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Extraction of β-Carotene from Spirulina Using Ethanol and Acetone-Ammonium Sulphate Aqueous Two-Phase System

In this study,an aqueous two-phase system composed of 15%( W / W) ethanol and acetone( v / v = 1 ∶ 2)-24%( W / W) ammonium sulphate was selected to extract β-carotene from Spirulina. The effect of crude extract concentration,p H,extraction temperature on the extraction efficiency of β-carotene were investigated by single factor and BoxBehnken design methods. The results showed that the optimal conditions for the extraction of β-carotene were: crude extract concentration of 6%( W / W),p H of 8. 0 and extraction temperature of 30 ℃. The maximal extraction efficiency( 94. 55%) was obtained under the optimal conditions. These results suggested that ATPE is efficient in extracting β-carotene and has the potential to be used in natural β-carotene extraction industry.