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EUCAST Definitive Document E.DEF 3.1, June 2000: Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by agar dilution.

Dilutionmethods are used to determine theminimum inhibitory concentrations (MICs) of antimicrobial agents and are the reference methods for antimicrobial susceptibility testing against which other methods, such as disk di¡usion, are calibrated. MIC methods are widely used in the comparative testing of new agents. In clinical laboratories they are used to establish the susceptibility of organisms that give equivocal results in disk tests, for tests on organisms where disk tests may be unreliable, and when a more accurate result is required for clinical management. In dilution tests, microorganisms are tested for their ability to produce visible growth on a series of agar plates (agar dilution) or in microplate wells of broth (broth microdilution) containing dilutions of the antimicrobial agent. The lowest concentration of an antimicrobial agent that will inhibit the visible growth of amicroorganism is known as theMIC. The agar dilution method described in this document is based on that described in the report of an international collaborative study of antimicrobial susceptibility testing [1] and is very similar to those described and recommended in many countries, including France [2], Germany [3], Norway [4], Sweden [5], theUK [6] and theUSA [7].