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Disposition of cocaine and norcocaine in blood and tissues of B6C3F1 mice.

The biodisposition of cocaine and norcocaine in blood and tissues of immunological importance in B6C3F1 mice following exposure to cocaine or cocaine plus an organophosphate esterase inhibitor, diazinon, is presented. Analysis of specimens was by gas chromatography-mass spectrometry. Results from these studies indicate that pretreatment with diazinon significantly increases cocaine and norcocaine concentrations in the blood, spleen, thymus, and liver. Following acute exposure to cocaine-diazinon, cocaine was found in the spleen and thymus up to 1 hour after exposure. Norcocaine was not detected at this time. Following 7-day exposure to cocaine-diazinon, both cocaine and norcocaine were found in liver, blood, and spleen up to 1 hour after the last exposure; however, only cocaine was detected in the thymus at 1 hour. Cocaine and norcocaine were not detected in any tissues 24 hours after the last exposure.