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Multiplexed spatial-spectral fluorescent holographic imaging (Conference Presentation)

The biggest challenge on the path towards high-dimensional imaging is obtaining spatial and spectral information of a volumetric sample in real-time. Advances in this field are of vital importance for biology, material sciences, and medical applications. For example, cancer is one of the leading causes of death in the world, thus gaining a mechanistic understanding of cancer cell processes will significantly impact therapies targeting cancer metastasis pathways. Revealing the entire process with its surrounding environment will require real-time imaging systems that will open the way for better understanding of cancer onset and tissue morphology. This talk will introduce real-time volume holographic imaging systems, which are based on multiplexed volume holographic (MVH) gratings acting as spatial-spectral filters used in conventional optical imaging systems. This allows the acquisition of spatial images with spectral selectivity but without scanning in both transverse and longitudinal directions. In addition, light sheet fluorescent microscopy (LSFM) has recently been utilized to recover 3D images of biological samples. Through pupil engineering, a compact LSFM imaging system will be presented to provide fine optical sectioning capability in more compact fashion. Experimental results of in vivo images of biological samples (e.g. C. elegans) will be demonstrated via our LSFM.