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[Experiences with the macrophage adherence inhibition test within the framework of preparing tumor-associated antigens from human breast cancers].

We try to prepare tumor-associated antigens from human mammary carcinomas by the use of several biochemical methods and to detect the biologic activity of these antigens with the macrophage adherence inhibition assay. The diagnostic relevance of the macrophage adherence inhibition assay was to improve by optimal enrichment of the tumor-associated antigens. By the use of this test system an enrichment of tumor-associated antigens was not detectable. Our results show, that the macrophage adherence inhibition assay because of its low sensitivity (53%) and the insufficient reproducibility can not be used as test system for tumor immunity in human cancer.