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Spectral editing technique for the in vitro and in vivo detection of taurine.

In vivo 1H NMR spectroscopy has proven to be a useful noninvasive tool for the investigation of numerous metabolic and physiological states. Taurine is potentially a useful indicator in neonate development and is involved in a number of physiological processes. However, it could not previously be observed in the in vivo 1H spectrum because of overlap with adjacent resonances. We have developed a spectral editing technique based upon double quantum filtration which allows the taurine resonances to be resolved from adjacent peaks. The experiment is demonstrated both on perchloric acid rodent brain extract and on rodent brain homogenate.