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Evaluation of the Listertest™ Method for Quantitation of Listeria monocytogenes in Seafoods.

The U.S. Food and Drug Administration most probable number (MPN) method and the Listertest™ (immunomagnetic capture) were used to enumerate Listeria monocytogenes on laboratory-inoculated cooked crabmeat ( Callinectus sapidus ) and cold-smoked salmon ( Salmo salar ). The products were inoculated with <1.0 to 4.0 log CFU of L. monocytogenes per g and analyzed immediately. In the analyses of crabmeat, the results obtained by the MPN method were significantly lower (P < 0.05) than counts produced by the Listertest™. There was no significant difference (P < 0.05) between results produced by MPN and the Listertest™ in analyses of smoked salmon. The methods had the same variance in the quantitation of L. monocytogenes in these seafood products.