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Thyroid Transcription Factor–1 Immunocytochemical Staining of Pleural Fluid Cytocentrifuge Preparations for Detection of Small Cell Lung Carcinoma

OBJECTIVE
To assess whether thyroid transcription factor-1 (TTF-1) immunostaining of cytocentrifuge slides previously stained by the Papanicolaou technique is feasible and useful in diagnosing small cell lung carcinoma (SCLC) in pleural fluid.


STUDY DESIGN
Eleven Papanicoalou-stained cytocentrifuge preparations of pleural fluid from 11 patients with SCLC were reviewed. The initial cytologic diagnoses were 5 positive, 1 suspicious and 5 negativefor SCLC. After transferring the cytologic material to a positively charged slide, immunostaining was performed using mouse monoclonal antibody against TTF-1.


RESULTS
The neoplastic cellularity varied among the single suspicious and 5 positive cases. The case with a suspicious diagnosis had scant tumor cells. All 6 cases showed nuclear stainingfor TTF-1 in most of the tumor cells. The other 5 cases with a negative cytologic diagnosis were negativefor TTF-1.


CONCLUSION
The results of this study indicate that immunostaining for TTF-1 can be successfully applied to previously Pap-stained cytocentrifuge preparations to assist in the diagnosis of SCLC in pleural fluid.