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Dispersion control considerations for multiphoton and non-linear laser microscopy applications

As multiphoton microscopy increases in popularity users with diverse backgrounds are exploring new applications for the technique. With the most recent 'turnkey' systems now on the market a typical user no longer has to be a laser physicist or engineer to employ a multiphoton system in their research. However, some basic understanding of the mechanisms of non-linear excitation will allow a user to optimize his multiphoton system for improved performance or extend it for new applications.