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Rapid lysis of cell walls of Micrococcus radiodurans with lysozyme: effects of butanol pretreatment on DNA.

A method is presented by which the walls of Micrococcus radiodurans are rendered sensitive to rapid decomposition by lysozyme after a preliminary extraction with an n-butanol-saturated buffer. The method has been adapted for density gradient centrifugation studies of DNA in alkaline sucrose.Butanol induced an endonuclease activity which was inhibited by EDTA. Endonuclease-induced breaks were not repaired. Protoplasts did not repair X-ray-induced breaks. Therefore, pretreatment with butanol inactivated the DNA repair system. Untreated cells repaired a portion of their X-ray-induced breaks.