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Release of prostaglandin Es by hypothalamic tissue: evidence for their involvement in catecholamine-induced luteinizing hormone-releasing hormone release.

ABSTRACT. Medial basal hypothalamic (MBH) and median eminence (ME) fragments from male rats were incubated in Krebs-Ringer bicarbonate-glucose buffer, pH 7.4, at 37 C and the prostaglandin Es (PGEs) released into the medium were measured by RIA. Basal release of PGE by the ME was 5-6 times greater than that by the MBH. However, if the ME was not separated from the MBH, the release of PGE by the MBHME unit was similar to that of the MBH alone. In vivo treatment with indomethacin (Id; [l-(p-chlorobenzoyl)-5-methoxy-2-methylindole 3-acetic acid]) to inhibit PG synthesis (single sc injection, 2.5 mg/100 g BW, 24 h before) decreased the basal release of PGEs by MBH and ME by about 50%. In vitro addition of Id (100 μM) almost completely suppressed basal release of PGEs by both the MBH and ME. Basal release of LHRH by the ME was also significantly depressed by Id. Incubation of the tissues with norepinephrine (NE) at doses (6-600 μM) which increased LHRH output also elicited PGE release. Although dopamine (DA) a...