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Effects Of Dietary Fiber And Exercise On Cognition, Muscle Function, And Scfa In Young Mice: 1896 Board #48 June 2, 3: 30 PM - 5: 00 PM.

PURPOSE: Short chain fatty acids (SCFAs) contribute to immune and brain function and are produced by gut bacteria through metabolism of fermentable fiber. METHODS: Young (6 week) male C57Bl/6J mice were fed AIN-93M diet or AIN-93M with 5% pectin substituted for cellulose for 6 weeks and voluntarily exercised on a wheel (VWR) or remained sedentary (SED), making up four groups with n=5/group. After 4 weeks, mice underwent muscle and cognitive function testing. At sacrifice, cecal contents were collected for quantitation of SCFAs. RESULTS: VWR mice maintained body weight, while Sed mice gained 2 grams (p<0.001, time*VWR). Neither exercise (p=0.233) or pectin (p=0.481) affected performance on the active avoidance task. In the Morris Water Maze task, neither exercise (p=0.908) or pectin (0.781) affected latency to find the hidden platform during the acquisition phase, and no significant differences were found during the probe trials for either treatment. Interestingly, both pectin and exercise decreased grip strength, although only the pectin effect remained after normalization to body weight (p=0.004). As expected, VWR increased performance on the rotarod (p=0.028) and exhaustive fatigue test (p=0.012). Pectin increased cecal acetate (p=0.001), propionate (p=0.002), and butyrate (p=0.005). Interestingly, there was also a significant VWR effect for increased acetate (p=0.030) and a significant VWR effect (p=0.002) and interaction (p=0.031) such that the pectin + VWR group had the highest levels of cecal propionate. CONCLUSION: In conclusion, pectin and/or VWR had little effect on cognitive function but tended to increase cecal SCFAs in young mice, which may have implications for brain and immune system function. Funding from the Abbott-UIUC Center for Nutrition, Learning, and Memory,