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Contamination by Nonfermentative Microorganisms in M‐K and Organ Culture Media

The effect of nonfermentative microorganisms on the pH indicator phenol red used in McCarey-Kaufman (M-K) medium and Organ Culture medium was examined. Separate aliquots of M-K media and Organ Culture media were inoculated with cultures of known fermentative and nonfermentative bacteria. Phenol red accurately indicated the growth of fermentative bacteria at 37°C and 4°C by exhibiting a pH-sensitive change in color, but did not reveal nonfermentative bacterial growth. A different method of detection is necessary to identify the presence of nonfermentative bacteria in these media.