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[Effect of carbon dioxide on the initial rate of the isocitrate and malate dehydrogenase reactions in rat liver].

The rate of reactions catalyzed by the NADP-dependent isocytrate dehydrogenase and malate dehydrogenase soluble fraction of the rat liver was studied as affected by carbonic acid. The reaction initial rate for both enzymes was determined under normal conditions and at physiological concentrations of carbonic acid. The initial rate of the direct isocytrate dehydrogenase reaction is established to decrease by 20, 35 and 57% at the carbonic acid concentrations 20, 40 and 60 mM. At the same time the initial rate of the malate dehydrogenase reactions increases by 51, 86 and 104% at the same levels of carbonic acid.