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Performance of the indirect antiglobulin test does not show a circadian rhythm

Performance of the indirect antiglobulin test by workers in a blood bank was examined for signs of a rhythmical relationship between the time of day and the frequency of error. All participants were experienced in blood group serology and completed sets of 12 antiglobulin tests at 4‐hour intervals over a 24‐hour period. Ten of the original 17 participants repeated the experiment after an interval of 7 months. About 50 percent of participants made more errors in the daytime or the nighttime in each experiment; however, these groups were unstable and contained different individuals in each experiment. Significant (p less than or equal to 0.05) time‐related cosinor rhythms could be demonstrated for four individuals in one experiment, but this finding was not reproduced in a second experiment. There was no indication that the amount of sleep deprivation which occurred had a significant influence on the investigation, and the individual frequencies of error at 0800 hours appeared to be a stable individual characteristic. The results highlight the importance of examining workplace‐related performance as an individual rather than a group phenomenon.