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A novel RT‐PCR method for the quantitative analysis of microRNA expression: miR‐1/124 expression in mouse CNS

More than 700 microRNAs have been cloned, and the functions of these molecules in developmental timing, cell proliferation, and cancer have been investigated widely. In this report, we describe a novel RT‐PCR method for the quantitative analysis of microRNA expression. The use of this method in a model study revealed differential expression of miR‐1 and ‐124 in mouse organs. Specifically, the new method revealed that miR‐124 concentrations in the mouse CNS (central nervous system) were more than 100 times those in other organs. By contrast, miR‐1 expression in the CNS was 100 – 1,000 times lower than that in skeletal muscle and heart. Moreover, the new method revealed regional differences in miR‐124 expression within the CNS: expression ratios versus the cerebral cortex were 60.7% for the cerebellum and 35.4% for the spinal cord. These results suggest that our method would be a powerful new tool for studies of microRNA expression.