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[Determination of phospholipid in Cibotium barometz by supercritical fluid extraction and RP-HPLC].

A supercritical fluid extraction (SFE) method was used to extract phospholipids in Cibotium barometz. After a homogeneous design experiment, SFE conditions were obtained which were pressure at 31.5 MPa, temperature at 46.2 degrees C and a 0.37 L/kg modifier in supercritical fluid. The sample was extracted for 2 hours. A RP-HPLC method was developed for the determination of phosphatidylcholine. RP-HPLC conditions included a RP-HPLC column packed with Waters Symmetry C18(5 microns), a mobile phase composed of methanol-1% phoshporic acid(90:10) and the detection wavelength at 206 nm. The content of phosphatidylcholine was 0.198%(RSD 4.2%), and the method can be a measure of quality control for Cibotium barometz and its preparations.