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Prevalence of metallo‑beta‑lactamase producing Acinetobacter baumannii isolated from intensive care unit in tertiary care hospitals.

INTRODUCTION
The emergence of Metallo-beta-lactamase (MBL)-producing Acinetobacter baumannii has become a global concern in nosocomial infections. The aim of this study is to determine the prevalence of MBL producing genes among clinical isolates of A. baumannii from hospitalized patients.


METHODS
This study was performed from October 2015 to October 2016 at three teaching hospitals located in Isfahan, Iran. Totally, 100 A-baumannii isolates were collected from clinical specimens and identified as A-baumannii using standard microbiological methods. Antimicrobial susceptibility test was determined by disc diffusion method according to the CLSI. Furthermore, the determination of bla IMP-1, bla IMP-2, bla VIM-1, bla VIM-2and bla SIM-1 was detected by PCR.


RESULTS
Totally, Sixty-eight percent (68%) of isolates of A. baumannii were recovered from tracheal aspirate. According to the antibiotic susceptibility pattern, the highest level of resistance was against ciprofloxacin (99%), while among tested antibiotics amikacin (10%) was found to be the most effective. 21%, 4%, 7% and 6% isolates carried bla IMP-1, bla IMP-2, bla VIM-1 and bla VIM-2 genes, respectively. Also, bla SIM-1 was not detected in any of the isolates.


CONCLUSION
The results of this study showed high rate of the MBL producing A-baumannii isolates in our region and displayed that MBLs producing A-baumannii strains are emerging threats to ICUs.