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Varietal Descriptors for the Distinction of Underutilized Varieties of Sechium edule (Jacq) Swartz

Sechium edule (Jacq.) Sw. (Cucurbitaceae) is a species native to Mexico and Central America. The collection, characterization, and evaluation of accessions maintained in genebanks is essential for the conservation of this species. However, there are no specific varietal descriptors that differ from those used in a phenetic approach and are adapted to international registration guidelines to help distinguish, improve, cluster, and protect intraspecific variants of common use and those obtained by breeding. Therefore, 65 morphological descriptors (qualitative and quantitative) were evaluated in 133 accessions obtained from Mexico, Guatemala, and Costa Rica located in the National Germplasm Bank of S. edule in Mexico. These characteristics were observed to be phenetically stable for five generations under the same agroclimatic conditions. In addition, an analysis of amplified fragment length polymorphism (AFLP) was applied to 133 samples from a set of 245 accessions. According to the multivariate analysis, 26 of the 65 descriptors evaluated (qualitative and quantitative) enabled differentiation of varieties of S. edule. The AFLP analysis showed a high level of polymorphism and genetic distance between cultivated accessions and their corresponding wild ancestor. The variations in S. edule suggest that the morphological characteristics have differentiated from an essentially derived initial edible variety (ancestral original variety), but unlike other cucurbits, there is no evidence of the ancestral edible for Sechium since the seed is unorthodox and there are no relicts.


Introduction
The characterization and intellectual protection of vegetables, with the aim of ex situ conservation, basic research, and genetic improvement programs, are established using a series of morphological, chemical, genetic, and physiological descriptors that fulfill the requirements of the distinction, uniformity, and stability (DUS) test, by applying the standards of the International Union for the Protection of New Varieties of Plants (UPOV), and, recently, using digital phenotyping to discriminate varietal groups [1,2]. According to UPOV [3], "a variety will be considered distinct if it differs from any other variety whose existence is commonly known, and it may be considered homogeneous if it is stable
The analysis of hierarchical conglomerates denoted the distribution of evaluated accessions based on the qualitative characteristics and attempted to determine their relative importance as explanatory variables. In this sense, the semi-partial correlations defined the role played by each characteristic, aside from their apparent prominence in the phenotype, by means of grouping and assigned them a causality. Figure 1 shows the formation of a main group encoding a value of 0.09, which suggested a high similarity index between accessions for the evaluated characteristics. Different subgroups (0.06, 0.04, and 0.04) were derived from the main group, generating a series of intermediate clusters and, within them, most of the varietal groups in the inferior interval were estimated between values of 0.021 and 0.018. Qualitative variables, by their ordinal nature and considering the characteristic of distinction, avoid subjectivity and facilitate interpretation and classification because uniform intervals between measurements are unnecessary (e.g., small, medium, high, higher, or light, moderate, heavy). However, quantitative variables may acquire any value within a specified interval of values, such as dimensions of the fruit, for example, allowing that there may always be a value between any two values. Therefore, quantitative variables cannot be subjected to a criterion of order, such as variations of fruit color. are unnecessary (e.g., small, medium, high, higher, or light, moderate, heavy). However, quantitative variables may acquire any value within a specified interval of values, such as dimensions of the fruit, for example, allowing that there may always be a value between any two values. Therefore, quantitative variables cannot be subjected to a criterion of order, such as variations of fruit color. The above mentioned is relevant because of its statistical value. The outstanding variables were demonstrated to be characteristics that may be influenced by the environment; however, they continued to be important even though they could be quantitatively transformed (e.g., color, shape, spines, and dimensions), since they acquired continuity and stability in their values. Another important aspect of these variables was their relative value in the statistical load of the component, which allowed the establishment of relationships with other evaluated parameters, for example, pubescence, venation order, shape, furrows, or ribs, thus increasing the degree of continuity despite environmental influence. These variables showed unalterable characteristics that were homogeneous and uniform from generation to generation.
The values obtained from the annual measurement of morphological characteristics were lower since the standard error was very low. These results were due to measurements being taken in the month of June (beginning of flowering) and ending in late July in order to reduce crossbreeding and obtain self-pollinated samples. Some varieties began to bear fruit before emitting lateral vines (precocity), making it easier to obtain samples without interbreeding.

Quantitative Characteristics
Regarding the quantitative variables, principal component analysis (PiCo) demonstrated that the first six components explained up to 78.6% of the variability of the accessions (45.6, 12.5, 5.9, 5.6, 4.6, and 4.4). The values obtained for Pearson's correlation coefficients (PC) between two quantitative random variables was independent of their measurement scale, as shown in Table 2. The variables that contributed to the variation were: PC1: FSW (fruit size width), FSD (fruit size depth), MT (mesocarp thickness), SSL (seed size length), and SSW (seed size width); PC2: FS (flower size), RaL (rachis length), RaD (rachis diameter), and SSD (seed size depth); PC3: BL (bud length) and RL (receptacle length) The above mentioned is relevant because of its statistical value. The outstanding variables were demonstrated to be characteristics that may be influenced by the environment; however, they continued to be important even though they could be quantitatively transformed (e.g., color, shape, spines, and dimensions), since they acquired continuity and stability in their values. Another important aspect of these variables was their relative value in the statistical load of the component, which allowed the establishment of relationships with other evaluated parameters, for example, pubescence, venation order, shape, furrows, or ribs, thus increasing the degree of continuity despite environmental influence. These variables showed unalterable characteristics that were homogeneous and uniform from generation to generation.
The values obtained from the annual measurement of morphological characteristics were lower since the standard error was very low. These results were due to measurements being taken in the month of June (beginning of flowering) and ending in late July in order to reduce crossbreeding and obtain self-pollinated samples. Some varieties began to bear fruit before emitting lateral vines (precocity), making it easier to obtain samples without interbreeding.

Quantitative Characteristics
Regarding the quantitative variables, principal component analysis (PiCo) demonstrated that the first six components explained up to 78.6% of the variability of the accessions (45.6, 12.5, 5.9, 5.6, 4.6, and 4.4). The values obtained for Pearson's correlation coefficients (PC) between two quantitative random variables was independent of their measurement scale, as shown in Table 2. The variables that contributed to the variation were: PC1: FSW (fruit size width), FSD (fruit size depth), MT (mesocarp thickness), SSL (seed size length), and SSW (seed size width); PC2: FS (flower size), RaL (rachis length), RaD (rachis diameter), and SSD (seed size depth); PC3: BL (bud length) and RL (receptacle length)  Figure 1 shows clearly defined clusters in two large groups with origins in the interval close to 0.3 and 0.4 of the correlations based on quantitative variables, indicating wide variability among these parameters reflected as two large groups with smaller subgroup formation. Based on the values from Table 2, it is assumed that a linear relationship-positive as well as negative-may exist between variables without reaching perfect dependence. The International Union for the Protection of New Varieties of Plants (UPOV) states that the distinction, uniformity, and stability (DUS) test is required to recognize a variety and thus attain legal protection. Morphological characteristics are the basis of this test, complying with the following criteria: (a) they must be clearly different from all collections of reference varieties; (b) they are maintained through different environments and times; and (c) they must be stable through reproduction. Figures 2 and 3 show a wide variety of morphological characteristics that might be due to high heterogeneity in the lineage of the groups. These characteristics substantially increase the number of differences between accessions and thus a greater possibility of distinguishing varietal independence. Based in these results, Table 3 demonstrates proposed guidelines to conduct the test for distinctness, uniformity, and stability in S. edule (Jacq.) Sw.   Figure 1 shows clearly defined clusters in two large groups with origins in the interval close to 0.3 and 0.4 of the correlations based on quantitative variables, indicating wide variability among these parameters reflected as two large groups with smaller subgroup formation. Based on the values from Table 2, it is assumed that a linear relationshippositive as well as negative-may exist between variables without reaching perfect dependence. The International Union for the Protection of New Varieties of Plants (UPOV) states that the distinction, uniformity, and stability (DUS) test is required to recognize a variety and thus attain legal protection. Morphological characteristics are the basis of this test, complying with the following criteria: (a) they must be clearly different from all collections of reference varieties; (b) they are maintained through different environments and times; and (c) they must be stable through reproduction. Figures 2 and 3 show a wide variety of morphological characteristics that might be due to high heterogeneity in the lineage of the groups. These characteristics substantially increase the number of differences between accessions and thus a greater possibility of distinguishing varietal independence. Based in these results, Table 3 demonstrates proposed guidelines to conduct the test for distinctness, uniformity, and stability in S. edule (Jacq.) Sw.  Quantitative and qualitative morphological characteristics of biological variants that allow varietal distinction of Sechium edule. Pubescence (a1,a2); color and striation of the guide (a3,a4); shape, pubescence, and leaf venation (a5-a7).

AFLP Analysis
In 133 individuals belonging to S. edule, 228 AFLP-type molecular markers were used to analyze kinship relationships between the different cultivated and wild varietal groups. Cluster analysis was run in Rstudio using the factoextra and FactoMineR packages. The distance matrix of the AFLP markers was constructed using the ade4 package and Dice coefficients. The optimal number of groups was calculated using the NbClust package, which performed a consensus of 30 indices, resulting in a majority rule, thus determining eight groups as ideal to describe the distance matrix. Group I highlighted varietal complexes with small fruit, such as albus minor, nigrum conus, and nigrum minor. Group II contained 75% of the amarus sylvestris accessions. Group III included all of the wild accessions of S. edule. Group IV was made up of various varietal complexes, making an in-depth discussion difficult. Groups V and VI were characterized by individuals with smooth fruit, such as virens levis and albus levis. Group VII combined the genotypes of the varietal complexes nigrum xalapensis and nigrum minor, although a subgroup with individuals of the albus levis varietal group was observed. Group VIII was very diverse with elements of different varietal complexes, as in groups III and IV. It was possible to make cuts in the dendrogram to create more groups with concordance, which was supported in the consulted literature and based on the varietal complexes of S. edule (Figure 4).
Groups I to VIII show the grouping of S. edule varieties, where certain genotypes predominated due to; however, they were not pure groups, since there are no speciation differences. They still form groups that share genetic characters even when morphologically they have differences in color, shape, size, spines, etc. This is common in groups of plants with manipulation through their life history and process of domestication and cultivation.
The main coordinate analysis showed 76.58% of the total variation, in which main coordinate 1 explained 62.8% and main coordinate 2 explained 13.78%. Using the GenAlEx program, genetic distances between populations of the 133 individuals were analyzed using AFLP molecular markers. S. edule var. amarus sylvestris and the wild types were differentiated from the rest of the accessions, together with S. edule var. albus spinosum, of which only one accession was analyzed. Sechium compositum (included as a possible external taxon; however, this wild genotype is morphologically related to the intraspecific variations of S. edule, with no apparent barriers to interbreeding) clustered close to the varietal groups virens levis, nigrum xalapensis, and spiny green chayote. In general, the varietal complexes of S. edule tended to cluster in the center of the graph. However, the nigrum levis, albus minor, and nigrum conus varietal groups were located very close to each other and closest to the wild type materials because they shared common morphological characteristics (small size for albus minor and dark green color for albus minor, nigrum levis, and nigrum conus) ( Figure 5). complexes nigrum xalapensis and nigrum minor, although a subgroup with individuals of the albus levis varietal group was observed. Group VIII was very diverse with elements of different varietal complexes, as in groups III and IV. It was possible to make cuts in the dendrogram to create more groups with concordance, which was supported in the consulted literature and based on the varietal complexes of S. edule (Figure 4). specific variations of S. edule, with no apparent barriers to interbreeding) clustered close to the varietal groups virens levis, nigrum xalapensis, and spiny green chayote. In general, the varietal complexes of S. edule tended to cluster in the center of the graph. However, the nigrum levis, albus minor, and nigrum conus varietal groups were located very close to each other and closest to the wild type materials because they shared common morphological characteristics (small size for albus minor and dark green color for albus minor, nigrum levis, and nigrum conus) ( Figure 5).

Discussion
The morphological characteristics that facilitated the visual differentiation of genotypes of intraspecific complexes of S. edule were evident (Figures 2, 3 and 6), even when the correlation with genetic diversity was low, because the plants share the same origin [17,18]. However, some genotypes displayed sufficient differentiation with regards to the type closest to the wild type, suggesting that the morphological descriptors in Table 3 will allow the differentiation, organization, and identification of chayote in activities of conservation, research, and genetic improvement with greater ease of follow-up than the characteristics previously described [19,20].
Doust et al. [21] indicated that domestication, as an evolutionary process, generates changes in phenotypes, but such changes are not due to individual causes and tend to be associated with the environment, while certain phenotypic changes can take place rapidly, particularly when the histories of the populations studied are divergent. This could be the case in this study, where there were as many divergent histories as origins of the genotypes analyzed.
Gross and Olsen [22] mentioned that both convergent phenotypical evolutions, referring to the appearance of the same trait in independent evolutional lineages, and parallel phenotypical evolution, explained as the appearance of the same trait closely related or potentially intercrossed between lineages, take place in cultivated plants. This could have happened in the varietal groups of S. edule. These authors related this, among other causes, to the selective introgression of a single allele through cultivation. In contrast with many cultivated plants, such as cereals in which traits that are a product of domestication have many antecedents [23], the domestication history of S. edule is relatively recent and mixed with environmental adaptations, generating new traits in both cases [7].
The plants used in this study have been included in BANGESe since 2005 and are native to Costa Rica, Guatemala, and the Mexican states of Chiapas, Oaxaca, Puebla, Morelos, Hidalgo, Michoacán, Nayarit, San Luis Potosí, State of Mexico, and Veracruz ( Figure 6). Most of the accessions came from backyard areas and in many cases there was reproductive isolation determined by consumer preference. The sample size included 133 live accessions obtained through the choice proposed [31] from a population of 245 accessions in the germplasm bank (supplementary material). Figure 6. Fruit characteristics (shape, color, size, furrows, and spines) of the varieties of Sechium edule regarding their wild predecessor (a7). Medium, small, and very small fruit: (a1-a6) (nigrum levis, albus levis, albus dulcis, nigrum conus, albus minor, nigrum minor), big fruit (a8-a12) (nigrum maxima, nigrum xalapensis, virens levis, nigrum spinosum, albus spinosum) (According to [11]).
Because previous biochemical, morphological, and anatomical studies showed stability of the distinctive features of S. edule, the number of individuals per accession [2] was reduced and the number of generations evaluated was increased to five. The successive evaluation of the accessions was carried out annually for five generations, harvesting fruit from two chosen plants per accession at physiological maturity, and the plants generated from these fruit were replanted in an open area adjacent to the Genebank. Given the previous results of parsimony and stability, the sample size (n = 133) was maintained in each generational evaluation. To reduce the risk of pollen contamination, successive fruit were selected from the first third of the plant. Multiple plants were generated and identified in order to identify the typical characteristics of the corresponding varietal group. The morphological evaluation was carried out annually for five generations, measuring each year, adhering to the protocol of the Sechium germplasm Figure 6. Fruit characteristics (shape, color, size, furrows, and spines) of the varieties of Sechium edule regarding their wild predecessor (a7). Medium, small, and very small fruit: (a1-a6) (nigrum levis, albus levis, albus dulcis, nigrum conus, albus minor, nigrum minor), big fruit (a8-a12) (nigrum maxima, nigrum xalapensis, virens levis, nigrum spinosum, albus spinosum) (According to [11]).
The ecological and cultural conditions of traditional agriculture have helped to preserve the genetic diversity of several economic species and influence the response of current cultivated forms to processes of empirical selection in different regions. In the case of Sechium, many accessions came from rural backyards with different origins. Additionally, the preferences of consumers affect the preservation of the type of chayote, such as preferences regarding the color of the fruit. For example, fruit of var. nigrum (dark green and very dark green) are most preferred, followed by var. virens (light green) and finally var. albus with yellow skin. This indicates an important relationship between the conservation and support of genetic resources of S. edule and the morphological distinctions that mark the preference of the consumer, which strongly impacts domestication or reproductive isolation.
Molecular characterization identified 91 loci with 96.7% polymorphism. The number of effective alleles was 1.18 per locus (capable of being passed on to the next generation). The expected heterozygosity had an average value of 0.127 ± 0.013, indicating moderate levels of genetic diversity compared to other species that used the same markers, such as Zingiber officinale (0.347) [24].
Groups of plants strongly manipulated by humans can reflect different life histories because of agroclimatic variables, reproductive isolation, and domestication processes. In this study, genetically differentiated groups were observed, some more than others, with evidence of subgrouping. Cadena-Iñiguez et al. [7] noted that a transition in the color of the fruit from dark green to light green and even to yellow was the result of adaptive specialization to the environment. Meyer et al. [25] indicated that under the domestication syndrome described by Harlan [26], changes were noted in the size of the fruit, seeds, appearance of the plant, and in secondary metabolites, among other traits of the plants. For instance, var. amarus sylvestris has dark green fruit and a strongly bitter flavor, while the groups nigrum and virens have fruit with a neutral flavor, and albus has slightly sweet fruit [11,27].
Diversity arises by speciation, forming one or more species from an ancestor [27]. Reproductive isolation induces genetic variations and, in turn, causes morphological, physiological, and chemical variations, which cause continuous (clinal) or discontinuous variations [28]. However, in S. edule, the soft seed coat does not allow it to be preserved [29], and there are no archaeological records that prove the existence of an initial edible variety, which suggests that those known today are essentially derived varieties.
In northeastern India, the morphological and genetic characteristics of 74 local varieties of S. edule were evaluated to promote the development of breeding programs. The morphological characteristics were related to the fruit (color, length, width, and weight) and sugar, ascorbic acid, and phenol content. To complement this study, 28 RAPD-type molecular markers and 30 ISSR were added. The RAPD analysis showed a polymorphism of 88.38% and some of the chayote varieties that shared common morphological characteristics (light green and dark green fruit) also shared the same genomic fragments. Referring to the ISSR analysis, a polymorphism of 62.5% was observed. The fruit with light green and dark green tones showed the highest ascorbic acid and phenol content.
Principal component analysis indicated that 77.74% of the total variation was explained by the first three components. The first main component was supported by the morphological characteristics of the fruit (length, width, and color), while the second component was characterized by the chemical characteristics of the fruit (ascorbic acid and total sugar content). Light green chayote varieties showed the highest average rates of polymorphism for ISSR and RAPD markers. The cluster analysis revealed the absence of a relationship between clusters and geographical distances of the chayote varieties. The results obtained, in terms of chemical characteristics, were similar to those reported by Cadena-Iñiguez et al. [11].
In 36 accessions of chayote from India, 18 quantitative and qualitative morphological characteristics of fruits were evaluated. The same study was complemented with 12 DAMD-type molecular markers. Principal component analysis showed that 49.68% of the total variation was explained by the first two components. The main characteristics that contributed to this variation were weight, shape, length, and width of the fruit, as well as length and density of the loin. These characteristics were distinctive of the chayote varietal complexes described by Cadena et al. [11]. Molecular analysis revealed 95% polymorphism, indicating high genetic diversity among chayote accessions. Unlike the study by Verma et al. [12], it was observed that cluster analysis with DAMD-type molecular markers discriminated accessions by geographical distribution (northeast and south India).
Cui et al. [13] sequenced the complete chloroplast genome of S. edule, which was 154,550 bp in size and contained a total of 122 genes (78 for protein coding, eight rRNA genes, and 36 tRNA genes). The guanine-cytokine content was 37.2%. The results of sequencing this genome demonstrated high phylogenetic affinity with other chloroplast genomes of species belonging to the Cucurbitaceae family, mainly Cucumis spp. and Cucurbita spp.
Our study bears similarities to other research on genetic and morphological diversity conducted in India [10,11]. The main components of the analysis indicated that the variation was linked to the morphological characteristics of the fruit (color, shape, size, and weight). On the other hand, high polymorphism was noted in the different types of molecular markers used. The associated analysis between morphological and genetic markers enriches the information regarding these accessions, thus enabling taxonomic and phylogenetic inferences.
The morphological variations observed in S. edule suggest that the plants have conserved the expression of essential characteristics resulting from the initial genotype or from the subsequent combination of genotypes of different varieties [30]. Figures 4 and 5 indicate the genetic closeness between var. amarus sylvestris and the wild type (ancestor). The high level of genetic polymorphism, attributed to adaptive specialization, reproductive isolation, conservation in backyards, intercrossings, constant selection, etc., has not erased the initial characteristics, except for the bitter taste, the loss of which was attributed to cultivation, domestication, and adjustments in the mevalonic acid pathway that modified the cucurbitacin and pigment content, thus facilitating adaptation to new environments [7,11].

Environmental Conditions
The Germplasm National Bank of Sechium edule (BANGESe) is in Veracruz, Mexico (19 • 08 48 N and 97 • 57 00 W). The vegetation type is mountain cloud forest (altitude of 1340 m), with an annual mean temperature of 19-22 • C, relative humidity of 85-90 %, and annual mean precipitation of 2250 mm. The soils are vitric and luvisolic, rich in organic matter, low in calcium, and high in iron, manganese, and zinc nutrients, with moderate fertility, thick texture, and fragments of volcanic glass (pH 4.3-6.5) [11].

Biological Material
The Because previous biochemical, morphological, and anatomical studies showed stability of the distinctive features of S. edule, the number of individuals per accession [2] was reduced and the number of generations evaluated was increased to five. The successive evaluation of the accessions was carried out annually for five generations, harvesting fruit from two chosen plants per accession at physiological maturity, and the plants generated from these fruit were replanted in an open area adjacent to the Genebank. Given the previous results of parsimony and stability, the sample size (n = 133) was maintained in each generational evaluation. To reduce the risk of pollen contamination, successive fruit were selected from the first third of the plant. Multiple plants were generated and identified in order to identify the typical characteristics of the corresponding varietal group. The morphological evaluation was carried out annually for five generations, measuring each year, adhering to the protocol of the Sechium germplasm bank for accumulating evidence of characteristic stability. This was important since the original provenance of each accession differed.

Evaluation of Morphological Characteristics
The sample size for this analysis (n = 133) started from a population of 245 accessions in the germplasm bank. The agroclimatic conditions for all accessions were the same (2.0 ha of collection surface) and their essential characteristics were recorded for for five generations. Table 4 shows the morphometric patterns of the varietal descriptors designed and analyzed separately using qualitative (47) and quantitative (18) measures (UPOV).

DNA Extraction and AFLP Analysis
Young and healthy leaves of the 133 chayote plant accessions were extracted to obtain the DNA using the CTAB (cetyltrimethylammonium bromide) method with some modifications [32]. Throughout the study, the same accessions were used for both morphological and AFLP analyses. Data not reported in this study indicated that the progeny of the evaluated accessions were uniformly conserved and inherited the morphological characteristics in different environments, indicating their stability.
The products from PCR were corroborated on agarose gel (0.8%) for 60 min at 80 V, and later diluted before the pre-amplification reaction was carried out. The products of the amplification were separated by polyacrylamide gel electrophoresis (6%) at 200 V for 3 h, the gels were stained with silver nitrate, and then a 1:10 dilution with formamide was carried out for the PCR-selective products, which were evaluated by capillary electrophoresis using a 3500 XL Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA). For the principal coordinate analysis, the percentage of polymorphism per band and percentage of variation explained were determined using AFLP-SURV 1.0 [33] software, and MEGA v.7 software was used to generate the tree [34]. Using Structure 2.3.4 software [35], a Bayesian analysis was carried out based on the Markov Chain Monte Carlo (MCMC) algorithm in order to assign individuals to populations based on their genotypes, estimating the frequency of population alleles and identifying their structure by explaining the presence of the Hardy-Weinberg or linkage disequilibrium [36,37].
The genetic distances were scored according to the presence (1) or absence (0) of 228 AFLP markers and the Dice coefficients [38]. Construction of the dendogram, generated using the hierarchic clustering method with the unweighted pair group method using arithmetic averages (UPGMA) [39], and principal coordinate analysis were performed using the GenAlEx 6.0 program [40].

Conclusions
Twenty-six varietal descriptors allowed differentiation between the evaluated chayote accessions. The main characteristics that enabled distinction were considered qualitative and quantitative; however, the evaluations under field conditions over five years demonstrated that the features were stable and unalterable from generation to generation. The visual distinction manifested mainly in fruit variables, such as color, shape, size, flavor, and presence/absence of spines. There was a high level of genetic polymorphism between cultivated accessions and their wild ancestor. The polymorphic variations in S. edule suggest that the morphological characteristics have been differentiating from an essentially derived ancestral variant. For this reason, we suggest that the characteristics evaluated in the current study should be adopted as the main varietal descriptors in the technical guide of this phytogenic resource for food and agriculture. These descriptors will update the initial guide (phenetics) for the distinction, registration, and legal protection of varieties, both those of common use and those obtained by breeding. They will also contribute to varietal separation and differentiation for ex situ conservation purposes, basic research, and bioprospective studies in a format suitable for international use.