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Biochemical studies of bacterial sporulation and germination. XX. Phosphate metabolism during germination.

Abstract During germination in simple or complex media, the 3-phosphoglyceric acid content of dormant spores was rapidly utilized, and inorganic phosphate was generated from endogenous sources. The uptake of Pi from the medium during the first 40 min of germination in a complex medium or in glucose was extensive as compared to germination initiated by l-alanine or KBr. In the complex medium Pi was incorporated into acid-insoluble material (RNA) within 5 to 10 min. The principal acid-soluble products were glucose-6-P, fructose diphosphate, ATP, and l-α-glycerophosphate. The hexose phosphates increased throughout the first 40 min of germination. The level of α-glycerophosphate passed through a maximum in a complex germination medium, but not in the other media. These results indicate that energy-yielding metabolism begins very early in germination. Endogenous reserves, such as phosphoglyceric acid, may represent the earliest energy source, and at least part of the Embden-Meyerhof pathway becomes functional within a few minutes after germination is initiated. These results do not necessarily imply the absence of a pathway of direct oxidation of hexose monophosphates, but intermediates specific to this pathway were not found.