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The Effect of Basic Microshapes on hNT Astrocytes Cytoplasmic Process Outgrowth

Astrocytes are a non-homogeneous cell type, highly mobile which constantly extend and retract their cytoplasmic processes in what would seem random in direction. In this paper, we investigate how simple geometric microshapes can be used to control the outgrowth of human astrocytes cytoplasmic processes. We investigate the effect of how five regular microshapes: the circle, triangle, square, pentagon and hexagon control astrocyte cytoplasmic process outgrowth. For all the different microshape types, we observe that it is the corners of the shapes that that cause the astrocyte to produce spontaneous outgrowth except for the circle where the outgrowth occurs at a random radial position. This work suggests that the geometry of cell adhesive regions effects the outgrowth of hNT astrocytes.