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[Effect of certain factors on Vibrio cholerae multiplication in fermenter].

The influence of the level of dissolved oxygen, as well as the phase of growth and the amount of inoculum introduced, on the growth of bacterial population in the process of the cultivation of cholera vibrios in the fermenter "Novopalias" has been studied. The results of this study indicate that the cultivation of cholera vibrios with the level of dissolved oxygen constituting 50% of the maximum air saturation of the broth ensures a better yield of the biomass than cultivation with the level of dissolved oxygen equal to 10% and 30%. The introduction of inoculum in an amount of 1 : 20 (inoculum/medium) does not ensure a better yield of the biomass than an amount of 1 : 100 introduced at the phase of stationary growth. Cultivation at 50% pO2 and the introduction of inoculum in an amount of 1 : 100 at the phase of stationary growth have demonstrated some technical advantages in comparison with the introduction of inoculum at the phase of exponential growth, which allows one to use this technique for the production of cholera vaccine.