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LOCALIZATION OF HEXOKINASE IN NEURAL TISSUE: LIGHT MICROSCOPIC STUDIES WITH IMMUNOFLUORESCENCE AND HISTOCHEMICAL PROCEDURES

Abstract— The distribution of hexokinase (ATP: D‐hexose 6‐phosphotransferase, EC 2.7.1.1) in rat cerebellum, retina, hippocampus, choroid plexus and ependymal cells of the cerebral ventricles, and dorsal root ganglion has been determined, at the light microscopic level, by both immunofluorescence and a histochemical procedure using nitro blue tetrazolium. With the exception of an artifactual staining of the outer photoreceptor segments of retina when the histochemical procedure was used, both methods gave comparable results, from which the following conclusions are drawn: