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Precision dispensing of small volumes of albumin solder for laser-assisted wound closure

Recent laser-tissue soldering work in our lab has demonstrated the feasibility of building a solder bond from individually coagulated small droplets using a precision pipette for the deposition of the solder droplets. This method of using small, precise volumes of solder to build a bond may result in stronger and more reproducible bonds than coagulating an equivalent large volume of solder all at once. We have investigated the technique further in this study. The solder was dispensed onto the intimal side of a bovine aorta substrate and irradiated with an 808nm diode laser. A bond was created across an incision in the tissue substrate by alternately dispensing and coagulating each small volume of solder, or by coagulating a single large equivalent volume. Acute strength analysis was performed on the solder bond. Future work will concentrate on testing a bench-top solder dispensing device and investigating the feasibility of turning the deice into a prototype tool for clinical applications.