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High-resolution holographic imaging technology by microscopic image plane holography

The imaging technology of digital microscopic image plane holography (DMIPH) is studied in this paper. The point spread function expression and the recording conditions of DMIPH system are derived. The quadratic phase factor which introduced by the microscope objective lens can be eliminated through choosing the proper position of the reference point source when the hologram is recorded by spherical reference waves. By using plane waves and spherical waves as reference waves respectively two image plane holographic recording systems are built. Using a USAF test target as microscopic object, the recorded digital holograms are reconstructed by angular spectrum algorithm. The experimental results show that in the case of spherical reference waves if the distance from the equivalent of lighting point source to CCD plane is equal to the distance between the reference point source and CCD plane the quadratic phase distortion introduced by the microscope objective lens can be removed and that DMIPH is superior to common digital holographic microscopy.