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Structure and chromosomal localization of the human 2‘,3’‐cyclic nucleotide 3‘‐phosphodiesterase gene

Human brain cDNA clones for the myelin associated enzyme 2′ 3′ ‐cyclic nucleotide 3′ ‐phosphodiesterase (CNPase) have been isolated and sequenced. The only 5′ untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI mRNA. Human CNPase cDNAs were used to isolate genomic clones containing the human CNPase gene which is 9 kb long. Four exons were identified, separated by three introns, and the sequence of each exon and intron/exon boundary has been established.