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Studies on the α-glucosidase inhibitory effect and antioxidant activity of total flavonoids from hypericum Ascyron L

The inhibitory effect on α-glucosidase of total flavonoids from Hypericum Ascyron L.( HAF) was investigated through the testing of inhibition rate and inhibition kinetics. The antioxidant activities of the flavonoids were evaluated in vitro by scavenging capability of DPPH free radical,the bleaching trials of betacarotene,and the protecting of DNA oxidative damage. The result showed that the HAF was a reversible mixed inhibitor,and its half inhibition constant IC 50 was 0. 237 mg / mL,the inhibition constant K i to α-glucosidase was 0. 149 mg / mL,and inhibition constant to the enzyme-substrate complex K is was 0. 123 mg / mL,respectively. The antioxidant assay indicated that the HAF had significant DPPH radical scavenging activity( IC 50 = 0. 27 μg / mL),inhibition capacities on bleaching of β-carotene and DNA oxidative damage,which should be further investigated and utilized as a potential antioxidant and therapeutic drug of type Ⅱ diabetes mellitus or dietary function factors.