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Effects of Darkness On Fruit Ripening In Grape And Tomato By RNA-Seq


 Light is an important external environment factor which influences all aspects of plant growth and development, including fruit ripening. Transcription regulation of light will provide insights into effect of light for fruit ripening. In this study, we treat grape and tomato fruit with dark or light, and by the RNA-seq method, compare transcriptional level between dark-treated and light-treated fruits. Additionally, we analyzed the metabolome of freshly cut fruit samples in two-treated conditions. In this study, transcriptomic and metabonomic analyses were used to evaluate the effects of shading on the ripening of different climacteric types of fruits (tomato and grape). we showed that shading treatment could inhibited fruit development by down-regulating factors related to fruit ripening-related growth and development. The differentially expressed genes (DEGs) from treated or non-treated samples with dark were mainly enriched in sugar metabolism and signal transduction, plant hormone biosynthesis and secondary metabolism. At the same time, we used metabonomics to detect different secondary metabolites in tomato and grape. Based on the above data, we established a possible model of shading treatment regulating fruit quality of tomato and grape. We found that the expression of DEGs in sugar metabolism and signal transduction, plant hormone biosynthesis and secondary metabolism differ between tomato and grape after shading treatment, which helps to clarify the possible mechanism of how the response to light in climacteric and non-climacteric fruit.