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FORMING OF 3D NEURONAL PATHWAY BY NEURONAL BLOCK ASSEMBLY

This paper describes a method of three-dimensional (3D) neuronal pathway formation in vitro by bottom-up neuronal tissue block assembly (Fig.1). We first formed the neuronal tissue blocks by molding uniform-sized neurospheroids into the PDMS mold chambers. We then assembled a millimeter-sized cortex-hippocampal tissue by connecting cortical and hippocampal tissue blocks. We found that cortical and hippocampal neurons extended their axons to each other, and that 3D neuronal pathway was successfully formed in the 3D environment. We believe that our neuronal block assembly enables the fabrication of in vivo-like neuronal pathway, and that this system is useful tool for neuronal tissue engineering, pharmacological assay for the screening of drugs and toxins to neuronal pathway.