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Protein phosphorylation in mitochondria – A study on fermentative and respiratory growth of Saccharomyces cerevisiae

Phosphorylation as a posttranslational protein modification is a common subject of proteomic studies, but phosphorylation in mitochondria is still poorly investigated. The study presented here applied 2‐DE to characterize phosphorylation in the yeast mitochondrial proteome and identified 59 spots corresponding to 34 phosphorylated mitochondrial or mitochondria‐associated proteins. Most of these proteins presented putative substrates of mitogen‐activated protein and target of rapamycin kinases, cAMP‐dependent protein kinase, cyclin‐dependent kinases and Snf1p suggesting them as key players in the phosphorylation of mitochondrial or mitochondria‐associated proteins. The dynamic behaviour of the phosphoproteome under a major metabolic change, the shift from fermentation to respiration (diauxic shift), was further studied. Eight proteins (Ald4p, Eft1p/2p, Eno1p, Eno2p, Om14p, Pda1p, Qcr2p, Sdh1p) had growth dependent changes in their phosphorylation, indicating a role of phosphorylation‐dependent regulation of translation, metabolic pathways (e.g. glucose fermentation, tricarboxylic acid cycle, pyruvate dehydrogenase and its bypass) and respiratory chain.