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Induction of c-fos expression by tributyltin in PC12 cells: involvement of intracellular Ca(2+).

The effects of tributyltin on the expression of immediate early genes, c-fos and c-jun, were examined in PC12 cells using reverse transcription polymerase chain reaction analysis. Tributyltin at concentrations of more than 0.4 μM induced the expression of c-fos after 15 min exposure. The induction of c-fos was accompanied with c-jun expression. Tributyltin-induced c-fos expression was abolished completely by actinomycin D, indicating it was due to transcriptional activation of the gene. Chelation of intracellular Ca(2+) suppressed the expression of c-fos markedly, while removal of external Ca(2+) did not. These results suggest that Ca(2+) mobilized from intracellular stores played a major role in the tributyltin-induced transcriptional activation of c-fos in PC12 cells.