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Role of DE-Cadherin in Neuroblast Proliferation, Neural Morphogenesis, and Axon Tract Formation in DrosophilaLarval Brain Development

In the wild-type brain, the Drosophila classic cadherin DE-cadherin is expressed globally by postembryonic neuroblasts and their lineages (“secondary lineages”), as well as glial cells. To address the role of DE-cadherin in the larval brain, we took advantage of the dominant-negative DE-cadexconstruct, the expression of which was directed to neurons, glial cells, or both. Global expression of DE-cadex driven by a heat pulse during the early second instar resulted in a severe phenotype that included deficits in neural proliferation. Neuroblasts appeared in approximately normal numbers but had highly reduced mitotic activity. When the DE-cadex construct was driven by the glial-specific driver gcm-Gal4, the effect of DE-cadex on neuroblast proliferation could be replicated, which indicates that DE-cadherin acts in glial cells to promote proliferation of neuroblasts. Expression of DE-cadex in neurons, cortex glia, or both results in abnormalities in cortex layering and in trajectories of secondary axons. In the wild-type brain, neuroblasts and neurons generated at different time points are arranged concentrically around the neuropile, with the DE-cadherin-positive neuroblasts and young secondary neurons at the surface, followed by older secondary neurons and primary neurons. Axons of secondary lineages follow a straight radial course toward the neuropile. Processes of glial cells located in the cortex form a scaffold, called trophospongium, that enwraps neuroblasts and neurons. Expression of DE-cadex in neurons, cortex glia, or both disrupted the regular placement of neuroblasts and secondary neurons and resulted in abnormal trajectories of cell body fiber tracts. We conclude that DE-cadherin plays a pivotal role in larval brain proliferation, brain cortex morphogenesis, and axon growth.