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An Active Compound From Pseudomonas aeruginosa B27

A compound was isolated from the fermented broth of Pseudomonas aeruginosa. The compound was determined as acridine-4-carboxylic acid based on nuclear magnetic resonance, mass spectrometry (MS), and single-crystal x-ray diffraction analysis. Acridine-4-carboxylic acid showed significant antibacterial activities against Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, Bacillus megaterium, and Shinella sp. Moreover, the compound showed certain antitumor activities against HCT116, ST486, HCC1806, and A549 with IC50values of 38.38, 6.59, 20.69, and 82.81 μg/mL, respectively. In the test of nematicidal activity, acridine-4-carboxylic acid showed certain nematicidal activity against Caenorhabditis elegans and Meloidogyne incognita. In addition, the compound also exhibited 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity. Therefore, this compound may be used as a potential antibacterial, antitumor, nematicidal, and antioxidative agent.

Microorganisms are a promising source of natural products with significant contributions to almost every sphere of human, plant, and veterinary life. The significance of natural compounds extracted from microorganisms has been shown in nutrition, agriculture, and healthcare sciences. 1 Numerous studies have shown that microorganisms can produce many novel metabolites, such as steroids, terpenoids, flavonoids, and alkaloids, which have conspicuous antitumor, 2,3 anti-microorganism, 4 anti-inflammatory, 5 antioxidative, 6 and sugar-reducing 7 activities. Microbial metabolites have been used extensively in medicine. 8 Among different microorganisms, actinomycetes are well investigated groups, particularly members of the genus Streptomyces. Bacteria and endophytic fungi are also reported in a large number of antibiotics.
Microorganisms isolated from different environments have become one of the major sources for finding active natural compounds. 9,10 Pseudomonas aeruginosa is a gram-negative, aerobic bacterium that can be widely found in diverse environments, such as soil, water, humans, hospitals, animals, plants, and sewage. Pseudomonas aeruginosa is a typical species, which belongs to the genus Pseudomonas. 11 It is an opportunistic pathogen in humans and plants. In the present study, a B274 strain was isolated from soil, which was identified as P aeruginosa. In this study, the compound acridine-4-carboxylic acid was isolated from the fermented broth of P aeruginosa, and its' antibacterial, antitumor, nematicidal, and antioxidant activities were assayed. At present, there are few reports on the activity of acridine-4-carboxylic acid, mainly focusing on its substituted derivative and activity. [12][13][14] It should be noted that acridine-4-carboxylic acid is isolated from bacteria firstly. Expectably, this compound may be used as a potential antibacterial, antitumor, nematicidal and antioxidative agent. 1

Strain Identification
Based on the 16S rRNA gene sequencing (GenBank MW463450), the strain was highly homologous to P aeruginosa (99%) (http:// blast.ncbi.nlm.nih.gov/Blast.cgi); therefore, strain B274 was considered to be P aeruginosa. Pseudomonas belongs to the Pseudomonadaceae family, which contains 191 validly described species. 15 Pseudomonas aeruginosa is a species of the genus Pseudomonas that is increasingly recognized as an emerging opportunistic pathogen of clinical relevance.

Compound Structure
The compound was isolated from the fermentation broth of P aeruginosa. According to the spectroscopic data and the available literature, 16 the compound was identified as acridine-4-carboxylic acid ( Figure 1). The structure was confirmed by a single-crystal x-ray diffraction analysis ( Figure 1). This compound has been already obtained by chemical synthesis. 12

Antibacterial Activity
The acridine-4-carboxylic acid was assayed in terms of antibacterial activity against S aureus, B cereus, B subtilis, E coli, B megateriu, and Shinella sp. The findings showed that acridine-4-carboxylic acid compound had stronger activity against S aureus, B subtilis, B megateriu, and Shinella sp., whereas it showed fainter effect on B cereus and E coli at concentrations tested (Table 1 and Figure 2).

Antitumor Activity
The antitumor activity of acridine-4-carboxylic acid was investigated by a sulforhodamine B (SRB) assay. The inhibition activity against the tumor cells is presented in Table 2 and Figure 3. The results indicated that the acridine-4-carboxylic acid could effectively inhibit the growth of 3 tumor cells (HCT116, ST486, and HCC1806) in vitro with IC 50 values of 38.38, 6.59, and 20.69 μg/mL, respectively. Whereas it displayed moderate antitumor activity on A549 at concentrations tested.

Discussion
In our study, the acridine-4-carboxylic acid compound was isolated from microorganisms for the first time. This compound showed stronger antibacterial activities to S aureus, B subtilis, B megateriu, Shinella sp., B cereus, and E coli. The result showed that the compound had broad-spectrum and significant antibacterial activity. Moreover, the acridine-4-carboxylic acid could effectively inhibit the growth of 3 tumor cells in vitro and exhibited the nematicidal activity against C elegans and M incognita. Therefore, this compound may be used as a potential antibacterial, antitumor, and nematicidal agent.

Cell Lines and Reagents
The cell lines HCT116, ST486, HCC1806, and A549 were obtained from Kunming institute of zoology, CAS. Also, the RPMI-1640 medium was purchased from Gibco BRL (Life Technologies), and fetal bovine serum was purchased from HyClone Laboratories. Column chromatography (CC) was performed on silica gel G (200-300 mesh, Qingdao Marine Chemical Inc.) and Sephadex LH-20 column (Amersham Pharmacia). Thin layer chromatography was performed on GF254 silica gel (10-40 μm; Qingdao Marine Chemical Inc.). All solvents were distilled before use.

Isolation and Identification of Strain
The strain B274 was isolated from a soil sample of Kunming, China. The strain was deposited in the Southwest Natural Germplasm Center for Microbial Resources (China). The bacterium was identified based on its 16S rRNA sequence using universal bacterial primers, including 8F (5'-GAGTTTGATCCTGGC TCAG-3 ′ ) and 1492R (5'-GTTACCTTGTTACGACTT-3 ′ ). 17 To identify the isolates, PCR amplicons were sequenced by BGI (China), and the resulting sequences were analyzed with the BLAST algorithm of the Ribosomal Database Project II (RDP II). 18 The 16S rRNA gene sequences have been deposited in the NCBI nucleotide sequence database.

Extraction and Isolation of Compound
The strain B274 was grown in LB medium inside flasks (200 mL per 500 mL of Erlenmeyer flask) on a shaker incubator. After fermentation for 3 days at 37°C at 180 rpm, the fermentation broth of 3 L B274 was centrifuged. The filtrate was then condensed to 1 L by rotary evaporation and extracted with an equal volume of butanol to obtain a crude extract (3.4 g). The crude extract was purified on a Sephadex LH-20 column (600 g), eluted with methanol to yield fractions A1-A9. The fraction A5 (543 mg) was subjected to silica gel G (15 g) CC eluting with EtOAc/methanol by increasing polarity from 100:1 to 20:1 to yield A5-1-A5-4 fractions. Fraction A5-2 (274 mg) was purified on the Sephadex LH-20 column (60 g), eluted in methanol to yield fractions A5-2-1-A5-2-3. The fraction A5-2-2 (78 mg) was further purified on the Sephadex LH-20 column (20 g) and eluted with acetone to obtain compound 1 (14 mg).

Determination of Antibacterial Activity
The antibacterial activity was determined using the disc diffusion method, according to Motta and Brandelli's method 19 with minor modifications. The strains S aureus, B cereus, B megaterium, B subtilis, E coli, and Shinella sp. were used in our study. They were grown in tubes containing 5 mL of LB medium, and then incubated for 12 h at 30°C at 180 rpm. Then, 100 μL of bacterial broth was used to coat the LB medium plates. The compound acridine-4-carboxylic acid (200, 100, and 50 μg/disc) was added on a filter paper (diameter, 0.6 cm), and then, each filter paper was attached to the plate. The same amount of chloroform was used as the control. The size of the inhibition zone was measured after culturing for 24 h at 30°C.

Antioxidant Activity Assay
The antioxidant activities of acridine-4-carboxylic acid were measured in the DPPH radical scavenging assay with a slight modification of the reported method. 21 Briefly, 100 μL 0.2 mM DPPH prepared in MeOH was added to 96-well dishes with compound at different concentrations. The samples were incubated for 30 min in the dark at room temperatures, and the decrease of absorbance at 515 nm was measured against MeOH using a Microplate reader. The DPPH solution was freshly prepared and used within 12 h. All determinations were performed in more than triplicate. The radical scavenging activity of the tested samples was calculated as "inhibition percentage" according to the equation: Inhibition percentage(%) = [(As − Ab)/(As − Aref )] × 100% Note: As represents the OD value of the test sample and the DPPH mixture solution, Ab represents the OD value of the

Nematicidal Activity
The nematicidal activity assay of the target compound against Meloidogyne incognita and Caenorhabditis elegans refers to the method in the literature. 22,23 Caenorhabditis elegans was synchronized and cultured at 20°C. Meloidogyne incognita used diluted sodium hypochlorite (NaOCl) solution to extract the eggs of Meloidogyne incognita from the rhizomes of infected tomatoes (Solanum lycopersicum). The strainer was placed in a petri dish containing water and incubated at 25°C. The second instar larva of Meloidogyne incognita (J2) and the fourth instar larva of Caenorhabditis elegans (L4) were collected every day and used directly for biological activity testing. The target compound was dissolved in a mixture of sodium hydroxide: absolute ethanol: ddH 2 O = 2:9:9 (pH value was neutral), and diluted with water and M9 solution to prepare the test solution. The test solution was added to a 24-well tissue culture plate. The concentration of second-instar nematodes per well was about 100 second-instar nematodes per milliliter of water. Then covered the 24-well tissue culture plate cover and proceed at (25 ± 1)°C cultivation, each treatment was repeated 3 times, and the mortality of nematodes was observed with a stereo microscope after 24 h. If the nematode body was still (for example, the body was straight), even if the nematode remains still after being transferred to clean water for 12 h, it was considered that the nematode is dead.