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Construction and comparison of recombinant plasmids encoding type 1 fimbriae of members of the family Enterobacteriaceae

The genes encoding type 1 fimbriae of Salmonella typhimurium, Enterobacter cloacae, and Serratia marcescens were cloned in Escherichia coli. All transformants possessing recombinant plasmids were shown to be fimbriate and demonstrated mannose-sensitive hemagglutinating activity. A comparison of the physical maps of these plasmids revealed little similarity among them, although plasmids encoding type 1 fimbriae of Escherichia coli and Klebsiella pneumoniae appeared similar with respect to restriction enzyme sites. The fimbrial gene cluster ranged in size from 5.5 to 9.0 kilobase pairs as determined by transposon mutagenesis. Plasmid-containing E. coli strains were shown to produce species-specific fimbrial antigens with little or no cross-reactivity between genera. Therefore, it was presumed that each plasmid contained the gene encoding the fimbrial subunit. Complementation was not detected between nonfimbriate insertion mutants of different species but was seen with mutants of the same species.