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Nucleic Acids Research during development : mUla RNAs

I demonstrate that the U. snRNAs of human cells are heterogeneous in sequence. Polyacrylamide gel and RNase Ti fingerprint analyses of Ul RNAs isolated from a variety of human cultured cells, including HeLa, 293, K562 and NT2/D1, show that minor variants of the human U] RNA (hUla) comprise between 5% and 15% of the total Ul RNAs in these established cell lines. The patterns of variants are cell line specific, suggesting that expression of these minor species of hUla RNAs reflect polymorphisms of the hUla true genes rather than existence of an additional class of human embryonic Ul genes. Also, the hUla variants described here are not the products of previously identified human U] Class I pseudogenes.