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Relationship Between Single-Nucleotide Polymorphisms of Tumor Necrosis Factor Alpha, Interleukin-10, Factor II and Factor V with Risk of Inhibitor Development in Patients with Severe Hemophilia A.

BACKGROUND
About one-fourth of patients with hemophilia A (HA) develop alloantibodies against factor (F) VIII, as the main treatment challenge. Here, we assessed the relationship between interleukin-10 (IL-10), tumor necrosis factor alpha (TNF-α) and FII and FV polymorphisms and risk of inhibitor formation in patients with severe HA.


METHODS
We divided 39 patients with severe HA in two groups of cases (n: 19) and control (n: 20). Genotyping was performed by multiplex amplification tetra arms refractory mutation system-polymerase chain reaction (ARMS-PCR) and PCR-restriction fragment-length polymorphism (PCR-RFLP).


RESULTS
TNFα rs1800629 G > A polymorphism decreased the risk of inhibitor development in codominant and dominant inheritance pattern. Moreover, TNFα rs1800629 A allele, decrease the risk of inhibitor formation, while IL10 rs1800896 A > G, FV rs6025 G > A, and FII rs1799963 G > A polymorphisms were not associated with risk of inhibitor development.


CONCLUSION
It seems that TNFα rs1800629 G > A polymorphism decreased the risk of inhibitor formation in Iranian patients with HA.