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Are close contacts between astrocytes and endothelial cells a prerequisite condition of a blood‐brain barrier? The rat subfornical organ as an example *

The microvessels of the rat subfornical organ (SFO) are heterogeneous: those of the caudal part lack a blood‐brain barrier (BBB) unlike those of the rostral part. The astroglial environment of these microvessels has been studied by combining an immunocytochemical technique employing an anti‐GFAP (glial fibrillary acidic protein) antiserum with the morphological detection of a barrier to the protein‐silver complex.