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Molecular vibrational imaging by stimulated Raman scattering microscopy: principles and applications [Invited]

The recent development of stimulated Raman scattering (SRS) microscopy allows for highly sensitive biological imaging with molecular vibrational contrast, opening up a variety of applications including label-free imaging, metabolic imaging, and super-multiplex imaging. This paper introduces the principle of SRS microscopy and the methods of multicolor SRS imaging and describes an overview of biomedical applications.