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HIGH-DENSITY ARRAY OF SINGLE CELL TRAPS FOR HIGH-THROUGHPUT IMAGING OF CALCIUM DYNAMICS IN RESPONSE TO OXIDATIVE STRESS

Stochasticity in gene expression, protein or metabolite levels contributes to cell-cell variations. The analysis of these variations could lead to a better understanding of cellular processes and drug responses, but require single cell analysis techniques. To this end, we developed a new microfluidic platform for high-throughput capture (in about a minute) with low shear and imaging of thousands of single cells. This platform is compatible with upstream components for complex cell stimulation patterns. Imaging of calcium dynamics in response to oxidative stress with this platform reveals variable phenotypic responses within clonal populations.