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Genetic characterization of human-derived hydatid cysts of Echinococcus granulosus in Lorestan Province, Western Iran.

The aim of the present investigation was genotyping of hydatid cysts of cystic echinococcosis (CE) patients residing in Lorestan province by sequencing and analyzing of the partial mitochondrial cytochrome c oxidase subunit 1 (cox1). A total of 26 hydatid cysts (6 hydatid cysts) from 6 patients suffering from hepatic CE and 20 formalin-fixed paraffinembedded (FFPE) tissue samples from 20 human patients with histologically confirmed CE. DNA was extracted, and genotyping was performed by sequencing and analyzing mitochondrial cox1 gene. All the samples were analyzed using mitochondrial cox1 primers. In sequencing analysis, the alignment of the determined sequence with those of knows genotypes of E. granulosus demonstrated that the isolates belonged to genotype G1 (sheep strain). Representative nucleotide sequence obtained in this study was deposited in the GenBank database under the accession number LC068913.1. The phylogenetic analysis showed two clusters. One of the clusters includes G3 genotype (HM5630221) and the other cluster represents all strains related to G1 and G3 genotypes which contain genotype defined in this study as well. The obtained findings demonstrated that G1 genotype is predominant strain of human CE in Lorestan province, Iran, which indicating the sheep-doge cycle in this area. Further studies with more sample size should be conducted to be sure of the sheep strain G1 which is predominant strain in this province.