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Colorimetric determination of physostigmine in pharmaceuticals.

A colorimetric method for determination of physostigmine is suggested based on measurement of the yellow color produced when physostigmine dithiocarbamate reacts with copper. The color obeys Beer's law in the range of 65–400 mcg. The reaction was found to be specific for physostigmine in the presence of other alkaloids and nitrogen-containing substances, using a pH 8.6 buffer and sodium bicarbonate.