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Nucleotide sequence of a cDNA encoding a Trypanosoma cruzi acidic ribosomal PO protein: a novel C-terminal domain in T. cruzi ribosomal P proteins.

Trypanosoma cruzi ribosomal proteins are antigenic in chronic human T. cruzi infections (1). Immunoscreening of a Xgtl 1 bloodstream trypomastigote cDNA library (2) allowed the isolation of a recombinant, Try-PO, containing a 979 bp long polyadenylated cDNA that encoded the partial sequence of a T.cruzi ribosomal PO protein. Two oligonucleotides were synthesized corresponding to nucleotides 1-20 of the T.cruzi miniexon (sense strand), and 62-84 (non coding strand) of the Try-PO cDNA. They were used to amplify the 5' end of the ribosomal PO protein mRNA by RNA-PCR. The amplified 227 bp cDNA (5'PO) was sequenced. Together, the 5'PO and Try-PO cDNAs span the complete ribosomal PO mRNA sequence. It encodes for a 321 amino acid long peptide, with a molecular weigth of about 34.500, and an estimated pI of 5,66. It is 4 residues longer than the corresponding Drosophila and human counterparts (3, 4), and 9 residues longer than the Saccharomyces cerevisiae L1O protein (5). Its carboxy C-terminal molecular T. cruzi acidic ribosomal P