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Effect of low pH treatment and agents causing mitotic delay on intracellular pH of sea urchin eggs.

The intracellular pH of unfertilized eggs and those at early prophase was measured using labeled dimethyloxazolidinedione. The pH was not altered by agents which induce mitotic delay but was decreased slightly by lowering extracellular pH which reduces mitotic delay. The evidence suggests that the effect of the agents causing mitotic delay may not be mediated by changes in intracellular pH, but the reduction by low pH of mitotic delay may be mediated by it.