{'fraction_non_alphanumeric': 0.03274985883681536, 'fraction_numerical': 0.009599096555618294, 'mean_word_length': 6.414225941422594, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Blood mononuclear cells induce regulatory NK T thymocytes in anterior chamber‐associated immune deviation

Injection of antigen into the anterior chamber (AC) of the eye, animmunologically privileged site, is associated with the inductionof immune deviation, as evidenced by T helper cell (Th) 1 to Th2 cellpolarization. We recently demonstrated that AC‐associated immunedeviation (ACAID) is a thymus‐dependent phenomenon initiated by the formation of regulatory α,β T‐cell receptor‐positiveCD4− CD8− thymocytes(THYregs). In this study, the afferent and efferent limbsof this immunoregulatory loop were traced from peripheral blood to thethymus and then to the spleen by adoptive‐transfer assays. The resultsdemonstrate that (1) F4/80+ CD1+ peripheralblood mononuclear cells from mice whose ACs were injected withtrinitrophenol‐bovine serum albumin induce the appearance ofnatural killer (NK) 1.1+ THYreg innaïve recipients within 24 h of intravenous infusion; (2)these NK THYregs induce (or generate) suppressor‐effector Tcells in the spleens of adoptive recipients; (3) thesesuppressor‐effector spleen cells, but not the NK THYregsthemselves, directly inhibit the expression of delayed‐typehypersensitivity in sensitized recipients; and (4) peripheral bloodmononuclear cells from AC‐injected mice do not induce ACAID inthymectomized recipients. These results confirm our hypothesis thatACAID is a model of centrally induced dominant tolerance mediated byCD‐1‐dependent NK T cells of recent thymic origin. The results alsoprovide evidence of a novel tolerance induction pathway by whichblood‐borne antigen‐presenting cells generated by antigen injectioninto an immunologically privileged site transport antigen to the thymusand induce the formation and export of THYreg.