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EFECTIVIDAD DE Candida sake INCORPORADA A RECUBRIMIENTOS BIODEGRADABLES EN EL CONTROL DE Botrytis cinerea EN UVA

The effectiveness improvement of Candida sake as biocontrol agent (BCA) against Botrytis cinerea on grapes was studied by applying edible coatings (EC) obtained with different proportions of coating forming solids versus the BCA. Coating forming dispersions were prepared with two biopolymers: pregelatinized potato starch (AM) and sodium caseinate (NaC). Two types of controls, samples treated with water and other with C. sake dispersed in an aqueous solution (CS), were used. The solids concentrations of these dispersions were 2.50, 3.75 and 5.00 mg of coating forming solids ⋅mL (w/v), respect to a fixed concentration of antagonist of 5⋅10 CFU mL. Each dispersion with BCA was sprayed on grapes and after drying, population dynamics at 24 h and 7 days of controlled storage were determined at 20oC and 85%HR. In another serie of samples, the efficacy of these treatments (EC with BCA) to control B. cinerea was studied. To that end, when EC were dried, a B. cinerea suspension of 10 conidia mL was applied. Then, the samples were incubated under the same conditions of temperature and relative humidity for 7 and 9 days, and a visual examination of the affected fruit surface was conducted to determine the incidence and severity of the infection. Results were expressed as reduction of the incidence and severity, referred to control samples treated with water. The