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189 THE EFFECT OF LIFESTYLE-RELATED DISEASES ON THE PRODUCTION OF CYTOKINES FROM SKELETAL MUSCLE (MYOKINES)

Background: Myokines, which are cytokines secreted from skeletal muscle after exercise, exert a beneficial metabolic effect in an auto or paracrine fashion. However, it is unclear how lifestyle-related diseases such as hypertension or diabetes impact the production of myokines. We examined the effect of lifestyle-related diseases on the modulation of myokine production using Spontaneously Hypertensive Rats (SHR). Methods: 20-week-old Male SHR rats were divided into two groups, SHR fed a normal diet (SHR-ND) and SHR fed a high fat & fructose diet (SHR-HF) for 12 weeks. At 32 weeks of age, soleus muscle (SOL) and extensor digitorum longus muscle (EDL) were extracted. The molecular biological analyses including the expression of myokines in the excised muscle were performed. Wistar Kyoto rats (WKY) were used as controls. Results: There was no difference in the weight of SOL among three groups, however the weight of EDL was lower in SHR-ND and SHR-HF than in WKY. There was no difference in the expression of IL-6 in SOL among three groups, however that in EDL after exercise was higher in SHR-ND and SHR-HF than in WKY. The expression of IL-15 was low in SOL, and that in EDL after exercise was highest in WKY, followed by SHR-ND, SHR-HF. The expressions of ubiquitin–ligases, atrogin1 and MuRF1 were higher in SHR-HF than in SHR-ND and WKY. Conclusions: These findings may indicate that myokines are more highly expressed in EDL than in SOL, and these productions are impaired by lifestyle-related diseases and mediate the muscle metabolism.