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Characterization of the ICR489 Gene of Infectious Spleen and Kidney Necrosis Virus of Siniperca chuatsi

ICR489 gene of infectious spleen and kidney necrosis virus(ISKNV)of Siniperca chuatsi were sequenced.The open reading frame of the gene is 1 011 nucleotides long,encoding a putative protein of 337 amino acids with M w of 38 270. The 5' noncoding region has TATA box.A stem loop and palindrome are contained upstream of the translation start codon.The results of comparison of the homology with other iridoviruses ICR489 genes showed that ISKNV ICR489 gene has lower identities with others.Alignment of amino acid sequences and a phylogenetic tree of ICR489 genes of four iridoviruses were constructed.The results indicated that ISKNV is different from the viruses of the genus Ranavirus and Lymphocystivirus.