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[Quantitative determination of peimisin and total alkaloids in Fritillaria taipaiensis of different growing stage].

OBJECTIVE
To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage.


METHODS
High performance liquid chromatography coupled with evaporative light scattering detector was employed, and peimisine was separated and quantified upon an Agilent Extend C18 (250 mm x 4.0 mm, 5 microm), using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL min(-1); UV spectrophotometry was used for the determination of total alkaloids.


RESULTS
During from two to three years period, the content of peimisine in F. taipaiensis increased with growing stage, from four to six years, it decreased with growing stage; From two to four years, the content of total alkaloids in F. taipaiensis increased with growing stage, from five to six years, it decreased with growing stage.


CONCLUSION
It is suitable to harvest F. taipaienssi of four years growing stage.