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IR thermocycler for centrifugal microfluidic platform with direct on-disk wireless temperature measurement system

We present an infrared (IR) thermocycler with closed loop temperature control for performing fast polymerase chain reactions (PCR) in centrifugal microfluidics [1]. It consists of an IR ring heater and an on-disk wireless temperature sensor module with a resolution of 0.1 K [2]. The closed loop system enables to precisely control the temperature of the reagents even at varying conditions e.g. manufacturing tolerances of the polymer film disks [3], different locations of the cavities, ambient temperature changes. Due to the direct heating of the reagents by IR absorption we achieve fast average heating gradients of up to 4 K/s. Average cooling gradients so far are limited to 1.3 K/s. Our system is superior in terms of energy efficiency, temperature accuracy and overall reproducibility and robustness.