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Retroviral‐mediated gene transfer into human myeloma cells

Summary. We have studied retroviral‐mediated gene transfer into human myeloma cells, Bone marrow cells were obtained from four patients with advanced myeloma, where was heavily infiltrated with myelomatous plasma cells. Myloma cells were isolated by immunomagnetic separation, using the high‐affinity B‐B4 monoclonal antibody. Following separation, cells were transduced with the LN retroviral vector, which carries the gene for neomycin phosphotransferase, by incubation in cell‐free supernatant with or without a growth‐factor combination of IL‐3, IL‐6 and SCF. After infection, the cells were cultured for 9 d in RPIMI‐1640 and 10% FCS. either in the presence or absence of the neomycin analogue G418.