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[The in vitro antitumor responses of murine peritoneal macrophages induced by adenovirus-mediated IL-4 and/or M-CSF gene therapy].

OBJECTIVE
To observe the antitumor responses of murine peritoneal macrophages induced by adenovirus-mediated IL-4 and/or M-CSF gene transfer.


METHODS
The IL-4 gene and/or M-CSF gene was transfected into murine peritoneal macrophages-mediated by adenovirus and the levels of IL-4, M-CSF, TNF, IL-1 and NO in the supernatant of the macrophages and the cytotoxicity of the macrophages to tumor cells were assayed.


RESULTS
The high levels of IL-4 and M-CSF could be detected in the supernatant of macrophages 18 hours after being infected with advenovirus expressing IL-4 or M-CSF. The cytotoxicity of the macrophages engineered to secrete IL-4 or M-CSF increased significantly, and when IL-4 gene and M-CSF gene were cotransfected into the macrophages or the macrophages were co-stimulated with LPS, the cytotoxicity increased even more significantly. The levels of TNF, IL-land NO in the supernatant of macrophages also increased.


CONCLUSION
The results demonstrated that transfection of IL-4 and/or M-CSF gene into macrophages could augment their anti-tumor immunity.