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Immunogold visualization of actin near the coated pits at the apical surface of human mammary epithelial cells.

This ultrastructural study of both the normal human breast tissue and differentiated mammary carcinoma (NOS) epithelial cells has revealed pictures demonstrating luminal receptor-mediated endocytosis. By application of immunogold anti-actin labeling, actin surrounding the fusion ring of coated pits was visualized. However, the coated membrane was not actin labeled. We suggest that association of the actin with coated pits may evidence for its participation in pinching off of the coated vesicles.