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Microscopic imaging of the effects of formaldehyde fixation on tissue autofluorescence

In this work, we investigated the effect of formaldehyde-induced autofluorescence using multiphoton and confocal microscopy. Porcine aorta, brain, kidney, and ventricle were processed, sliced, and immersed in 10% neutral buffered formalin at ambient temperature for different length of time. Our preliminary results show that the inducedautofluorescence in brain and ventricle tissue increases through time, whereas for aorta and kidney it remains nearly the same.