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Automated Method for Tracking Individual Red Blood Cells Within Capillaries to Compute Velocity and Oxygen Saturation

Objective: The authors present a new method to track individual red blood cells (RBCs) as they move through capillaries. This method uses a recently developed Measurement and Analysis System for Capillary Oxygen Transport (MASCOT) and the concept of space–time images to track RBCs between consecutive frames of video recordings of the microcirculation.