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(-) mating type-specific mutants of Phycomyces defective in sex pheromone biosynthesis.

We have isolated the first mating type-specific mutants in mucoraceous fungi. Both mutants in Phycomyces blakesleeanus appear to be defective in the same gene. The gene, present in both mating types, is necessary only in cultures of the (-) mating type. The gene codes for an enzyme in sex pheromone biosynthesis. The pheromone precursor made by the mutants is detectable only in cross-feeding experiments. The biological and solubility properties of the precursor suggest the precursor is 4-dihydrotrisporin, a metabolite of beta-carotene. Separate studies with beta-carotene-deficient mutants and Compound-P, a new chemically synthesized precursor of the pheromones, imply the constitutive level of enzymes for pheromone biosynthesis in Phycomyces is extremely low. In comparison, the level of enzymes for pheromone conversion to trisporic acid is higher. The mating type-specific mutants also catalyze the conversion of (+) pheromone to trisporic acid. This finding was unexpected because literature models predicted this reaction was catalyzed by the same enzyme which catalyzed the conversion of 4-dihydrotrisporin to (-) pheromone-a reaction missing in the (-) mating type-specific mutants. Thus, we propose a revised model for trisporic acid biosynthesis.