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Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation

Neutrophils are recognised to play a pivotal role at the interface between innate and acquired immunities following their recruitment to inflamed tissues and lymphoid organs. While neutrophil trafficking through blood vessels has been extensively studied, the molecular mechanisms regulating their migration into the lymphatic system are still poorly understood. Here, we have analysed neutrophil-lymphatic vessel interactions in real time and in vivo using intravital confocal microscopy applied to inflamed cremaster muscles. We show that antigen sensitisation of the tissues induces a rapid but transient entry of tissue-infiltrated neutrophils into lymphatic vessels and subsequent crawling along the luminal side of the lymphatic endothelium. Interestingly, using mice deficient in both TNF receptors p55 and p75, chimeric animals and anti-TNFα antibody blockade we demonstrate that tissue-release of TNFα governs both neutrophil migration through the lymphatic endothelium and luminal crawling. Mechanistically, we show that TNFα primes directly the neutrophils to enter the lymphatic vessels in a strictly CCR7-dependent manner; and induces ICAM-1 up-regulation on lymphatic vessels, allowing neutrophils to crawl along the lumen of the lymphatic endothelium in an ICAM-1/MAC-1-dependent manner. Collectively, our findings demonstrate a new role for TNFα as a key regulator of neutrophil trafficking into and within lymphatic system in vivo.

Neutrophils have classically been considered to be prototypical short-lived and terminally differentiated phagocytes involved in innate immune responses following their rapid recruitment at site of infections and acute inflammation [1][2][3] . Compared to other leukocytes, their life span does not exceed 1-5 days in the circulation 4 though several cytokines such as GM-CSF 5,6 , bacteria-derived products 7 , hypoxia 8 or their migration through the blood vessel walls 9,10 can significantly expand their life expectancy both in vivo and in vitro. Until recently, neutrophils were thought to end their life in inflamed tissues by apoptosis before being engulfed by other phagocytic cells to limit and resolve inflammation 11,12 . However, 45 years ago, neutrophils were detected within the lymphatic system 13 . It was suggested that this response represented a way for neutrophils to recirculate back into the blood vasculature; and their role in the lymphatic system was thus largely neglected. Recently, however, accumulating evidence has shown that the role of neutrophils spans beyond the basic but fundamental immunological processes of innate immunity 14 . Specifically, neutrophils can actively participate in the regulation of adaptive immunity following exposure to pathogens and antigens sensitisation [14][15][16][17] by means of antigen presentation or cytokine release for stimulating T-and B-lymphocytes [18][19][20][21][22] as well as DCs 23 .
While their role in regulating adaptive immunity is now well documented, few studies have investigated the mechanisms associated with neutrophil migration into the lymphatic vasculature. A clue to this phenomenon was first suggested through neutrophil localisation in the draining lymph nodes (dLNs) within the capsula and carrying fluorescently-labelled antigens or pathogens from the sites of antigen-sensitisation or infection 15,16,24 . These results indicated their provenance from tissue-associated lymphatic capillaries via afferent lymphatic vessels. Furthermore, antigen-bearing neutrophils trafficking to dLNs preceded the influx of other professional antigen-presenting cells such as DCs and macrophages 25 ; and blocking their entry or depleting mice in circulating neutrophils reduced T-cell proliferation in vivo 26 , confirming a critical role for these leukocyte in the development of adequate adaptive immune responses. Recently, neutrophils have also been shown to migrate directly into the LNs from the blood circulation via high endothelial venules (HEVs). The molecular signature of these migratory responses is however unclear; and there are currently conflicting data in the literature regarding the chemotaxis pathway involved in this phenomenon. A role for CCR7 in neutrophil migration into the LNs of immunised animals was first demonstrated 27 , while other studies have implicated the CXCR4:CXCL12 axis and Sphingosine-1-Phosphate 26,28 . Leukocyte integrins and selectins have also been implicated in neutrophil trafficking to LNs 28 , though their exact contribution to cell migration through afferent lymphatic vessels near sites of inflammation in vivo is still unclear.
Despite these seminal but conflicting reports, further investigations are required to fully understand the mechanisms associated with this response. Here we provide evidence for the involvement of TNFα in the trafficking of neutrophils into but also within the lymphatic vasculature in vivo. Specifically, using a mouse model of antigen sensitisation and cytokine-induced inflammation of the cremaster muscle, we demonstrate that TNFα orchestrates both neutrophil migration into lymphatic vessels in a CCR7-dependent manner and their subsequent crawling along the lymphatic endothelium in an ICAM-1/MAC-1-dependent manner. Collectively, the present findings identify a previously unknown role for TNFα in orchestrating sequential interactions of neutrophils with tissue-associated lymphatic vessels during the acute inflammatory response of antigen sensitisation; and highlight TNFα as a potential target for the manipulation of neutrophil regulation of adaptive immune responses within the lymphatic system.

Results
TNFα promotes neutrophil migration into lymphatic vessels of murine cremaster muscles.
Neutrophil migration into the lymphatic system has been described in models of infections and immunisation sensitisation 15,16,24,27 , but the mechanisms associated with this response are not fully understood and somewhat controversial. For this purpose, we studied neutrophil-lymphatic vessel interactions in vivo using a mouse model of cremaster muscle inflammation, allowing the direct visualisation in 3-and 4-dimensions of cell-cell interactions by high-resolution confocal microscopy. Whole-mount cremaster tissues of mice immunostained for LYVE-1 and PECAM-1/VE-Cadherin, showed the presence of a unidirectional network of lymphatic vessels with characteristic blind-ended lymphatic capillaries and collecting afferent vessels made up of oak-leaf shaped lymphatic endothelial cells (LEC) ( Supplementary Fig. S1) as previously described in other tissues 29,30 . Following tissue-stimulation with exogenous TNFα , neutrophils were rapidly detected in the lumen of lymphatic vessels (Fig. 1a). Detailed analysis of TNFα -stimulated tissues demonstrated a time-dependent migration of neutrophils out of blood vessels post TNFα -administration (Fig. 1b). This response was associated with a rapid and transient migration of neutrophils into lymphatic vessels at 8 hrs post-inflammation (Fig. 1c), as well as into the cremaster muscle draining lymph nodes (dLNs) (Fig. 1d). We then went on to analyse in vivo and in real time the dynamics of neutrophil-lymphatic vessel interactions in the cremaster muscle of the neutrophil reporter LysM-GFP mice upon TNFα -stimulation. For this purpose, in vivo fluorescent-immunostaining with non-blocking anti-PE-CAM-1 and/or a non-inhibitory dose of anti-LYVE-1 mAbs was applied to the tissues to visualise endothelial cells and lymphatic vessels, respectively, to allow the tracking of GFP high neutrophils responses into the lymphatic vasculature by intravital confocal microscopy. With this technique, neutrophils were seen to migrate rapidly (4.5 ± 0.6 min) through LECs ( Fig. 1e and Videos 1 and 2). Furthermore, we observed that following their entry into the lymphatic vessels, intravasated neutrophils were firmly attached to the LECs and were crawling along the luminal surface of the lymphatic endothelium. (Fig. 1f and Video 3). Analysis of neutrophil crawling dynamics showed that 63.7 ± 5.7% of the neutrophils crawl along the luminal surface of the LECs in the direction of the lymphatic flow at a speed of 4.3 ± 0.2 μ m/min; while the few neutrophils venturing against the natural direction of lymphatic flow showed a reduced crawling speed, displacement length and straightness ( Fig. 1g-j).
Collectively, these results demonstrate that the cytokine TNFα induces the migration of neutrophils into lymphatic vessels and promotes their crawling along the luminal aspect of LECs in vivo.
TNFα controls the entry of neutrophils into lymphatic vessels following antigen sensitisation.
Having observed the efficacy of exogenous TNFα in inducing neutrophil migration into lymphatic vessels, we next investigated the potential role of endogenous TNFα in this response in a model of antigen sensitisation. non-blocking anti-PECAM-1 mAb (blue)]. The right panel images illustrate a time-lapse series of 2 μ m-thick cross-sections along the z-plane (dotted-yellow arrow) showing the migration of two neutrophils (Cell-1 & Cell-2) into the lymphatic vessel. (f) Representative 3D-reconstructed still image of a lymphatic vessel from a TNFα -stimulated cremaster tissue of a LysM-GFP mouse and immunostained with an anti-LYVE-1 mAb (red) in vivo. Neutrophil crawling path (colour-coded line) and directionality (white arrow) is shown on the image. The bottom panel images are a series of high magnification cross-sections of the main image at indicated timepoints illustrating the continuous attachment of the neutrophil to the lymphatic endothelium. (g) Percentage of neutrophils crawling in the afferent direction (flow) or in the opposite direction (anti-flow). Speed (h), directionality (i) and straightness (j) of neutrophils crawling in the afferent (flow) or opposite direction (antiflow) of the cremaster lymphatic vessels. Data are expressed as mean ± SEM from 5-12 animals per group (at least 5 independent experiments). For the crawling parameter analysis, a total of 63 cells were quantified from 8 mice. Statistically significant differences between stimulated and unstimulated treatment groups are indicated by asterisks: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Significant differences between responses at different time points are indicated by hash symbols: # P < 0.05; #### P < 0.0001. Bar = 10 μ m.
For this purpose, we first analysed the migration response of neutrophils into the dLNs of mice subjected to skin inflammation with an emulsion of an antigen in Complete Freund's Adjuvant (CFA+ Ag), reproducing the classical immunisation procedure. Injection of CFA+ Ag induced a time-dependent increase in the number of neutrophils in the dLNs (namely inguinal and lumbar LNs) as compared to non-dLN (i.e. axillary LNs) (Fig. 2a). This model was then extended to the cremaster muscle to look at neutrophil-lymphatic vessel interactions in vivo. Quantification of neutrophil migration responses through blood and into lymphatic vasculatures showed a time-dependent increase in the number of neutrophils (Fig. 2b,c), both peaking at 8 hrs post-inflammation. This response was associated with an increase in neutrophil infiltration of the dLNs of the cremaster muscles but not of non-dLNs (Fig. 2d). Because of the low incidence of cells (less than 1% of total LN-infiltrated leukocytes as observed by flow cytometry) and to exclude from the analysis the increase of circulating neutrophils due to CFA+ Ag-induced neutrophilia (Fig. S2), we performed detailed analysis of whole-mount dLNs immunostained for HEVs (blood vasculature), LYVE-1 (lymphatic vasculature) and MRP14 (neutrophils) by confocal microscopy. We showed that ~60% of neutrophils (Fig. 2e) at 8 hrs post-inflammation were within LYVE-1+ vessels. At 16 hrs (e) Quantification of neutrophil localisation in the dLNs of mice stimulated intra-scrotally with CFA (8 or 16 hrs) or with PBS (control), and as analysed by confocal microscopy. Data are represented as percentages of neutrophils present in the HEV, LYVE-1+ vessels and in the stroma of the LNs. Data are expressed as mean ± SEM of N = 5-12 animals (~10 images per cremasters for confocal microscopy) per group from at least 5-10 experiments. Statistically significant differences between stimulated and control groups or between WT and TNFRdbKO mice are indicated by asterisks: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Significant differences between other groups are indicated by hash symbols: ## P < 0.01; #### P < 0.0001.   post-inflammation, only ~25% of the neutrophils were found in this location while ~58% of the cells were found in the LN stroma. Overall, only a minority of the neutrophils were found within the HEVs at these time-points. These data suggest that the early and rapid migration of neutrophils into dLNs may occur via afferent lymphatics.
Interestingly, CFA+ Ag-stimulation of cremaster muscles induced a rapid release of TNFα in tissues from 8 hrs onward and reaching the maximum response at 16 hrs post-inflammation (Fig. 3a). Furthermore, mice depleted in resident phagocytes (i.e. macrophages) following the local delivery of clodronate liposomes exhibited a reduction in TNFα release as compared to control liposome-treated animals (Fig. 3b). To investigate the functional role of endogenous TNFα during CFA+ Ag-inflammation, neutrophil migration responses were quantified in mice deficient in both TNFR p55 and P75 receptors (TNFRdKO mice). These studies showed that while neutrophil extravasation into tissues was similar between WT and TNFRdbKO mice (Supplementary Figs S3a and Fig. 3c), neutrophil migration into cremaster lymphatic vessels was reduced by 84% and 75% in KO animals at 8 hrs and 16 hrs post-inflammation, respectively (Supplementary Fig. S3b and Fig. 3d). This response was associated with a reduced (~64%) neutrophil-infiltration of the dLNs (Fig. 3e). In order to address the origin of cell type responding to the endogenous release of TNFα during inflammation and promoting neutrophil migration into the lymphatic vessels, we generated chimeric animals. For this purpose, lethally irradiated WT mice were reconstituted with bone marrow hematopoietic cells from either WT or TNFRdbKO animals before being subjected to antigen sensitisation ( Supplementary Fig. S4). Similarly to full TNRFdbKO mice, the neutrophil migration response into the lymphatic system was impaired in chimeric animals exhibiting WT vasculature and tissue-resident cells but neutrophils deficient in TNFRs, as compared to the control group; while extravasation through blood vessels was not affected (Fig. 3f-h).
Together these data demonstrate that endogenous TNFα directly primes the leukocytes to trigger the migration of neutrophils into tissue-associated lymphatic vessels post-antigen sensitisation in vivo.

TNFα promotes CCR7-dependent migration of neutrophils into lymphatic vessels in vivo.
Having identified a role for endogenous TNFα in controlling the entry of neutrophils into lymphatic vessels, we then went on to decipher the molecular mechanism involved and more specifically which chemokine/chemokine axis was associated with this response. We first investigated the role of CXCL1 on neutrophil trafficking into the lymphatic vasculature in vivo, as the human equivalent of this chemokine has been shown recently to promote neutrophil migration through a monolayer of lymphatic endothelial cells in vitro 31 . However, local delivery of anti-CXCL1 blocking antibody did not affect the migration of neutrophil into the lymphatic vasculature of inflamed cremaster muscles in vivo (Supplementary Fig. S5a-c). We then went on to analyse the role of CXCR4:CXCL12 axis shown previously to promote the entry of neutrophils into the lymphatic system in different inflammatory models 26,28 . Similarly to CXCL1-blockade, local injection the CXCR4 specific inhibitor AMD3100 did not significantly influenced the capacity of neutrophils to migrate into the lymphatic vessels upon antigen sensitisation ( Supplementary Fig. S5d-f). In fact, analysis of blood circulating leukocytes by flow cytometry showed that while CFA+ Ag-inflammation induced neutrophilia in both AMD3100-and vehicle-treated mice as compared to unstimulated animals (Fig. S5g), CXCR4 surface expression was down-regulated with the inflammation (Fig. S5h). Collectively, these results suggest that the chemotactic axes CXCL12:CXCR4 and CXCL1:CXCR1/2 do not play a significant role in neutrophil recruitment into the lymphatic vessels during the acute inflammatory response as induced by CFA+ Ag in vivo.
Finally, the potential involvement of the CCL21/CCR7 axis, widely linked with DC/T-cell migration into the lymphatic system 32 was then explored in our model despite conflicting studies regarding the role of CCR7 in neutrophils 26,27 . The expression of CCR7 on blood-born, cremaster tissue-infiltrated and dLN-infiltrated neutrophils, was first examined by flow cytometry post CFA+ Ag-induced inflammation (Fig. 4a). WT blood neutrophils did not express CCR7 on their surface, we could detect intracellular stores of the molecule. Interestingly, neutrophils isolated from CFA+ Ag-inflamed cremaster muscles showed a small but significant expression of CCR7 on their cell surface. Interestingly, this upregulation of CCR7 on tissue-infiltrated neutrophils was absent in TNFRdbKO as compared to WT littermates (Fig. 4b). Furthermore, stimulation of mouse blood neutrophils with low concentrations of TNFα in vitro induced the surface expression of CCR7 ( Supplementary Fig. S6). Having found that tissue-infiltrated neutrophils up-regulate CCR7 on their cell surface, we hypothesised that this chemokine receptor may mediate the migration of neutrophils through lymphatic vessels of the cremaster muscles. To address this, we initially looked at neutrophil migration responses in both WT and CCR7KO animals following TNFα stimulation. Interestingly, while neutrophil extravasation into tissues was not affected (Fig. 4c), trafficking of neutrophils muscle and dLNs (16 hrs post-inflammation) was assessed by confocal microscopy. (f) Number of extravasated neutrophils in inflamed cremaster muscles of WT and CCR7KO mice. (g) Number of intravasated neutrophils in cremaster lymphatic vessels of WT and CCR7KO mice. (h) Neutrophil number in the cremaster dLNs of WT and CCR7KO mice. (i) Number of neutrophils recruited to the cremaster muscles from lethally irradiated WT animals receiving bone marrow transplant from either WT or CCR7KO donor mice. (j) Number of neutrophils within cremaster lymphatic vessels post-CFA+ Ag-stimulation from lethally irradiated WT animals receiving bone marrow transplant from either WT or CCR7KO donor mice. (k) Neutrophil number in the cremaster dLNs from lethally irradiated WT animals receiving bone marrow transplant from either WT or CCR7KO donor mice. Data are expressed as mean ± SEM of N = 7-12 animals per group (from at least 5 independent experiments). Statistically significant differences between stimulated/specific mAb and unstimulated treatment/ isotype control groups are indicated by asterisks: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Significant differences between responses in WT vs. CCR7KO animals (or between different tissues) are indicated by hash symbols: # P < 0.05; ## P < 0.01; ### P < 0.001; #### P < 0.0001. into cremaster muscle lymphatic vessels was completely inhibited in CCR7KO animals as compared to WT animals (~97% suppression) (Fig. 4d). With regard to their infiltration into cremaster dLNs, TNFα induced an increase of neutrophils compared to control group (Fig. 4e). However, in TNFα -stimulated CCR7KO mice, the number of neutrophils into the dLNs was comparable to the levels found in unstimulated animals. Similarly, while neutrophil extravasation in response to CFA+ Ag was unaffected in CCR7KO mice (Supplementary Fig. S3a and Fig. 4f), neutrophil migration into lymphatic vessels was suppressed by ~86% and 75% in CCR7KO animals as compared to WT mice in this model at 8 and 16 hrs post-inflammation, respectively (Supplementary Fig. S3b and  Fig. 4g). Furthermore, and in contrast to WT animals, the level of dLN-infiltrated neutrophils was not increased in CCR7KO mice after stimulation with CFA+ Ag (Fig. 4h). Of note, CCR7KO animals exhibited ~25 times more LN-infiltrated neutrophils than WT mice in unstimulated conditions, a response related to a higher expression of CXCL12 in the LNs of CCR7KO animals as compared to WT mice ( Supplementary Fig. S7), suggesting a compensatory mechanism during the development of these GM mice. Furthermore, using the specific inhibitor of CXCR4 (receptor for CXCL12), AMD3100, we could reduce the number of neutrophils present in the LNs of CCR7KO animals (Supplementary Fig. S7). To overcome the abnormal trafficking of neutrophils into the LNs in CCR7KO animals, we generated chimeric animals by injecting lethally irradiated WT mice with bone marrow cells from CCR7KO animals (or WT bone marrow cells as control). This resulted in the generation of animals exhibiting CCR7KO circulating neutrophils in a WT environment. Similarly to a full KO, chimeric exhibiting CCR7KO neutrophils showed a reduced migration of these leukocytes (~76% reduction) into the lymphatic vasculature of the cremaster muscle upon antigen challenge while neutrophil recruitment from the blood into the tissue was similar to the control chimeric group (Fig. 4i-j). Interestingly, CCR7KO-neutrophil chimeric animals also showed a reduced number of neutrophils infiltrating the dLNs (~62%) as compared to control littermate (Fig. 4k); suggesting that neutrophils from CCR7KO donor cells are not able to migrate efficiently into the LNs of the WT recipient mice.
Collectively, these data demonstrate that TNFα mediates CCR7-dependent migration of neutrophils into afferent lymphatic vessels. . Statistically significant differences between the staining of isotype control and anti-ICAM-1 Abs treated groups are indicated by asterisks: *P < 0.05; ****P < 0.0001. Significant differences between unstimulated and inflamed groups are indicated by hash symbols: ## P < 0.01; #### P < 0.0001. Bar = 50 μ m.Results are expressed as mean ± SEM of N = 4-9 mice (1-2 vessels analysed/mouse for intravital confocal microscopy, each mice is a single experiment). Significant differences between blocking antibodies treated and control groups are indicated by *P < 0.05; ***P < 0.001; ****P < 0.0001. Significant differences between other groups are indicated by # (P < 0.05). Bar = 30 μ m. TNFα also controls the crawling of neutrophils along the luminal side of afferent lymphatic endothelium. Using intravital confocal microscopy we observed that TNFα -induced inflammation results in neutrophils crawling along the lymphatic endothelium ( Fig. 1f and Video 3). Similarly to TNFα -stimulation, CFA+ Ag led to neutrophil crawling along the lumen of LECs (Video 4). The majority of these leukocytes moved in the direction towards collecting lymphatic vessels/lymph flow (67.8 ± 3.8%) at a speed of 5.8 ± 0.2 μ m/min and with a straightness index of 0.40 ± 0.04; while cells going against lymph flow direction had reduced speed and directionality of movement ( Fig. 5 and Video 5). Interestingly, the favoured directionality of neutrophils was associated with the establishment of a gradient of CCL21 within the lymphatic vessel in the direction of the lymph flow during inflammation ( Supplementary Fig. S8). Furthermore, when an anti-TNFα blocking antibody was injected 4 hrs post-CFA + Ag-stimulation, the few neutrophils present within the lymphatic lumen completely lost their directional motility. Cells did not show any preferential direction of migration but exhibited instead a meandering crawling path associated with reduced speed and directionality as compared to isotype control treated animals.
These data suggest that, during the acute phase of the inflammatory response to CFA+ Ag, TNFα controls the directional crawling of neutrophils within the lymphatic vessels.

ICAM-1 mediates TNFα-induced neutrophil crawling within lymphatic vessels.
To investigate the molecular mechanisms of neutrophil intraluminal crawling in lymphatics, we studied the role of ICAM-1, an adhesion molecule known to support neutrophil crawling along both the luminal 33 and abluminal 34 surfaces of blood vessels. In initial studies we analysed ICAM-1 expression on cremaster lymphatics under basal and inflamed conditions by immunostaining. Both TNFα and CFA+ Ag induced an up-regulation of ICAM-1 expression in vivo as compared to vehicle-treated animals (Fig. 6a,b). Interestingly, when CFA+ Ag-stimulated cremaster muscles were pre-treated with an anti-TNFα blocking antibody, the expression of ICAM-1 on lymphatics was reduced as compared to the control antibody-treated group (Fig. 6c). To further assess the role of ICAM-1 in neutrophil crawling behaviour within the lymphatic vessels, we performed functional assays using local administration (injected 4.5 hrs post-inflammation) of functional blocking antibodies against ICAM-1 or its leukocyte binding partner, the integrin MAC-1 in CFA+ Ag-stimulated cremaster muscles of LysM-GFP mice. Both anti-ICAM-1 and anti-MAC-1 mAbs impaired neutrophil intraluminal crawling within lymphatics as compared to an isotype control Ab (Fig. 7a,b and Video 6/7/8). Specifically, blocking ICAM-1 or MAC-1 resulted in a ~50% reduction in the speed of crawling, displacement length and straightness within the luminal side of lymphatic endothelium (Fig. 7c-e). In contrast, interstitial migration was not affected by these treatments. Overall, while the time and route of delivery of the blocking antibodies had no effect on leukocyte extravasation from blood vessels and migration into lymphatic vessels, respectively (Fig. 7f,g), blocking the intraluminal crawling of neutrophils within lymphatic vessels resulted in a reduction in the number of neutrophils infiltrating the dLNs (Fig. 7h).
Collectively, the present findings identify ICAM-1 as an adhesion molecule that mediates neutrophil crawling along the lymphatic endothelium and identify TNFα as a key regulator of neutrophil directional motility within lymphatic vessels in vivo.

Discussion
It is now well established that neutrophils contribute to the shaping of the adaptive immunity against many foreign antigens or infectious agents following their rapid migration into the lymphatic system 15,19,35 . However, the mechanisms of his migratory behaviour are poorly understood. In the present study we have identified a previously unknown role for endogenous TNFα in orchestrating neutrophil trafficking into and within the lymphatic vasculature of inflamed tissues in vivo. Specifically, we demonstrate that in a mouse model of antigen sensitisation, endogenous TNFα directly instructs the neutrophils to migrate into the lymphatic vessels in a strictly CCR7-dependent manner and also induces their subsequent directional crawling along the lumen of the lymphatic endothelium as mediated by ICAM-1 up-regulation on lymphatic endothelial cells. The new mechanisms of TNFα action on neutrophil-lymphatic interactions are summarised in Fig. 8.
In initial studies aimed at investigating TNFα -induced neutrophil trafficking into extravascular tissues, we noted that tissue infiltrated neutrophils could rapidly migrate into lymphatic vessels. These studies were then extended to a model of antigen sensitisation characterised by the generation of endogenous TNFα . This reaction was also associated with rapid neutrophil migration into lymphatic vessels, a response that was impaired in mice deficient in both TNF receptors p55 and p75. Interestingly, TNFα did not appear to mediate neutrophil extravasation from blood vessels into tissues, indicating a specific role for TNFα in driving neutrophil motility isotypic control (CTL, top panel), anti-ICAM-1 (middle panel) or anti-MAC-1 (bottom panel) mAbs. (b) The graphs show the crawling paths of neutrophils in the X & Y planes of the lymphatic vessels from CTL Ab, anti-ICAM-1 and anti-MAC-1 mAbs treated groups. (c-e) The effect of anti-ICAM-1 and anti-MAC-1 blocking antibodies on neutrophil migration parameters (i.e. interstitial and intraluminal crawling) was quantified and compared to the responses obtained with an isotype CTL mAb. The three graphs show the mean speed (c), directionality (d) and straightness (e) of neutrophils crawling. A total of 280 cells were analysed. The numbers of neutrophils in the interstitial tissue (f) and inside the lymphatic vessels (g) were quantify by confocal microscopy. (h) Neutrophil infiltration of cremaster dLNs was also quantified at the end of the experiment.Results are expressed as mean ± SEM of N = 4-9 mice (1-2 vessels analysed/mouse for intravital confocal microscopy, each mice is a single experiment). Significant differences between blocking antibodies treated and control groups are indicated by *P < 0.05; ***P < 0.001; ****P < 0.0001. Significant differences between other groups are indicated by # (P < 0.05). Bar = 30 μ m.
into lymphatic vessels but not through blood vasculature. Furthermore, using chimeric animals we showed that TNFα acts directly of the leukocytes to induce this neutrophil migration response.
In the present study, we clearly demonstrated that the TNFα -driven migration of neutrophils into lymphatic vessels is strictly CCR7-dependent. The role of CCR7 in neutrophil recruitment to the lymphatic system was demonstrated both in a mouse model of intradermal immunisation and in human neutrophils by Beauvillain et al. 27 . In accordance with their study, we only detected intracellular stores of CCR7 in murine blood neutrophils, but not on their cell surface. However our results shows that neutrophils recruited into inflamed tissues up-regulated CCR7 on their surface as compared to blood circulating cells. A study by Eruslanov et al. have shown similar up-regulation of CCR7 on human neutrophils in a tumour model 36 , though the mechanism and physiological consequences of this response in human is still unclear. Our current data suggests priming of neutrophils for enhanced chemokine receptor expression. While GM-CSF and IL-17 have been shown to prime human neutrophils to migrate toward the chemokines CCL21 and CCL19 in vitro 27 , we did not detect the generation of these cytokines in the inflamed cremaster muscles at any time-point analysed ( Supplementary Fig. S9). However, TNFRdbKO tissue-infiltrated neutrophils did not show an increase in CCR7 expression on neutrophils in vivo; while low concentrations of TNFα promoted the surface expression of CCR7 on murine blood neutrophils in vitro. Altogether these data suggest a predominant role of TNFα in this response and may highlight the importance of the origin of the cells analysed (e.g. bone marrow vs blood, tissue-infiltrated vs naïve cells, murine vs. human leukocytes). Interestingly, we observed that CCR7KO animals exhibited a high basal level of neutrophils within the LNs of naïve animals as compared to WT mice; and inflammation of the cremaster muscles with TNFα or following antigen sensitisation did not increase further the number of neutrophils found in the LNs. This abnormal trafficking of neutrophils into the LNs of these mice could be related to a higher expression of CXCL12 in naïve CCR7KO dLNs as compared to WT dLNs. CXCR4 expression was similar in both genotypes, however, CXCR4 antagonist treatment reduced significantly the number of these leukocytes infiltrating CCR7KO dLNs, while their migration into the afferent lymphatics was not affected (Supplementary Fig. S7). These data support the hypothesis that CXCR4 signalling contributes to neutrophil homing in LN through HEV 28 .
Since the role for CCR7 in neutrophils trafficking has been contentious to date, we have also investigated other chemokine/chemokine receptor axes in this phenomenon, such as the CXCL12/CXCR4 axis. This axis was shown recently to be involved in neutrophil trafficking to the lymphatic system in a model of bacterial infection with S. aureus 26 . However, our data demonstrate that CXCR4 blockade did not significantly inhibit neutrophil migration into cremaster lymphatic vessels upon CFA+ Ag-stimulation ( Supplementary Fig. S5). Such differences between their study and ours could be related to the model and inflammatory pathway involved: S. aureus infection may

Figure 8. Schematic diagram illustrating the dual mechanisms of action of TNFα leading to the trafficking of neutrophils into and within the lymphatic vasculature upon acute inflammation in vivo.
During the acute inflammatory response of the tissue following antigen sensitisation, endogenous TNFα release primed the freshly recruited neutrophils. This cytokine allow these leukocytes to be attracted to the lymphatic vessels in a CCR7 dependent manner (intravasation). Furthermore, endogenous TNFα also stimulate the lymphatic endothelium to express ICAM-1 on their surface, allowing the neutrophils present in the lymphatic vessels to adhere and crawl along the luminal side in the correct direction toward the flow of the vessel.
preferentially activates the toll-like receptor 2 (TLR2) pathway in vivo [37][38][39][40] . Recently, TLR2 was shown to be associated with CXCR4 in lipid rafts of monocytes to induce signalling 41 , a response not yet investigated for neutrophils. In contrast, the TLR4 pathway is involved in M. tuberculosis infections, LPS or CFA stimulation 42 ; and TLR4 ligands can induce TNFα release more rapidly than TLR2 agonists in vitro 43 . Finally, both TNFα 44 and TLR4 activation 45 have been shown to down-regulate the expression of CXCR4 on neutrophils, rendering them less responsive to CXCL12 stimulation. This supports our observation of a similar down-regulation of CXCR4 expression by neutrophils in our inflammatory model (Supplementary Fig. S5). Furthermore, CXCR4 is highly expressed on a subclass of ageing neutrophils rather than healthy mature cells 46 . Altogether, these observations may indicate that different sub-populations of neutrophils or their pathway of activation might be important for the molecular axis used for their migration into the lymphatic system.
Another study has shown that the potent neutrophil-chemoattractant CXCL8 was up-regulated in TNFα -stimulated human dermal endothelial cells in vitro; and promotes neutrophil migration through a monolayer of human LECs 31 . In the mouse system, LECs isolated from mouse skin showed an upregulation of CXCL1 gene upon inflammation 47 . However, our in vivo model of TNFα -induced neutrophil trafficking into lymphatic vessels, clearly indicated the predominant role of CCR7 in this phenomenon. Furthermore local treatment with an anti-CXCL1 blocking mAb did not affect the capacity of neutrophils to enter the lymphatic system upon inflammation in vivo. These data highlight differences between in vivo and in vitro models. Indeed, several studies pointed to the importance of the microenvironment for the LECs to retain their specific lymphatic characteristic that are lost in culture such as the capacity to generate CCL21 48,49 . Similarly to a mouse model of contact hypersensitivity 47 , we did not observed a change in total CCL21 expression between naïve and inflamed animals ( Supplementary Fig. S8). We however noticed that lymphatic vessels exhibited higher expression of CCL21 as compared to the interstitial tissue with the establishment of a gradient that could direct the neutrophils toward the vessels in vivo. The formation of a gradient of CCL21 driving the migration of DCs within the LNs has been recently reported in the literature 50 .
Having identified TNFα as a key signal for promoting neutrophil trafficking to the lymphatic vessels, the potential involvement of this cytokine in mediating other neutrophil-lymphatic vessel interactions was investigated. TNFα is the prototypical pro-inflammatory cytokine playing a key role in many immune responses such as cell recruitment and leukocyte activation. It is also involved in numerous pathological conditions and autoimmune disorders such as rheumatoid arthritis, lupus, psoriasis and atherosclerosis and there is now considerable evidence for successful use of TNFα blockers for the treatment of certain chronic inflammatory conditions. However, anti-TNFα therapy is associated with a heightened risk of serious infections and poor vaccination responses in patients 51 . Furthermore, the mechanism through which these drugs work is not fully known; though suppressing leukocyte recruitment and activation is considered to be a principle mode of action 52 . In our acute model of cremasteric inflammation however, we neither genetic deficiency for TNFα signalling nor antibody blockade inhibited neutrophil recruitment to the tissue, suggesting different roles for TNFα in acute vs chronic inflammation. During physiological conditions, TNFα can prime blood vascular endothelial cells (BECs) to present both adhesion molecules and chemoattractants in order to induce leukocyte migration. Specifically, TNFα has been shown to up-regulate ICAM-1 expression on BECs both in vivo and in vitro, an adhesion molecule essential for neutrophil directional crawling during their recruitment through blood vessels 33,34 . Lymphatic endothelial cells (LECs) express very low levels of ICAM-1 in uninflamed condition, but can up-regulate this molecule upon TNFα -stimulation in both humans 53,54 and mice (Fig. 6). The exact role of ICAM-1 in leukocyte migration into the lymphatic system is however controversial: in vitro antibody blockade inhibits both adhesion and transmigration responses of DCs through cultured human LECs 53 , and in vivo ICAM-1 have been reported to mediate crawling of DCs along the lymphatic endothelium 55 . In contrast, DC interstitial migration was not affected in mice exhibiting leukocyte-specific deletion of ICAM-1-ligand integrins 56 . In the present study, we have observed that neutrophils crawl along the luminal surface of the lymphatic endothelium following a local inflammatory stimulus. This response was associated with a formation of a gradient of CCL21 within the vessel ( Supplementary Fig. S8e). This gradient could explain why the majority of neutrophils crawl along in the direction of the lymphatic flow. Interestingly, Russo et al., have recently demonstrated that in vitro, murine LECs generate a gradient of CCL21 when exposed to low sheer stress and that this gradient was responsible for the directionality of crawling of DCs 57 . In our model, the neutrophil crawling response (as well as ICAM-1 upregulation) was blocked when mice received an injection of an anti-TNFα blocking antibody. Furthermore, local blockade of ICAM-1 or MAC-1 inhibited this crawling response while interstitial migration of the leukocytes and their capacity to enter the lymphatic vessels were unaffected. Overall, the local injection of blocking antibodies inhibiting neutrophil-lymphatic endothelium luminal interactions resulted in a reduction of neutrophil numbers infiltrating the LNs, highlighting potential and effective targets to manipulate the role of neutrophils in adaptive immune responses in vivo.
In conclusion, the data presented here provide an insight into the mechanisms underlying neutrophil trafficking into and within lymphatic vessels of inflamed tissues. Specifically, we have unravelled a predominant role for endogenous TNFα in orchestrating both CCR7-dependent migration of neutrophils into afferent lymphatics and ICAM-1-dependent crawling on the luminal surface of lymphatic endothelium during the acute phase of the inflammatory response as induced by antigen sensitisation. Overall, our findings identify TNFα as a new molecular regulator of neutrophil migration into the lymphatic system that may provide the opportunity for the development of improved immunisation protocols but also highlight a new potential mechanism of action -and limitations -for anti-TNFα therapy.

Animals.
Male mice (8-12 weeks) wild-type (WT, Charles Rivers), CCR7 knockout (CCR7KO, JAXLab), LysM-EGFP ki (LysM-GFP), LysM-EGFP ki × CCR7 ko (LysM-GFP/CCR7KO) (all on a C57BL/6 background) and TNFRdb knockout (TNF receptors p55 and p75 double knockout mice, JAXLab) mice were used for these experiments. Only heterozygote LysM-GFP animals exhibiting fluorescent myeloid cells (neutrophils comprising the highest percentage of GFP high cells) were used for this study with the permission of T. Graf (Albert Einstein College of Medicine, Bronx, NY). These animals were provided by M. Sperandio (Ludwig Maximilians University, Munich, Germany) and bred in-house in individually ventilated cages; and facilities were regularly monitored for health status and infections. Chimeric mice deficient in leukocyte TNFR p55 and p75 or were generated by lethal irradiation of C57BL/6 WT mice (5.5 Gy twice, 4 h apart) and injection of bone marrow cells (1.5 × 10 6 cells/recipient i.v.) from TNFRdbKO mice. C57BL/6 WT littermates receiving WT bone marrow were used as controls. The phenotype of blood circulating neutrophils in chimeric animals was then assess by flow cytometry (Fig. S4). Similar protocol was used for the generation of chimeric animals exhibiting CCR7KO leukocytes following the injection of donor LysM-GFP/CCR7KO bone marrow cells into lethally irradiated WT recipient animals. Depletion of tissue-resident macrophages of the cremaster muscles was achieved by 3 consecutive i.s. injections of clodronate liposomes (Encapsula NanoSciences LLC; 250 μ g/mouse, 20 hrs apart) prior to the induction of inflammation. With this protocol we achieved ~85.9% of depletion as quantified by flow cytometry (data not shown). Confocal Microscopy. Intravital confocal microscopy. LysM-GFP mice were stimulated with i.s. injection of either TNFα or CFA+ Ag and the inflammatory response was allowed to develop for 4 (TNFα ) to 6 hrs (CFA+ Ag) before visualisation of the response by intravital confocal microscopy. In some experiments, CFA+ Ag-stimulated animals were pre-treated with a local (i.s.) injection of the following blocking antibodies 90 to 120 min before surgery: anti-TNFα or rat IgG1κ isotype control mAbs (50 μ g/mouse), anti-ICAM-1/anti-MAC-1 mAbs or rat IgG2b isotype control mAbs (10 μ g/mouse) along with non-blocking dose of an anti-LYVE-1 mAb (2 μ g/mouse, Alexa555 conjugated) and/or a non-blocking anti-PECAM-1 mAb (2 μ g/mouse, Alexa647 conjugated) to label the lymphatic and blood vasculatures, respectively. Thirty minutes before surgery, mice were sedated with i.p. injection of ketamine (100 mg/kg) and xylazine (10 mg/kg). Following surgery, cremaster muscles were imaged with a Leica SP5 or SP8 confocal microscope 58 for another 90 min with a superfusion of warm Tyrode's solution. Images were acquired every minute with the use of a 20 × water-dipping objective (NA:1.0) with sequential scanning of different channels at a resolution of 1024 × 700 pixels in the x × y plane and 0.7 μ m steps in z-direction. 4D confocal image sequences were then analysed offline using IMARIS software (Bitplane, Switzerland), enabling the dynamic interaction of neutrophils with lymphatic vessels be observed, tracked, and quantified as previously described 34 .
Confocal microscopy on fixed tissue. Following immunostaining, the cremaster muscles were imaged with a Leica SP8 confocal microscope with the use of a 20 × water-dipping objective (NA:1.0). Images of post-capillary venules and lymphatic vessels (at least 6 vessels per tissue) were attained with the use of sequential scanning of different channels at every 0.52 μ m of tissue depth at a resolution of 1024 × 470 and 1024 × 800 pixels in the x × y plane, respectively. This resolution of pixels correspond to a voxel size of 0.24 × 0.24 × 0.5 μ m in x × y × z. Post-capillary venules and lymphatic vessels were imaged at a zoom factor of × 1.9 and × 1.2, respectively. Quantification of neutrophil transmigration and intravasation into lymphatic vessels were analysed with the 3D-reconstructing image processing software IMARIS. Transmigrated neutrophils were defined as the number of neutrophils present in the extravascular tissue across a 300 μ m blood vessel segment and within 50 μ m from each side of the venule of interest. Data was expressed as the number of neutrophils per volume of tissue. Intravasated neutrophils were defined as the number of neutrophils present inside the lymphatic vessels and data were expressed as the number of neutrophils per given volume of lymphatic vessel quantified by IMARIS Software by creating an isosurface representing exclusively the LYVE-1 positive lymphatic endothelium. To assess ICAM-1 and CCL21 expression on lymphatic vessels, specific primary antibodies or control isotype-matched antibodies were injected i.s. 1 hr prior to the exteriorisation of the cremaster muscles; and following the analysis of tissues by confocal microscopy, the mean fluorescence intensity (MFI) of the staining for molecule of interest was determined using IMARIS software on the LYVE-1 isofurface as determined by IMARIS software. Intensity profiles for CCL21 expression along a certain distance (10 μ m away from or 100 μ m within the lymphatic vessel) was performed using Image J. For the LNs, halved samples were imaged with a Leica SP8 confocal microscope with the use of a 10 × water-dipping objective (NA:0.3). Images (12 images per pair of LNs per mouse) were obtained with the use of sequential scanning of different channels at every 5.8 μ m of tissue depth at a resolution of 1024 × 1024 pixels in the x × y plane, corresponding to a voxel size of 0.91 × 0.91 × 5.8 μ m in x × y × z. Quantification of neutrophil recruitment into the inguinal LNs were analysed with the 3D-reconstructing image processing software IMARIS. Recruited neutrophils were defined as the number of neutrophils per volume of tissue, excluding (unless specified) the blood circulating neutrophils present in HEVs.
Flow cytometry. Whole blood and single cell suspension from (collagenase + DNAse)-digested cremaster muscles and LNs of CFA+ Ag-stimulated WT and CCR7KO animals, were fluorescently labelled with conjugated antibodies against CD45.2, Ly6G, CD11c, CD3ε , CCR7, CXCR4, TNFRp55, TNFRp75 or the appropriate isotype control antibodies (0.2-2 μ g/ml, various fluorochromes) and DAPI (for viability) for at least 1 hr at 4 °C. Viable leukocytes were identified by FSC and SSC characteristics and CD45.2 positive and DAPI negative staining. Neutrophils were identified based on Ly6G high staining. In some experiments, blood leukocytes where incubated at 37 C in RPMI medium (supplemented with 10% FCS and 2 mM of L-Glutamin) with various concentration of TNFα (1, 10 or 100 ng/ml) for 4 hrs in the presence of 50 μ M of Nystatin (Sigma, an endocytosis inhibitor) prior to immunofluorescence staining. Samples were analysed using a BD LSR-Fortessa (BD Biosciences) and FlowJo analysis software (Treestar). In some studies the intracellular expression of CCR7 was performed using the Cytofix/Cytoperm kit (BD) according to the manufacturer's recommendations.
ELISA. Snap frozen cremaster tissues from WT mice stimulated with CFA+ Ag were transferred into screw-top tubes containing homogenising beads along with 500 μ l homogenising buffer (1% Triton ™ X-100, 1% protease inhibitor, PBS) prior to being placed in a high-throughput tissue homogeniser, Precellys ® 24 (Precellys, Derbyshire, UK), for 3 cycles of 20 s homogenisation at 6500 r.p.m with 40 s rest between each cycle. Homogenised samples were then frozen at − 80 °C for 1 h before being thawed and centrifuged for 5 min at 10,000 g using a tabletop centrifuge. The supernatant was taken and used to quantify the release of TNFα , GM-CSF, IL-17, CCL21, CCL19 and CXCL12 by ELISA (eBioscience, Hatfield, UK or R&D Systems, UK) according to the manufacturer's protocol.
Statistical analysis. Data are presented as mean ± S.E.M per mouse. Significant differences between multiple groups were identified by one-way analysis of variance (ANOVA), followed by Newman-Keuls Multiple