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An RNA Polymerase II Complex Containing Capping Enzymes and Viral Oncoproteins

The aim of this work was to identify proteins from Adenovirus 2‐infected HeLa cell extracts that interact with the carboxyl‐terminal domain of the largest subunit of RNA polymerase II. First, a mammalian RNA polymerase II complex was isolated from Adenovirus 2‐infected HeLa cell extracts by affinity chromatography against the carboxyl‐terminal domain of the largest subunit of RNA polymerase II, followed by chromatography on a Mono S fast protein liquid chromatographic column. Second, the isolated complex was further characterized by Western blot analysis, the formation of a GMP‐protein complex, and transcriptional activity. The isolated complex contains general transcription factors, chromatin‐remodeling factors, histone acetyltransferases, Srbs, capping enzymes, and E1A viral oncoproteins. The RNA polymerase II complex is active in transcription when supplemented with recombinant transcription factor IIB.