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[Analysis of corticosteroids in blood plasma using a microcolumn liquid chromatography method].

A microcolumn variant of high-pressure liquid chromatography of human blood plasma corticosteroids using Milikhrom, a Soviet chromatograph, is suggested. The analysis involves hexane treatment of the plasma, chloroform extraction of corticosteroids, concentration of the extract, and chromatography in a 60 X 2 mm column packed with Silasorb sorbent, sph C18 (7.5 micros), methyl alcohol/water mixture (52:48) at a rate of 100 microliter/min and a detection wavelength 240 nm. The method permits an analysis of cortisone, hydrocortisone, corticosterone, and other corticosteroids with concentrations higher than 4 ng/ml.