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Preparation and evaluation of effect on Escherichia coli and Staphylococcus aureus of radiolabeled ampicillin‐loaded graphene oxide nanoflakes

Ampicillin is a one of effective antibiotics against Gram‐positive and Gram‐negative bacteria. This study aimed to label ampicillin‐loaded graphene oxide nanoflake (AMP‐GO) with 99mTc and evaluate of its in vitro binding to Staphylococcus aureus and Escherichia coli. Firstly, ampicillin was loaded into graphene oxide nanoflake prepared. AMP‐GO was characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) techniques, and the amount of loaded ampicillin onto GO was determined by UV‐Vis absorption spectroscopy. AMP and AMP‐GO were labeled with 99mTc using stannous chloride reducing agent. Labeling efficiency of 99mTc‐AMP‐GO was found to be 97.66 ± 2.06%. 99mTc‐AMP‐GO has higher binding efficiencies to both S. aureus and E. coli than 99mTc‐AMP. 99mTc‐AMP‐GO could be promising candidate as agent infection nuclear imaging. Furthermore, in vivo studies of 99mTc‐AMP‐GO with infected rats are planned to be performed.