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The nucleotide composition of labelled RNA from spinal ganglia of chick embryo and the action of a specific growth factor (NGF).

Percent nucleotide composition has been determinated after ion-exchange chromatography of hydrolysates of RNA extracted from ganglia incubated in vitro with 32P for 3 or 5 hr, in the presence as well as in the absence of a specific growth factor (NGF). Analyses were carried out on both total RNA and high-molecular RNA separated by chromatography on MAK-columns. The main component of labelled RNA appears to be a high-molecular RNA with a nucleotide composition of DNA-like type, whereas R-RNA and its precursor represent only a minor amount. No significant difference has been detected between RNA from control and NGF-treated ganglia.