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Chemical composition, ultrastructure and some serological properties of lipopolysaccharides from Leptotrichia buccalis.

Lipopolysaccharide (LPS) was extracted with aqueous phenol from Leptotrichia buccalis strains L 11, ATCC 14201 and ATCC 19616. Virtually all the LPS was found in the water phase. LPS could also be extracted with phenol/chloroform/petroleum ether, but not with cold trichloroacetic acid. All LPS preparations contained D-glycero-D-manno-heptose, galactose, glucose, glucosamin, n-dodecanoate, 3-hydroxy-n-tetradecanoate and phosphorus. In addition LPS from L11 contained 2-keto-3-deoxyoctonate and an unidentified aldose. LPS from L11 was serologically distinct from LPS of ATCC 14201 and ATCC 19616. LPS from the two latter strains crossreacted.