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Research on the Inhibitory Activity of Endophytic Fungi in Huperzia serrata against AchE

Twenty-two endophytic fungi in the Huperzia serrata were separated to test their fermentation broth. Research found that seven of them could produce alkaloid. Of these, the fermentation products from the T4 bacterial strain produced the most, with 2.027 mg/g. Ellman colorimetry has determined that the fermentation broth in four fungi had inhibition activity against AchE. It also showed that the total alkaloid of the T17 bacterial strain had a half-inhibitory concentration (IC50) of 98.494 μg•mL-1 on the mouse’s brain against AchE. This yielded the conclusion that the total alkaloid in the T17 fermentation broth restrains AchE effectively, and that it further contributes to the separation of its active ingredient.