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[Application of fingerprint of active part on extraction techniques for Vernonia anthelmintica].

OBJECTIVE
To probe into feasibility of extraction techniques for Vernonia anthelmintica using HPLC fingerprint of active part as model.


METHODS
The extraction techniques were studied by multi-index test experiment formula evaluation and orthogonal design, which took change of peak area in HPLC fingerprint of active part for Vernonia anthelmintica as indices.


RESULTS
The optimum extracting procedure was as follows: using 10 times volume of water for each time, soaking one hour and then boiling for three times sustaining one hour each.


CONCLUSION
It is scientific, simple and applicable.