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[Developing of a new multicolor-fluorescent labeled STR amplification kit].

OBJECTIVE
To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253).


METHODS
By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci.


RESULTS
Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit.


CONCLUSION
The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.