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Multifunctional Supramolecular Assemblies with Aggregation-Induced Emission (AIE) for Cell Line Identification, Cell Contamination Evaluation and Cancer Cell Discrimination.

It's undoubted that the important role of cells in biology and medicine, but the worldwide misidentified and cross-contaminated cell lines have caused many troubles in the related fields. Herein, three kinds of supramolecular AIE (aggregation-induced emission) nano assemblies were constructed by host-guest interaction between tetraphenylethene (TPE) derivatives and cucurbit[8]uril (CB[8]). Based on the recognized mechanism of AIE, the TPE derivatives could achieve stronger fluorescence emission and higher fluorescence quantum yield, after assembling with CB[8]. Moreover, the constructed supramolecular AIE complexes obtained well-confirmed nanostructures and exhibited different size and shape. Correspondingly, they generated characteristic biological properties and fluorescence enhancement upon cells. Inspired by the concept of Big Data Analysis (BDA), these fluorescence signals were further transformed into a unique fingerprint of cells via linear discriminant analysis (LDA). Immediately, we realized the veracious identification between a normal cell line, two cancer cell lines and two metastasize cancer cell lines at qualitative analysis. More importantly, it was well used to monitor the evaluation of cross-contaminated cells and the discrimination of cancer cells. As a proper bio-application of ideal supramolecular nanomaterials, this system was easy to learn and applied, and the whole procedure was kept in 20 mins, without cell disruption, centrifugation, or washing steps.