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Imaging flow cytometry reveals a subset of TdT negative T‐ALL blasts with very low forward scatter on conventional flow cytometry

Studies in T‐cell acute lymphoblastic leukemia (T‐ALL) have shown that leukemic blast populations may display immunophenotypic heterogeneity. In the clinical setting, evaluation of measurable residual disease during treatment and follow‐up is highly dependent on knowledge of the diversity of blast subsets. Here, we set out to evaluate whether variation in expression of the blast marker, TdT, in T‐ALL blasts could correspond to differences in morphometric features.