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Quantitative microscopical and confocal laser scanning microscopy for intermediate endpoint biomarkers in breast cancer: Potential and reproducibility

Diagnostic quantitative pathological (QP) determinations are increasingly used in our hospital. The number of requests for QP for reference materials is rising rapidly. This is understandable; quantitative assessments have a strong prognostic value and can be very reproducible, depending on the care taken with a number of factors including cell and tissue processing, application of the appropriate stains, and the measurement protocol used. As to the latter, systematic random sampling gives the best intra‐ and interobserver agreement (with correlation coefficients between observers for certain features ≥0.94).