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On cultivation of the alga Vaucheria dichotoma (Xanthophyceae) in axenic culture

Abstract The alga Vaucheria dichotoma was isolated and cultivated under axenic conditions. Optima of various parameters were determined: temperature 22–23 °C, pH 8.0–8.5, salinity around 1%. As a P-source, α- or β-glycerophosphate was as good as inorganic phosphate; good growth was obtained within a wide range of concentrations of all three P-compounds. NO3− gave the best yield from six different N-sources tested and it was tolerated in very high concentrations; the organic N-source did not affect growth at all. C-sources, like glucose, sodium acetate or glycolic acid, did not change the yield, either in the dark or at two different light intensities. Vitamin B12 was required or greatly stimulating to growth depending on the age of the culture used as inoculum.