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OPTIMIZATION OF MEDIA FOR ANTIMICROBIAL COMPOUNDS PRODUCTION BY BACILLUS SUBTILIS

Bacillus subtilis is one of the most important producers of diverse antimicrobial compounds. This bacterium grows and produces antibiotics on different substrates. The increase of the antibiotics yield can be achieved by changing the conditions of cultivation and the composition of the culture media. In this study, response surface methodology was used for optimization of glycerol, sodium nitrite, and phosphate content in media for production of antibiotics effective against Staphylococcus aureus. As biosynthesis strain Bacillus subtilis ATCC 6633 was used. The developed model predicts that the maximum inhibition zone radius (38.08 mm) against Staphylococcus aureus and minimal amount of residual nutrients (glycerol 1.75 g l −1 , nitrogen 0.21 g l −1 , phosphorus 0.18 g l −1 ) are achieved, when the initial content of glycerol, sodium nitrite, and phosphate are 49.99 g l −1 , 1.00 g l −1 , and 5.00 g l −1 , respectively.