{'fraction_non_alphanumeric': 0.03602305475504323, 'fraction_numerical': 0.010086455331412104, 'mean_word_length': 5.205357142857143, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Internalization of Shigella flexneri into HeLa cells occurs without an increase in cytosolic Ca2+ concentration

Pathogenic Shigella flexneri invades epithelial cells through directed phagocytosis. The role of intracellular free calcium ([Ca2+]i) as a signal for this process was tested. No variation in the [Ca2+]i could be detected by using the fluorescent indicator fura 2 to measure the [Ca2+]i in HeLa cells during the invasion process. In addition, neither clamping nor maximal decreasing or increasing of the [Ca2+]i of HeLa cells had any effect on their susceptibility to invasion. These data demonstrate that [Ca2+]i is not a signal for S. flexneri directed phagocytosis in HeLa cells.