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Laser-Raman Spectroscopy Of Biological Molecules

In the years immediately following its discovery in 1928, the Raman effect gained wide use among chemists and physicists in the solution of structural problems that could not be handled then by other available techniques. The simplicity of photographic spectroscopy compared with the more difficult procedures of infrared spectroscopy in that period contributed to this state of affairs. Consequently, when automatically recording infrared instruments became available in the late 1940's, the situation was reversed and the Raman effect was relatively little used. Today, however, technical advances in Raman instrumentation, particularly the advent of the laser, have elevated Raman spectroscopy once more to a par with infrared methods.