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Separation and quantitation of bacterial ribonucleoside triphosphates extracted with trifluoroacetic acid, by anion-exchange high-performance liquid chromatography.

Endogenous ribonucleoside triphosphates were determined in Escherichia coli and Pseudomonas aeruginosa by using anion-exchange high-performance liquid chromatography after extraction with trifluoroacetic acid (TFA). Results indicate that in E. coli this extraction method is more sensitive and reliable than trichloroacetic acid (TCA) and formic acid extraction. However, whereas in P. aeruginosa best yields of adenosine 5'-triphosphate and guanosine 5'-triphosphate are obtained following extraction with TFA, the yields of uridine 5'-triphosphate and cytidine 5'-triphosphate can be increased if extraction is performed with TCA.