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Biosynthesis of -cyanoalanine in Vicia sativa.

Administration of cysteine-U-14C to Vicia sativa at the time of seed maturation resulted in extensive labeling of β-cyanoalanine. As extensive incorporation also occurred when cysteine-U-14C was given to the pods through the cut ends of their stalks, it is suggested that the synthesis of β-cyanoalanine takes place in this tissue. The effect of cyanide on the incorporation of serine-U-14C into glutamyl-β-cyanoalanine in Vicia sativa seedlings was also investigated. It was found that extensive incorporation of radioactivity occurred only when cyanide was given to the seedlings along with radioactive serine. Incorporation of radioactivity was much less when cyanide was added to the medium 8 h after the administration of serine-U-14C.