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MF-EA-705alpha & MF-EA-705beta, new metabolites from microbial fermentation of a Streptomyces sp.

As part of an antifungal discovery program, the crude extract of a Streptomyces sp. from Ecuador, MF-EA-705, was found to display broad-spectrum antifungal activity against Candida spp. and Aspergillus spp. The extract was found to contain the known weakly-antibacterial compound actinopyrone A (1), 1} previously isolated from Streptomyces pactum. We report herein the production, isolation, structure elucidation and biological activities of two new compounds MF-EA-705a (2) and MF-EA-705£ (3). A structurally similar compound has been reported in the microbial natural product literature, NFAT1 33 (4),2) known to be an immunosuppressiveagent in vitro. An 8-cm sporulation agar plate was inoculated with a 100 /il portion of frozen MF-EA-705 stock, and incubated at 28°C until growth (medium, beige-white) was observed (16 days). Sterile phosphate-buffered saline (6 ml) was then added. The MF-EA-705 mycelia and spores were gently scraped with a sterile loop, and a 3-ml portion of the resultant suspension was pipetted into a 500-ml flask containing sterile ATCC-172 media (150ml, 2% soluble starch, 1% dextrose, 0.5% NZ amine type A, 0.3% Difco beef extract, 0.5% Difco bacto peptone, 0.5% yeast extract, 0.1% CaCO3, presterile pH 7.0). The seed culture was incubated for 24hours at 28°C, 200rpm, and 85%