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Glyceollins Attenuates AOM/DSS‐induced Colorectal Cancer Development in C57BL/6 Mice

Glyceollins are soybean phytoalexin possessing anti‐inflammatory and anti‐carcinogenic activities. In this study, we examined if glyceollin administration suppresses cancer development using an inflammatory colorectal cancer animal model, azoxymethane/dextran sodium sulfate (AOM/DSS)‐treated C57BL/6 mice. To enrich glyceollins, soybean seeds were inoculated with Aspergillus sojae. After 7 day‐incubation, the soybeans were extracted in 80% ethanol. Glyceollins were subsequently purified by liquid chromatography from the extract. The prepared glyceollins were orally administered to AOM/DSS‐treated mice, followed by morphological, histological and biochemical analyses on colon tissue samples. The number and size of colon polyps were measured to assess the development of colonic tumorigenesis. Histological examination on the colon samples and polyps was performed by hematoxylin and eosin staining. Blood C‐reactive protein level and neoplasia‐associated protein levels were also analyzed. Our findings exhibited that glyceollins effectively reduced colorectal cancer development following AOM/DSS treatment, implicating the nutraceutical and cancer‐preventive potential of soybean‐derived glyceollins in colitis‐colorectal cancer.