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Preparation of Bioactive Hydroxyapatite on Pure Titanium

In this study, a hydroxyapatite (HA) was coated on a pure titanium surface by means of a complex oxidation and hydrothermal treatment. First an anodic oxidation was done on the titanium plates, followed by micro-arc oxidation. The HA-coated specimens and pure titanium specimens were immersed in SLB for 1, 5, and 10 days, respectively, to study their electrochemical behavior. The corrosion currents of HA-coated specimens were less than pure titanium specimens. This indicated that HA coating prevented surface metal ions of the implant from dissolving, thereby, reducing the tissue toxicity. The cytotoxic effect on fibroblasts L929 cells was measured by cell counting after being seeded for 2, 4, 8, 12, and 24 h. The number of surface cell attachments on the HA-coated specimens was much greater than on pure titanium specimens. The morphology of the cells on the HA coating had normal shapes and spread well with some cells climbing onto surface pores while cells on the pure titanium were oval shaped. The results confirm that the cell compatibility on HA-coated ion titanium surfaces is much better than pure titanium.