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Podocalyxin Is a Glycoprotein Ligand of the Human Pluripotent Stem Cell‐Specific Probe rBC2LCN

In comprehensive glycome analysis with a high‐density lectin microarray, we have previously shown that the recombinant N‐terminal domain of the lectin BC2L‐C from Burkholderia cenocepacia (rBC2LCN) binds exclusively to undifferentiated human induced pluripotent stem (iPS) cells and embryonic stem (ES) cells but not to differentiated somatic cells. Here we demonstrate that podocalyxin, a heavily glycosylated type 1 transmembrane protein, is a glycoprotein ligand of rBC2LCN on human iPS cells and ES cells. When analyzed by DNA microarray, podocalyxin was found to be highly expressed in both iPS cells and ES cells. Western and lectin blotting revealed that rBC2LCN binds to podocalyxin with a high molecular weight of more than 240 kDa in undifferentiated iPS cells of six different origins and four ES cell lines, but no binding was observed in either differentiated mouse feeder cells or somatic cells. The specific binding of rBC2LCN to podocalyxin prepared from a large set of iPS cells (138 types) and ES cells (15 types) was also confirmed using a high‐throughput antibody‐overlay lectin microarray. Alkaline digestion greatly reduced the binding of rBC2LCN to podocalyxin, indicating that the major glycan ligands of rBC2LCN are presented on O‐glycans. Furthermore, rBC2LCN was found to exhibit significant affinity to a branched O‐glycan comprising an H type 3 structure (Ka, 2.5 × 104 M−1) prepared from human 201B7 iPS cells, indicating that H type 3 is a most probable potential pluripotency marker. We conclude that podocalyxin is a glycoprotein ligand of rBC2LCN on human iPS cells and ES cells.


INTRODUCTION
Human induced pluripotent stem (iPS) cells and embryonic stem (ES) cells with characteristics of self-renewal and pluripotency are attractive sources of cells for cell-replacement therapies. Before pluripotent stem cells can be used clinically, however, an important safety concern to be addressed is that residual undifferentiated cells could form tumors in patients. The use of cell surface pluripotency markers would serve as a standard and indeed a powerful technique to detect, and ideally remove, such teratomaforming undifferentiated cells. Monoclonal antibodies that include anti-SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81 have been used as probes for this purpose [1][2][3]. Among them, SSEA-3 and SSEA-4 are carried by globo-series glycolipids, consisting of Gal␤1-3GalNAc␤1-3Gal␣1-4Gal␤1-4Glc-Cer and its ␣2-3-sialylated form (Sia␣2-3Gal␤1-3GalNAc␤1-3Gal␣1-4Gal␤1-4Glc-Cer), respectively. On the other hand, TRA-1-60 and TRA-1-81 markers have been reported to be expressed on podocalyxin, a heavily glyco-sylated membrane protein [4]. Their carbohydrate epitopes were first reported to be keratan sulfate but were recently identified by glycan microarray analysis to be type 1 N-acetyllactosamine (Gal␤1-3GlcNAc) [5]. More recently, anti-SSEA-5 was proposed as a novel antibody probe to detect as well as remove human iPS cells and ES cells through its specificity to H type 1 glycan (Fuc␣1-2Gal␤1-3GlcNAc), although its carrier protein has not been identified [6]. It should be noted that all of these cell surface pluripotency markers are "carbohydrate antigens" that have been known to reflect the degree of cell differentiation/undifferentiation [3].
To understand the cellular glycome of human iPS cells and ES cells in a comprehensive way, we previously performed glycome analysis of a large set of human iPS cells (114 cell types) and ES cells (9 cell types) using an advanced high-density lectin microarray [7]. In that study, the expression of ␣2-6Sia, ␣1-2Fuc, and type 1 LacNAc was strongly indicated to be increased upon induction of pluripotency [7], and consistent with this, responsible glycosyltransferase genes, that is, ST6Gal1/2, FUT1/2, and B3GalT5, involved in the synthesis of these glycan epitopes showed significant increases in expression levels [7]. Furthermore, we found that a lectin designated rBC2LCN (recombinant N-terminal domain of BC2L-C), which was originally derived from Burkholderia cenocepacia, bound all of the undifferentiated cells tested, but not at all to any of the differentiated cells [7,8]. Notably, this lectin was shown to have a strong preference to bind the defined epitope structure Fuc␣1-2Gal␤1-3GlcNAc/GalNAc (H type 1/3/4), which comprises two of the above characteristics of pluripotency, that is, ␣1-2Fuc and type 1 LacNAc [7]. rBC2LCN is a small protein with a molecular weight of 15 kDa having a compact jellyroll architecture composed of 11 ␤ strands and a short ␣ helix, similar to the structure of tumor necrosis factor-like proteins [8]. rBC2LCN can be expressed at high levels in a soluble form in the cytoplasm of Escherichia coli (up to 80 mg/l) and can be purified to homogeneity in a one-step sugar-immobilized affinity chromatography approach [7]. rBC2LCN is highly specific to the defined glycan epitope Fuc␣1-2Gal␤1-3GlcNAc/GalNAc, which is contained in glycans such as H type 1 (Fuc␣1-2Gal␤1-3GlcNAc), H type 3 (Fuc␣1-2Gal␤1-3GalNAc), Le b (Fuc␣1-2Gal␤1-3(Fuc␣1-4)GlcNAc), and Globo H (Fuc␣1-2Gal␤1-3GalNAc␤1-3Gal␣1-4Gal␤1-4Glc) [8]. Therefore, rBC2LCN, unlike antibody, could serve as a novel type of detection reagent of pluripotent stem cells [9], particularly given that it is cost-effective and easy to produce in large amounts.
Here we demonstrate that podocalyxin, previously known as a Tra-1-60/81 carrier protein, functions as the glycoprotein ligand of the human pluripotent stem cell-specific probe rBC2CLN on undifferentiated iPS cells and ES cells [10]. Furthermore, we report evidence that H type 3 is a most probable pluripotent glycan marker responsible for this lectin probe.

Cell
Human endometrium (UtE1104), amnion (AM936EP), and placental artery endothelium (PAE551) were collected by scraping tissues from surgical specimens as a therapy under signed informed consent, with ethical approval of the Institutional Review Board of the National Institute for Child Health and Development of Japan [11]. All experiments handling human cells and tissues were performed in line with the tenets of the Declaration of Helsinki. Endometrium, amnion, and placental artery endothelium cell lines were independently established. However, their actual origins remain to be clarified. Human iPS cells (MRC5-iPS, AM-iPS, UtE-iPS, and PAE-iPS) were then generated via procedures described by Yamanaka and colleagues [12,13] and others [7, 14 -16]. iPS201B7 and iPS253G1 human iPS cells were cultured in DMEM/Ham's F-12 medium (Invitrogen, Carlsbad, CA, http://www.invitrogen.com) supplemented with 20% Knockout serum replacement (KSR) (Invitrogen), 0.1 mM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO, http://www.sigmaaldrich. com), minimal essential medium (MEM) nonessential amino acids (Invitrogen), and 10 ng/ml recombinant human basic fibroblast growth factor (Wako) on mitomycin C-treated mouse embryo fibroblast feeder cells. Human iPS cells (TIG/MKOS nos. 19, 56, and 106) were generated through reprogramming by Sendai virus infection-mediated expression of OCT4, SOX2, KLF4, and c-MYC as previously described [17]. The production of iPS cells was confirmed by immunofluorescence staining using established stem cell markers (Nanog, Oct3/4, SSEA4, and TRA-1-60) and analysis of the capacity for teratoma formation (supplemental online Fig. 1). Human ES cells (KhES1, KhES2, KhES3, and HUES1) were obtained and maintained as previously described [18,19]. A list of the cell lines used in this study and their associated characteristics is given in supplemental online Table 1.
(nos. 16 -153), and ES cells (nos. 154 -168), treated with Arthrobacter ureafaciens sialidase (Roche, Indianapolis, IN, http://www. roche.com; catalog no. 10269611001, 1 l) in PBST at 37°C overnight and incubated with high-density lectin microarray at 20°C overnight (supplemental online Fig. 3). After washing with probing buffer (25 mM Tris-HCl, pH 7.5, 140 mM NaCl [TBS], 2.7 mM KCl, 1 mM CaCl 2 , 1 mM MnCl 2 , and 1% Triton X-100), the array was blocked with 60 l of rabbit normal IgG (50 g/ml) at 20°C for 1 hour. After washing again with probing buffer, the array was reacted with biotinylated goat anti-podocalyxin pAb (R&D; catalog no. AF1658) for 1 hour at 20°C. After a further wash with probing buffer, bound anti-podocalyxin pAb was detected with 1 g/ml of Cy3-labeled streptavidin at 20°C for 30 minutes. After washing with probing buffer, fluorescence images were acquired using an evanescent field-activated fluorescence scanner (Glyco-Station Reader 1200; GP BioSciences, Sapporo, Japan, http:// www.gpbio.jp/english/index.html). The fluorescence signal of each spot was quantified using Array Pro Analyzer ver.4.5 (Media Cybernetics, Bethesda, MD, http://www.mediacy.com), and the background value was subtracted. The lectin signals of triplicate spots were averaged and normalized to the mean value of 96 lectins immobilized on the array to adjust the data from each microarray to account for possible systematic variation [20,25].

Gene Expression Analysis
Total RNA was extracted from each cell sample using ISOGEN (Nippon Gene, Tokyo, Japan, http://www.nippongene.com). Global gene expression patterns were monitored using whole human genome microarray chips (G4112F; Agilent Technologies, Palo Alto, CA, http://www.agilent.com) with one-color (Cyanine 3) dye. Hybridization was determined with a G2505C microarray scanner system (Agilent). The data were analyzed using Gene-Spring GX12.0 software (Agilent). Each chip was normalized to the 75th percentile of measurement taken from the chip.

Podocalyxin Is a Candidate Ligand of rBC2LCN as Revealed by DNA Microarray
Using the high-density lectin microarray, we have previously shown that a probe lectin, rBC2LCN, exhibits rigorous specificity to undifferentiated human iPS cells and ES cells, but not at all to differentiated somatic cells [7]. Therefore, identification of glycoprotein ligands of rBC2LCN is critical to determine the molecular mechanism of undifferentiation from both developmental and glycobiological viewpoints. As a practical approach, we focused our attention to a candidate glycoprotein, podocalyxin, with respect to the following criteria: (a) highly expressed in human iPS cells and ES cells; (b) correlated with Nanog expression (pluripotency marker); (c) localized at the cell surface; (d) heavily glycosylated; and (e) antibodies used for immunoprecipitation are commercially available. In gene expression analysis of human iPS cells, ES cells, and somatic cells using DNA microarray, the podocalyxin gene was found to be highly expressed in all of the human iPS cells and ES cells (criteria a and b) ( Fig. 1). High expression was also detected in one of the somatic cells derived from placental artery endothelial (PAE) and low but significant expression in other three somatic cells of MRC5, amniotic mesodermal (AM), and uterine endometrium (UtE) at the transcription level. Human podocalyxin is a type 1 membrane protein consisting of 528 amino acids, with criterion c thus satisfied given that the protein is localized at the cell surface [28]. The extracellular domain of podocalyxin has a mucin domain with a high serine/ threonine content for putative O-glycosylation modifications. Podocalyxin also has five potential N-linked glycosylation sites and three putative glycosaminoglycan sites. Indeed, the apparent molecular weight has been reported to be ϳ200 kDa in embryonic carcinoma cells [29], despite the calculated molecular weight of 55 kDa, demonstrating that human podocalyxin is heavily glycosylated (criterion d). Various types of polyclonal and monoclonal antibodies raised against the extracellular and the intracellular domains of human podocalyxin are commercially available (criterion e).

High Molecular Weight Proteins of >240 kDa Are the Glycoprotein Ligands of rBC2LCN in Human iPS Cells and ES Cells
To search glycoprotein ligands of rBC2LCN in human iPS cells and ES cells, hydrophilic fractions of human iPS cells and ES cells in addition to mouse feeder cells and somatic cells were first separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted with HRP-conjugated rBC2LCN. , supporting the previous report that rBC2LCN is highly specific to undifferentiated cells [7]. In iPS cells and ES cells, strong and diffuse protein bands were detected at a high molecular weight of Ͼ240 kDa, whereas weaker bands were also detected from 70 to 140 kDa, indicating that other glycoprotein ligands of rBC2LCN are also present in human iPS and ES cells.

Podocalyxin Is a Glycoprotein Ligand of rBC2LCN
The molecular nature of the rBC2LCN-positive bands such as a high molecular weight and high expression in human iPS cells and ES cells strongly suggested that podocalyxin is a glycoprotein ligand of rBC2LCN. Streptavidin-coated magnetic beads immobilized with biotinylated goat anti-podocalyxin pAb were incubated overnight at 4°C with hydrophilic fractions of iPS cells. After extensive washing of the beads, bound fractions were eluted with TBS containing 0.2% SDS at 95°C for 10 minutes. The elution fractions were then separated by SDS-PAGE and blotted with goat anti-podocalyxin pAb followed by HRP-labeled donkey anti-goat IgG. As shown in Figure 3 (bottom panel), podocalyxin could be immunologically detected as a diffuse high molecular weight band at Ͼ240 kDa in the immunoprecipitates from human iPS cells and ES cells, but not those from mouse feeder cells and somatic cells. Because the calculated molecular weight of podocalyxin is 55 kDa, it is evident that podocalyxin, expressed in human iPS cells and ES cells, is heavily glycosylated. Indeed, rBC2LCN exhibited strong binding to the immunoprecipitated podocalyxin as a diffuse protein band at Ͼ240 kDa in human iPS cells and ES cells (Fig. 3, top panel). Stronger staining was observed with rBC2LCN relative to anti-podocalyxin pAb. The rBC2LCN reactivity varied among the cell types of iPS cells and ES cells. Stronger signals were observed for UtE-iPS A04 (P27) (71) and PAE-iPS01 (P26) (95), whereas weaker signals were detected for TIG/MKOS19 (P37) (141), iPS201B7 (P45) (123), and iPS253G1 (P32) (128). Although the podocalyxin gene was detected in PAE551Ar, the corresponding podocalyxin protein was not detected in the anti-podocalyxin immunoprecipitates (Fig. 3), indicating that the podocalyxin expression is restricted to undifferentiated cells at the translation level among the cell types used in this study (supplemental online Table 1). It should be noted that podocalyxin could be precipitated from hydrophilic but not hy-drophobic fractions (data not shown), even though podocalyxin is basically a transmembrane protein. This can be attributed, at least in part, to the highly hydrophilic nature of podocalyxin, which is heavily glycosylated. Altogether, these results clearly demonstrate that podocalyxin is a glycoprotein ligand of rBC2LCN, although the possibility that other glycoprotein ligands with Ͼ240 kDa are included remains.

Podocalyxin Is a Glycoprotein Ligand of rBC2LCN Common to Human iPS Cells and ES Cells Revealed by Antibody-Overlay Lectin Microarray
To examine whether rBC2LCN binds to human podocalyxin derived from human pluripotent stem cells of more than 100 samples in a high-throughput manner, we applied the recently developed high-throughput technology called antibody-overlay lectin microarray, which allows interaction analysis between immobilized lectins and a nanogram order of target samples [10]. A schematic representation of the technique is shown in supplemental online Figure 3. The human podocalyxin immunoprecipitated from human iPS cells and ES cells was reacted with rBC2LCN immobilized on a glass slide [7,23]. After blocking of nonspecific binding sites with normal rabbit IgG, bound podocalyxin was selectively visualized with biotinylated anti-podocalyxin pAb followed by Cy3-labeled streptavidin, according to the established protocol [10]. The binding signals were detected and quantified using an evanescent-field fluorescence-assisted scanner as described [23]. To further confirm this, the immunoprecipitated podocalyxin was pretreated with A. ureafaciens sialidase before application to the lectin microarray, because this treatment was found to enhance the interaction between podocalyxin and the immobilized rBC2LCN. This could be explained in part by reduced electric repulsion caused by the strong negative  [30]. Using the advanced high-throughput technology, a series of cell samples including 138 types of human iPS cells prepared from six different origins with various passages (16 -153 in supplemental online Table 1) and 15 types of human ES cells (154 -168) were analyzed [20]. For reference (negative control), mouse feeder cells (MEFs) (1) and differentiated somatic cells of the iPS origin (2-15) were also examined. Figure 4A shows the results of rBC2LCN binding for representative samples, that is, MEFs (1), fibroblasts (166), iPS cells (115), and ES cells (154), whereas Figure 4B provides a bar graph representation of the total analysis. It was verified unambiguously that all of the iPS cells and ES cells examined bound to rBC2LCN, although the binding degrees were significantly varied. On the other hand, no detectable signal was observed for rBC2LCN binding to feeder (1) or somatic cells (2-15) as expected, since podocalyxin was not immunoprecipitated from these cell samples (Fig. 3). These results confirm the fact that podocalyxin is a universal glycoprotein ligand of rBC2LCN in human undifferentiated pluripotent cells.

Glycan Ligands on Podocalyxin Recognized by rBC2LCN Are O-Glycans
Having demonstrated that podocalyxin is a substantial glycoprotein ligand of rBC2LCN both in iPS cells and ES cells, we next  analyzed the glycan structures on podocalyxin and the classes of glycans to which rBC2LCN binds. For this purpose, we first performed antibody-overlay lectin microarray assisted with enzyme treatments (supplemental online Fig. 4). Since podocalyxin has been reported to contain N-and O-glycans, and keratan sulfate glycosaminoglycans [4,28,[31][32][33], the protein was digested with PNGase F, an amidase that cleaves N-glycans between the innermost GlcNAc and asparagine residues of N-linked glycoproteins, and with keratanase II from Bacillus sp. Ks36, an endo-␤-Nacetylglycosaminidase that hydrolyzes keratan sulfate between the 4GlcNAc␤1-3Gal␤1 structure [34]. Before the enzyme treatments, podocalyxin was heat-denatured and treated with A. ureafaciens sialidase to increase the enzyme susceptibility. Podocalyxin immunoprecipitated from TIG/MKOS19 (P50) chosen as a representative iPS cell line was treated with either sialidase, sialidase and PNGase F, or sialidase and keratanase II and incubated with high-density lectin microarray. Bound podocalyxin was analyzed by the antibody-overlay method as described above. As shown in supplemental online Figure 4, the signals for SNA (Sambucus nigra agglutinin), an ␣2-6Sia-binding lectin, were significantly decreased after the sialidase treatment, demonstrating that podocalyxin is ␣2-6sialylated. The residual signals are attributable to either the incomplete sialidase digestion or the binding to asialo N-glycans, because SNA also shows weak but significant binding to LacNAc (Gal␤1-4GlcNAc) [35]. With the sialidase and PNGase F double treatments, the binding of a high-mannose-type N-linked glycan-binding lectin (recombinant griffithsin [rGRFT]) was also decreased. This indicates that podocalyxin carries high-mannose type N-linked glycans [32]. The binding of Psathyrella velutina lectin (PVL) with specificity to nonsubstituted ␤GlcNAc and 6-O-substituted ␤GlcNAc, including 6S-GlcNAc at the nonreducing end [36,37] was decreased upon double treatments with sialidase and keratanase II. This observation supports a previous report that podocalyxin contains keratan sulfate, which is susceptible to keratanase II treatment [4]. The results also suggest that keratan sulfate is largely attributed to O-glycans, since the PVL signal was not susceptible to PNGase F treatment. In contrast, the signals of an O-glycan binder (Amaranthus caudatus lectin [ACA]) were increased after sialidase treatment and further enhanced with sialidase and keratanase II double treatments. Increased signals can be explained by the increased accessibility of this O-glycan binding lectin caused by the decreased charge repulsion and/or steric hindrance caused by heavy sialylation and sulfation of podocalyxin. Furthermore, significant signals of the ␣1-2fucose-binding lectin MCA could be observed, which were also increased upon enzyme treatments. Similarly, the signals of rBC2LCN were increased after sialidase treatment and further enhanced with the sialidase and keratanase II double treatment, leading to the hypothesis that the major carbohydrate antigens of rBC2LCN are O-glycans.
To prove this hypothesis, we further performed a lectin blotting experiment. The podocalyxin immunoprecipitated from iPS cells (iPS201B7 [P45], 123) and ES cells (KhES-1 [P26], 156) was treated with or without PNGase F, run on SDS-PAGE, and blotted onto PVDF membrane. The membrane was then treated with or without 0.05 M NaOH at 40°C for 16 h to remove O-glycans by ␤-elimination [21,22] and analyzed by lectin blotting using HRPconjugated rBC2LCN. As shown in Figure 5, most rBC2LCN signals were still left upon PNGase F treatment, whereas the alkaline hydrolysis greatly reduced the rBC2LCN binding, demonstrating that the carbohydrate antigens of rBC2LCN are expressed on O-glycans of podocalyxin prepared from human iPS cells and ES cells. Since the alkaline treatment gave essentially no effect on the rBC2LCN binding to a positive control neoglycoprotein (Fuc␣1-2Gal␤1-3GlcNAc␤1-3Gal␤1-4Glc-BSA), the reduced staining is not due to the loss of the blotted proteins.

rBC2LCN Recognizes an O-Glycan Containing an H Type 3 Isolated From iPS Cells
Recently, we performed quantitative glycome analysis targeting both N-and O-glycans of undifferentiated iPS cells (201B7) and differentiated human dermal fibroblasts by a glycosidase-assisted high-performance liquid chromatography (HPLC) method combined with mass spectrometry [38]. Among the 37 types of N-glycans and 10 types of O-glycans identified from iPS cells, one O-glycan with an m/z value of 973.4 [MϩH] ϩ containing an H type 3 (Fuc␣1-2Gal␤1-3GalNAc) was isolated, of which structure was identified to be Fuc␣1-2Gal␤1-3(Gal␤1-3GlcNAc␤1-6)GalNAc-PA by HPLC mapping assisted with matrix-assisted laser desorption ionization time-of-flight mass spectrometry and exoglycosidase digestion analyses [38], where "PA" represents a reducing terminal pyridylamino group. On the other hand, no glycan containing H type 1 was detected. Thus, we determined the association constant between rBC2LCN and this O-glycan carrying an H type 3 structure (hereafter designated glycan a) using a quantitative frontal affinity chromatography (FAC) technique [26] (Fig. 6). For comparison, its isomeric O-glycan containing an H type 2 structure (Fuc␣1-2Gal␤1-4GlcNAc␤1-6(Gal␤1-3)GalNAc-PA, designated glycan b), was analyzed, which was also prepared from 201B7 iPS cells. As a result, rBC2LCN was found to bind glycan a containing the H type 3 structure with a K a of 2.5 ϫ 10 4 M Ϫ1 , whereas no detectable binding was observed for the related glycan b containing the H type 2 structure (K a Ͻ 6.7 ϫ 10 3 M Ϫ1 ). Consistent with a recent study using glycan microarray [7], the present FAC analysis using 126 standard PA glycans confirmed that rBC2LCN bound to H type 1 (Fuc␣1-2Gal␤1-3GlcNAc␤1-3Gal␤1-4Glc-PA, K a ϭ 2.8 ϫ 10 4 M Ϫ1 ) and Le b (Fuc␣1-2Gal␤1-3(Fuc␣1-4)GlcNAc␤1-3Gal␤1-4Glc-PA, K a ϭ 2.0 ϫ 10 5 M Ϫ1 ), but not to other glycans without the defined epitope structure Fuc␣1-2Gal␤1-3GlcNAc(GalNAc). They include high-mannose-type, agalactosylated, galactosylated, sialylated N-glycans, and glycolipid-type glycans, demonstrating that rBC2LCN is highly specific to this glycan epitope (data not shown). Combined with the facts that podocalyxin is heavily Oglycosylated on its mucin domain and that podocalyxin binding to rBC2LCN was decreased by an alkaline treatment, it is most likely that the carbohydrate antigens on podocalyxin recognized by rBC2LCN are H type 3-containing O-glycans, such as glycan a isolated from iPS201B7 cells.

DISCUSSION
Podocalyxin is a type I transmembrane sialoglycoprotein of the CD34 family, which was originally cloned from the human kidney as a component of the podocyte cell glycocalyx [28]. Later, podocalyxin was reported to act as a pluripotent stem cell marker, which is highly expressed on human ES cells [39 -42]. Subsequently, podocalyxin was identified as a carrier molecule of the well-known pluripotency stem cell markers TRA-1-60 and TRA-1-81 [4]. In this study, we have clearly demonstrated that podocalyxin is a glycoprotein ligand of rBC2LCN, a novel probe for human pluripotent cells [7]. This indicates a possibility that podocalyxin functions as a ligand of both probes (TRA-1-60/81 and rBC2LCN) specific to human pluripotent stem cells.
In lectin-blotting analysis, rBC2LCN bound exclusively to undifferentiated human iPS cells and ES cells, but not at all to differentiated somatic cells, supporting the previous finding that rBC2LCN is highly specific to pluripotent stem cells [7]. Surprisingly, podocalyxin was found to be the major glycoprotein ligand of rBC2LCN in human iPS cells and ES cells. However, this could be explained by the unique molecular nature of podocalyxin,  . rBC2LCN binds to an O-glycan containing H type 3 isolated from induced pluripotent stem (iPS) cells. rBC2LCN was immobilized onto NHS-activated Sepharose 4FF (GE Healthcare) and packed into a miniature column and connected to a high-performance liquid chromatograph. PA glycans (glycans a and b, solid lines) prepared from human iPS cells (201B7) were injected into the column. The elution profile was then detected by fluorescence (excitation, 285 nm; emission, 350 nm). The elution front of PA glycan relative to that of negative control PA glycan (Man␣1-6(Man␣1-3)Man␤1-4GlcNAc␤1-4GlcNAc-PA, dotted line), referred to as V-V 0 , was then determined. Woolf-Hofstee plots were prepared using the V-V 0 value. The intercept and slope of the fitted line represent B t (nmol) and ϪK d (M), respectively. Analysis of concentration dependence was performed using globo H (Fuc␣1-2Gal␤1-3GalNAc␤1-3Gal␣1-4Gal␤1-4Glc)-p-nitrophenol. Abbreviation: PA, pyridylaminated. that is, highly expressed in pluripotent stem cells and heavily glycosylated, whereas altered glycosylation machinery upon induction of pluripotency should affect various glycoproteins rather than a specific one. In fact, rBC2LCN-positive bands were detected from 70 to 140 kDa (Fig. 2), but their contribution is rather small compared with podocalyxin. Notably, the podocalyxin protein was detectable at a similar degree in iPS cells and ES cells, whereas the rBC2LCN reactivity was varied among the cell types of iPS cells and ES cells. In this context, it is interesting to speculate that the rBC2LCN reactivity might correlate with the degree of differentiation of pluripotent stem cells.
Alkaline digestion, but not PNGase F treatment, reduced the binding of rBC2LCN to podocalyxin, indicating that the carbohydrate antigens on podocalyxin are presented partly on O-glycans. Previously, we have performed quantitative glycome analysis targeting both N-and O-glycans of undifferentiated iPS cells (201B7) and differentiated human dermal fibroblasts by a glycosidase-assisted HPLC method combined with mass spectrometry [38]. Among 47 types of N-and O-glycans identified from iPS cells, a potential carbohydrate antigen of rBC2LCN was identified, with an O-glycan-carrying H type 3 epitope structure (Fuc␣1-2Gal␤1-3(Gal␤1-3GlcNAc␤1-6)GalNAc-PA), whereas H type 1 could not be detected on either N-and O-glycans. Indeed, rBC2LCN bound to this O-glycan with a K a of 2.5 ϫ 10 4 M Ϫ1 . Even though the binding affinity of rBC2LCN to glycan a is relatively weak (K a of 2.5 ϫ 10 4 M Ϫ1 ), the affinity to podocalyxin is expected to be increased, because of the high density of the glycan ligands expressed on podocalyxin by a so-called "glycan cluster effect" [43]. Altogether, these results indicate that H type 3 is a most probable human pluripotency marker, which is expressed exclusively in undifferentiated iPS cells [38] and is recognized by rBC2LCN.
Podocalyxin has important roles in cell morphology, adhesion, and migration in a variety of tissues including kidney podocytes, hematopoietic progenitors, vascular endothelia, and a subset of neurons [44]. In the developing kidney, podocalyxin plays an essential role in the formation and maintenance of podocyte foot processes [44]. Podocalyxin-null mice fail to form foot processes and slit diaphragms and thus exhibit profound defects in kidney development and die within 24 hours of birth with anuric renal failure [45]. The ectopic expression of podocalyxin induces morphologic changes including actin recruitment and the formation of microvillus and foot process in a manner dependent of its extracellular domain [44]. Podocalyxin coats the secondary foot processes of the podocytes and functions to open the urinary filtration barrier by keeping adjacent foot processes separated by its negative charge. Podocalyxin is also expressed in the hematopoietic system and is involved in cell migration [44]. In addition, podocalyxin is abnormally expressed in subsets of breast, prostate, liver, pancreatic, and kidney cancer, as well as leukemia, and is likely involved in metastasis [44]. Although the biological functions of podocalyxin in stem cells and its relationship with pluripotency are largely unknown, it is likely that podocalyxin regulates the maintenance and morphol-ogy of stem cells as well [4]. It would be of interest to investigate how changes of glycan structures on podocalyxin affect its protein and/or cellular functions. Indeed, partial loss-of-function mutation in the gene encoding glycoprotein-N-acetylgalactosamine-3-␤-galactosyltransferase (C1GalT1), an enzyme essential for the synthesis of core1 (Gal␤1-3GalNAc), which is the precursor of H type 3 (Fuc␣1-2Gal␤1-3GalNAc), was reported to cause kidney diseases including distortion of the glomerular-tubular architecture [46]. In these mice, podocalyxin is the major underglycosylated protein, and its appropriate glycosylation was implicated to be essential for kidney functions. Therefore, it is likely that altered glycosylation affects both the chemical and physical properties of podocalyxin as well as its recognition molecules, leading to the modification and regulation of cell-cell communications, which is important for the maintenance of pluripotency.

CONCLUSION
We conclude that podocalyxin is the glycoprotein ligand of rBC2LCN in human iPS and ES cells. The carbohydrate antigens of rBC2LCN are expressed on O-glycans of podocalyxin, since alkaline hydrolysis greatly reduced the binding of rBC2LCN to human iPS cells and ES cells. rBC2LCN bound to an O-glycan carrying H type 3 epitope structure isolated from iPS cells, suggesting that H type 3 is a novel pluripotency glycan marker. Further studies will be crucial to understand the roles of glycans of podocalyxin for pluripotency and self-renewal of human iPS cells and ES cells.