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Measurement of microfluidic channel by using multiple-scans overlap mode of optical coherence tomography

A method for measuring the channel size of microfluidic chip by using optical coherence tomography (OCT) is investigated. Based on spectral-domain OCT imaging and image processing, an OCT system for measurement of the microfluidic channel size is developed, which is suitable for planar two-dimensional measurement. The cross-sectional images of the micro-channel of a microfluidic chip are obtained by using OCT continuous B-scan technology. With image processing for the acquired image in noise elimination and dispersion compensation, the size of the micro-channels in depth is achieved. Comparing with the single-scan OCT imaging mode, back-passing multiple-scans mode has the capability of high image quality to achieve high measurement precision of micro-channel size.