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Bound water in aqueous mono-and disaccharide solutions: A depolarization thermocurrent study

We report in this paper on the dielectric behaviour of frozen aqueous solutions of mono-and disaccharides studied by the depolarization thermocurrent (DTC) method in the temperature range of 85–250 K and over a wide range of concentrations (0.0003–1.5 mol/l), to obtain information on the state of water in the solutions. The dielectric behaviour of aqueous oligosaccharide solutions was studied systematically by Tait et al.[1] and by Sugget and Clark [2] at temperatures of 5 and 25 C in the GHz frequency range. The main problem with such measurements is that the analysis of the measured frequency dependence of the complex permittivity in terms of contributions of free (bulk) water, hydration (bound) water and solute can never be completely unequivocal. We pointed out in a previous paper [3] that one may avoid such problems in the analysis of the measurements by using the DTC method. We showed in that paper that in frozen aqueous solutions of glucose there is a continuous transition from hydration to free H2O molecules. In this paper we extend our measurements to other monosaccharides and to disaccharides.