{'fraction_non_alphanumeric': 0.046910495229441164, 'fraction_numerical': 0.02277373920945025, 'mean_word_length': 5.030479452054794, 'pattern_counts': {'":': 0, '<': 6, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Changes in the Neuronal Architecture of the Hippocampus in a 6-Hydroxydopamine-Lesioned Rat Model of Parkinson Disease

rats. Both motor and nonmotor symptoms alongside the expression of tyrosine hydroxylase (TH) in the substantia nigra and striatum were confirmed in 6-OHDA-lesioned rats. The neuronal architecture in the hippocampus was analyzed by Golgi staining. Results: During the 7–8 weeks after infusion, the 6-OHDA-lesioned rats exhibited motor and nonmotor dysfunctions (espe-cially anxiety/depression-like behaviors). Rats with unilateral 6-OHDA infusion displayed reduced TH+ immunoreactivity in the ipsilateral nigrostriatal pathway of the brain. Golgi staining revealed that striatal 6-OHDA infusion significantly decreased the dendritic complexity (i.e., number of crossing dendrites, total dendritic length, and branch points) in the ipsilateral hippocampal conus ammonis 1 (CA1) apical/basal and dentate gyrus (DG) subregions. Additionally, the dendritic spine density and morphology were significantly altered in the CA1 apical/basal and DG subregions following striatal 6-OHDA infusion. However, alteration of microglial and astrocytic distributions did not occur in the hippocampus following striatal 6-OHDA infusion. Conclusions: The present study provides anatomical evidence that the structural plasticity in the hippocampus is altered in the late phase following striatal 6-OHDA infusion in rats, possibly as a result of the prolonged suppression of the DAergic system, and independent of neuroinflammation.


INTRODUCTION
Parkinson disease (PD) is the world's second most common neurodegenerative disorder [1]. It is distinguished by a significant loss of dopaminergic (DAergic) neurons, primarily in the substantia nigra (SN), and results in a decrease in DAergic fibers in the striatum [2]. Additionally, PD is a neurological disease that causes both motor and nonmotor symptoms [3]. The main clinical focus in PD has been on motor symptoms; nevertheless, there is growing acknowledgment that the clinical spectrum of PD is more comprehensive, and includes nonmotor functions [4]. The most prevalent nonmotor symptoms in PD are depression, anxiety, cognitive decline, pain, fatigue, insomnia, and autonomic dysfunction. Furthermore, approximately 40%-50% of PD patients are diagnosed with anxiety or depression [5]. Moreover, the nonmotor symptoms that are associated with the hippocampus significantly affect PD patients' quality of life [6].
Since DAergic damage is a defining characteristic of PD, the structural, molecular, and functional alterations in the nigrostriatal system have garnered much attention [7]. However, research on the other brain regions in PD is needed to clarify their roles, their interaction with the nigrostriatal system, and their involvement in the mechanism underlying nonmotor dysfunctions. The hippocampus is critical for cognitive and emotional regulation and receives DAergic projections from the ventral tegmental area and SN, respectively [8]. Dopamine (DA) modulates hippocampal long-term potentiation (LTP) [9]. In PD, the DAergic system interacts with the synaptic plasticity of the hippocampus [10], and the hippocampus is implicated in the nonmotor dysfunctions of PD [8]. In preclinical PD studies, synaptic mechanisms underlying hippocampal dysfunction were reported in neurotoxic and genetic animal models of PD, including 6-hydroxydopamine (6-OHDA)-lesioned rats [11] and α-synuclein transgenic mice [12], respectively.
Neuroplasticity refers to the capacity of neuronal networks in the brain that change through development and reorganization; it is typically classified as structural and functional plasticity [13,14]. Structural plasticity, such as dendritic formation and spine development, is controlled by the regulation of the neuronal actin cytoskeleton [15]. Neurodegenerative diseases may be caused by dendritic and spine alterations produced by acute or chronic disruptions in brain tissue homeostasis [16]. Structural plasticity in the hippocampus can be influenced by a variety of stimuli, some of which appear to have long-term effects [8]. Previous studies found that dendritic complexity and/or spine density was altered in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-lesioned [17,18] and leucine-rich repeat kinase 2-mutant mouse PD models [19,20]. However, many questions remain unanswered about the functional and structural changes of hippocampal neurons that occur in PD. In this study, a hemiparkinsonian rat model with an ipsilaterally striatal infusion of 6-OHDA was used, which mimics features of the human pathological condition of PD [21]. Therefore, this study attempted to analyze behavioral impairments and alteration of structural plasticity in the hippocampus, as well as explain their correlations in this animal model.

Animals and Surgical Procedure
Male Sprague Dawley rats weighing 250-300 g were obtained from Charles River Laboratories (Wilmington, MA, USA) at 8 weeks old and allowed to acclimate for 1 week prior to use in the study. The rats were immobilized in a stereotaxic device (SR-6; NARISHIGE, Tokyo, Japan) with a flat head position after anesthesia. The coordinates were as follows: anteroposterior, +1.3, +0.4, -0.4, -1.3 mm from the bregma; mediolateral, -2.6, -3.0, -4.2, -4.5 mm from the midline; and dorsoventral, -5.0, respectively, from the skull. Per rat: the 6-OHDA (3.5 mg/mL, Sigma-Aldrich, St. Louis, MO, USA) was administered into the right striatum with an infusion pump using an 8 µl saline with 0.02% (w/v) ascorbic acid (Wako, Osaka, Japan) through a 10.5-μL microinjection cannula at a continuous flow rate of 1 μL/ min (2 μL was injected at each coordinate). Sham-operated rats were subjected to the identical method, except instead of 6-OHDA, 8 μL of the vehicle (0.9% saline containing 0.02% (w/ v) ascorbic acid) was injected into the striatum.
-Structural plasticity is altered in the hippocampus of 6-OHDA-lesioned rats.
-Hippocampal dysfunctions may be related to the alteration of structural plasticity in Parkinson disease animal models, possibly by a decrease in DAergic signaling.

Apomorphine-induced rotation test
Four weeks after striatal 6-OHDA infusion, apomorphine hydrochloride (1 mg/kg; Wako) was given intraperitoneally into the rats. The number of left-handed (contralateral) rotations was monitored for 30 minutes. Hemiparkinsonian rat models for PD were found to have a rotational velocity of more than 7 turns/min.

Cylinder test
The test was performed as follows: individual rats were placed within a glass cylinder (diameter, 22 cm; height, 26 cm) with one mirror set below the cylinder at a 45° angle to provide 360° viewing. The rats were videotaped for 5 minutes following initial contact with the walls of the cylinder with their impaired, unimpaired, or both forelimbs. The scores were computed using the asymmetry ratio, left-right/(right + left + both). The forelimb asymmetry ratio scores vary from 1 to 1. The positive ratio indicates that the unimpaired forelimb is utilized more than the impaired forelimb. The negative asymmetry ratio shows that the impaired forelimb is used more than the unimpaired forelimb.

Object exploration test
In the center of the open box (W × D × H: 60 × 60 × 30 cm), a cylinder item (diameter, 5.5 cm; height, 13 cm) was exposed. A rat was placed in the open box with its back to the test object and given 10 minutes to explore. The time it took for a rat to approach an object and the duration of each subsequent approach were recorded.

Light/dark box test
Rats were examined in 2 compartments (W × D × H: 60 × 30 × 30 cm), which were connected together length-wise. There was a 10 × 10 cm square aperture between the 2 compartments that the rat could utilize and move between. One chamber was closed with a lid (i.e., the dark box), whereas the other was white, left uncovered, and illuminated with an 880-lux light (i.e., the light box). Again, the rat was allowed 10 minutes to explore both chambers. Here, both the frequency of entry and the duration spent in the light box were calculated.

Forced swim test
This test exposed the rats to a cylindrical container (diameter, 20 cm; height, 70 cm) containing water at a temperature of 24°C ± 1°C and a depth of 30 cm. The duration of time the rat floated upright without moving or 'struggling' -aside from efforts to maintain its head above water-was known as the time spent in immobility.

Immunohistochemistry
All immunohistochemistry methods were followed as described in previous studies [22,23]. The fixed brain hemispheres were sectioned in the coronal plane to a 30-µm thickness. To inactivate endogenous peroxidase activity, free-floating sections were incubated in 0.3% (v/v) hydrogen peroxide for 20 minutes before being blocked with 5% (v/v) normal goat serum (Vector ABC Elite Kit; Vector Laboratories, Burlingame, CA, USA) in 0.3% (v/v) Triton X-100 for 1 hour at room temperature (RT; 22°C±2°C). Sections were then incubated overnight at 4°C with rabbit anti-tyrosine hydroxylase (TH; 1:500; Millipore, Darmstadt, Germany), rabbit anti-glial fibrillary acidic protein (GFAP; 1:2,000; Dako, Glostrup, Denmark), or the rabbit anti-ionized calcium-binding adapter molecule 1 (Iba1; 1:1,000; Wako, Osaka, Japan). Sections were incubated for 1 hour at RT with biotinylated goat anti-rabbit IgG (Vector ABC Elite Kit), and then incubated for 1 hour at RT with an avidin-biotin-peroxidase complex (Vector ABC Elite Kit). The peroxidase reaction was induced using a diaminobenzidine substrate (DAB kit; Vector Laboratories). Three hemisections of the SN (approximately 5.08 mm caudally from the bregma) were selected from each rat for cell counting. The mean number of immunopositive cells in the 3 sections of each rat was taken as n = 1. Moreover, the images were converted to grayscale to measure TH immunoreactivity in the striatum (approximately 0.26 mm caudally from the bregma) alongside GFAP and Iba1 immunoreactivities in the hippocampus (approximately 3.80 mm caudally from the bregma). The mean gray value (256 gray levels) was calculated using ImageJ software (NIH, Bethesda, MD, USA) for each chosen area. The relative changes in intensity levels of TH+, GFAP+, and Iba1+ immunoreactivities were expressed as relative to their optical densities (ODs) after setting the mean intensity of the control to 1. The number and intensity of the immunopositive cells per group were averaged and expressed as mean ± standard error (SE; n = 3 rats/group).

Western Blotting
All Western blot protocols were followed as described in previous studies [22,23]. Electrophoresis was used to separate proteins on 7%-15% sodium dodecyl sulfate polyacrylamide gel. The proteins were subsequently transferred to polyvinylidene difluoride membranes. The membranes were then blocked for 1 hour at RT with 1% (v/v) bovine serum albumin (Sigma-Aldrich) and 2% (v/v) NGS (Vector Laboratories) in phosphatebuffered saline (PBS) containing 0.1% (v/v) Tween 20 (PBS-T; pH 7.4). The membranes were subsequently treated overnight at 4°C with rabbit anti-GFAP (1:2,000) and Iba1 (1:1,000) pri-mary antibodies. The membranes were incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (Vector Laboratories) for 1 hour at RT, and the signals were detected using a chemiluminescence kit (SuperSignal West Pico; Thermo Fisher Scientific, Waltham, MA, USA). After stripping, the membranes were probed again with the mouse anti-β-actin antibody (1:5,000; Sigma-Aldrich) for 2 hours at RT. The OD of each band was assessed using an iBright CL750 Imaging System (Thermo Fisher Scientific).

Golgi Staining
The dendritic complexity, spine density, and spine morphology of the neurons in the hippocampal CA1 and DG subregions were studied using Golgi impregnation. The FD Rapid Golgistain Kit and accompanying techniques were employed in accordance with the manufacturer's guidelines (FD Neurotechnologies, Ellicott City, MD, USA).

Sholl Analysis
As previously reported [22,23], both CA1 and DG neurons of the hippocampus were traced and analyzed. Using a camera lucida at a magnification of 200 ×, 10 neurons from each hippocampal region were randomly dispersed among 3 brain slices (approximately 3.80 mm behind bregma) in each animal. Sholl' s concentric circle method [22,23] was used to objectively examine the dendrites for each of the chosen neurons. Dendrites intersecting each circle were counted to determine the number of dendritic intersections at different radial distances from the neuronal soma, in addition to the total dendritic length, and branch points. Each value was averaged per rat, and the mean value of each rat was taken as n =1. The value per group was averaged and expressed as mean±SE (n=8 rats/group).

Measurement of Spine Density and Morphology
All discernible projecting dendritic spines were counted along 30-µm dendritic segments from the terminal to the tip of 3 dendrites from each neuron using 1,500 × magnification. Spines were divided into the following morphological categories: (1) thin: spines with a discernible small head and an extended neck, (2) mushroom: spines with a voluminous head and a noticeable neck, and (3) stubby: spines with an overall stubby look and no discernible neck. Ten segments were counted in each animal (n=8 rats/group), and the spine density was determined as the number of spines per 10 µm of dendritic length.

Statistical Analysis
All statistical analyses were performed using Prism (GraphPad Software, San Diego, CA, USA; RRID: SCR_002798). The changes between the number of dendritic intersections at different radial distances of the sham-operated controls and 6-OHDA-lesioned rats were identified, for 8 rats per group, using two-way repeated-measures analysis of variance followed by multiple comparison tests corrected with Šidăk's post hoc test. Unpaired Student t-tests were used for all other analyses to compare the means of the sham-operated controls and 6-OH-DA-lesioned rats. For all statistical tests, a P-value < 0.05 was considered statistically significant.

6-OHDA-Lesioned Rats Displayed Significant Reduction in Dendritic Arborization of Hippocampal Neurons in the CA1 and DG Subregions
Eight weeks following the striatal 6-OHDA infusion, Golgi staining was used to track and quantify the dendritic complexity of the ipsilateral CA1 and DG neurons. The number of dendritic intersections was counted at various radial distances from the neuronal soma using Sholl analysis (Figs. 3A, 4A, n = 8 rats/ group). At Sholl radii of 30-170 µm from the soma, the CA1 apical dendrites in the hippocampi of 6-OHDA-lesioned rats had significantly fewer intersections than those in sham-operated controls (Fig. 3B, left panel; Finteraction [34, 2652] = 10.44, P < 0.0001). The number of branching points in the CA1 apical dendrites in 6-OHDA-lesioned rats was significantly lower than in sham-operated controls (Fig. 3B, middle panel; t(14) = 8.317, P < 0.0001). Moreover, striatal 6-OHDA infusion significantly reduced the total length of the CA1 apical dendrite in the hippocampi of rats (Fig. 3B, right panel: t(14) = 3.394, P < 0.01). In

Striatal 6-OHDA Infusion Significantly Altered Dendritic Spine Density and Morphology in the Hippocampal CA1 and DG Subregions
The Golgi-stained neurons were magnified and their dendritic spine density and morphology were analyzed for any alterations (Fig. 5A-C, left panels). The dendritic spine densities (the number of spines per 10-μm dendrite) were significantly decreased in the apical CA1 (Fig. 5A,  . Bar graphs display the spine density per 10 µm of the dendrite (A-C, middle panels) and spine morphology per 10 µm of the dendrite (A-C, right panels) in each subregion. Data are expressed as the mean ± standard error (n = 8 rats/group). 6-OHDA, 6-hydroxydopamine-lesioned group; Sham, sham-operated controls. *P < 0.05. **P < 0.01.

DISCUSSION
In this study, unilaterally striatal 6-OHDA infusion produced persistent motor (hypolocomotion and asymmetric behavioral deficit) and nonmotor (anxiety/depression-like behaviors) symptoms. Additionally, it induced the destruction of DAergic signaling in the ipsilateral nigrostriatal pathway of rats; identified through the loss of TH+ neurons in the SN and TH+ fibers in the striatum. Intriguingly, the striatal 6-OHDA infusion significantly altered neuronal architecture (dendritic complexity alongside spine density and morphology) in the ipsilateral hippocampus and independently of neuroinflammation.
Pathological characteristics of PD include nigrostriatal DAergic degradation, which affects motor function [24]. Asymmetries in sensorimotor reactivity beginning 6 weeks [25] and impairments in motor coordination and balance abilities at 2 weeks [26] are the most noticeable deficiencies observed in unilateral 6-OHDA-infused rats. Indeed, in this study, rats with striatal 6-OHDA infusion were subjected to a complete evaluation of PD-like behaviors utilizing multiple behavioral paradigms for assessing motor and nonmotor symptoms. Similar to previous studies [27], the present study uncovered noteworthy anxiety-and depression-like behaviors in rats infused unilaterally with 6-OHDA. Therefore, we found that striatal 6-OHDA infusion generated both motor and nonmotor dysfunctions, including anxiety/depression-like behaviors, which are associated with hippocampal dysfunctions.
DA affects hippocampal LTP [9] along with hippocampusdependent learning and memory [28]. The hippocampus is implicated in the nonmotor dysfunctions of PD [8], and hippo-