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Processing oxidatively damaged bases at DNA strand breaks by APE1

Abstract Reactive oxygen species attack the structure of DNA, thus altering its base-pairing properties. Consequently, oxidative stress-associated DNA lesions are a major source of the mutation load that gives rise to cancer and other diseases. Base excision repair (BER) is the pathway primarily tasked with repairing DNA base damage, with apurinic/apyrimidinic endonuclease (APE1) having both AP-endonuclease and 3′ to 5′ exonuclease (exo) DNA cleavage functions. The lesion 8-oxo-7,8-dihydroguanine (8-oxoG) can enter the genome as either a product of direct damage to the DNA, or through polymerase insertion at the 3′-end of a DNA strand during replication or repair. Importantly, 3′-8-oxoG impairs the ligation step of BER and therefore must be removed by the exo activity of a surrogate enzyme to prevent double stranded breaks and cell death. In the present study, we use X-ray crystallography to characterize the exo activity of APE1 on 3′-8-oxoG substrates. These structures support a unified APE1 exo mechanism that differs from its more canonical AP-endonuclease activity. In addition, through complementation of the structural data with enzyme kinetics and binding studies employing both wild-type and rationally designed APE1 mutants, we were able to identify and characterize unique protein: DNA contacts that specifically mediate 8-oxoG removal by APE1.


INTRODUCTION
During times of oxidative stress, excess cellular reactive oxygen species (ROS) react with and modify the structure of nucleic acids both within the DNA and the free nucleotide pool (1)(2)(3). These modifications induce mutations in the genome and are a major source of the genomic instability that promotes multiple human diseases, such as can-cer. The base excision repair (BER) pathway has evolved as the cell's primary defense against base lesions and single stranded breaks (SSB) that arise during oxidative stress ( Figure 1A) (4)(5)(6)(7)(8). The BER pathway consists of five major steps: (i) recognition and excision of a damaged base, (ii) strand cleavage at the abasic site, (iii) replacement of the excised DNA nucleotide, (iv) processing of DNA ends and (v) sealing of the DNA nick. Within this pathway, APE1 processes DNA damage with two distinct nuclease activities: AP-endonuclease (AP-endo) and 3 to 5 exonuclease (exo). APE1 cleaves the DNA backbone 5 of the abasic site with its AP-endo activity. In contrast, the exo activity of APE1 proofreads and processes aberrant 3 DNA termini that can prevent faithful repair, see red arrows in Figure 1A.
Oxidatively damaged DNA termini can be referred to as DNA 'dirty ends' and require subsequent processing to repair the lesion and maintain genome stability (9). One major oxidized nucleobase resulting from ROS is 8oxo-7,8-dihydro-2 -deoxyguanosine, which is found in both DNA (8-oxoG) and in the nucleotide pool (8-oxodGTP) (1)(2)(3). Even with cellular sanitizing activities, nucleotide pools contain enough 8-oxodGTP to promote mutagenesis (10,11). Mutagenesis arises from the dual coding potential of the oxidative lesion, where 8-oxoG (anti) base pairs with cytosine (C) using its Watson-Crick face and 8-oxoG (syn) uses its Hoogsteen face to base pair with adenine (A), Figure 1B. Structural studies examining the insertion of 8-oxodGTP revealed that the modified nucleotide can escape general DNA polymerase discrimination checkpoints by modulating the highly charged DNA polymerase active site (3,12). Importantly, a DNA ligase would be responsible for sealing a nick with a dirty end. Unfortunately, 8-oxoG positioned at the 3 -end of a nick hastens abortive ligation and stabilizes the cytotoxic nick, thus resulting in persistent DNA containing SSBs with 3 -terminal 8-oxoG ends necessitating extrinsic end processing (3,13,14).
APE1 has been shown to be the major 3 -exonuclease activity responsible for the repair of 3 -8-oxoG lesions in human cells (13). APE1 end processing of 8-oxoG, during which the oxidative DNA lesion is removed, results in a 'clean' 1-nt gapped substrate suitable for DNA synthe- sis by Pol ␤ during BER ( Figure 1A). Importantly, yeast mutants lacking APN1 (the yeast homolog of APE1) are hypersensitive to DNA-damaging agents (15). Moreover, these mutants display a mutator phenotype characterized by a 60-fold increase rate of A:T to C:G transversions (16). Consistent with APN1 playing a key role in removing 3 -8-oxoG, the rate of these transversion mutations is strongly supressed in ogg1 mutants (17). Currently, the molecular mechanism used by APE1 for the 3 end processing of the 8-oxoG lesion is unknown. To probe this mechanism, we combined X-ray crystallography and enzyme kinetics to observe and characterize APE1 cleaning dirty DNA ends that arise from oxidative stress.

Expression and purification of APE1
Both human wild-type (full length) and APE1 (lacking 43 N-terminal amino acids) were expressed in the absence of any tags from pet28a codon optimized clones purchased from GenScript. To generate the full-length and APE1 variants, site-directed mutagenesis was performed with a QuickChange II site-directed mutagenesis (Agilent). Once confirmation of correct sequencing was obtained, the plasmids were transformed into One Shot BL21(DE3)plysS Escherichia coli cells (Invitrogen). Cells were grown in 2× YT at 37 • C until OD 600 was 0.6. The cells were then induced using isopropyl ␤-D-thiogalactopyranoside (IPTG) to a concentration to 0.4 mM. The temperature was then reduced to 20 • C and cells were allowed to continue growing while shaking overnight before being harvested. After harvesting, cells were lysed at 4 • C by sonication in 50 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM EDTA, 1 mM DTT and a protease inhibitor cocktail (1 g/ml leupeptin, 1 mM benzamidine, 1 mM AEBSF, 1 g/ml pepstatin A). Lysate was clarified from cell debris by centrifugation for 1 h at 24 424 × g. The pellet was discarded, and the resulting supernatant was passed over a HiTrap Heparin HP column (GE Health Sciences) equilibrated with lysate buffer (50 mM HEPES, pH 7.4, 50 mM NaCl). APE1 was eluted from the column using a linear gradient of NaCl up to 600 mM. APE1 was then buffer exchanged into 50 mM NaCl and loaded onto a Resource S column (GE Health Sciences) equilibrated in lysate buffer (50 mM HEPES, pH 7.4, 50 mM NaCl). APE1 was eluted from the column using a linear gradient of NaCl up to 400 mM. APE1 was subsequently loaded onto a HiPrep 16/60 Sephacryl S-200 HR (GE Health Sciences) equilibrated in 50 mM HEPES, pH 7.4, and 150 mM NaCl. SDS-PAGE was used to determine purity of the resulting fractions. Pure fractions were pooled together, and the final concentration was determined by a NanoDrop One UV-Vis Spectrophotometer (Thermo-Scientific) at an absorbance of 280 nm. Protein was aliquoted and stored at -80 • C in a buffer of 50 mM HEPES, pH 7.4 and 150 mM NaCl.

APE1 crystallization and structural determination
To generate the substate DNA for the 3 -8-oxoG opposite A and C structures, three oligonucleotides (see DNA Sequences above) were annealed in a 1:1:1 ratio to a final concentration of 2 mM using a PCR thermocycler by heating for 10 min at 90 • C and cooling to 4 • C (1 • C/min). In both cases, the annealed DNA was mixed with C138A-APE1 to achieve a final concentration of 0.56 mM DNA and 10-12 mg ml −1 C138A-APE1. The single amino acid C138A mutation and truncation of the N-terminal 43 amino acids aid in crystallization (18,19). Vapor diffusion was utilized to crystalize the APE1: DNA complexes. The reservoir solution for crystal formation was 7-14% PEG 20 K, 100 mM sodium citrate, pH 5.0, 15% glycerol and 5 mM calcium chloride. Crystals formed within a week at 20 • C and were transferred to a cryosolution containing the mother liquor and 25% ethylene glycol. Data were collected at 100 K on a Rigaku MicroMax-007 HF rotating anode diffractometer equipped with a Dectris Pilatus3R 200K-A detector system at a wavelength of 1.54Å. This allowed for anomalous data detection after phasing by molecular replacement with high redundancy. Data were processed and scaled with the HKL3000R software package (20). Initial models were determined by molecular replacement with a modified version of a previously determined APE1-DNA complex (5WN4) as a reference. Refinement was carried out with PHENIX and model building with Coot (21,22). The figures were prepared with PyMOL (Schrödinger LLC), and for simplicity only one conformation is shown for residues with alternate conformations unless otherwise noted.

APE1 exonuclease activity assays
APE1 exonuclease DNA cleavage activity assays were performed using a benchtop heat block set to 37 • C. DNA substrates used to measure APE1 incision activity contained a 5 ,6-carboxyfluorescein (6-FAM) label and were designed with a centrally located nick flanked by a 3 -8oxoG and a 5 -phosphate. Full-length, untagged, wild-type APE1 was used in all activity assays, except where specific mutations are indicated. The reaction buffer was 50 mM HEPES, pH 7.4, 100 mM KCl, 3 mM MgCl 2 and 0.1 mg ml -1 BSA. Final concentrations after mixing were 100 nM DNA substrate and 0-250 nM APE1, as indicated on the plots. After 30 min the reactions were quenched by mixing with an equal volume of DNA gel loading buffer (100 mM EDTA, 80% deionized formamide, 0.25 mg ml -1 bromophenol blue and 0.25 mg ml -1 xylene cyanol). After incubation at 95 • C for 5 min, the reaction products were separated by 22% denaturing polyacrylamide gel. The indicated % product formation for each concentration of APE1 are the mean of three independent experiments. A GE Typhoon FLA 9500 imager in fluorescence mode was used for gel scanning and imaging, and the data were analysed with ImageJ software (23).

Anisotropy binding studies
Fluorescence anisotropy measurements were used to quantify binding of full length, untagged, wild-type APE1 to the FAM-labelled DNA substrates described above. Fluorescence anisotropy measurements were carried out on a Horiba FluoroLog Fluorimeter at 37 • C in a buffer consisting of 50 mM HEPES pH 7.4, 100 mM KCl, 5% (v/v) glycerol and 20 mM EDTA. The excitation and emission wavelengths were 485 and 520 nm, respectively, each with a 5 nm bandpass. For all titrations, the concentration of the FAM-labelled DNA was 10 nM to stay below the K D and maintain sufficient signal to noise. Fluorescence anisotropy changes were fit by least squares regression to a one-site specific binding model using with Prism (GraphPad). A minimum of ten independent measurements were averaged for each APE1 concentration of the titrations to determine K D values.

APE1 engaged with exonuclease substrates containing 3 -8-oxoG lesions
The 8-oxoG base has been shown to adopt a variety of conformations both within protein active sites and in duplex DNA. This arises from the dual coding potential of the 8-oxoG base, which can form stable base pairs with both cytosine utilizing its Watson-Crick face and adenine using its Hoogsteen face ( Figure 1B). Moreover, DNA Pol ␤ inserts 8-oxodGTP opposite A, forming a mutagenic base paring, and C, forming a non-mutagenic base pairing, with only minor discrimination (3). To see how the APE1 active site accommodates 3 -8-oxoG, we crystallized APE1 bound with nicked, double stranded DNA substrates containing 8-oxoG at the 3 -end and a phosphate at the 5end of the SSB. These substrates represent potentially unligatable (14), BER intermediates in which the repair polymerase, Pol ␤, has misinserted an 8-oxodGTP across from either a templating A or C. To obtain crystals of the substrate complexes, we employed a double-mutant catalytically dead variant of APE1 (E96Q/D210N) (3,24). The resulting structures, Table 1, revealed the APE1 active site positioned to remove the 8-oxoG lesion from the 3 end of the DNA nick using its exo activity. Both the overall struc-  (Figures 2A and C). Of note, density for the 8-oxoG base is relatively weak, for the structure with 8-oxoG opposite C. We attempted, but were unable to obtain, crystals of APE1 with an analogous uncleavable (i.e. phosphothioate containing) substrate. We have, however, determined structures of APE1 bound to other exo substrates in the past with uncleavable DNA and the density for the base remained weak (e.g. PDB ID 5WN4). Therefore, it is likely that the relatively weak density for the 8-oxoG base is the result of high mobility, as opposed to low occupancy. Supporting this, the B-factors for the 3 -8-oxoG, as well as the 5 -base and opposing base, are higher in the case of 8-oxoG opposite C (Supplemental Figure S1). This high mobility in the DNA region around and including the 3 -8-oxoG base likely results from the lack of WC base paring between the 3 -8-oxoG base and the opposing base and the open active site pocket in which the 8-oxoG nucleobase resides. The 3 -8-oxoG structures also reveal several key protein:DNA contacts. These regions include DNA intercalating residues, a hydrophobic pocket composed of F266 and W280 adjacent the 3 -8-oxoG, stabilizing contacts with the 5 -phosphate, and the catalytic residues ( Figures 2B and  D). R177 and M270 intercalate the DNA by wedging between the 8-oxoG base and the opposing A or C, serving as a physical block to any potential hydrogen bonding between 8-oxoG and the opposing base. Additional contacts are formed between the 5 phosphate and the side chains of N222, N226, and W280 and two stable water molecules. Moreover, importantly, the DNA backbone is in position for catalysis adjacent residues composing the catalytic triad (E96, N68, and D210), Y171 and D70.  Opposite either A or C, the 8-oxoG base adopts an anticonformation, in which the Watson-Crick face of the base is oriented toward the major groove and the 8-oxoG base is accommodated via an open protein: DNA intra-helical binding pocket, Figure 3. M270 and R177 border the 'DNA side' of the binding pocket, which is composed of hydrophobic residues F266 and W280. This contrasts with the endonuclease activity of APE1 with abasic DNA, of which the abasic site is base-flipped within the APE1 active site to adopt a catalytically competent conformation for cleavage during the AP-endo reaction (3,25). The open active site accommodating 8-oxoG results from several structural variations between the 3 -8-oxoG exo and AP-endo structures, including a 10 • sharper bend in the DNA and displacement of the backbone phosphate located 5 of the nick. Of note, these structural differences are also observed in APE1 exo structures with a 3 -mismatched DNA substrate (4).
A closer look at the structure with 8-oxoG opposite A reveals the phosphate backbone oxygen (O5 ) is coordinated to N174 (ND2) and N212 (OD1), Figure 4A. The 3 -OH of the sugar is also coordinated to N212 (OD1), while the two non-bridging oxygens are coordinated by N212 (ND2), N210 (ND2), Y171 and a water molecule. A second, ordered water molecule is in position to act as the nucleophile. This water is coordinated by the phosphate backbone oxygen (O5 ) as well as one of the non-bridging oxygens, positioning the water atom 2.9Å away from the phosphorus atom ( Figure 4A). Moreover, the nitrogen atom of N210 (ND2) and the other active site water molecule are within H-bonding distance of the nucleophilic water at 2.7 and 3.1 A, respectively. Also observed is a hydrogen bonding network between Q96, D70, N68, N210 and N212 that likely alters the pKa of N210 (D210 in WT APE1), thus facilitating the deprotonation of the water and subsequent nucleophilic attack at the phosphate backbone during cleavage. These contacts are consistent with a water mediated nucleophilic attack mechanism (25)(26)(27). However, the structure lacks an anticipated bound active site metal ion, which is required for the APE1 exo activity (26,28). This is most likely due to the APE1 D210N/E96Q active site mutations, which render a catalytically dead triad where Q96, N68 and N210 undergo moderate rotameric shifts to hydrogen-bond with one another (3). This prevents E96Q from coordinating the metal, because NE2 is pointed toward the metalbinding pocket, and OE1 coordinates ND2 of N68. A similar phenomenon occurs with the N210 substitution, which coordinates OD1 of N68, thus resulting in ND2 coordinating the nucleophilic water molecule. Similar contacts occur between the APE1 protein side chains and the phosphate backbone of 3 -8-oxoG opposite C, Figure 4C. Overall, the organization of the APE1 active site around the DNA substrates points to a unified mechanism for APE1 exo activity on 3 mismatches and 3 end damages including both the small oxidative lesion PG and the bulkier lesion 8-oxoG (4).
In both structures, the 8-oxoG base is in position to hydrogen bond to the 5 -P and makes several contacts with the protein active site (Figures 4B and D). However, the precise contacts with the 8-oxoG base vary more substantially between the two structures than those between the backbone ( Figure 4) likely due to its closer proximity to the variable opposing base, of which the double ring structure of A is larger than the single ring structure of C. Another similarity between the two structures is the intercalation of the DNA helix by the APE1 active site residue R177, which interacts with the Watson-Crick face oxygen (O6) of 8-oxoG using two of its side chain nitrogen atoms (NH2 and NE), Figure 4B and D. Of note, the O6 oxygen atom of 8-oxoG in the O8:A structure can additionally H-bond with a nearby stable water ion, Figure 4B. Positioned in between the two strands of the DNA helix, R177 is also in position to hydrogen bond with the opposing A or C base. These interactions between R177 and the opposing bases result in differences in its interactions with the 8-oxoG base. Specifically, the hydrogen bonding between R177 (NH2) and the 8-oxoG O6 oxygen atom is weaker, 3.0 versus 2.6Å, in the case of the O8:C structure due to the contact between R177 and C requiring R177A to shift further towards the opposing strand than when it is opposite A (Figures 4B and D). Stabilizing the other side of the 8-oxoG base, N174 is in position to form H-bonds with the lesion's unique adducted oxygen atom (O8). N174 sits closer to the O8 of O8:A with 2.5Å between it and the nitrogen (ND2) of the N174A side chain, however in the case of O8:C both the N174 OD1 and ND2 are within hydrogen bonding distance at 2.9 and 3.0Å, respectively ( Figures 4B and D). The 8-oxoG bases of both structures also form H-bonds with the 5 end of the DNA nick, with those in O8:A structure possibly being more extensive. These varying stabilizing interactions between the two structures is also reflected in the structural B-factors, which vary most substantially at the 3 -8-oxoG and opposing base positions (Supplemental Figure S1). These differences are likely due to the shift in position of R177 between the two structures, which also pulls the 8-oxoG base itself along with it toward the opposing strand allowing it to maintain contact with both the opposing and 8-oxoG bases.

Biochemical characterization of APE1 and variants with 3 -8-oxoG substrates
To gain further insight into the substrate specificity of APE1 on 3 -8-oxoG substates, we examined the ability of full-length, wild-type APE1 to both bind and perform its exonuclease activity (3 -8-oxoG removal) on substates containing either A or C as the base opposing the 3 -8-oxoG ( Figure  5). Importantly, these substates represent mutagenic (O8:A) and non-mutagenic (O8:C) base pairings. To determine the equilibrium binding affinities between APE1 and the 3 -8-oxoG substates, we measured fluorescence anisotropy of the DNA at increasing concentrations of APE1 from 0 to ∼4000 nM and fit the resulting binding curve to a least squares fit non-linear regression ( Figure 5A). The data indicate that APE1 binds the two substates, O8:A and O8:C, with similar affinities (K D ) of 165.7 ± 31.38 nM and 205.6 ± 28.60 nM, respectively. To determine the relative exo activity of APE1 on these two substates, we next performed APE1 activity assays. In these experiments, we observed APE1 product formation after 30 min over a range of APE1 concentrations ranging from 0.5 to 250 nM for each substrate. At each concentration of APE1, more product was observed for the O8:A substrate, opposed to O8:C, with about 2-fold more product formation for this mutagenic base pairing at the highest concentrations of APE1 ( Figure 5B). Overall, the exo activity on both 3 -8-oxoG substrates is less efficient than that observed on a C:T mismatch, Supplemental Figure S2.
Previous work characterizing the exo activity of APE1 on a 3 -mismatched substrate demonstrated that mutating the residues that compose the enzymes hydrophobic pocket, W280 and F266, to alanine increases the steady-state rate of APE1 exo activity (25,29) by 5-fold and 50-fold, respectively. A structure of the F266A APE1 variant engaged with a 3 -mismatched substrate indicated that the increase in activity was due to an expansion of the active site pocket, allowing the mismatched C to occupy an alternate, more catalytically active, conformation. The similarities between previous 3 -mismatched structures and the 3 -8-oxoG structures described above indicate that APE1 utilizes an analogous mechanism for its exo activity on these substrates as it does for 3 -mismatched substrates. To probe the role of these key active site pocket residues, we next utilized the APE1 cleavage activity assay on the same 3 -8-oxoG containing substates used above with W280A and F266A APE1 variant enzymes (Figures 6A and B). Our structures indicate that the side chains of both W280 and F266 constrict the size of the APE1 active site, and previous kinetic analyses indicate W280A and F266A APE1 mutants increase the exo catalytic rate (4,29). In agreement, F266A showed a dramatic increase in the rate of product formation compared to WT APE1 for both O8:A ( Figure 6A) and O8:C ( Figure 6B). This indicates a universal role for F266 and the APE1 intrahelical binding pocket in APE1 exo activity. W280A, which modestly enhances exo activity in the case of a 3 -mismatch in comparison to WT APE1, on the other hand, modestly reduces the APE1 catalytic activity on exo substrates with a 3 -8-oxoG (Figures 6A and B). An overlay of the crystal structure of APE1 with 3 -mismatched C in the intrahelical binding pocket (PDB:5WN4) with those containing 3 -8-oxoG indicate a rotation in 8-oxoG of about 60 • relative to the position of the 3 -mismatched C away from W280, perhaps explaining the different catalytic roles of W280 ( Figure 6E). This rotation of the 8-oxoG base allows for the interaction between the adducted O8 oxygen and the side chain of N174.
The 8-oxoG base makes unique interactions with the APE1 N174 and R177 side chains. To determine whether these interactions play a role in the APE1 exo activity on 3 -8-oxoG substates, we performed the same APE1 product formation assays with N174A and R177A APE1 variants ( Figures 6C and D). Irrespective of the opposing base, N174 is in position to H-bond with the adducted oxygen of the 8-oxoG base (Figures 4B and D). When this interaction is lost, via mutation of N174 to an alanine, we observed APE1 to have reduced activity on both 3 -8-oxoG substates. R177 interacts with both opposing bases and the 8-oxoG base, and because the structure of the opposing bases (C versus A) varies, these interactions result in differences in the interactions between R177 and the 8-oxoG base. Moreover, R177 is thought to be an important residue for the product release step of APE1 cleavage, and when mutated to an alanine increases APE1 endo activity as well as its exo activity on a 3 -mismatch (3,4,30,31). Strikingly, we observed R177A to have an opposite effect on 3 -8-oxoG substates, with dramatically decreased activity on both 3 -8-oxoG substates. This indicates that the R177 sidechain is critical for APE1 removal of the 8-oxoG lesion from the 3 -end of a DNA nick. While the structures demonstrate M270 to not make any direct contact with the DNA, it sits in position to, along with R177A, prevent base pairing interactions between 3 -8-oxoG and the opposing base, Figure 3B. Therefore, considering the surprising decrease in activity of the R177A APE1 variant on 3 -8oxoG substrates, we choose to also characterize the exo activity of the other DNA intercalating residue, M270A, on our 3 -8-oxoG substrates. In these assays, product formation by M270A was more efficient than that observed by R177, but substantially diminished compared to WT APE1 for both 8-oxoG containing exo substrates (Figures 6C and D). Thus, these results provide further evidence these two DNA intercalating residues play a key role in the APE1 exo activity on 3 -8-oxoG substates.

DISCUSSION
Both oxidative DNA damage itself and its repair mediate the progression of many human diseases, including cancers, associated with genomic instability (18). As a result, efficient BER plays a vital role in maintaining genomic integrity. On the other hand, BER failure is both cytotoxic and potentially mutagenic, thus contributing to the pathology of disease (18,(32)(33)(34). Akin to genomic DNA, dNTPs are also vulnerable to oxidation by ROS, leading to the presence of their oxidized forms (e.g. 8-oxodGTP) in the nucleotide pool. During BER, oxidized nucleotide insertion by Pol ␤ leads to ligation failure and the formation of a blocked 5 -adenylated, cytotoxic repair intermediate (14). Importantly, because of the genomic instability resulting from their incorporation into the DNA during replication and repair, elevated levels of 8-oxodGTP and other oxidized nucleotides are associated with disease and cancer (35,36). Moreover, because Pol ␤ lacks a proofreading domain, misinsertions must be removed by a surrogate enzyme(s) to avoid genomic instability. Prior to this study,  APE1 was shown to play this role, and can remove 3 -8-oxoG from nicked DNA substrates in vitro (13,14). Moreover, cellular studies using whole cell extracts and immunoprecipitation experiments also support a cellular role of APE1 in 3 -8-oxoG removal (13,37,38). However, the mechanism of APE1-mediated removal of 8-oxoG from the 3end of a DNA SSB previously remained elusive.

The conformation of 8-oxoG in the APE1 active site is independent of the opposing base
One thing revealed by the substrate structures of APE1 engaged with a 3 -8oxoG, is that the 8-oxoG base adopts an anti-conformation independent of the nature (i.e. A or C) of the opposing base (Figures 2 and 3). This is distinct from the variable conformations of 8-oxoG observed when the base lesion is in duplex DNA, during which 8-oxoG(anti) base pairs with C and 8-oxoG(syn) adopts the Hoogsteen conformation to base pair with A (39). The structural characterization of representative members of four families of DNA polymerases indicates that when 8-oxoG is in the flexible template binding pocket it can typically adopt either an anti or syn conformation depending on the nature of the incoming nucleotide as either C or A, respectively (40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50). In contrast, as an incoming nucleotide, 8-oxodGTP usually favours the mutagenic syn conformation due to a lack of flexibility in the incoming nucleotide binding pocket and steric repulsion between O8 and its deoxyribose-phosphate (12,40,51,52). Moreover, the insertion of 8-oxodGTP by Pol ␤ has been characterized utilizing time-lapse X-ray crystallography (3). This is particularly relevant, as it is this misinsertion of 8-oxodGTP by Pol ␤ that proceeds its removal by the 3 to 5 exo activity of APE1. Interestingly, 8-oxoG insertion by Pol ␤ across from C and A revealed 8-oxoG to be in either the anti or syn conformation during the reaction, respectively. However, with both templating bases, as Pol ␤ reopened after catalysis the hydrogen bonding interactions between the bases were lost, and both adopted anti-conformations resembling those observed in the APE1 active site. This lack of stable base pairing between 3 -8-oxoG(anti) and either opposing base prior to APE1 engagement with the nicked DNA likely favours formation of the catalytically competent APE1:3 -8-oxoG(anti) conformations observed in our structures. In agreement with this, 3 -mismatches are removed by the APE1 exo activity more efficiently than matches (26,53) and the efficiency of their removal is directly correlated to the thermostability of the mismatched base pair at the 3 -end of the nick (54). Moreover, this also provides rationale for the apparent lack of strong discrimination depending on the opposing base, <2fold ( Figure 6).

APE1 utilizes an intra-helical hydrophobic binding pocket to accommodate the 3 -8-oxoG
While the above discussion addresses why there is not a large difference in APE1 activity depending on the nature of the opposing base, other factors must contribute to the modest difference in the APE1 exo activity observed between the two substates ( Figure 5). To probe both these differences and furthermore to compare APE1 3 -8-oxoG removal with the other nuclease activities of APE1, we also characterized the 3 -8-oxoG exonuclease activities of a set of APE1 variants. Of the five variants, four (N174A, W280A, M270A and R177A) resulted in reduced APE1 product formation while F266A, located in the intra-helical hydrophobic binding pocket, increased the rate of 3 -8-oxoG cleavage. The dramatic increase in APE1 exo activity on 3 -8-oxoG substrates in response to the F266A mutation (Figures 6A and B) was also observed previously in the case of 3 -mismatched substrates and was contributed to the mutation generating sufficient space in the APE1 active site for the 3 -base to adopt an alternative, more favourable, conformation (26,29). Interestingly, F266A does not have the same effect on the APE1 AP-endo reaction and represents a separation of function mutant for APE1 exo and endo activities. The other hydrophobic pocket residue we tested, W280A, showed only a slight decrease in APE1 activity. Interestingly, the opposite effect was seen in the case of 3 -mismatched exo substates (26); however, in both cases the magnitude of change in response to the mutation was small, and it appears that the role played by W280 in APE1 exo reactions is minimal and is 3 -base-dependent in nature. Combined, the present study and previous work examining APE1 exo activity on 3 -mismatches indicate that the restrictive APE1 active site, and steric burden of large exo substrates (relative to an abasic site), are unifying features of the APE1 exo mechanism, and are likely responsible for the overall reduced incision rates for APE1 exo reactions compared to AP-endo reactions. Of note, the hydrophobic binding pocket comprised of F266 and W280 ( Figure 3A), is not well conserved among APE1 homologs, and while it does not appear to play a role in substate specificity between the various APE1 exo substrates, it does contribute to differences in substrate specificity between the 3 -exo and APendo activities of APE1 and the other Exo III family members (26,29).

Unique contacts between APE1 and the 8-oxoG base aid in its removal
The N174A and R177A APE1 variants were selected to probe the functional relevance of the interactions between their native residue side chains and the 8-oxoG base. R177 is in position to H-bond with the opposing bases and the 3 -8-oxoG ( Figures 2B and D), and in combination with M270 blocks interactions from forming between the two bases ( Figure 3B). On the other hand, N174 interacts with the unique O8 of the 8-oxoG lesion. All three mutants decreased the APE1 cleavage activity on 3 -8-oxoG substates ( Figures 6C and D), with the R177A mutation having a much more significant effect. This result was striking, as R177A has been characterized to have an opposite effect (albeit more modest), enhancing APE1 nuclease activity, with both AP-endo and 3 -mismatched exo substrates. This enhanced AP-endo activity of R177A APE1 has led to a model in which APE1 activity has been optimized for pathway efficiency by remaining bound to its incised product to facilitate 'hand-off' to the next enzyme in the pathway, Pol ␤ (26,55). Therefore, the role played by R177 appears to be unique in the case of 3 -8-oxoG, even among other exo substrates such as a 3 -mismatch. Moreover, previous studies examining the thermostability of 8-oxoG containing DNA indicate that the base pairs formed by 8-oxoG between A and C are both thermodynamically more stable than a C:T mismatch (56), and that this difference in stability is likely greater when the damage is located at the 3 -end (57). By intercalating the DNA helix and providing a steric barrier between the potential base pair, M270 and R177 allow the 3 -8oxoG to slide into the APE1 active site where it occupies the hydrophobic binding pocket. Thus, the importance of these residues may correlate with the flexibility of the base pair at the 3 -end.
Recent cell-based assays also explored the roll of the APE1 R177 sidechain using a complementation assay with APE1-deficient TM-Cre APE1 fl/fl MEFs and various APE1 mutants (58). Interestingly, R177A APE1 was unable to res-cue cell death. Moreover, the R177A mutant played a lesser role in protecting against MMS-induced cell death (which primarily generates abasic sites and alkylated damage) in comparison to its complementation efficiency under normal growth conditions. This may indicate that the role played by APE1 in removing the oxidative lesion 8-oxoG is particularly important for cell viability, however more work is needed to confirm this. Moreover, R177A was the only APE1 variant, including WT APE1, to have relatively the same activity on both 3 -8oxoG substrates, indicating that R177 and its interactions with the opposing base (A or C) may also be responsible for the differences in activity seen between the two substates. Therefore, R177A potentially serves as a separation of function mutation between the APE1 3 -8oxoG exo and AP-endo activities but also more specifically between its exo activities on 3 -8oxoG and its other exo activities, including 3 -mismatches.

APE1 uses a unified mechanism for its exonuclease activities
In addition to its ability to remove 3 -8-oxoG ends, APE1 is considered the major enzyme responsible for the removal of oxidatively damaged 3 -phosphoglycolate ends (59)(60)(61)(62)(63), removes 3 -end DNA mismatches from SSBs (53,64,65), as well as senses SSBs to initiates 3 to 5 end resection (66). Here, by providing novel structural and kinetic data characterizing the APE1 3 to 5 exo activity on the 3 -8-oxoG lesions, we propose that APE1 uses a unified mechanism for its exo activities on nicked substrates with 3 oxidatively damaged and mismatched DNA ends. The defining characteristics of this mechanism include DNA bending and fraying of the 3 end at the site of the nick, intercalation of the DNA by protein sidechains R177 and M270 and positioning of the 3 -base within the intra-helical hydrophobic pocket. APE1 DNA bending is part of the larger DNA sculpting mechanism used by APE1 to engage its substates (67). Supporting a unified mechanism for APE1 exo activity, APE1 similarly bends the DNA for both mismatched and 8-oxoG exo substates, but differently when bound to an abasic site ( Figure 7A). The DNA intercalating residues R177A and M270 have been implicated in multiple roles during the APE1 nuclease activity, including facilitating protein: DNA interactions (25,26,30), slowing product release (mentioned above) (3,4,25,30,31), and more recently providing steric hindrance in the active site to distinguish exo substates (68). Here we demonstrate that R177 plays seemingly different roles in the exonucleolytic cleavage of esterase activity and the 5 to 3 exo activity of 3 -8-oxoG and DNA mismatches presented here.
Highlighting the importance of APE1 during DNA repair, polymorphisms and defects in APE1 have been identified in several human populations and are associated with the development of cancer (69)(70)(71)(72)(73)(74). To this point, APE1 represents a promising cancer therapeutic target (75,76), making it essential to have a full understanding of the mechanisms of this multifunctional DNA repair enzyme. Moreover, the identification and characterization of separation of function mutants, such as F266A and R177A, is a key step in the rational design of molecular therapeutics that can specifically target select APE1 activities, including the 3 -8oxoG exo activity.

DATA AVAILABILITY
Atomic coordinates and structure factors for the reported crystal structures have been deposited with the Protein Data bank under accession numbers 7SUV (O8:A) and 7SVB(O8:C).

SUPPLEMENTARY DATA
Supplementary Data are available at NAR Online.