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Fast releasing allergens from some organisms living in house dust

The influence of extraction procedures and culturing methods of material used for the preparation of allergenic extracts on correlation patterns found in allergological testing (skin test and RAST) was investigated. In our laboratory a short extraction procedure performed at 0 o C was used for Aspergillus repens, A pemalloides, Wallemia sebi, their rearing media and non-inoculated medium. For the commercially available extracts from house dust, house-dust mile, pollen of Dactvlus glomeraia and A. penicilloides a longer procedure (several days) performed at room temperature was used. Statistical analysis showed a separation of all test results into two clusters, each cluster being composed of correlations between extracts from only one of the manufacturers. Even the extracts of the same fungal species prepared by the different manufacturers did not show any correlation The correlations found between the short time incubated extracts of the xerophilic lungi and their rearing media could be explained by genetical and biochemical relationships between these fungi depending on ecological conditions. However, while the correlation found between house dust and house-dust mite is understandable, correlations lound between long time incubated extracts from house-dust mite and D. glomeraia or A penuilloules may be nonsense correlations, that do not adequately describe the i/i v/vo situation. The similarity of these extracts is presumably artificially created during extraction.