{'fraction_non_alphanumeric': 0.042000372508847084, 'fraction_numerical': 0.02365431178990501, 'mean_word_length': 5.203928365106875, 'pattern_counts': {'":': 0, '<': 2, '<?xml version=': 0, '>': 1, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
COVID-19 and Endocrine System: A Cross-Sectional Study on 60 Patients with Endocrine Abnormality

Objective COVID-19 is an infectious disease that has become pandemic with a high mortality rate. This study aims to provide new insight into the relations between SARS-CoV-2 and the Endocrine system. Materials and Methods In this cross-sectional study, we have hospitalized 60 patients with a positive SARA-CoV-2 PCR test. The information of complete blood count and endocrine hormones was obtained when the patients were admitted to the hospital or for a maximum of 4 days onset the hospitalization. Results Of 60 patients with COVID-19, forty-four (73.33%) had at least one abnormality mean item >×3. In total, 26 (43.33%), 21 (35%), 18 (30%), 13 (21.67%), 31 (51.67%), 12 (20%), 30 (50%), 25 (41.67%) patients having estradiol, follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, progesterone, testosterone, cortisol and thyroid stimulating hormone (TSH) abnormal test results, respectively. There was no change in creatinine levels. FSH has shown drastic changes in both sexes’ intensity (F: 769, P<0.0001). Although TSH had many abnormalities in women, analysis has shown no significant P value (P=0.4558). Furthermore, prolactin and testosterone mean level in men and the estradiol mean level in women have shown no significant P value (P=0.2077, P=0.1446, P=0.1351, respectively). Conclusion Results suggest that COVID-19 affects directly or non-directly glands and related hormones.


Introduction
Coronaviruses are a common virus within animals and humans that cause multiple system infections in both species, predominantly humans respiratory tract infections, such as severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS). Coronaviruses also cause enteric, hepatic, and neurologic diseases (1)(2)(3). Since late December 2019, a newfound virus has become prevalent in Wuhan, China, previously described as (2019-nCoV), which subsequently affected all countries worldwide (4,5).
The worldwide health organization labeled the COVID-19 as a pandemic worldwide that has led to thousands of deaths, albeit most of the cases have mild symptoms, more severe symptoms have caused respiratory failure, septic shock, and multiple organ failure dysfunctions (6). It appears that 93.1% of the sequence identity of the spike gene of the virus is relative to the RaTG13 of Bat coronaviruses. However, the other SARS-CoV and SSRS-CoVs have less than 80% manifested sequence identity (7). A homometric spike glycoprotein with S1 and S2 subunits in each spike monomer operates as cellular receptors to bind the hostage. A cascade of events occurs by binding the receptors leading to the fusion between viral membrane and cell. Cryo-EM studies of the SARS-CoV spike with ACE2 cell receptor have shown that glycan-RBD (a hexapeptide in the receptorbinding domain) enforces the separation of the S1 with ACE2 as a necessary action for membrane fusion through actuating the S2 subunit from mutable perfusion to a more post-fusion state (8)(9)(10)(11)(12)(13). A required regularization of the renin-angiotensin system, named angiotensin-converting enzyme 2 (ACE2), has been known as a homolog of the metalloprotease angiotensin-converting enzyme ACE (14,15).
The expression and presence of the ACE2 within the internal organs may assume to be the potential path of the entrance of the COVID-19 virus. Highly enriched expression and distribution of ACE2 at the surface of type 2 alveolar cells of the lung, oral mucosa, tongue, stratified and upper esophagus epithelial cells, colon and ileum absorptive enterocytes, myocardial cells, cholangiocytes, proximal tubule cells of the kidney, bladder urothelial cells, seminiferous duct cells in the testis and leydig cells have been detected (16)(17)(18)(19)(20)(21). These uncoverings demonstrate that organs with high expression of ACE2 receptors are exposed to the high risk of 2019-nCoV infection (18). Moreover, hormonal disorders can amplify cytokine storms and organ failure (22).
However, the data of other organs has not been comprehensively analyzed. Hence, this study aims to report the hormonal sex, adrenal, ovary, hypothalamus, thyroid, and pituitary gland function and their parameters in patients with COVID-19 hospitalized to the Bagher-Al-Olum hospital in Ahar, Iran. More potential therapies would be recognized with a better concentrate on pathogenesis and affected human physiology, which might intercept glands failure in patients with SARS-CoV-2.

Study criteria and design
We conducted a cross-sectional study of patients admitted to the Bagher-Al-Olum Hospital of Ahar. As the WHO interim guidance (23). From November 12, 2020, to December 18, 2020, 85 infected patients were identified; however, only 60 were placed in this study. All of the patients were in reproduction age. Those who had abnormal CT-Scan, and CBC and Diff test results with a signed consent form, from admission to November 12, 2020, were enrolled in the study. Also, we extracted 60 normal laboratory test results from the hospital database (HIS) to set as the control group for reaching an accurate comparative study. Women with menstrual disorders and patients using medications that may affect hormonal results were excluded from the study. This study has taken approval from Tabriz University of Medical Sciences Ethics Committee, Iran (IR.TBZMED.REC.1399.129).

Confirmation and severity of SARS-CoV-2
Hospitalization of patients was based on confirmation of positive real-time polymerase chain reaction (PCR), chest CT-Scan, and abnormal CBC and Diff tests. All patients stated no history of glands disease, and their odd results were likely due to SARS-CoV-2 disease. Nasopharyngeal and throat specimens were collected by swab from dubious patients to evaluate their E gene expression by RT-PCR. According to the Infectious Diseases Society of America/ American Thoracic Society (IDSA/ATS) guideline (24), Pneumonia was defined. Combinations of azithromycin, Hydroxychloroquine, Kaletra, Vitamin C, and Interferon 1-B were applied for treatment.

Statistical analysis
GraphPad Prism version 8.4.3 (San Diego, California, USA) was used for statistical analysis. Median for continuous variables were compared using independent group t tests when the data were normally distributed; confidence interval (CI) and Significance were set as a P<0.05 for each group; otherwise, the Mann-Whitney U test was used. Also, an ANOVA test was performed to compare the mean among several independent groups, and F statistics were reported for the ratio of changes Between Groups to Within Groups.

Clinical characteristic of patients with COVID-19
Blood samples due to precise analysis of the cortisol were collected at 8 AM.

Discussion
Our study has comprehensively investigated most of the hormones, that had not been measured during Covid-19 disease. Based on our findings, most of the studied female cases had abnormal changes in their hormones compared with the studied males. The first hormone that showed increasing, and decreasing changes in both sexes was estradiol. Many reasons indicate the absence of estrogen is a consequence of dysfunction or toxicity of the hypothalamus-pituitary axis and ovary (25,26), leading to the coronary artery and renal diseases, osteoporosis, changes in verbal memory performance and mood, and IL-6 production, which have been recognized as a critical factor in cytokine storm that occurs in Covid-19, osteoclasts, and dysgeusia (27)(28)(29)(30)(31)(32). Likewise, increasing estradiol levels will affect the thyroid and enhance iodide concentration, thrombotic, ischemic stroke, and elevations of breast cancer risk (33)(34)(35)(36). Firstly, Estradiol's abnormal levels in patients might directly result from the damaged ovary, hypothalamus-pituitary axis, FSH, and LH abnormalities. Secondly, maybe as a derivative result of dysgeusia led by the virus itself or antiviral drugs or a combination of both. The next and more forgotten hormone in men is progesterone, which showed only decreasing changes. Progesterone is an essential hormone in men that influences spermatogenesis, sperm capacitation/acrosome reaction, and testosterone biosynthesis in the Leydig cells. Besides, the nervous system has the capacity to bio-convert progesterone into its active metabolite allopregnanolone. Other progesterone effects include blocking gonadotropin secretion, sleep improvement, and effects on tumors in the central nervous system (CNS) (meningioma, fibroma), and effects on the immune system, cardiovascular system, and kidney function, adipose tissue, behavior, and respiratory system (37,38). As we know, in women, progesterone saves the pregnancy, regulates the period, and the ovary produces the most amount of it; also, adrenals generate a slight amount of progesterone. The abnormal amount of progesterone in both sexes is probably a result of impairment of the ovary, adrenal, or hypothalamuspituitary axis.
Both genders (male and female) had almost equal abnormalities, suggesting that adrenal and or hypothalamus-pituitary axis are most likely to be involved in the virus attack, rather than women hormons secretion. Testosterone was among those hormones with fewer fold changes. Still, there is a need to enhance the statistical community to determine whether it is an effect of SARS-CoV-2 or not since it is an essential hormone in reproductive health. Moreover, the levels of FSH, LH, TSH, and prolactin showed significant changes. The results of the current study suggests that hypothalamuspituitary axis was infected and affected by the virus, which consequently leads to cardiovascular disease, blood pressure, and Metabolic disease (39). However, a sudden increase in estrogen and progesterone can cause negative feedback of FSH and LH hormones. Also, the abnormal TSH level might be negative feedback toward T3 and T4 amount and probably reflects the thyroid gland injury. The excess of Cortisol secretion on the immune system, blood pressure, fat deposition, and symptoms and disease is well documented. Therefore, the abnormal level of cortisol and progesterone have been found in this study confirmed the hypothesis of adrenal damage. We will next determine whether these changes are the results of cytokine storm, Stress caused by fear of illness, or the amount of CRH and ACTH released by the hypothalamus and pituitary glands. There is a need to measure the CRH, ACTH, GNRH, T3, and T4 in more samples to endorse the damage of cerebral glands, thyroid, and virus access into the brain.

Conclusion
These results suggested that COVID-19 affects directly or non-directly glands and related hormones.