{'fraction_non_alphanumeric': 0.041791044776119404, 'fraction_numerical': 0.030845771144278607, 'mean_word_length': 5.023952095808383, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
[Study on macrophages infected with Mycobacterium tuberculosis H37Ra in vitro].

AIM
To study the production of nitric oxide and secretion of cytokines after infection of macrophages with Mycobacterium tuberculosis H37Ra.


METHODS
24 hours after infection of RAW264.7 cells with Mycobacterium tuberculosis H37Ra, the production of NO and H(2);O(2); as well as the secretion levels of IL-12 and TNF-α in the supernatants of culture were determined by Griess method, chemical method and ELISA assay respectively. The expression of IL-12 and TNF-α mRNA in macrophages was detected by reverse transcription polymerase chain reaction (RT- PCR).


RESULTS
Macrophages infected with Mycobacterium tuberculosis H37Ra produced effectively more NO, H(2);O(2);, and enhanced the release of IL-12, TNF-α and the expression of IL-12, TNF-α mRNA (P<0.05).


CONCLUSION
Mycobacterium tuberculosis H37Ra can induce the production of more nitric oxide and cytokines which play important roles in the host immune response.