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Water permeability changes of the cell membrane as a function of time

Water loss and microscopic water migration inside tissues or cells are major causes of vegetable deterioration and wilting during the postharvest period. Water loss and water migration caused by respiration and transpiration are influenced by the condition of the cell membrane or other biomembranes. Therefore, for the studying on postharvest technology, it is essential to understand the changes in the properties of biomembranes of the agricultural products during the storage. The change in cell membrane water permeability coefficient (L p ) and osmotic tolerance of barley cotyledon protoplast was measured and the change in dynamic state of water in the cotyledons was estimated by 1 H-NMR during the storage. The barley cotyledons were cut from the plants and stored in test tubes at 20°C in dark. The storage periods were 0, 24, 48 and 72 hours. After the storage, protoplasts were separated from the cotyledons and the Lp of the cell membranes were measured by Two Laminar Flow (TLF) Method.[...]