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[Studies and application of fluorescence of metacycline enhanced by synergetic effect of beta-cyclodextrin and DBS].

The fluorescence of metacycline enhanced by the synergetic effect of beta-cyclodextrin with anion surfactant sodium dodecylbenzene sulfonate (DBS) was studied. It was found that the fluorescence intensity of metacycline was greatly enhanced in the system of beta-CD-DBS. Based on this a new sensitive method for the determination of metacycline was established. The experiments indicated that MTC-beta-CD-DBS can form 1 : 1 : 1 ternary inclusion complex with apparent stability constant of 1 294 L2 x mol(-2), respectively. The fluorescence intensity was linearly related to the metacycline concentration in the range of 1.7 x 10(-7) to 8.8 x 10(-5) mol x L(-1) with a correlation coefficient of 0.996 6. The detection limit was 7.4 x 10(-9) mol x L(-1) this method was satisfactorily applied to the determination of metacycline in pharmaceutical preparations. The recovery was within the range of 95% to 106%.