{'fraction_non_alphanumeric': 0.032014380304443785, 'fraction_numerical': 0.0210016155088853, 'mean_word_length': 4.888263665594855, 'pattern_counts': {'":': 0, '<': 4, '<?xml version=': 0, '>': 1, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Author Correction: The balance of metagenomic elements shapes the skin microbiome in acne and health

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

The skin is the largest organ in the human body and functions as the first line of defense by providing a protective barrier between the environment and inner body. The skin harbors several hundreds of resident microorganisms, which function in communities and protect the body from invasion of pathogens 1,2 . Several studies have shown that shifts in the skin microbiota are associated with various skin diseases [3][4][5][6][7] .
Acne vulgaris (commonly called acne) is the most common skin disease, affecting 80-85% of the population 8,9 . It is most prevalent in adolescents and rarely occurs in people over the age of 50 10,11 . Although acne is not life threatening, it can lead to severe pain and scarring on the skin, and has profoundly negative psychosocial effects 12,13 . Acne is a disease of the pilosebaceous unit (commonly known as the hair follicle) 14 . While its etiology is still unclear with multiple factors involved, the Gram-positive lipophilic anaerobe Propionibacterium acnes has been thought to play a role in acne pathogenesis 15,16 . On the other hand, P. acnes is believed to contribute to skin health, preventing the colonization of opportunistic pathogens via its ability to convert sebum to free fatty acids and to maintain an acidic skin pH 17,18 .
In our previous characterization of the skin microbiome in healthy individuals and acne patients using 16S ribosomal RNA (rRNA) analysis, we revealed that certain P. acnes strains were highly associated with acne while some strains were associated with healthy skin 5 . At the genome level, P. acnes strains from different lineages harbor various genetic elements associated with either disease or health 5,19,20 . As individuals often harbor multiple P. acnes strains simultaneously which may have different roles in disease or health, it is unclear how the colonization of multiple strains in the follicle may affect the overall virulence or health properties of the skin microbial community.
Microbiome analyses based on the 16S rRNA or other phylogenetic marker genes and elements are often limited in providing molecular insight of disease association. Metagenomic shotgun sequencing analysis reveals not only the taxonomic composition but also the functional potential of the microbiome, and thus can provide better understanding of the potential microbial involvement in health or disease at the molecular level. To date, Figure 1. Bacteria dominate the skin follicular microbiome. (A) A box-and-whiskers plot comparing the relative abundances of bacteria, fungi, and P. acnes phage in the follicular microbiota of acne patients (n = 38), age-matched healthy individuals (n = 30), healthy individuals over 55 (H55 + ; n = 4), and all healthy individuals combined (n = 34). (B) A few fungal organisms were found in the follicle. Sequencing reads pooled from all subjects mapped to six fungal species, with less than 1X coverage for any species. (C) The relative abundance of P. acnes phage in all the samples suggests an increased prevalence and abundance of P. acnes phage in healthy individuals and a trend of increased phage abundance with age. (D) The relative abundances of bacterial species in the follicle. Each column represents the relative abundances of the bacterial species found in each individual. P. acnes was the dominant skin bacterium in all but one individual. On average P. acnes accounted for 91% of the bacterial taxa identified. An increase in the average relative abundances of P. acnes and P. granulosum was observed in the healthy individuals, whereas an increase in the average relative abundances of minor taxa was observed in the acne group. Five major skin bacterial species (P. acnes, P. humerusii, P. avidum, P. granulosum, and S. epidermidis) are shown separately from the phyla that they belong to.
At the species level, P. acnes was the most prevalent and abundant. It was found in all 68 individuals with an average relative abundance of 91.0% ( Fig. 1D; Table 1). The healthy individuals had a slightly higher relative abundance of P. acnes than the acne patients (93.8% vs. 88.5%). We also compared the differences in P. acnes strain populations between acne patients and healthy individuals. The relative abundances of the dominant P. acnes strains were assigned based on the read coverages of the 16S rRNA SNPs that distinguish the ten most common (top ten) P. acnes ribotypes (RTs) 5 (Fig. S2). We found that acne patients had a higher diversity of P. acnes population (Shannon index = 1.07) with more RTs (2.55 RTs per individual) than healthy individuals (Shannon index = 0.79, P = 0.039; 1.97 RTs per individual, P = 0.007). Consistent with our previous study 5 , RT4, RT5, and RT8 were found to be more abundant and more prevalent in acne patients, with RT4 being statistically significantly different in relative abundance (P = 0.004), while RT2 and RT6 were more abundant in healthy individuals (Fig. S2). The strain association with acne or healthy skin is consistent with previous multi-locus sequence typing and 16S ribotype analysis of large collections of clinical P. acnes isolates 5,31,32 .
Other bacterial species frequently identified in the follicle microbiota include Staphylococcus epidermidis (0.9%), Staphylococcus capitis (0.4%), Escherichia coli (0.7%), and Clostridium sp. (0.5%). Minor bacterial taxa were found more prevalent and abundant in acne patients than in healthy individuals with varying presence and abundance (Supplementary Text). On average, acne patients had a higher diversity at the species level than healthy individuals, but not statistically significantly different (Shannon index = 0.63 and 0.44, respectively; P = 0.072). Reduced P. acnes and P. granulosum and increased minor taxa observed in acne patients suggest disruptions of the commensal skin flora in acne development.

The skin microbiome of older healthy individuals is similar to younger healthy individuals.
Acne rarely occurs in individuals over the age of 50 10,11 . The skin microbiome profiles of older individuals with clear skin can be used as independent references for healthy, acne-free skin. However, the skin metagenome of older healthy individuals has not been characterized. We performed metagenomic shotgun sequencing of the samples collected from four healthy individuals of age 55-79 (Fig. S1). The bacterial compositions of the follicular microbiome of these individuals were similar to those of the younger healthy individuals (Fig. 1D). It was previously noted that the production of sebum, a nutrient source for lipophilic bacteria, including Propionibacterium species, decreases with age 34 . In our analysis we found that the relative abundances of both P. acnes and P. granulosum were higher in older healthy individuals than in acne patients (Table 1), similar to the observations seen in younger healthy individuals. When grouping all healthy individuals together, P. granulosum remained significantly more abundant in healthy skin than acne (P = 0.001). At the strain level, P. acnes populations in older healthy individuals were similar to those of the younger healthy cohort, consisting of mostly RT1, RT2, and RT3 strains, with little or no RT4, RT5, and RT8 strains detected (Fig. S2).
When comparing all 34 healthy individuals, including the four subjects over the age of 55, with the 38 acne patients, we found an increased prevalence and abundance of P. acnes phage in the healthy group (P = 0.05) (Fig. 1A). We also observed a trend between age and the relative abundance of P. acnes phage. Higher relative abundances of phage were found more often in individuals with increased age (Fig. 1C).

Reference species
Acne (n = 38) Healthy age-matched (n = 30) Healthy 55 + (n = 4) Metagenomic composition of the skin microbiome is different between acne patients and healthy individuals. To better understand the role of the follicular microbiota in acne pathogenesis at the molecular level, we investigated the differences in metagenomic composition of the microbiome between acne patients and healthy individuals. Among the sequencing reads mapped to P. acnes, we identified 2,707 operational gene units (OGUs) in the samples, among which 1,943 OGUs (72%) were present in every sample. Rarefaction analysis indicated that the sequencing depth was sufficient to quantitatively compare the P. acnes functional profiles between acne patients and healthy individuals (Fig. S3). Since the microbiome profiles were similar between younger and older healthy individuals at both the species and P. acnes strain level, we combined the healthy individuals from both age groups in the following metagenomic analyses.
To determine whether specific metagenomic elements are associated with acne or healthy skin, we compared the relative abundances of P. acnes OGUs between acne patients and healthy individuals (Fig. S4). As the skin microbiome varies greatly among individuals 23,30,35,36 , to reduce the potential bias due to specific individuals recruited in our study, we randomly selected 100 subsets of the data, each containing two-thirds of the individuals from each clinical group (26 acne patients and 22 healthy individuals), and determined the metagenomic elements that were differentially abundant between the two groups in each random set. To capture the differences at the species level between the two cohorts, in addition to P. acnes OGUs, we also considered the species found in the samples as additional metagenomic elements and included them in the analysis. To determine the most robust set of metagenomic elements that are associated with either acne or healthy skin, we identified 63 metagenomic elements, whose relative abundances were significantly different (P < 0.05) between the two groups in at least 50 of the 100 random subsets. These 63 elements consisted of 62 P. acnes OGUs (Table S1) and P. granulosum as species.
Among the 62 P. acnes OGUs, 25 were significantly more abundant in acne patients. Two of them are involved in thiopeptide bacteriocin precursor synthesis and transport (PAGK2104 and PAGK2105, as annotated in P. acnes HL096PA1 genome). Thiopeptide bacteriocins belong to a family of microcins, which are produced by Gram-positive bacteria and inhibit the growth of other Gram-positive species by blocking protein translation 37 . Additionally, 19 of the 25 acne-associated OGUs were from locus 2 (PAGK0160-PAGK0178), a genomic island previously identified mainly in RT4 and RT5 strains and highly associated with acne 5,20 . Locus 2 spans over 20 Kb and encodes 23 ORFs (Fig. S5). The differentially abundant locus 2 OGUs identified include a number of genetic elements involved in recombination, such as a single-strand binding like protein (Ssb), shown in other bacterial species to be involved in chromosomal transformation 38 , and resolvase-like protein, previously implicated in genetic integration. Other OGUs include a cluster of Streptolysin S-associated genes (sag) involved in the biosynthesis and transport of a bacterial toxin. Identified and characterized in Streptococcus pyogenes, sag genes are involved in synthesis, post-translational modification, and transport of a ribosomally synthesized bacteriocin, which has been linked to antimicrobial activity as well as invasive infections 39,40 . The presence of genes homologous to sagB, C, and D in locus 2 suggests involvement of this locus in bacteriocin biosynthesis and maturation. Additionally self-immunity and transport genes are found immediately downstream. Other OGUs in this locus have putative roles in cell viability, virulence, and immunity including ATP-binding cassette (ABC) transporter and ABC-binding protein for translocation of lipids, nutrients and/or toxins, CAAX amino protease, which is thought to be involved in self-immunity 41 , and partitioning machinery needed for cell replication and division. While the specific functions of genes in this locus in acne are unclear, the absence of locus 2 in healthy individuals and a high abundance in acne patients provides strong evidence for its association with acne.
In addition to locus 2, in our previous studies, two other genomic loci (locus 1 and locus 3) were found enriched in acne-associated RT4 and RT5 strains 5,19,20 . To examine whether these three loci were overrepresented in the metagenome of acne patients, we plotted the relative abundance ( Fig. 2A), the fold change between acne patients and healthy individuals (Fig. 2B), and the prevalence ratio ( Fig. 2C), of each OGU encoded in these three loci across all individuals. We found a higher average relative abundance and prevalence of all three loci in acne patients compared to healthy individuals (Fig. 2). Locus 2 was rarely found in the microbiome of the healthy individuals, and was significantly associated with acne (P = 0.002) (Fig. 2). Since most RT4 and RT5 strains harbor locus 2 and are resistant to antibiotics 5,19 , we examined the treatment history of each individual to determine whether the presence of locus 2 was due to acne treatment. While locus 2 was found in some treated acne patients (n = 8), it was absent in other treated patients (n = 8) and was present in many untreated patients (n = 10). The presence of locus 2 was not significantly different between treated and untreated patients (Fisher's exact test, P = 1), suggesting that locus 2 is a characteristic of the disease rather than treatment ( Fig. 2A). Additional analysis to further support the association of locus 2 with the disease is described in the supporting text ( Fig. S6; Supplementary Text).
Of the 62 differentially abundant P. acnes OGUs, 37 OGUs were more abundant in healthy individuals. In contrast to the enrichment of virulence-related genes observed in the acne metagenome, genes involved in microbial metabolism and nutrient biosynthesis were significantly more abundant in the healthy metagenome (Fig. S4). Examples include glycosyl transferase (PAGK0136), D-alanine-D-alanine ligase (PAGK0821), and cobalamin-independent methionine synthase (PAGK1035), which are involved in polysaccharide, cell wall, and amino acid biosynthesis, respectively. An enrichment of these genes in the healthy metagenome is consistent with the metagenomic study by Mathieu et al. 22 , which suggested a functional role of the resident bacteria in healthy skin in the exploitation of compounds from the human skin including sugars, lipids, and iron 22 . Other significantly more abundant OGUs in the healthy metagenome include glycerol uptake facilitator protein (PAGK2214), which aids in carbohydrate metabolism processes by transporting glycerol across the cytoplasmic membrane.
Recently it was shown that fermentation of glycerol by P. acnes contributes to skin and host health via the production of short chain fatty acids (SCFA) 42 . SCFA including acetic acid, lactic acid, and propionic acid were shown to significantly decrease the colonization of skin-associated pathogens such as methicillin-resistant Staphylococcus aureus 42 .
Scientific RepoRts | 6:39491 | DOI: 10.1038/srep39491 The balance between acne-and health-associated metagenomic elements shapes the host skin microbiota in acne and health. Our observations of differentially abundant species, strains, and metagenomic elements between the skin microbiome of acne patients and healthy individuals led us to hypothesize that the balance between acne-and health-associated metagenomic elements determines the virulence and health properties of the microbiota in skin disease and health. We define the balance as the ratio between the relative abundances of acne-and health-associated metagenomic elements. When the relative abundances of the 62 P. acnes OGUs and P. granulosum from all individuals were compared, we found that this ratio in acne patients was significantly higher than in healthy individuals (P = 2.9 × 10 −5 ). Figure 3 illustrates the relative abundances of these 63 metagenomic elements across all 72 individuals. The metagenomic profiles of healthy individuals were readily distinguishable from acne patients harboring locus 2. Acne patients with locus 2 had significantly higher relative abundances of acne-associated OGUs (in 24 of 25 OGUs P = 0.00024-0.021, and in 1 of 25 OGUs P = 0.083) (Fig. 3A). However, these subjects only represented about half (47%) of the patients recruited in our study. When we examined the metagenomic profiles of the other acne patients without locus 2, we found that they were distinct from healthy individuals with decreased relative abundances of health-associated P. acnes OGUs (in 23 of 37 OGUs P = 0.0024-0.046, and in 14 of 37 OGUs P = 0.058-0.30). We were able to reveal distinct microbiome profiles between acne patients and healthy individuals that were not readily apparent at the taxonomic level, with differences in the relative abundances of the metagenomic elements that were associated with either acne or healthy skin (Fig. 3).
In addition to P. acnes OGUs, we found that the relative abundances of P. acnes and P. granulosum were also important factors influencing the clinical state of the skin. As an example, an acne patient in our cohort (labeled with † on Fig. 3) displayed a profile of the 62 P. acnes OGUs that was more similar to the healthy individuals than all other acne patients (P = 5.29 × 10 −5 ). However, the observed low relative abundances of both P. acnes (78% compared to the average of 91%) and P. granulosum (0.070% compared to the average of 1.6%) may contribute to the acne status of this patient. This suggests that a healthy follicular microbiota not only requires health-associated P. acnes strains and genetic elements, but also requires significant dominance of resident propionibacteria. Taken together, our findings suggest that the balance between skin metagenomic elements determines the virulence and health properties of the skin microbiota and is important in skin health and disease. The 74 OGUs from locus 3, which is a plasmid, are also shown. Each row represents an individual. Acne patients (n = 38) and healthy individuals (n = 34, including those with age over 55) were compared. Individuals within each group were clustered based on the average relative abundance of locus 2 OGUs. Ribotype composition and past and current acne treatments are indicated on the right. Multiple treatments are depicted by more than one color. (B) Fold changes in relative abundance of the OGUs in loci 1, 2, and 3 between acne patients and healthy individuals. Acne-associated OGUs had a fold change > 1, while health-associated OGUs had a fold change < 1. (C) Prevalence ratio of the OGUs in loci 1, 2, and 3 between acne patients and healthy individuals. The presence of an OGU in a sample is defined as an OGU with at least 1X coverage after normalization.
We further investigated whether the relative abundance profiles of the metagenomic elements are sufficiently robust to correctly classify the clinical state of skin samples. We performed a supervised class prediction analysis based on a modified weighted gene voting algorithm 43,44 (Supplementary Text). We generated 1,000 permutations. In each permutation, samples were randomly assigned to either acne or healthy state, with 38 samples in the acne group and 34 samples in the healthy group. We identified differentially abundant metagenomic elements between the two groups among all but one sample, and predicted the clinical state of the withheld sample based on the abundance profiles of the metagenomic element, known as the leave-one-out cross-validation (LOOCV). With the use of the clinically defined acne-healthy grouping, the algorithm predicted the clinical states of 49 out of 72 samples (68%) with a prediction strength threshold of 0.25. Thirty-four of the 49 samples were assigned correctly (69% accuracy). The number of correctly assigned samples based on the clinical grouping was higher than most of the permutated groupings (P = 0.064). The clinical grouping also had a significantly higher prediction accuracy (69%) than all but one of the permutated groupings (P = 0.001) (Fig. 4A).
We further improved our classifier by using the 63 metagenomic elements, which are the most robust set based on the 100 random samplings as described earlier. Since the 19 OGUs from locus 2 had similar abundance profiles across all samples, to avoid overweighing locus 2 in the classification, we combined these 19 OGUs and used their average relative abundance in the classifier. The refined classifier therefore consisted of the abundance profiles of 45 metagenomic elements: P. acnes OGUs/loci, and P. granulosum. Based on this refined set, we were able to assign the clinical states of 31 of the 38 acne patients (82%, prediction strength threshold of 0.25). Twenty-four acne patients were correctly assigned (accuracy of 77%). Among the healthy individuals, we were able to assign 21 of the 34 subjects (62%) with 20 correctly assigned (accuracy of 95%). Overall, the classifier was able to assign 72% of the subjects with an accuracy of 85% ( Fig. 3B; Fig. 4B).
Furthermore, to validate that these metagenomic elements can be used as markers for clinical classification, we collected an "independent sample set" from ten additional subjects, including 4 acne patients and 6 healthy individuals, one of which was over 50 years old. We used the refined 45 metagenomic elements to predict the clinical state of each independent sample (Fig. 4B). Our classifier was able to assign the clinical states of 7 samples (70%) with an accuracy of 86%, highly consistent with the classification results from the training sample set of 72

Figure 3. The relative abundances of acne-and health-associated metagenomic elements in acne and healthy individuals. (A)
The relative abundances of 62 P. acnes OGUs, including 25 acne-and 37 healthassociated OGUs, and three organisms associated with healthy skin, P. acnes, P. granulosum, and P. acnes phage, were plotted for each individual to illustrate the importance of a balance between these metagenomic elements in health and acne. Each column represents an individual, and each row represents an OGU or an organism. The top ten ribotype composition and acne severity score (acne patients only) of each individual are also shown. samples. Based on this, we conclude that the metagenomic elements identified in our study can be used to classify the clinical state of the skin with high accuracy.

Discussion
The role of the human cutaneous microbiota in skin health and disease is not yet fully understood. In acne, the dominant bacterial species, P. acnes, has been believed to have a causal role 15,16 . However, the dominance of this species on healthy skin with a role in preventing the colonization of skin pathogens has long been a concern when trying to establish a link between P. acnes and acne pathogenesis 5,45,46 . The distinction in acne or health associations among different lineages of P. acnes strains was elucidated recently 5,19,20 and partially addressed this concern. However, it is still unclear what impact on skin health or disease the skin microbiota exerts when multiple P. acnes strains coexist in the community.
In this study, we performed ultra-deep metagenomic shotgun sequencing of the samples collected from acne patients and young and older healthy individuals to quantitatively compare the microbiome differences between disease and health at the metagenomic level. To our knowledge, this is the first study in which the skin metagenome in a diseased state as well as in older healthy individuals has been analyzed. With a relatively large number of subjects, our ultra-deep shotgun sequencing provided a high coverage of the metagenome (equivalent to an average of 422X coverage of the P. acnes genome per sample), which allowed quantitative characterization of the skin microbiome. We discovered that while the skin microbiome was highly variable among individuals, there were significant differences in the relative abundances of certain species, strains, and metagenomic elements between healthy and diseased states.
We revealed a trend of higher relative abundances of P. acnes and P. granulosum in healthy individuals compared to acne patients ( Fig. 1D; Table 1). This is contrary to what has been long believed and suggests a potential probiotic role of these two species in maintaining healthy skin. Early studies suggested the involvement of both P. acnes and P. granulosum in acne pathogenesis because of frequent isolations of these species from the follicles of acne patients [47][48][49] . More recently, however, using immuno-fluorescence microscopy, P. acnes and P. granulosum were identified in the follicles of healthy subjects at a similar prevalence to that of acne patients 50 . Our finding of a higher relative abundance of these species in healthy skin suggests that P. acnes and P. granulosum are essential members of the healthy skin microbial community. A beneficial role of propionibacteria in the skin microbiota is consistent with skin microbiome studies of other skin diseases, such as atopic dermatitis and psoriasis, in which the abundance of propionibacteria was reduced in the diseased states and increased after treatment 3,6,51 .
The high relative abundances of P. acnes and P. granulosum observed in healthy individuals suggest a role for these species in skin health. Propionibacterium species are thought to confer health benefits via the production 25. This result is statistically significant (P = 0.001), because only one of the 1,000 permutated groupings had a higher accuracy. However, that particular grouping (accuracy of 72%) had fewer samples assigned than the clinical grouping (n = 39 vs 49). This demonstrates that the differences in the relative abundances of the metagenomic elements between acne patients and healthy individuals can be used to predict the clinical states of the skin. When we used the refined set of 45 metagenomic elements, including 43 P. acnes OGUs, P. acnes locus 2, and P. granulosum, we further improved the prediction accuracy of the training sample set to 85%. (B) Consistent with the prediction accuracy on the training sample set, for the independent sample set (n = 10), the 45 metagenomic elements were able to assign 70% of the samples with 86% accuracy.
Scientific RepoRts | 6:39491 | DOI: 10.1038/srep39491 of SCFA. SCFA possess antimicrobial properties and contribute to the acidic pH of the skin in defending against pathogens 42,52,53 . Disruption in the normal skin pH has been linked to several skin diseases, including atopic dermatitis, ichthyosis, fungal infections [54][55][56][57] , and acne 58 . As propionibacteria play important roles in skin health, our finding suggests that effective acne treatments should not aim to kill all Propionibacterium in the follicle, as practiced in most of the current acne treatments, but rather to target pathogenic strains and keep or supplement the beneficial strains to maintain a healthy state of the skin.
Compared to P. acnes, much less is known about the role of P. granulosum in skin health and disease. Limited genomic information exists for P. granulosum. Previous comparative analysis based on two draft genomes revealed that, compared to P. acnes, P. granulosum lacks virulence-related factors such as CAMP factors and sialidases, and exhibits no neuraminidase and hyaluronidase activities 26 . The bacterium may provide protection to the skin with less production of virulence-associated elements. Further study to elucidate the potential health benefits of P. granulosum in the skin microbiota is warranted.
In the analysis of the skin microbiome in healthy individuals, we revealed, for the first time, a positive correlation between P. acnes phage abundance and subject age as well as a link between phage abundance and healthy skin (Fig. 1C). This finding potentially could explain in part why the prevalence of acne declines with age 10 . The observed reduction of acne prevalence in older individuals 10,11 may be a result of increased abundance of P. acnes phage, in addition to changes in the ecological niche such as reduced sebum and thus reduced propionibacteria load in the follicle 34 . Our findings are consistent with previous culture-based observation of a higher frequency of P. acnes phage isolated from healthy individuals than acne patients 29 , suggesting a role for P. acnes phage in acne-free skin, potentially by modulating P. acnes strain populations in the follicular microbiota. Despite being utilized for almost a century in Eastern Europe as a treatment for acne and other diseases 59 , few studies have been performed to understand the pray-predator interactions and dynamics between Propionibacterium species/ strains and P. acnes phage populations in the skin microbial community 29 . The observation of increased phage abundance in healthy individuals merits further investigation of the role of P. acnes phage in skin health. Our findings suggest that it is potentially promising to design personalized and strain-specific phage-based treatment that can target specific P. acnes strains and thus shift the strain composition to a healthy skin microbiota 29 .
To understand the role of the skin microbiota in skin health and acne at the molecular level, we identified a robust set of metagenomic elements that are differentially abundant between the two groups and can be used as markers for classification of the clinical states of the skin. In our analysis, the clinical states of most of the individuals were accurately classified based on the 45 metagenomic elements, including the training set samples from 72 subjects and the 10 independent samples not used in the identification of the metagenomic markers. Given the large individual variations in the skin microbiome, and that acne is a multi-factorial disease with bacteria accounting for only one of the mechanisms, our finding that the metagenome of the follicular microbiota can be used to classify the host skin states is significant. Furthermore, our results suggest that it is promising to develop reliable markers based on the skin metagenome to improve diagnosis, skin health monitoring, and disease prognosis.
Among the 72 subjects in our study cohort, 20 could not be assigned with confidence using the classifier. One explanation is that acne is a disease with a wide spectrum of severity and that the skin microbiome is reflective of this large variation. Although individuals presented with acne or healthy skin at the time of sampling, the follicular microbiome might have been in the transition between the two clinical states. In support of this theory, we observed that the patients with mild acne (with acne score 1-2) were often not assigned with confidence or incorrectly assigned by our classifier. On the other hand, the majority of the patients with moderate or severe acne (with acne score 3-5) were assigned with high accuracy (Fig. 3). Consistently, in our independent validation sample set, two of the four acne patients who had a low acne score of "1" could not be assigned with confidence, while the other two patients with scores of "2" and "4", respectively, were correctly assigned (Fig. 4B). Additionally, acne is a multi-factorial disease. Other factors including environment, host genetic predisposition, age, hormone status, and life style are also thought to play a role in acne pathogenesis [60][61][62][63] . Better understanding of these factors will help improve the classification of the disease.
Our findings from the metagenomic shotgun analysis of the follicular microbiome suggest that the balance between acne-and health-associated species and metagenomic elements of the skin microbiota contributes to skin health and disease. We observed that when less acne-and/or more health-associated microbial elements were present, an individual had healthy skin. On the other hand, when the balance shifted towards more acne-associated elements, the disease state prevailed. This concept of a disrupted balance in the metagenomic elements of the microbiota contributing to disease, and potentially disease severity, is largely different from the traditional definition of microorganism-associated diseases. Diseases associated with microorganisms are typically linked to the presence of a particular species or strains, ignoring other members of the resident community 64,65 . In this study, we raise a new viewpoint of microbial pathogenesis in acne, whereby the pathogenesis is not due to the presence of one particular species or strain but rather the balance of the overall microbial community, which leads to skin health or the development of acne. The mere presence of a disease-associated species or strain or genetic element may not necessarily confer the disease, depending on the commensal members of the community. This balance-based model for microbial pathogenesis fits well for acne, a prevalent multi-factorial disease that exhibits a wide spectrum of severity amongst the general population. We believe that this new viewpoint based on the balance of the microbiota will provide novel insights on other similar microorganism-associated diseases. Additionally, our findings suggest potential future development of personalized and targeted therapies in the treatment of acne and the maintenance of skin health by supplementing the skin microbiota with probiotic organisms to shift the balance toward a healthy microbiome.

Materials and Methods
Subjects. Similar to that previously described 5 , in this study, subjects with acne or healthy skin were recruited from various clinics in Southern California, USA, including private practice, managed care, and public hospital Scientific RepoRts | 6:39491 | DOI: 10.1038/srep39491 settings, as well as outside of dermatology clinics, to best represent the diversity of populations and history of medical care. The diagnosis of acne was made by board-certified dermatologists. The presence of acne was graded on a scale of 0 to 5 according to the Global Acne Severity Scale 66 . Grades were recorded for both the face and the nose separately, where zero represents healthy skin and 5 represents the most severe inflammatory cystic acne. The presence of scarring was also noted. Subjects with healthy skin were determined by board-certified dermatologists and were defined as people who had no acneiform lesions on the face, chest, and back. They were excluded if they had other skin conditions that the investigators felt would affect sampling or the microbial population on the skin. Additionally, healthy subjects were excluded if they had been previously treated for acne. Among 72 subjects recruited, 38 were acne patients with an average age of 24 years (14-37 years; Fig. S1) and had an average acne score on the face and nose of 2.4 (range 1-5) and 0.6 (range 0-2), respectively. Of the 34 healthy individuals, 30 were age-matched, with an average of 26 years old (16-38 years; Fig. S1), and four were over the age of 55, with an average of 67.5 years (55-79 years; Fig. S1). The subjects responded to a written questionnaire, administered by a physician or a well-trained study coordinator who went over each question with the subjects. Of the acne patients, 16 received acne treatments during or prior to the study, including antibiotics, benzoyl peroxide, and topical retinoids (Fig. 2). Clinical information of the study participants and the histograms of age distribution of acne patients and healthy individuals are shown in Figure S1. All subjects provided written informed consent. All protocols and consent forms were approved by both the UCLA and Los Angeles Biomedical Research Institute IRBs. The study was conducted in adherence to the Helsinki Guidelines.
Sample collection and genomic DNA extraction. Sample collection and processing were performed as previously described 5 . Briefly, skin follicle samples were taken from the nose of the subjects using Bioré Deep Cleansing Pore Strip (Kao Brands Company, Cincinnati, OH) following the manufacturer's instructions. Clean gloves were used for each sampling. To avoid sample cross-contamination, strict sterile working conditions were enforced, including appropriate lab-wear and routine sterilization of the working surfaces and equipment. After being removed from the nose, the strip was immediately placed into a 50 ml sterile tube and kept on ice or at 4 °C. Samples were processed within 24 hours of collection. Individual follicular plugs were manually picked from the adhesive strip with sterile forceps and placed into 2 ml sterile microcentrifuge tubes filled with ATL buffer (Qiagen) and glass beads (0.1 mm diameter) (BioSpec Products Inc., Bartlesville, OK). Cells were lysed using a beadbeater for 3 minutes at 4,800 rpm at room temperature, then centrifuged at 14,000 rpm for 5 minutes. The supernatant was retrieved and used for genomic DNA (gDNA) extraction using QIAamp DNA micro kit (Qiagen). The manufacturer protocol for extracting genomic DNA from chewing gum was used. DNA extraction reagents were routinely tested for potential contaminations using negative control extractions. Concentration of gDNA was determined using Qubit Fluorometric Quantitation (Invitrogen). Concentrations from negative control experiments were below the detectable range.
Library construction and metagenomic shotgun sequencing. Genomic sequencing libraries were prepared using NexteraXT kit (Illumina). Briefly, 1 ng of extracted metagenomic DNA was tagmented using transposase technology. 5′ and 3′ NexteraXT dual-indices were added to uniquely barcode each library. Indexed libraries were amplified using the limited 12-cycle PCR program as instructed by the manufacturer's guidelines. Libraries were purified with Agencourt AMPure XP magnetic beads (Beckman Coulter). Library quality and average fragment length were assessed using Bioanalyzer (Agilent Technologies). Libraries were quantified with KAPA library quantification kit (KAPA Biosystems), according to the manufacturer's instructions. Finally, libraries were randomly pooled together and sequenced using MiSeq and/or HiSeq platforms (Illumina) with paired-end reads of 251 bp or 101 bp, respectively. Following sequencing, reads were de-multiplexed.
Data cleaning and sequencing depth analysis. Data cleaning of the metagenomic shotgun sequences was performed following the protocol used in the HMP 21 . Briefly, low quality reads and human DNA reads were filtered out first. Human reads accounted for 43% of the sequencing data. In total, 78 Gbp of cleaned, host free sequencing data were obtained for 72 samples. The average number of mappable reads per sample was 7.2 × 10 6 (4.0 × 10 5 -4.7 × 10 7 reads), accounting for 72.6 ± 3.0% of the cleaned, host free data. These data were used in the downstream analyses. To ensure adequate sequencing coverage, we performed sequencing depth analyses. Rarefaction curves were plotted, demonstrating sufficient sequencing depth of P. acnes OGUs for functional profiling (Fig. S3).

Taxonomic composition analysis.
We determined the taxonomic composition of the microbiome by mapping metagenomic shotgun sequences against microbial reference genomes, using a method similar to that described by Schloissnig et al. 67 . In addition to the HMP reference genomes, we included additional Propionibacterium genomes and P. acnes phage genomes. Fungal genomes from NCBI non-redundant nucleotide database (RefSeq release 48) were also included. Bowtie2 was used in mapping the shotgun reads with 80% identity threshold 68 . The relative abundance of each taxon in each sample was calculated by counting the number of base pairs covering the genome of the organism, normalized by the genome size and the sequencing depth of the sample. To identify common taxa in the population that were either associated with acne or healthy skin, we performed statistical analysis on only the species that were identified in at least 20% of the individuals of our study cohort. P. acnes pan-genome and OGUs. P. acnes pan-genome was constructed based on the genomes of 80 P. acnes strains, as described by Tomida et al. 19 . The set of P. acnes OGUs was constructed similarly as described by Hansen et al. 69 with minor modification. Using CD-HIT 70 , genes in P. acnes genomes were binned based on their nucleotide sequences with 80% identity threshold. The relative abundance of each OGU was calculated as the total base pair coverage within the OGU region normalized by the OGU length and the sequencing depth of P. acnes genomes in each sample. The presence of an OGU in a sample was defined as more than 1X coverage after normalization.
Scientific RepoRts | 6:39491 | DOI: 10.1038/srep39491 Functional classification of P. acnes OGUs. P. acnes OGUs were annotated with KEGG orthologous groups (KOs) by mapping them to the KEGG database 71 (version 54; e-value < 10 −5 ) using BLAST, in a procedure similar to that described by Shi et al. 72 . The relative abundance of each functional category in each sample was calculated by summing all the functional genes involved in each functional category.

Identification of differentially abundant OGUs and species.
To minimize the potential bias due to a few individuals in the acne and healthy groupings, we used a resampling approach in identifying differentially abundant OGUs and species between acne patients and healthy individuals. We randomly selected two thirds of the samples in each group (26/38 in the acne group and 22/34 in the healthy group) and compared them to identify metagenomic elements that were significantly different in relative abundance between the two groups (P < 0.05, Student's t-test). We repeated this sub-sampling and comparison 100 times and identified the OGUs and species that were differentially abundant in at least 50 of the 100 samplings.
Classification of clinical states based on the metagenomic profile. The supervised classification was performed using the weighted gene-voting algorithm with minor modification and leave-one-out cross-validation as described by Golub et al. 43 and Bleharski et al. 44 . The top 25 differentially abundant metagenomic elements were used for leave-one-out classification in each permutation. More details of the method are described in the Supplementary Text. Independent sample set for validation. An additional ten subjects were recruited and sampled, using the same sampling method described above. Four samples were collected from acne patients (average age 24.5 years), and six samples were collected from individuals with healthy skin: five age-matched subjects (average age 33.4 years) and one older subject (77 years old). Samples were sequenced to a comparable sequencing depth (average of 8.04 × 10 8 bp per sample). The profiles of the 45 metagenomic elements identified from our study cohort were determined for each sample. Profiles based on these 45 metagenomic elements were used to classify each sample (Supplementary Text).

Statistical analysis.
Statistical analyses were performed to calculate the differences between acne patients and healthy individuals at the species and strain levels using the Wilcoxon Rank-Sum Test (U-test). We corrected for multiple testing using the Bonferroni correction. Student's t-test with two-tailed distribution was used in statistical testing of the differences in the relative abundance of P. acnes OGUs between the two groups. Pearson's correlation was used to calculate the correlation co-efficient. Data Availability. The sequence data from this study have been submitted to NCBI BioProject (http://www. ncbi.nlm.nih.gov/bioproject) under BioProject number 283427.