{'fraction_non_alphanumeric': 0.02746268656716418, 'fraction_numerical': 0.016119402985074627, 'mean_word_length': 4.901408450704225, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Transporting Vitrified Murine Oocytes Reduces Their Overall Survival on Thawing [158]

INTRODUCTION: Vitrification is the standard of care for freezing oocytes and survival rates of thawed oocytes have increased compared with the older method of slow freezing. The objective is to identify if by forming a glassy state induced with vitrification, are the oocytes at greater risk for microarray fracturing with shipment? MATERIALS AND METHOD: Eighty mouse eggs were commercially obtained, thawed, vitrified, then divided into two groups where group 1 (study) was placed in a vapor nitrogen shipper and carried around in a vehicle for 1 week. Group 2 (control) was placed in a separate shipper but was never moved from the laboratory. All oocytes were thawed and then examined for viability. To reduce costs, reused CryoLeafs were used in each group with placement of six to seven oocytes on each straw, which increased the time necessary to complete freezing and thawing. RESULTS: Of the 80 mouse oocytes, 73 survived the initial thaw and then were refrozen. In group 1 (study), 10 of 36 (27%) survived the second thaw and in group 2 (control), 20 of 37 (54%) survived the second thaw. These differences were significant by &khgr;2 analysis with P<.05. CONCLUSION: The transport of vitrified mouse oocytes lowers overall survival on thawing and the oocytes may be more susceptible to damage from transport because of the induced glass-like state. The low percentage of eggs surviving in each group was the result of many factors including the fact that increased number of oocytes on each Cryoleaf required extra time to freeze and thaw, which led to the overall low survival.