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Roles of larger conductance mechanosensitive channels (MscL) in sporulation and Act secretion in Streptomyces coelicolor

Larger conductance mechanosensitive channels (MscL) is a conserved channel existing in eukarya, bacteria and archaea. The gene SCO3190 in Streptomyces coelicolor encodes an MscL homolog of Escherichia coli. To elucidate the physiological roles of MscL in S. coelicolor (Sc‐MscL), deletion mutant was constructed. The Sc‐MscL deletion brought sporulation ahead, and had no significant influence to antibiotic synthesis in S. coelicolor. When Sc‐MscL was overexpressed in S. coelicolor, the colony of S. coelicolor was small and sleek with abundant blue pigment corresponding to actinorhodin (Act). Furthermore, abundant blue pigment displayed on hypoosmotic LB plates comparing to hyperosmotic R5 plates. These results suggested that Sc‐MscL was involved into Act secretion and sporulation relating to osmotic stress response in S. coelicolor. Moreover, Sc‐MscL showed possible functional pentamer by cross‐linking in vitro. It is first report about biochemical and functional characterization of MscL in S. coelicolor. (© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)