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Studies on the vitamin B12 binding capacity in the hypophysectomized rat stomach.

The influence of the endocrine glands on hemopoiesis is being actively investigated by many authors (1-3). No information, however, on the relation ship between pituitary function and B,2 binding capacity in the stomach was available hitherto. In the present study, the authors attempted to investigate the way in which pituitary func tion affect the B12 binding capacity in the stomach using hypophysectomized rats. Wistar strain rats weighing about 150 g were used. Hypophysectomy was carried out by auricular approach under light anesthesia with sodium pentobarbital. Experiments were carried out on the 3, 5, 7, 14 th days after operation. Rats were exsanguinated. Their stomachs were removed immediately, trimmed of fat, washed throughly with running tap water and stored in a deep freezer. The extract was prepared as follows: the stomach was minced with scissors and homogenized with 10ml of physiological saline solution at 4•‹ using a blar ing Blendor. The homogenate was centrifuged at 3,000 rpm for 20 minutes. The pH of the opalescent supernatant fluid was found to be between 6-7. Each supernatant was measured for its N content, and determination of B12 binding power of the stomach was carried out in the following ways.