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Identification of functional regions of the positively acting regulatory gene amdR from Aspergillus nidulans

The amdR (intA) regulatory gene of Aspergillus nidulans encodes a 765‐amino‐acid polypeptide which determines the omega‐amino acid induction of at least five structural genes. The AmdR polypeptide contains a potential Zn(ll)2Cys6 DNA‐binding motif which has been shown to be present in the N‐terminal region of a large number of fungal activator proteins. In vitro mutagenesis of the fourth cysteine of this motif abolishes AmdR function as shown by loss of complementation of an amdR− mutation and by the AmdR− phenotype of a mutant gene replacement strain. Studies using constructs in which the proposed AmdR DNA‐binding motif is replaced with that from another activator, FacB, shows that induction is independent of DNA‐binding specificity and that sequences in the C‐terminal region of AmdR are activation domains. Sequencing of several amdR mutant alleles which affect activation and/or induction, together with studies of deletion constructs indicate that changes in the conformation of the protein determines its activity and that this is modulated by inducers.