{'fraction_non_alphanumeric': 0.03397027600849257, 'fraction_numerical': 0.028662420382165606, 'mean_word_length': 4.8, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Dopamine D 1 Receptor and Protein Kinase C Isoforms in Spontaneously Hypertensive Rats

Dopamine, via D1-like receptors, stimulates the activity of both protein kinase A (PKA) and protein kinase C (PKC), which results in inhibition of renal sodium transport. Since D 1-like receptors differentially regulate sodium transport in normotensive and hypertensive rats, they may also differentially regulate PKC expression in these rat strains. Thus, 2 different D1-like agonists (fenoldopam or SKF 38393) were infused into the renal artery of anesthetized normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) (n 55 to 6/drug/strain). Ten or 60 minutes after starting the D 1-like agonist infusion, both the infused kidney and the noninfused kidney that served as control were prepared for analysis. The D 1-like agonists produced a greater diuresis and natriuresis and inhibited Na,K-ATPase activity in proximal tubule (PT) and medullary thick ascending limb (mTAL) to a greater extent in WKY (D2061%) than in SHR ( D761%,P,0.001). D1-like agonists had no effect on PKCa or PKC-l expression in either membrane or cytosol but increased PKCu expression in PT in both WKY and SHR at 10 minutes but not at 60 minutes. However, membranous PKCd expression in PT and mTAL decreased in WKY but increased in SHR with either 10 or 60 minutes of D 1-like agonist infusion. D1-like agonists also decreased membranous PKCz expression in PT and mTAL in WKY but increased it in PT but not in mTAL in SHR. We conclude that there is differential regulation of PKC isoform expression by D 1-like agonists that inhibits membranous PKCd and PKCz in WKY but stimulates them in SHR; this effect in SHR is similar to the stimulatory effect of norepinephrine and angiotensin II and may be a mechanism for their differential effects on sodium transport. (Hypertension. 1998;32:1049-1053.)