of TDs before treatment. Table 3 shows the application of ultrasound-based AI in gastrointestinal disease.

CONCLUSION
In recent years, AI models using ultrasound images have developed rapidly. They can offer a more precise and efficient diagnosis and ease the burden of doctors. AI based on ultrasound has been confirmed to be helpful in diffuse liver diseases and FLLs, such as assessing the severity of NAFLD and the grade of liver fibrosis, distinguishing benign and malignant liver lesions, predicting the MVI of HCC, curative TACE effect, and prognoses after thermal ablation. In addition, AI based on EUS has great performance in gastrointestinal diseases, such as distinguishing gastric mesenchymal tumors, differential diagnosis of PC, distinguishing IPMNs, and predicting the status of TDs in rectal cancer.
However, the application of AI based on ultrasound in clinical practice has some limitations. The main reason may be due to the high variability between radiologists in ultrasound image acquisition and interpretation [102]. Hence, it is necessary to unify the ultrasonic image acquisition process as well as the standard of ultrasonic data measurement during the ultrasound examination.
In addition, some studies of AI-powered ultrasound were retrospective and trained on limited data offered by a single hospital with potential data selection bias, and the amount of data in the training set was not enough. Abundant multicenter prospective studies should assure the efficiency and stability of these AI models. Additionally, deep learning needs a large number of images, so it is necessary to establish an abundant database with common collaborative efforts.
In addition, the application of AI based on EUS has some limitations. The number of EUS examinations is overwhelmingly low compared to other examinations such as endoscopy and CT, especially in gastrointestinal diseases.
In the future, AI based on ultrasound may be used to develop highly accurate and more efficient models for more digestive diseases such as peptic ulcers, stomach neoplasms, inflammatory bowel disease, and so on. These models may heavily reduce the workload for doctors by automatic identi- fication of disease on radiologic and histopathologic images. Moreover, the application of AI can enable building individual management for patients as well as predicting disease progression and complications in clinics. Additionally, AI may improve distance teaching by remote monitoring and enhance medical services in undeveloped areas.