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Detection of measles virus RNA on SYBR green real‐time reverse transcription–polymerase chain reaction

Background:  As the coverage rate of the measles vaccine increases, not all patients present the typical symptoms of measles after exposure to the measles virus (MV). The virus loads in clinical specimens from patients with vaccine‐modified non‐typical measles are expected to be low compared with those of primary MV infection. A rapid and sensitive laboratory procedure is required for diagnosis of measles.