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Lymphokine‐induced secretion of plasminogen activator by murine macrophages

Thioglycollate‐stimulated macrophages are known to release a plasminogen activator (PA) into the medium. In this study it was investigated whether macrophages could be activated to release PA after exposure to lymphokines. Macrophage monolayers obtained by 24 h culture of proteose peptone‐elicited murine exudate cells were incubated with lymphocyte culture supernatants. After 48 h the supernatants were replaced by serum‐free medium and the macrophages were incubated for another 24–48 h. These supernatants were assayed for PA as measured by the lysis of 125I‐labeled fibrin. The following results were obtained: