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Optimization of a Microarray Probe Design Focusing on the Minimization of Cross-hybridization

Microarrays are extensively used for highthroughput gene expression analyses in molecular biology. Microarray analysis is reliable if the probe binds specifically to the intended target transcript. Cross-hybridizations of microarray probes is one of the main systematic errors which is influenced by microarray probe design. Newly released genome annotations make it possible and necessary to improve given probe designs in order to reduce this source