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Working Conditions in the Laboratory for Raman Microscopy

Raman spectroscopy/microscopy technique allows monitoring of various chemical processes in real time, and material characterization in terms of the distribution of individual chemical components via method known as Raman mapping. Equipping laboratory for this technique requires following: a stable supply of electricity, anti-vibration table for the instrument, constant temperature and controlled source of light. Prior to beginning the measurement it is necessary to select the laser of the appropriate wavelength and to calibrate the instrument. The instruments that are used in Raman spectroscopy/microscopy allow monitoring of various chemical processes in real time, and material characterization in terms of the distribution of individual chemical components via method known as Raman mapping. To carry out complex analyzes of sample’s surface it is necessary to possess a Raman microscope with motorized stand that allows automatic scrolling and analyzing previously given points on the sample. The resultant spectra form a “chemical map” of the sample. How to cite this book chapter: Lević, S. 2019. Working Conditions in the Laboratory for Raman Microscopy. In: Vucelić Radović, B., Lazić, D. and Nikšić, M. (eds.) Application of Molecular Methods and Raman Microscopy/ Spectroscopy in Agricultural Sciences and Food Technology, Pp. 151–159. London: Ubiquity Press. DOI: https://doi.org/10.5334/ bbj.j. License: CC-BY 4.0 152 Application of Molecular Methods and Raman Microscopy In order to work in accordance with the requirements of the sample analysis, laboratory for Raman microscopy should meet several basic conditions. First of all, microscope needs a stable supply of electricity. It is necessary to eliminate sources of vibration that could adversely plug on the operation of the microscope, by placing it on the appropriate anti-vibration table (Figure 1). In addition, air-conditioning in the room is necessary in order to ensure conditions for the work of staff but also the stable temperature required for the smooth operation of the device. In addition to the elimination of the effect of vibration, it is necessary to eliminate the influence of ambient light on the analysis in progress (Figure 2), which is achieved by shutting down the lights during excitation of the sample or by using microscope equipped with special curtain. Small table lamps that don’t affect the operation of the device can be used. 1 Selection of laser of the appropriate wavelength i.e. choice of