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Cloning of Coding Sequence of Non-specific Transfer Protein from Polygonum sibiricum Laxm.and Expression under Salinity Stress

The full-length nsLTP, a non-specific lipid transfer protein gene, was cloned using RACE (rapid amplification of cDNA end) technology based on a nsLTP partial sequence obtained from a random clone in SSH library of Polygonum sibiricum Laxm. The acquired 604 bp sequence includes a 5′ untranslated region of 65 bp, a 3′ untranslated region of 227 bp with poly (A), and an open reading frame (ORF) encoding 140 amino acids. The sequencing result revealed that the gene, we termed as PsnsLTPs, contained a N-terminal signal peptide that was a typical conservative region of the nsLTP family. The expression analyses by RT-PCR showed that PsnsLTPs was expressed in leaves, stems and rhizomes of Polygonum sibiricum Laxm. Under the induction of 3% NaHCO_3, the expression of PsnsLTPs was significantly increased, which suggested that PsnsLTPs might play an important role in saline stress resistance.