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IN VITRO ANTIFUNGAL ACTIVITY AND CYTOTOXICITY SCREENING OF DRY CRUDE EXTRACTS FROM BRAZILIAN AMAZONIA PLANTS

Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant 
species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from 
Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were 
evaluated. 
Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were 
established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent 
evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) 
10231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and 
Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy 
(SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts. 
Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis. 
Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies 
are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for 
drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis.