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Effects of ischemic preconditioning on apoptosis of rabbit atrioventricular node cells in vivo

AIM To study the effects of ischemic preconditioning(IP) on apoptosis,Bcl-2 and Bax expressions of atrioventricular node(AVN) cells in vivo in ischemic/reperfused rabbits.METHODS The 40 rabbit were divided into 5 groups randomly in this experiment: sham operate group,I/R A group,I/R B group,IP A group and IP B group.IP and I/R models of AVN cells ischemicreperfused were established in rabbits by obstructing the right coronary artery. The AST,LDH and CK expressions of blood serum were observed.The ultramicrostructure of AVN cells were observed by perspective electron microscope.Apoptosis AVN cells were detected with TUNEL and Bcl-2 and Bax expressions were detected with immunohistochemistry.RESULTS The expression of apoptotic AVN cells significantly decreased in IP group compared with that in I/R group(P0.05).Bcl-2 expression significantly increased in IP group compared with that in I/R group(P0.05) while Bax expression significantly decreased in IP group compared with that in I/R group(P0.05).CONCLUSION I/R induces and IP reduces AVN cells apoptosis in rabbits.IP increases the Bcl2 expression and decreases the Bax expression of AVN cells in ischemic/reperfused in rabbits.