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Seminal Fluid Androgen Binding Protein

The following study was undertaken to determine the presence of an androgen binding protein (ABP) in the ejaculate. Seminal fluid was obtained from ten fertile subjects. Sperm counts ranged from 20 times 106/cc to 80 times 106/cc. Each sample was preincubated with tritiated dihydrotestosterone (DHT) and examined by polyacrylamide gel electrophoresis (PAGE). Second, after preincubation of the samples with tritiated DHT, the samples were mixed with Concanavalin‐A bound to sepharose‐D (C‐S) and the supernate separated and counted. Finally, saturation analysis of the samples using dextran‐coated charcoal for separation was performed and Scatchard analysis was used to determine the concentration of ABP present.