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Chemical Composition, Antibacterial, Anti-oxidant and Cytotoxic properties of Green Synthesized Silver Nanoparticles from Annona muricata L. (Annonaceae)

This study aims to evaluate the chemical constituents and biological potential of aqueous leaf extract and green synthesized silver nanoparticles (AgNPs) from Annona muricata. The GC-MS analysis of aqueous extract showed major phyto compounds Dioctyl Phthalate (21.23%) and Tetracosane (9.63%). We found that the AgNPs have better antibacterial activity against both gram-positive and gram-negative bacteria (more than 24 mm in diameter zone of inhibition). The DPPH free radical scavenging activity is the evident of anti-oxidant activity (70% inhibition in 100 μg/ml). In cytotoxic activity, treatment with aqueous and AgNPs caused membrane damage and cell shrinkage in a dose dependent manner with lower concentration of IC50 values 98.58 and 36.53 μg/ml against HeLa cell line. The morphological changes, membrane damage, cell shrinkage and cell death were evidenced by MTT assay, AO/EB staining and DNA fragmentation. Moreover, the AgNPs showed excellent results than aqueous extract in all the biological activities. Based on the results obtained from the present study, the biologically synthesized AgNPs can be utilizing for the medical applications in future.