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Comparison of the reactivity of various epithelial substrates for the titration of pemphigus antibodies by indirect immunofluorescence.

Indirect immunofluorescence was used to compare the reactivity of thirteen different squamous epithelia for the titration of serum antibody in five patients with active pemphigus vulgaris. The most useful epithelia, as judged by pemphigus antibody titres, were, in order of effectiveness, human skin, human tonsil, monkey oesophagus, monkey lip, guinea‐pig oesophagus, rabbit‐oesophagus, guinea‐pig lip and rabbit lip.