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The Effect Of The Muscle Biopsy Procedure On Blood Flow And Tissue Oxygenation: 1153 Board #7 May 28, 8

The percutaneous skeletal muscle biopsy procedure is a common method used in the study of skeletal muscle physiology. This procedure has been shown to disrupt glycogenesis, possibly due changes in blood flow and thus nutrient delivery. PURPOSE: To determine the effects of the percutaneous skeletal muscle biopsy procedure on blood flow and tissue oxygen saturation. METHODS: Twelve recreationally active males (age: 25 ± 5 years; height: 178 ± 6 cm; weight: 86.8 ± 12.5 kg; body fat: 13.6 ± 6.6%) rested in a supine position for 30 minutes before blood flow measurements were obtained from the common femoral artery of each leg using an ultrasound system (Terason t3000, Burlington, MA). The location for this measurement was approximately 2-3 cm proximal to the artery’s bifurcation. Tissue oxygenation was collected from a skin surface site midway between the greater trochanter and proximal border of the patella over the vastus lateralis, using near infrared spectroscopy (Inspectra StO2 Monitor; Hutchinson, MN). Following the initial blood flow and tissue oxygenation measurements, a biopsy of the vastus lateralis was conducted using a 5 mm Bergstrom biopsy needle with the aid of suction followed by additional blood flow and tissue oxygenation measures. RESULTS: There was no effect of biopsy on femoral artery diameter (pre, 8.35 ± 0.45; post, 8.19 ± 0.42 mm; p = 0 .412), blood velocity (pre, 6.48 ± 0.66; post, 6.10 ± 0.66 cm · sec ; p = 0.507), or flow volume (pre, 377 ± 62; post, 350 ± 64 ml · min; p = 0.415). Tissue oxygen saturation decreased from pre to post biopsy (pre, 69 ± 8 %: post, 61 ± 7 %; p = 0.0185). CONCLUSION: These data indicate that the skeletal muscle biopsy procedure does not impact blood flow, but does have an impact on tissue oxygen saturation. This physiological effect should be considered in studies using the percutaneous muscle biopsy procedure.