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Rapid and Highly Sensitive Method for Protein Determination in Biological Samples with m‐Nitrophenylfluorone‐Mo(VI) Complex as a Spectral Probe

Abstract A simple, rapid, highly sensitive, and selective method for detecting protein in biological samples has been developed, which is based on the interaction between protein and m‐nitrophenylfluorone‐Mo(VI) complex as a spectral probe. The optimum condition for the reaction is investigated. Bovine serum albumin (BSA) obeys Beer's law up to 10 µg · mL−1, having a molar absorption coefficient of 8.51×106 L · mol−1 · cm−1 at 535 nm. Many amino acids and metal ions do not interfere. The results of determination for biological samples are comparable to those obtained by the Bradford method. Meanwhile, the binding number is also determined.