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Haemolysis and Intravascular Coagulation due to Incompatible Red Cell Transfusion in Isoimmunized Monkeys

Summary. Ten randomly selected cynomolgus monkeys were each given an intramuscular injection of a mixture of isologous red cells suspended in complete Freund's adjuvant. After 6 weeks a second injection of red cells in incomplete Freund's was given and thereafter intravenous booster injections of red cells in isotonic saline were used. Antibody titres were determined with serial crossmatches at 37°C, 4°C, and with rabbit antimonkey serum. All animals developed antibody to at least one and several against all four immunizing red cells. Haemolysins were seen frequently.