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Intraglomerular expressions of IL‐1α and platelet‐derived growth factor (PDGF‐B) mRNA in experimental immune complex‐mediated glomerulonephritis

Both PDGF and IL‐I play important roles as autocrine growth factors for cultured mesangial cells, and may be closely associated with the progression of glomerulonephritis. In this study we investigated intraglomerular expressions of PDGF‐B and IL‐1α mRNA in mice with bovine serum albumin (BSA) nephritis, a model of immune complex‐mediated glomerulonephritis using the reverse transcription‐polymerase chain reaction (RT‐PCR) method. We also quantified intraglomerular PDGF‐B mRNA by the competitive PCR and studied the correlation between the level of intraglomerular PDGF‐B mRNA expression and the degree of observed glomerular injury. While expression of neither PDGF‐B nor IL‐lα mRNA was detected in glomeruli from control mice, both were strongly expressed in glomeruli from mice with BSA nephritis. IL‐1α mRNA in glomeruli showed low accumulation in mice with mild glomerular injury, and was increased in mice with moderate glomerular injury. In contrast, high intraglomerular expression of PDGF‐B mRNA occurred in all mice with mild glomerular injury and continued throughout the course of the disease. We observed no correlation between the level of PDGF‐B mRNA expression and the histologic grade of renal damage. These results suggest that PDGF and IL‐1 have different growth properties, and PDGF might play a role as a competence factor rather than a progression factor in the pathogenesis of immune complex‐mediated glomerulonephritis.