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Development of luminescent bacteria as tracers for geological reservoir characterization

This research project resulted from recognizing the problem of being unable to accurately distinguish communication between wells in producing oil zones which may or may not be continuous. Such a determination is necessary when considering Enhanced Oil Recovery (EOR) whether it is water flooding, carbon dioxide, or other methods which increase the sweep efficiency. Various kinds of chemical tracers are available, but they are expensive and many might be considered hazardous for underground aquifers. Other biological tracers are available, but have never been developed for oil reservoir conditions. Bioluminescent bacteria seemed an obvious candidate because they thrive in saline waters (usually 3% salt) which have been contaminated by oil spills.