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Anticoagulant activity of a synthetic heparinoid in relation to molecular weight and N-sulfate content.

Addition of chlorosulfonyl isocyanate to C==C bonds in cis-1,4-polyisoprene and reaction of the adduct with NaOH resulted in the formation of a water-soluble polyelectrolyte with N-sulfate and carboxylate groups. The polyelectrolyte showed anticoagulant activity and it was found, just as with heparin, that the activity was related to molecular weight and N-sulfate content.