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Nanomaterials‐assisted metabolic analysis toward in vitro diagnostics

In vitro diagnostics (IVD) has played an indispensable role in healthcare system by providing necessary information to indicate disease condition and guide therapeutic decision. Metabolic analysis can be the primary choice to facilitate the IVD since it characterizes the downstream metabolites and offers real‐time feedback of the human body. Nanomaterials with well‐designed composition and nanostructure have been developed for the construction of high‐performance detection platforms toward metabolic analysis. Herein, we summarize the recent progress of nanomaterials‐assisted metabolic analysis and the related applications in IVD. We first introduce the important role that nanomaterials play in metabolic analysis when coupled with different detection platforms, including electrochemical sensors, optical spectrometry, and mass spectrometry. We further highlight the nanomaterials‐assisted metabolic analysis toward IVD applications, from the perspectives of both the targeted biomarker quantitation and untargeted fingerprint extraction. This review provides fundamental insights into the function of nanomaterials in metabolic analysis, thus facilitating the design of next‐generation diagnostic devices in clinical practice.