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trans‐10, cis‐12‐Conjugated Linoleic Acid Reduced omega‐3 Fatty Acid in Liver and Heart Tissue Of Mice

This study was designed to examine the incorporation of conjugated linoleic acid (CLA) isomers into lipids of various tissues (liver, adipose tissue, spleen and heart), and the effect of these diets on tissue lipid weights and fatty acid composition. Eight week old, female mice (n=6/group) were fed either a control diet, or diets supplemented with 0.5% of either c9,t11‐ or t10,c12‐CLA isomers for eight weeks. Feeding t10, c12‐CLA, led to a 400% increase in the liver lipid weight, a 75% reduction in adipose tissue lipids, but no change in the lipid weights of spleen or heart. Feeding of c9, t11‐CLA did not alter the lipid weights of tissues examined. Feeding of t10, c12‐CLA increased the wt% concentration of 18:1n‐9 by 20–50% in liver and heart compared to animals fed the control diet; c9, t11‐CLA decreased 18:1n‐9 in liver, adipose, and spleen but not in heart. C9, t11‐CLA increased the wt% concentration of 18:2n‐6 by 20 % in liver and adipose tissue lipids; t10, c12‐CLA decreased 18:2n‐6 by 50% in liver. In the t10,c12‐CLA group, the wt % of docosahexaenoic acid (DHA) and the ratio between n‐3 and n‐6 polyunsaturated fatty acid decreased by 25% in heart, while DHA increased by >700% and 20:4n‐6 decreased by >95% in the spleen lipids. In contrast, in the c9, t11‐CLA group, 20:4n6 increased by 67% in lipids of the spleen, with no changes in the heart. The potential consequences of such changes in heart and spleen fatty acid composition on immune and cardiovascular health need to be investigated.