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effective role of vitamin D and omega-3 on rats with induced Crohn’s disease

Crohn's disease is an irreversible inflammatory disease, mainly affecting the gastrointestinal tract. The aim of present study was to investigate the effect of dietary vitamin D and omega 3 supplementation on rats with induced Crohn’s disease. Twenty – four adult male albino rats weighing (160 gm) were divided into 4 groups. The 1st group (n=6) was fed on the basal diet. The 2nd to 4th of rats (n=18) were injected with (10 mg/kg for 3 days) of indomethacin at the end of the experiment to induce Crohn’s disease. Groups (3 and 4) were fed on basal diet and given orally 1000/IU/kg/ b.w. of vitamin D and 600 mg/kg/b.w. of omega 3 respectively. At the end of the experimental period (8 weeks), rats were scarified. Biochemical analysis of the tested parameters was determined. The Results indicated that indomethacin Crohn’s rats showed a significant (P< 0.05) decrease in serum albumin, serum Catalase and serum Glutathione peroxidase compared to the negative control one, while serum liver function, MDA, CRP, and immunity proteins were significantly increased. 