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Evaluation of a rapid competitive protein binding assay for progesterone in pregnancy and some of its complications.

The competitive protein binding (CPB) method for progesterone assay was evaluated on 206 blood samples from normal pregnant women collected at various stages of gestation. Corticosterone binding globulin (CBG) was extracted from the plasma of normal females treated with ethinyl estradiol for 14 days. Since testosterone 20-alpha-hydroxyprogesterone 17-alpha-hydroxyprogesterone and corticosterone can also be bound by CBG the choice of specific solvents is indicated. Serum progesterone concentrations in the first trimester of pregnancy (19.1 ng/m) were slightly higher than the levels during the luteal phase of the menstrual cycle. By the third trimester it was 135.3 ng/m and 156.9 ng/m at term. In hydatiform mole ovarian theca lutein cysts secrete quantities of 20-alpha-hydroxyprogesterone and 17-alpha-hydroxyprogesterone. The CPB method is simple sensitive fast and economical.