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CORRELATIVE QUANTITATIVE NANOMECHANICAL MAPPING AND CONFOCAL IMAGING OF LIVING CELLS BY SCANNING ION-CONDUCTANCE MICROSCOPY

Quantitative nanomechanical mapping (QNM) of single cell via Scanning Ion-conductance Microscopy (SICM) is a novel method of studying cell mechanical properties. SICM allows to topography mapping with lateral and vertical nanoscale resolution, based on working principles. Moreover, it is possible to perform stiffness mapping simultaneously, due to applying force to living cell surface, whose nature is intrinsic colloidal pressure between nanopipette tip and cell membrane (Clarke et al., 2013, 2016). Nanoscale diameter of nanopipette tip allows to obtain cell QNM with nanoscale resolution and to avoid cell damage due to of small values of applying force and non-contact scanning. As it was reported previously (Kolmogorov et al., 2021), SICM successfully used for detection of Young’s