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Preparation of giant lipobeads using a gel-assisted swelling method

Lipobeads are spherical vesicles of lipid membrane supported by a hydrogel core that hold promise as next-generation biosensors. However, previous methods for generating lipobeads had limitations due to the use of organic solvents and low ionic strength solutions. To overcome these limitations, we developed a method for forming lipobeads by using a gel-assisted swelling approach. Our results show that lipobeads form in osmotic strength solution and supposed to contain no residual organic solvent.