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Action of chloramphenicol and cycloheximide on Saccharomyces carlsbergensis: batch and continuous studies.

The kinetics of cycloheximide and chloramphenicol inhibition of S. carlsbergensis were studied in batch and continuous culture. Cycloheximide caused a reduction in the growth rate and the relationship that growth rate was proportional to minus twice the logarithm of the cycloheximide concentration was proposed for the concentration range O· 003-0' 05 mg/I. In batch culture the A group cytochromes were little affected by cycloheximide while the C group (C+C1) showed the most marked changes. Continuous culture revealed little further information due to the indirect effect of the decreasing growth rate on Qo., Qeo., QEtOH and the A, B, and C group cytochromes.