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[Effect of trehalose on survival rate for  fat cells after cryopreservation].

OBJECTIVE
To explore effects of trehalose as a cryopreserve agent on survival rate of fatty tissue after cryopreservation.  Methods: The liposuction was used on the abdomen of adult female. After centrifugation and purification, adipose was randomized into the following three groups, the trehalose group, the fetal bovine serum (FBS)+10%DMSO group and the physiological saline group. The specimens were cryopreserved at -196 ℃ for 3 months and then the HE staining, glucose transfer method and CK method were used to detect the cell survival rate in each group.  Results: The activity of adipose in the trehalose group and FBS+10%DMSO group adipose was higher than that in the physiological saline group (P<0.05); while there was no significant difference between the trehalose group and FBS+10%DMSO group (P>0.05).  Conclusion: As cryoprotectant, trehalose could keep fat cell viability, and adipose tissue can be used for clinical transplantation after 3 months' freezing.