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Transient grating of laser-induced 1ΔgO2 production by photodynamic therapy agents

The transient grating method acts as a monitor of the evolution of thermal energy following optical excitation of an absorbing molecule. The signals produced by a photodynamic therapy agent are shown to be strongly dependent on presence of oxygen in solution indicating transfer of energy from a triplet state of the dye to form excited 1Δg oxygen. Analysis of the data shows that the efficiency of excited oxygen production can be determined by a recording of the diffracted light intensity versus time.