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Optimization of propagation conditions for mycorrhiza helper bacteria Bacillus pumilus HR10 strain

Previous research indicated that Bacillus pumilus HR10 could significantly promote the growth of Pinus massoniana in association with ectomycorrhizal fungi. With the purpose of expanding the application of this strain,the fermentation conditions of B. pumilus HR10(p H,fermentation broth,temperature and inoculation amount) were optimized based on our previous culture medium optimization. Our results suggested that the optimal fermentation conditions for the propagation of B. pumilus HR10 were p H 6. 5,fermentation broth 50%,temperature 28 ℃,inoculation amount 5%. The biomass in the fermentation broth showed an increase of 10. 4% comparing with original fermentation conditions.