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Evaluation of five hepatitis delta virus marker assays for detection of antigen and antibody

Five commercially available assays for hepatitis delta (HD) virus markers were compared for sensitivity, specificity, and reproducibility: three assays for antibody (anti-HD), provided by Diagnostics Pasteur, Organon Teknika, and Abbott Laboratories, and two assays for antigen (HD Ag), from Pasteur and Organon Teknika. The assay from Organon Teknika is the less sensitive assay for anti-HD detection. Although the sensitivities of the Pasteur and Abbott assays for anti-HD detection are similar, the use of Abbott reagents is hampered by the lack of specificity when HD Ag is present. The greater sensitivity for HD Ag detection is obtained with Organon assay.