{'fraction_non_alphanumeric': 0.011342155009451797, 'fraction_numerical': 0.0, 'mean_word_length': 5.418181818181818, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Effects of neonatal calf serum on differentiation of human fetal neural stem cells in the hippocampus].

OBJECTIVE
To investigate the effects of neonatal calf serum on the differentiation of human neural stem cells in the hippocampus.


METHODS
The effects of various concentrations of neonatal calf serum on the differentiation of human fetal neural stem cells in the hippocampus were observed in cell culture experiment and immunocytochemical staining.


RESULTS
Neonatal calf serum efficiently induced the differentiation of human fetal neural stem cells into neurons and astrocytes, whose amount varied between serum-free cell culture and the culture with neonatal calf serum of different concentrations. As the concentration of neonatal calf serum increased, the differentiation of human fetal neural stem cells shifted toward astrocytes, while the differentiation into neurons was decreased.


CONCLUSION
Neonatal calf serum causes alteration of the ratio between neurons and glial cells differentiated from human neural stem cells in the hippocampus.