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Standardization of Siddha Poly-Herbal Formulation Thirinethira Chooranam by Modern Pharmaceutical Analytical Techniques

Thirinethira Chooranam is a multi-herbal formulation indicated as hematinic and appetizer in Siddha literature. The aim of this study is to standardize Thirinethira Chooranam to ensure its quality, purity and safety through its physicochemical, microbiological, chromatographic, biochemical parameters and analysis of heavy metals, Pesticide residue and aflatoxins. This study determines the physico-chemical parameters that are loss on drying, total ash, acid insoluble ash, water soluble extractive, alcohol soluble extractive and pH as 2.187%, 6.9 %, 0.18%, 14.17%, 4.567% and 5.5 respectively denotes purity and quality of drug. From the study results it is ensured that the test drug Thirinethira chooranam is free from microbial contamination, heavy metal traces, Pesticide residue toxicity and aflatoxin toxicity. Qualitative preliminary Phyto-chemical analysis reveals that presence of major phytochemicals alkaloids, flavonoids, steroids, triterpenoids, coumarins, phenol, tannin, sugar and betacyanin. High-Performance thin layer chromatography finger printing analysis of the test drug reveals the presence of seven eminent modes relevant to presence of seven different phytocomponents present with in it. Rf value of the modes ranges from 0.01 to 085. Bio-chemical analysis reports the presence of carbonate, sulphides, phosphate, ferrous and magnesium. The obtained results of all the analyses of this study provide data on pharmacognostic (physical, chemical and biochemical) properties of siddha poly-herbal formulation Thirinethira Chooranam. In future these data can be utilized as references for the standardization of the drug Thirinethira Chooranam.

Key words: Thirinethira Chooranam, Siddha, poly-herbal formulation, standardization.