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Effects of Lactobacillus casei Shirota on immune function

Methods: PBMC were obtained from 5–19 healthy adults. LcS were grown anaerobically in MRS broth and harvested in the exponential phase. PBMC (1 · 10 cells/ml) were exposed to three different concentrations of viable LcS for 24 h with or without 2.5mg/ml ConA. The activation markers, CD69 and CD25, on T cell subsets were assessed by flow cytometry. For measurement of cytokine production, PBMC were stimulated for 24 h by three different concentrations of viable LcS with or without 1mg/ml LPS and cytokines were measured by ELISA. Natural killer (NK) cell activity stimulated by LcS at 10 CFU/ml was assessed by flow cytometry, using K562 cells as target cells. Monocytes were depleted from PBMC by magnetic beads. PBMC and monocyte-depleted PBMC were compared for their response to LcS-induced activation and cytokine production.