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Fluid shear stress alters ahnak expression and association with the L‐type voltage sensitive calcium channel in osteoblasts

Mechanical strains encountered through everyday activities are necessary for skeletal development and function, yet the cellular mechanisms necessary for this response are unknown. The earliest reaction to mechanical strain caused by fluid shear in osteoblasts is a rapid increase in intracellular calcium that occurs through activation of the L‐type voltage sensitive calcium channel (LVSCC). This calcium influx is necessary to induce bone formation, in vivo. The actin cytoskeleton of osteoblasts also responds to mechanical strain with an increase in formation of stress fibers. Ahnak, a 700kD protein has been found to interact with both the LVSCC and the f‐actin of the cytoskeleton. We propose that ahnak bridges the cytoskeleton to the LVSCC and that fluid shear will alter the expression of this protein in a time dependent manner. We found that ahnak protein levels rapidly decreased in osteoblasts within 1 hour of the onset of fluid shear. Ahnak levels returned within 6 hrs of shear onset, suggesting that ahnak is rapidly degraded and synthesized or is stored in discrete locations within the cell. Cell fragmentation studies to identify ahnak storage sites within the cell will be correlated with cytoskeleton disruption and immunofluorescent imaging to determine changes in ahnak localization during application of shear.