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SARS-CoV-2 Seroprevalence among a Southern U.S. Population Indicates Limited Asymptomatic Spread under Physical Distancing Measures

This study suggests limited but accelerating asymptomatic spread of SARS-CoV-2. Asymptomatic infections, like symptomatic infections, disproportionately affected vulnerable communities in this population, and seroprevalence was higher in African American participants than in White participants. The low, overall prevalence may reflect the success of shelter-in-place mandates at the time this study was performed and of maintaining effective physical distancing practices among suburban populations. Under these public health measures and aggressive case finding, outbreak clusters did not spread into the general population.

IMPORTANCE This study suggests limited but accelerating asymptomatic spread of SARS-CoV-2. Asymptomatic infections, like symptomatic infections, disproportionately affected vulnerable communities in this population, and seroprevalence was higher in African American participants than in White participants. The low, overall prevalence may reflect the success of shelter-in-place mandates at the time this study was performed and of maintaining effective physical distancing practices among suburban populations. Under these public health measures and aggressive case finding, outbreak clusters did not spread into the general population.
KEYWORDS COVID-19, SARS-CoV-2, antibody, coronavirus, seroprevalence A cute virus infections typically induce a vigorous antibody response. The overwhelming majority of COVID-19 patients also produce antibodies, initially of the IgM isotype and shortly thereafter of the IgG isotype. Seroconversion rates approach 100% at 3 weeks after symptoms appear and persist for several months (1)(2)(3)(4). The composition, quality, median and maximal duration of the IgG response are unclear as the earliest cases occurred less than 7 months ago (5). Both presymptomatic and asymptomatic transmission events of SARS-CoV-2 have been reported (6)(7)(8)(9)(10)(11). It is difficult, however, to define asymptomatic transmission a priori, as the full spectrum of SARS-CoV-2-associated clinical symptoms is still evolving (12,13). The degree to which asymptomatic transmission contributes to population seroprevalence and eventual herd immunity is unknown and the subject of intense debate.
Determining the extent to which SARS-CoV-2 can be transmitted asymptomatically shapes COVID-19 disease modeling and informs public health interventions. Asymptomatic transmission represents a key biological property of this virus. Early serology studies for SARS-CoV-2 were conducted in densely populated, urban settings, such as Wuhan, China; Bergamo, Italy; or Boston, New York, Los Angeles, or Seattle in the United States (14,15). These studies focused on patients who recovered after severe clinical disease (4). They were conducted at hot spots of transmission, in urban settings with multiple independent introductory events and extended opportunities for rapid spread, such as multiple modes of public transport and international travel (16).
North Carolina (NC), in contrast, represents a largely suburban and rural population with limited public transport. The first case of COVID-19 was reported on 2 March 2020. As in other locales, symptomatic cases were concentrated among vulnerable populations, such as residents of retirement homes, prison inmates, and workers in meatprocessing plants. "Stay-at-home" directives were implemented 30 March to 8 May 2020. Statewide, all schools were ordered to close 14 March and did not reopen during the study period. As of 19 June, NC reported 49,840 cases and 1,197 deaths (NC Department of Health and Human Services [DHHS]) for a population of 10,488,084 people (2019 census), based primarily on nucleic acid testing (NAT) of suspected cases. Thus, the fraction of infected persons based on case counts for this area and calendar time using viral NAT is 0.5%.

RESULTS
This study evaluated two cohorts of asymptomatic patients. ScreenNC enrolled 2,973 asymptomatic participants across the University of North Carolina Health System (UNC Health), representing 267 different ZIP codes. These individuals sought medical care unrelated to COVID-19. Individuals with known COVID-19 symptoms, at screening or in their recent past, were excluded. ScreenNC2 tested 1,449 sera from patients in clinical care not related to COVID-19 or recruited to the study. No patient was tested more than once. ScreenNC enrolled 28 April to 19 June 2020, and ScreenNC2 sampled patients 3 March to 4 June 2020. Table 1 shows the demographic composition of the ScreenNC cohort compared to patients accessing UNC Health sites during the same time period in 2020 as well as in 2019, i.e., pre-COVID-19. Table 2 shows the age distribution. There were no differences between overall patient populations accessing UNC Health in 2020 compared to 2019.
When comparing the ScreenNC population and patient populations accessing UNC Health in 2020, there were some notable differences. ScreenNC enrolled fewer 80ϩyear-old patients who accessed care during the same calendar period (2.6% versus 7.4%), slightly more females (65.8% versus 63.6%), fewer Black or African American participants (13.3% versus 23.3%, P Յ 0.001 by Fisher exact test), and fewer Hispanic or Latino participants than the comparator UNC Health population (2.6% versus 4.9%, P Յ 0.001 by Fisher exact test) during the same calendar period. These differences were used in a model of adjusted seroprevalence.
ScreenNC identified 24 out of 2,973 (0.8%) positive participants, and ScreenNC2 identified 10 out of 1,449 (0.7%) positive participants. Black or African American participants had twice the unadjusted seropositivity rate of Whites (1.5% versus 0.7%). The analysis of other demographic factors was not possible due to the small number of positive cases. The unadjusted seroprevalence remained constant over time for the ScreenNC2 population but showed an upward, although imprecise, trend for the larger ScreenNC cohort ( Fig. 1 and Table 3).
The counts were adjusted for assay characteristics using the method of Rogan and Gladen (19) and cohort characteristics using direct standardization to yield a population

DISCUSSION
This study identifies a very limited seroprevalence of SARS-CoV-2 among asymptomatic individuals accessing the UNC Health system. There was a suggestion of accelerating asymptomatic spread of SARS-CoV-2 during the study period and cohort, i.e., the transmission among persons who never subjectively felt ill. The seroprevalence of less than 1% was lower than estimates from earlier studies, but in line with recent studies using high-accuracy tests (15,17). The seroprevalence for this low-density  community was lower than reported in a large study of convenience samples that focused around "hot spot" cities and did not explicitly exclude persons with past symptomatic infections (20). This result may reflect the success of shelter-in-place state mandates and maintaining effective physical distancing among suburban populations during the time frame of our study. It may also reflect the low population density and delayed introduction of the virus compared to population centers and travel nodes. Under these circumstances, early outbreak clusters did not expand far into asymptomatic patients, which indicates that continued viral NAT and aggressive case finding can curb SARS-CoV-2 spread. The data suggest that members of the Black community are disproportionately affected by the COVID-19 epidemic, here as demonstrated by asymptomatic acquisition of infection and in other studies as demonstrated by increased incidence of symptomatic cases and deaths. Significantly fewer members of the Black and Latinx communities participated in this study than accessed care in the same calendar time, which may have led to biased enrollment.
There are many possible explanations for the variation in population seroprevalence among different preliminary reports and published studies (14,17). Our study populations were derived from individuals accessing health care clinics and may not provide a population-representative study sample. It is very challenging to conduct random population sampling during a public health emergency when "stay-at-home" orders are in place. Prevalence of transmission, patterns of behavior, and characteristics of the sample population were time varying during the sample period. Logistical constraints, such as lack of personal protective equipment and the primacy of clinical care, limited study access. In NC, only persons with a valid medical reason and "essential workers" were exempted from the "stay-at-home" ordinance, and only those participated in the study. The degree to which underlying health conditions impact analytical assay sensitivity is currently unknown; for instance, ScreenNC2 included patients with immune suppression and thus likely impairments in their ability to generate high-titer antibodies.
Defining "asymptomatic" is a challenge and a limitation, as it is based on selfreported clinical symptoms of infection, the exact definition of which continues to evolve. Both symptom severity and awareness may differ among different communities. Third, there exists a wide variety of antibody tests, each with differing performance characteristics (21,22). These serological tests are validated on symptomatic, hospitalized patients, not asymptomatic cases. This study employed an EUA assay performed in a CLIA-certified laboratory on a venous blood sample, with demonstrated specificity to detect antibodies only to SARS-CoV-2, not to seasonal coronaviruses.
In general, a robust IgG response to SARS-CoV-2 is correlated with the presence of neutralizing antibodies for coronaviruses, but exactly which level of response and which antigen is a reliable predictor for protection has not been established (22). This study only uses the presence of IgG as a measure of exposure. The negative predictive value under any of the reported sensitivity/specificity characteristics is Ͼ99.5% for assumed population seroprevalence of Ͻ5%. The positive predictive value is 83.6% for an assumed seroprevalence of 5% and 48% for an assumed seroprevalence of 1%. This implies that perhaps even fewer individuals developed antibodies than reported. Alternatively, it is possible that IgG responses are lower in asymptomatic than symptomatic patients and decline over time (23).
In conclusion, based on the data presented in this study, it is unlikely (i) that "herd immunity" can be reached in the foreseeable future and (ii) that asymptomatic spread contributed a substantial number of infections. Taken together, these data suggest that early and rigorous shelter-in-place policies and physical distancing measures are important tools to suppress SARS-CoV-2 infection in the majority of the population.

MATERIALS AND METHODS
Enrollment. Patients enrolled in the study were recruited at nine outpatient clinical sites and two emergency department sites across the state associated with the UNC Health network. Upon arrival for SARS-CoV-2 Seroprevalence in North Carolina ® routine care or scheduled visits for enrollment into the study, patients performed a consent procedure that included reviewing recent COVID-19 clinical history using UNC IRB-approved questionnaires. Once consent was obtained, patients provided 5 ml of venous whole blood. This sample was collected by phlebotomists or nurses at each site and analyzed for the presence of SARS-CoV-2 antibody. Results were reported in the UNC medical record, and patients were notified of negative results via an electronic message. For those with a positive result, a physician was responsible for calling the patients and reviewing the results and answering follow-up questions. The ScreenNC protocol was approved by the UNC IRB (UNC IRB 20-0937, NCT04367740). ScreenNC2 samples were sera obtained from UNC patients for indications other than COVID-19. This protocol was also approved by the UNC IRB, which allowed for waiver of HIPAA and consent from these patients (UNC IRB 20-1308).
The comparator cohorts (2019 and 2020) were composed of all patients with any procedure seen in the project testing sites. These include two emergency room sites and multiple outpatient locations. Canceled appointments are excluded. Nineteen departments in the UNC Health System were identified, as some study sites comprise multiple departments. The 2019 cohort covers 15 February 2019 to 15 June 2019, and the 2020 cohort covers 15 February 2020 to 15 June 2020.
Detection of IgG antibody to SARS-CoV-2. IgG antibody to SARS-CoV-2 was detected with a microparticle chemiluminescence assay (Abbott Laboratories) on the Abbott Architect i2000SR immunoassay analyzer according to the manufacturer's protocol under EUA. The Abbott SARS-CoV-2 IgG assay utilizes microparticles coated with SARS-CoV-2 nucleocapsid protein. After washing, bound IgG was detected via addition of anti-human acridinium-labeled second-step antibody. Following a second wash step, pretrigger and trigger solutions were added and a chemiluminescent reaction was detected and reported in relative light units (RLU). The RLU generated is reflective of the amount of antibody bound to the microparticles. The sample RLU was compared to the assay-specific calibrator RLU to generate an index value (S/C). The presence of antibody was reflected by an index value of Ն1.4. Index values of Ͻ1.4 were classified as negative for SARS-CoV-2 IgG.
The Abbott SARS-CoV-2 IgG assay was evaluated in-house to assess sensitivity, specificity, and reproducibility. Sensitivity was determined by testing remnant serum samples from patients admitted to UNC Hospitals and confirmed to be infected by RT-PCR testing of nasopharyngeal swabs. Sera collected in the first (n ϭ 22), second (n ϭ 26), third (n ϭ 17), and fourth (n ϭ 23) weeks after disease onset were tested. Calculated sensitivities were 31.8%, 80.8%, 100%, and 100%, respectively. This pattern follows the known natural history of IgG antibody development, where titers take 1 to 2 weeks to reach detectable levels after primary infection. Specificity was determined by testing 277 sera. Two hundred thirty-eight of these were collected prior to 1 January 2020 (predating widespread U.S. COVID-19 cases), and 39 were collected after that date. Twenty-three of these 39 patients had nasopharyngeal swabs tested by SARS-CoV-2 RT-PCR and were negative. Additionally, 6 of the 39 samples were from individuals tested for endemic, seasonal coronavirus infection in respiratory secretions and were positive by PCR for the seasonal coronaviruses but not SARS-CoV-2. This group was important to include to establish the specificity of the IgG ELISA for SARS-CoV-2. The remaining 10 sera were from patients with IgM antibodies to cytomegalovirus (CMV) or Epstein-Barr virus (EBV). This set of 39 sera was not "pristine" sera but reflective of the general population in that it included sera with potentially interfering antibodies to other infectious agents. Of the 238 sera collected prior to 1 January 2020, 20 serum samples were from patients containing IgE antibodies to common respiratory allergens, 10 were from individuals with positive antinuclear antibodies, and 9 were from individuals with IgM antibodies to CMV or EBV. Additional sera were obtained from healthy laboratory staff (n ϭ 9), pre-solid organ transplant waitlisted patients (n ϭ 55), HIV-positive persons (n ϭ 6), and a healthy platelet donor cohort (n ϭ 129). In total, three serum samples in this set of 277 sera had a positive result. All three were from samples collected prior to 1 January 2020. The estimated specificity of the assay was 98.9%. Intra-assay precision (CV%), determined by performing the 10 replicate determinations of a positive sample, was 1.1%. The same sample was tested seven consecutive days and showed an interassay CV of 0.92%. Statistical analysis. Seroprevalence was estimated using the Rogan and Gladen estimator to adjust for assay sensitivity and specificity. Direct standardization was used to estimate seroprevalence in the UNC Health population. Confidence intervals were obtained using the nonparametric bootstrap percentile method. Specifically, within each stratum of age group, gender, and race, sample seroprevalence was estimated and weighted by the stratum's proportion in the population. The Rogan-Gladen estimate can be negative, so all estimates of seroprevalence were constrained to be within the interval [0,1]. For comparison, demographic data for patients accessing the screening locations during the time period 15 February 2020 to 15 June 2020 were used.