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The dual role of phytochemicals on SARS-CoV-2 inhibition by targeting host and viral proteins

Background The severe acute respiratory syndrome-2019 has affected more than 190 million people around the world and caused severe crises throughout the globe. Due to rapid mutation in the viral genome, its became important to simultaneously improvise the host immunity while targeting viral proteins to reduce the severity of infection. Aim The current computational work focuses on multi-level rigorous screening of 47 medicinal plant-based phytochemicals for discovering effective phytochemical inhibitors against the host and viral targets. Experimental procedure A total of 586 phytochemicals were analyzed in detail based on their drug-likeness, pharmacological properties, and structure-based activity against the viral proteins (Spike glycoprotein, Papain-like protease, and Main protease) and host proteins (ACE2, Importin-subunit α-5, and β-1). Phytochemicals showing higher binding affinity with the dual capacity to target both the categories of proteins were further analyzed by profiling of their chemical reactivity using Density-Functional Theory (DFT) based quantum chemical methods. Finally, detailed molecular dynamics simulations were performed to analyze the interactions of the complexes. Results and conclusion The results revealed that the selected phytochemicals from Andrographis paniculata, Aconitum heterophyllum, Costus speciosus and Inula racemosa may have the capacity to act with prominent affinity towards the host and viral proteins. Therefore, the combination of active phytochemicals of these plants may prove to be more beneficial and can be used for developing the potential phytotherapeutic intervention.


Introduction
A novel corona virus (SARS-CoV-2) has been associated with infectious respiratory disease, hastily spreading in the human population. 1,2 Up to April 2021, this disease has spread in more than 219 countries proving to be a global pandemic. 3 Alarmingly, the number of infected patients is rising day by day. At an early stage of infection, a person is associated with a cough, fever, shortness of breath, and headache which further leads to severe pneumonia. The epidemiological studies have revealed that apart from the age factor, an individual's immunity is also severely compromised due to this virus. 4 5,6 The structural analysis of SARS family reveals that SARS-CoV-2 is a single-stranded RNA virus having genes for Spike (S), Envelope (E), Membrane (M), Nucleocapsid (N) and Non-structural proteins (NSPs). 7e10 Once the virus enters the nucleus, replication and packaging of its genetic material begins by using its non-structural proteins. This viral cycle causes respiratory tract infection which eventually leads to respiratory failure, cardiac arrest, and other multi-disorders.
Various therapeutic developments are in progress to discover an effective vaccine and drugs, which can directly target the viral proteins. In this direction, traditional medicines are effective to respond against various viral pathogenesis as well as in the host immunity modulation. Considering the antiviral properties of these medicinal plants, researchers are focusing on various phytochemicals that can act as potent drug components in the treatment of SARS-CoV-2 infection. Currently, more than 100 clinical trials based on medicinal plants are in progress (https://www.clinicaltrials.gov/ ). A recent work by Maurya et al. revealed a significant binding of Curcumin and Nimbin with Spike protein of SARS-CoV-2 and humane ACE2 receptor. 11 The beneficial role of Nictoflorine, Aloenine, and Berberine for the inhibition of protease enzyme was also reported. 12 Steroidal phytochemicals of Withania somnifera and triterpenoids of Azadirachta indica can inhibit the SARS-CoV-2 proteins. 13,14 These overhead studies are concentrated toward the understanding of the plant-based bioactive compounds to decrease the virulence of SARS-CoV-2. Interestingly, these studies also suggest that medicinal phytochemicals can target both virus-basedproteins and host-based receptors and thus directly impact the viral cycle which is dependent on the host proteins.
Among the viral targets, the most common is the Spike protein which is mainly responsible for the entry of corona virus into the host cell by interacting with the ACE2 receptor. 15,16 Spike protein is made up of two subunits (S1 and S2) where a sequence of S1 subunit is the most variable part of the SARS-CoV-2 genome. The emerging variants of concerns such as delta (B1.617.2) and kappa (B1.617.1) are known for their high infectivity rate. It is reported that several double/multiple mutants in the spike protein of SARS-CoV-2 are enhancing the viral host interactions. Sequence analysis defines that variant B1.617.2 and B1.617.1 have two mutations in the Receptor-Binding Domain (RDB) of the spike protein. The key mutation for B1.617.2 (delta variant) is L452R, T478K and L452R, E484Q for B1.617.1 (kappa variants), respectively. These variants are now getting huge consideration because of their antibody neutralizing tendency. 17 Inside the host cell, the viral genome shows the expression of PL pro and 3CL pro /M pro that are responsible for the formation of various non-structural proteins and further utilize the nuclear importins to enter the nucleus of the host cell. 15 ACE2, Importin subunit a-5, and Importin subunit b-1 are the few major host proteins that are involved in the viral infection. Drugs like Ivermectin act by targeting the Importin a/b receptor of the host to weaken the viral cycle. 18,19 It is also reported that drugs acting as allosteric modulators can decrease the strength of spike and ACE2 complex that will further help in the reduction of viral incubations. 20 In this context, identifying medicinal bioactive compounds that can selectively target both virus-based-proteins and host-basedreceptors simultaneously and might have the potential to alleviate the effect of SARS-CoV-2 infection. 21,22 Therefore, in this investigation, we have selected 586 phytochemicals from 47 medicinal plants which have immune boosting properties and exhibit antiviral activity for different viral infections. The aim of this study was to identify active compounds against the spike glycoprotein, PL pro and M pro receptors of virus and ACE2, Importin subunit a-5 and Importin subunit b-1 of the host machinery in a cooperative manner. 23,24 Different properties of the phytochemicals such as drug likeness, pharmacokinetic parameters are calculated. Their target binding efficiency was also calculated and compared to different drugs reported for the chosen protein targets. Control drugs such as Arbidol (spike), Disulfiram (PL pro ), Lopinavir (M pro ) and Hydroxychloroquine (ACE2), Ivermectin (importin-alpha-5 and importin-beta-1) were considered to systematically compare the reactivity of the resulting plant based bioactive compounds. 25 Finally, DFT based reactivity properties were calculated and molecular dynamics simulations were also performed to understand the protein-ligand interaction.

Curation of phytochemicals
We have created a library of 586 bioactive phytochemicals having antiviral properties from 47 medicinal plants from the literature (Supplementary Table 1). 21,23,24,26e41 The 3D .sdf structures of all the selected phytochemicals were obtained from the PubChem database (https://pubchem.ncbi.nlm.nih.gov/).

Drug-likeness properties and ADME/toxicity prediction
All the selected phytochemicals were screened for their druglikeness based on Lipinski, Ghose, Veber, Egan & Muegge rules by using SwissADME (http://www.swissadme.ch/). Further, those phytochemicals which fulfilled the criteria of drug-likeness were checked for their Absorption, Distribution, Metabolism, Excretion and Toxicity (ADMET) properties. The pharmacokinetics and pharmacodynamics properties evaluation were done with the help of admetSAR (http://lmmd.ecust.edu.cn/admetsar1/predict/). After the ADMET analysis, the phytochemicals showing efficient pharmacokinetic parameters were selected for the molecular interaction studies with SARS-COV-2 viral and the host targets.

Protein and ligand preparation
The key therapeutic targets of virus, as well as the host proteins, were subjected to the screening of phytochemicals. The threedimensional crystal structure of the selected viral targets for SARS-CoV-2 such as spike glycoprotein (PDB ID: 6LZG), PL pro (PDB ID: 6W9C), M pro (PDB ID: 6LU7), and the host protein targets that includes ACE2 (PDB ID: 6M0J), Importin subunit a-5 (PDB ID: 2JDQ), and Importin subunit b-1 (PDB ID: 1F59) were chosen from protein data bank (https://www.rcsb.org/) and are presented in Supplementary Fig. 1. Files of the target protein retrieved from the PDB databank were prepared by removing water molecules, heteroatoms, and extra chains. We also included new SARS-CoV-2 variants of ceoncern such as B1.617.1 (Kappa) and B.1.617.2 (Delta) of B.1.617 lineage to address the interaction potential of phytochemicals. These variants of Spike RBD were studied, starting from Top performing phytochemicals from the property calculation were selected to evaluate their interaction with the mentioned protein targets. For this purpose, a systematic comparison was performed with the known drugs namely Arbidol for spike, Disulfiram for PL pro , Lopinavir for M pro and in case of host protein Hydroxychloroquine for ACE2, Ivermectin for Importin-alpha-5 and Importin-beta-1 acting as a control for the individual targets. 19,42e50 All the chemical structures were obtained from the PubChem database. Further, chemical structures including controls and phytochemicals were converted into .pdb 3D conformation and prepared for molecular docking studies by adding hydrogen atoms through UCSF Chimera.

Molecular docking studies
The molecular interaction studies were performed by using AutoDock 4.2. 51 All the structural proteins targets were set rigid and the ligands were kept flexible. To adopt more than one ligand conformation, 90 Â 90 Â 90x grid box was prepared around the active site of each target protein. The Lamarckian Genetic Algorithm (LGA) was selected as the search algorithm. All the other parameters and setting of docking were kept as standard. Further, for the generation of grid parameter file and docking parameter file, Cygwin was used. The docked conformations of each ligand were selected on the basis of binding energy and the top-ranked conformations. The docked conformations of complexes were visualized with the help of Discovery Studio Visualizer. 52

Quantum chemical calculations
Phytochemicals in association with their control compounds were optimized to obtain most favorable binding energy by using the Gaussian 16 suite to understand their stability and reactivity nature. 53 For the ground state geometry optimization (in gas phase) DFT based Becke's three parameter exchange function (B3) with Lee-Yang-Parr hybrid density functional (LYP) with 6e31G (d, p) basis set were used. 54,55 Further Koopman's theorem was applied on optimized geometries for the calculation of highest occupied molecular orbital (HOMO), lowest unoccupied molecular orbital (LUMO) and HOMO-LUMO band gap. 56,57 Simultaneously, different thermodynamic properties, such as global reactivity parameters (as chemical potential and chemical hardness), heat capacity and entropy were also calculated. 58

Molecular dynamics (MD) simulations
MD simulations of the strongest protein-ligand complexes as compared to the control drug were performed to understand the conformational changes and dynamic properties of the interactions. A total of eight systems were selected and simulated using GROMACS 2015 (Groningen Machine for Chemical Simulations) with GROMOS96 54a7 force field. 59 ATB server was used for the generation of ligand parameters associated with the chosen force field. The protein-ligand complexes were solvated in dodecahedron boxes by single point charge (SPC) water molecules and the system was neutralized by adding counter ions. 59,60 Further, all the eight systems were subjected to energy minimization by running the steepest descent minimization integrator at 50,000 steps with <10 kJ/mol force convergence. Subsequently, equilibrations of all complexes were performed by using NVT (canonical) and NPT (isothermal-isobaric) ensembles at temperature 300 K. Thereafter, the systems were simulated for the 50 ns and were analyzed by using different parameters such as Root Mean Square Deviation (RMSD), Root Mean Square Fluctuation (RMSF), and a number of hydrogen bonds interactions of the protein-ligand complexes.

Curation, preparation and screening of phytochemicals
Among the evaluated 586 phytochemicals, 161 were found to follow the drug-likeness criteria. These phytochemicals had a molecular weight below five hundred, LogP was less than five, hydrogen bond acceptors count was below ten and hydrogen bond donors counts were under five. Further, these phytochemicals were also screened for the pharmacokinetic properties such as Aqueous Solubility, Blood-Brain Barrier (BBB) penetration, Human Intestinal Absorption (HIA), Cytochrome enzyme inhibitor, Mutagenicity (AMES-Test) and Carcinogenicity. The results represented that, 24 phytochemicals (Table 1) were showed favorable activity in these parameters. The permissible limit criteria of pharmacokinetic parameters for all 24 phytochemicals showed that their solubility was in the range between À6.5 to 0.5, with acceptable BBB limits found to be in between À3.0 to 1.2, and HIA was more than 30% with low Cytochrome enzyme inhibition (Supplementary Table 2).

Protein modeling and validation
From literature, we found that the Kappa variant mutations (in the residues L452R (452, leucine-to-arginine), E484Q (484, glutamic acid-to-glutamine)) and the Delta variant mutations (at L452R (452, leucine-to-arginine) & T478K (478 is threonine-to-lysine)). These changes are incorporated to the Spike RBD FASTA sequence and used for mutant RBD homology modeling using the SWISS-MODEL. Validation was performed by Ramachandran plot analysis of modeled Kappa variant Spike RBD. PROCHECK shows that 89.3% of residues were in favoured regions, 10.1% residues in additional allowed regions and 0.6% in disallowed regions with 99.44% final protein quality factor by ERRAT plot analysis. For the modeled Delta variant, the Ramachandran plot shows 89.3% of residues in favoured regions, 10.1% residues in additional allowed regions, 0.6% residues were in disallowed regions with a 99.43% quality factor (Supplementary Fig. 7). According to validation analysis both the modeled protein is of good quality and having less error value for further molecular screening studies.

Molecular docking studies
To find out the most potent phytochemical from amongst the selected 24 phytochemicals, with the highest inhibition capacity, molecular interactions studies were performed. These phytochemicals were further categorized based on their binding energies, hydrogen bonds, hydrophobic interaction and interactive amino acid residues.
The binding energies of all the selected phytochemicals were compared with their chosen control drugs (Supplementary Table 3, Supplementary Figs. 2 and 3). Additionally, the binding energies of all 24 phytochemicals were plotted (ranging between À2.0 kcal/mol to À8.0 kcal/mol) against the host and viral targets (Fig. 1). The plot is depicting that the 14-deoxy-14, 15-didehydroandrographolide shows comparatively high binding energy with all targets except Importin subunits among the chosen chemicals.
Potential inhibitors for the host targets: In the category of host-based targets (ACE2, Importin subunit a-5, and Importin subunit b-1), the binding energies of the screened phytochemicals were compared with those of the respective control drugs as Hydroxychloroquine for ACE2, Ivermectin for Importin subunit a-5 and Importin subunit b-1. All the 24 phytochemicals with ACE2 target had binding energy in the range of À4.42 to À8.46 (kcal/mol) and that for the control compound, it was À5.19 (kcal/mol) (Supplementary Table 4). Hetisinone (CID_101930090) from Aconitum heterophyllum showed the highest binding energy (8.46 (kcal/mol)) among all selected phytochemicals. In case of Importin subunit a-5, and Importin subunit b-1 the binding energies for phytochemicals ranged between À7.22 to À2.0 (kcal/mol) and À7.95 to À4.83 (kcal/mol) for their respective targets Table 1 Structure of phytochemicals following the drug likeness properties obtained after screening of compounds from the 47 medicinal plants.  Tables 5 and 6). Control compound (Ivermectin) was found to be binding more favorably with the Importin subunit a-5 and Importin subunit b-1 as compared to that of the chosen phytochemical for these targets (Fig. 2). Potential inhibitors for SARS-CoV-2 (Viral) targets: In the case of viral proteins (spike glycoprotein, PL pro and M pro ) all the selected 24 phytochemicals were also screened to check their interactions by molecular docking. We have also performed the screening of top interacting phytochemicals with the Spike RBD of B1.617.1 (Kappa) and B.1.617.2 (Delta) of B.1.617 lineage. The results showed that the binding energy of the phytochemicals with spike proteins was in the range of À4.65 to À7.76 (kcal/mol) and for the control compound (Arbidol) binding energy with spike protein was À5.9 (kcal/ mol) (Supplementary Table 7 & Fig. 2). 14-deoxy-11, 12didehydroandrographolide (CID_15708351) from Andrographis paniculata was showing maximum binding score À7.76 (kcal/ mol), À7.4 with (kcal/mol) and À7.09 (kcal/mol) with Spike protein as well as with both mutants B1.617.1 (Kappa) and B.1.617.2 (Delta), respectively ( Supplementary Table 11 & Fig. 3)). For PLpro and Mpro receptors, the binding energies of the phytochemicals ranged between À5.06 to À8.20 (kcal/mol) and À4.17 to À7.40 (kcal/mol) respectively. When compared with their respected control compounds Disulfiram (PLpro), and Lopinavir (Mpro), the obtained energy was found to less favorable as the energies were À3.11 (kcal/ mol) and À5.21 (kcal/mol) respectively (Supplementary Tables 8  and 9). The phytochemical Germacranolide (CID_101616641) from Inula racemosa and Costunolide (CID_5281437) from plant Costus speciosus were found to be showed the highest binding score À8.20 and À7.40 kcal/mol for PLpro and Mpro, respectively (Fig. 2 (e, f)).

Analysis of frontier molecular orbital and reactivity parameters
All the screened and shortlisted phytochemicals were next optimized at their minimum energy state with zero imaginary frequency (Fig. 3) and their molecular properties were computed. All the optimized compounds were found to be stable and nonplanar (except disulfiram).
Highest Occupied Molecular Orbital (HOMO), and Lowest Unoccupied Molecular Orbital (LUMO) represent the frontier molecular orbitals (FMOs) of chemicals. The energy of HOMO and LUMO are one of the most significant aspects to describe the chemical/ bioactivities of drug components. Higher the value of E HOMO, higher will be its electron donating ability. 61 For all selected phytochemicals the energy of HOMO was higher in comparison to that of their respective control compounds. The energy of HOMO for phytochemicals and the control were obtained to lie in between À6.56 eV and À6.02 eV and À5.76 eV to À5.39 respectively. However, the variation in E LUMO reflected the presence of electron giving or extracting groups in the structure (Supplementary Fig. 4).
The chemical reactivity parameters such as chemical hardness (h), and electronic chemical potential (m), were also calculated for all the phytochemicals and known drug compounds. 56 Chemical hardness is a parameter which defines the stability/reactivity of a particular chemicals and lower heat capacity with a positive entropy, is typically associated with hydrophobic interactions and conformational changes of ligand during binding. 58 The values of chemical hardness for phytochemicals ranged between 2.43 eV and 3.09 eV and were found to be higher than those of their respective drug compound. The value of the heat capacity (between 64.58 and 92.91) and entropy (127.69e161.08) for phytochemicals was quite low. Therefore, higher the chemical hardness with lower entropy parameter shows the high stability with lower randomness for all phytochemicals than their respective drug compounds 62 (Supplementary Table 10 and Fig. 4).

Molecular dynamics simulation analysis
The MD simulation helped to understand the stability and conformational variation in the protein-ligand complexes (49). Based upon the molecular docking interaction trends, among the host proteins, ligands interacted most favorably with ACE2 receptor. Thus, in the host category, simulation undertaking Hetisinone phytochemical was performed with ACE2 receptor and compared it with control drug Hydroxychloroquine (HCQ). The obtained RMSD profile (Fig. 5 (a)) showed that in case of Hetisinone, the stability of the protein backbone was better and remained within the range of 0.2 nme0.3 nm. However, in the case of HCQ, larger fluctuations were observed around 30 ns, and which continued till 50 ns Thus, the backbone initially showed fluctuation, however, attained equilibrium after 10ns in both the cases. To check the flexibility of interacting residues RMSF was calculated. It was observed that the rate of amino acid fluctuation for Hetisinone was higher in comparison to that of HCQ (Fig. 5(b)). The RMSF value ranged in between 0.2 and 0.8 nm for Hetisinone and for HCQ it ranged between 0.2 and 0.6 nm. The increased value of RMSF shows higher flexibility of the complexes at the time of simulations. Finally, the analysis of intermolecular hydrogen bonds (H-bonds) was performed to check the binding stability of the ligands with their targets (Fig. 5 (c)). Following this, Phytochemical Hetisinone formed 6 H-bonds whereas, HCQ formed 4 H-bonds. Based upon these observations it can be concluded that the hydrogen bond interaction between Hetisinone and ACE2 represented a strong  interaction in comparison with control drug molecule. HCQ disrupts the viral-host interaction by binding to an allosteric region of the ACE2 receptor. This allosteric interaction can inhibit the viral invasion inside the human cell. Although Hetisinone exhibited nonspecific binding on to the ACE2 receptor, it has the potential to demonstrate better activity specific to SARS-CoV-2.
During the SARS-CoV-2 infection, Spike glycoprotein and papain-like proteases (Pl pro and M pro ) are the essential proteins for the processing and maturation of viral genome and due to this, these proteins are important therapeutic targets. Phytochemicals like 14-deoxy-11, 12-didehydroandrographolide, Germacranolide and Lopinavir were selected for detailed MD analysis to understand the binding mode with Spike, PL pro, and M pro . For Spike protein, as shown in Fig. 5 (d) the RMSD of the backbone revealed that in presence of 14-deoxy-14, 15didehydroandrographolide fluctuation in protein continued till 35 ns and attained stability, which remained in the range of 0.2 nme0.3 nm whereas with control Arbidol the RMSD reflected an increment starting from 0.2 nm to 0.45 nm.
The range of fluctuation (RMSF) in Spike protein and control is between 0.3 and 0.8 nm while with phytochemical 14-deoxy-14, 15-didehydroandrographolide it is between 0.3 and 0.6 nm (Fig. 5  (e)). 14-deoxy-11, 12-didehydroandrographolide also formed 3 stable H-bonds with Spike glycoprotein at 50 ns (Fig. 5(f)). It is known that the Arbidol has the capability to target the Spike glycoprotein which may help in blocking mechanism of the trimerization of SARS-CoV-2 spike glycoprotein, 63 the observed interaction of 14-deoxy-14, 15-didehydroandrographolide with Spike glycoprotein can possibly lead to inhibition of trimerization of the Spike protein as the binding pattern was found to be similar in both the cases.
Further, for PL pro, RMSD plot of phytochemical Costunolide, was in the range of 0.2 nme0.4 nm and the value of RMSF was between 0.4 and 0.7 nm (Supplementary Fig. 5 (a, b)). For control molecule RMSD ranges between 0.2 and 0.3 nm and RMSF was in the range of 0.4e0.8 nm. The comparative graph of RMSD for control and phytochemical with PL pro is depicts that the Costunolide is having slightly higher fluctuation with Disulfiram.
The observed range was better than that of the control molecule Disulfiram. Finally, H-bond calculation revealed that at 50 ns, 3 stable H-bonds were found between Pl pro and Costunolide whereas no H-bond was observed with Disulfiram ( Supplementary Fig. 5 (c)). Since 1951, Disulfiram is used in the treatment of alcohol aversion and is also a competitive inhibitor for SARS-CoV Plpro. 64 With another papain-like protease M pro phytochemical Germacranolide showed the most potential interaction during molecular docking studies in comparison to that of its control drug molecule (Lopinavir).
The comparative molecular dynamics analysis of Germacranolide with M pro protein revealed fluctuations in RMSD value which lasted till 15 ns and further remained stable until 45 ns On the other hand, Lopinavir showed better stability after crossing 35 ns (Supplementary Fig. 6 (a)). We also found almost a similar RMSF peak 0.2e1 nm in case of both phytochemicals as well as in the control molecule with M pro (Supplementary Fig. 6 (b)). The Hbond plot showed that Germacranolide created 4 stable H-bonds at 50 ns which were quite higher than that of Lopinavir ( Supplementary Fig. 6 (c)). During the SARS-CoV-2 treatment, Lopinavir can inhibit SARS-CoV-2 proteases in combination with other drugs by binding to the active site of M pro protein. 65 Considering the results, it can thus be concluded that Germacranolide can target M pro a more effective manner based upon its interaction dynamics.
During the SARS-CoV-2 infection, the host protein defense mechanism is activated to suppress the viral invaders and at the same time, SARS-CoV-2 captivates the host cellular mechanism for their replication and protein translation mechanism. This viral attack further leads to tissue damage and hyper inflammation in the host immune system. In this current investigation, we developed a comprehensive approach to reduce COVID-19 infection by targeting conserved viral proteins that are responsible for the entry and replication of SARS-CoV-2 as well as by inhibiting the activity of those host protein which facilitated viral replication inside the body. This strategy advantageous while considering the range of mutation and the effect of SARS-CoV-2 on the host organ system. This study reveals that, for the treatment of COVID-19, combination of active phytochemicals of Andrographis paniculata, Aconitum heterophyllum, Costus speciosus and Inula racemosa may prove to be more beneficial and can be used for developing the potential phytotherapeutic intervention.
Multi-scale computational screening of the selected phytochemicals indicate that these compounds can act as cooperative and multi-target allosteric regulators and offer a unique category for drug development against COVID-19 infection. The approach of finding the appropriate phytochemicals targeting virus-host interaction and the host proteins together might be a new regimen for SARS-CoV-2 treatment.

Conclusion
SARS-CoV-2 pandemic has become a major challenge to the global health system with an increasing number of infections all over the world. Viral proteins are the key factors of SARS-CoV-2 virulence which allows virus to replicate and affect the host defence mechanisms. So, it is required to decipher the potential therapeutics including active phytochemicals for the treatment of COVID-19 infection. Herein, we screened 586 potential antiviral phytochemicals for the drug-likeness, efficient pharmacokinetics analysis, and excellent binding energy parameters against key viral and the host components. Additionally, our study has also included on evaluating phytochemicals potency against the spike RBD of novel SARS-CoV-2 variants Kappa (B1.617.1) and Delta (B.1.617.2). Overall, our systematic analysis found that 14-deoxy-11,12-didehydroandrographolide, Costunolide, Germacranolide and Hetisinone can act as probable inhibitory phytochemicals that can help in reducing the burden of SARS-CoV-2 infection by acting upon the host system as well as viral targets. This work adds strong support to phytochemical medication as a cooperative multi-target treatment and recommended further in-vitro and in-vivo validation of these chemicals to convert them as clinical drugs for SARS-CoV-2.

Consent for publication
All the authors have read and approved the manuscript in all respects for publication.

Declaration of competing interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.