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BSA amplified ROS generation from Anthrarufin derived Carbon dot and concomitant Nanoassembly for combination antibiotic-photodynamic Therapy application.

Amplification of Reactive Oxygen Species (ROS) generation through covalent conjugation of Bovine Serum Albumin (BSA) with newly synthesized, ROS producing Carbon Dot (CD) upon visible light irradiation is reported for the first time. Derivatization of surface carboxyl functional groups of Anthrarufin derived green emitting CD with amine functionality of BSA usher distinct changes in photophysics of CD including an unprecedented ~50 nm shift in its excitation maxima, decrease in fluorescence lifetime and concomitant increase in ROS generation. Substantial conformational changes of BSA was witnessed upon conjugation with CD, rendering the BSA-CD conjugate resistant to pepsinolysis. A protease-proof nano-assembly was derived from the BSA-CD conjugate through desolvation that simultaneously hosts a prototype antibiotic and generates ROS with excellent efficiency, making it an attractive platform for antibacterial Photodynamic Therapy (A-PDT) applications. Systemic annihilation of both gram-positive and gram-negative bacteria was achieved with the BSA-CD nano-assembly and envisioned as alternatives to traditional photosensitizers.