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Extraction of Tannins from Wild Persimmon Leaf with Semi-Bionic and Antioxidation Analysis

Tannin was extracted from wild persimmon leaf using Semi-Bionic approach. The technology of extraction was optimized and the antioxdation activity was detected. The results showed that the Semi-Bionic approach improved the scavenging activity of tannins on radicals, including OH·, DPPH·, and ABTS+·. The half maximal inhibitory concentration values were lower than that of the water-bath heating and enzymic method. Experimental results of orthogonal optimization showed that the yield of tannin reached 206.8 mg/g, higher than that of the sample obtained with water-bath heating and enzymic method under the following conditions: 40 ℃, 60 min, 50% solvent mass fraction, three extraction times with p H values of 2.0,7.8,and 9.0, and raw material particle size of 120 mesh.