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UVA-induced degradation of 8-hydroxyguanine in oligonucleotide and the effect on its activities in yeast oligonucleotide transformation assay.

UVA-induced conversion of 8-hydroxyguanine in oligonucleotides was studied. By irradiation with 334 nm UVA light, 8-hydroxyguanine was completely changed to unknown compounds. Monomeric nucleoside may be much less labile to UVA. Mutagenic specificities of 8-hydroxyguanine were investigated using yeast oligonucleotide mutation assay. UVA irradiation moderately reduced the activity of the oligonucleotides.