{'fraction_non_alphanumeric': 0.031355901972675064, 'fraction_numerical': 0.015838476081760783, 'mean_word_length': 4.760016437230327, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Overexpression of Batf induces an apoptotic defect and an associated lymphoproliferative disorder in mice

Activator protein-1 (AP-1) is a dimeric transcription factor composed of the Jun, Fos and Atf families of proteins. Batf is expressed in the immune system and participates in AP-1 dimers that modulate gene expression in response to a variety of stimuli. Transgenic (Tg) mice overexpressing human BATF in T cells were generated using the human CD2 promoter (CD2-HA (hemagglutinin antigen) - BATF). By 1 year of age, over 90% of the mice developed a lymphoproliferative disorder (LPD). The enlarged lymph nodes characteristic of this LPD contain a polyclonal accumulation of T cells with a CD4+ bias, yet efforts to propagate these tumor cells in vitro demonstrate that they do not proliferate as well as wild-type CD4+ T cells. Instead, the accumulation of these cells is likely due to an apoptotic defect as CD2-HA-BATF Tg T cells challenged by trophic factor withdrawal in vitro resist apoptosis and display a pro-survival pattern of Bcl-2 family protein expression. As elevated levels of Batf expression are a feature of lymphoid tumors in both humans and mice, these observations support the use of CD2-HA-BATF mice as a model for investigating the molecular details of apoptotic dysregulation in LPD.

Activator protein-1 (AP-1) is a dimeric transcription factor that is comprised of ubiquitous and tissue-specific basic leucine zipper proteins of the Jun, Fos, Maf and Atf families of proteins. 1 In response to many stimuli, AP-1 component expression and DNA-binding activity are induced rapidly in cells and result in both the positive and negative regulation of gene expression. 2 In lymphocytes, AP-1 activity increases following B-cell and T-cell receptor (TCR) engagement and participates in a transcription factor network that regulates the expression of genes associated with lymphocyte activation and differentiation. 2,3 The AP-1 protein Batf is expressed in both T and B cells and although there is compelling evidence to suggest that AP-1 dimers in which Batf participates are transcriptionally inactive, 4,5 the recent identification of Batf genetic targets in vivo indicates that Batf also may function as a positive regulator of gene expression. 6,7 The role for Batf in the development and differentiation of lymphocytes has been investigated by several groups using T-cell-specific Batf transgenic (Tg) 8,9 and systemic Batf knock-out mice. 6,7,10,11 Results show that increased expression of Batf in T cells impacts signaling important to the proper development of thymic NKT cells 8,9 and that the loss of Batf has a dramatic impact on the differentiation of CD4 þ , IL-17-producing T-helper cells (Th17) and T-follicular T-helper cells (Tfh) in the periphery. 7,10 Additionally, Batf null animals possess an intrinsic B-cell defect that cooperates with the Th17 and Tfh-cell deficiencies to inhibit antibody responses to both T-cell-dependent and T-cell-independent antigens. 6,10 In conjunction with these studies, an analysis of gene expression changes in Batf null lymphocytes identified several genes in T cells and B cells whose expression is correlated with the binding of Batf to specific gene regulatory regions. 6,7 Precisely how the Batf protein directs the transcription of these target genes, however, is not known.
Previous studies on the function of Batf in vivo have focused primarily on phenotypes that are apparent in the mouse immune system by 4 months of age. In this study, we have utilized a line of Tg mice in which the human BATF protein is expressed constitutively in both thymic and peripheral T cells to test the impact of long-term, chronic BATF overexpression (and AP-1 mis-regulation) on T-cell maturation and homeostasis. Remarkably, we found that by 12 months of age, the vast majority of these animals develop lymphoid tumors consisting of a polyclonal population of T cells. Our studies demonstrate that this lymphoproliferative disorder (LPD) is not associated with an increase in the proliferative capacity of the T cells, but rather with an impaired response to an apoptotic stimulus. Given the observation that increased BATF expression is a feature of secondary neoplasias in human Hodgkin's lymphoma 12 and of MuMLV-induced T-cell lymphoma in mice, 13 the BATF Tg mice described herein represent a useful model for investigating the role of AP-1 mis-regulation in LPD.

Results
Tg mice expressing hemagglutinin antigen (HA) -epitope tagged human BATF protein in T cells (CD2-HA-BATF) have been described previously in conjunction with a study that demonstrated a direct role for BATF in regulating thymocyte proliferation in vitro. 14 For the current study, it was important to establish that the CD2-HA-BATF transgene continues to be expressed in peripheral T cells and that TCR signaling in Tg T cells remains intact and properly induced by AP-1 complexes containing BATF. Splenocytes from Tg, non-Tg (WT) littermates and Batf null mice were stimulated in vitro with anti-CD3 and anti-CD28 antibodies. Protein extracts were prepared and immunblotted using anti-BATF antiserum. Results show a robust overexpression of BATF protein in the Tg cells (Figure 1a). Nuclear extracts were prepared from resting and stimulated WT and Tg splenocytes and used for electrophoretic mobility shift assays (EMSA) to detect the presence of protein complexes competent for binding consensus AP-1 DNA (Figure 1b). In the absence of stimulation, the Tg nuclear extract showed increased AP-1 activity resulting from the formation of DNA-binding heterodimers containing human BATF and the Jun proteins expressed in resting T cells. Following stimulation, the endogenous AP-1 factors, including Jun, Fos and Batf, are upregulated in both WT and Tg cells and the DNA-binding activity in these extracts increases accordingly. This DNAbinding activity is identified as AP-1 by successful competition with a 100-fold excess of unlabeled AP-1, but not SP-1, DNA. The presence of BATF is demonstrated by the higher molecular weight complexes detected following the addition of polyclonal anti-BATF or monoclonal anti-HA antiserum to the reaction (Figure 1b, complexes 1 and 2, respectively). These results validate use of the Tg, CD2-HA-BATF mouse model to study the long-term impact of BATF expression on peripheral T-cell function.
Initial characterization of the effects of CD2-HA-BATF expression on peripheral lymphoid tissues was performed using histology and revealed a significantly reduced area of white pulp in Tg spleens (Figure 1c). This reduction was quantified to reveal that the total follicle area in Tg spleens was roughly 75% of WT controls (Figure 1c). To profile the splenic lymphocyte populations in CD2-HA-BATF mice, single-cell suspensions from 8-12-week-old mice were analyzed by flow cytometry. Although the total cellularity of Tg spleens is reduced compared with WT littermate controls (Figure 1d, left panel), the percentage of T cells in the spleens of Tg mice is increased (Figure 1d, center panel). Furthermore, Tg T cells show a statistically significant bias toward CD4 þ cells (Figure 1d, right panel). These findings are consistent with the reduced percentage of splenic CD4 þ T cells noted previously in Batf null (Batf DZ/DZ ) mice. 10 In order to determine how BATF overexpression influences T-cell homeostasis, CD2-HA-BATF Tg mice, along with their WT littermates, were allowed to age for up to 14 months. A significant decrease in body weight was observed in male Tg mice, whereas the average weight of female Tg mice was not reduced significantly compared with WT controls (Figure 2a). Upon necropsy, 91.6% of aged Tg mice of both sexes exhibited gross peripheral lymphadenopathy in at least one lymph node (Figure 2b). The affected nodes presented as single or multiple discrete tumors. Mesenteric nodes were most commonly affected by lymphadenopathy; however, disease also was observed in other peripheral lymph nodes and spleen. In addition to the gross lesions, microscopic evidence of lymphoid infiltrate was detected in the liver and pancreas of all tumor-bearing CD2-HA-BATF mice. Only three WT mice showed signs of a similar gross and/or microscopic disease at necropsy.
To evaluate the kinetics of tumor formation in CD2-HA-BATF mice, a longitudinal magnetic resonance imaging (MRI) study was conducted for 10 months during which time 11 Tg and 10 WT littermate control mice were imaged monthly beginning at 4 months of age. The results confirmed that the tumor phenotype was an exclusive feature of older Tg animals, as the median age at earliest tumor detection by this noninvasive method was 10 months (Figure 2c).
Histological characterization of the tumor tissue from CD2-HA-BATF mice revealed a heterogeneous population of small and intermediate sized lymphocytes, which completely disrupted the follicular architecture of the lymph node ( Figure 2d, upper panels). Immunohistochemistry (IHC) identified the predominant tumor-cell population as CD3 þ T cells although low, but variable, numbers of CD79a þ B cells were found throughout the affected nodes ( Figure 2d, middle panels). Importantly, the Tg HA-BATF protein was detected in a high percentage of the tumor cells by IHC (Figure 2d, lower panels) and flow cytometry on individual tumor-cell suspensions detected anti-HA reactive BATF protein, on average, in 68% of the CD3 þ T cells analyzed (Figure 2e).
To augment the IHC results, cell suspensions from several tumors were analyzed using flow cytometry to quantify the relative percentages of B versus T lymphocytes and to quantify any bias toward CD4 þ or CD8 þ cells. Pooled singlecell suspensions from the lymph nodes of aged WT mice were used as controls. As shown in Figure 3a, the tumors are composed primarily of T cells (upper panels) and display a bias towards CD4 þ cells (lower panels). Interestingly, the CD4 and CD8 co-staining reveals a significant level (B12%) of an uncharacterized, double-negative (CD4 À CD8 À ) TCRb þ cell type in these tumors. The presence of these cells, together with both CD4 þ and CD8 þ single-positive T cells, indicates that these tumors do not arise from a single T-cell clone as would be the case in T-cell lymphoma. To verify the polyclonal origin of the T cells in these masses, a PCRbased approach 15 was used to detect rearrangements within the TCRb locus ( Figure 3b). Three primers sets spanning TCRb diversity (D) and junction (J) regions 1 and 2 were used to amplify fragments of the locus from tumor DNA. Controls included DNA representing the broad TCRb diversity of thymocyte DNA, the clonal TCRb rearrangement from a mouse iNKT cell line and the nonrecombined, germ line TCRb locus in mouse ES cell DNA (Figure 3b). The genomic DNA from 15 individual tumors was analyzed and all samples exhibited a polyclonal banding pattern similar to that of tumors 1, 2 and 3 presented in Figure 3b. These results confirm the polyclonal origin of the T cells that populate these lesions.
To investigate the mechanism underlying tumor formation in CD2-HA-BATF Tg mice, single-cell suspensions of tumor tissue were cultured in the presence of bromodeoxyuridine (BrdU) and DNA replication quantified by enzyme-linked immunosorbent assay (ELISA). Cells from the lymph nodes of WT littermates were used as controls. Previous studies had demonstrated that BATF expression inhibits the proliferation of T cells in vitro 5,14 and the results shown in Figure 3c confirm that Tg-tumor cells do not proliferate as well as WT controls. These data provide convincing evidence that the LPD detected in aged CD2-HA-BATF mice cannot be explained by an increase in the proliferative capacity of the BATFexpressing cells.
If the accumulation of T cells in these mice cannot be attributed to increased proliferation, perhaps it is due to an impaired apoptotic response that, over time, allows T cells that would normally be eliminated during the course of an adaptive immune response to persist and accumulate in lymphoid tissue. To address this hypothesis, purified CD4 þ T cells from the spleens of 8-12-week-old CD2-HA-BATF mice were exposed to an apoptotic stimulus in vitro and the death response of the culture was measured by the immunodetection of cleaved Pro-caspase 3. Initially, the response of the cells to extrinsic apoptotic signals was examined and the results demonstrated that activation-induced cell death was equivalent for Tg and WT T cells (data not shown). Subsequently, an established in vitro protocol of cytokine withdrawal 16 was used to compare the activated cell autonomous death (ACAD) 17 response of WT versus Tg T cells. In this case, under conditions of IL-2 withdrawal, the presence of Caspase 3 was detected readily in control WT cells within 24 h. In contrast, there was dramatically less Caspase 3 detected in lysates from Tg T cells, despite the fact that these cells appear to express more Pro-caspase 3 than their WT counterparts (Figure 3d). These data suggest that overexpression of BATF impacts the T-cell gene expression program in a manner that alters how T cells respond to intrinsic apoptotic signals.
Further support for apoptotic suppression by BATF was obtained using the terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay. CD4 þ cells were isolated and apoptosis in control and in IL-2 depleted cultures was quantified after 24 h by counting cells that stained positive for DNA strand breaks (Figure 4a). Assays were performed using WT cells, CD2-HA-BATF cells and cells isolated from Batf null mice. The cumulative data are presented in Figure 4b. Using the level of apoptosis measured for Tg cells following cytokine withdrawal as the baseline, WT cells expressing a normal level of Batf show a 2-fold increase in apoptosis and Batf null cells show a 4-fold increase. These data suggest a putative function for Batf as a molecular rheostat that determines the signal strength of apoptotic induction during ACAD.
The apoptotic response of T cells to trophic factor withdrawal relies on the differential expression of Bcl-2 family proteins. [17][18][19] Notably, to coordinate the mitochondrial events necessary to trigger Cytochrome c release and the subsequent activation of Caspases, transcriptional and posttranslational mechanisms are used to decrease the ratio of the anti-apoptotic protein, Bcl-2, to the pro-apoptotic protein, Bim. 20 To evaluate the impact of Batf on Bcl-2 and Bim transcripts, WT, Tg and Batf null CD4 þ T cells were isolated and gene expression changes captured in RNA prepared before, and 12 h following, IL-2 withdrawal. Semi-quantitative PCR and RT-qPCR were performed to detect and to quantify Bcl-2 and BimEL mRNA, respectively (Figure 4c). The RT-qPCR results (Figure 4c, right panel) are graphed relative to transcript levels in WT cells before IL-2 withdrawal which were set to 1 (data not shown). Following IL-2 withdrawal, the Tg cells do not fully downregulate Bcl-2 mRNA and, accordingly, retain a low level of BimEL mRNA. Conversely, while there is no statistical difference in the level of Bcl-2 mRNA in Batf null and WT cells, BimEL mRNA is 2-fold higher in Batf null cells than in WT cells. These observations favor a model in which Batf participates in nuclear events that promote an anti-apoptotic gene expression program and suppress the intrinsic, T-cell apoptotic response.

Discussion
This study describes a novel function for the AP-1 transcription factor, Batf, as a mediator of nuclear events contributing to the suppression of ACAD in CD4 þ T cells. We propose that, over time, the resistance to apoptotic signaling conferred on cells overexpressing Batf results in the accumulation of T cells in lymphoid tissues of CD2-HA-BATF Tg mice. The observation that the tumors arising in these mice are polyclonal is consistent with this model and defines the condition as T-cell LPD as opposed to T-cell lymphoma. The etiology of the vast majority of human LPDs remains unknown. 21 One exception is autoimmune lymphoproliferative syndrome (ALPS), 22 which is characterized by a defect in the extrinsic (i.e., death receptor mediated) apoptotic pathway. BATF drives formation of an LPD in mice by suppressing the T-cell response to intrinsic apoptotic signaling. This finding suggests an additional molecular mechanism that should be investigated in the ongoing effort to further classify human LPD.
As the name implies, ALPS presents as a polyclonal accumulation of T cells and is associated with high levels of autoantibody production. 22 In this regard, previous work with Batf null mice revealed an immunoglobulin-deficient phenotype produced as a consequence of combined defects in Th17 and Tfh-cell development and in the mechanism of Ig classswitch recombination. 10 It has been demonstrated that Batf null mice are resistant to experimental autoimmune encephalomyelitis (EAE) 7 and therefore, one might predict that EAE would be accelerated in mice that overexpress Batf. Although we have not examined this response in CD2-HA-BATF mice, we have compared the resting levels of immunoglobulin in sera from Tg mice and nontransgenic (WT) littermates. Results show that all of the class-switched Ig subclasses examined in these mice are increased 2-4 fold (Figure 5a). To investigate if self-reactive antibodies comprise a significant portion of this serum Ig, an ELISA for anti-nuclear antigen was performed. After normalizing for differences in total Ig content, more than a 2-fold increase in reactivity was detected in the sera of CD2-HA-BATF Tg mice (Figure 5b). These data correlate nicely with our observations in Batf DZ/DZ mice 10 and suggest that the Batf-induced alterations in T-cell homeostasis that lead to LPD also have a permissive effect on the development of autoimmune disease. Subjecting CD2-HA-BATF Tg mice to experimental models of immune-mediated disease will be used as a further test of this possibility.
The findings from the present study and other descriptions of elevated Batf expression in human and mouse peripheral lymphoid tumors 12,13 suggest that the influence of Batf on AP-1 activity and on the transcription of specific genes has a role. ACAD, the apoptotic process that is impaired in CD2-HA-BATF T cells, is associated with both transcriptional and post- translational events that increase the ratio of pro-apoptotic (e.g., Bim) to anti-apoptotic (e.g., Bcl-2) proteins and, by doing so, promote Cytochrome c release by mitochondria and the activation of cytosolic pro-caspases. 17,18,23,24 The shift from an anti-to a pro-apoptotic program in T cells occurs by a twostep process. The first step triggers Bcl-2 levels to decline as result of changes in the cytokine environment 25 or increases in reactive oxygen species (ROS). 26,27 Bcl-2 downregulation is not sufficient, however, and apoptosis requires a second step that is mediated by an increase in Bim expression. As prior studies demonstrate that BATF functions as an AP-1 inhibitor, 4,5 the influence of BATF overexpression on decreasing Bcl-2 levels is probably indirect. On the other hand, the reduced level of the BimEL transcript in CD2-HA-BATF Tg T cells and the dramatically elevated level of BimEL mRNA in Batf null cells are both consistent with a direct role for Batf as a negative regulator of Bim transcription. Interestingly, apoptosis following trophic factor withdrawal in neurons has been shown to rely on the induction of Bim transcription by a c-Jun containing AP-1 complex that binds directly to an AP-1 motif in the Bim promoter. 28,29 Furthermore, in hepatocytes, the generation of ROS triggers apoptosis by promoting the Erkdependent induction of c-Fos and increased Bim transcription through this same AP-1 site. 30 Batf is not expressed neurons or hepatocytes making it worthwhile to identify the components of the AP-1 complex that are responsible for upregulating BimEL mRNA in T cells and establishing how Batf may alter the activity of this AP-1 complex to inhibit the induction of Bim gene transcription during ACAD.
Our analysis of the Batf-associated apoptotic defect has focused on CD4 þ T cells largely because of the CD4 þ T-cell bias displayed by the tumors. However, this does not rule out that apoptotic pathways in peripheral CD8 þ T cells from CD2-HA-BATF and Batf null mice also are affected. In fact, while this work was in its final stages, Kuroda et al. 11 published a study in which WT and Batf null mice were used to establish a role for Batf in the later stages of CD8 þ effector T-cell differentiation and survival. In that study, Batf was shown to have a positive impact on cellular energy metabolism in CD8 þ T cells, largely by inhibiting expression of the histone deacetylase Sirt1 which, in turn, allowed for the expression of genes necessary for cell survival. At which stage the expression of Batf might affect Sirt1 and the proper epigenetic remodeling of loci critical to the function of CD4 þ T-cell subsets is an interesting question. Also of interest are studies to establish if the tumor cells in CD2-HA-BATF Tg mice reflect a T-memory phenotype, which might be predicted based on our results and those of Kuroda et al. 11 The mouse model described here provides novel opportunities to investigate how mis-regulation of the AP-1 transcription factor in T cells contributes to the improper control of apoptotic pathways and to the development of LPD. The high penetrance of this BATF-driven condition, together with the ability to detect the onset of these tumors using noninvasive MRI, should allow for testing both genetic strategies and targeted therapeutics for their effectiveness against T-cell LPD.

Materials and Methods
Mice. C57BL/6 CD2-HA-BATF and Batf DZ/DZ mice have been described previously 10,14 and were maintained in a specific-pathogen free animal facility according to institutional guidelines. CD2-HA-BATF mice overexpress the human BATF protein fused at the amino terminus to HA. The human and mouse BATF proteins differ by five amino acids and all are conservative changes located outside the core basic leucine zipper domain. Mice used in experiments were between 8 and 12 weeks of age unless noted otherwise.
Histology and IHC. Tissues were processed for hematoxylin and eosin (H&E) staining and IHC as described. 31 Sections were viewed using an Olympus BX41 microscope (Olympus Corp., Center Valley, PA, USA) and photographed using a Microimager II camera (Qimaging, Surrey, BC, Canada) and Northern Eclipse software (EMPIX Imaging Inc., Mississauga, ON, Canada). The comparison of spleen white pulp was made at Â 4 magnification using Image J software (NIH, Bethesda, MD, USA) to circumscribe the areas of white pulp area within the total area of spleen. White pulp area was expressed as a percentage of total spleen area and was averaged from the analysis of four, independent preparations per genotype. IHC was performed using the following primary antibodies: anti-CD79a (clone# HM57, Dako, Carpinteria, CA, USA), rabbit antihuman CD3 (Dako) and rat anti-HA (clone# 3F10, Roche, Indianapolis, IN, USA) and signal-detection reagents from Vector Laboratories (Burlingame, CA, USA). IHC images are Â 10 magnification, except for the inset which is Â 40. This IHC panel was repeated for four different tumors with similar results.
Clonality assay. Genomic DNA was isolated from 15 independent CD2-HA-BATF tumors, the thymus tissue of a C57BL/6 mouse, ES 129 cells and 431A11 iNKT cells 32 using phenol/chloroform extraction and the Wizard DNA Clean-UP System (Promega, Madison, WI, USA). The DNA samples were analyzed for D-J rearrangements in the TCRb locus using the primers and amplification conditions described by Kawamoto et al. 15 Cell proliferation assay. DNA synthesis was measured using a BrdU Cell Proliferation Assay Kit (Millipore, Billerica, MA, USA). Single-cell T-cell suspensions were prepared from CD2-HA-BATF lymph node tumors and agematched, WT lymph nodes using CD19 þ B-cell depletion (Miltenyi Biotech, Auburn, CA, USA). Cells were plated at 2 Â 10 4 cells/well in 96-well plates and stimulated with anti-CD3 and anti-CD28 antibodies as described. 8 After 48 h, BrdU was added for 24 h and labeled DNA quantified by ELISA.
Cytokine withdrawal experiments. Splenocytes were prepared and CD4 þ T cells isolated using a CD4 þ T-cell isolation kit (Miltenyi Biotec, Auburn, CA, USA). T-cell activation and cytokine withdrawal were performed as described. 16 Briefly, T cells were activated for 72 h in 100 U/ml IL-2 (R&D Systems, Minneapolis, MN, USA), 5 mg/ml soluble anti-CD28 and plate-bound anti-CD3e (BD Biosciences). Following 24 h of culture in IL-2 alone, the IL-2 was withdrawn. Samples for immunoblot and cytospin preparations were collected 24 h post-withdrawal. RNA samples were harvested 12 h post-withdrawal.
Immunoblots. To detect Batf protein, T cells were isolated from spleen, stimulated with anti-CD3 and anti-CD28 antibodies as described 8 and immunoblotted as described 33 using rabbit anti-BATF serum. 34 Reactivity with anti-b-actin antibody was used as the positive control. For Pro-caspase 3 and Capase 3, protein isolated from T cells that had been subjected to cytokine withdrawal was immunoblotted using rabbit anti-Caspase 3 antibody (Cell Signaling Technologies, Danvers, MA, USA). Detection of Hsp90 using rabbit anti-Hsp 90 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) was used as the positive control.

MRI.
A monthly, longitudinal MRI study was performed using 11 CD2-HA-BATF mice and 10 sex-matched, WT littermates beginning at 4 month of age and ending at 14 months of age. The animals were imaged while sedated with 70-90 mg/kg ketamine and 5-10 mg/kg xylazine administered intraperitoneally. Images were acquired using a 3.0 Tesla General Electric Signa HDx scanner and a transmit/ receive knee array coil (GE Healthcare, Piscataway, NJ, USA).
TUNEL assay. Splenocytes from 9 WT, 6 CD2-HA-BATF and 6 Batf DZ/DZ mice were enriched for CD4 þ T cells by Macs (Miltenyi Biotech) and subjected to cytokine withdrawal as described above. After 24 h, cells were transferred to slides using a CytoSpin 4 Cytocentrifuge (Thermo Fisher Scientific, Waltham, MA, USA). TUNEL of apoptosis-induced DNA damage was assessed using the In Situ Cell Death Detection Kit (Roche). TUNEL-positive cells were expressed as a percent of total cells (DAPI positive) per field at Â 20 magnification. Three fields per slide were counted and averaged to obtain the percent apoptotic T cells for each mouse spleen. Analysis of mRNA expression. Splenocytes were enriched for CD4 þ T cells and subjected to cytokine withdrawal as described above. After 12 h, RNA was isolated and converted to cDNA. SYBR green (Roche) RT-qPCR was performed and results analyzed as described. 10 Hprt and b-actin primers have been described previously 10 and primers for Bcl-2 and BimEL are from Kuribara et al. 35 Results were normalized to Hprt expression and DDCt values used to calculate the relative expression level of each mRNA. Semi-quantitative RT-PCR was performed as described 34 for 25 cycles using primers for b-actin, 34 Bcl-2 36 )and BimEL. 35 DNA products were visualized by ethidium bromide staining following resolution by 3% agarose gel electrophoresis.
ELISA. Blood from mice was collected by cardiac puncture and allowed to clot at room temperature in Microtainer Serum Separator Tubes (BD Biosciences). Serum was isolated by centrifugation. ELISA for the IgM, IgG1, IgG2C, IgA and IgE isotypes were perfomed as previously described. 10 Values were normalized and expressed as a fold-over the WT control, which was set to 1. ELISA for anti-nuclear antibodies (ANA) was performed using the Mouse ANA Ig ELISA Kit (Alpha Diagnostics, San Antonio, TX, USA). In this case, total protein content was normalized by adding Ig to the non-Tg sera before assay. Values in units/ml were calculated from a standard curve using the reagents provided in the kit.