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Slicing and dicing viruses: antiviral RNA interference in mammals

Abstract To protect against the harmful consequences of viral infections, organisms are equipped with sophisticated antiviral mechanisms, including cell‐intrinsic means to restrict viral replication and propagation. Plant and invertebrate cells utilise mostly RNA interference (RNAi), an RNA‐based mechanism, for cell‐intrinsic immunity to viruses while vertebrates rely on the protein‐based interferon (IFN)‐driven innate immune system for the same purpose. The RNAi machinery is conserved in vertebrate cells, yet whether antiviral RNAi is still active in mammals and functionally relevant to mammalian antiviral defence is intensely debated. Here, we discuss cellular and viral factors that impact on antiviral RNAi and the contexts in which this system might be at play in mammalian resistance to viral infection.


Introduction
Metazoan organisms are constantly exposed to viruses and have evolved diverse mechanisms to combat the invaders. One group of mechanisms operates in a cell-intrinsic fashion, targeting viral nucleic acids and viral proteins for destruction and/or causing the premature shutdown or demise of infected cells to prevent them from serving as virus producers. Cell-intrinsic antiviral mechanisms are part of the innate immune system and include RNA interference (RNAi) and the interferon (IFN) system. The two systems operate very differently even though they can both be triggered by virally derived long double-stranded RNA (dsRNA) or highly base-paired single-stranded RNA (ssRNA). DsRNA can derive from the viral genome (in the case of a dsRNA virus) or from annealing of two strands of complementary RNAs, which are generated as RNA virus replication intermediates or DNA virus convergent transcripts. Highly based-paired ssRNAs are found in hairpins within viral genomes or viral transcripts and are generically referred to as dsRNA, a nomenclature that we retain here even if technically incorrect. Both types of dsRNA are largely absent from uninfected cells and act as hallmarks of viral infection to trigger innate antiviral immune responses.
In RNAi, long dsRNA is cleaved by the type III endoribonuclease Dicer into small interfering RNA (siRNAs) (Bernstein et al, 2001), RNA duplexes of 21-24 nucleotides (nts) in length, with 3 0 2-nt overhangs and a 5 0 mono-phosphate and a 3 0 hydroxyl group on both strands (Fig 1) (Hamilton & Baulcombe, 1999;Zamore et al, 2000;Elbashir et al, 2001b,a). One strand of each siRNA duplex is bound by an Argonaute (Ago) protein, which, together with accessory proteins, forms the RNA-induced silencing complex (RISC) and mediates the endonucleolytic cleavage ("slicing") of complementary target RNAs (Hammond et al, 2000;MacRae et al, 2008). Of the four Ago proteins encoded by the mammalian genome, only Ago2 has catalytic activity and is essential for target slicing and RNAi (Liu et al, 2004;Meister et al, 2004;Swarts et al, 2014;Sheu-Gruttadauria & MacRae, 2017). However, all four Ago proteins are involved in an RNAi-related process, the microRNA (miRNA)mediated gene silencing pathway, which does not involve slicing but translation inhibition and/or mRNA degradation (Bartel, 2009;Jonas & Izaurralde, 2015). Notably, Dicer is also involved in miRNA biogenesis. Vertebrates and nematodes possess a single Dicer that generates both siRNA and miRNAs while most invertebrates express two Dicer proteins. For example, in Drosophila melanogaster, dmDcr-1 is dedicated to the miRNA pathway while dmDcr-2 performs antiviral RNAi .
Third, many plant and insect viruses encode viral suppressors of RNAi (VSRs) that interfere with distinct steps of the RNAi pathway, demonstrating the selection pressure imposed by this antiviral system (Pumplin & Voinnet, 2013;Bronkhorst & van Rij, 2014;Csorba et al, 2015).
In contrast, in chordate cells, including mouse and human cells, dsRNA and other nucleic acids associated with viral infection trigger cytosolic innate immune pathways that induce the production of type I IFNs (mainly IFNa and IFNb) and type III IFNs (IFNk) (Goubau et al, 2013;Schneider et al, 2014;Wu & Chen, 2014;Schlee & Hartmann, 2016) (Fig 1). These key antiviral cytokines are then secreted and act in an autocrine and paracrine manner by binding to their cognate receptors, i.e. the ubiquitously expressed IFNa/b receptor (IFNAR) and the epithelial cell type-restricted type III IFN receptor (IL-28R), which signal to induce hundreds of interferon-stimulated genes (ISGs) (Schneider et al, 2014). The proteins encoded by these ISGs limit viral replication directly (Schoggins et al, 2011) and serve to enhance adaptive immune responses to the virus (de Veer et al, 2001;Iwasaki & Medzhitov, 2010). For example, the dsRNA-dependent protein kinase R (PKR) is activated by cytosolic dsRNA and phosphorylates and inactivates the eukaryotic translation initiation factor 2 a (eIF2a), resulting in translational arrest and thwarting the production of both viral and host cell proteins (Pindel & Sadler, 2011 demise of the infected cell, further undermining the ability of the virus to propagate. Virally derived long dsRNA or highly based-paired RNA is detected in the cytosol of mammalian cells by RIG-I like receptors (RLRs), which include RIG-I (retinoic acid-inducible gene I), MDA5 (melanoma differentiation factor 5) and LGP2 (laboratory of genetics and physiology 2) (Fig 1). RIG-I recognises based-paired ds or ssRNA with a di-or triphosphate (5 0 PP/5 0 PPP) at its 5 0 extremity (Hornung et al, 2006;Pichlmair et al, 2006;Schlee et al, 2009;Schmidt et al, 2009;Goubau et al, 2014), such as found in the genomes of influenza virus, Sendai virus and reovirus (Baum et al, 2010;Rehwinkel et al, 2010;Weber et al, 2013;Goubau et al, 2014). MDA5 triggers comprise long dsRNAs that accumulate during infection with certain viruses such as picornaviruses and reovirus (Gitlin et al, 2006;Kato et al, 2006;Weber et al, 2006;Pichlmair et al, 2009;Feng et al, 2012). RIG-I and MDA5 contain two tandem N-terminal CARDs (caspase activation and recruitment domains) that mediate downstream signalling via the adaptor protein MAVS (mitochondrial antiviral signalling protein), leading to activation of the transcription factors IRF3, IRF7 (interferon regulatory factors 3 and 7) and NFj-B (nuclear factor kappa-lightchain enhancer of activated B cells). These transcription factors drive the expression of type I and type III IFNs and can directly induce some ISGs.
LGP2 lacks CARDs and is unable to induce signalling via MAVS. It is thought to act by modulating responses by the other RLRs (Bruns & Horvath, 2014;Bruns et al, 2014;Parisien et al, 2018).
Thus, plants and invertebrates lack an IFN system and rely on antiviral RNAi to defend against viruses. In contrast, vertebrates have adopted the IFN system for cell-intrinsic antiviral defence and are thought to have abandoned antiviral RNAi even though they have retained the RNAi machinery and utilise it for miRNA generation and function. Recently, a number of studies have started to question whether the primordial antiviral function of RNAi has truly been abandoned by mammalian cells or whether it can constitute a physiologically relevant antiviral system that complements the IFN pathway. This has become an area of controversy, with some investigators suggesting that RNAi can be a relevant means of cellintrinsic restriction to virus infection in mammals while others argue that it is an epiphenomenon with no role in antiviral resistance (Cullen et al, 2013;Cullen, 2014;Ding & Voinnet, 2014;tenOever, 2014tenOever, , 2017Jeffrey et al, 2017). In this review, we address this controversy and summarise current understanding of antiviral RNAi pathways in mammals and reflect on the possible contexts in which it might play a role.

Detection of dsRNAi in mammalian cells with attenuated IFN responses
To evaluate the possible existence of antiviral RNAi in mammals, it is useful to consider studies that are exempt from virus-dependent variables such as expression of VSRs. Therefore, we first discuss studies that use synthetic long dsRNA, composed of two perfectly complementary strands, to trigger RNAi, termed here long dsRNAmediated RNAi (dsRNAi). This process depends on the successive processing of long dsRNA into a pool of siRNAs and is distinct from RNAi induced experimentally by the introduction of siRNAs (which bypasses the Dicer machinery) (Caplen et al, 2001;Elbashir et al, 2001a) or of short hairpin RNAs (shRNAs, which resemble the structure of pre-miRNAs) (Brummelkamp et al, 2002;Paddison et al, 2002b;Bartel, 2004).
Long dsRNA-mediated RNAi was first described in C. elegans (Fire et al, 1998) followed by Drosophila, Trypanosoma brucei, planarians and plants (Kennerdell & Carthew, 1998;Ngô et al, 1998;Waterhouse et al, 1998;Sánchez Alvarado & Newmark, 1999). In mammalian cell lines, long dsRNA had either no effect or displayed a non-sequence-specific effect, consistent with activation of the IFN system (Caplen et al, 2000;Elbashir et al, 2001a). Yet, in preimplantation embryos, as well as in oocytes, embryonic stem cells (ESCs) and embryonal carcinoma (EC) cell lines, the introduction of long dsRNA targeting endogenous genes caused a specific reduction in gene expression and induction of phenotypes comparable to those of null mutants, without causing cell death or translational arrest (Svoboda et al, 2000;Wianny & Zernicka-Goetz, 2000;Billy et al, 2001;Yang et al, 2001;Paddison et al, 2002a). The sequence-specific silencing induced by long dsRNA in oocytes and ESCs/ECs correlated with a relative inability of these cells to produce and/or respond to IFN (Burke et al, 1978;Francis & Lehman, 1989;Stein et al, 2005;D'Angelo et al, 2016;Wu et al, 2018), which suggested that dsRNAi might be active in mammalian undifferentiated cells but masked or inhibited by the IFN system in differentiated cells.

Antagonism between the IFN response and dsRNAi
Antagonism between the IFN system and dsRNAi was formally tested in somatic cells genetically deficient in MAVS or IFNAR (Maillard et al, 2016). In such cells, introduction of dsRNA resulted in Dicer-dependent accumulation of siRNAs and Ago2-dependent sequence-specific gene silencing (Maillard et al, 2016). A subsequent study showed that the IFN system actively inhibits dsRNAi at least in part through induction of LGP2, which binds Dicer and inhibits processing of long dsRNA into siRNAs (Van der Veen et al, 2018) (Fig 2). In that study (Van der Veen et al, 2018), LGP binding to Dicer did not impact the biogenesis of two household miRNAs although LGP2 has also been reported to interact with the Dicer cofactor TRBP (HIV TAR RNA-binding protein) and inhibit the processing of a subset of TRBP-bound miRNAs (Komuro et al, 2016;Takahashi et al, 2018). Whether LGP2 additionally inhibits dsRNAi via TRBP remains to be addressed.
It is unclear why somatic cells should inhibit dsRNAi during an IFN response. A clue may come from the observation that mammalian cells stably expressing Drosophila dcr-2 to artificially boost dsRNAi have impaired induction of IFN upon treatment with poly(I:C), a dsRNA analog (Girardi et al, 2015). Viral infection or treatment with poly(I:C) also induces poly-ADP-ribosylation of Ago2 and other RISC components, which inhibits RISC activity and causes a relief in miRNA-mediated repression of some ISGs (Seo et al, 2013). Perhaps, inhibition of Dicer and RISC is essential for effective stimulation of the IFN pathway, in part by preventing loss of dsRNA substrates for RLR activation. Preservation of dsRNA in infected cells may also ensure that the activity of antiviral proteins encoded by ISGs is not compromised. For example, PKR requires dsRNA of > 30 nts to dimerise and become active for translational repression (Husain et al, 2012). Dicer-mediated cleavage of long dsRNA could starve the cell of substrates for PKR activation or, more likely, lead to accumulation of 21-22 nt siRNA duplexes that would "quench" PKR monomers, blocking substrate-dependent dimerisation.
Intrinsic inefficiency of mammalian Dicer in processing long dsRNA DsRNAi in mammalian cells is further influenced by the molecular properties of its central component: Dicer. This large multi-domain enzyme comprises an N-terminal DExD/H helicase domain (containing an ATPase site) followed by a small domain of unknown function (DUF283), a Piwi Argonaute Zwille (PAZ) domain, two tandem RNAse III domains and a C-terminal dsRNA-binding domain (dsRBD - Fig 1). The PAZ domain binds the 3 0 2nt-overhangs found at the extremity of dsRNA substrates, while the RNAse III domains each mediate the cleavage of one strand of the RNA duplex. In vitro studies revealed that human Dicer (hDcr) processes long dsRNA into siRNAs less efficiently than pre-miRNA into miRNAs (Ma et al, 2008;Chakravarthy et al, 2010). Deletion or partial proteolysis of the helicase domain increases rate of dsRNA cleavage, while only modestly affecting the cleavage of pre-miRNAs (Provost et al, 2002;Zhang et al, 2002;Ma et al, 2008). Similarly, a deletion mutant of hDcr lacking nearly the entire helicase domain displayed an enhanced ability to process endogenously transcribed long dsRNA and long hairpin RNAs into siRNAs and conferred dsRNAi activity to engineered cells (Kennedy et al, 2015). Finally, mouse oocytes, in which dsRNAi is active, express a shortened isoform of Dicer (Dicer O ) that lacks the N-terminal helicase domain and processes endogenous or ectopically expressed long hairpin RNAs more efficiently (Flemr et al, 2013). Together, these data suggest that the helicase domain of Dicer inhibits its catalytic activity for long   dsRNA and that its incorporation into the mature enzyme might be regulated by alternative transcription. However, expression of Dicer O has not been detected outside mouse germ cells and expression of truncated Dicer isoforms in humans has been reported only in certain cancer cell lines (Potenza et al, 2010;Hinkal et al, 2011;Cantini et al, 2014). An alternative possibility is that modulation of inhibition by the helicase domain could come about not through alternative transcription but through the activity of Dicer-associated proteins. Structural studies show that the co-factors TRBP and PACT (Protein Activator of PKR) induce a conformational change in the Dicer helicase domain (Taylor et al, 2013) that could mimic the effect of deletion Ma et al, 2008;Chakravarthy et al, 2010;Ota et al, 2013). The exact role and cellular context in which TRBP and PACT might modulate the ability of Dicer to process long dsRNA in vivo remains to be explored.  et al, 2013). Finally, in IFN-defective somatic cells, introduction of long dsRNA conferred sequence-specific protection from viral challenge dependent on the "slicing" activity of Ago2 (Maillard et al, 2016). Together, these studies show that, if siRNAs are provided directly (bypassing Dicer processing) or are generated from substrates in the absence of an IFN response (shRNAs or dsRNAs in IFN-defective cells), RISC can access and target viral RNAs to limit viral accumulation. As such, much of the current debate on the role of antiviral RNAi in mammalian cells ultimately centres on the question of whether, during a viral infection, viRNAs are ever produced in sufficient amounts to engage the latent antiviral activity of RISC and exert an antiviral effect.

Viral determinants of antiviral RNAi
viRNA production and antiviral RNAi in mammalian cells viRNAs have key features: (i) a discrete length of~22 nt, (ii) extremities with 3 0 2nt overhangs and (iii) a strand derived from the positive (+)-sense viral RNA and a complementary strand commonly derived from the negative (À)-sense viral RNA or, less frequently, from intramolecular base pairing of viral ssRNA. Initial attempts failed to detect viRNAs in mammalian cells infected with viruses (Pfeffer et al, 2005;Lin & Cullen, 2007), yet the recent emergence of high-throughput sequencing has allowed the question to be reexplored more thoroughly. In contrast, deep sequencing of mESCs infected with the picornavirus encephalomyocarditis virus (EMCV) revealed the accumulation of viral reads with a specific peak at 21-23 nt and reads that mapped within the first 200-nt of the EMCV genome and, to a lesser extent, to the 3 0 end and that, importantly, derived in equal parts from the (+) strand and (À) strand (Maillard et al, 2013). The sequences formed perfectly paired duplexes with 3 0 2-nt overhangs and were produced in a phase pattern indicative of successive cleavage by Dicer. Finally, the duplexes could be shown to associate with Ago2 and require Dicer for their generation, thereby fulfilling all criteria for bona fide viRNAs (Maillard et al, 2013). Interestingly, production of these viRNAs by mESCs was greatly reduced upon cell differentiation (Maillard et al, 2013), in line with the aforementioned studies reporting little viRNA generation in differentiated somatic cells.
Thus, in some IFN-deficient cells, including ESCs, infection with viruses allows for viRNA production. Can it elicit a protective RNAidependent response? Early work suggested that knockdown of Dicer in Vero cells, an African green monkey cell line that lacks IFN-a and IFN-b genes (Diaz et al, 1988), causes a modest increase in virus production upon IAV infection (Matskevich & Moelling, 2007). In contrast, later reports found that absence of Dicer in HEK 293T cells and/or in mouse embryonic fibroblasts did not impact the accumulation of flaviviruses (DENV, WNV, YFV), alphaviruses (SINV, Venezuelan equine encephalitis virus [VEEV]), IAV, measles virus, HIV and reovirus (Shapiro et al, 2010;Bogerd et al, 2014). Similarly, in engineered cells overexpressing an artificial Dicer that lacks the helicase domain, infection with IAV or poliovirus led to lowlevel accumulation of viRNAs, which were loaded onto RISC but had little impact on replication (Kennedy et al, 2015). Finally, ª 2019 The Authors The EMBO Journal 38: e100941 | 2019 expression of a slicing-deficient Ago2 mutant in Ifnar1 À/À mouse embryonic fibroblasts did not impact infection with Semliki Forest virus (SFV), reovirus or IAV (Maillard et al, 2016). The overall message from those studies is that, even in a context permissive for dsRNAi, viRNA production from viral replication intermediates is too weak to inhibit viral infection. Replication of (+)-sense RNA viruses often occurs within membranous structures (replication factories), whereas RNAs generated during replication of (À)-sense RNA viruses rapidly associate with nucleocapsid proteins (Conzelmann, 1998;Romero-Brey & Bartenschlager, 2014). In addition, the 5 0 extremities of certain viral genomes (and replication intermediates) can display various modifications, including a 7methylguanosine (Cap) structure, a covalently linked protein (e.g. Vpg, viral protein genome-linked), highly structured regions or 2-3 phosphates (Fig 2). Whether these features prevent efficient access of Dicer to viral RNA is unknown although it is worth remembering they do not prevent antiviral RNAi in insect cells. A key issue might therefore be antagonism of RNAi by VSRs. This will be discussed in the next section.

VSRs in mammalian viruses
Several proteins encoded by mammalian viruses display VSR activity (1; 2). Expression of influenza virus NS1, vaccinia virus E3L, reovirus r3 and Nodamura virus (NoV) B2 proteins inhibits RNAi in plants and/or insect cells (Lichner et al, 2003;Bucher et al, 2004;Delgadillo et al, 2004;Li et al, 2004 2006b;Haasnoot et al, 2007;Chen et al, 2008;de Vries et al, 2009;Karjee et al, 2010;Fabozzi et al, 2011;Kakumani et al, 2013;Cui et al, 2015;Samuel et al, 2016;Qiu et al, 2017). Most viral proteins identified thus far that display VSR activity share the ability to bind dsRNA and mutations that affect their dsRNA-binding domain block VSR activity, arguing that their principal mode of action is sequestration of dsRNA from Dicer (Table 1). As dsRNA is a potent inducer of the IFN pathway, most of these VSRs also act as IFN antagonists (García-Sastre, 2017). It is therefore unclear whether these viral proteins specifically evolved to block RNAi or whether their VSR activity is a byproduct of their role as IFN antagonists (Cullen, 2006). However, some VSRs may function through mechanisms other than dsRNA sequestration (Kakumani et al, 2013), including binding to components of the RNAi pathway: e.g. Ebola virus VP35 and VP30 proteins interact with Dicer co-factors TRBP and PACT, while HCV core associates with Dicer (Table 1) (Wang et al, 2006b;Chen et al, 2008;Fabozzi et al, 2011). Whether these interactions contribute to VSR activity is unclear. Finally, adenovirus VA1s are small, highly structured RNAs that inhibit shRNA-mediated RNAi by acting as decoy substrates for Dicer, RISC and exportin 5 (required for nuclear export of pre-miRNAs and shRNAs) (Lu & Cullen, 2004;Andersson et al, 2005). Despite the evidence that many viral proteins from mammalian viruses can act as VSRs in overexpression (i.e. gain-of-function) studies, there are relatively few loss-of-function studies that show that they actively suppress mammalian antiviral RNAi defence. Persuasive experiments have been done with NoV, a member of the Nodavirus family. Nodaviruses express B2 proteins, which bind long dsRNA and siRNAs in vitro and associate with replication intermediates and viRNAs in infected cells (Chao et al, 2005;Lu et al, 2005;Sullivan & Ganem, 2005;Aliyari et al, 2008). B2 proteins act as potent VSRs in insect cells (Wang et al, 2006a;Aliyari et al, 2008). Notably, B2-deficient NoV (NoV DB2) also replicates less efficiently than parental NoV in mESCs but its accumulation is rescued in mESCs lacking all Ago genes (Maillard et al, 2013). In suckling mice, NoV DB2 is highly attenuated and induces accumulation of viRNAs . viRNAs are also detected, although to a lesser degree, upon infection of somatic cells (BHK-21) with NoV DB2, but not with NoV WT . Altogether, these data suggest that the ability of Dicer to process NoV replication intermediates is actively antagonised by the B2 protein.
The NS1 protein from IAV also displays VSR activity when expressed in plants and insect cells (Bucher et al, 2004;Delgadillo et al, 2004;Li et al, 2004). In mammalian cells, NS1 is ineffective against RISC-loaded siRNAs (Kok & Jin, 2006;Haasnoot et al, 2007;de Vries et al, 2009;Kennedy et al, 2015) but infection of human and African green monkey cells with IAV DNS1 but not IAV WT yields readily detectable levels of canonical viRNAs derived from the termini of both strands of the eight viral RNA segments (Li et al, 2016;Tsai et al, 2018). It has been reported that IAV DNS1, and to a lesser extent parental wild-type IAV, VSV and EMCV, replicates more extensively in mouse embryonic fibroblasts expressing a slicing-deficient Ago2 mutant (Li et al, 2016). However, in other studies, loss of RNAi components did not cause an increase in replication of IAV DNS1 (Maillard et al, 2016;Tsai et al, 2018). Interestingly, IAV engineered to express an shRNA or a miRNA targeting a viral gene or a reporter gene integrated in the viral genome, respectively, was attenuated compared to non-targeting controls (Benitez et al, 2015). This restriction was Dicer-dependent but independent of NS1 suggesting that, in the context of an infection, NS1 0 s VSR activity inhibits viRNA production from genome segments but not from short dsRNA hairpins (Benitez et al, 2015;Li et al, 2016;Tsai et al, 2018).
Finally, the HEV71-encoded protein 3A inhibits shRNA-mediated silencing in mammalian cells, as well as antiviral RNAi in insect cells, and suppresses Dicer-mediated biogenesis of siRNAs by binding and sequestering long dsRNA in vitro (Qiu et al, 2017). A point mutation that inactivates 3A's VSR activity reduces viral replication in somatic cells and in suckling mice. Concomitantly, canonical viRNAs derived from both strands of the 5 0 terminal region of the HEV71 genome are produced, loaded into RISC and able to silence a reporter bearing complementary sites (Qiu et al, 2017). Interestingly, the absence of Dicer increases HEV71 accumulation in infected cells despite the presence of an intact IFN pathway, suggesting that, in this case, antiviral RNAi could function irrespective of the IFN system (Qiu et al, 2017).

Niches for antiviral RNAi?
The generally observed antagonism between the IFN response and dsRNAi suggests that antiviral RNAi may be especially important in cellular niches in which the induction of or the response to IFN is limited. One of those niches might be stem cells. Pluripotent stem cells do not produce IFN upon viral infection or exposure to . Thus, pluripotent stem cells may be forced to rely on IFN-independent mechanisms to combat virus infections. These may include the ability to constitutively express some ISGs that confer an efficient and permanent antiviral state (Wu et al, 2018). In this scenario, antiviral RNAi would constitute an additional mechanism to protect the integrity and function of tissue stem cells in the face of virus infection and thereby contribute to tissue maintenance, repair and regeneration (Xia et al, 2018). Notably, the ability of a virus to infect stem cells might not be needed for its propagation and, therefore, stem cell-intrinsic antiviral RNAi would benefit the host but not impact on virus transmission.

Future directions in antiviral RNAi
In line with other facets of immunity, it is likely that antiviral RNAi is highly tuneable and that it operates in conjunction with multiple other mechanisms of defence. Further studies are clearly needed to disentangle the complex web that regulates dsRNAi in mammals and to understand its ability to act as a cell-intrinsic mechanism of antiviral defence.