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[Response of isozyme and stress indexes of Coptis chinensis to UV-B radiation].

OBJECTIVE
To study the physiological mechanism of anti-stress of Coptic chinensis and provide theoretical basis for its cultivation and promoting its quality.


METHODS
Different degrees of the range of time and intensity of UV-B radiation were set in the experiment. Used the technique of polyacrylamide gelatin vertical board electrophoresis (PAGE) to analyse the isozyme and related stress index.


RESULTS
The isoenzymic bands of SOD1 (Rf = 0.125), SOD2 (Rf = 0.312), CAT1 (Rf = 0.428), POD3 (Rf = 0.290), POD4 (Rf = 0.636) were induced by UV-B radiation after 3 hours, with the increase of the time of UV-B radiation, those isoenzymic bands was going to vanish or became unclear. Moreover, isoenzymic bands of CAT1 (Rf = 0.428), POD3 (Rf = 0.290) disappeared in advance under heavy intensity of UV-B radiation. Furthermore, the contents of MDA, soluble sugar, proline were higher dramatically than those of control group under UV-B radiation. However, excluding the increases of proline in UL group, the content of MDA, soluble sugar, proline of other groups commenced to decrease slowly and isoenzymic bands of soluble protein increase after 7 hours of UV-B radiation.


CONCLUSION
The increase of the expression of antioxidase isozyme, accumulation of soluble sugar, soluble protein and other antioxidase matter is induced by the short-time UV-B radiation, which can protect Coptis chinensis from being harmed by UV-B radiation. However, regulation system of Coptis chinensis are broken, metabolism is disordered, the bands of antioxidase isozyme vanish or weaken, the bands of soluble protein are increased and widened, these phenomenon is caused by 7 hours of UV-B radiation.