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Quantitative image analysis of angle scanning label-free surface plasmon resonance microscopy

In this report, we describe improvement of image resolution in surface plasmon resonance microscopy (SPRM) which suffers from poor quality due to severe surface plasmon (SP) propagation. Our approach takes two-channel momentum sampling by switched light incidence followed by minimum filtering to implement spatially switched SPRM (ssSPRM). The performance evaluated with periodic wires in comparison with conventional SPRM and bright-field microscopy shows that the effect of SP propagation can be circumvented and the effective decay length of SPRM is calculated to increase by only 7% compared to that of bright-field images.