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The dietary antioxidant resveratrol is a functional scavenger of peroxynitrite in vitro

Resveratrol (Rsv) is a polyphenol found naturally in dietary products such as red wine and peanuts and suggested to have antioxidant properties. However, the mechanism(s) by which it may function as an antioxidant is unclear. Oxidants are a major contributor to cellular damage in numerous pathological states. We have previously shown that Rsv reduces the formation of NO‐derived reactive nitrogen species (RNS) in the kidney during sepsis. Thus, we propose that Rsv is able to protect against RNS‐mediated cellular damage by acting as an RNS scavenger. To test this, Rsv was reacted in vitro with varying concentrations of the RNS, peroxynitrite (ONOO−). LC/MS/MS revealed the formation of Rsv dimers, trimers, and nitrated Rsv products. Next, nitration of bovine serum albumin by ONOO− was used as a functional assay. Rsv blocked the formation of nitrotyrosine adducts on bovine serum albumin in a concentration‐dependent manner, indicating Rsv can act as a functional scavenger of ONOO−. Furthermore, Rsv was more potent at inhibiting nitration than the known RNS scavenger, N‐acetylcysteine. These data indicate that Rsv can act as a potent RNS scavenger and that this activity may explain its protective effects in the kidney during sepsis. Moreover, uncovering the RNS scavenging ability of Rsv contributes to the understanding of its other reported beneficial effects such as increasing longevity. Supported by NIH DK075991.