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Rap1p telomere association is not required for mitotic stability of a C3TA2 telomere in yeast

Telomeric DNA usually consists of a repetitive sequence: C1–3A/TG1–3 in yeast, and C3TA2/T2AG3 in vertebrates. In yeast, the sequence‐specific DNA‐ binding protein Rap1p is thought to be essential for telomere function. In a tlc1h mutant, the templating region of the telomerase RNA gene is altered so that telomerase adds the vertebrate telomere sequence instead of the yeast sequence to the chromosome end. A tlc1h strain has short but stable telomeres and no growth defect. We show here that Rap1p and the Rap1p‐associated Rif2p did not bind to a telomere that contains purely vertebrate repeats, while the TG1–3 single‐stranded DNA binding protein Cdc13p and the normally non‐telomeric protein Tbf1p did bind this telomere. A chromosome with one entirely vertebrate‐sequence telomere had a wild‐type loss rate, and the telomere was maintained at a short but stable length. However, this telomere was unable to silence a telomere‐adjacent URA3 gene, and the strain carrying this telomere had a severe defect in meiosis. We conclude that Rap1p localization to a C3TA2 telomere is not required for its essential mitotic functions.