{'fraction_non_alphanumeric': 0.019524617996604415, 'fraction_numerical': 0.012733446519524618, 'mean_word_length': 5.172774869109948, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
FREE FATTY ACID ENHANCES EXPRESSION OF INFLAMMATORY MARKERS IL6, TLR2, TLR4 IN C2C12 MUSCLE CELLS

It is well known that obesity is increasing as worldwide epidemic and it is often accompanied by increased metabolic disorders. Having an insight awareness about obesity-related conditions will be a strategy to protect against obesity-related dysfunctions. In the present study, we differentiate C2C12 myocytes into mature myotubes. Then the cells were treated with free fatty acid (FFA) to mimic the obese microenvironment.The result showed that the level of IL6 mRNA, a key inflammatory cytokine, was significantly higher in the myotubes treated with FFA compared to the myotubes treated with the control medium. Consistent with this, the level of TLR2 mRNA, an important regulator of inflammatory responses, was markedly increased in the cells treated with FFA compared to the control cells. Additionally, mRNA level of TLR4, another key regulator of the inflammatory response, was highly expressed in the FFA treated cells compared with the control cells. These data demonstrate that the C2C12 myotubes cultured with FFA can be an in vitro obese model for further researches.