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Chromatographic differences between circulating and pituitary thyrotropins.

ABSTRACT To explore the possibility that circulating immunoreactive human thyrotropin (hTSH) may be heterogeneous, sera were obtained from 3 untreated patients with primary hypothyroidism before and after the administration of synthetic thyrotropin-releasing hormone (TRH), and were examined by both gel chromatography on Sephadex G-100 and anion exchange chromatography on DEAE-Sephadex A-50. For comparison, purified unlabeled pituitary hTSH was examined under identical conditions. 125I-pituitary hTSH was cochromatographed in all experiments as an internal standard. Unlabeled pituitary hTSH and serum hTSH were measured in all chromatographic fractions by radioimmunoassay. Unlabeled pituitary hTSH eluted as an essentially symmetrical single component during gel chromatography but appeared to contain multiple components during anion exchange chromatography. In comparison to a series of reference proteins of known molecular size, the molecular radii of 125I-pituitary hTSH and unlabeled pituitary hTSH were esti...