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Establishment and Application of an Indirect ELISA for Detecting Antibodies Against Newcastle Disease Virus

To develop a method to detect the antibody against newcastle disease virus,an indirect ELISA was successfully developed to detect anti-NDV antibody using recombinant HN protein as coating antigen.The method detected the positive sera of other four avian diseases(H9 AIV,IBV,IBDV,EDSV)were negative.Coefficients of variability percent(C V%) of intro-batch and inter-batch duplicative tests were less than 5 % and 10 %,respectively.Lowest antibody titer of positive control serum was 1∶12 800.Compared with the HI,the concordance was 96.1%.Therefore,the HN indirect ELISA has good specificity,sensitivity and repeatability,could be used as a simple and rapid serology detection method for monitoring of anti-NDV antibody and epidemiologic survey of NDV.