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SANDWICH IMMUNOASSAY FOR MONOCLONAL ANTIBODY USING PROTEIN G IMMUNOAFFINITY CAPILLARY CHROMATOGRAPHY AND DIODE LASER INDUCED FLUORESCENCE DETECTION

A sandwich immunoassay for monoclonal antibody using protein G immunoaffinity capillary chromatography and LIF detection was described. Monoclonal anti-bovine serum albumin (BSA) was used as a model to test the performance of the system. Cy5 was used as the fluorescent tag and the 635 nm line of a diode laser was used as the excited source. Capillaries with inner diameter of 150 μm were packed with recombinant protein G bound perfusive support and used to capture the immunocomplexes, which were subsequently desorbed by an acidic buffer and then detected using LIF. The results demonstrated the feasibility to perform sandwich immunoassay using this system.