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Effect of Adipocyte‐Conditioned Media and Phytochemicals on Galectin‐3 Expression in Rat Intestinal and Human Colon Carcinoma Cells

In a prior study, we demonstrated galectin‐3 (Gal‐3), a 31 KDa ?galactoside‐binding lectin family. This protein is reported to be involved in tumor metastasis and cellular transformation, has been found to be regulated by conditioned media (CM) collected from pre‐adipocyte, differentiated adipocyte, and macrophage cultures. In order to test hypothesis that obesity cause inflammation which, in turn, promotes colon carcinogenesis, we speculated that cytokines secreted by pre‐adipocyte, differentiated adipocyte and macrophage into conditioned media affected de novo synthesis of Gal‐3 in rat intestinal epithelial cells and human colon carcinoma cells such as HT‐29, HCT‐116. That is, this study was aimed to identifying cytokines (s) or phytochemicals which can regulate Gal‐3 expression. We speculated that phytochemical such as genistein, glyceollins, delphinidin, and daidzein enhanced the expression of Gal‐3 by CM collected from pre‐adipocyte, differentiated adipocyte and macrophage cultures in IEC‐6 cells and colon cancer cell lines. This study also showed that cellular level of galectin‐3 was reduced by some phytochemicals including genistein, glyceollins, delphinidin, and daidzein in IEC‐6 cells and HT‐29, HCT‐116 cells, suggesting their anti‐metastatic potential.