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Suppressing Cyanobacteria with Hydrogen Peroxide Is More Effective at High Light Intensities

Hydrogen peroxide (H2O2) can be used as an emergency method to selectively suppress cyanobacterial blooms in lakes and drinking water reservoirs. However, it is largely unknown how environmental parameters alter the effectiveness of H2O2 treatments. In this study, the toxic cyanobacterial strain Microcystis aeruginosa PCC 7806 was treated with a range of H2O2 concentrations (0 to 10 mg/L), while being exposed to different light intensities and light colors. H2O2 treatments caused a stronger decline of the photosynthetic yield in high light than in low light or in the dark, and also a stronger decline in orange than in blue light. Our results are consistent with the hypothesis that H2O2 causes major damage at photosystem II (PSII) and interferes with PSII repair, which makes cells more sensitive to photoinhibition. Furthermore, H2O2 treatments caused a decrease in cell size and an increase in extracellular microcystin concentrations, indicative of leakage from disrupted cells. Our findings imply that even low H2O2 concentrations of 1–2 mg/L can be highly effective, if cyanobacteria are exposed to high light intensities. We therefore recommend performing lake treatments during sunny days, when a low H2O2 dosage is sufficient to suppress cyanobacteria, and may help to minimize impacts on non-target organisms.


Introduction
Cyanobacteria can develop dense blooms in eutrophic lakes and reservoirs [1,2]. Many of the bloom-forming cyanobacteria, such as Microcystis aeruginosa, can produce secondary metabolites that are toxic to plants, invertebrates and vertebrates, including birds and mammals [3][4][5]. Toxic cyanobacteria can also be a human health risk, especially in bathing waters or drinking water supplies [6][7][8].
Consequently, toxic cyanobacterial blooms may interfere with the intake of water by drinking water companies, and lead to the closure of recreational lakes, sometimes with large socioeconomic consequences [9][10][11][12]. In view of the World Health Organization (WHO) guideline value for safe drinking water, it is essential to keep the concentrations of cyanotoxins such as microcystin low [4].
Eutrophication is one of the main driving forces behind the observed increase in cyanobacterial blooms worldwide [1,2,13,14]. Hence, the most sustainable solution to prevent bloom formation of toxic cyanobacteria is a reduction of nutrient inputs, particularly phosphorus (P) and nitrogen (N), into lakes In this study, we assess how light intensity and light color influence the effectiveness of H 2 O 2 against the microcystin-producing cyanobacterium Microcystis aeruginosa PCC 7806 (further named Microcystis). For this purpose, dense Microcystis cultures were pre-grown in laboratory chemostats provided with a nutrient-rich mineral medium and kept in steady state. Subsequently, samples from these chemostats were treated with a range of H 2 O 2 concentrations exposed to different light intensities and light colors. Lakes with cyanobacterial blooms are often quite turbid (hazy). Therefore, we applied light intensities ranging from 0 to 150 µmol photons·m −2 ·s −1 as representative light conditions for the top 1-2 m of turbid lakes dominated by cyanobacterial blooms [63,64]. The effectiveness of the H 2 O 2 treatments was evaluated by monitoring several physiological variables, including photosynthetic yield, MC concentrations and cell size. The results provide insight into how H 2 O 2 damages cyanobacterial cells, and they also help to elucidate which light conditions are most suitable for the H 2 O 2 treatment of cyanobacterial blooms in lakes.

Photosynthetic Vitality
The photosynthetic vitality of Microcystis, defined as the photosynthetic yield as percentage of the control treatment, was reduced by the H 2 O 2 treatments (Figure 1A-E; Table S1). The decrease in photosynthetic vitality was accompanied by an increase of F 0 , followed by an increase of F m after the first 1-2 h ( Figure S1).
The decline of the photosynthetic vitality was strongly dependent on both the H 2 O 2 dosage and the light intensity. H 2 O 2 treatments in the dark had the smallest effect, whereas increasing light intensities strengthened the effect of H 2 O 2 on the photosynthetic vitality. For example, an addition of 10 mg/L H 2 O 2 in the dark caused only a 30% decline in photosynthetic vitality ( Figure 1E), whereas the addition of 1 mg/L H 2 O 2 at a light intensity of 150 µmol photons·m −2 ·s −1 caused a complete decline of the photosynthetic vitality to zero within 2.5 h ( Figure 1A).
Dose-response models of the photosynthetic vitality after 4 h were fitted as function of the H 2 O 2 concentration using nonlinear regression ( Figure 2). The parameter estimates of the dose-response models are reported in Table S2. Pairwise comparison of the dose-response models using the Akaike Information Criterion corrected for small sample sizes (AICc) showed that the dose-response models differed between the different light intensities (Table S2). In other words, light intensity affected the H 2 O 2 sensitivity of Microcystis. The estimated EC 50 values ranged from >10 mg/L in darkness to <1 mg/L at a light intensity of 150 µmol photons·m −2 ·s −1 (Figure 2; Table S2).

H 2 O 2 Degradation
H 2 O 2 was slowly degraded during the first 4 h of the experiment, irrespective of light intensity ( Figure 1F-J). Degradation rates varied only slightly between the different H 2 O 2 concentrations. In the treatment with 10 mg/L of H 2 O 2 in the presence of Microcystis, the H 2 O 2 concentration had declined to 7.41 ± 0.50 mg/L (mean ± standard deviation (SD); n = 10) after 4 h ( Figure 1J). For comparison, in the controls without Microcystis that were provided with 10 mg/L of H 2 O 2 , the H 2 O 2 concentration declined only marginally, to 9.92 ± 0.28 mg/L (n = 10) and 9.74 ± 0.26 mg/L (n = 10) after 4 h in Milli-Q water and BG-11 medium, respectively. Hence, H 2 O 2 was degraded significantly faster in the presence than in the absence of Microcystis cells (Student's t-test of treatment vs control in BG-11 medium: t = 20.3, df = 9, p < 0.001).

Microcystin Concentrations
The total MC concentration, as well as the proportion of MC in the cells (intracellular MC), and in the water phase (extracellular MC), was strongly affected by both the H 2 O 2 dosage and light intensity ( Figure 3). After 24 h exposure to H 2 O 2 in the dark, the intracellular MC of Microcystis cells had decreased only slightly, and the extracellular MC concentration was only a minor fraction of the total MC concentration, even at the highest H 2 O 2 dosage of 10 mg/L ( Figure 3A). The intracellular MC had increased in Microcystis cells treated with 0 and 1 mg/L of H 2 O 2 at 15 µmol photons·m −2 ·s −1 ( Figure 3B). Most likely, oxidative stress at this low light level and H 2 O 2 dosage was minimal, which enabled cell growth, and hence an increase of the MC concentration during the 24-h incubation. In contrast, hardly any intracellular MC could be detected after 24 h, and most MC ended up in the extracellular MC pool when Microcystis cells were treated with ≥4 mg/L H 2 O 2 at 15 µmol photons·m −2 ·s −1 ( Figure 3B), or when they were treated with ≥2 mg/L H 2 O 2 at 100 µmol photons·m −2 ·s −1 ( Figure 3C).
In those H 2 O 2 treatments where the intracellular MC disappeared, the extracellular MC concentration measured after 24 h of H 2 O 2 exposure did not reach the total MC concentration of the Microcystis cultures prior to H 2 O 2 exposure. This indicates that part of the MC was lost or not recovered by our analysis.
As a control, we exposed pure MC-LR in BG-11 medium, but without Microcystis cells to the different H 2 O 2 and light treatments. The results showed that pure MC-LR was hardly degraded by H 2 O 2 irrespective of the light conditions; the MC-LR concentration declined by, on average, less than 1.5% after 24 h ( Figure S2).

Biovolume, Cell Size and Cell Counts
Cell numbers increased during the 24-h time span of our experiments when Microcystis was grown in the light at 0 and 1 mg/L H 2 O 2 , whereas cell numbers remained constant for all H 2 O 2 treatments in the dark and for cells exposed to ≥2 mg/L H 2 O 2 in the light ( Figure 4A-C). The constant cell numbers in the treatments exposed to ≥2 mg/L H 2 O 2 indicate that these cells had ceased growth, but did not disintegrate during the 24-h experiments of this study, as confirmed by a microscopic inspection of the samples. Interestingly, the diameter of Microcystis cells was slightly reduced when they were exposed to ≥2mg/L H 2 O 2 in the light ( Figure 4D-F). As a consequence of the observed changes in cell numbers and cell size, the total biovolume of Microcystis grown in the light was much higher at 0 and 1 mg/L H 2 O 2 than at ≥2 mg/L H 2 O 2 ( Figure 4G-I).

Light Enhances Effectiveness of H 2 O 2
Suppressing cyanobacteria in lakes with H 2 O 2 should be done with a concentration that is effective against cyanobacteria, but minimizes harmful effects to nontarget organisms such as zooplankton [27]. Our study shows that environmental conditions can influence the effectiveness of H 2 O 2 treatments. Specifically, our results demonstrate that the H 2 O 2 effectiveness against cyanobacteria is drastically enhanced at high light intensities. Even a very low H 2 O 2 concentration of 1 mg/L, which hardly showed any effect at darkness or low light, was fatal to Microcystis under high light conditions. Consequently, days of long and intense sunshine should be selected to enhance the efficiency when treating cyanobacteria in lakes.
Possibly, preconditioning to high light may improve the defense of cyanobacteria against oxidative stress, and thereby may have an effect on the sensitivity of Microcystis to H 2 O 2 . For instance, previous exposure to high light influenced the response of the marine cyanobacterium Synechococcus sp. WH7803 to oxidative stress [65]. Furthermore, experiments with Microcystis PCC 7806 have shown that exposure to oxidative stress leads to a binding of microcystins to proteins to protect them against oxidative stress [66], and triggers the production of anti-oxidant enzymes such as peroxiredoxins and peroxidases [48].
However, the Microcystis cultures of our study were pre-grown at relatively low light intensities (~15 µmol photons·m −2 ·s −1 ), yet the observed sensitivity to H 2 O 2 was similar as in Mikula et al. [58] and Lürling et al. [67], who cultured Microcystis at higher light intensities of 140 µmol photons·m −2 ·s −1 and 100 µmol photons·m −2 ·s −1 , respectively. Moreover, Drábková et al. [46] specifically tested if pre-conditioning to high light intensities of the same Microcystis strain as in our study influenced its H 2 O 2 sensitivity, but then concluded that this was not the case.

Damage Caused by H 2 O 2
Why is H 2 O 2 more effective against cyanobacteria at high light intensities? How H 2 O 2 damages cyanobacterial cells is not yet completely understood. It is known that H 2 O 2 damages the D1 protein [68]. The D1 protein is part of the reaction center of photosystem II (PSII), and is in direct contact with the oxygen-evolving manganese cluster. The D1 protein and PSII are commonly damaged by light absorption, even in the absence of reactive oxygen species (ROSs) [69]. This damage can be remedied because the D1 protein has a very high turnover rate, and under modest light conditions the repair of PSII is faster than the damage rate, so that photoinhibition can usually be avoided [65,70]. However, H 2 O 2 may cause aggregation of the D1 proteins [68] and it also disrupts the D1 repair mechanism [71][72][73], which makes cells more sensitive to photoinhibition. Moreover, this interactive effect between H 2 O 2 and photoinhibition will be stronger at high light intensities, in agreement with our observations. Similar to previous studies, the effectiveness of H 2 O 2 addition was assessed by measurements of the photosynthetic yield using PAM fluorometry (e.g., [27,45,46,58]). The photosynthetic yield reflects the efficiency of photosynthetic electron transport at PSII, and hence will be very sensitive to the damage of PSII and the D1 protein induced by H 2 O 2 . The photosynthetic yield is derived from two fluorescence values, F 0 and F m [74,75]. F 0 is the minimum fluorescence of a dark-adapted culture, which can be interpreted as the background fluorescence when the reaction centers of PSII are "open". F m is the maximum fluorescence measured after a saturating light pulse when all reaction centers of PSII are "closed", and thus, no further electrons can be accepted and all additional energy is dissipated as heat and fluorescence [75]. Accordingly, an increase in damaged D1 proteins is expected to reduce the amount of open reaction centers, which will increase F 0 and consequently will reduce the photosynthetic yield. This was indeed observed in our study ( Figure S1).
Our observation that H 2 O 2 had a stronger effect on the photosynthetic yield in orange light (630 nm) than in the more energetic blue light (450 nm) indicates that the damaging effect of H 2 O 2 is linked to the absorption of light by different photosynthetic pigments. While Chl a and carotenoids are responsible for absorption of blue light, the chromophore phycocyanobilin in the phycobilisomes of Microcystis absorbs orange light. Luimstra et al. [62,76] investigated the effect of orange and blue light on cyanobacterial photosynthesis, and found that orange light leads to much higher rates of oxygen production by PSII than blue light. They hypothesized that the reason for this difference in oxygen evolution rates is caused by the way orange and blue light are distributed over PSI and PSII. More specifically, cyanobacteria have much more of their Chl a in PSI than in PSII, and hence, blue light is mostly transferred to PSI [62,[77][78][79]. Conversely, most phycobilisomes are attached to PSII under normal growth conditions (e.g., [62,80]), and hence the absorbed orange light is mostly transferred to PSII. As a result, the relative amount of light energy that arrives at PSII, where H 2 O 2 damages the D1 protein, is highest in orange light and lowest in blue light.
In addition to the effects of H 2 O 2 on photosynthesis, H 2 O 2 and other reactive oxygen species (ROSs) may also cause further cellular damage [30,81]. Our H 2 O 2 treatments caused bleaching of Microcystis, but did not result in a complete disintegration of the cells, as cell counts were stable throughout the 24-h experiments of this study, as well as in the 120-h experiments of Schuurmans et al. [48]. However, cell sizes of Microcystis and therefore also the total cyanobacterial biovolume were negatively affected by the H 2 O 2 treatments. The decrease in cell size is likely caused by a leakage of the cellular contents.
Several studies found that cells open and die upon H 2 O 2 exposure, as shown by SYTOX staining of cells with damaged cell membranes [58], electron microscopy [68,82,83] and measurement of K + release [83], as well as caspase-3-like activity as a trigger for programmed cell death [82,83]. Perforation of the cell membrane would also explain the release of intracellular MC, as observed in our study and several previous studies [67,84,85].

Microcystin Dynamics
The decrease of intracellular MC induced by the H 2 O 2 treatments of ≥2 mg/L H 2 O 2 at 15 and 100 µmol photons·m −2 ·s −1 was accompanied by a parallel increase of extracellular MC (Figure 3). Yet, the total MC concentration (i.e., the sum of intracellular and extracellular MC) prior to H 2 O 2 addition could not be recovered within 24 h after H 2 O 2 addition, a phenomenon also observed by Lürling et al. [67] and Schuurmans et al. [48]. The disappearance of MC cannot be attributed to H 2 O 2 -mediated degradation of MC, since pure MC in BG-11 medium was hardly degraded by H 2 O 2 ( Figure S2). However, it is known that MC binds to proteins when cells are exposed to oxidative stress, for instance, by H 2 O 2 addition [66]. Protein-bound MC is not methanol extractable, and hence will not be detected by the standard methods of MC analysis [86]. In other words, most likely, some part of the MC was temporarily bound to cellular proteins in response to the oxidative stress induced by H 2 O 2 [66], and therefore was no longer visible in our analysis. Schuurmans et al. [48] showed that 120 h after H 2 O 2 addition, the total extracellular MC concentration approached the total intracellular MC at the start of the experiments, which indicates that the protein-bound MC was gradually released in the mineral medium after further disintegration of the cells.
The fate of released MC is of relevance for the application of H 2 O 2 treatments in lakes and drinking water reservoirs. The absence (or near absence) of bacteria capable of the microbial degradation of MC explains why extracellular MC concentrations remained relatively high after H 2 O 2 addition in our laboratory experiments. In contrast, aquatic microbial communities of lakes and sediments can break down MC released by cyanobacterial blooms [87][88][89][90]. While new pathways for bacterial MC degradation are still being discovered [91][92][93], recent studies have shown that complete MC degradation in the field can occur within a few days [94][95][96]. This is consistent with the lake treatment of Matthijs et al. [27], where MC disappeared in 1-2 days after H 2 O 2 addition. In addition, H 2 O 2 treatments supplemented with the MC-degrading enzyme Mlr-A can further enhance the degradation of MC released by lysing cyanobacteria [97]. Whether interactions between H 2 O 2 and high light intensities affect this microbial degradation of MC is an interesting open question that may deserve further attention in future studies.

Implications for Lake Treatments
Our results show that light enhances the toxicity of H 2 O 2 for cyanobacteria, as H 2 O 2 -treated cells exposed to higher light intensities have lower photosynthetic yields, shrink in size, and release MC. One might argue that light intensities in our laboratory experiments (≤150 µmol photons·m −2 ·s −1 ) were much lower than the incident sunlight at the surface of lakes during sunny days (up to 2000 µmol photons·m −2 ·s −1 ; e.g., [64]). However, light intensities at the lake surface can be lowered by~90% due to cloud cover, and decrease rapidly with depth depending on the transparency of the lake [98]. Bloom-forming cyanobacteria often become dominant in relatively turbid lakes, and cyanobacterial blooms themselves even further reduce the transparency, such that light intensities ≤150 µmol photons·m −2 ·s −1 are reached within the first 1-2 m, and sometimes within the first few decimeters, of the water column even during sunny days (e.g., [63,64]). Hence, the range of light intensities applied in our study are highly relevant for many cyanobacterial blooms.
In stagnant waters under calm weather conditions, however, buoyant cyanobacteria such as Microcystis spp. may float upwards to the surface and form dense surface scums [2,99]. In this case, the top layer of the scum will be exposed to full sunlight and may suffer from severe photoinhibition [100].
Extrapolation of our results suggests that interference of H 2 O 2 with the D1 repair mechanism is expected to lead to an even stronger suppression of the PSII activity at these high light intensities. This is supported by studies at Lake Taihu and Lake Dianchi, in China [101,102], where these interactive effects between H 2 O 2 and light intensity were observed in samples taken from cyanobacterial blooms. In these studies, the samples were transferred to beaker glasses, exposed to H 2 O 2 and placed in full sunlight, which resulted in a much stronger reduction of the photosynthetic yield, compared to control samples that were placed in the dark or shaded.
Light intensity did not have a major effect on the degradation rate of H 2 O 2 in our laboratory experiments. However, degradation of H 2 O 2 in lakes is usually much faster than the low H 2 O 2 degradation rates in our axenic Microcystis cultures [27]. In lakes, anti-ROS enzymes of the microbial community, including bacteria as well as eukaryotic phytoplankton, and oxidation-reduction reactions with organic matter and metal ions are largely responsible for the degradation of H 2 O 2 [38,41,45]. Furthermore, photochemical production of H 2 O 2 and H 2 O 2 -mediated photodegradation of organic matter are known to have major impact on the H 2 O 2 dynamics in lakes [38,40]. Hence, contrary to our lab experiments, light intensity may affect H 2 O 2 degradation in lakes, which may influence the exposure time of cyanobacteria to the H 2 O 2 treatment.
Our results show that light color has an effect as well, as Microcystis was more sensitive to H 2 O 2 in orange than in blue light. These results may imply that it can be difficult to effectively suppress an upcoming cyanobacterial bloom in clear, blue lakes. In most cases, however, cyanobacterial blooms occur in more turbid lakes, where green or orange-red light often dominates the underwater light field [61]. In particular, lakes with relatively high concentrations of dissolved organic carbon (DOC) tend to be predominated by orange wavelengths that are effectively captured by the phycocyanobilin-pigments of bloom-forming cyanobacteria [62,103]. For example, Stomp et al. [104] reports that Lake Groote Moost, a turbid peat lake with high DOC concentrations, is characterized by an orange-red underwater spectrum, and is often dominated by cyanobacterial blooms. Although the high turbidity of these peat lakes reduces the overall light intensity, the remaining orange underwater color may still provide a suitable light spectrum to effectively suppress cyanobacterial blooms with H 2 O 2 .
Extrapolation of our laboratory results to lake conditions can help optimizing H 2 O 2 treatments of cyanobacterial blooms. Our results imply that lake treatments would require less H 2 O 2 on sunny days than on cloudy days. Similarly, lake treatments will be much more effective when H 2 O 2 is administered during daytime than in the evening or at night. Furthermore, depending on the transparency of the water column, a surface bloom could be treated with a lower H 2 O 2 concentration than a bloom located deeper in the water column or near the thermocline. Treating cyanobacterial blooms with H 2 O 2 under the most effective light conditions may help to reduce the dosage, and hence the cost of the treatment, and may diminish potential side effects on other organisms.

Chemostat Cultures
The axenic strain Microcystis aeruginosa strain PCC 7806 was kindly provided by the Pasteur Culture Collection (Paris, France). Microcystis was pre-cultured in four chemostats specially designed for phytoplankton growth [105] to ensure that all Microcystis experienced the same growth conditions prior to the hydrogen peroxide (H 2 O 2 ) experiments. Once the chemostats reached steady state, samples from the chemostats were transferred to batch culture experiments with H 2 O 2 additions as described below.
Each chemostat consisted of a flat culture vessel that was illuminated from one side by white fluorescent tubes (Philips Master TL-D 90 De Luxe 18 W/965; Philips Lighting, Eindhoven, The Netherlands). The incident irradiance (I in ) was set at 15 µmol photons·m −2 ·s −1 measured with a LI-COR LI-250 quantum photometer (LI-COR Biosciences, Lincoln, NE, USA). Microcystis was cultured in the nutrient-rich BG-11 medium [106] with 10 mmol/L NaNO 3 . The dilution rate of the chemostats was set at 0.01 h −1 .
The chemostats had an optical path length of 5 cm and an effective working volume of approximately 1.8 L. The temperature in the chemostats was maintained at 25 • C using stainless steel cooling fingers connected to two water baths (Haake A28F/AC200; Thermo Fisher Scientific, Pittsburgh, PA, USA). This temperature is representative for surface waters dominated by cyanobacterial blooms during warm summers at our latitude [64,107]. The chemostats were aerated with pressurized air containing 400 ppm CO 2 at a flow rate of 30 L/h, as described by Sandrini et al. [108].
Regular microscopical observations of chemostat samples did not reveal contamination with other photosynthetic organisms. Although the chemostats were started with axenic cultures of Microcystis, we could not prevent contamination by a few heterotrophic bacteria during the chemostat experiments. At the end of the experiments, the number of heterotrophic bacteria in the samples was <<5% of the total cell counts.

H 2 O 2 Toxicity Tests in Batch Cultures
To expose Microcystis to different concentrations of H 2 O 2 , 250 mL samples from the four steady-state chemostats were mixed and diluted with~2000 mL sterile BG-11 mineral medium to reach a total Microcystis biovolume of 100 mm 3 /L in 3 L of mineral medium. This stock culture was used to carry out batch culture experiments in 12 Nalgene plastic bottles (Thermo Fisher Scientific, Pittsburgh, PA, USA) of 250 mL each and without lids. Each bottle received 200 mL of culture and was aerated with compressed air. The temperature was maintained at 25 • C using stainless steel cooling fingers connected to a water bath. H 2 O 2 concentrations added to the batch cultures of Microcystis were 0, 1, 2, 4, 6 and 10 mg/L. Each H 2 O 2 treatment was performed in duplicate. In addition, 10 mg/L of H 2 O 2 was added to two plastic bottles with sterilized BG-11 medium and two plastic bottles with Milli-Q water (Merck KGaA, Darmstadt, Germany), but without Microcystis, to serve as controls for the degradation rate of H 2 O 2 without cells.
We repeated this experiment five times, each time at a different light intensity. The time interval between experiments at different light intensities was about two weeks, to enable recovery of the chemostats to steady state after sampling. Light was provided by white fluorescent tubes (Philips Master TL-D 90 De Luxe 18 W/965, Philips Lighting, Eindhoven, The Netherlands) with incident light intensities of 0, 15, 50, 100 and 150 µmol photons·m −2 ·s −1 set by neutral density filters (LEE Filters, Andover, UK) and the distance between the fluorescent tubes and batch cultures. This range of light intensities is commonly found in the first 1-2 m of turbid lakes dominated by cyanobacteria [63,64]. The light spectrum of the white fluorescent tubes covers the entire range of photosynthetically active radiation (PAR, 400-700 nm), but differs somewhat from sunlight, as it has several distinct peaks at, e.g., 435, 489, 545, 589 and 612 nm.
Samples (5 mL) were taken just before adding H 2 O 2 , immediately after adding H 2 O 2 , and every half hour after adding H 2 O 2 for a total of 4 h for the analysis of the photosynthetic vitality and H 2 O 2 concentration. Furthermore, 5 mL samples were taken just before adding H 2 O 2 and 24 h after adding H 2 O 2 for analysis of MC concentrations and cell numbers. Cell numbers and cell volumes were determined using a Casy 1 TTC cell counter with a 60 µm capillary (Schärfe System GmbH, Reutlingen, Germany). Cell size was calculated from cell volume assuming a spherical shape of the cells.

Photosynthetic Vitality
The 5 mL samples were filtered using 1.2 µm pore size 25 mm GF/C filters (Whatman GmbH, Dassel, Germany) placed on a Millipore 1225 Sampling Manifold (Merck KGaA, Darmstadt Germany). The cells on the filters were dark-adapted for 5 min by covering the filters with rubber stoppers on the Millipore filtration unit. The potential photosynthetic yield of the cells was determined using a Mini-PAM fluorometer (Walz, Effeltrich, Germany). The instrument was equipped with an 80 cm long glass fiber cable, mounted in a rubber stopper of the Millipore filtration unit.
After dark adaptation, the rubber stoppers were exchanged with the rubber stopper with the glass fiber cable and the fluorescence measurements were started. The photosynthetic yield (φPSII, also known as the quantum yield of PSII electron transport) was calculated according to Genty et al. [109]: where F 0 and F m are the minimum and maximum fluorescence, respectively. The photosynthetic vitality is defined as the photosynthetic yield of cells treated with H 2 O 2 as a percentage of the photosynthetic yield of cells from control samples not exposed to H 2 O 2 .

Dose-Response Models
The photosynthetic vitality (PV) was described as function of the added H 2 O 2 concentrations, according to the following logistic dose-response model: where A is the estimated EC 50 value (i.e., the H 2 O 2 concentration at which PV is 50%), B is the slope of the dose-response model and C is the photosynthetic vitality of the control samples not exposed to H 2 O 2 (i.e., C was set to 100%). The parameters A and B of the dose-response model were estimated by fitting the model to the photosynthetic vitality measured after 4 h using nonlinear regression, which minimizes the residual sum of squares using an iterative Levenberg-Marquardt algorithm. First, a dose-response model was fitted for each light intensity separately. Subsequently, we applied a pairwise comparison to test whether the PV data of two adjacent light intensities is best described by these two separate dose-response models, or by a single dose-response model that captures the PV data of both light intensities. Model selection was based on the Akaike Information Criterion (AIC). More specifically, we used a modified version of the AIC known as the AICc, which corrects for small sample size according to: where k is the number of parameters, n the number of data points and RSS the residual sum of squares of the model. Accordingly, k = 4 if the PV data of two adjacent light intensities can best be described by two separate dose-response models, whereas k = 2 if a single dose-response model can be used. The approach with the smaller AICc value better describes the data.

Hydrogen Peroxide Analysis
Filtrates obtained from the filtered samples (see Section 4.3) were used for analysis of the H 2 O 2 concentration. A small amount of filtrate (100 µL) was mixed with 100 µL of 2 mmol/L p-nitrophenylboronic acid reagent (Merck KGaA, Darmstadt, Germany) in a 96-well microtiter plate according to Lu et al. [110]. H 2 O 2 -dependent formation of p-nitrophenolate at room temperature was complete in 30 to 45 min, and the color remained stable for several hours. Absorption was measured at the absorption peak of p-nitrophenolate (405 nm) using a SPECTROstar Nano plate reader (BMG Labtech GmbH, Ortenberg, Germany). A calibration curve for H 2 O 2 concentrations ranging from 0.01-10 mg/L was generated using a 33% (w/w) stock solution of H 2 O 2 (VWR, Amsterdam, The Netherlands). For each filtrate, the H 2 O 2 concentration was measured in quadruplicate. Pilot experiments showed that losses of H 2 O 2 by the filtration procedure were <1%.

Microcystin Analysis and Degradation
To determine cellular MC contents and the MC concentrations in the water phase, the cells on the filters (defined as intracellular MC) and in the filtrates (extracellular MC) were stored at −20 • C and subsequently freeze-dried. The freeze-dried samples were resuspended, and MC was extracted in 1 mL of 75% MeOH according to Tonk et al. [111] and analyzed using high performance liquid chromatography (HPLC) according to Van de Waal et al. [112], using a Shimadzu LC-20AD HPLC system with a SPD-M20A photodiode array detector (Shimadzu, Kyoto, Japan). Microcystis PCC 7806 produces two MC variants, [Asp3]MC-LR and MC-LR [113], but peaks of the two MC variants could not be completely separated. Therefore, both peaks were summed and are referred to as MC in the following sections.
To investigate potential degradation of pure MC-LR by H 2 O 2 , this pure MC-LR (ENZO Life Science BVBA, Brussels, Belgium) was dissolved in 100% MeOH and diluted with BG-11 medium to a final concentration of~230 µg/L of MC-LR in a total volume of 1.5 L. Subsequently, 100 mL samples were taken and exposed to different H 2 O 2 concentrations at different light intensities using the same set-up as for the batch culture experiments described in Section 4.2.

Effect of Light Color
Microcystis samples were diluted to OD 750 = 0.3 with freshly made, sterile BG-11 medium, and subsequently 3 mL aliquots were transferred to 12-well plates. We used a total of eight plates that were exposed to two different light intensities (5 or 50 µmol photons·m −2 ·s −1 ) of either blue light (450 nm), green light (530 nm), orange light (630 nm) or far red light (730 nm) provided by custom-made light emitting diode (LED) light panels (Technology Centre, University of Amsterdam, The Netherlands in collaboration with Philips Lightning B.V., Eindhoven, The Netherlands). The light spectra of the LEDs had a full width at a half maximum of~20 nm, as confirmed with a RAMSES-ACC-VIS spectroradiometer (TriOS, Oldenburg, Germany). Light intensities were measured with a LI-COR LI-250 quantum photometer (LI-COR Biosciences, Lincoln, NE, USA).
On each 12-well plate, four wells served as control (0 mg/L H 2 O 2 ), four other wells received 2 mg/L H 2 O 2 and the four remaining wells received 5 mg/L H 2 O 2 . The photosynthetic vitality of the Microcystis cultures in the well-plates was monitored through the bottom glass of the wells for the first 4 h after H 2 O 2 addition with a PAM fluorometer, as described in Section 4.3.

Supplementary Materials:
The following are available online at http://www.mdpi.com/2072-6651/12/1/18/s1, Figure S1: Effects of light intensity on minimum fluorescence (F 0 ) and maximum fluorescence (F m ) after addition of different H 2 O 2 concentrations, Figure S2: Pure microcystin-LR (MC-LR) concentrations 24 h after addition of different H 2 O 2 concentrations at three different light intensities, Table S1: Photosynthetic yield (Fv/Fm) during the first 4 h after addition of different H 2 O 2 concentrations and exposure to different light intensities, Table S2: Parameter estimates of the dose-response models.