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A novel application of capnography during controlled human exposure to air pollution

Background The objective was to determine the repeatability and stability of capnography interfaced with human exposure facility. Methods Capnographic wave signals were obtained from five healthy volunteers exposed to particle-free, filtered air during two consecutive 5 min intervals, 10 min apart, within the open and then the sealed and operational human exposure facility (HEF). Using a customized setup comprised of the Oridion Microcap® portable capnograph, DA converter and AD card, the signal was acquired and saved as an ASCII file for subsequent processing. The minute ventilation (VE), respiratory rate (RR) and expiratory tidal volume (VTE) were recorded before and after capnographic recording and then averaged. Each capnographic tracing was analyzed for acceptable waves. From each recorded interval, 8 to 19 acceptable waves were selected and measured. The following wave parameters were obtained: total length and length of phase II and III, slope of phase II and III, area under the curve and area under phase III. In addition, we recorded signal measures including the mean, standard deviation, mode, minimum, maximum – which equals end-tidal CO2 (EtCO2), zero-corrected maximum and true RMS. Results Statistical analysis using a paired t-test for means showed no statistically significant changes of any wave parameters and wave signal measures, corrected for RR and VTE, comparing the measures when the HEF was open vs. sealed and operational. The coefficients of variation of the zero-corrected and uncorrected EtCO2, phase II absolute difference, signal mean, standard deviation and RMS were less than 10% despite a sub-atmospheric barometric pressure, and slightly higher temperature and relative humidity within the HEF when operational. Conclusion We showed that a customized setup for the acquisition and processing of the capnographic wave signal, interfaced with HEF was stable and repeatable. Thus, we expect that analysis of capnographic waves in controlled human air pollution exposure studies is a feasible tool for characterization of cardio-pulmonary effects of such exposures.


Background
The primary objective of current and future air pollution studies is to further understand the mechanisms for epidemiologic data on increased morbidity and mortality due to exposure to a ambient pollutants, e.g. fine particulate air pollution, i.e. particles of aerodynamic diameter < 2.5 μm (PM 2.5 ) [1][2][3][4][5]. In addition, the next objective is to link the data from animal studies [6] with clinical evidence related to adverse cardio-pulmonary and vascular events; in particular, coronary and cerebro-vascular events related with such exposure [7][8][9][10]. To this end, environmental air pollution studies aim to reveal the physiologic changes and molecular mechanisms that follow inhalation of gaseous and particulate pollution and lead to a systemic inflammatory response. Moreover, they aim to provide plausible explanations of the association between air pollution exposure and morbidity/mortality from cardio-pulmonary and vascular etiology. Understanding these outcomes is of paramount importance in susceptible populations such as children, the elderly, asthmatics, patients suffering from chronic obstructive pulmonary disease (COPD), cardiovascular diseases and diabetes.
Different health outcomes and indices have been assessed during controlled human exposures to gaseous and particulate air pollution: ECG and lung function changes [11,12], heart rate variability [13][14][15], blood and sputum markers of inflammation -cellular variability and mediators including interleukin 6 (IL-6), leukotriene B4 (LTB 4 ), tumor necrosis factor alpha (TNFα,) interleukin 8 (IL-8), endothelins and fibrinogen, [13,16]. Acute PM 2.5 exposure can promote systemic inflammation by activating the capillary endothelium in the pulmonary circulation [17,18]. Therefore, we redirected our interests in detecting acute effects of PM 2.5 exposure from lung mechanics to gas exchange surface of the alveolar-capillary membrane. Our fine concentrated ambient particles (CAP) exposure facility can concentrate particles sized 0.15 -2.5 μm in aerodynamic diameter. Due to geometry of the airways, fine particles (PM 2.5 ) can be inhaled deep into the small conducting bronchioles and the alveoli. If adverse effects of PM 2.5 are to be measured, a major site that could be examined is the alveolar-capillary membrane. Therefore, we considered capnography as the most suitable procedure that can provide real-time information about alveolar ventilation, pulmonary perfusion, respiratory pattern, and CO 2 elimination/production. Clinically, capnography has been used in concert with pulse oxymetry to detect adverse respiratory events during anesthesia [19,20]. In addition, capnography has been used in emergency rooms and during emergency transportation [21][22][23][24]. However, although widely used in clinical and prehospital settings, an extensive literature search confirmed that capnography has not been used as a procedure or out-come measure in settings of controlled human air pollution studies.
In our previous studies [17,25], we could not detect any statistically significant changes in standard pulmonary function measures, such as forced expiratory volume in the 1 st second (FEV 1 ) or lung diffusing capacity for carbon monoxide (D L CO). However, we have reported a constriction of the brachial artery immediately following a 2-hour CAP + O 3 exposure [18] and an acute increase in diastolic blood pressure in healthy adults during controlled exposure to CAP ± O 3 [26]. We hypothesize that "real-time" capnography would be a more sensitive or even specific marker of the inflammation induced by pollutants (CAP ± O 3 ) at the alveolar-capillary membrane, as well as pollutant-induced airway constriction, rather than more gross, effort dependent functional tests such as spirometry and lung diffusion. Since events at the alveolar-capillary membrane are an instantaneous reflection of ventilatory/ cardiovascular function, any compromise of the ventilation-perfusion relationship, which may result after breathing CAP ± O 3 , could have some detectable consequences. Therefore, capnography as a non-invasive, instantaneous physiological measurement, which could provide insight into alveolar ventilation, physiological dead space changes, pulmonary perfusion, ventilationperfusion mismatch, changes of cardiac output and respiratory patterns, seems to be a potentially valuable and needed procedure in air pollution research.
In this study, we tested acquisition of the capnographic wave signal for stability and repeatability through customized hardware & software components interfaced with the human exposure facility of The Gage Occupational and Environmental Health Unit, St. Michael's Hospital and University of Toronto. The HEF design and exposure characterization have been described in details elsewhere [17,25]. See Figure 1 showing HEF with the door open.

Hardware and Software Setup
See Figure 2 for the schematic of the hardware interfaced with the HEF, which was used for this procedure. In order to record and analyze instantaneous CO 2 digitized waveforms, we chose the commercially available Oridion Microcap ® CO 2 portable bedside capnograph (Oridion Systems Ltd, Microcap ® , model #CS-04178) [27]. It uses Microstream ® Technology with low-flow, sidestream aspiration vs. traditional high-flow or mainstream monitors [28,29]. This monitor has a low sampling rate of 50 ml/ min and an accuracy of EtCO 2 readings of ± 2 mmHg in the range of 0-38 mmHg and ± 5% of the reading in the range of 39-99 mmHg, when steady state is reached. EtCO 2 accuracy is maintained for up to 80 breaths/min. The system response time, including delay and rise time, is typically 2.45 seconds, with a maximum 2.9 seconds. In addition, proprietary Microbeam IR source generates only the specific wavelengths characteristic of the CO 2 absorption spectrum. This IR source illuminates the microsample cell and reference channel, where the infrared emission exactly matches the absorption spectrum of the CO 2 molecule, thus other gases in the sample such as N 2 O, O 2 and water vapor, do not affect the CO 2 reading. The capnograph has a built-in self-check procedure for verification of validity of the recorded signal. These features make it suitable for our HEF setting, in which we expose non-intubated healthy subjects and subjects with mild pulmonary or cardiovascular disease.
The capnograph we used, had a factory digital-outputonly, although, as an option the manufacturer provided a Digital-to-Analog Converter (CS-07143) [30], which was used to process out 4 digital signals including ground, CO 2 wave, respiratory rate and CO 2 measurement validity (indicates invalidity raising output voltage to full scale). At full scale, 0.9 V equals 100 mmHg for the CO 2 output and 150 breaths/min for the respiratory rate output. A voltage of 1.0 V indicates invalidity due to sampling line disconnection, auto zero, and/or malfunction in all channels. An analog output from DA converter was directed to the terminal board ADAM -3968 SCSI 68P by Super Logics Inc. and further by an expansion cable CS-G56 as a single-ended connection to ADAC MF-5500, 12-bit, 100-kHz PCI data acquisition board [31]. The CO 2 wave signal resolution obtained through entire setup (capnograph, DA converter and AD card) was 0.27874 mmHg over the full scale of 100 mmHg. The acquired signal was captured by WinView software v. 2.9 from Super Logic Inc [32], using the continuous sampling mode with 20 samples per second, board DMA and smooth scanning mode. Logged data were saved as an ASCII file on the hard drive. The The photo of HEF with door open Figure 1 The photo of HEF with door open.
computer used was a Dell Optiplex GX270 with 3.2 GHz Pentium 4 processor and 512 Mb RAM. Saved ASCII files were imported into WinDaq Waveform Browser version 2.39 by DATAQ Instruments Inc. [33], where capnographic waves were analyzed for acceptability according to a Landis score [34]. Variables of interest were measured and entered into an MS Access 2003 database.
The exhaled CO 2 was sampled using two customized Microstream ® EtCO 2 adult NIV Lines™ [35], joined together. We did not want to place the capnograph itself within the sealed HEF, which is at sub-atmospheric pressure (11 mmHg), and slightly higher temperature and relative humidity compared to laboratory and ambient conditions, respectively. The first line (NIV-1) had the capnograph end cut off and that end was attached inside the HEF to the 20G diameter custom needle port, installed through Plexiglas window. The subject used the patient end as a nasal probe with prongs placed comfortably within the nostrils. The second line (NIV-2) had the patient end cut off and that end was attached to the other side of the needle port. The capnograph end of this line was attached to the sampling line port of the capnograph itself. The total length of sampling line, joined in this fashion, was 4.3 m.

Subjects
Two tests (EXP#1 and EXP#2) were carried out on five healthy, non-smokers (4 males and 1 female), aged 35-46. Continuous capnographic wave recordings were obtained twice on each subject, 10 min apart, while seated inside the HEF, first with the door open and then with the door sealed under operational conditions. Subjects had the sampling line nasal prongs placed inside their nostrils, with a particle delivery/CO 2 removal mask over their nose and mouth. Monitoring and routine testing was carried out as in our human exposure studies during a filtered air control exposure [17,25]. One subject had variable and Capnography components added to existing HEF design Figure 2 Capnography components added to existing HEF design. This illustration presents customized hardware & software components for the capnographic wave signal acquisition, interfaced with existing HEF used for the air pollution studies. completely unacceptable waveforms due to periodical sniffling during the tests, thus we analyzed data for four subjects. Immediately before and after each capnographic waveform recording, resting expired tidal volume and respiratory rate were measured using a VMM-401 flow turbine by Interface Associates [37]. It was essential to directly measure tidal volume and respiratory rate, as changes in these parameters can modify the EtCO 2 [38]. The protocol was approved by the Research Ethics Board of St. Michael's Hospital, Toronto.

Data and Statistical Analysis
We measured acceptable capnographic waves for two sets of variables (wave parameters and wave statistics) and two ventilatory variables, shown in Table 1. Selected capnographic wave parameters are described in the literature with defined physiologic meaning and with the ability to characterize certain ventilatory changes [34,39,40]. Capnographic wave signal statistics represented standard statistical parameters, noting that RMS is derived as true RMS by means of an appropriate algorithm within WINDAQ software, and in this case represented mean expired CO 2 (expressed in mmHg) over the expiratory phase of one breath. The mathematical definition of true RMS used by WINDAQ to derive this statistics is where: v n = instantaneous voltage and W = window size [41].
Measurements such as mean blood pressure and/or flow from a pulsatile pressure, airflow waveform and of course capnographic wave are non-sinusoidal waveforms that require a true RMS calculation. Breathing pattern, low respiratory rate and large tidal volume, can influence the shape of the capnographic wave, particularly the slope of phase III of the capnographic wave [42]; therefore, we recorded these parameters for the purpose of the quality control of the recorded capnographic wave parameters and statistics during the two tests. See Figure 3 showing the example of typical capnographic wave analysis, using WinDaq software.
From the list of variables in Table 1

Results
The results of the measurements for monitored variables are presented in Tables 2, 3, and 4. Summary data for EtCO 2 parameters, comparing EXP#1 and EXP#2 for the four subjects are shown in

Discussion
We added customized hardware and software components to interface with the HEF in order to utilize the advantages of capnography over the standard tests of lung function. We chose to use capnography in the HEF as we expect that capnography will be a useful tool for the detection of changes in ventilation during human exposure studies. Capnography, as a non-invasive, real-time physiologic measurement of adequacy of ventilation and perfusion, has found its role in a variety of medical disciplines including anesthesiology, critical care, emergency medicine, cardiology, pediatrics, neurology and respiratory care. Current capnographic hardware and software is designed for these aforementioned environments. As such, we did not find a commercially available, ready-to-go solution for the implementation of capnography in the experimental environment of human air pollution exposure studies. Our goal was to use relatively inexpensive and commercially available hardware com-ponents to acquire EtCO 2 wave signals within sealed and operational HEF, but with acceptable signal quality. Our first choice for the capnograph was the Microcap ® CO 2 portable bedside monitor, with nominal performance features adequate for our study's setup. However, due to the absence of an analog output, this monitor needed an additional digital-to-analog converter. We used one supplied as an option by the original manufacturer, designed to be used with this particular model of capnograph. Further processing of the four analog signals (EtCO 2 wave, RR, EtCO 2 wave signal validity and ground) utilized the standard solution (terminal board, expansion cable and MF 5500 AD card). Although the signal was less than 1.0 V, due to the low level of noise, we found that a single ended connection was adequate. We performed zero CO 2 calibration of the capnograph with 100% O 2 , in order to measure the system signal noise for the overall hardware and software setup. The average noise level was 0.0052 ± 0.016 V and RMS was 0.0168 V. Signal-to-noise ratio (SNR) over the full scale of 100 mmHg was 34.59 dB. We found the WinView data acquisition software easy to install and setup using the MF 5500 AD board. The EtCO 2 An additional concern, related to the EtCO 2 sampling line required a customized solution. We used two original sampling lines to make one custom line, which attaches on both sides of the custom-made needle port screwed into the plexiglas HEF window. Accordingly, we did not observe additional delay added to frequency response of the monitor. We considered the possibility that sampling through a longer line than originally designed and recommended by the manufacturer could also affect the performance of the capnography monitor. However, we did not observe that sampling in this way affected the reading of EtCO2 and RR on the capnograph itself. Mason et al. [36] showed that an experimental sampling line as long as The example of capnographic wave analysis using WinDaq software Figure 3 The example of capnographic wave analysis using WinDaq software. Screenshot of WinDaq windows showing the measurement of slope3 of the typical capnographic wave.
9.0 m was equally reliable as the standard 3.0 m long one, which agrees with our observation.
Comparisons of the differences between capnographic measurements obtained under two different conditions, relevant to our design of human exposure studies (open and sealed HEF), did not yield any statistical significance. Yet, some differences existed in absolute values, but allocation of these differences among observed measures was to be expected. In the group of EtCO 2 wave parameters, the variable with the lowest CV was the zero-corrected EtCO 2 , 3.89% and 3.62%, followed closely by EtCO 2 , 4.14% and 4.48%. Verschuren et al. [43] considered CV of the EtCO 2 less than 5% as an indicator of steady-state status for their patients The EtCO 2 is the main output value of the capnograph and correction of this value with the minimum-recorded value, for a particular wave, increased the accuracy of true EtCO 2 reading. Among seven out of eight tests, we found that the range of recorded zero values for EtCO 2 was between 0 and 0.55664 mmHg (1.42% of maximum-recorded EtCO 2 of 39.167 mmHg). Surprisingly, we found that one subject's exposure (EXP#2 for that subject, with 15 acceptable analyzed waves) had recorded a zero range between 1.4274 to 2.8547 mmHg (7.29% of the maximum-recorded EtCO 2 of 39.167 mmHg). Reviewing the lab log for this test, we found that 5 min after the enclosure was sealed, the recorded CO 2 within the HEF was approximately 0.38%. This could have happened, because the particle delivery/CO2 removal mask either was not in place or correctly positioned, or in the case of the CO 2 removal pump system malfunction. Thus, the maximum recoded zero for EtCO 2 was approximately the background concentration of CO 2 in the sealed HEF (749 mmHg × 0.0038 = 2.846 mmHg). Later, we were able to simulate this pattern of zero shift with the particle delivery/CO 2 removal mask incorrectly positioned and this explained the unexpected CV of the MINIMUM variable for the EtCO 2 signal. See Figure 4 for the EtCO 2 tracing of that original test in trend format.
CV for the slopes of phase II and III, for both measurements, showed that these two variables are also the ones with the largest physiologic variability even in the steady state. Thus, they have the potential to be considered as   sibly due to the psychological effect of the limited space in the HEF, which might affect the variability of the breathing pattern.
In the group of EtCO 2 signal variables, we found that the CV for RMS was smaller than for the MEAN, because RMS has been calculated as the true RMS and better represents the "average" value of expired CO 2 than the MEAN value obtained by the standard equation. The true RMS, in this case, better approximates mixed-expired CO 2 (P E CO 2 ) per one breath or over the time that the EtCO 2 signal was "averaged". The P E CO 2 and arterial partial pressure for CO 2 (P a CO 2 ) are used to calculate dead space to tidal volume ratio (V D /V T ).

Conclusion
We have described a customized, inexpensive and feasible hardware and software implementation of standard capnography, as an adjunct to our human exposure facility, which is used in air pollution studies. Our hardware and software setup for acquisition and analysis of EtCO 2 wave signal was demonstrated as reliable and stable. We would have preferred that the selected capnograph monitor have an analog output, so we could avoid digital to analog signal conversion. The instrument was meant to be used in standard pre-hospital or hospital environments, therefore the manufacturer had not considered that option. However, extending the use of capnography into research labs like ours, where we carry out air pollution human exposure studies, has been long overlooked. Using capnography in the way we described, we may be able to detect and better characterize the effect of exposure to air pollution on cardiopulmonary system, particularly on lung ventilation and perfusion. We plan to use this technique in our on-going and subsequent controlled human exposure studies as a physiologic outcome measure.

Authors' contributions
KZL initiated and designed the study, designed capnographic hardware & software setup, performed data analysis and interpretation, and drafted the manuscript. BU contributed to overall aspects of the study and specifically in statistical analysis, results interpretation and manuscript drafting. MF contributed in technical design modifications and conducted all experimental procedures and measurements. MEF contributed to study design and implementation with valuable clinical input and comments on physiologic aspects of capnography, while FS, the principal investigator, contributed valuable discussion and suggestions throughout this project. All authors read and approved the final manuscript.