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Particulate Bioluininescence in Dinoflagellates: Dissociation and Partial Reconstitution

With the same extraction conditions used for Gonyaulax polyedra, soluble and particulate bioluminescence can be isolated from two additional species, Pyrodinium bahamense and Pyrocystis lunula. We have been able, for all three species, to dissociate soluble luciferin and luciferase from the particulate system. Luciferin can be incorporated into both reacted and unreacted particulate systems.