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Isolation of Stigmast- 4 -ENE-3-One and Gamma- Sitosterol from the Aerieal Part of Synedrella Nodiflora Linn (Asteracae)

Pulverized aerial plant material (100 g) of Synedella nodiflora Linn was exhaustively extracted with petroleum spirit, chloroform, ethyl acetate and methanol, the extracts was concentrated in- vacuo using rotary evaporator at 40 o C. The ethyl acetate extract was subjected to column chromatography analysis to yield various fractions which were combined based on their thin layer chromatography analysis to give SEA. This was further purified by the preparatory thin layer chromatography (PTLC) to give SEA-1. The structure of the isolated compounds was established by IR and GC-MS spectroscopic analysis and by direct comparison of the data obtained with those reported in literature to be stigmast- 4 -ene-3-one and gamma-sitosterol. The compounds are reported for the first time from this plant. General phytochemical screening of the aerial parts of Synedrella nodiflora revealed the presence of flavonoids, alkaloids, tannins, steroids, glycoside and cardiac glycoside.