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Optical mapping of anatomical reentry in monolayers of cultured neonatal rat cardiac myocytes

There were two primary objectives in the study. First, we present a novel macroscopic optical method with high sensitivity to record transmembrane potentials, V/sub m/, in monolayers of cultured cardiac myocytes. Second, using this technique we demonstrate, for the first time, electrically-induced, sustained reentrant activity in a two-dimensional (2-D) cell culture preparation.