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Photo-induced delayed luminescence of human serum and its dependence on excitation conditions

The photo-induced delayed luminescence (DL) of human serum and its dependence on exciting conditions, including exciting wavelength, exciting energy and exciting power, were studied in this paper. It was found that the DL of serum follows the law of hyperbolic decay rather than exponential decay, exhibiting coherent character. The exciting conditions had affinities with the activation as well as the active reactions of biological molecules, which were sensitive and active under UV-light excitation. Exciting energy mainly decides the activation. More sufficient activation leads to more drastic active reactions and stronger re-emission ability of bio-molecules after illumination, resulting in the more intensive photon emission and lower DL decay speed rate. On the other hand, exciting power also plays an important role in impacting the active reactions. Exciting light with higher power makes the active reactions more drastic, causing the higher photon counts. However, there are few correlations between exciting power and the re-emission ability of bio-molecules. These results may be useful for investigation and application of human serum.