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High-frequency Floral Bud Regeneration from Petal Segment Cultures of Sinningia speciosa Hiern

The influence of gibberellin and cytokinin on the regeneration of floral bud from petal segments of Sinningia speciosa Hiern in vitro was studied here. Two types of regeneration were regeneration of floral buds only (designated B F ), and regeneration of both floral and vegetative buds (designated B F+V ). The capacity of floral bud regeneration was influenced by light, the size of the floral bud and exogenous gibberellin (GA 3) and cytokinin in the media. In MS basic medium containing 1.0 mg/L GA3, the addition of 6-benzyladenine (BA, 0.5 mg/L) sig- nificantly increased the frequency of total (B F +B F+V ) flower bud formation to 100%. The culture was in darkness, the frequency of B F regeneration was up to 93.4%. The highest percentage of B F was 86.7% formed in petal seg- ments from 7 mm floral buds in diameter. Morphological changes in tissue structure of petal segment cultures were observed at several stages (0 to 30 d) under the light microscopy.