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Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate

ABSTRACT Recently, we reported the cloning and characterization of short isoform of the icilin-activated cold receptor TRPM8 channel in keratinocytes, dubbed eTRPM8. We demonstrated that eTRPM8 via fine tuning of the endoplasmic reticulum (ER) – mitochondria Ca2+ shuttling regulates mitochondrial ATP and superoxide (O2•-) production and, thereby, mediates control of epidermal homeostasis by mild cold. Here, we provide additional information explaining why eTRPM8 suppression and TRPM8 stimulation both inhibit keratinocyte growth. We also demonstrate that stimulation of eTRPM8 with icilin may give rise to sustained oscillatory responses. Furthermore, we show that ATP-induced cytosolic and mitochondrial Ca2+ responses are attenuated by eTRPM8 suppression. This suggests positive interplay between eTRPM8 and purinergic signaling pathways, what may serve to facilitate the ER-mitochondria Ca2+ shuttling. Finally, we demonstrate that cold (25°C) induces eTRPM8-dependent superoxide-mediated necrosis of keratinocytes. Altogether, these results are in line with our model of eTRPM8-mediated cold-dependent balance between keratinocyte proliferation and differentiation.


Introduction
Under influence of ambient factors, the skin epidermis undergoes continuous regeneration through so-called epidermal homeostasis (EH): the fine-tuning of the balance between proliferation, directional migration, differentiation and death of keratinocytes. Deviation of the ambient temperature is one of the most important stimuli which constantly affect mammals' skin. Changes in the ambient temperature are perceived by thermoreceptors, the ion channels which belong to the transient receptor potential (TRP) super family (for a review see ref. 1 ). Keratinocytes may directly transduce thermal stimuli via warmth-activated TRP channels, TRPV3 (with an apparent threshold of about 32-39 C) and TRPV4 (27-34 C). [2][3][4] Nevertheless, it is important to note that at ambient temperatures from C10 C to C30 C, the unprotected human skin temperature settles at mean steady-state values within the range of C24 C to C33 C, respectively, 5 which is within the functional range of TRPM8 cold sensitivity. 6,7 Yet, the molecular identification of the cold transducer which translates cooling into downstream signaling events in keratinocytes was lacking.
In a recent investigation, 8 we reported the cloning and characterization of a new 4-trasmembranedomain epidermal isoform of the TRPM8 cold receptor-channel, dubbed eTRPM8. This channel is localized and functions in the keratinocyte endoplasmic reticulum (ER) membrane but not in the plasmalemma. Activation of eTRPM8 within ER-mitochondria contact sites facilitates mitochondrial Ca 2C uptake, thus, affecting mitochondrial Ca 2C  Also, due to space limitations, some interesting observations were bypassed in our recent publication. 8 Here we provide these results to reinforce our hypothesis with additional insights. Firstly, we demonstrate that the modalities of eTRPM8 activation define the cell response toward proliferation or differentiation. While sustained stimuli slowed down cell cycle and induced differentiation, repeated transient stimuli increased proliferation rate. Secondly, we provide additional evidence for positive interplay between eTRPM8 and purinergic signaling pathways, which may serve to facilitate the ER-mitochondria Ca 2C shuttling. Namely, we demonstrate that ATP-induced Ca 2C release from the ER and concurrent mitochondrial Ca 2C uptake depend on eTRPM8 activity. Thirdly, we show that icilin, mimicking cold stimulation, induces an eTRPM8 oscillatory activity lasting at least for tens of minutes. Finally, we complement our previously reported observation, that the decrease of the ratio of proliferating to the late differentiated keratinocytes dropped after a 3-days incubation at 25 C is associated with an increase of O 2 content in an eTRPM8-dependent manner, 8

Results and discussion
Either sustained stimulation of eTRPM8 or its knockdown, both decrease cell growth In two recent studies, 8,9 we reported that cold incubation of keratinocytes impairs their growth. We also cloned and characterized a novel archetype of TRP channels in epidermis, 8 dubbed eTRPM8, and we demonstrated that it was a functional channel with 4 transmembrane domains ( Fig. 1A) instead of the 6 original in full-length TRPM8. We also demonstrated that icilin application on the shaved skin of mice deceases the proliferation index of keratinocytes in an eTRPM8-dependent manner. 8 Fig. 1B shows that this effect can be reproduced by a 3day incubation of HaCaT cell line in a medium supplemented with 20 mM icilin (renewed daily). Because Icilin is not very stable at 37 C, we have chosen a 20 mM concentration to prevent loss of effect due to degradation. eTRPM8 Knockdown prevented pulsed iciline-mediated increase in cell growth at 37 C (Fig. 1C) and thus demonstrate eTRPM8 specific effect of icilin. qPCR experiment revealed no modification in eTRPM8 expression level in HaCaT cells incubated at 31 C or in the presence of icilin (Fig. 1D). In contrast, incubation of the cells at 25 C enhanced eTRPM8 expression. This is in a line with the observation that the incubation at 25 C causes stronger reduction of the cell growth than incubation at 31 C or with icilin ( Fig. 1B). Yet, the reduction of HaCaT cell growth caused by incubation with icilin or at 31 C was observed only in the cells expressing eTRPM8.
To what extent the modalities (i.e., intensity, duration and frequency) of eTRPM8 stimulation may affect the cell fate? To address this we explored the effect of "pulsed" stimulation HaCaT cells with icilin. In these experiments, the cells were exposed to icilin for 10 min per hour 8 times a day during 3 d. In contrast to sustained 3-day stimulation, which reduces the cell growth, the pulsed stimulation increases the cell growth about 1.4 times (Fig. 1B). This demonstrates that the modality of icilin stimulus can evoke the opposite effects on cell growth. This effect is eTRPM8 specific, since pulsed icilin stimulation did not augment the growth of eTRPM8-silenced HaCaT cells ( Fig. 1C and E). Notably, sustained icilin stimulation reduces the cell growth to the same extent as eTRPM8 suppression ( Fig. 1B and C). It was previously reported that over time, the activity of fulllength TRPM8 run down in a PIP 2 -dependent manner. 10 The eTRPM8 activity also strongly depends in bioavailability of PIP 2 8 and, therefore, should be regulated in the same way. Also, enhanced Ca 2C leak from the ER caused by sustained stimulation of eTRPM8 may lead to the decrease of the ER calcium load ( Fig. 3C and E). Both the ER depletion and eTRPM8 desensitization should, over time, reduce the ER-mitochondria Ca 2C transfer. It, therefore, seems likely that either suppression of eTRPM8 or its sustained activation by icilin may have similar effect on the cell growth, even though they engage different mechanisms to control keratinocyte cell cycle. This hypothesis was supported by the results of FACS experiments (see in Methods). The proportion of the cells entering cell cycle decreased following sustained incubation with 20 mM iciline, while the population of the cells in S-phase increased (Fig. 1F). This suggests that icilin increases the number of proliferating cell while it slows down of cell cycle. Conversely to icilin treatment, the proportion of non-proliferating cells was similar between control cells and eTRPM8 KD cells, while the fraction of the cells in G2/M-phase was larger in eTRPM8 KD keratinocytes than control cells suggesting a long-lasting arrest at G2/M checkpoint. In summary, this demonstrates that the suppression and sustained stimulation of eTRPM8 engage different signaling pathways, even though the end effect on the cell growth was similar.

A positive interplay between eTRPM8 and purinergic signaling pathways promotes keratinocytes proliferation
In Bidaux et al., 8 we demonstrated correlation between thermo-dependent dynamics of [ATP] changes and the dynamics of the cell growth, but did not provide any evidence that eTRPM8-mediated proliferation of keratinocytes at 37 C may recruit ATP-linked signaling pathways. Activation of P2Y receptors (P2YRs) via InsP 3 production stimulates the ER-to-mitochondria Ca 2C shuttling via inositol trisphosphate receptors, InsP 3 Rs. 11,12 We, therefore, assessed whether eTRPM8 activity affects in any way the InsP 3 R-mediated Ca 2C shuttling facilitated by P2YR activation. We found that 30 mM suramin, the P2YR inhibitor, decreases the growth of control HaCaT cells by 2.2-fold, while it reduces the growth of eTRPM8 KD HaCaT cells only by 1.4-fold ( Fig. 2A). Furthermore, using confocal imaging of fluo-4 and rhod-2 fluorescence, we compared ATP-induced changes in [Ca 2C ] c and [Ca 2C ] m in control HaCaT cells (Fig. 2B), in HaCaT cells pre-treated with the TRPM8 inhibitor capsazepine (Fig. 2C) and in eTRPM8 KD cells (Fig. 2D). Either eTRPM8 inhibition or suppression both augmented the ATP-induced [Ca 2C ] c responses (Fig. 2E, panel a) and significantly attenuated concurrent mitochondrial Ca 2C uptake (Fig. 2E, panel b), as reported by the reduction of the [Ca 2C ] m response. This demonstrates that, at 37 C, eTRPM8 is recruited to the ER-to-mitochondria Ca 2C shuttling process engaged by purinergic signaling pathways, and is in line with observed decrease of the cell proliferation (Fig. 1C). Furthermore, the effect of capsazepine indicates that the ER-to-mitochondria Ca 2C shuttling, engaged by purinergic stimulation, is regulated by the eTRPM8 channel activity rather than by interaction of the eTRPM8 protein with InsP 3 R.
Activation of eTRPM8 induces long-lasting oscillatory Ca 2C shuttling from the ER to mitochondria and prevents the loss of Ca 2C in the mitochondrial matrix during hypothermia We reported previously that cold or icilin-mediated activation of eTRPM8 induced Ca 2C shuttling from the ER to mitochondria on the time scale of seconds. It is not clear, however, whether a single robust Ca 2C uptake into mitochondria can maintain [Ca 2C ] m in the mitochondrial matrix at sufficient level over long term. Here, we demonstrate that a sustained activity of eTRPM8 in HEK and HaCaT cells results in oscillatory Ca 2C releases from the ER of (   mitochondria over the period of at least 30 minutes. These oscillations were inhibited by capsazepine ( Fig. 3B; insets) and were never observed in HaCaT cells with mutated pore region of eTRPM8. 8 Similar cytosolic and mitochondrial Ca 2C oscillations and their role in the regulation of hepatocyte metabolism have been reported previously. 13,14 Maintenance of this oscillatory Ca 2C transfer requires (1) refilling of the ER with Ca 2C and (2) a repetitive Ca 2C extrusion from mitochondria, which may serve to prevent activation of cyclophilin D and the permeability transition pore in mitochondria (for a review see ref. 15 ). By means of FRET-FLIM of D1ER and mtD3CPV biosensors expressed in the ER and mitochondria of HaCaT cells, respectively, we measured steady-state [Ca 2C ]. We found that incubation of HaCaT at 31 C did not alter [Ca 2C ] ER (Fig. 3C) but significantly decreased [Ca 2C ] m (Fig. 3D). It should be noted that the overexpression of eTRPM8 partially attenuated the [Ca 2C ] m decrease at 31 C while but not at 25 C (Fig. 3E). This is along the line of our previous observation of correlation between eTRPM8-mediated [Ca 2C ] m elevation and an increased bioavailability of ATP at 31 C. 8 At 25 C, however, both [Ca 2C ] ER and [Ca 2C ] m are significantly reduced (Fig. 3C and D). This may reflect a reduction in the efficiency of the Ca 2C re-uptake into the ER, which, in turn, will lead to a decrease of the Ca 2C release from the ER and, hence, to attenuation of the mitochondrial Ca 2C uptake, so that Ca 2C extrusion from mitochondria will eventually prevail. Even though eTRPM8 mRNA level is increased after a 3-day incubation at 25 C (Fig. 1B) ) is a good predictive marker for necrosis. This prompts us to assess necrosis in the keratinocytes incubated for 3 d at 25 C. As it is depicted by dark bluestained cells on the images (Fig. 4) and is quantified in bar diagram plot (Fig. 5A), the fraction of necrotic cells was negligibly small in control cells incubated at 37 C and 31 C but was elevated to 12% following 3-day incubation at 25 C. Overexpression of eTRPM8 sensitizes the cells to necrosis at 31 C and dramatically augments necrosis at 25 C (35%). Interestingly, an augmentation of necrosis by eTRPM8 overexpression can be virtually completely reversed by the concomitant overexpression of the superoxide dismutase 1 (SOD1). This suggested a causal link between [O 2 -] and necrosis, which was confirmed further by correlation analysis (Fig. 5B).
In conclusion, in our recent study and in this addendum, we characterized a new isoform of the icilin-activated cold receptor TRPM8 channel, eTRPM8,  This consequently determines the keratinocyte fate (Fig. 5C).

Cell culture
HaCaT cell line was grown in Dulbecco's minimal essential media (DMEM) (Gibco) supplemented with 2% fetal calf serum (FCS) and Kanamycin (100 mg/ ml), and [Ca 2C ] was adjusted to 1.8 mM to induce differentiation, as described previously. 9 Necrosis was assessed by the mean of Trypan Blue.

Transient expression studies
Plasmid transfection was carried out with X-treme-GENE HP DNA Transfection Reagent (Roche) at the concentration of 4 mg per million cells. SiRNA transfection was performed with 50 nM of HiPerfect transfection reagent (Qiagen). siRNA and shRNA plasmids were built in pENTER vector (Invitrogen) following manufacturer's recommendations. Control shRNA targeted TRPM8 exon 7 and shRNA anti-eTRPM8 targeted exon 20. shRNA sense sequences are: CACCAtctctgagcgcactattcaGA-GAGtgaatagtgcgctcagaga and CACCAtattccgttcggtcatc-taGAGAGtagatgaccgaacggaata (Capital letters refer to the structure of the short hairpin RNA and small letters refer to the siRNA sequence). Control of silencing efficiency was achieved by immunoblotting of total proteins extracted from Hek cells, co-transfected with either shCTL C eTRPM8pcDNA4 or shM8 C eTRPM8pcDNA4.

Analysis of TRPM8 mRNA expression
Total RNA was isolated from different cell lines using Tri Reagent mix (Sigma-Aldrich). After a DNase I (Invitrogen) treatment and phenol/chloroform purification, 2 mg of total RNA was reverse transcribed into cDNA at 42 C using random hexamer primers (Applera) and MuLV reverse transcriptase (Applera).
Qualitative PCR was performed with TaqGold polymerase (Applera) and controlled on agarose gel.
Real-time quantitative PCR was performed on a Cfx C1000 system (Biorad). For each reaction, 12.5 ng of cDNA was placed in 15 ml of final reaction medium containing 7.5 ml of 2x SsoFast TM EvaGreen® Supermix (Biorad) and 200 nM primer pairs: CGGTCATCTACGAGCCCTAC and CACACA-CAGTGGCTTGGACT. The housekeeping gene Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an endogenous control to normalize variations in RNA extracts, the degree of RNA degradation and variability in RT efficiency. GAPDH prmiers were: ACCCACTCCTCCACCTTTG and CTCTTGTGCTCTTGCTGGG. To quantify the results, we used the comparative Ct method. PCR protocol consisted of: 30 s denaturation at 95 C followed by 40 cycles of [4 s at 95 C and 30 s at 60 C], and final dissociation curve to control specificity of the amplification.
Flow cytometry was conducted with CyAn TM ADP Analyzer. Cells were harvested, then suspended in 250 ml PBS / 4% BSA / 0,1% Triton X100 with Ribonuclease A (200 mg/ml) and incubated at room temperature for 15 min. After addition of 250 ml of PBS containing 30 mg/ml of propidium iodide, cells were further incubated at room temperature for 30 min to 1 hour. Data were analyzed with FlowJo software (version 8.7).

Visualization of agonist-induced changes of Ca 2C concentration in cytosol ([Ca 2C ] c ) and mitochondria ([Ca 2C ] m )
Changes of [Ca 2C ] c were imaged using the highaffinity fluorescent Ca 2C indicator fluo-4, as described previously. 16,17 Fluo-4 was loaded by 1hour incubation of the cells with 5 mM fluo-4 acetoxymethyl ester (fluo-4 AM; diluted from a stock containing 2 mM fluo-4 AM and 0.025% (w/v) pluronic F-127 in dimethyl sulphoxide). Changes of [Ca 2C ] m were imaged using fluorescent Ca 2C indicator rhod-2: 50 mg of rhod-2 AM was dissolved in 10 ml of DMSO (containing 0.025% (w/v) pluronic F-127), which was then mixed with 4 ml of PSS and superfused to the experimental chamber for 20 min. The incubation of the cells with the dyes was followed by a 1-hour wash in PSS containing 1.7 mM CaCl 2 to allow time for de-esterification. The dye loading was performed at RT. The cells were then kept for 30 min at 37 C. Before imaging was commenced the cells were superfused with PSS containing 70 mM CaCl 2 and supplemented with 10 mM LaCl 3 to eliminate capacitative Ca 2C entry, unless stated otherwise. The cells were stimulated with 10 mM icilin. The intensity of fluo-4 or rhod-2 fluorescence (F) was normalized to the average fluorescence intensity in the images acquired before agonist application (F 0 ). The temporal profiles of the agonist-induced [Ca 2C ] c and [Ca 2C ] m transients are illustrated by the plots showing time course of the normalized fluo-4 or rhod-2 fluorescence intensity (F/F 0 ) averaged within confocal optical slice of the cell.

Superoxide assessment
HaCaT cells were transfected with empty vector (CTL), eTRPM8 plasmid or eTRPM8 C SOD1 plasmids. Cells were incubated in the medium supplemented with 2.5 mM CellRox® Deep Red reagent for 30 min at 37 C. After two washes, cells were incubated for 30 min at 37 C prior to confocal imaging. For each temperature and siRNA condition, the measurements were performed in 3 Petri dishes, sequentially mounted on the microscope stage at the ambient temperature corresponding to the pre-incubation temperature. In each Petri dish, confocal imaging of CellRox® Deep Red fluorescence was performed from 4 regions of interest, 230 £ 230 mm (1024 £ 1023 pixels) each. The same illumination intensity, photomultiplier gain and offset were used in all the experiments. Data were processed with ImageJ64 freeware. After setting offset (pixel fluorescence intensity below 20 a.u.) and the mean cell background (pixel fluorescence intensity 100 a.u.) in the control condition (cells grown at 37 C), specific to O 2 increase in the CellRox® Deep and was captured at wavelengths 505-530 nm. Rhod-2 fluorescence was exited by the 543 nm line of 15 mW helium/neon ion laser and detected at wavelengths 560-615 nm. The illumination intensity was attenuated to 0.5-6 % (depending on the laser line) with an acousto-optical tunable filter (Zeiss, Oberkochen, Germany). To optimize signal quality the pinhole was set to provide a confocal optical section 0.5-1.8 mm, depending on experimental protocol. To avoid any bleed-through of the fluorescence signal in multi-staining experiments, fluorochromes with well separated excitation and emission spectra were used and imaging was performed using the frame-by-frame multitrack mode of the confocal scanner: sequential acquisition via well-separated optical channels of the x-y images produced by fluorescence of different fluorochromes. The photomultiplier gain and offset in each optical channel were set individually to achieve similar signal intensity at each channel and remove sub-signal noise from the images. The adequacy of the imaging protocol applied to the multi-labeled myocytes was confirmed by control experiments on the mono-labeled cells.

Experimental solutions
In confocal microscopy experiments the cells were bathed in physiological salt solution (PSS) containing (in mM): NaCl 140, KCl 5, MgCl 2 1, glucose 10, HEPES 10; pH adjusted to 7.4 with NaOH. PSS was supplemented with 70 mM or 1.7 mM CaCl 2 , depending on experimental protocol, as described in the text.

Data analysis and statistical procedures
Each experiment was repeated at least 3 times independently. Data are expressed as mean § S.D when not indicated in legends. The data were analyzed and graphs plotted using Origin 5.0 software (Microcal, Northampton, MA, USA). InStat3 (GraphPad Software Inc., SanDiego, USA) was used for statistical analysis and the mean values were compared using either unpaired t-test with Welch's corrected test (2 groups) or One-way ANOVA with Dunnett multiple comparison post-test (3 groups). Statistical significances were: x p < 0.05.

Disclosure of potential conflicts of interest
No potential conflicts of interest were disclosed.