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PERINEURAL IMPLANTATION OF STEM CELLS FOR CORRECTION OF PATHOLOGICAL PROCESSES IN HEART AND LIVER

Pathological processes in cardiovascular system and liver are fatal for citizens of all over the world. New preventive and therapeutic approaches are needed in this field of medicine. Using of cell technologies for the treatment of patients with heart attack and/or cirrhosis is the one. Systemic or intraorganic stem cells (SCs) administration has shown poor therapy results. New technology was substantiated in our experiments on 27 male Wistar rats weighing 240–270 g. Vagus was cut off in 9 animals after cervical midsection on the left side under ketamine‐xylazine‐acepromazine (55.6, 5.5, and 1.1 mg/kg, respectively) anesthesia (I) in order to initiate destructive processes in heart. 9 rats were subjected to laparotomy followed by 2×2 mm injury of liver right lobe (II). Both laparotomy and cervical midsection but without liver and vagus damage was performed in the last 9 rats. All the rats received 10 thousands SCs in 10 μl culture medium into perineural space of vagus on the right side of the neck during anesthesia. SCs were marked with monoclonal antibodies to CD90 and PKH67 Green Fluorescent Cell Linker. Two animals from each group were decapitated in one day, one week and two weeks and slices from heart and liver 8 μm in thickness were prepared. SCs distribution was visualized using Zeiss AxioVert 200M fluorescence microscope with Zeiss AxioCam HRm CCD camera. Fluorescent cells were revealed in heart and liver areas in groups I and II, respectively. Zero fluorescent cells have been found in heart and liver of group III. Therefore, perineural way of SCs administration is an alternate path of SCs delivery to damaged inner organs only.