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THE EFFECTS OF TETRAPHENYLBORON ON THE ISOLATION OF NEURONAL AND GLIAL CELLS FROM GUINEA‐PIG BRAIN

Abstract— The addition of 20 mM‐tetraphenylboron to the tissue‐softening medium of a neuronal and glial cell isolation procedure results in a 2‐fold and 5‐fold increase in the yield of neuronal and glial‐enriched material respectively. The purity of the isolated fractions appears unaltered. While tetraphenylboron strongly inhibits protein synthesis, carbonic anhydrase activity and structural integrity appear unaffected by tetraphenylboron. The potential use of this compound in cell isolations is discussed.