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Ultravibratomy Applied in Ultrathin Sectioning Results in Sections Without Distortions and Compression.

When resins are ultrathin sectioned they are compressed in the sectioning direction. The amount of compression depends very much on the sectioning angle on one hand, and on the properties of the resin on the other hand. Shortening of the sections in the sectioning direction is in the range of 5 to 35%. Even more compression occurs during cryosectioning of vitreous samples at very low temperatures (-170°C); the range was measured to be 30 to 60% (1). To reduce these compression phenomena, there is basically only one approach possible. The sectioning angle has to be reduced as much as possible. In the past it was shown that by reducing the knife angle from 45° to 35°, a reduction of compression by appoximately one third was achieved (2). The problem with diamond knives is, that the smaller the knife angle, the more susceptible the cutting edge becomes. In practice angles smaller than 35° are not used because of this limitation.