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Immunohistological detection of interleukin 1‐like molecules and tumour necrosis factor in human epidermis before and after UVB‐irradiation in vivo

Skin biopsies from five healthy subjects, taken before and after UVB irradiation, were examined using immunohistological techniques for the cytokines interleukin‐1 (IL‐1) and tumour necrosis factor (TNF). Using polyclonat specific antibodies against IL‐1 and TNF, the two cytokines appeared identically located on the epidermal cell membranes of the stratum granulosum and stratum spinosum in unexposed skin. After UVB‐exposure, the staining intensity for both IL‐1/epidermal cell derived thymocyte‐activating factor (ETAF) and TNF was markedly increased, and the epidermal staining included the basal cell layer.