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Comparative study of the glial fibrillary acidic protein in vertebrates by PAP immunohistochemistry

Glial fibrillary acidic protein (GFA) has been visualized by direct peroxi‐dase antiperoxidase (PAP) immunohistochemistry in various vertebrates (cyclostomes, teleosts, amphibians, reptiles, birds, and several placental mammals). In this study GFA‐immunoreactivity (GFA‐I) was observed in all species examined except in cyclostomes and amphibians. Two types of immunoreactive elements were observed: astrocytes and long processes without visible somata.