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A Widespread Bacterial Secretion System with Diverse Substrates

ABSTRACT In host-associated bacteria, surface and secreted proteins mediate acquisition of nutrients, interactions with host cells, and specificity of tissue localization. In Gram-negative bacteria, the mechanism by which many proteins cross and/or become tethered to the outer membrane remains unclear. The domain of unknown function 560 (DUF560) occurs in outer membrane proteins throughout Proteobacteria and has been implicated in host-bacterium interactions and lipoprotein surface exposure. We used sequence similarity networking to reveal three subfamilies of DUF560 homologs. One subfamily includes those DUF560 proteins experimentally characterized thus far: NilB, a host range determinant of the nematode-mutualist Xenorhabdus nematophila, and the surface lipoprotein assembly modulators Slam1 and Slam2, which facilitate lipoprotein surface exposure in Neisseria meningitidis (Y. Hooda, C. C. Lai, A. Judd, C. M. Buckwalter, et al., Nat Microbiol 1:16009, 2016, https://doi.org/10.1038/nmicrobiol.2016.9; Y. Hooda, C. C. L. Lai, T. F. Moraes, Front Cell Infect Microbiol 7:207, 2017, https://doi.org/10.3389/fcimb.2017.00207). We show that DUF560 proteins from a second subfamily facilitate secretion of soluble, nonlipidated proteins across the outer membrane. Using in silico analysis, we demonstrate that DUF560 gene complement correlates with bacterial environment at a macro level and host association at a species level. The DUF560 protein superfamily represents a newly characterized Gram-negative secretion system capable of lipoprotein surface exposure and soluble protein secretion with conserved roles in facilitating symbiosis. In light of these data, we propose that it be titled the type 11 secretion system (TXISS).

functions, including acquisition of nutrients (1,2), interaction with host cells (3), and specificity in host range and tissue localization (4). Possibly due to the complexity of bacterial membranes and the breadth of biophysical characteristics of secreted proteins, there is no singular export pathway.
Bacteria have two broadly distributed export systems, including the inner membrane spanning Sec and twin arginine translocation (Tat) systems which are shared between Gram-positive and Gram-negative bacteria. Diderms require additional secretion systems for transport into and across the outer membrane. The type I, II, V, IX, and X secretion systems work to transport substrates across that outer membrane, while types III, IV, and VI go one step further, moving effector proteins across the outer membrane and directly into another organism (5)(6)(7). Despite the many secretion systems described thus far, there are still a number of secreted proteins which are known to contribute to symbiosis but for which no transport system is known. Here we describe a machinery present throughout the phylum Proteobacteria that is responsible for secreting soluble proteins and lipoproteins from the periplasm across the outer membrane.
Recently, the Slam (surface lipoprotein assembly modulator) mechanism of lipoprotein surface tethering was identified in Neisseria meningitidis (8,9). Slam proteins containing the b-barrel DUF560 (domain of unknown function 560) (also termed SlipAM domain) (10) are required for surface presentation of certain lipoproteins, including those that capture metal-carrying compounds used by hosts to sequester nutrients (8). Two N. meningitidis Slam proteins have been characterized, with distinct lipoprotein substrates which contribute to symbiosis. Slam activity also has been demonstrated for DUF560 representatives from pathogens Pasteurella multocida, Moraxella catarrhalis, and Haemophilus influenzae (8,9). However, most Slam homologs have no bioinformatically predicted substrate thus far, and one study has found that N. meningitidis Slam1 can surface expose unprocessed factor H binding protein (fHbp) single nucleotide polymorphism (SNP) variants (11). Thus, the full functional potential of DUF560 proteins is not yet known.
The DUF560 homolog NilB is a host association and species specificity factor in the nematode symbiont Xenorhabdus nematophila, a proteobacterium in the family Morganellaceae (12)(13)(14). A screen for X. nematophila mutants defective in colonizing Steinernema carpocapsae intestines revealed the nematode intestinal localization (nil) locus (14,15). The nil locus contains the genes nilB and nilC, each of which is independently necessary for colonization of nematodes. Biochemical and bioinformatic analyses have established that NilC is an outer membrane-associated lipoprotein, and NilB is an outer membrane b-barrel in the DUF560 family with an ;140-amino-acid periplasmic N-terminal domain that contains tetratricopeptide repeats (15)(16)(17)(18).
To begin to understand the functional range of DUF560 proteins, we assessed ecological distribution, genomic context, and relatedness. We experimentally examined the X. nematophila DUF560 homolog HrpB, which is predicted to transport an unlipidated protein. Finally, to better understand the potential role of DUF560 proteins in host-symbiont interactions, we analyzed distribution among symbiotic Xenorhabdus. Our data demonstrate that biological activities of the DUF560 family extend beyond lipoprotein surface presentation and constitute the type 11 bacterial secretion system (TXISS) which, like the type II secretion system, is capable of acting on membraneanchored or soluble proteins ( Fig. 1) (19).
(This article was submitted to an online preprint archive [20].)

RESULTS
TXISS cluster according to environment. Using homology to NilB or Slam proteins, previous work identified a wide distribution of DUF560 proteins within mucosa-associated bacteria (9,14,15). To quantifiably delineate subfamilies within the TXISS, we generated a sequence similarity network (SSN) using the Enzyme Function Initiative toolset (EFI) (21)(22)(23) and annotated it to highlight environmental source or taxonomic grouping of microbes containing DUF560 homologs ( Fig. 2; see also Table S1 in the supplemental material). In this analysis, sequences with high identity ($40%) were gathered into nodes and connected with edges based on sequence similarity. Using all homologs in Interpro 73 and UniProt 2019-02, we identified 10 major clusters of TXISS proteins. Cluster 1 was chosen for in-depth analysis as it contained the majority of nodes in the network (62.4%) and could be clearly divided into three subclusters (1A, 1B, and 1C) using force-directed node placement (Fig. 3). The remaining nine clusters displayed a preponderance of water-and soil-associated organisms and contained no characterized proteins (see Table S1 for details). Consistent with our previous observations (15), the majority of cluster 1 nodes (75%) comprise sequences from animal-associated isolates, while another 20% contain sequences from marine, freshwater, soil, or built-environment isolates.
The division of cluster 1 nodes strongly reflected environmental origin (Fig. 3A). Subcluster 1A almost exclusively comprises animal-associated bacteria and contains all previously characterized TXISS, which separate according to the predicted substrate when analyzed with higher stringency (see Fig. S1 in the supplemental material). Subclusters 1B and 1C have no previously characterized representatives. Subcluster 1B contains a mixture of sequences from host-associated and free-living bacteria, while subcluster 1C contains sequences largely from environmentally isolated Sphingomonadaceae. Subclusters 1A and 1B correlate poorly with taxonomy (Fig. 3B). For example, cluster 1 contains 81 nodes with Moraxellaceae sequences. Of these, 79% are animal associated and are enriched in subcluster 1A, while 12.3% are environmental isolates and are enriched in subcluster 1B. These data demonstrate a correlation with lifestyle (e.g., free-living versus host associated) as opposed to taxonomy and suggest that subclusters indicate divergent functions.
TXISS cluster according to substrate. The Slam acronym was defined on the basis that DUF560 homologs from N. meningitidis facilitate surface exposure of lipoproteins, such as transferrin binding protein B (TbpB), lactoferrin binding protein B (LbpB), hemoglobin/haptoglobin binding protein A (HpuA), and fHbp, which are frequently encoded nearby (8,9). Yet the lipid tail is not essential for Slam-dependent surface exposure of a target (8,11,24), prompting us to consider whether cooccurrence with lipoproteins is a constant. We used the EFI Genome-Neighborhood Tool (21,23,25) to assay the genomic context of cluster 1 members. This analysis corroborated previous work demonstrating genomic association of DUF560 proteins with TonB, TonB-dependent receptors, and proteins that have a Pfam TbpB_B_D domain, which will be referred to hereafter as TbpBBD (9,26).
Given the prevalence of TbpBBD domain-containing genes in the neighborhoods of DUF560 genes, and their known occurrence in lipoproteins exposed by Slams, we examined their open reading frames (ORFs) for potential patterns. Using a combination of genome-neighborhood-network data (23), Rapid ORF Description & Evaluation Online (RODEO) data (27), and manual annotation, we analyzed 851 unique TbpBBD domain proteins coinherited with cluster 1 DUF560s (Table S2). The majority (75.1%) of TbpBBD-bearing proteins associated with subcluster 1A have a signal peptidase 2 (SPII) signal peptide, indicating lipidation, and two TbpBBD domains, similar to TbpB in N. meningitidis (9). These are referred to here as TbpBBD lip (lipidated TbpBBD). In contrast, the TbpBBD-bearing proteins associated with subcluster 1B are almost exclusively predicted to be soluble proteins (97.8%) with signal peptidase 1 (SPI) signal peptides and a single TbpBBD domain, similar to hemophilin in Haemophilus haemolyticus. Hereafter we refer to this class of proteins as TbpBBD sol (for soluble TbpBBD) ( Fig. 3C and Fig. 4). TbpBBD lip and TbpBBD sol proteins are predicted to be translocated across the inner membrane through the Sec secretion pathway. However, at least some TbpBBD sol proteins have similarities to Escherichia coli OmpA and DsbA, which can be secreted through either Edges were drawn using an alignment score of 38, and any sequences which shared $40% identity were placed in a single node to allow the separation of clusters. Each node represents a group of highly similar sequences, with edge darkness demonstrating similarity, and the distance between nodes being determined via the Fruchterman-Reingold algorithm to optimize edge lengths (59). Each node was color coded to show the isolates' environmental origin(s) (A) and taxonomic class (B). N/A, not applicable.
Biochemical and structural evidence support the conclusion that the TbpBBD sol protein hemophilin is a soluble secreted protein that binds free heme and facilitates heme uptake (29). Three-dimensional homology modeling (Phyre 2 ) was used to visualize potential structural similarities between hemophilin and several TbpBBD sol proteins: a previously described homolog X. nematophila XNC1_0075 and two of its most closely related homologs, Providencia rettgeri PROVRETT_08181 and Proteus mirabilis WP_134940027.1 (15,(30)(31)(32) (Fig. S2). No high confidence models were found for the first ;50 residues, which correspond to the position of the variable N-terminal handle domains of TbpBBD lip proteins. However, the structures of the central regions and the C-terminal TbpBBD b-barrel domains were predicted with high confidence (.99%) based on hemophilin. In light of sequence and structural level similarities, we predict two functional TXISS subfamilies; TXISS-1A members flip TbpBBD lip substrates across the outer membrane, and TXISS-1B members secrete TbpBBD sol substrates into the extracellular milieu.
TXISS-1B activity reconstructed in vivo. To experimentally evaluate our prediction that TXISS-1B can secrete TbpBBD sol substrates, we investigated the heme receptor protein (Hrp) locus of X. nematophila. This locus, conserved across the Xenorhabdus genus, encodes TonB, a TonB-dependent heme receptor HrpA (XNC1_0073), the TXISS-1B homolog HrpB (XNC1_0074), and its predicted TbpBBD sol substrate HrpC (XNC1_0075) (15), the hemophilin homolog described above (29). Specifically, we sought to test whether HrpB mediates secretion of HrpC. Immunotagged HrpC was expressed with or without immunotagged HrpB in E. coli. Whole-cell and culture supernatant fractions were separated and analyzed by immunoblotting with anti-FLAG antibody (33). Whole-cell lysates demonstrated equivalent expression of HrpC in all treatments (Fig. 5A). In the presence of FLAG-tagged HrpB (FLAG-HrpB), the levels of HrpC-FLAG detected in culture supernatants increased 9.9-fold at 1 h postinduction and 17.0-fold at 2.5 h postinduction ( Fig. 5A and B), demonstrating that secretion of HrpC is mediated by HrpB.
A trivial explanation for these data could be that HrpB expression leads to cell lysis. The supernatant protein profile revealed by Coomassie blue staining of sodium dodecyl The Type 11 Secretion System ® sulfate-polyacrylamide gels (SDS-PAGs) did not indicate cell lysis or nonspecific protein secretion ( Fig. S3). To further rule out this possibility, we created an HrpC2glutathione S-transferase (GST) fusion protein and coexpressed it with FLAG-HrpB. Previous observations had demonstrated that GST-fused TbpB is not surface exposed by Slam1 (8). Consistent with this finding, the levels of HrpC-GST in a culture supernatant were unaffected by coexpression with FLAG-HrpB, supporting the conclusion that expression of the outer membrane protein HrpB does not cause bacterial lysis ( Fig. 5A and B) and moreover demonstrating that HrpB cannot secrete its substrate when HrpC is fused to a 26-kDa protein.
In the absence of Slam1, a fraction of the cargo protein fHbp is surface exposed when expressed in E. coli BL21(DE3) (34,35). Similarly, we found that some HrpC-FLAG and HrpC-GST reached the supernatant in the absence of FLAG-HrpB. Furthermore, FLAG-HrpB was unexpectedly detected in the supernatant fraction (Fig. 5B). To distinguish soluble supernatant proteins from those that may be associated with insoluble membrane components (e.g., outer membrane vesicles [OMVs]), we depleted insoluble components of the supernatant via ultracentrifugation and tested the clarified soluble fraction for Hrp proteins. Effectively all HrpB in the supernatant was removed with ultracentrifugation, suggesting it is localized in OMVs ( Fig. 5C) (36). Also, in the absence of HrpB coexpression, the levels of HrpC detected in the supernatant were reduced upon removal of insoluble material, while supernatant levels of HrpC detected after coexpression with HrpB were not affected by removal of insoluble material (Fig. 5D). We conclude that HrpB is membrane bound and that it secretes soluble HrpC protein into the extracellular milieu.
Host environment drives TXISS class. Having established that DUF560 homologs represent a bona fide secretion system, we next used the Xenorhabdus system to expand on our observation that the presence and type of TXISS correspond to environmental niche. Xenorhabdus bacteria are species-specific obligate mutualists of Steinernema nematodes, and NilB is a known host range determinant. Therefore, we considered host species as an environmental niche and bioinformatically examined whether the complement of DUF560 genes in a microbe corresponds with host phylogeny (37)(38)(39)(40)(41). All 46 Xenorhabdus genomes on the Magnifying Genome (MaGe) platform (42) encode between one and three TXISS, with five distinct homologs represented across the genus (one TXISS-1A and four TXISS-1B) (43). The one TXISS homolog that is conserved among all Xenorhabdus species is TXISS-1B HrpB. The genomic contexts of six representative homologs are shown in Fig. S4. Each unique combination of TXISS homologs was assigned one of six classes (A to F) ( Fig. S4 and Table S3). To visualize correlations between TXISS class and host identity, we constructed maximum likelihood and Bayesian phylogenetic trees for Xenorhabdus and Steinernema. Xenorhabdus trees were generated with whole-genome data, while Steinernema trees used five available loci ( Fig. S5 and Table S4). Aligning the maximum likelihood phylogenies reveals that the TXISS complement of a Xenorhabdus species is more predictive of nematode host than phylogenetic position (Fig. 6).
This alignment of the phylogenetic placement of a given Steinernema host with the TXISS complement of the symbiont provides insights into the nematode internal environment experienced by the symbiont. For example, Xenorhabdus with two HrpB paralogs at the hrp locus are symbionts of nematodes within the phylogenetic clade IV, suggesting that these nematodes present a distinctive environment in which a second hrp locus is beneficial. Xenorhabdus with an HrpB paralog encoded adjacent to genes predicted to encode a cobalt ABC transporter are solely symbionts of clade V nematodes. Xenorhabdus innexi and Xenorhabdus stockiae have seemingly diverged from this lineage through acquisition of a nilB homolog and switching to hosts within clade II. Similarly, X. nematophila independently gained nilB and switched into a clade II host (Fig. 6B). These acquisitions, alongside the varied genomic contexts of nilB/nilC pairs, are consistent with previous suggestions that the nil locus was horizontally acquired among Xenorhabdus (18).
The TXISS NilB and the lipoprotein NilC are necessary for X. nematophila to colonize the clade II nematode S. carpocapsae (14,18). However, X. nematophila also colonizes two nematodes, Steinernema anatoliense and Steinernema websteri, which are phylogenetically separate from clade II (Fig. 6). Our hypothesis that TXISS are involved in host-environment adaptations leads to the prediction that X. nematophila will require the nil locus to colonize these nematodes. To test this hypothesis, bacterium-free S. anatoliense, S. websteri, The Type 11 Secretion System ® and S. carpocapsae eggs (44) were exposed to an X. nematophila carpocapsae ATCC 19061 Dnil mutant and a nil-complemented strain (15). Consistent with our prediction, the Dnil mutant was deficient in infective juvenile colonization in all three nematode species (Fig. 7), demonstrating that nil genes are necessary for infective juvenile colonization of S. anatoliense and S. websteri and supporting our hypothesis that TXISS promote adaptation to host environments.

DISCUSSION
Bacteria have evolved specialized secretion systems for delivery of effectors that facilitate the host-associated lifestyle. Knowledge of cargo protein identities and sorting processes facilitates predictions from genomic information of bacterial secretome composition, regulation, and localization. Despite the diverse secretion systems now recognized, more remain to be identified based on the fact that some proteins predicted to be secreted lack known secretion pathways (45). The type X secretory pathway was described in 2020 shedding light on long-standing mysteries surrounding the dependence on a muramidase for secretion of typhoid toxin across the cell wall (7). Enabled by availability of genomic data from myriad environments and ever-improving bioinformatic visualization tools, we have presented data that the TXISS is a broadly distributed molecular vehicle for moving proteins across the Gram-negative outer membrane. Our network analysis has revealed functionally relevant TXISS clusters, with cluster 1 members having a conserved role in host-microbe interactions.
The DUF560 (domain of unknown function) family presence in animal-associated bacteria was first recognized when it was noted that the host colonization factor NilB has homologs in several human pathogens (14). This observation was strengthened by subsequent demonstration that Slam1 and Slam2 from these bacteria facilitate surface presentation of host metal acquisition proteins (8,9,14). Using the Steinernema-Xenorhabdus symbiosis, here we provide evidence that the composition of TXISS in a bacterial symbiont genome correlates with host organism. During the evolutionary history of Xenorhabdus, the gain or loss of TXISS loci correlated with host switching events (Fig. 6). These data indicate that TXISS activity contributes to bacterial adaptation to new host environments, which is particularly relevant given the varied distribution of TXISS homologs among human pathogens. For instance, our work enables categorization of TXISS among animal-associated Neisseria species, including the human pathogens Neisseria meningitidis and Neisseria gonorrhoeae (46). Neisseria strains can encode up to 6 TXISS paralogs. N. meningitidis MC58 has two functionally characterized TXISS, Slam1 and Slam2. Our network analysis indicates N. meningitidis encodes a third Slam, NMB1466/ NP_274965, that also falls within cluster 1A. N. gonorrhoeae TXISS are represented in more nodes than N. meningitidis, notably occupying 19.3% of nodes in subcluster 1B (Fig. 3). The cluster framework we present here indicates that Neisseria use TXISS to secrete both lipoproteins and soluble cargo and that variation in TXISS composition among Neisseria strains may be predictive of host or tissue association phenotypes.
While DUF560 proteins were originally thought to represent a mechanism for lipoprotein surface exposure (8), recent studies have expanded that functional range to include peripheral membrane proteins (11) and now soluble secreted proteins, establishing the function of DUF560 outer membrane proteins in secretion of varied substrates. We predict that further study will uncover even more chemically diverse cargo for the distinct classes of TXISS, revealed through our network analysis. Here we focused on a single cluster of a DUF560 sequence similarity network (SSN). The remaining nine clusters likely represent diverse subfamilies responsible for transporting asyet-unknown cargo. The lipoprotein cargo proteins for which structural conformations are known, share a C-terminal eight-stranded b-barrel (9). Our discovery that HrpC is a cargo protein for TXISS-1B HrpB strengthens the concept that this barrel is an important characteristic of TXISS cargo; HrpC is a homolog of the H. haemolyticus hemophilin, the structure of which likewise adopts a C-terminal eight-stranded b-barrel (29) (see Fig. S2 in the supplemental material). These data support the concept that TXISS cargo have bifunctional structures in which the N terminus is the host effector domain, while the C terminus targets secretion. This framework will facilitate identification of as-yet-unknown TXISS cargo among the genes that co-occur with TXISS outer membrane proteins. It is our hope that the DUF560-TbpBBD co-occurrence table used for our network annotation will become a resource for other microbiologists studying secreted proteins (see Table S2 in the supplemental material). This database identifies 851 potential TXISS/cargo pairs spread across 463 bacterial isolates, only 4 of which have been experimentally investigated thus far. Furthermore, our analyses suggest that SSN clusters have predictive power for other characteristics of TXISS cargo, including whether they are surface attached or secreted. The network-enabled classification FIG 7 The nil locus is necessary for colonization of S. anatoliense, S. carpocapsae, and S. websteri infective juveniles. Bacterium-free S. carpocapsae, S. anatoliense, and S. websteri were exposed to GFP-expressing X. nematophila lacking or bearing the nil locus. (A and B) The resulting progeny infective juveniles (IJs) were monitored for colonization either by microscopy (A) or by plating lysates for average CFU/IJ. (B) (C) The average CFU per colonized IJ combines both of these values to show bacterial load per organism. Treatments were analyzed via one-way ANOVA and Tukey's post hoc test.
The Type 11 Secretion System ® presented here will facilitate the investigation of both TXISS outer membrane proteins (OMPs) and their cargo in diverse bacteria.
Slam1 and Slam2 TXISS-dependent cargo with known molecular function include those that serve as coreceptors for TonB-dependent metal uptake systems (2). Similarly, HrpC, which we show here is a TXISS-dependent cargo protein, and its homologs also likely function in metal acquisition by acting as hemophores akin to hemophilin and HasA (29,47,48). This idea is supported by the fact that an N. gonorrhoeae hrpC homolog (NGO0554) is repressed by iron, upregulated in response to oxidative stress, and contributes to resistance to peroxide challenge (49)(50)(51). Further, the hrp locus, which is conserved across proteobacteria, is predicted to encode TonB and a TonB-dependent receptor (Fig. S4). Our working model is that the metal bound by secreted HrpC is passed to its respective TonB-dependent receptor and imported into the cell through TonB energization (2). Given the conservation of the TXISS hrp locus among all Xenorhabdus and throughout human microbiome constituents, it will be important in the future to examine the regulation of HrpC TXISS-dependent secretion and the roles of hrp machinery in binding and acquiring host metals in a mucosal environment.
In addition to their long-recognized roles in import, TonB and TonB-dependent receptors may also function in protein export. The Myxococcus xanthus TonB-dependent transporter Oar can export the protease PopC using the proton motive force to energize membrane translocation (52). Despite this expansion of the known functional range of TonB-dependent transporters, in the case of the TXISS clusters, TonB is likely responsible for energizing uptake systems, but not TXISS-mediated export. This suggestion is supported by the fact that both the TXISS-1A function of Slam-1 (8) and the TXISS-1B function of HrpB shown here could be reconstructed in E. coli without coexpression of native TonB. However, it will be interesting for future studies to investigate possible functional interactions between TonB-dependent transporter-mediated secretion and TXISS-mediated secretion.
Our initial network generated from the entire PF04575 (DUF560) data set included cluster 5, comprising 14 nodes of Klebsiella homologs and 2 nodes with Klebsiella and Escherichia homologs annotated as PgaA/HmsH. This cluster was removed based on its limited number of nodes, predicted topology differences relative to the rest of the network (16-stranded versus 14-stranded barrel), and the fact that the majority of known PgaA homologs were not represented within the cluster. PgaA is a component of one of several machineries for secretion of exopolysaccharides (EPSs) that comprise biofilm matrices of Gram-negative bacteria (53)(54)(55). Despite the topological and substrate (polysaccharide versus protein) differences, the PF04575 assignment of cluster 5 PgaA homologs hints that there could be evolutionary or structural parallels between exopolysaccharide synthase-dependent secretion systems and TXISS. For instance, both TXISS and EPS secretion machineries either have, or associate with proteins that have, tetratricopeptide repeat (TPR) domains (53). In EPS secretion systems, the TPR are necessary for secretion or for cargo modification (54,56). TXISS TPR domains may similarly modulate activities of other proteins that influence secretion of TXISS substrates. Support for a role of TPR domains in TXISS activity comes from the fact that X. nematophila expressing versions of the NilB TPR domain with small deletions display colonization defects that are ameliorated by deletion of the entire N-terminal periplasmic domain (15). Our establishment of TXISS as a bona fide secretion system opens new avenues for exploration of its integration with other export and secretion machineries and its coordinated contributions to host-associated phenotypes, including metal homeostasis, aggregation, and biofilm formation. Table S5 in the supplemental material. DUF560 SSN analysis. The EFI Enzyme Similarity Tool (EFI-EST) was used to collect all predicted DUF560 domain-containing sequences from Interpro 73 and UniProt 2019-02 (accessed 24 April 2019) and compare using BLAST (21,22,25). Representative networks collapsed nodes which shared $40% identity. On an EFI-EST network, edges are drawn according to a database-independent alignment score. Greater scores correspond to fewer edges. For separation of DUF560 clusters, an alignment score of 38 was chosen (Fig. 2). For subclusters 1A and 1B, 89 and 100 were chosen, respectively (see Fig. S1 in the supplemental material). Networks were visualized and interpreted using Cytoscape v3.7.1 (57) and Gephi v0.9.2 (58). Nodes were arranged with the Fruchterman-Reingold force-directed layout algorithm (59).

All primers and strains are listed in
The contents of each cluster were compared to Pfam DUF560 (PF04575) to ensure that clusters contained legitimate DUF560 proteins (26). Any clusters for which fewer than 18% of sequences were present in Pfam, or with fewer than 20 sequences, were excluded. This filtering removed cluster 5, composed mostly of Klebsiella pneumoniae PgaA. The resulting SSN contains 10 clusters, 1,222 nodes, and 52,190 edges with 1,589 TaxIDs represented (Fig. 2). Using NCBI Taxonomy Browser, each node was manually curated for the isolate's environmental origin(s) among the following categories: water, soil, plant, mammal, animal, invertebrate, nematode, built (environments such as sewage, bioreactors, etc.), multiple environments, and unclassified (Table S1). If no citation was available, the source was curated from BioSample or BioProject records or if neither was available, from other resources (NCBI linkout, Google search). Animal-associated microbes were designated as mammal, insect, nematode, or generic animal associated. Our fine-scale analysis focused on cluster 1 and its subclusters ( Fig. 3 and Fig. S1).
Three different techniques were used to determine whether DUF560 proteins within our network were genomically associated with TbpBBD domain-containing proteins. First, using EFI-GNN, genome-neighborhood-networks were generated for subcluster 1A (alignment score, 89; 40 total flanking ORFs, 20 upstream and 20 downstream of the DUF560), subcluster 1B (alignment score, 38; 20 total flanking ORFs, 10 upstream and 10 downstream of the DUF560), and subcluster 1C (alignment score, 38; 20 total flanking ORFs, 10 upstream and 10 downstream of the DUF560), resulting in 352 DUF560-TbpBBD pairs (22). Next, the RODEO web tool was used to analyze cooccurrence using profile hidden Markov models to assign domains to local ORFs resulting in 712 DUF560-TbpBBD pairs (27). Seven additional DUF560-TbpBBD pairs in Xenorhabdus were manually annotated. All three data sets were combined for a total of 851 nonredundant protein pairs (Table S2). SignalP-5 (60, 61) was used to predict the signal peptides of all TbpBBD domain-containing proteins and annotate each node (Fig. 3C). Any node associated with both signal peptide-bearing and signal peptide-free proteins was annotated according to those with a signal.
DUF560 genome neighborhood analysis. Subclusters 1A to C were separated and analyzed in EFI-EST with an alignment score of 38 as described above. Each network was then analyzed through EFI-GNN and visualized in Cytoscape v3.7.1 (57) (alignment score, 38; 10 ORFs up-and downstream).
Expression of HrpB and HrpC. The gene encoding HrpB25_26insDYKDDDDK (FLAG-HrpB) was synthesized and cloned into the second multiple cloning site (MCS) of pETDuet-1 by Genscript. The genomic region containing HrpC was amplified from the X. nematophila ATCC 19061 (HGB800) genome using primers 1 and 2, digested with restriction enzymes SacI and SalI, and ligated into the pUC19 MCS. Site-directed mutagenesis was used to add a C-terminal FLAG tag onto HrpC using primers 3 and 4. The gene encoding HrpC1_2insV246_247insDYKDDDDK (henceforth HrpC-FLAG) was amplified from pUC19 using primers 5 and 6. The product was ligated into MCS1 of both pETDuet-1 and pETDuet-1/FLAG-HrpB, resulting in expression plasmids pETDuet-1/HrpC-FLAG and pETDuet-1/HrpC-FLAG/FLAG-HrpB. HrpC-FLAG was used to create HrpC1_2insV246_247ins3x(GGGGS)-GST domain (HrpC-GST) using Hi-Fi assembly and primer pair 7 and 8 and primer pair 9 and 10 to integrate a 15-amino-acid linker and GST into the previous FLAG locus. This process yielded pETDuet-1/HrpC-GST and pETDuet-1/HrpC-GST/FLAG-HrpB. All clones were confirmed by Sanger sequencing using primers 11 and 14 at the Univeriity of Tennessee (UT) Genomics Core.
Expression plasmids were transformed into E. coli B21(DE3) and E. coli C43(DE3) via electroporation. Results were similar for the two strains. Strains were grown in defined medium with 150 mg/ml ampicillin (62). Bacteria were subcultured into 100 ml of broth at an initial optical density at 600 nm (OD 600 ) of 0.028, grown for 18 h at 37°C to reach late logarithmic growth, and induced with 500 mM isopropyl-b-D-thiogalactopyranoside (IPTG). After 1 h, 700 ml of each culture was filtered for subsequent use. At 2.5 h, whole cells were collected by centrifugation and lysed using a bead beater. Remaining supernatants were filtered. Protein concentration of whole-cell lysates was measured via Bradford assay. For supernatant samples, 700 ml of supernatant was precipitated via 10% trichloroacetic acid (TCA) (63).
Samples were analyzed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. For lysates, wells were loaded with 9.5 mg of protein. For supernatants, wells were loaded with half the TCA precipitate collected media. Western blots were probed with either rat anti-FLAG or rabbit anti-GST primary antibody anti-IgG secondary antibody that fluoresces at 680 nm. Intensities were recorded for FLAG or GST reactive bands which were the correct size for mature HrpB and HrpC. For every supernatant sample, a distinct band from the Coomassie bluestained gel was used as a loading control to normalize intensities of supernatant samples prior to analysis (Fig. S3). Unpaired t tests were used to compare HrpC-FLAG to HrpC-GST secretion and supernatant fraction HrpB. One replicate of the HrpC-GST 1-h supernatant was excluded from analysis For additional analysis of OMVs, bacteria producing each expression plasmid were subcultured in triplicate into 40 ml of broth at an initial OD 600 of 0.04. After 5 h at 37°C to reach mid-logarithmic growth, cultures were induced with 500 mM IPTG. After an additional 2.5 h, each culture was clarified via centrifugation, then the supernatant was filtered and centrifuged at 150,000 relative centrifugal force (RCF) for 3 h. The entire OMV pellet was solubilized in SDS sample buffer. Six hundred microliters of the total supernatant or the clarified (postultracentrifugation) supernatant were precipitated via 10% TCA and resuspended in SDS sample buffer (63). Samples were analyzed by SDS-PAGE and Western blotting as described above.
For nematode phylogenetic analysis, select Steinernema and Heterorhabditis species were analyzed. The internal transcribed spacer, 18S rRNA, 28S rRNA, cytochrome oxidase I, and 12S rRNA loci were collected from GenBank and used as homologous sets (Table S4). Nematode species with fewer than three of five loci sequenced were excluded. Homologous sets were processed using the same methods as the Xenorhabdus sequences, although for these sequences Modettes v2.1.10 (70) chose the GTR1g1I substitution model.
Maximum likelihood analyses were performed via RAxML v8.2.10 (70) using rapid bootstrapping and 1,000 replicates and were visualized via Dendroscope v3.6.2 (71). Nodes with less than 60% bootstrap support were collapsed. Bayesian analyses were performed via MrBayes v3.2.6 with BEAGLE (72-74) on the CIPRES Science Gateway platform (75). A total of 500,000 or 4,000,000 Markov chain Monte Carlo (MCMC) replicates were performed for the bacterial or nematode tree, respectively. Twenty-five percent were discarded as burn-in, and posterior probabilities were sampled every 500 replicates. Two runs were performed with three heated chains and one cold chain. The final standard deviation of split frequencies was less than 10 26 for the bacterial tree, and it was 0.002557 for the nematode tree. Bayesian trees were visualized with FigTree v1.4.4 (76). Bayesian and maximum likelihood methods generated phylogenies with consistent topologies (Fig. 6 and Fig. S5).
Wild type versus Dnil colonization of nematodes. Bacterium-free eggs of S. anatoliense, S. carpocapsae, and S. websteri (44) exposed to a green fluorescent protein (GFP)-expressing Nil mutant (HGB1495) or its complemented strain (HGB1496) were grown on lipid agar plates for 2 days at 25°C (15). Plates were placed into White traps 1 week after plating to collect infective juvenile (IJ) nematodes. Nematode colonization was visualized using fluorescence microscopy on a Keyence BZX-700 to observe bacteria in the receptacle (in biological triplicate and technical duplicate). To determine the number of CFU per IJ, nematodes were surface sterilized and ground for 2 min using a Fisher brand motorized tissue grinder (catalog no. 12-1413-61) to homogenize the nematodes and release colonizing bacteria. Serial dilutions in phosphate-buffered saline (PBS) were plated on LB agar and then incubated at 30°C for 1 day before enumerating CFU (Fig. 7). To calculate the CFU per colonized IJ, the percent colonized nematodes was divided by the CFU/IJ for each biological replicate. The data were analyzed using a oneway analysis of variance (ANOVA) with Tukey's multiple-comparison test to compare the mean of each treatment.

SUPPLEMENTAL MATERIAL
Supplemental material is available online only.   We have no competing interests that might be perceived to influence the results and/or discussion reported in this paper.