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[Study on extraction methods of volatile oil from Mosla soochowensis].

OBJECTIVE
To study the different extraction methods of the volatile oil from Mosla soochowensis Matsuda and to determine the optimum extraction method.


METHODS
The yields and the chemical constituents of volatile oil were used to compare the different extraction methods including SFE-CO2, steam distillation and organic solvent (petroleum ether) extraction.


RESULTS
The yield of volatile oil by means of SFE-CO2 was 3.46%, which was higher than others. The quality of the oil by means of SFE-CO2 and steam distillation was better. Based on the result of GC-MS, the constituents of volatile oil were similar. It was mainly the methyleugenol, accounted for about 42%. The second was monocyclic terpene, such as bornene, dihydrocarvon, carvacrol, accounted for about 27%. The third was sesqutierpenoids, such as nerolidene etc, accounted for about 19%.


CONCLUSION
SFEE-CO2 is the optimum extraction method of the volatile oil from Mosla soochowensis Matsuda.