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[Effect of the addition of DMSO and EDTA on the activity of formaldehyde and hydrogen peroxide against Bacillus cereus spores].

The question was investigated if DMSO in concentrations between 1 and 6% and 0.1% EDTA could improve the effect of the sporocidal disinfectants formaldehyde and hydrogen-peroxide. In suspension-tests with spores of Bac. cereus it was found, that the sporocidal activity of a 1.2 and 3% formaldehyde solution did not increase, in contrary it decreased significantly if EDTA or DMSO were present in the solution. The same effect could be found with 0.8-5% hydrogenperoxide in combination with the above mentioned substances. These results are not in agreement with the observations of other authors working with vegetative bacteria. It is supposed that DMSO rather reactivates injured spores than that it acts as a carrier for the respective disinfectant. This effect seems to mask a relatively faint increase of the permeability for the disinfectants. Concerning EDTA it could be possible, that it did not succeed in removing the Ca++ from the cortex, in contrary it seems, that it binds itself to the calciumdipicolinate thus stabilizing the spore structure.