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Studies on the cross-reaction between human growth hormone and human chorionic somatomammotropin in radioimmunoassay systems.

ABSTRACT The effects of unlabeled human growth hormone (HGH) and human chorionic somatomammotropin (HCS) on the binding of 131I-HGH or 131I-HCS by anti-HGH or anti-HCS sera have been investigated. The reactions between them discriminated clearly between the homologous and cross-reacting unlabeled hormones. In contrast, the reactions between 131I-HGH and anti-HCS sera or 131I-HCS and anti-HGH sera were inhibited to a similar extent by either unlabeled hormone. The results obtained with combinations of unlabeled HGH and HCS indicate that values for HGH, as determined by radioimmunoassay, are likely to be overestimated in serum from pregnant subjects but suggest that they may be corrected by use of a family of standard curves.