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Parallel measurement of suspension array by freezing imaging of microspheres

In this paper, a 2D parallel measurement technology for suspension array was presented. Suspension array technology was a new type of biochip, in which microspheres were used as the carrier of bio-probes. It was usually detected by flow cytometry serially. To measure it in parallel, microchannels were used as analyzing platform. Microspheres flowing in the 2D microchannel were freezingly imaged by pulsed Xenon lamp and a microscopy objective in parallel. The image was captured with CCD. The microfluidic channel was designed and fabricated, which was a rectangle microchannel of 1mm x5Oum in cross-section. System performance design was derived. After the selection of CCD, relationship between the limitation of detection and the power of pulsed Xenon lamp was given. System parameter was provided. Some photography of experimental result was presented. Area measurement of suspension array in microchannel was realized. Compared with flow cytometry, this technology increased analyzing rate greatly, which could be thousands of microspheres per second.