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Molecular pathogenesis of Salmonella enterica serotype Typhimurium-induced inflammatory responses

Molecular Pathogenesis of Salmonella enterica Serotype Typhimurium-Induced Inflammatory Responses. (May 2007) Josely Ferreira Figueiredo, D.V.M., Universidade Federal de Uberlândia; M.Sc., Universidade Federal de Minas Gerais Chair of Advisory Committee: Dr. L. Garry Adams We demonstrated that infection of HeLa cells, which are non-responsive to flagellin, with wild type Salmonella enterica serotype Typhimurium (S. typhimurium) activated chemokine expression at higher level than S. typhimurium lacking sipAsopABDE2, indicating that the corresponding effector proteins (SipA, SopA, SopB, SopD and SopE2) are required to induce chemokines independent of flagellin. The S. typhimurium sipAsopABDE2 mutant complemented with sipA activated IL-8 expression at significantly higher level than a S. typhimurium sipAsopABDE2 mutant. However, extracellular addition of recombinant SipA failed to induce IL-8. Phosphorylation analyses demonstrated that S. typhimurium carrying a chromosomal copy of sipA (sopABDE2 mutant) induced phosphorylation of CREB1, JUN and p38MAPK, which are proteins involved in IL-8 expression. The contribution of effector proteins to S. typhimuriuminduced intracellular Ca mobilization and its role in IL-8 expression and bacterial