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Performance evaluation of a novel reticulocyte identification method that uses metachromatic nucleic acid staining based on a crossover analysis of emission DNA/RNA light (RNP Determination™) in hematology analyzer Celltac G+

Assessing the percentage of reticulocytes (%Retic) is useful for diagnosing and treating blood diseases that present with anaemia. The Celltac G+™ hematology analyzer (HA) uses a novel reticulocyte identification method that involves metachromatic nucleic acid staining with acridine orange and crossover analysis of emission light of DNA/RNA (determination of red cells, nucleic acid‐containing cells, and platelets, RNP Determination™). The red and green fluorescence generated by stained single‐stranded RNA and double‐stranded DNA express immaturity and morphological abnormality of erythrocytes by detecting erythrocyte RNA and DNA content.