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Comparison Of The Appearance Of Cultured Cells Observed Using Scanning Acoustomicroscopy With That Obtained By Interference And Fluorescence Microscopy.

In XTH-2 cells (line derived from Xenopus laevis tadpole hearts) grown on glass, cyto-plasmic factors influencing acoustic impedance and attenuation were investigated using ultrasound of 1.6 GHz frequency. An overall correlation between the distribution of F-actin and zones with increased impedance is qualitatively assessed. Comparison of scanning acousto-microscope (SAM) images with those taken with a Mach-Zehnder type microinterferometer reveal a linear relationship between the amount of cytoplasmic mass penetrated by the sound waves, and sound attenuation. However, the attenuation coefficient for the cell periphery is considerably higher then that for the cell centre.