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Sumoylation System Replication Protein and the Plant Interaction between a Geminivirus

(TYLCSV) encode a protein, RepAC1 (or Rep), that is essential for viral replication. Rep/RepAC1 is anoligomeric protein that binds to double-stranded DNA, catalyzes cleavage and ligation of single-stranded DNA,and is sufﬁcient for host induction. It also interacts with several host proteins, including the cell cycleregulator, retinoblastoma, and essential components of the cell DNA replication machinery, like proliferatingnuclear cell antigen (PCNA) and RFC-1. To identify other cellular proteins that interact with Rep/RepAC1protein, a