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Localization of functional centers on the prokaryotic ribosome: immuno‐electron microscopy approach

Immuno‐electron microscopy studies on the morphology of the Escherichia coli ribosome and the topography of its functional centers are summarized. A three‐dimensional model of the ribosome with indications of the mRNA‐binding site, the peptidyl‐transferase center, the EF‐Tu and EF‐G‐binding sites and the tRNA‐binding sites in given. Thus, the mutual arrangement of the centers corresponding to the main functional activities of the ribosome during the process of translation is presented.