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Isolation of Tahyna virus from biting midges (Diptera, Ceratopogonidae) in Czecho-Slovakia.

A total of 14,250 haematophagous biting midges (genus Culicoides, Diptera: Ceratopogonidae) were collected in June 1986 in the mountains Ceskomoravská vysocina (about 600 m above sea level); from these 2 strains of Tahyna virus (serogroup California, Bunyaviridae) were isolated. To our knowledge this is the first isolation of a California serogroup virus from the species of family Ceratopogonidae as well as the first report of arbovirus isolation from biting midges in Europe.