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Citrate-Buffered Methylene Violet Stain as an Alternative to Conventional Stains Used to Determine Yeast Viability

1. Overestimation of ale and lager yeast viabilities by greater than 10% occurred 71.4 and 48.2% of the time, respectively, and underestimation of ale and lager yeast viabilities occurred 1.8 and 12.5% of the time, respectively, by the methylene violet stain method. 2. Overestimation of ale and lager yeast viabilities occurred 53.6 and 44.6% of the time, respectively, and underestimation of the ale and lager yeast viabilities occurred 12.5 and 16.1% of the time, respectively, by the methylene blue stain method. 3. In this year’s ruggedness testing, reliable conditions for consistent viability measurements between labs, as determined by the slide culture method, were not attained. 4. Citrate buffered methylene violet provided no benefit over methylene blue in the determination of yeast viability.