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Dynamics of proteins confined in non-ionic bicontinuous microemulsions: a FCS study.

In our present work we present an approach which allows one to confine proteins in structurally nearly identical bicontinuous microemulsions with systematically decreasing water domain size. It is shown that sub-diffusive behaviour occurs already at water domain sizes below 13 nm. However, above 13 nm normal diffusion is seen. Moreover, we compare protein diffusion in microemulsions to the transport of a much smaller fluorescent dye.