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Determination of chlormezanone in human plasma after administration of chlormezanone formulations.

In the course of a bioavailability study some 800 plasma samples were analysed by means of HPLC after administration of 5 different chlormezanone formulations to 10 healthy male volunteers. A metabolic product of chlormezanone (I) could not be detected in any of the samples. The results were further verified through mass spectrometric measurements of chlormezanone, extracted from the plasma sample and separated through HPLC, in off-line mode. Chlormezanone is found as an intact molecule in human plasma samples.