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Application of Organoclay Nanoparticle in Low‐Density Polyethylene Films for Packaging of UF Cheese

Polymer/clay nanocomposites are a group of hybrid materials made from layered silicates of clay dispersed in the polymer matrix with a layer thickness in nanometre. The ability of organoclay nanoparticle including cloisite 15A, cloisite 20A and cloisite 30B incorporated into a low‐density polyethylene (LDPE) polymeric matrix was investigated in order to inhibit the growth of coliform bacteria in ultra‐filtrated cheese. The number of surviving cells of coliforms was decreased by 2.21 log cfu/g after 4 weeks storage at 4 ± 0.5°C, while pure LDPE showed a reduction of only 0.759 log cfu/g. LDPE film composed of 3% cloisite 15A, 3% cloisite 20A, with no cloisite 30B nano‐filler was found as optimum point by combined design approach in design‐expert analysis. The resulting film showed coliform load decreased by 2.05 log cfu/g in the optimum point, which was in line with Japanese industrial standard (JIS Z 2801: 2000) about antibacterial effect on coliform bacteria. A suitable microbial model for bacteria growth reduction was also suggested. Copyright © 2016 John Wiley & Sons, Ltd.