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Quantitation of Binding of Factor VIII Antigen to Concanavalin A

Summary. This study establishes a convenient method for screening plasma samples for abnormalities of the carbohydrate content of the factor VIII (FVIII) molecule. A radioimmuno‐electrophoretic technique has been developed to quantitate the percentage binding of FVIII‐related antigen (VIII‐Ag) to the lectin concanavalin A (Con A). Plasma samples were electrophoresed through a strip of agarose containing Con A into agarose containing a mixture of unlabelled anti‐FVIII and 125I‐anti‐FVIII where precipitant lines formed, the height of which was dependent upon the degree of VIII‐Ag binding to Con A in the first gel. Using this system reduced binding of VIII‐Ag to Con A was found in the plasma of 12 patients with moderate classical von Willebrand's disease (vWd), while the Con A binding of six haemophilia A patients fell within the normal range. The VIII‐Ag in normal cryoprecipitate showed increased % binding to Con A while the VIII‐Ag remaining in the cryo‐supernate demonstrated reduced Con A % binding.