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Synthesizing t-2 toxin conjugates with proteins for the use in immunosensitive test systems

We have devised the methods for synthesizing conjugates of mycotoxin T-2 with the proteins of bovine serum albumin, ovalbumin, hemocyanin and gelatin for producing antigens, subsequent immunizing animals, and obtaining specific antibodies. We have proved that T-2 toxin conjugates with bovine serum albumin, ovalbumin and hemocyanin are insoluble in water and are suitable only for the immunization of animals, but cannot be used in immunochemical reactions. Only a T-2 toxin-gelatin conjugate is soluble in water and can be used in immunochemical reactions. Availability of a T-2 toxin-bovine serum albumin conjugate is proved by the method of a thin-layer chromatography. We have obtained antibodies to T-2 toxin after immunization of rabbits with a T-2 toxin-bovine serum albumin conjugate. A T-2 toxin-gelatin conjugate is used for immobilization on a solid surface of an immunosensor with the effect of a surface plasmon resonance. Antisera with an antibody titer of 1:5000 were selected to calculate the concentration of T-2 toxin by the specified method on the basis of competition. The proven methods for obtaining conjugates, producing immune antisera and identifying antibodies can be the basis for domestic biosensor immunodiagnostic test systems.