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The role of carbamino compounds in histological staining

The effects of high CO2 concentration on tissue staining with buffered eosin, methylene blue and azure A were investigated to determine if carbamino compound formation could alter dye binding. Only in the case of eosin staining were the differences in results with high and low CO2 concentrations compatible with the formation of carbamino compounds. The differences observed with basic dyes were statistically significant but could not be accounted for by carbamino compound formation.