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[Plasma Free mtDNA and Expression of TLR-9/MAPK in Brain Tissues of Rats with Subarachnoid Hemorrhage].

OBJECTIVES
To determine the plasma level of mitochondrial DNA (mtDNA) and the expression of Toll like receptor-9/mitogen-activated protein kinase (TLR-9/MAPK) in the brain tissues of rats with subarachnoid hemorrhage (SAH).


METHODS
Sprague-Dawley rats were used to establish SAH models with a modified method of endovascular perforation. Twenty four hours after the establishment of the models, the plasma level of mtDNA in the rats was detected by real-time PCR; the expressions of TLR-9 and P38 MAPK in the brain tissues were detected by reverse transcription PCR (RT-PCR) and immunohistochemical staining. Differences between the SAH (n =20) and sham-surgical (n =20) groups were compared.


RESULTS
Rats in the SAH group had higher levels of plasma mtNDA and expressions of TLR-9 and P38 MAPK in brain tissues than those in the sham-surgical group (P <0.05).


CONCLUSION
Plasma free mtDNA and expressions of TLR-9/MAPK increase simultaneously in rats with SAH. mtDNA may induce system and local inflammation reactions in brain.