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Investigation of the therapy potential of borax pentahydrate in glioblastoma multiforme cell line

Recent studies with natural and synthetic boron compounds suggest that these compounds may be effective in the prevention and treatment of cancer. In this context, a synthetic boron compound, Borax pentahydrate (BPH), is aimed to be tested for cytotoxic effect on the glioblastoma multiforme (GBM) model U-87MG cell line representing fourth stage brain cancer in terms of induction of apoptosis and autophagy. Changes in apoptosis and autophagy rates of the research group treated with the specified IC 50 dose of BPH were determined by fluorescence-based microcapillary cytometry kit (Muse® Annexin V & Cell Death and Muse® Autophagy LC3-antibody based kit). The data obtained were evaluated using GraphPad Prism 5 statistical program. The IC 50 value of BPH in the U-87MG cell line was determined to be 2454μM for cytotoxicity. On the other hand, apoptosis rate was determined as increased 12.79 folds in the BPH treated group compared to the control group, while elevation of autophagy rate is determined as 1.2 folds. In the light of these data, while the cytotoxic effect of BPH on U-87MG cells, which is a model for GBM, is designated, it is understood that cell death pattern occurs through apoptosis instead of autophagy. In addition to these, it is thought that with the support of other new studies BPH may be an alternative agent that can be used in the treatment of GBM.