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MALDI-TOF Mass Spectral Characterization of Polymers Containing an Azide Group: Evidence of Metastable Ions.

Introduction The copper catalyzed Huisgen 1,3-dipolar cycloaddition ”click” reaction,1 has become an invaluable coupling reaction due to its uncommon combination of extremely high coupling yields and broad functional group tolerance. This versatility has been demonstrated in numerous applications2 including the labeling of biological molecules (DNA, proteins, cell surfaces, etc.) both in vitro and in vivo,3,4 synthesis of biomedical materials,5 the crosslinking self assembled nanostructures,6 and the functionalization of two-dimensional surfaces.7