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Phytochemical Screening of Plant Extracts and GC-MS Analysis of n-Hexane Extract of the Leaves of Cassia alata Linn

The purpose of this investigation was to undergo the preliminary phytochemical screening of the plant extracts and identify the presence of pharmacologically bioactive constituents in the leaves of Cassia alata by using gas chromatography-mass spectrometry (GC-MS) technique. The preliminary phytochemical screening of different leaf extracts of Cassia alata revealed the presence of various phytochemical compounds such as terpenoids, steroids, flavonoids, phenolic compounds, quinones, carbohydrates, tannins and alkaloids. Qualitative and quantitative determination of different biologically active compounds from the crude n-hexane extract using gas chromatography-mass spectrometry disclosed 20 compounds with varying amounts where main components were identified as palmitic acid (26.65%), stearic acid (14.27%), (E)-9-octadecadienoic acid (11.40%), erucylamide (8.34%), 1,19-eicosadiene (5.15%), stigmasterol (4.68%), linoleic acid (4.06%), vitamin E (3.97%), methyl palmitate (3.93%) and methyl 11-octadecenoate (3.32%). The compounds identified through this investigation may be responsible for any of the pharmacological properties of Cassia alata and could be of considerable interests for the development of new drug leads.