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Sperm plasma membrane integrity in fertile and infertile men

Summary. Sperm plasma membrane characteristics were analysed by a combined method, the HOS‐eosine test (HOS‐E test), that consists of the hypoosmotic swelling test (HOS test), and the eosine‐Y staining. Semen samples were categorized into four groups (Normo‐, Oligo‐, Astheno‐, and Oligoasthenozoospermic) and subjected to the standard analysis (spermiogram), HOS test, eosine‐nigrosine test (reflecting sperm viability), and HOS‐E test.