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Activation of P2X‐purinergic receptors in the lateral parabrachial nucleus increases renal sympathetic nerve discharge and mean arterial pressure in anesthetized rats (686.13)

The lateral parabrachial nucleus (LPBN) is an important area involved in cardiorespiratory control. ATP is an important neurotransmitter and purinergic receptors are present into LPBN. The involvement of purinergic mechanisms into LPBN in the control of mean arterial pressure (MAP) and renal sympathetic nerve discharge (RSND) is unknown. We investigated the effect of alpha, beta‐me ATP (P2X agonist) alone or following pyridoxalphosphate‐6‐azophenyl‐2',4'‐disulfonic acid (PPADS, P2 antagonist) injected into LPBN on MAP and RSND in rats. Anesthetized, vagotomized and mechanically ventilated male rats (n=3) were used. Unilateral injections of alpha, beta‐me ATP (2.0 nmol/50 nl) into LPBN increased MAP (Δ 12±2 mmHg, p<0.05) and RSND (Δ 32±8%), without changing heart rate (HR). Unilateral injection of PPADS (0.125 nmol/50 nl) into LPBN did not change MAP, HR and RSND, however, pre‐treatment with PPADS into LPBN (10 min before) abolished the pressor response (Δ 2±0.8 mmHg, p<0.05) and reduced the RSND increase (Δ 12±7%, p<0.05) produced by alpha, beta‐me ATP injected into LPBN. In 2 rats responses to alpha, beta‐me ATP recovered to control within 30 min while in the remaining rat the response had recovered 31% by 70 min. These results suggest that alpha, beta‐me ATP acting on P2X purinergic receptors into LPBN produces pressor and sympathoexcitatory responses in anesthetized rats.