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Study on Extraction of Shikimic Acid from Pine Needles of Pinus Elliottii Engelm by Means of Microwave Pretreatment

The aim was to provide scientific basis for development and utilization of pinus elliottii engelm resources.As the raw material, pines needles of pinus elliottii were moistened first by vaporization medium. After that, the needles were processed in advance by microwave to change tissues of needles, and finally, the Shikimic acid was extracted from the needles in hot water. Meanwhile, the extraction method is compared with traditional one in the paper. As a result, the perfect technological conditions were gained for extraction of Shikimic acid by microwave preprocessing. The method is: moistening pine needles within 15 minutes in 70% ethanol water which was 1.6 times weight of needles, then, processing the needles for 60 seconds by 70W microwave. The processing time should increase 30 seconds every 5-gram material added. Finally, the extraction lasted over 30 minutes adding 25-time hot water at 80°C. Seven eighths of extraction time by microwave preprocessing is shortened compared by the traditional extraction method, and extraction ratio for Shikimic acid raises by 35.8%.