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Time kinetics of the interaction of concanavalin A with splenic lymphocytes of normal AKR mice.

Interaction of concanavalin A (Con A) with splenic lymphocytes of normal AKR mice led to concentration-dependent biphasic changes in electrophoretic mobility. Kinetics of this interaction suggested that at high concentration Con A interacted in two steps. The first step led to redistribution of Con A receptors and increased EPM. In the second step additional receptors to Con A became accessible and their interaction with Con A reduced surface charge density. The kinetic data thus provide additional evidence for the existence of two sets of qualitatively different receptors for Con A on AKR lymphocytes.