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937 HUMAN NEONATAL POLYMORPHONUCLEAR LEUKOCYTE (PMNL) ANTIBODY-DEPENDENT CELLULAR-CYTOTOXICITY (ADCC) TO HERPES SIMPLEX VIRUS (HSV) INFECTED CELLS

ADCC is an important antiviral immune mechanism. Human neonatal cells have lymphocyte ADCC and functional PMNL defects. We therefore analyzed the ability of dextran sedimented, ficoll hypaque centrifuged purified cord blood PMNL from full term healthy infants to kill HSV infected target cells in an 18 hr 51 Cr release assay. At an effector to target cell ratio (E:T) of 100:1, ADCC activity of PMNL from 10 cord blood samples (45.1±6.2) was no different than that of PMNL from healthy adults (46.8±9.6). E:T analysis from 100:1 to 30:1 revealed identical values for all 8 sets of neonatal and adult PMNL tested. Antibody concentration requirements were similar for all 3 sets of neonatal and adult cells tested with activity at immune sera concentrations of 1/20 but none at 1/1000. There was no difference in ADCC activity of PMNL from neonates born by vaginal (44.1 ± 11.3) or Caesarian section (45.8 ±7.8) deliveries.These data demonstrate the normal function of cord blood PMNL in ADCC and highlight the importance of antibody against HSV in neonatal HSV infection.Supported by NIH grants 1 PO1 HD 13021, and AI 14450