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Mitochondrial-targeted deep-red fluorescent probe for ATP and its application in living cells and zebrafish.

A novel mitochondrial-targeted deep-red fluorescence ATP probe, NIR-A, is reported. The probe showed a fast, selective, and reversible response for ATP with a significant turn-on fluorescence signal at 663 nm with a large Stokes shift of 81 nm. Additionally, the introduction of TPP enabled TPP-endowed NIR-A to be enriched predominantly in the mitochondria. NIR-A was successfully applied to monitor ATP fluctuation in Ramos cells and zebrafish in real-time with good biocompatibility.