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Optimization of Separation Conditions of Liquiritin in Preparative Liquid Chromatography

The aim of this work was to study the separation conditions of Liquiritin (LQ) in preparative liquid chromatography. Based on the inverse method, the adsorption isotherm parameters of LQ and the closely impurities were determined. Then the chromatographic conditions were optimized by combination of orthogonal design experiments and numerical simulation. The optimum injection concentration, injection purity of LQ and injection volume was found as 50 g/L, 45 % and 5 mL, respectively. The parameters of simulated moving bed chromatography were selected and the separation process was analysis. This method can give reference for purifying of low content component from the complex herbs.