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The Gut Microbiota Determines the High-Altitude Adaptability of Tibetan Wild Asses (Equus kiang) in Qinghai-Tibet Plateau

It was acknowledged long ago that microorganisms have played critical roles in animal evolution. Tibetan wild asses (TWA, Equus kiang) are the only wild perissodactyls on the Qinghai-Tibet Plateau (QTP) and the first national protected animals; however, knowledge about the relationships between their gut microbiota and the host's adaptability remains poorly understood. Herein, 16S rRNA and meta-genomic sequencing approaches were employed to investigate the gut microbiota–host associations in TWA and were compared against those of the co-resident livestock of yak (Bos grunnies) and Tibetan sheep (Ovis aries). Results revealed that the gut microbiota of yak and Tibetan sheep underwent convergent evolution. By contrast, the intestinal microflora of TWA diverged in a direction enabling the host to subsist on sparse and low-quality forage. Meanwhile, high microbial diversity (Shannon and Chao1 indices), cellulolytic activity, and abundant indicator species such as Spirochaetes, Bacteroidetes, Prevotella_1, and Treponema_2 supported forage digestion and short-chain fatty acid production in the gut of TWA. Meanwhile, the enterotype identification analysis showed that TWA shifted their enterotype in response to low-quality forage for a better utilization of forage nitrogen and short-chain fatty acid production. Metagenomic analysis revealed that plant biomass degrading microbial consortia, genes, and enzymes like the cellulolytic strains (Prevotella ruminicola, Ruminococcus flavefaciens, Ruminococcus albus, Butyrivibrio fibrisolvens, and Ruminobacter amylophilus), as well as carbohydrate metabolism genes (GH43, GH3, GH31, GH5, and GH10) and enzymes (β-glucosidase, xylanase, and β-xylosidase, etc.) had a significantly higher enrichment in TWA. Our results indicate that gut microbiota can improve the adaptability of TWA through plant biomass degradation and energy maintenance by the functions of gut microbiota in the face of nutritional deficiencies and also provide a strong rationale for understanding the roles of gut microbiota in the adaptation of QTP wildlife when facing harsh feeding environments.


INTRODUCTION
Adaptation is a core process in biological evolution and has attracted research attention since the time of Charles Darwin (Boyd, 2010). The microbial consortia in herbivore digestive tracts have co-evolved with their hosts. They convert lignocellulose hydrolysates into short-chain fatty acids (SCFAs) and play vital roles in host digestion (Kumar et al., 2021), health (Shreiner et al., 2015), immunity , and adaptability (Ley et al., 2008). Advances in sequencing technology over the past decade have provided novel insights into microbiome co-evolution and adaptability. Furthermore, herbivore adaptation mediated via the gut microbiota has attracted a great deal of research attention (Liu et al., 2020d;Song et al., 2020;Fu et al., 2021).
The Qinghai-Tibet Plateau (QTP) is the highest plateau in the world, known as "the third pole" (Qu et al., 2020). It has special environmental conditions, such as high altitude, accompanied by low oxygen, severe cold, and high ultraviolet radiation. Yak (Y; Bos grunniens) and Tibetan sheep (S; Ovis aries) are the main indigenous livestock on the QTP. They have been grazed and used by local herdsmen for meat, milk, and fuel (the dry feces) for several centuries (Cui et al., 2020). Modification of the intestinal microbiomes of these high-altitude ruminants in response to the unique ecological environment of the QTP has been extensively studied (Ma et al., 2019b;Liu et al., 2020aLiu et al., ,c, 2021Fan et al., 2021). By way of illustration, S adapt to changes in the plant phenological period by altering their rumen microbial community structure and function (Liu et al., 2020a). Zhang et al. (2016) evaluated the production of SCFAs and emission of methane by in vitro rumen fermentation technique and found that Y accumulates high SCFAs concentrations in their rumen and attenuates methane emissions via microbial intervention, thereby harvesting energy more effectively than cattle. Tibetan wild ass (Equus kiang) is the only wild perissodactyl in the QTP. It has been endemic to the cold, hypoxic (4,000-7,000 m a.s.l.) montane, and alpine grassland for thousands of years, and often faces the problem of imbalance between herbage supply and the host's requirement (Schaller, 2000). Gut microbes are considered to be the link that converts herbage nutrients into the host's own nutrients; however, knowledge about whether these gastrointestinal microbes have special mechanisms to adapt to the ecological environments is limited. Only Gao et al. (2019) reported that Tibetan wild asses (TWA) adapt to the extreme environments of the QTP when their gut microbiota alter their fatty acid metabolism pathways, and Liu et al. (2020b) stated that TWA was superior to the domestic donkey in terms of gut microbiota community, function, and disease resistance under similar forage intake. Although the above work has described the structure and function of gut microbiota of TWA, the interaction between gut microbiota and the host and the response mechanism under environmental stress need to be further studied.
The Three-River-Source National Park is located in the hinterlands of the QTP and has a total area of 123,100 km 2 . It is the source place of the Yangtze, Yellow, and Lancang rivers. It is dominated by mountainous landforms with complex topographies and harbors vital habitats and biological germplasms of rare wild animal species (Qiao et al., 2018). As the awareness of ecological protection has increased in recent years, the number of wild animals such as TWA has increased to 36,000 in Three-River-Source National Park (Zhao et al., 2020). However, competition for forage often occurs between wild and domestic animals in this area. The overlapping habitats of wild and domestic animals in the region have enabled us to study the unique adaptive mechanisms of their intestinal microorganisms.
The large intestine, which included the cecum and the colon, harbored a higher bacterial diversity (Li et al., 2017) and is an important site of plant-fiber fermentation for asses. Analyses of fecal bacteria can characterize gut microbiota because the microorganisms in the feces resemble those in the large intestine (Zhao et al., 2015). In the present study, we used fresh feces from TWA, Y, and S as research objects and multi-omics methods to distinguish the unique gut microbial composition and highaltitude adaptation mechanisms of these animal groups. We speculate that there is a special microbial interaction mechanism in the gut of the TWA that is different from that of domestic animals. The aim of this study is to clarify the plateau adaptation mechanism of the TWA through their gut microbiota and provide a theoretical basis for investigations into the dietary adaptation of wild animals in QTP.

Sample Collection
Fecal samples were collected on 13 August 2017 from co-resident TWA, S, and Y near the Three-River-Source Alpine Meadow Integrated System Observation in Yangtze River Source Park (Qumarleb County, Yushu Prefecture, Qinghai Province, China; Figure 1). The Yangtze River Source Park has an average altitude of 4,300 m, and the vegetation types are mainly alpine meadows, supplemented by desertified grasslands. Y and S in the area were under the supervision of herdsmen and usually foraged freely on grassland with abundant, high-quality herbage. As the human population is sparse and water sources were abundant in the area, wild animals such as TWA also foraged here. This condition created a sympatric living environment for all animals in the area. However, herdsmen's interference usually forced TWA to forage on nearby slopes with relatively little or low-quality vegetation.
As all animals dwelt in their natural habitat, they were highly alert. Therefore, the group was followed from a distance during sampling. After the animals defecated and left the area, feces were carefully but promptly collected from different individuals. To avoid cross-contamination, each sample was gathered with a disposable polyethylene glove that was used only once and then discarded. Twenty-seven fresh fecal samples were collected from seven S, nine Y, and 11 TWA. After collection, ∼2 g of fresh fecal samples were placed in uniquely labeled cryotubes, frozen in liquid nitrogen, and stored at −80 • C until subsequent high-throughput sequencing. Other samples (∼50 g) were collected in sterile uniquely labeled zip lock bags, temporarily stored at 4 • C in an automobile refrigerator, transferred to a laboratory refrigerator (4 • C), and stored until subsequent apparent nutrient digestibility analyses. Fecal samples were collected within 1 d in their natural state. None of the animals received concentrate supplementation.
The herbage collection methods used here resembled those of Ma et al. (2019a). Briefly, ten 50 × 50 cm quadrats were randomly set at > 10-m intervals in an alpine meadow and the aboveground biomass was collected from them. To ensure that the forages collected were similar to those consumed by the animals, each quadrat was set on a forage with visible bite marks. Edible forage was collected, dried in a forced air stove at 60 • C for 72 h, milled, passed through a 1-mm sieve, and stored in watertight plastic bags until subsequent dry matter (DM) digestibility and nutrition analyses.

Chemical Analysis
The DM (oven method 930.15), crude protein (CP, Kjeldahl method 988.05), and ether extract (EE, diethyl ether extraction method 2003.5) contents were determined for forage-feces composites according to AOAC methods (AOAC, 2000). Acid detergent fiber (ADF) and neutral detergent fiber (NDF) were analyzed according to the methods of Van Soest et al. (1991).
Nonfibrous carbohydrate (NSC) was calculated as follows: The nutrition of forage are shown in Supplementary Table 1.

Determination of Host Apparent Nutrient Digestibility
Apparent nutrient digestibility was determined with acidinsoluble ash (AIA) as described by Liu et al. (2020b). Briefly, 5 g crushed forage and dried feces from the same individual were boiled in 50 mL of 4 N HCl for 30 min. The residue was filtered with a quantitative filter paper (Φ12.5 cm; NEWSTAR R , Hangzhou, China) and washed with hot distilled water until the filtrate was neutral. The residue and filter paper were ashed in a crucible in a muffle furnace (Beijing Zhongxing Weiye Instrument Co. Ltd., KSW-6-12) at 600 • C for 12 h. After complete combustion of the organic matter, the crucible and ash were weighed on an analytical balance and the AIA values of the forage and feces were obtained. The total apparent CP, NDF, and ADF in the gut were calculated from the dietary and fecal nutrient. AIA ratios are as follows (Kavanagh et al., 2001): where Z feces and Z diet are the nutrient concentrations (%) in the feces and forage, respectively, and AIA h and AIA f are the AIA concentrations (%) in the herbage and feces, respectively.

Short-Chain Fatty Acids (SCFAs) Analysis
Short-chain fatty acids were detected according to the method of Fan et al. (2020). Briefly, acetic acid and propionic acid were measured by propyl chloroformate (PCF) derivatization followed by gas chromatography-mass spectrometry (GC-MS) (Zheng et al., 2013). Approximately, 0.1 g of each ∼fecal sample was added to 1,000 µL of 0.005 M NaOH containing 5 µg/mL caproic acid-d3 internal standard (IS) and the suspension was homogenized. Then 500 µL of supernatant aliquot was transferred to a 15-mL capped centrifuge tube. Then 300 µL ultrapure water, 100 µL PCF, and 500 µL PrOH/Py solution (3:2, v/v) were added to the supernatant and the mixture was vortexed for 30 min. The derivatization included two extractions. In the first, 300 µL hexane was added to the mixture which was then vortexed for 1 min and centrifuged at 3,000 × g for 5 min. In the second, 200 µL hexane was added and 500 µL derivatized extract was collected in an autosampler vial and analyzed with an Agilent 7890A/5975C GC-MS (MSD; Agilent Technologies, Santa Clara, CA, USA).

Determination of Cellulolytic Activity
Cellulolytic activity was determined based on the reaction between 3,5-dinitrosalicylic acid (DNS) and reducing sugar generated from a cellulose degradation assay (Miller, 1959). Gut contents (0.1 g) were placed in microcentrifuge tubes and homogenized in 1 mL extraction buffer at 4 • C. The samples were homogenized on an ice bath and centrifuged at 8,000 × g and 4 • C for 10 min. The supernatants were used in the enzymatic assays. Endo-β-1,4-glucanase activity was determined with a cellulase (CL) activity assay kit (Beijing Boxbio Science & Technology Co. Ltd., Beijing, China) according to the manufacturer's instructions. One unit of cellulolytic activity was defined as 1 g tissue producing 1 µg reducing sugar (glucose equivalent) per min.

DNA Extraction and 16S rRNA Gene Illumina Sequencing
A fecal E.Z.N.A. R DNA kit (Omega Bio-tek, Norcross, GA, USA) was used to extract the total microbial DNA from ∼0.2 g fecal samples according to the manufacturer's protocols. The universal primers 341F (5 ′ -barcode-CCTACGGGNGGCWGCAG-3 ′ ) and 806R (5 ′ -GGACTACHVGGGTWTCTAAT-3 ′ ) were used to amplify the bacterial V3-V4 hypervariable region (Liu et al., 2020b). The barcodes were eight-base sequences unique to each sample. A 30-µL PCR amplification mixture was prepared for each sample and consisted of 15 µL of 2× Phanta Master Mix, 1 µL of each primer (10 µM), and 20 ng template DNA. The cycling parameters for the PCR amplification included an initial denaturation at 95 • C for 5 min followed by 30 cycles at 95 • C for 30 s, annealing at 55 • C for 30 s, elongation at 72 • C for 45 s, and a final extension at 72 • C for 5 min. All PCR products were detected on 2% agarose gels and purified with an AxyPrep DNA gel extraction kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer's instructions. They were then quantified with QuantiFluor TM -ST (Promega, Madison, WI, USA). The sequence library was generated with a Qubit R 3.0 fluorometer (Invitrogen, Carlsbad, CA, USA) and an Agilent Bio Analyzer system. The amplicons were pooled into equimolar sample concentrations. After quality assessment, the library was sequenced on an Illumina HiSeq 2500 platform (Nanjing Gene Pioneer Co. Ltd., Nanjing, China) and 250-bp paired-end reads were generated.

Enterotype Clustering
Enterotype clustering is widely used in humans (Arumugam et al., 2011;Costea et al., 2018). Genus-level relative abundance profiles of the samples were clustered by Jensen-Shannon divergence (JSD), B-C dissimilarity, and partitioning method (PAM) clustering in R v. 4.1.2 (R Core Team, Vienna, Austria). The Calinski-Harabasz (CH) index and the silhouette score were used to evaluate cluster robustness (Hildebrand et al., 2013). The PAM, CH index, and silhouette score were then applied to cluster enterotypes by the B-C dissimilarity and JSD methods, and the same results were obtained by both the methods (Supplementary Figure 1). Earlier studies suggested that B-C dissimilarity is strongly correlated with JSD (Arumugam et al., 2011;Guo et al., 2021) and revealed variations in the abundance of taxa with enterotypes. Hence, B-C dissimilarity was conducted at the genus level. The similarity percentage (SIMPER) method (https://rdrr.io/rforge/ vegan/man/simper.html) was used to identify and rank the contribution of each genus among the enterotype groups (Clarke, 1993).

Shotgun Meta-Genomic Sequencing, Assembly, and Annotation
Sixteen samples were selected (TWA, n = 7; Y, n = 4; S, n = 5) for meta-genomic sequencing. Genomic DNA quality and purity were assessed with a NanoPhotometer R spectrophotometer, a Qubit R dsDNA assay kit, and a Qubit R 2.0 fluorometer (Life Technologies, Waltham, MA, USA). Genomic DNA with OD in the range of 1.8-2.0 and mass > 1 µg was used as the input for sequencing library generation with a NEBNext R Ultra TM DNA library prep kit (New England Biolabs, Ipswich, MA, USA). Sequences with index codes were added to split each sample. The samples were sonicated to generate ∼350-bp DNA fragments, which were then end-polished, A-tailed, and ligated with full-length adaptors for PCR amplification. The PCR products were cleaned and extracted in an AMPure XP system (Beckman Coulter, Brea, CA, USA). Libraries were prepared in a cBot cluster generation system (Illumina, San Diego, CA, USA) according to the manufacturer's protocols. After cluster generation, the prepared library was sequenced on an Illumina HiSeq 2500 platform (Novogene Biological Information Technology Co., Beijing, China), and 150-bp paired-end reads were generated.
To get high-quality clean reads, sequences with low base quality (quality value < 38%; > 40 bp) and N bases > 10 bp were removed to obtain high-quality clean reads. Bowtie2 (https://www.encodeproject.org/software/bowtie2/) was used to remove reads contaminated by adaptor and host sequences (Karlsson et al., 2012(Karlsson et al., , 2013Scher et al., 2013). The configuration parameters were: -end-to-end, -sensitive, -I 200, and -X 400. After filtering, clean data with k-mers = 55 were assembled into scaftigs with SOAPdenovo (https:// github.com/aquaskyline/SOAPdenovo2; Luo et al., 2012) using the following configuration parameters: -d 1, -M 3, -R, -u, and -F. N50 length was used to evaluate the quality of the assembly. MetaGeneMark v. 2.1.0 (https://github.com/gatechgenemark/MetaGeneMark-2; Nielsen et al., 2014;Oh et al., 2014) was used to predict the contigs (≥500 bp) in each sample. Open reading frames (ORFs) derived from the assembled contigs were clustered into a non-redundant dataset with CH-HIT (Li and Godzik, 2006). The configuration parameters were: -c 0.95, -G 0, -aS 0.9, -g 1, and -d 0. Bowtie2 was used to count the reads generated from the re-aligned reads and reduce the number of redundant genes. A gene catalog was obtained from the nonredundant genes with > 2 reads. After read filtering, DIAMOND (https://github.com/bbuchfink/ diamond; Buchfink et al., 2015) was used to align the unigenes with bacteria and archaea extracted from the NR database v. 2018.01 (National Center for Biotechnology Information (NCBI), Bethesda, MD, USA; blastp; e-value ≤ 1e-5) for taxonomic profile annotation. For gene function annotation, the unigenes were blasted with the CAZy, KEGG, and eggNOG functional databases in DIAMOND.

Statistical Analysis
Standard R commands were performed to identify the differences in relative abundance across groups. Kruskal-Wallis (multiple groups) and Dunn's post-hoc (two-sample comparisons) tests were used to evaluate significant differences in the nonparametric profiles. The Mann-Whitney U-test was used to calculate the contributions of the gut microbiota associated with the various enterotypes. R v. 4.0.2 generated Sankey plots or boxplots to visualize the relative abundances of the microbial taxa and concentrations of SCFAs across hosts. B-C dissimilarity and nonmetric multidimensional scaling (NMDS) were run in the vegan package v. 2.5-7 of R (Oksanen et al., 2013). To identify the microbial taxa that best represented variations in this parameter across hosts, an indicator analysis was conducted in the indicspecies package v. 1.7.9 in R. Multiptt function with 9,999 permutations was performed on the list of species correlated with a group of samples. The r.g. function was used to determine the correlations between the binary vectors (Guo et al., 2021). A Mantel test with 9,999 permutations calculated the correlations between gut environmental factors and microbial community data and was run in the linkETv. 0.0.3.3 package of R (https://github.com/Hy4m/linkET), and was based on calculated environmental (Euclidean) and taxonomic (Bray-Curtis) distances. Pairwise Pearson's correlation analyses were implemented using the "quickor" function in ggcor to evaluate the significance of the relationships among environmental parameters. Heat maps of the glucoside hydrolase family genes were generated with the pheatmap v. 1.0.12 package in R.

Forage Apparent Nutrient Digestibility and Cellulytic Activity
As shown in Table 1, the apparent nutrient digestibility of CP in the TWA group (81.26%) was significantly higher than that of S (P < 0.05), but not significantly with Y. The cellulytic activity in the TWA group (517.19 U/g) was significantly higher than that of Y and S (P < 0.05). No significant differences were detected for DM (P = 0.215), NDF (P = 0.171), and ADF (P = 0.313) digestibility among the three groups.   (Figure 2A,  Supplementary Table 3). The similarity of gut flora was | diversity metric is also tested to identify differences in variance among treatments. Significant differences are determined using 999 permutations of the betadisper function in the vegan v. 2.5-6 package of R. All boxplots distribution are tested by non-parametric Kruskal-Wallis and Dunn post hoc tests with FDR-corrected P-value, boxplots center values indicate the median, and whiskers represent 0.75 times the interquartile range. * < 0.05, ** < 0.01, no * mean no significant difference. also exhibited at the genus level in both TWA and domestic animals, with p-1088-a5 gut group (11.61% of total average), Akkermansia (7.85%), and Ruminococcaceae UCG-005 (7.51%) the most abundant across the hosts ( Figure 2C, Supplementary Table 4).
The dissimilarity was manifested in alpha and beta diversity. The Shannon diversity in TWA was significantly higher than that in Y and S (P = 0.0002, Figure 3A), meanwhile, the Chao1 index in TWA was significantly higher than S (P = 0.043, Figure 3B) as well, which indicated that the gut microbiota communities in TWA were more plentiful in diversity. No significant differences in good coverage index (P = 0.167, Figure 3C) indicated that the samples of the bacterial community were fully sampled. The phylogenetic diversity metrics (PD whole tree) analysis (P = 0.017, Figure 3D) showed the gut flora of S and Y had a closer evolutionary relationship, whereas, TWA had obvious community differentiation. Moreover, the beta diversity analysis by using PCoA (Figure 3E) and the homogeneity of dispersions test (Figure 3F) revealed that the composition of the gut prokaryotic community among the TWA, Y, and S were significantly different, and the gut microbiota structure of TWA differs from that of domestic animals.

SCFAs Profiles and Their Correlations With Gut Microbiota Communities
We surveyed two dominant SCFAs, and the results showed that both the concentration of acetate acid ( Figure 4A) and propionate acid (Figure 4B) in the fecal of TWA were significantly higher than S (P < 0.001), and the propionic acid concentration was significantly higher than Y as well (P < 0.05).
These results were consistent with PCoA analysis, in which the SCFAs profile in TWA had a trend to congregate with Y, on the contrary, separated with S ( Figure 4C). FIGURE 7 | Gut microbiota composition, and functional microbiota relate to cellulose degradation and methane production. Violin plot showing relative abundances of bacteria (A) and archaea (B) in different hosts. Analysis of relative abundances of cellulose-degrading species (C) and methane-producing archaea (D). Different letters denote statistically significant differences at corrected P < 0.05.
To further explore the driving factors affecting the production of SCFAs, we performed a correlation analysis using the indicator species phyla and genera as the driving factors and SCFAs and bacterial alpha diversity as the environmental factors. In the S group, Actinobacteria have positive effects on propionic acid production, and bacterial community diversity was affected by Planctomycete ( Figure 4D). In the TWA group, the relative abundance of Actinobacteria, Bacteroidetes, and genus community has positive effects on propionic acid production, and Simpson indices was affected by Bacteroidetes ( Figure 4E). In the Y group, Bacteroidetes had positive effects on propionic production, and both Firmicutes and genus have significant positive effects on bacterial richness ( Figure 4F).

Gut Enterotypes and Functional Contexts of Treponema 2, WCHB1-41, and Prevotella 1 in the Adaptation to Sparse Vegetation
Different enterotypes may functionally differ (Costea et al., 2018). Therefore, we investigated whether the gut microbiota partition into clusters with functional properties that vary among hosts. A principal component analysis (PCA) revealed that samples formed two distinct enterotype clusters based on their BC dissimilarities. The intestinal microflora of Y and S belonged to the enterotype1 clusters whereas the intestinal microflora of TWA belonged to the enterotype2 clusters. The clusters were distinguished by the relative differences in their representative bacterial genera. Akkermansia, Ruminococcaceae_UCG_010, Bacteroidetes, and Ruminococcaceae_UCG_005 were in enterotype1 while Treponema 2, WCHB1-41, and Prevotella 1 were in enterotype2 ( Figure 5A). The top 10 relative abundance of representative bacterial genera ( Figure 5B) of Ruminococcus_UCG_005, Ruminococcaceae_UCG_010, Akkermansia, Ruminococcaceae_UCG_013, and Bacteroidetes had significantly higher enrichment in enterotype1 (P < 0.001; Figures 5C-H, Supplementary Figure 3). In contrast, the relative abundance of Bacteroides_unclassified, WCHB1-41, Treponema 2, and Prevotella _1 were significantly higher in enterotrpe 2 (P < 0.001).
The distributions of the gut enterotypes in these hosts led us to hypothesize that the fixed enterotype represented by Treponema 2, WCHB1-41, and Prevotella 1 regulates the assimilation of essential nutrients from sparse forage. To test the hypothesis, we queried the functional relevance of Treponema 2, Prevotella_1, and WCHB1-41 in the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Treponema 2, WCHB1-41, and Prevotella_1 showed convergent enrichment of the enzymes involved in the arginine (Arg; map: 00220) and fatty acid (FA) biosynthesis (map: 00061) pathways (Figure 6). Pyruvate oxidation and decarboxylation to acetyl CoA are mediated by Treponema 2, Eubacterium, and WCHB1-41, and they enter the citrate cycle, promote Arg biosynthesis, and regulate FA biosynthesis. We detected 14 enzymes involved in Arg biosynthesis but no enzyme regulating urea synthesis. This physiological configuration mitigated urinary N loss in TWA under low-N stress. Seven of the eight foregoing enzymes directly participated in FA biosynthesis and energy generation. The preceding results suggested that both the Arg and FA biosynthesis pathways evolved in the gut of TWA grazing on sparse vegetation to ensure that they utilize N and generate energy under these conditions.

Meta-Genomic Sequencing Profile and the Functional Microbita Related to Cellulose Degrading and Methane Producing
Sixteen samples were selected (TWA, n = 7; Y, n = 4; S, n = 5) based on their β-diversity and Bray-Curtis (BC) distance values (Figure 3E) for meta-genomic sequencing. About 12.40 G clean bases per sample (Supplementary Table 5), with an average N50 length of 1.47 kb, including 14.93 million nonredundant unigenes and an average ORF length of 653 bp. A total of 3,766,521 genes were cataloged in the NR database annotation. The overall and domain-, phylum-, genus-, and species-level annotation rates were 80. 75, 75.38, 49.38, and 34.31%, respectively (Supplementary Table 6).
The bacterial abundance was significantly higher (P = 0.003) in the gut of TWA than it was in those of S and Y ( Figure 7A). By contrast, the abundance of archaea was significantly lower (P = 0.001) in the gut of TWA than it was in those of S and Y (Figure 7B). We then analyzed gut microbiota related to cellulose decomposition and methane production. The FIGURE 9 | Relative abundances of top12 genes encoding glycoside hydrolases (GHs), glycosyltransferases (GTs), carbohydrate esterases (CEs), and carbohydrate-binding moudles (CBMs). *P < 0.05, ** P < 0.01. abundances of the cellulolytic bacteria Prevotella ruminicola, Ruminococcus flavefaciens, Ruminococcus albus, Butyrivibrio fibrisolvens, and Ruminobacter amylophilus were significantly higher in the gut of TWA than they were in those of Y and S ( Figure 7C, Supplementary Table 7). Methanobrevibacter and Methanosphaera were the predominant methanogenic archaea in all samples, but their abundances were significantly higher in TWA than Y or S (Figure 7D, Supplementary Table 7).

Carbohydrate-Metabolizing Genes
To establish the potential capacity of gut bacteria to degrade herbage biomass, we sought carbohydrate-active enzymes (CAZymes) among the nonredundant genes. A hierarchically clustered heatmap (Figure 8) based on the overall CAZyme families disclosed that the unigenes in S and Y clustered together and separated from those in TWA. The compositions of the unigenes in Y and S resembled each other more closely than those of the unigenes in TWA.
To further explore the significance of these unigenes, a metastat analysis identified the underlying CAZyme families and the EC numbers (top 12 in terms of relative abundance) for the constituents differing among animal groups. In addition to GT2, the CAZymes GH43, GH3, GH31, GH5, and GH10 associated with cellulose and hemicellulose degradation were significantly more abundant in TWA than Y or S. Conversely, GH13, GH20, CE1, GT35, and GT4 related to starch and carbohydrate ester decomposition were significantly less abundant (P < 0.05) in TWA than Y or S (Figure 9, Supplementary Table 8). All the EC numbers in TWA were significantly higher than those in Y and S (P < 0.05; Supplementary Figure 4).

DISCUSSION
The low temperatures and hypoxia of the QTP make it an extremely harsh environment for mammalian species. Y, S, and TWA are primary consumers of alpine grassland. They graze the QTP grasslands all year and must contend with the severe challenges of extreme cold and limited food availability. Especially for TWA, they receive no special care such as forage supplementation from herdsman (Liu et al., 2019) and must subsist on sparse native forage. In the present study, we considered the nutrition and apparent digestibility of forage and cellulose activity in three co-resident herbivore species. In general, monogastric animals less effectively digest and assimilate high-fiber, low-protein grass than ruminants such as Y and S (Hintz et al., 1978). The high apparent digestibility of herbage is one of the vital factors for Y and S to adapt to the long cold season (∼6 months) and seasonal imbalance of pasture production of QTP (Dong et al., 2006). Similarly, forage digestibility of TWA determines the host's ability to cope with the nutrient stress of forage. In the present study, the higher apparent digestibility of TWA in CP (81.26%), NDF (65.14%), ADF (54.63%), and cellulose activity (517.79 U/g) revealed that TWA had a relatively more efficient nutrient uptake and could, therefore, withstand harsher nutritional stress than the domestic animal.
Host forage digestibility depends on the synergistic action of various gut microbiota (Brune and Ohkuma, 2010;Gomez et al., 2021). Host genome, phylogeny, diet, and environmental heterogeneity are usually the main factors affecting gut microbial community structure (Grieneisen et al., 2019). In our study, Y and S are the different species from different genera; however, there was no significant difference in their gut microbial diversity. This indicated that host genetics may not be a decisive factor influencing gut microbiota composition (Martinson et al., 2017). Host phylogeny can influence the microbial community diversity through immune-related genes that shape host-microbita interactions (Zhang et al., 2013. In our study, the experimental animals belonged to different phylogenies, and thus the different gut microbiota diversity and community structure might be related to this. However, based on a previous study of gut microbiota of captive colobine monkey species, researchers found that colobine phylogenetic relationships was not reflected in the gut microbiota (Vanessa et al., 2017); therefore, we speculated that phylogeny may not be a decisive factor as well. Of course, the structure and composition of gut microbiota were affected by multiple factors. In our study, the relatively higher gut microbiota diversity observed in TWA might be explained by environmental heterogeneity. Y and S were under the supervision of a professional shepherd and subsisted on high-quality grass pastures. In contrast, human interference forced TWA to travel longer distances to eat lower-quality indigenous plants in various habitats. Cao et al. (2009) reported that during the warm season, 16 plant species were edible for Y and S, while 19 plant species were edible for TWA. Differences in grazing strategy lead to differences in environmental heterogeneity. For wild animals, wide feeding ranges are beneficial for the diversification of food and gut microbial diversity. Conversely, domestic animals grazing under the control of herdsman are subject to reduced environment heterogeneity, which is not conducive for the development of microbial diversity. Thus, environment heterogeneity and food source diversity may be complementary. Therefore, we believed that diet and environmental heterogeneity significantly influence the differences between domestic and wild animals in terms of gut microbial diversity. The results of the present study were consistent with an earlier one indicating that among host behavior, geographical distance, environmental variation, and genetics, environmental variation was the principal predictor of host-microbiota associations (Song et al., 2020).
In this study, we systematically and comprehensively analyzed the differences in the gut microbiota of wild and domestic animals. Similar to previous studies (Liu et al., 2020b;Jiang et al., 2021), Bacteroidetes and Firmicutes, which were related to cellulose and hemicellulose decomposing, were found to be the most abundant bacterial phyla in our study. Moreover, indicator analysis showed these two phyla were represented by Y and TWA. The fact that Y possessed the potential to tolerate roughage through numerous Bacteroidetes consortiums has been reported (Guo et al., 2021). Here, the presence of Bacteroidetes indicator species in TWA suggests that these animals might be more tolerant to roughage than Y. This speculation is supported by the relatively higher herbage ADF and NDF digestibility and cellulose activity in the gut of TWA.
The SCFAs, the metabolites of forge fermentation by microorganisms, are important energy sources for the host, and they also maintain the homeostasis of the intestine (Popkes and Valenzano, 2020). Studies have shown that the main SCFAs in the gut are acetate and propionate (Fan et al., 2020). Consequently, we designated them as the main environmental factors and elucidated their relationships with microbial community indicator species. We found Actinobacteria were significantly positively correlated with propionate production in the gut of S while Planctomycetes was significantly positively correlated with the Shannon and Simpson indices. The foregoing bacteria did not predominate in the intestinal microflora. Nevertheless, they increased gut microbiota diversity and homogeneity and may have helped S to digest and absorb dietary nutrients. In TWA, all indicator species in the Bacteroidetes and Actinobacteria (but not Kiritimatiellaeota), as well as the genera Prevotella_1 and Rikenellaceae promoted propionate production. For Y, however, only Firmicutes positively affected propionate production. As TWA had a diverse gut microbial consortium associated with propionic acid production, we speculated that this wild herbivore absorbed energy more effectively than the domestic herbivores (van Kessel et al., 2011). This advantage enabled TWA to cope with harsh environmental conditions and food scarcity.
Enterotypes were first discovered in the human gut microbiome and are roughly divided into Bacteroidetes, Prevotella, and Ruminococcus. These three taxa vary widely in terms of energy processing, vitamin biosynthesis and, by extension, impact host health (Arumugam et al., 2011). Guo et al. (2021) reported that yak shifted their enterotypes in response to dietary changes between the warm and cold seasons so that they could utilize N and energy most effectively. Fan et al. (2020) revealed the formation of various enterotypes helped QTP pika adapt to complex food conditions. The present study provided biological insight into gut enterotype clusters and functions as well as functional genomic information for wild and domestic herbivores. The Akkermansia, Ruminococcaceae sp, and Bacteroides enterotype which associated with high protein and low fiber forage nutrition belonged to Y and S, whereas, the Prevotella_1, WCHB1-41, and Treponema 2 enterotype with low protein and high fiber belonging to TWA. It was suggested that the proportion of protein and carbohydrate contents mediated the host's enterotype shift, at least in wild baboons (Ren et al., 2016) and domestic yak (Guo et al., 2021). In our study, the variations in dietary protein and fiber contents could provide an attractive explanation for the differentiation of enterotype, and might also contribute to determine the enterotype for highaltitude herbivores on QTP. Y and S are indigenous livestock grouped into a single enterotype. This finding is an evidence for the existence of convergent gut microbe evolution (Xue et al., 2017). The formation of the TWA intestinal type might have been the result of long-term co-evolution between the host and the environment. It suggests that fixed enterotypes play key roles in animal adaptation to extreme environment. The present study also provided a mechanistic explanation as Treponema 2, WCHB1-41, and Prevotella_1 participated in Arg and FA biosynthesis to utilize dietary N and generate energy for TWA subjected to harsh forage and environmental conditions. During the cold season on the QTP, domestic animals such as Y have lower N requirements and utilize dietary N more efficiently than cattle (Guo et al., 2012(Guo et al., , 2021Zhou et al., 2017). In the context of a growing overlap between the living space of wild and domestic animals, our study clarified the influences of Treponema 2, WCHB1-41, and Prevotella_1 enterotypes on N and energy utilization in TWA and showed that these enterotypes play vital roles in mediating nutrient homeostasis in wild animals native to high altitude.
As 16S amplicon sequencing is limited (Qin et al., 2010), we elucidated gut microbiota structure and function via metagenomic sequencing. The compositions of bacteria and archaea differed significantly among Y, S, and TWA. Bacteria were enriched in the TWA gut while archaea were enriched in the intestines of domestic animals. Certain grazing animals do not digest grass per se. Rather, they assimilate the bacteria decomposing the plant biomass into various nutrients (Niu et al., 2015). In the present study, we found that cellulolytic and proteolytic bacteria (Hastie et al., 2008;Liu et al., 2020a) such as R. flavefaciens, P. ruminicola, B. fibrisolvens, R. albus, R. amylophilus, and F. succinogenes were significantly more abundant in TWA than Y or S. These findings were consistent with the fact that TWA had relatively higher DM digestibility. Therefore, we concluded that the comparatively higher abundance of functional bacteria in the gut of TWA improves forage digestibility and produces high SCFAs concentrations to help the host efficiently use the forage, obtain energy, and adapt to environments with relatively less or inferior forage. When H 2 accumulates during bacterial catabolism, archaeal growth is stimulated by H 2 incorporation into greenhouse gases (GHG) such as methane and carbon dioxide (Matarazzo et al., 2012;Hoffmann et al., 2013). In the present study, Methanobrevibacter, Methanosphaera, and Methanobacteriun (Shi et al., 2014) associated with methane production were enriched in the intestinal tracts of S and Y. The methane emission from herbivorous not only results in environmental problems but also represents a significant source of energy loss to animals (Lan and Yang, 2019). A previous study revealed that Y and S were sustainable high-altitude mammals as their unique microbiome genes and structures could aid in the biological control of GHG emissions (Zhang et al., 2016). Here, we did not perform laboratory-level gas production assays on TWA, Y, or S from the perspective of the gut microbiome functional analysis; however, TWA might be more sustainable than Y or S as the former have lower methane emission potential than their domestic animal co-residents and might use energy more efficiently to cope with the perennial cold climate of the QTP.
The capability of herbivores to transform plant biomass into fermentable sugars depends entirely on the polysaccharidehydrolyzing enzymes produced by intestinal microflora (Bohra et al., 2019). β-Glucosidase (EC 3.2.1.21) degrades plant cellulose by hydrolyzing the terminal non-reducing β-D-glucose bond (Xiros et al., 2013). Arabinanase (EC 3.2.1.99), xylanase (EC 3.2.1.8), and β-xylosidase (EC 3.2.1.37) synergistically degrade hemicellulose in plant cell walls (Sunna and Antranikian, 1997). The carbohydrate-active enzymes database (CAZy) provides information on CAZyme families including GHs, GTs, PLs, CEs, CBMs, and AAs. Of these, GHs are the most diverse and abundant (Cantarel et al., 2009). In the present study, we described the top 12 CAZyme families and enzymes. GH43, GH3, GH31, GH5, and GH10 decompose cellulose and hemicellulose and were more abundant in TWA than S or Y. Conversely, enzymes digesting starch and carbohydrate esters were comparatively more enriched in domestic animals. These discrepancies may be explained by the differences in the host feeding niches. TWA usually feeds on forage grass with high cellulose content whereas domestic animals selectively consume high-quality forage under the intervention of herdsmen. Convergent evolution of the functional genes of intestinal flora is also the consequence of host adaptation.

CONCLUSION
The feeding niche heterogeneity is one of the main factors leading to the differences in gut microbiota among Y, S, and TWA. As the native herbivorous domestic livestock, the gut microbiota of Y and S exhibit convergent evolution characterized by the similar community and diversity and the same enterotype. Moreover, the intestinal flora of bath animals have an advantage in degrading plant starch and esterase. The indigenous TWA are highly adapted to the low temperatures, hypoxia, and highfiber, low-protein native vegetation of this harsh environment in part because the composition, structure, and diversity of their gut microbiota are distinct from those of their domestic livestock co-residents. Furthermore, the intestinal microflora of TWA is characterized by high diversity, high nitrogen utilization efficiency and energy generation, abundant cellulolytic bacteria, high enzyme activity, and numerous gene families encoding glycoside hydrolases. The present study revealed the unique gut microbiota-mediated mechanisms by which TWA adapts to abiotic stress and poor forage quality. It also provides an important theoretical basis for the protection of wild animals indigenous to the QTP and the development and therapeutic application of intestinal microflora.

DATA AVAILABILITY STATEMENT
The raw sequence data reported in this paper have been deposited in Genome Sequence Archive (Wang et al., 2017) in BIG (BIG Data Center Members, 2019), Beijing Institute of Genomics (BIG), Chinese Academy of Sciences, under the accession numbers CRA006663615 (http://bigd.big.ac.cn/gsa).

ETHICS STATEMENT
The animal study was reviewed and approved by Northwest Institute of Plateau Biology, CAS-Institutional Animal Care and Use Committee (OGRD# 2016YFC0501905).