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1, 25‐dihydroxyvitamin D3 modulates lipid metabolism in 3T3‐L1 and HepG2 cell lines

Exogenous fatty acids up regulate the expressions of various genes involved in energy regulatory pathway. Fatty acids increases cellular glucose uptake and lipogenesis and reduce lipolysis, leading to increased lipid accumulation. Vitamin D3 deficiency has been known in obesity and related diseases. In this study we investigated the impact of vitamin D3 supplementation with and without calcium on lipid metabolism in presence of exogenous fatty acid supply on 3T3‐L1 and HepG2 cell lines. Triglyceride assay and oil o red assay reveled that the lipid accumulation on both cell line was increased with the supplementation of Vitamin D3 at a dose on 50nM, 75nM and 100nM when compared with control group. MTT study showed that cell viability was not affected by Vitamin D supplementation. Further real time PCR analysis supports that vitaminD3 alters the mRNA expressions of SREBP, GLUT4, and PPAR‐gamma along with CPT‐1alpha, UCP‐1, and HSL. These results suggest that vitamin D3 plays a crucial role in lipid metabolism.