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Effect of progesterone on the MGMT gene expression in MCF7, HEp-2 and 293 cells

Aims: to investigate the steroid hormone progesterone effect on the human MGMT gene expression at the mRNA and protein levels in cell lines with different expression patterns of the nuclear progesterone receptors and membrane receptor PGRMC1. Methods: cell culture, RNA / protein isolation, cDNA synthesis, real-time polymerase chain reaction, Western blot analysis. Results: We observe the MGMT gene upregulation by progesterone at both mRNA and protein levels. Conclusions the effect of progesterone on MGMT expression is more complex than direct regulation through the classical nuclear receptor.Keywords: O6-methylguanine-DNA methyltransferase (MGMT), progesterone, nuclear progesterone receptors (nPR), progesterone receptor membrane component 1 (PGRMC1), gene expression regulation.