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Establishment in continuous culture of a T‐lymphoid cell line (HD‐Mar) from a patient with Hodgkin's lymphoma

A new cell line, HD‐Mar, was established from a pleural effusion of a patient with Hodgkin's lymphoma. Formation of E rosettes, sensitivity to anti‐T serum, elevated terminal deoxynucleotidyl transferase activity, presence of T‐cell and the common ALL membrane antigens, morphology, and cytochemical staining indicate that the HD‐Mar line is of thymic derivation. Absence of any immunoglobulin determinants, the lack of EBNA or any other EBV‐associated antigen or function are also characteristics associated with established T‐ cell‐derived lymphoma cell lines. Karyotype analysis indicated a tetraploid origin of the cell line.