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[The expression of Sonic Hedgehog during late bell stage by Western Blot and in situ hybridization].

OBJECTIVE
To investigate the expression and biological effect of Shh during late bell stage by morphological and semi-quantitative analysis.


METHODS
Tooth germs were selected from new born Bal b/c mouse (P1, P2, P3, P5, P7). Semi-quality of Shh was measured by Western Blot and the expression place and strength of Shh were observed by in situ hybridization.


RESULTS
Shh was expressed in the ameloblast layer during late bell stage; the expression strength was high in secretive period and decreased with development; the active N-section was detectable before P3.


CONCLUSION
Shh expresses specially in the ameloblast layer in late bell stage, and expression quality is related to the function of ameloblasts.