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Fibronectin in saliva and gingival crevices.

The aims of this study were to determine if fibronectin is present in gingival crevices and in unstimulated or stimulated whole, parotid, and submandibular/sublingual salivas. Eight subjects with healthy gingiva were selected. Two identical examinations with an interval of at least 2 weeks were performed. The examinations included collection of crevicular material and different salivas, with secretion rates recorded. Blood samples were taken and the concentration of plasma fibronectin was used as a positive control. The presence of fibronectin was studied by an ELISA assay. Fibronectin was found in whole saliva and in submandibular/sublingual saliva but could not be demonstrated in parotid saliva. Fibronectin was also found in crevicular material and in plasma. There was no statistically significant difference between the concentration of Fibronectin per μg protein in plasma compared to crevicular material. The amount of fibronectin, expressed as μg/ml. of the different salivas varied between the two examinations and the largest concentration was most often found in unstimulated whole saliva. When the secretion rate was taken into consideration, there were variations between the two examinations and the largest output of fibronectin over time was seen in stimulated whole saliva.