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Coastal sharks supply the global shark fin trade

Progress in global shark conservation has been limited by constraints to understanding the species composition and geographic origins of the shark fin trade. Previous assessments that relied on earlier genetic techniques and official trade records focused on abundant pelagic species traded between Europe and Asia. Here, we combine recent advances in DNA barcoding and species distribution modelling to identify the species and source the geographic origin of fins sold at market. Derived models of species environmental niches indicated that shark fishing effort is concentrated within Exclusive Economic Zones, mostly in coastal Australia, Indonesia, the United States, Brazil, Mexico and Japan. By coupling two distinct tools, barcoding and niche modelling, our results provide new insights for monitoring and enforcement. They suggest stronger local controls of coastal fishing may help regulate the unsustainable global trade in shark fins.


Introduction
Scientists have long sought diagnostic tools to improve monitoring biodiversity in wild ecosystems and in markets [1,2] at a scale that matches their occurrence and exploitation [3,4]. Such tools could be vital for assessing the wildlife trade, where the species and geographic origin are often difficult to diagnose from traded products [5]. For shark fins alone, the trade is valued at nearly US$ 400 million and kills perhaps 100 million sharks annually [6,7]. Efforts to monitor the shark fin trade, however, have been impeded in part by extensive processing of the marketed products (figure 1). Though some fins are traded intact, many are traded with the skin and other morphological features removed, and mixed in stockpiles, defying easy cataloguing.
Advances in genetic sequencing, marker selection and global sequence libraries have increased the output and lowered the cost of diagnostic species identification of wildlife products. Separately, improvements in species distribution modelling [8][9][10] now allow probabilistic mapping of species occurrence from derived environmental niches. Such niche models have been successfully applied to marine fisheries, for example, to reduce bycatch and predict longline fishing effort (e.g. [11,12]). For the shark fin trade, coupling the two approaches into a single analysis may help narrow a broad problem by describing the most probable shark fishing locations, potentially revealing geographic, which will improve monitoring and conservation.
PCR-based sequencing of individual fins provided the first species key to interpret trade records and estimate species composition. One pioneering analysis [3] of fins from the Hong Kong auction, which accounts for half of the global fin trade, revealed the dominance of pelagic sharks (Prionace glauca, Carcharhinus longimanus, Isurus spp. and Alopias spp.) and focused management on high-seas fishing. However, the method was difficult to scale up and provided little information on rare species. A DNA barcoding approach using BOLD and BLAST taxonomy databases [13] with extensive sequence libraries provides an improved ability to identify larger numbers of species. Using this approach, four recent studies [13][14][15][16] revealed the source species of over 5000 individual shark fins from markets in Hong Kong, Vancouver, San Francisco and northern Brazil. These studies provide a novel influx of robust information, identifying a wide variety of threatened species, and marking a new and increased ability to identify processed market samples to the species level.
Here, we combine these existing barcoding data with species distribution models (SDMs) to understand the geographic sources of traded shark fins. This generates a probability surface of the global ocean that describes the likely locations fished to produce the market fins. Finally, we assess the relative importance of areas inside and outside Exclusive Economic Zones (EEZs) and accumulate and rank each nation's contribution. Our results revise the estimates from 20 years ago, suggesting that the greatest opportunities for regulating the shark fin trade are in coastal regions.

Methods
Published studies [13][14][15][16] used cytochrome oxidase (COI) sequencing to provide robust species identifications for 5327 shark fins sampled at markets in Brazil, Canada, China and the United States (electronic supplementary material, table S1 and figure S1). Previous analyses generated SDMs for 51 of these species from open-access occurrence data at Global Biodiversity Information Facility, FishBase and Ocean Biodiversity Information System. Each SDM is the ensemble of four environmental niche models (BioClim, Boosted Regression Trees, Maxent and Artificial Neural Networks [8]) and displays the probability of occurrence globally at a 0.5°× 0.5°resolution. To account for potential sampling biases in the source data, we conducted randomized cross-validations for all SDMs and evaluated model performance (see electronic supplementary material, table S2). AquaMaps [17] provided SDM outputs for eight additional species (see electronic supplementary material, table S1) using similar methods.
We simulated probability maps of shark fishing by integrating the barcoding results and SDMs. For each market study, w i is the compositional proportion (w) for each identified species (i). With each SDM raster, we conducted w i × 10 6 Bernoulli trials in R [18] where the dplyr package [19] 'sample_n' function randomly selected pixels and the R 'binom' function queried them (1 = presence, 0 = absence). This routine adds stochasticity and simulates fishery captures by reducing continuous probability in the SDM surface to discrete events. We summed all SDM trials for each barcoding study and rescaled the accumulated surfaces from 0 to 1 for standardized comparison. Next we mapped results, summed and compared probability densities within and outside EEZs, and calculated and ranked the total contributions from each sovereign nation. All analyses and visualizations were conducted in the R environment. All scripts and data are provided open access (https://osf.io/xvrmk/, [20]).

Results and discussion
Our analyses suggest most harvested shark fins originate within EEZs rather than in high-seas regions, counter to previous assessments [3]. Abundant and widespread species such as blue sharks (P. glauca) remain the dominant species in fins from the Hong Kong market hub [15]. However, even there, genetic barcoding revealed an additional 40 range-restricted coastal species (electronic supplementary material, figures S1 and S2), while studies from other markets [13,14,16] show a greater proportion of coastal sharks. Figure 2a plots the probability of occurrence for all species, according to fin identifications in four market studies. The similar geographic pattern of the Hong Kong, Vancouver and San Francisco market sources [13,15,16] reflects that China is the established aggregating node that receives, processes and supplies a majority of fins to global markets [16]. The Brazil-based study [14] sampled fins in a series of local wet markets and indicates a dominance of fishing activity in coastal Brazil and the Caribbean (figure 2a,c). Unsurprisingly, the relative frequency of modelled shark fishing within EEZs to the high seas (figure 2b) is greatest in the Brazil market (48 : 1). Yet even in the recent Hong Kong study, shark fins originated within EEZs by a 2 : 1 ratio.
Since the largest portion of shark harvest originates within EEZs, there is an urgent need for nations to adopt enforceable conservation measures within their jurisdictions. The findings also suggest that progress is not limited to international negotiations over high-seas fishing and can also be targeted to EEZ regions with more jurisdictional oversight. Figure 2b shows that probability of origin declines steeply with frequency (note the log-scaled y-axis), as the niche models have focused the likely locations of shark fishing to royalsocietypublishing.org/journal/rsbl Biol. Lett. 16: 20200609 coastal zones (figure 2a). Small-scale vessels operating in coastal waters, however, present multiple complications for monitoring, including enforcement gaps, stockpiling and transhipment [5]. Progress on these persistent challenges within the EEZs of Australia, Indonesia, the United States, Brazil, Mexico and Japan (figure 2c; electronic supplementary material, table S3) may be especially effective. These results differ starkly from previous summaries of the shark fin trade that relied on official customs statistics [7]. Such records are widely considered unreliable, double count re-export data, and do not represent the geographic origin where shark harvests occurred [7]. Our approach helps to resolve these issues.
Despite some geographic differences in the sourcing regions of the four markets, the barcoding results consistently revealed a high number of threatened and rare species. Averaged across studies, 52% of species identified (range 47-60%), and 61% of fins (range 35-75%) came from species classed as either threatened (CR, EN or VU) or 'data deficient' (DD) by the IUCN (see electronic supplementary material, table S1). Some species are further protected by the Convention on the International Trade in Endangered Species and various national policies. Previously, genetic analyses of the global composition of shark species were limited to the detection of the most common species that were also morphologically identifiable [3]. That half of traded fins from recent barcoding studies are from species of conservation concern reveals a pressing need for increased monitoring, management and enforcement of the shark fin trade.
Some shark fisheries are considered sustainable. The spiny dogfish (Squalus acanthias) for example has been suggested as a model shark fishery [21] and potentially could supply shark fin markets. However, S. acanthias is harvested for meat and does not produce marketable fins, and there existed zero S. acanthias identifications in the barcoding analyses we compiled. Other reportedly sustainable shark fisheries [21] represent just a small minority of fin identifications from markets (electronic supplementary material, table S1). Sustainable choices to supply markets and consumer demand for shark fins may therefore remain elusive, furthering support for strong controls on the shark fin trade [22,23].
We attribute the identification of more species and rare species to growth in barcoding libraries and attribute the coastal concentration of shark fishing (figure 2) to this influx of species and the niche-based SDMs. By proportionally weighting suitable habitats, the SDMs provide a more informed assessment of where encountering those species and their harvests most likely occurs [11]. However, as the royalsocietypublishing.org/journal/rsbl Biol. Lett. 16: 20200609 chronic exploitation of pelagic sharks in high-seas fisheries has collapsed many shark populations [24], our results here may also reflect a serial shift from distant fleets to near-shore fishing. In addition, expanded sampling beyond Hong Kong retailers may have revealed previously undetected regional differences in supply chains. Either way, this raises new concerns as near-shore shark populations have also seen dramatic declines [25], are typically less abundant, have smaller geographic distributions and often have less management [15]. Data accessibility. All data and code for this study are available at a thirdparty open-access repository (https://osf.io/xvrmk/) [20].