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Pancreatic insulin and glucagon content and secretion after infection of mice with EMC virus [proceedings].

experiments. Fig. 2 shows the rate of entry over the first 3min in the presence and the absence of insulin. With starved cells, unlike the previous experiments, the insulin effect was greater at 3 min than at 1 min. We have shown that the uptake of 3-0-methylglucose by ‘facilitated diffusion’ into suitably cultured human lymphocytes is linear for 3min at 4°C under the conditions described. The initial rate of entry can be enhanced by preincubation of the cells in the presence of insulin. This effect, optimized by selection of culture conditions, media and handling procedures, could become the basis of a simple and convenient bioassay for insulin in vitro.