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[Study on preparation of gypenosides liposomes composed of sphingomyelin and cholesterol and its quality evaluation].

OBJECTIVE
To discuss the feasibility of preparing gypenosides liposomes with sphingomyelin and cholesterol, and optimize the preparation process and prescription.


METHOD
Gypenosides liposomes were prepared with sphingomyelin and cholesterol. The entrapment efficiency was determined by the protamine precipitation method. The entrapment efficiency was taken as the evaluation index to screen out the optimum preparation process for the new-type gypenosides liposomes. The preparation process was optimized by the orthogonal design. The new-type gypenosides liposomes were characterized by grain size, potential and atomic force microscope (AFM).


RESULT
Ethanol injection was the optimum process to prepare gypenosides liposome with sphingomyelin and cholesterol as follow: the ratio of gypenosides to sphingomyelin was 1: 10, the ratio of sphingomyelin to cholesterol was 4: 1, the pH of PBS buffer solution was 7.0, the hydration temperature was 45 degrees C, the entrapment efficiency was 79.06%, particle size was 191.4 nm, the Zeta potential was -33.16 mV, and the morphology were round under AFM.


CONCLUSION
It was feasible to prepare gypenosides liposome with sphingomyelin and cholesterol. The gypenosides liposomes prepared by the optimum preparation process were good in morphology, particle size and reproducibility.