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A method to improve the reproducibility of in vivo reflectance spectroscopy

The goal of this study was to optimize measurement techniques for tissue point spectroscopy during gastrointestinal (GI) endoscopy, as part of a program to enhance and apply autofluorescence/reflectance imaging for early GI cancer detection. The effect of fiberoptic probe pressure on tissue on the measured diffuse reflectance spectra was evaluated, with both fiber-to-fiber probe geometry (standard contact probe) and imaging illumination geometry (wide field illumination and fiber collection) for the wavelength range 440-640 nm, using normal skin in vivo as a model tissue, and by taking continuous spectral measurements while the fiber is approaching the tissue. The most significant finding was a sudden change in the reflectance signal that occurs as the probe comes into contact with the tissue surface.