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GLC determination of methadone in human urine.

Human urine is buffered with an equal volume of pH 9.3 borate buffer and extracted with a chloroform-isopropanol (3:1) solution. After the extract is concentrated to dryness, the resultant residue is dissolved in methanol containing 4-benzylbiphenyl as an internal standard. The solution is assayed for methadone content by GLC. This method was evaluated for concentrations of methadone ranging from 3 to 30 mcg./ml. in human urine and gave an overall precision and accuracy of ± 7% (RSD and RE).