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Studies on Bermuda grass pollen allergens.

Two allergens, BGP-1 and BGP-2, were identified in crude Bermuda grass pollen extract. BGP-1 was a major allergen and elicited a skin reaction in all subjects sensitive to the crude extract, whereas BGP-2 was a minor allergen and elicited a reaction in only 75% of such subjects. Calibrated column chromatography was used to determine the molecular weights and Stokes radii of the allergens. For BGP-1 they were 30 000 daltons and 24,3 X 10(-8) cm respectively and for BGP-2 they were 14 000 daltons and 18,1 X 10(-8) respectively. The sedimentation coefficient (S20,w) of BGP-1 on sucrose density gradient ultracentrifugation was 3,1 and on iso-electric focusing its iso-electric point was found to be pH 5,4. The reason why only certain individuals sensitive to BGP-1 are sensitive to BGP-2 is discussed. It is possible that the former is a dimer of the latter.