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Stereological Comparison of Imbibed Fibrinogen Gauze versus Simple Gauze in External Packing of Grade IV Liver Injury in Rats

Objective: To evaluate the effect of imbibed fibrinogen gauze on survival, bleeding and healing in liver trauma. Methods: This animal experimental study was conducted on 20 adult male Sprague-Dawley rats; with a mean weight of 300±50 gram; divided into two groups. Grade IV injury was induced to the subjects’ liver. Then, the bleeding site was packed with simple gauze in the control group, and imbibed fibrinogen gauze in the experimental group. All animals were re-evaluated for liver hemostasis 48 hours after the initial injury. Bleeding in the intra peritoneal cavity was measured using Tuberculosis Syringe in the first and second operations. Subjects were followed-up for 14 days. Eventually, the rats were sacrificed and their livers were sent to a lab for stereological assessment. Statistical comparisons were performed via Mann–Whitney U-test using SPSS. P-Values less than 0.05 were considered to be statistically significant. Results: Half of the rats in the control group died, while all the rats in the imbibed fibrinogen gauze group survived after two weeks (p= 0.032). Bleeding in the imbibed fibrinogen gauze was significantly less than control group, 48 hours’ post-surgery (p<0.001). According to the stereological results, granulation tissue in the imbibed fibrinogen gauze group were more than the control group (P= 0.032). Also, fibrosis in the imbibed fibrinogen gauze group were more than the control group (P= 0.014). Conclusion: Our study indicated that imbibed fibrinogen gauze can potentially control liver bleeding and improve survival through increasing granulation tissue and fibrosis in injured liver.


Introduction
B lunt abdominal trauma (BAT), a common form of trauma, is responsible for considerable morbidity and mortality rate [1]. Liver is an intraabdominal organ, frequently injured in blunt and penetrating trauma, and managing its injury has evolved significantly [2]. Although major hepatic trauma (grades IV and V) based on the American Association for the Surgery of Trauma (ASST) grading, is a rare event, even in trauma centers with high patient volume they are associated with a high rate of morbidity and Mortality [3][4][5].
Bleeding is still the leading cause of mortality in patients with liver trauma [6]. Despite advances in damage control studies, minimizing bleeding from parenchymal tissue of the liver is still one of the foremost challenges amongst surgeons while trying to save human lives, especially among patients with higher grade of liver injury and higher Injury Severity Score (ISS) [7]. Massive blood loss are associated with the increased rates of morbidity and mortality, which might be related to blood transfusion or blood products [6]. In other words, massive transfusion along with acidosis and hypothermia might result in coagulopathy, leading to death [8]. In addition to blood loss, the lengthy period that requires to control bleeding is another factor associated with the increased mortality [9, 10]. Developing new ways to control hemorrhage results in introducing numbers of hemostatic dressings and applications that were developed and tested in trauma-relevant animal models. The topical hemostatic agents are frequently used when standard surgical technique are unsatisfactory. Currently, plenty of products are available with moderate success level. Some examples are gelatin, collagen, oxidized regenerated cellulose, fibrin sealant glues, and synthetic glues [11][12][13].
Fibrinogen has been used in surgery since 1940s in which neurosurgeons and plastic surgeons used it for different applications in tissue and nerve repair, wound closure, and skin grafting [14,15]. An intravenous injection form of Fibrinogen supplementation is currently being used as a therapeutic agent for hemostatic management of trauma related bleeding as part of massive transfusion protocol [16]. It is suggested as the initial procoagulant therapy for patients with massive hemorrhage or in cases that significant bleeding is accompanied by signs of fibrinogen deficiency [17]. To the best of our knowledge, no study has ever evaluated or compared the efficacy of external usage of fibrinogen on liver injury. We aimed to compare intraoperative and postoperative findings on rats that were packed with imbibed fibrinogen gauze after inducing grade IV injury.

Ethical Approval
The study protocol was approved by the local ethics committee of Shiraz University of Medical Sciences, Shiraz, Iran (No IR.SUMS.MED.REC.1396.s146), and in accordance with the international conventions on animal experimentation. All procedures were performed under general anesthesia. We did our best to minimize the animals' suffering during the experiment. The animals received care in which they were kept in separate clean wire-bottomed cages. Their environment was temperature controlled (22°C ± 2°C) and humidity controlled (55% ± 15%), with 12hrs light/dark photo cycles. They had free access to equal amounts of standard rodent chow and water. They were allowed to adapt to their environment for one week prior to the experiment. Before laparotomy, the rats were anaesthetized with intramuscular injection of ketamine (50 mg/kg; Alfasan International, Woerden, the Netherlands), and xylazine (10 mg/kg; Alfasan International). Anesthesia lasted about 20-30 minutes. After inducing laceration, we administrated warm isotonic saline (<10ml/kg) for resuscitation [18]. To control hypothermia, we used rectal temperature (Tr) and the temperature was maintained at 38.0°C±0.5°C with a heating lamp and resuscitation with warm isotonic saline [19,20].

Animals and Hepatic Injury Model
After consulting with a biostatistician to determine the sample size, a total of 20 healthy adult male Sprague-Dawley rats (with a mean weight of 300±50 gram) were used in this study. The rats were stratified into two groups using a simple randomization method (10 rats per each group); group A: those for which simple gauze was applied to their liver laceration (control group); group B: rats for which imbibed fibrinogen gauze was applied on the liver laceration (case group). Fibrinogen) Haemocomplettan, CSL Behring GmbH, 35041 Marburg, Germany) was applied via 70mg/kg. Before laparotomy, the rats were anaesthetized with intramuscular injection of ketamine (50 mg/ kg), and xylazine (10 mg/kg). We initiated the surgical procedure after anesthesia. After shaving the abdomen, the incision site was disinfected using alcohol ethylic solution. In the next step, with surgical knife, a vertical incision was made starting from the xiphoid process at about three centimeters long. After opening the abdominal wall, we placed a small orthostatic retractor and identified the liver. After that standardized trauma with knife (20mm in diameter and 5mm in depth) was done in the parenchyma of the major (middle) lobe of the liver and this part of liver was removed by clamp ( Figure 1). All surgical procedures were performed by one surgeon in order to minimize the bias. After 2 minutes of uncontrolled bleeding, the bleeding in the intra peritoneal cavity was measured using Tuberculosis Syringe. Then, the packing applied on the injured liver was done according to the group they belonged to. After 15 minutes, the intra peritoneal bleeding was reassessed. Meanwhile, intra peritoneal resuscitation was done with normal saline. Also, the packing remained in both groups after the first operation.
Then, fascia and skin were sutured with PDS 3.0 string. After surgery, the animals were kept in their cages under the care of a veterinarian and standard care including food and water.
After 48 hours, laparotomy was performed again (second look), and hemostasis and the need for repacking were evaluated. To do so, we removed hemostatic agent and gauze to see whether the bleeding had stopped. In the case, which bleeding still remained, we stopped the bleeding by it packing with finger and cauterization. Finally, we closed the abdomen. Also, during the second look, we measured the intra peritoneal bleeding for the third time. From the first day of first operation, we followed the subjects for 14 days. Eventually, we sacrificed the rats and sent their liver for pathology assessment via stereological method. All the specimens were fixed via formalin. The variables collected for the study, were the intra peritoneal bleeding 2 min, 15min and 48hrs after laparotomy, the occurrence of deaths and any histological changes (assessed by stereology method via an operator who was blind to the groups allocation).

Histopathological Evaluation of Liver Injury (Stereology)
After 14 days, to determine the effect of fibrinogen on healing of the injured liver, we measured the volume and weight of lacerated major-liver lobe. We also measured the normal liver tissue, granulation tissue, amorphous tissue and fibrosis with proportion of lacerated area to total area of liver (via measuring volume density). Livers were fixed in neutral buffered formaldehyde for at least one week. The major middle lobe of liver was separated and the weight and volume measured (according to the immersion method) [21][22][23][24]. Coronal sections with equal distances were made through the entire context of major lobe. Then, 10-12 sections for each part of major lobe were sampled through systemic uniform random sampling. The sampled sections were then processed and embedded in the same paraffin block. Four micrometer sections were prepared and stained using Heidenhain's azan trichrome and hematoxylin and eosin.

Estimating the Volume of the Normal Liver Tissue, Granulation Tissue, Amorphous Tissue and Fibrosis
The microscopic evaluations were done using a computerized video-microscopy system. The stereological counting equipment consisted of a Nikon E-200 microscope (Nikon, Japan) with a motorized stage linked to a computer. To perform stereological counting, the stereological grid of points was generated using a software designed at Shiraz University of Medical Science, Shiraz, Iran, and stereological probes (point grids and counting frames) were superimposed onto the live images of each section. Volume density (Vv) refers to fraction of the unit volume of tissues occupied by the structure of interest [21][22][23][24]. The volume density of normal liver tissue, granulation tissue, amorphous tissue, and fibrosis (the fraction of unit volume of the liver occupied by normal liver tissue, granulation tissue, amorphous tissue and fibrosis ) were estimated using the point counting method [24][25][26][27]. Briefly, a grid of points was superimposed on the liver image sections, viewed on the monitor at a final magnification of 180. The density was computed according to the following formula: Vv (structure, reference)=P (structure)/P (reference) where "P(structure)" and "P(ref)" represent the total number of the points hitting the structures of interest (normal liver tissue, granulation tissue, amorphous tissue and fibrosis) and the total number of points laid on the profiles of the liver section, respectively ( Figure 2) [28][29][30].

Statistical Analysis
Statistical analysis was done by a statistician who was blinded to the study. The results are shown as mean ± standard deviation (SD). Statistical comparisons were performed via Mann-Whitney U-test (version 22; SPSS Statistics software, Chicago, IL). P-Values less than 0.05 were considered to be statistically significant. The results are shown as standard dot plots. Additionally, power analysis was done to find the power of study via Stata software, version 11.2 (Stata Corporation, college station, TX, USA).

Bleeding
There was no statistically significant difference among intra peritoneal bleeding between group A and B 2 min and 15 min post-liver surgery. However, there was a significant difference between group A and B in intra peritoneal bleeding 48hrs post-liver injury (Table 1).

Need for Repacking After 48 hours
In total, 30% (3/10) of rats in gauze packed group required repacking after 48hrs. Their bleeding was controlled with finger and cauterization (As explained in the method).

Mortality
While 50% (5/10) of the rats in group A died, 100% of rats in group B survived after 14 days, which was statistically significant (p=0.032). All 5 rats in group A died after 48hrs (second laparotomy).

Stereological Results
As shown in Figure 2, there was no significant difference in weight and volume of major lobe between the two groups. Also, volume density of fibrosis, granulation tissue, and amorphous tissue in group B was significantly more than group A (Figures 2 and 3). In contrast, normal liver tissue in group B was less than group A (Figures 2 and  3). Table 2 shows the result. The mean power of our study was 94.5% (the lowest=85%).

Discussion
Fibrinogen, the final component of the coagulation pathway, is used in major bleeding [31]. Two main Fig. 2. The dot plots of the difference among weight (A) and volume (B) of major liver lobe between the control (gauze packed) and imbibed Fibrinogen gauze groups are shown. Also, dot plots showed volume density of the normal liver lobe (C), granulation tissue (D), amorphous tissue (E), and fibrosis (F) in the injured site of the major liver lobe in the two groups. The differences among the groups is indicated on the plots. sources of fibrinogen are currently available in clinical applications; cryoprecipitate and fibrinogen concentrates. Fibrinogen concentrate is easier to store, administer, and safer than cryoprecipitate; however, it is more expensive [17]. Today, it is used in massive transfusion in case of critical bleeding [16]. As a hemostatic agent, it is used in combination with other agents. Frederico Michelino et al. used collagen-based adhesive associated with fibrinogen and thrombin in experimental liver injury in Wistar rats, which resulted in controlling hemorrhage with little adhesion [32]. We used pure fibrinogen concentrate and chose simple gauze as a basis for applying it on the injured liver.
Despite the small sample size, we were able to show a trend toward lower blood loss and better healing as well as increasing survival rate after liver packing.
Liver is a common organ injured following abdominal trauma [2,33], and its injury is a major obstacle in providing successful treatment due to frequency, location and size. Delay in controlling hemorrhage has negative impact on the outcome, leading to high morbidity and mortality [34,35]. Surgical techniques, such as manual pressure and thermal methods like electrocauterization might lead to scar and necrotic tissue as well as increasing the probability of infection [36,37]. Additionally, conventional methods are less effective in controlling bleeding in complex injuries, especially when there is difficulty accessing the injured area [37,38]. Despite numerous endeavors to reduce the related mortality, but it is still inevitable. Mortality rate was reported 30% in the management of complex liver injury with resection [39] and 66% following grade IV and V liver trauma [40]. Furthermore, cirrhosis-related coagulopathy, blood loss and prolonged surgery might lead to a vicious circle of acidosis, hypothermia and coagulopathy (lethal triad). Hence, finding a new method might help to stop this cycle [41,42]. According to aforementioned reasons and difficulties, topical homeostatic agents might be particularly useful in such situations.
Until now, several agents have been identified to be applied on injured liver. Hemostatic agents, such as surgicell, thrombin-soaked gel foam, or fibrin glue are useful adjuncts [43]. However, these favorable agents have their own complications like bile leakage and rebleeding [44,45]. Moreover, Banihashemi et al. showed the effectiveness of fibrin packing on stab wounds of the liver, which resulted in the survival of all injured rats [46]. Fibrinogen molecules have two sets of disulfide-bridged Aa-, Bb-, and c-chains. Each molecule has two outer D domains connected to a central E domain by a coiled-coil segment. Fibrin is formed after cleavage of fibrinopeptide A (FPA) from fibrinogen Aa-chains via thrombin, resulting in initiating fibrin polymerization [47]. In addition, Rosselli et al. investigated the use of topical bovinederived thrombin solution as a hemostatic agent in a rodent model of hepatic injury and found this agent to be insufficient in controlling injured liver bleeding [48]. Samokhvalov et al. induced grade IV liver laceration in rats and packed it with Celox. They found Celox 100% to be effective in controlling liver bleeding [12]. Another study showed a fully synthetic, polyurethane based glue (MAR-1) as a suitable agent in controlling the bleeding in 50% resection of the lateral left liver lobe in male Wistar rats [49].
Perhepatic gauze packing (PHGP) is an acceptable  method in damage control laparotomy for packing the abdominal organs to control bleeding [50,51]. PHGP became more important, especially when it was compared with controlling bleeding via liver resection that had high mortality and some complications [42,[52][53][54], but PHGP has its own complications. It sometimes fails to control hepatic hemorrhage and is often associated with massive transfusion and frequent development of abdominal compartment syndrome [55,56]. In our study, intraperitoneal bleeding in gauze packed group was more than the imbibed fibrinogen gauze group 48hrs after injury. Three subjects in the gauze packed group required repacking after second look, and eventually half of the rats in this group died in the days after operation.
All of our rats in the imbibed fibrinogen gauze group survived after 14 days of follow-up. Measurement of bleeding 15 minutes and 48hrs after operation was lower in comparison to simple gauze packed group. Therefore, fibrinogen had a better impact on controlling hemorrhage at the time of operation and the subsequent days after operation. Interestingly, our imbibed fibrinogen-gauze group show no need for repacking. Moreover, volume density of granulation tissue and fibrosis were more than our control group. Therefore, our rats experienced better healing during the 14 days of follow-up, which resulted in 100% survival. As far as we know, fibrinogen has not been used for external packing of liver in any other study. Thus, this study is unique in introducing new agent to control bleeding in trauma of the liver, opening a new horizon for it to be used in liver injury in humans. One of our limitations was the second look for the evaluation of hemostasis. Although fibrinogen showed its efficacy in controlling the bleeding, second look while using gauze was inevitable. Hence, finding a proper base for applying fibrinogen on the liver is still a challenging matter. Another limitation was that we did not measure bile leakage during the days following operation, but we assumed that due to more fibrosis in the rats packed with imbibed fibrinogen gauze group, lesser bile leakage might had occurred in comparison to gauze packed group. To conclude, this study showed that imbibed fibrinogen gauze resulted in no mortality, significant fibrosis and granulation tissue on injured area and efficient hemorrhage control. Thus, treatment with imbibed fibrinogen gauze is potentially effective in experimental liver trauma.