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Embryotoxic effects of tissue-specific antiserum in the chick embryo.

Antiserum against adult chicken brain was obtained in rabbits. The antiserum was absorbed on chicken kidney and serum. Subsequently the gamma-globulins were precipitated with half-saturated ammonium sulfate and resuspended in distilled water. At least three neural-specific antibodies were present in the solution. Chick embryos of 33–36 h of incubation were exposed in ovo to this solution and to the same preparation of normal rabbit serum. Control embryos were normal. The neural-specific antibodies had specific cytotoxic effects on the neural epithelium of embryos. All other tissues were normal and healthy.