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Mastitis incidence in straw yards and cubicles

infected quarters were odourless and varied from a brown coloured milk to a purulent discharge. In longer established cases, gradual separation of milk samples into a sediment and a serumlike supernatant was the most consistently observed feature. All cows were intensively treated with broad-spectrum antibiotics, with minimal effect on the clinical signs. No pathogenic bacteria or yeast were isolated from initial milk samples. M bovis was detected by a monoclonal antibody-based sandwich ELISA (Ball and others 1994), and subsequently cultured from milk samples collected from the mastitic cows three weeks after the outbreak began. One of the imported Montbelliardes was also diagnosed as having M bovis mastitis shortly after the first seven cases. These eight cows and two cows suspected on clinical signs were segregated and milked after the rest of the herd. Nine weeks after the outbreak began, quarter milk samples were collected aseptically from all 44 lactating cows. M bovis was detected in milk samples from 10 clinically infected quarters and from a further 11 subclinically infected quarters all from the eight previously identified cows. Milk sccs ranged from 1,752,000 to 21,381,000 cells/ml in samples collected from quarters with subclinical M bovis infection. Four cows developed infection in all four quarters and were dried off. Three cows resumed production of apparently normal milk but subclinical M bovis mastitis was later observed in two of these cows. Another infected cow continued to produce a sediment in mammary secretions from two infected quarters. Segregation of affected cows, which included milking them last, was followed by a reduced incidence of M bovis mastitis in this herd. During the following three months only four new cases were identified on this farm and these included the two suspect