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Keratinase Enzyme Production from Bacillus Licheniformis KP9 Isolated from Chicken Feathers

24 DOI: 10.37398/JSR.2021.650706 Abstract: The aim of the present study was to isolate keratinolytic bacteria from chicken feathers, since it is rich in keratin. Microbial keratinases have become biotechnologically important since they target the hydrolysis of highly rigid, strongly cross-linked structural polypeptide “keratin” recalcitrant to the commonly known proteolytic enzymes trypsin, pepsin and papain. The isolation of keratinolytic bacteria was performed by routinely used microbiological techniques and all the 11 isolates were screened for the keratinase production. The potent strain KP9 was characterized and identified by 16S r-RNA gene sequencing as Bacillus licheniformis KP9. The production of enzyme keratinase was studied by submerged fermentation process. The production of enzyme was optimized at various pH, temperature, incubation period and inoculum size. The maximum keratinase enzyme production by Bacillus licheniformis KP9 was recorded at pH 7.0, temperature 35C, 3% inoculum size and 48 h of incubation period.