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In Vivo Biosynthesis of 35S‐Substance P from [35S]Methionine in the Rat Striatum and Its Transport to the Substantia Nigra

Abstract: Rats with a push‐pull cannula implanted in the right striatum were used to study the biosynthesis of 35S‐substance P (SP) from [35S]methionine and its transport to the ipsilateral substantia nigra. [35S]Methionine was delivered for 2, 3 or 5 h to the push‐pull cannula. 35S‐SP in striatal and nigral tissues was estimated after immunoadsorption and HPLC. Higher levels of 35‐SP in striatal homogenates were found after a 5‐h labelling period. 35S‐SP biosynthesis was inhibited when cycloheximide was superfused together with [35S]methionine. The identity of 35S‐SP was further checked by its conversion into 35S‐SP sulphoxide. After a 5‐h labelling period, 35S‐SP was also recovered in the substantia nigra. This was not the case after hemisection of striato‐nigral fibers. When rats were killed 15 or 24 h after the 5‐h labelling period, 35S‐SP levels in the substantia nigra were higher than those found just after the 5‐h labelling period, while the reverse was observed in the striatum.