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Isolation of chlorogenic acid from Mutellina purpurea L. herb using high-performance counter-current chromatography

The aim of the study was to explore proper isolation conditions of chlorogenic acid from the herb of Mutelina purpurea L. – a new source of this bioactive molecule. The accelerated solvent extraction (ASE) with 40% aqueous solution of methanol combined with high-performance counter-current chromatography (HPCCC) was utilised for the efficient extraction and the separation of chlorogenic acid from the M. purpurea herb in less than 30 min. The structure of the obtained compound was confirmed by mass spectrometry and NMR analysis. The preparative HPCCC was performed using the mixture of ethyl acetate, butanol and water (4:1:5, v/v/v) in the reverse-phase mode. The chlorogenic acid was isolated from this herb for the first time, yielding 96% purity. The ASE with 40% methanol combined with HPCCC separation was proven to be a useful tool for quick and efficient isolation of chlorogenic acid from M. purpurea.