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Hormonal control of eclosion of flies from the puparium.

Pharate flies (Sarcophaga bullata) very close to eclosion were injected with hemolymph from eclosing flies or various other preparations, kept at 10 degrees C for 24 hr, returned to room temperature, and tested for eclosion behavior (ongoing, attempted, or completed eclosion) during the first 30 min, the subsequent 2(1/2) hr, and after 51 hr. Flies injected with "eclosion" hemolymph showed significantly more eclosion activity during the first 30 min than noninjected controls, flies injected with hemolymph from larvae, or flies injected with Ringer solution.