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[Determination of isoguanosine in Semen Crotonis Tiglii by high-performance liquid chromatography].

OBJECTIVE
To develop a high-performance liquid chromatographic method for the determination of isoguanosine in Semen Crotonis Tiglii.


METHODS
The determination was done by using reverse-phase high-performance liquid chromatography with a Hedera ODS-2 column (4.6 mm x 250 mm, 5 microm). Elution was employed with the mobile phase of acetonitrile-methanol-water (1:4:95) at flow rate of 1.0 mL/min. The detection wavelength was set at 292 nm and the column temperature was 25 degrees centigrade. Injection volume was 10 microL.


RESULTS
The linear range of isoguanosine was from 0.161 to 0.967 mg. The correlation coefficient of the calibration curves was 0.999 8. The average recovery rate was 98.78% with relative standard deviation of 0.02%.


CONCLUSION
The results show that the method is simple, accurate, and repeatable, and it is suitable for quality control of Semen Crotonis Tiglii.