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Biological component microanalysis by laser photoacoustic imaging immunoassay.

Computerized laser photoacoustic microscopy was developed and applied to the immuno-selective imaging of the microdistribution of a biological component. Human lambda-type light-chain was chosen as a model target protein and its peroxidase conjugated antibody was used for selective binding to the target protein and its staining. Using a microscopic calibration curve which was obtained from the images of homogeneously dispersed standard samples, the total quantities of randomly dispersed artificial standard samples were estimated to evaluate the reliability of this method. Finally, the method was applied to a tissue section sample, a human fetal pancreas section, to get the local and total quantitative images of the lambda-type light-chain.