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Immobilization of Polydiacetylene Sensor on Solid Substrate

Immobilized polydiacetylene vesicles on solid substrates were successfully prepared by utilizing imine formation or Diels-Alder reaction. Incubation of an aldehyde-modified glass substrate in an aqueous solution containing self-assembled diacetylene vesicles allowed efficient formation of immobilized polymer vesicles. In addition, preparation of immobilized polydiacetylene vesicles on solid substrate was achieved, for the first time, using the Diels-Alder reaction with diacetylene monomers having maleimido headgroups and a furan-modified glass substrate. Patterned fluorescent images were obtained with immobilized vesicles by selsctive irradiation through a photomask followed by heating the glass substrate at 100 o C.