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A Reverse Phase Liquid Chromatography Analysis of Citicoline Sodium in Pharmaceutical Dosage Form using Internal Standard Method

In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the determination of Citicoline sodium in pharmaceutical dosage form. Chromatographic separation was carried out on a RP-18 column using a mobile phase consisting of acetonitrile: water (20:80, v/v) adjusted at pH 3.0 using 1% orthophosphoric acid. Flow rate was maintained at 0.7ml min and UV detection was carried at 260 nm. Caffeine was used as an internal standard. The calibration curve was found to be linear over the range 1–500μgml. The results of accuracy study were ranged between 99.32% and 101.90% with a R.S.D. less than 1%. LOD and LOQ were 1.4μgml and 4.3μgml, respectively. The method was found to be simple, rapid, and easy to apply, making it very suitable for routine analysis of Citicoline sodium in pharmaceutical dosage form.