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Effect of Exercise on Mitochondrial Regulation in Tumorigenic Testes of Aged Fischer 344 Rats

Tumors are associated with dysregulated mitochondrial‐mediated apoptosis and impaired mitochondrial metabolism, and the incidence of tumors increases with age. While exercise has been shown to reduce mitochondrial‐mediated apoptosis and improve mitochondrial bioenergetics in various tissues, its effect on aged tumorigenic testes is unknown. Thus, we examined whether mitochondrial apoptotic markers and mitochondrial content differed in, 1) young control testes (YC; 6m; Fischer 344; n=4 group), 2) old control non‐tumorigenic testes (OC; 22–24m; Fischer 344), 3) old tumorigenic testes (OT), 4) old tumorigenic exercise‐trained (OT‐E; 10 weeks treadmill training) testes. OT and OT‐E testes mass were similar but significantly larger (1.5‐fold) than YC and OC. Mitochondrial content was modestly reduced in OC compared to YC but was elevated similarly in OT and OT‐E testes compared to YC. AIF, a pro‐apoptotic factor, was not different in YC and OC testes but was significantly elevated by 60–70% in OT and OT‐E testes. Bax:Bcl‐2 ratio, an apoptotic index, was similar in YC and OC testes, while both OT and OT‐E testes tended to be elevated compared to YC. Our findings suggest paradoxically that both mitochondrial content and mitochondrial apoptotic susceptibility were not altered by age alone, but enhanced by tumorigenesis and that exercise did not attenuate these mitochondrial abnormalities.