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c‐myc Gene expression is localized to the myocyte following hemodynamic overload in vivo

Expression of the proto‐oncogene c‐myc increases in the hemodynamically overloaded heart, but expression by cardiac myocytes has not been shown. To address this issue, right ventricular overload was induced in cats by pulmonary artery banding. Expression of c‐myc and α‐skeletal actin mRNA were determined by Northern analysis. Immuno‐reactive Myc protein was identified by histochemical staining.