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The Effect In vitro of High‐Density Lipoprotein from Healthy and Infected Humans on the Oxidative Metabolism of Polymorphonuclear Leukocytes

We studied the effects in vitro of high‐density lipoprotein from healthy (N‐HDL) and from infected humans (AP‐HDL) on the oxidative metabolism of human polymorphonuclear leukocytes (PMN). Products of the H2O2–MPO–halide system were monitored by luminol‐enhanced chemiluminescence and superoxide anion formation was monitored by lucigenin‐enhanced chemiluminescence during stimulation of human PMN with phorbol myristate acetate (PMA) or an opsonized stimulus (OS). The results showed that N‐HDL and AP‐HDL affect the oxidative metabolism of PMN in different ways. The posible role of this effect is discussed. © 1997 John Wiley & Sons, Ltd.