{'fraction_non_alphanumeric': 0.026905829596412557, 'fraction_numerical': 0.008071748878923767, 'mean_word_length': 5.032432432432432, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Phenotypic analysis of an MgtE magnesium transporter mutation in Bacillus subtilis

Abstract. Magnesium (Mg2+) is the most abundant divalent cation in living cells and plays structural and biochemical roles in many cell processes. Although the importance of Mg2+ as a cellular nutrient has been well established, the process by which organisms obtain Mg2+ from their environment is still unclear. The Gram-positive bacterium Bacillus subtilis is used as a model system to understand Mg2+ uptake and utilization in bacteria. The B. subtilis genome codes for a homolog of the MgtE family of Mg2+ transporters. The protein is 34% identical to the structurally well-characterized MgtE homolog in Thermus thermophilus. This study was designed to characterize phenotypes of a B. subtilis mutant deleted for the mgtE gene. Compared to wild type, the ΔmgtE strain exhibits a Mg2+-dependent growth defect, consistent with a role of MgtE in Mg2+ transport. Additionally, mutant cells are more resistant to manganese, show a decrease in sporulation efficiency, and are more sensitive to hydrogen peroxide than wild type cells.