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Effects of storage conditions on lymphocyte phenotypes from healthy and diseased persons

Anticoagulant and room temperature storage of peripheral blood was evaluated in healthy and diseased persons (AIDS and CLL) for its effect upon lymphocyte phenotyping by flow cytometry. Whole blood was stored in either Li heparin, EDTA, or ACD for up to 96 hours. Aliquots at 5 time intervals were evaluated using CD3, CD2, CD4, CD8, and CD19 monoclonal antibodies.