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Investigation of Barrett's esophagus by ultraviolet and visible fluorescence emission and excitation spectroscopy

In this work, ultraviolet and visible emission and excitation fluorescence spectroscopy was performed on excised human gastrointestinal tissue for the purpose of determining whether fluorescence measurements can be used to detect and monitor patients with BarrettOs esophagus. Tissue samples were acquired from eleven esophagectomy patients. For each excised esophagus, spectral measurements were taken from multiple locations of normal, malignant and, when present, suspected BarrettOs tissue. At each site scanned, a biopsy sample was removed for histopathology and the results were correlated with spectral measurements. Differences were observed in the spectra from BarrettOs, normal, and malignant esophageal tissue. Initial results indicate that these differences in spectra can clearly distinguish normal, BarrettOs and malignant tissue, and may allow in vivo optical detection of dysplasia in patients with BarrettOs esophagus during endoscopic examinations.