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Time-resolved Detection of Singlet Oxygen in a Transmission Microscope¶

The time-resolved absorption spectrum of singlet oxygen [O2(aDg) → O2(bSg)] has been recorded in the region ;5100–5300 cm21 from air-saturated polystyrene samples using a microscope attached to a step-scan Fourier transform IR spectrometer. Singlet oxygen signals were observed with a time resolution of ;160 ns from sample volumes of ;20 nL using moderate data-acquisition times. These data indicate that it is reasonable and worthwhile to consider the further development of a transmission microscope as a viable tool to create singlet oxygen images of inhomogeneous samples including samples of biological importance.