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Preparation of Dicyclohexylamine Alpha Acids and Dicyclohexylamine Beta Acids

The current HPLC calibration standard for measuring alpha acids and beta acids in hops and hop products is International Calibration Extract Three (ICE-3), a deoiled CO2 hop extract. Laboratories that use ICE-3 must warm and mix the calibration extract to insure that it is homogenous prior to use. Alternatively, one can prepare crystalline dicyclohexylamine salts of alpha acids (DCHA-alpha acids) and beta acids (DCHA-beta acids), which are easier to use and more stable than ICE-3.