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EVALUATION OF EFFECTIVENESS OF SYNCHRONIZATION METHODS OF CELL DIVISION IN YEAST SACCHAROMYCES CEREVISIAE.

Synchronization of cell division in cultures of yeast Saccharomyces cerevisiae is widely used in research on the regulation of gene expression and biochemical processes in eukaryotes at different stages of the cell cycle. Here, we compare the efficiency of modern most commonly used methods to achieve and assess the degree of synchronization of cell division in yeast. Block-and-release methods with alpha-factor, hydroxyurea, nocodazole, cdc28-4 mutation are described in detail with practical notes.