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[Metallothionein isoforms gene expression induced by cadmium in human peripheral blood lymphocytes].

OBJECTIVE
To explore gene expression of metallothionein 1 (MT-1) isoforms in human peripheral blood lymphocytes.


METHODS
mRNA of expression representing the seven active subtypes of MT-1 gene was determined in cultured human peripheral blood lymphocytes (HPBLs) before and after exposure to cadmium by quantitative RT-PCR.


RESULTS
Basal expression of MT-1X, MT-1A in HPBLs was similar to expression of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase. In contrast, the basal gene expression of MT-1H, MT-1F, MT-1E, MT-1G was a little transcript in HPBLs. No signal was detected for the MT-1B. There was a sex difference (P < 0.05) in basal gene expression of MT-1E. The level of gene expression of MT-1A, MT-1E, MT-1F, MT-1G, MT-1H and MT-1X was significantly increased (P < 0.05), but not the level of MT-1B after exposure to cadmium.


CONCLUSION
It was suggested that gene expression of MT-1F, MT-1G, MT-1H and 1X in HPBLs could be used as potential exposure biomarker.