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Double‐resonance J‐edited 1H‐NMR detection of [6‐13C]‐D‐2‐deoxyglucose uptake in glioma cells

The C6 methylene protons were selectively detected in 1H‐NMR spectra of intact glioma cells incubated with [6‐13C]‐D‐2‐deoxyglucose ([6‐13C]‐2dG), a 13C‐enriched glucose analog that is suitable for monitoring glucose utilization in brain tumors. Spectral editing via 1H–13C scalar coupling was performed with twin spin‐echo double resonance (T‐SEDOR), a pulse sequence which combines chemical specificity and high sensitivity, requires no solvent pre‐saturation, and can easily be adapted to imaging protocols. This work demonstrates the suitability of the pulse sequence for monitoring [6‐13C]‐2dG uptake in living cells in vitro, in spite of line‐broadening and the occurrence of other strong signals in the spectral region of interest (3.5–4.4 ppm). Copyright © 2000 John Wiley & Sons, Ltd.