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Therapeutic Targeting of MYC in Head and Neck Squamous Cell Carcinoma

ABSTRACT MYC plays critical roles in tumorigenesis and is considered an attractive cancer therapeutic target. Small molecules that directly target MYC and are well tolerated in vivo represent invaluable anti-cancer therapeutic agents. Here, we aimed to investigate the therapeutic effect of MYC inhibitors in head and neck squamous cell carcinoma (HNSCC). The results showed that pharmacological and genetic inhibition of MYC inhibited HNSCC proliferation and migration. MYC inhibitor 975 (MYCi975), inhibited HNSCC growth in both cell line-derived xenograft and syngeneic murine models. MYC inhibition also induced tumor cell-intrinsic immune responses, and promoted CD8+ T cell infiltration. Mechanistically, MYC inhibition increased CD8+ T cell-recruiting chemokines by inducing the DNA damage related cGAS-STING pathway. High expression of MYC combined with a low level of infiltrated CD8+ T cell in HNSCC correlated with poor prognosis. These results suggested the potential of small-molecule MYC inhibitors as anti-cancer therapeutic agents in HNSCC.


Introduction
Head and neck squamous cell carcinoma (HNSCC), arising from the oral cavity, oropharynx, larynx, and hypopharynx, is a highly aggressive malignant tumor with poor prognosis. 1 Multidisciplinary management, including surgery, radiation therapy, chemotherapy or combinations of these therapeutic modalities, represent the classical options for managing HNSCC. 2,3 Despite advances in multidisciplinary management, toxicities such as xerostomia, oral mucositis, trismus, radiation caries, and osteoradionecrosis are reported, 2,4 and the 5-year survival rate for patients with HNSCC has not increased over past decades. [5][6][7] Thus, it is essential to explore more effective therapeutic strategies to improve HNSCC prognosis.
The MYC gene, located on human chromosome 8, is one of the most commonly activated genes during tumor development. [8][9][10] MYC overexpression induces tumorigenesis and its proteins are functionally involved in up to 70% of all human cancers. [11][12][13] Several oncogenic signaling pathways, such as WNT, RAS, and PI3K/AKT, might mediate their protumorigenic functions through MYC. 14,15 Given the essential roles of MYC underlying transcriptional activation and tumorigenesis, selective inhibition of MYC has become an attractive anticancer therapy strategy. [16][17][18][19][20] Recently, programmed cell death 1 (PD1) blockade-based immune checkpoint inhibition (ICI) therapy combined with chemotherapy has been approved as a first-line of treatment for recurrent or metastatic HNSCC. Unfortunately, the objective response rates are not very high, and the median response duration is relatively short, suggesting that HNSCC is resistant to ICI therapy. 21 For recurrent and/or metastatic HNSCC, tumor programmed cell death-ligand 1 (PD-L1) expression should be evaluated as an approved ICI therapy within the framework of quality assurance. 22 Very recently, a study showed that MYC amplification regulates PD-L1 expression and is involved in a decreased response to ICI therapy in HNSCC, 23 indicating that MYC might help to predict the efficacy of ICI therapy and to select suitable therapeutic targets.
Various types of inhibitors, including those that target MYC-Max dimerization, MYC transcription/translation suppression, and the destabilization of MYC, have been developed. 24 However, it would be quite challenging to target MYC directly because of its undruggable protein structure. 24 MYCi975, a small molecule inhibitor of MYC, was confirmed to promote MYC phosphorylation and degradation. 25,26 MYCi975 showed favorable pharmacokinetics, in vivo tolerability and efficacy in mouse tumor models. Moreover, treatment with MYCi975 modulated the tumor immune microenvironment by inducing immune cell infiltration. 25 However, the therapeutic effect of MYCi975 on HNSCC remains unknown, and the underlying mechanism still needs to be investigated. In this study, we tested whether silencing MYC expression in HNSCC leads to tumor regression associated with remodeling of the tumor microenvironment. December 2016 were enrolled in this study. This study was approved by the Ethics Committee of Peking University School and Hospital of Stomatology (Approval number PKUSSIRB-2012010).

Cell culture
Human HNSCC cell lines, HN6 and CAL27, were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). These cells were grown in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and antibiotics (streptomycin and penicillin) at 37°C in a 5% CO 2 atmosphere.
A murine head and neck squamous cell line (MSCC1), isolated from 4-nitroquinoline N-oxide (4NQO)-induced murine head and neck squamous cell carcinoma, was generated in our laboratory as previously described. 27 Primary 4NQOinduced mouse HNSCCs were generated as described previously. 28 4NQO (Santa Cruz Biotechnology, Santa Cruz, CA, USA, Cat# 256815) was diluted in the mice's drinking water to a final concentration of 50 μg/mL and was changed weekly. After 16 weeks, all animal cages were reverted to regular water until week 22. Animals were euthanized on week 22 for tissue retrieval. Single-cell suspensions of individual primary oral cavity tumors were made using Collagenase IA (Sigma-Aldrich, St. Louis, MO, USA; Cat#C0130) and cultured in Defined Keratinocyte-SFM medium supplemented with recombinant mouse EGF protein (5 ng/ml) and 1% antibiotic/antimycotic solution. Sequential differential trypsinization was then used to clear fibroblast contamination.

Animals
BALB/c-nude female mice and C57BL/6 J male mice (6-8 weeks old) were housed in a specific pathogen free environment. All animal studies were performed in compliance with the regulations and the Peking University institutional animal care guidelines.
HN6 cells (5 × 10 6 ) and Matrigel were injected into the dorsal subcutaneous region of BALB/c-nude mice. Three weeks after injection, the mice were divided into two groups, and given vehicle or MYCi975 (MedMol, Shanghai, China; Cat#S89011; 100 mg/kg) every 2 days for 4 weeks. MSCC1 cells (5 × 10 6 ) were injected into the dorsal subcutaneous region of C57BL/6 J mice. Four weeks after injection, the mice were divided into four groups and treated using control vehicle, MYCi975, anti-C-X-C motif chemokine receptor 3 (CXCR3), and MYCi975 plus anti-CXCR3 for 4 weeks. Mice were sacrificed and the tumor samples were dissected and isolated immediately. The tumor volume was determined using the volume formula for an ellipsoid: 1/2 × D × d, 2 where D is the longer diameter and d is the shorter diameter.

siRNA transfection and MYCi975 treatment
Cells were grown to 50-60% confluence, and then transfected with small interfering RNA (siRNA) duplexes, siNC (negative control) or siMYC (Aibosi Inc., Shanghai, China) using the JetPrime reagent (Polyplus Transfection, Illkirch, France; Cat#101000046). The siRNA (20 μM) and JetPrime regent (1:1) were dissolved in transfection buffer at a ratio of 1:50. After 10 minutes of incubation at room temperature, the complex was added to DMEM at a ratio of 1:10. The final concentration of the siRNA was 50 nM. For subsequent assays, siRNA transfection was performed 24-48 h before each assay, accompanied by western blot to evaluate the transfection effect.
For drug administration, MYCi975 was added to the culture medium when the cells reached 70-80% confluence.

Transwell assay
After treatment with the siRNA or MYCi975, the cells were resuspended in serum-free DMEM at a concentration of 10 5 cells per well. They were then transferred into the upper compartment of a Transwell chamber (8-μm pore size, Corning Inc., Corning, NY, USA; Cat#3422) in a 24-well plate containing DMEM with 20% FBS. HN6 cells were harvested after 12 h of incubation, while CAL27 cells were harvested after 24 h. The chambers were then stained with 0.1% crystal violet (Solarbio, Beijing, China; Cat#G1064) for 30 min. Images were obtained under an optical microscope and analyzed using ImageJ (NIH, Bethesda, MD, USA).

Wound healing assay
After treatment with the siRNA or MYCi975, the confluent layer of cells was scratched using a 1 mL pipette tip, and incubation in DMEM was continued. Images were taken at 18 h for HN6 cells and 36 h for CAL27 cells under an optimal microscope. Wound healing was quantified by measuring the width of the wound healing. Each experiment was independently performed at least three times.

Quantitative real-time reverse transcription PCR (qRT-PCR)
Total RNA was prepared using the TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA; Cat#15596026), and 500 ng of RNA was reverse transcribed to cDNA using a reverse transcription kit (Takara, Dalian, China; Cat#RR036A). The cDNA was then quantified using a SYBRGreen kit (Roche, Basel, Switzerland; Cat#04913914001). GAPDH was used as an internal control. The qRT-PCR primers used in this study are listed in Table S1.

Comet assays
Single cell gel electrophoresis comet assays were performed using an SCGE assay Kit (Enzo Life Sciences, Farmingdale, NY, USA; Cat# ADI-900-166). Following treatment, the cells were mixed with low melting point agarose at a volume ratio of 1:50, and 75 μL aliquots were loaded onto pre-warmed slides. The slides were incubated in pre-chilled lysis solution for 1 h and then in pre-chilled alkaline solution for 30 min. Electrophoresis was run at 25 V in TBE buffer for 20 min. Comets were stained with CYGREEN dye for 30 min and imaged. At least 100 individual cells per sample were evaluated in duplicate using the CASP Version 1.2.2 analysis tool (СASPlab, Wroclaw, Poland).

Statistical analysis
SPSS 21.0 (IBM Corp., Armonk, NY, USA) was used for the statistical analysis. All in vitro experiments were repeated at least three times, and the in vivo experiments were repeated at least once. Student's t test was used to analyze the data between two groups. The differences among multiple groups were evaluated using one-way analysis of variance (ANOVA). A χ 2 test was used to analyze the relationship between MYC expression, CD8 + T cell infiltration density, and clinicopathological factors. Survival curves were constructed using the Kaplan-Meier method and the curves were compared using a log-rank test. The multivariate Cox regression model was applied to simultaneously adjust all potential prognostic variables (P < .1 by a log-rank test in univariate analysis). Correlations were analyzed using the Pearson test. P < .05 was considered statistically significant.

MYC is overexpressed in HNSCC and correlates with poor prognosis
MYC expression was tested in 20 pairs of HNSCC and adjacent non-tumor tissues. The different expression patterns of MYC were confirmed using immunostaining (Figure 1a). The results showed that MYC was increased in HNSCC samples compared with their nonmalignant counterparts (Figure 1b). We then tested MYC in 121 HNSCC samples using immunochemical assays. The results showed that the MYC level correlated with the tumor size, lymph node metastasis, and clinical stage (Table S2). Then, according to the average level of MYC, patients were divided into two groups: high MYC expression and low MYC expression. We confirmed that patients with high MYC levels had shorter survival than those with low MYC levels ( Figure 1c). To explore the therapeutic effect of MYCi975 on HNSCC in vivo, we constructed a subcutaneous dorsal cell line-derived xenograft model in nude mice. The tumor volume and weight of the xenografts treated with MYCi975 were reduced comparted with those treated using control vehicle (Figure 3f-h). Compared with that in the control group, the weight of the   mice treated with MYCi975 did not change significantly, suggesting that the mice tolerated MYCi975 treatment (Figure 3i).

MYC inhibition increases the tumor cell-intrinsic immune response through the cGAS-STING signaling pathway
Previous studies showed that MYC is associated with the DNA damage response. [29][30][31] Consistently, we confirmed that the level of p-H2A.X, a specific marker for DNA damage, was significantly increased in cells treated with MYCi975 ( Figure 4 a and b). Then, we used a comet assay, a gel electrophoresis-based method that can be used to measure DNA damage in individual eukaryotic cells. Under an electrophoretic field, damaged cellular DNA (containing fragments and strand breaks) is separated from intact DNA, yielding a classic "comet tail" shape under the microscope. The extent of DNA damage is usually estimated visually by comet tail measurement. Comet assays showed that MYCi975 treatment significantly increased the comet tail moment in HN6 and CAL27 cells (Figure 4 c and d), indicating increased DNA damage. To examine whether DNA damage in the nucleus induced by MYC inhibition could result in the accumulation of cytosolic dsDNA, we stained live HNSCC cells with PicoGreen, a dsDNA-specific vital dye. It was reported that PicoGreen also stains mitochondrial DNA; 32 therefore, mitochondrial DNA was visualized using MitoTracker staining to exclude its interference during the quantification of cytosolic dsDNA. Multiple PicoGreen staining areas were observed in the cytoplasm of HN6 and CAL27 cells, which did not overlap with the MitoTracker staining, upon MYCi975 treatment, indicating that MYCi975 induced the accumulation of cytosolic dsDNA (Figures 4e).
DNA damage can activate a tumor immune response. 28,[33][34][35] The accumulation of cytosolic DNA can activate the cyclic guanosine monophosphate (GMP)-AMP synthase-stimulator of interferon (IFN) gene (cGAS-STING) signaling axis by the sequential phosphorylation of STING, TBK1, and IRF3. Then, phosphorylated IRF3 dimerizes and translocates to the nucleus to trigger the expression of IFN and IFN-regulated chemokines, 36 including C-X-C motif chemokine ligand (CXCL)9, CXCL10, and CXCL11. 37 Western blot analysis showed that MYC inhibition enhanced the levels of phosphorylated STING, TBK1, and IRF3 in HN6 and CAL27 cells (Figure 4f). Consequently, MYC inhibition increased the mRNA expression of IFNβ, CXCL9, CXCL10, and CXCL11 in HN6 and CAL27 cells (Figures 5a-d). Immunofluorescent staining also demonstrated that phospho-IRF3 (p-IRF3) was increased in HN6 and CAL27 cells treated with MYCi975 (Figure 4g), which further confirmed that MYC exerts its inhibitory effects by activating the cGAS-STING signaling pathway.

MYC inhibitor promotes CD8 + T cell infiltration in vivo
CXCR3 is activated by CXCL9, CXCL10, and CXCL11, and then mediates the recruitment of CD8 + T cells into tumors. 38 MYC knockdown induced the expression of CXCL9, CXCL10, and CXCL11; therefore, we proposed that MYCi975 could promote CD8 + T cell infiltration in HNSCC. MSCC1 cells were injected into the subcutaneous dorsal region of immunecompetent mice to construct a syngeneic murine model. After MYCi975 treatment, MYC protein expression was reduced, while CD8 + T cell infiltration increased ( Figure 6 a and b). To further confirm that the MYC inhibitor promoted CD8 + T cell infiltration through CXCR3 mediation, mice were divided into four groups and treated with vehicle, MYCi975, anti-CXCR3, and MYCi975 plus anti-CXCR3 (Figure 6c). The volume and weight of the tumor xenografts showed that anti-CXCR3 partially reversed the MYCi975-mediated inhibition of HNSCC development (Figure 6d-f) and the increase in CD8 + T cells ( Figure 6 g and h).

High MYC combined with low CD8 + T cell infiltration in HNSCC correlates with poor prognosis
The numbers of infiltrated CD8 + T cells were increased in HNSCC treated with MYCi975; therefore, we investigated the relationship between MYC expression and CD8 + T cells in HNSCC samples. The results showed that MYC expression correlated negatively with CD8 + T cell numbers in HNSCC (Figure 7 a and b). Moreover, the number of infiltrated CD8 + T cells in HNSCC correlated with the pathological stage, lymph node metastasis, clinical stage (Table S3), and 5-year overall survival (Figure 7c). Given that MYC expression and CD8 + T cell infiltration correlated negatively in clinical HNSCC samples, we further analyzed patient subgroups based on the combination of MYC expression and CD8 + T cell infiltration. With the average MYC expression and CD8 + T cell infiltration chosen as the cutoff points, two subgroups were assigned: the "high MYC and low CD8 + T cell" group and the "low MYC or high CD8 + T cell" group. Kaplan-Meier curves showed that patients with high MYC expression and low CD8 + T cell infiltration correlated closely with poor overall survival (Figure 7d). To explore the independent factors influencing the prognosis of HNSCC, we conducted a univariate analysis of the impact of clinical characteristics of HNSCC on 5-year overall survival ( Figure S1). Factors with P-values < 0.1 were then included in a Multivariate Cox regression model to identify independent factors associated with overall survival. The multivariate Cox regression model showed that high MYC expression and low CD8 + T cell infiltration in HNSCC, and clinical stage (III/Ⅳ) were associated with poor prognosis in terms of 5-year overall survival, independent of other factors (Table 1).

Discussion
Growing evidence indicates that MYC regulates tumorigenesis through a variety of mechanisms, and is functionally involved in up to 70% of all human cancers. 9 Multiple studies demonstrated that MYC plays an important role in tumorigenesis, and is regarded as an attractive target for cancer therapy. [16][17][18][19][20]39 In this study, we confirmed in a relatively large patient population that MYC was increased in HNSCC, and correlated with lymph node metastasis and poor prognosis. Moreover, we focused on testing whether MYCi975, a novel MYC small molecule inhibitor, could inhibit HNSCC growth in preclinical animal models. Mechanistically, while MYCi975 induced Immunofluorescent staining and quantification of p-H2A.X (red) in HN6 and CAL27 cells with or without MYCi975 treatment. Nuclei were stained using DAPI (blue). Means ± SD are shown from three independent experiments. Scale bar, 50 μm. **P < .01 by an unpaired Student's t test. (c-d) Representative images and quantification of DNA Comet assays in HN6 and CAL27 cells treated with MYCi975. More than 100 cells were analyzed per group. Means ± SD are shown. Scale bar, 100 μm. **P < .01 by an unpaired Student's t test. (e) Confocal microscopy images showing cytosolic DNA accumulation and quantification in HN6 and CAL27 cells with MYCi975 treatment. Double-stranded DNA (dsDNA) was stained using PicoGreen (green). Mitochondria and nuclei were stained using MitoTracker (Red) and DAPI (blue), respectively. The white arrows indicate cytosolic dsDNA. Scale bar, 10 μm. More than 100 cells were analyzed per group. Means ± SD are shown. **P < .01 by an unpaired Student's t test. (f) Western blot analysis of phosphorylation of STING, TBK1, and IRF3 in HN6 and CAL27 cells treated with MYCi975. GAPDH was used as the internal control. (g) Immunofluorescent staining of p-IRF3 (green) in HN6 and CAL27 cells after MYCi975 treatment and their quantification. Nuclei are stained using DAPI (blue). Means ± SD are shown from three independent experiments. Scale bar, 50 μm. **P < .01 by an unpaired Student's t test.
HNSCC cell apoptosis, it also activated antitumor immunity by recruiting CD8 + T cells (Figure 7e). Our preclinical studies suggest that targeting MYC using MYCi975 might be a new strategy to effectively inhibit HNSCC growth.
Previous studies investigated the role of targeting MYC for tumor therapy by inhibiting MYC indirectly, such as using bromodomain containing 4 (BRD4) inhibitors. 17,40,41 JQ1 is a well-known BRD4 inhibitor that can inhibit MYC in various solid tumors. [42][43][44] However, its inhibition of MYC in HNSCC is controversial. Previous studies have shown that JQ1 can significantly reduce the expression of MYC and inhibit HNSCC growth. 40,45,46 By contrast, a recent study showed JQ1 only mildly inhibited MYC in HNSCC, and uniquely controlled a set of cancer stemness-associated genes instead of MYC. 41 Thus, the inhibition of MYC by JQ1 in HNSCC requires further study.
MYCi975 increases the number of infiltrated T cells, and sensitizes prostate and lung cancers to anti-PD1 immunotherapy. 25 Similarity, our results confirmed that MYCi975 activated the anti-tumor immune response and increased the number of tumor-infiltrating CD8 + T cells in HNSCC. Cytotoxic CD8 + T lymphocytes have an important antitumoral activity by recognizing and destroying tumor cells. The presence of CD8 + T lymphocyte infiltration is therefore related to a favorable prognosis. 47 Growing evidence suggests that tumor resistance to ICI therapy is probably caused by lack of CD8 + T cell infiltration into the tumor microenvironment after treatment. 48 HNSCC induces an immunosuppressive tumor microenvironment with low numbers of tumorinfiltrating lymphocytes, 27,49,50 suggesting that HNSCC might have an intrinsic anti-immunotherapy mechanism. MYC exerts multiple biological effects on cellular programs that influence both cell-intrinsic biology as well as the tumor immune microenvironment. 10 MYC contributes to cancer cell immune evasion, and prevents tumor cells from being attacked by immune cells. 10 We analyzed 121 human HNSCC Previous studies showed that MYC inactivation leads to cellular senescence and DNA damage. 51,52 We demonstrated that MYCi975 activates the tumor cell-intrinsic immune response through the cGAS-STING signaling pathway. Initially, we observed that the level of p-H2A.X, a well-known marker of DNA damage, was increased in HNSCC after MYCi975 treatment. Then, consistent with DNA damage, we confirmed the accumulation of cytosolic dsDNA, which subsequently activated the STING-TBK1-IRF3 pathway to induce the expression of the type I IFN chemokines (CXCL9, CXCL10, and CXCL11). CXCR3 is a cytotoxic T cell-associated chemokine receptor that is activated by its chemokine ligands CXCL9, CXCL10, and CXCL11. 38,53 The results of in vivo rescue assays showed that anti-CXCR3 partially reversed MYCi975-mediated antitumor effects and increased the number of CD8 + T cells, indicating that MYCi975 regulated the tumor immune microenvironment through chemokines.
ICI therapy has emerged as a new type of clinical treatment for cancer. However, the response rate of HNSCC to ICI therapy is only around 20-30%, 21 and the majority of HNSCC tumors are non-responsive. Thus, combined therapy comprising ICI and other agents is required. Combining anti- Figure 7. CD8 + T cell infiltration density is negatively associated with the MYC protein levels in patients with HNSCC. (a) Immunostaining of human HNSCC samples showing that high MYC levels correlated with low CD8 + T cell infiltration density and low MYC levels correlated with high CD8 + T cell infiltration density in the same view of the slice. Scale bar, 50 μm. (b) CD8 + T cell infiltration density correlates negatively with MYC protein level in human HNSCC samples (n = 121). The Pearson correlation coefficient of linear regression was used to determine the correlation between different proteins. (c) Kaplan-Meier curves with log-rank tests showing that patients with high CD8 + T cell infiltration density had longer survival (P = .002). (d) Kaplan-Meier curves with log-rank tests showing that patients with "high expression of MYC and low CD8 + T cell infiltration" had significantly shorter survival than those with "low expression of MYC or high CD8 + T cell infiltration" (P < .001). (e) A diagram of the mechanism by which MYC inhibition eliminates tumors and activates antitumor immunity.
PD-1/PD-L1 therapy with other treatment methods such as surgery, chemotherapy, radiotherapy, molecular targeted therapy, or other immunotherapies has been approved to treat recurrent or metastatic HNSCC. 3 Our previous study showed that targeting BMI1 in HNSCC could enable ICI therapy to inhibit metastatic tumor growth and prevent tumor relapse. 28 Growing evidence shows that response to checkpoint inhibition is limited to immunogenic tumors that express checkpoint proteins, such as PD-L1 and/or contain tumor infiltrating CD8 + T cells within the local tumor microenvironment. 54 Previous studies showed that MYC is a regulator of PD-L1, 23,43,55 and the combination of JQ1 and ICI therapy resulted in synergistic inhibition of pancreatic cancer growth. 43 Very recently, MYC expression has been found to correlate with non-responsiveness to ICI therapy in HNSCC. 56 Our findings have important implications in developing new treatments for HNSCC using MYCi975 to activate tumor cell-intrinsic immune responses. A large number of PD1 blockade-based combination therapies are in clinical trials, suggesting that MYCi975 could be used to enhance the sensitivity of HNSCC to ICI therapy in the future.
Given that MYC inhibition increased the number of tumorinfiltrated CD8 + T cells, we combined MYC expression and CD8 + T cell infiltration to predict the prognosis of patients with HNSCC. The results showed that patients with "high MYC expression and low CD8 + T cell infiltration" had the worst 5-year overall survival. These results demonstrated that MYC expression combined with CD8 + T cell infiltration correlates closely with HNSCC prognosis. Recently, depth of invasion (DOI) and extranodal extension (ENE) in oral cavity cancer have been considered as important factors in determining prognosis. 57,58 In the newly published 8th edition of the TNM, 59 DOI and ENE were included. Thus, we will further explore the relationship among DOI, ENE and HNSCC prognosis in future research.
There are still some limitations to this study. The "cell linederived xenograft model" does not fully reflect the diversity and heterogeneity of tumors. Patient-derived tumor xenograft (PDX) models are based on the implantation of fresh cancer tissue fragments from patients directly into immunodeficient mice, which represents a reliable preclinical model because of the retention of cellular heterogeneity and the molecular characteristics of the original tumor. 60 Thus, our results require further validation using a PDX model.
HNSCC frequently metastasizes to cervical lymph nodes, and the poor prognosis of patients with HNSCC is closely associated with lymph node metastasis. 61,62 Although a subcutaneous tumor model is the most popular assessment system for the in vivo efficacy evaluation of novel anti-cancer medicine candidates, it is not a suitable animal model to assess tumor metastasis. The effect of MYCi975 on the inhibition of HNSCC lymph node metastasis still needs to be confirmed via an orthotopic HNSCC model, in which we orthotopically transplant HNSCC cells into the mouse tongue. 63 In summary, our results demonstrated that MYC serves as a tumorigenic factor, driving HNSCC progression. MYCi975 could inhibit tumor growth and regulate tumor CD8 + T cell infiltration through chemokines by activating the cGAS-STING pathway in HNSCC. This indicated that targeting MYC in HNSCC could be an effective therapeutic strategy. We proposed MYCi975 as a potential therapeutic targeted drug for clinical trials to treat HNSCC.