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Influence of cholesterol and unsaturated fatty acids on [125I]hCG binding to rat testicular membranes.

Incubation of testicular membranes with cholesteryl-hemisuccinate resulted in an increase of both the membrane microviscosity and [125I]hCG specific binding. 14C-labelled cholesterol as well as fatty acids were effectively incorporated into membrane preparations. Insertion of unsaturated fatty acids (cis-vaccenic, linoleic, oleic, linolenic and arachidonic) into the membrane decreased its microviscosity under simultaneous disappearance of [125I]hCG specific binding. The latter phenomenon seems to be caused by an increase of nonspecific binding which was not due to the uptake of radio-activity from labelled gonadotropin by fatty acids or double bonds of fatty acids incorporated into testicular membranes or taken up by a resin Amberlite IRA-400. The disappearance of specific binding of [125I]hCG by the fluidizing action of unsaturated fatty acids can be reversed by the subsequent action of cholesterol.