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Detection of interleukin‐8 on microgapped dual electrodes for measuring asthma complication

Detection of asthma by a suitable biomarker is mandatory for the early identification, which helps in providing a right medication for the complete cure. Interleukins (ILs) have played a major role in asthma; in particular IL‐8 is highly correlated with severe asthma. This research was focused on to detect IL‐8 level by its partner antibody on a microgapped dual electrodes sensor. The sensing surface was modified into graphene oxide (GO), and an antibody was fixed by using the amine‐aldehyde linker. GO enhanced the antibody immobilization and the consequence electric current flow upon interacting with IL‐8. The detection limit of IL‐8 was reached to 10 pg/mL in a linear range from 1 to 10,000 pg/mL with the regression of y = 0.7246x − 0.906 (R² = 0.9758); further, the sensitivity falls at 1 pg/mL. The surface does not show the antifouling effect with control antibody, and proteins, indicating the specific IL‐8 detection. The detection of IL‐8 helps in diagnosing and solving the related problems of asthmatic patients.