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The Potential of HUMACTBP2 (SE33) as a DNA Screening Locus

The viability of using the highly polymorphic DNA locus, HUMACTBP2 (SE33), as a screening locus on heavily bloodstained evidence prior to full profile DNA testing was assessed. Genotyping at the SE33 locus was conducted on 160 Caucasians from the Lehigh Valley region of Pennsylvania. Results showed that the population conformed to Hardy-Weinberg equilibrium and that the locus had an observed heterozygosity of 0.944. Sensitivity, mixture and degradation studies were conducted. Typing results were obtained with as little as 0.0625 ng of DNA and the minor component of a two-component mixture was genotyped in a 9:1 ratio in one sample. In two degradation studies, bloodstains were applied to different substrates and exposed for 2 months and 1.5 months, respectively, of northeastern Pennsylvania weather. Genotypes were obtained on more than half of the substrates containing bloodstains stored in the ambient environment for at least one month.