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Use of a Coulter counter to detect discrete changes in cell numbers and volume during growth of Escherichia coli.

Escherichia coli could be detected at a concentration of 105 cells/ml. Growth curves of the organism grown in filtered tryptone soya broth were made by taking viable and Coulter counts. Both curves were similar in shape and varied little during 10 h growth. Cell volume and volume distribution were seen to vary considerably however, volume reaching a stable minimum value of 0·61 μm3 after 8 h. The Coulter Counter was considered to be potentially useful for detecting significant bacteriuria.