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Identification of Escherichia coli 01 57:H7- strains from pigs with postweaning diarrhoea and amplification of their virulence marker genes by PCR

Escherichia coli isolated from pigs with postweaning diarrhoea were examined by PCR for the presence of the 0157 rfb gene responsible for the biosynthesis of E coli 0157 lipopolysaccharide. Among the 372 isolates tested, 38 (10.2 per cent) were of the 0157 serogroup, but none of these possessed the H7 determinant. Further analysis of the E coli 0157 isolates revealed that seven of them had the genes responsible for the production of Shiga toxin 1 and eaeA intimin, four other strains had genes responsible for the production of Shiga toxin 2, and four other strains were positive for the enterohaemolysin gene.