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OPTIMIZATION AND PRODUCTION OF β-GLUCOSIDA- SE FROM THE PLANT PATHOGENIC FUNGI Bipolaris oryzae UNDER SOLID-STATE CULTIVATION OF VETIVER GRASS USING RESPONSE SURFACE METHODOLOGY

Solid-state cultivation of vetiver grass using Bipolaris oryzae was performed. L-asparagine concentration, initial pH and raw material-to-moisture were optimized in culture flasks using response surface methodology with respect to specific activity of β-glucosidase in the culture. Lasparagine concentration at 2.70 g l -1 , pH of 6.3, and raw material-to-moisture 1: 3.35 (w/v) were found to be the most optimum. Optimized parameters were used for laboratory scale-up in static tray fermenter. The maximum specific activity of β-glucosidase at 1259.72 U mg-protein -1 was obtained after 7 day incubation in static tray fermenter.