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CRUCIAL ROLE OF TDZ IN THE QUICK REGENERATION OF MULTIPLE SHOOTS OF CLITORIA TERNATEA L Research Article

An efficient and rapid micropropagation technique was developed for rare medicinal plant Clitoria ternatea. The crucial role of TDZ was investigated on in vitro shoot proliferation from nodal explants of Clitoria ternatea. Murashige and Skoog (MS) medium containing TDZ(0.05- 2.5µM) was evaluated to develop multiple shoot buds and maintaining high rates of shoot multiplication on hormone free MS medium. The maximum number of shoots (15-20) with (80%) response was obtained on MS medium supplemented with (2.50µM) TDZ. In vitro developed shoots were rooted on half strength MS medium supplemented with IBA (2.0µM). Complete plantlets were then hardened, acclimatized and finally transferred to natural conditions where they exhibited 85% survivability.