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Isolation and characterization of efficient poly - -hydroxybutyrate (PHB) synthesizing bacteria from agricultural and industrial land

A B S T R A C T Halophiles are extremophile organisms that thrive in environments with very high concentrations of salt. In the present study, halotolerant and halophilic bacterial strains were isolated from industrial and agricultural land. These strains were grown in media with 2 molar NaCl concentrations. The growth of these strains was observed on three different growth media (nutrient agar, trypticase agar and luria agar), best growth was observed on luria agar media. Growth on selective media (DSC­97) was also observed. After optimization of different culture conditions, isolated strain was characterized biochemically.Bacterial isolates were also characterized on the basis of Gram s staining, sudan black and Simple staining etc using microscope. The most producer isolate was identified to the molecular level using 16S rRNA as Bacillus subtilis and Pseudomonas sp.