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Mutation analysis of the role for the carboxy-terminus encoding region in NGF-induced stabilization of GAP-43 mRNA.

NGF-induced stabilization of rat GAP-43 mRNA has been shown to be mediated by the 292 nt segment including both 3' untranslated region and a 115 nt segment encoding the carboxy-terminus region. Further analysis shows that the 115 nt segment, which is additionally inserted just downstream from the open reading frame, abolished most of the stabilizing effect of NGF. On the contrary, an in-frame insertion of the 115 nt segment just upstream from the termination codon does not affect the stabilization. Frameshift mutation of the segment encoding the carboxy-terminus region also abolishes the NGF effect. These findings suggest an essential role for the direct translation of the mRNA segment encoding the carboxy-terminus region in the stability regulation mediated by the 292 nt segment. In addition, presented results suggest a role for the cis interaction between the sub-segments in the 292 nt segment, which are the part of the 3'untranslated region and the segment for the carboxy-terminus.