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[Establishment and characterization of a cell line (KO-RCC-1) from a human renal cell carcinoma].

A new cell line, KO-RCC-1, has been established from a primary renal cell carcinoma of a Japanese woman. The cells have been maintained for 43 months and over 130 in vitro passages. The cells are epithelioid and exhibit multilayering. Electron microscopic study showed large nuclei with prominent nucleolei, abundant glycogen particles and microvilli. The doubling time was approximately 38 hr and the cells formed colonies in soft agar with an average plating efficiency of 10. 4%. Chromosome number of the cell line was aneuploidy. Inoculation of the cultured cells into nude mice caused tumors at the site of inoculation. Histology of the nude mice tumor resembled the original tumor.