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Failure of Gamma‐Interferon Induction in Germfree Mouse Spleen Cells by OK‐432, a Preparation of Streptococcus pyogenes

(5). It is produced in vitro upon exposure of human or mouse lymphoid cells to mitogens and viral or non viral antigen (2, 5). A previous study showed that immune interferon produced in vitro can be used as a quantitative indicator of T-cell-mediated immunity (5). A streptococcal preparation (OK-432) which has been shown to have immune modulating activity in experimental animals and humans induced interferon in the spleen cell cultures of various inbred strains of mice (10). OK-432-induced interferon was mainly IFNƒÁ, but also contained IFNƒ¿ and IFNƒÀ (10). Germfree animals play important roles in investigations that would otherwise become complicated by the presence of microbial flora. Since bacteria and bac terialproducts are known to induce the formation of IFN or enhance IFN produc tion,the germfree animal can be utilized as a more refined test animal for inves tigationsinvolving the production of IFN in response to bacterial stimuli (4). In this study, the capacity of spleen cells obtained from germfree, specific pathogen free (SPF) and conventional mice to produce IFN in vitro in response to OK-432 was investigated. The mice used in this study were germfree male ICR mice and their correspond ingSPF and conventional mice, which were originally obtained from the CLEA