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Shift to emergency remote preclinical medical education amidst the Covid‐19 pandemic: A single‐institution study

Abstract The Covid‐19 pandemic disrupted medical education, shifting it towards emergency remote delivery. This cross‐sectional study aimed to assess the impact of the pandemic on preclinical medical education and identify predictors of the virtual learning experience and perceived stress. An anonymous survey was delivered electronically to the students of the authors' medical school that attended either histology or pathology. This survey contained two scales, the virtual learning experience (VLE) and the perceived stress scale‐10 (PSS‐10). A total of 173/255 (68%) responded, showing a positive perception towards the remote delivery of both courses. An exploratory factor analysis was performed on the VLE scale items and four new dimensions were formed: “course quality and learning outcomes”, “student motivation”, virtual against F2F learning”, and “virtual laboratory sessions”. The following significant predictors of enhanced VLE, in at least one dimension, were identified: female gender, pathology course, final examination grade >80%, lower perceived stress levels, studying in home country, and holding of another degree before medical school. In addition, the following predictors were significantly associated with higher levels of student perceived stress: female gender, pathology course, studying away from home, and suboptimal internet connection. Notably, the quality of internet connection was significantly associated with the students' final examination performance. Concerning the best mode for future delivery of both courses, most students proposed a blended, rather than an entirely on‐campus or online approach. In conclusion, despite its problems, a high‐quality remote preclinical medical education was possible in the authors' school and offered tremendous opportunities for future improvement.

schools around the globe closed their premises overnight, while face-to-face delivery was halted and replaced by emergency remote teaching both for preclinical and clinical courses (Cuschieri & Calleja Agius, 2020;Longhurst et al., 2020;Torda, 2020).
This abrupt change posed numerous challenges for medical educators, who had to deal with the students' loss of hands-on experience (Franchi, 2020;Patra et al., 2021), lack of interaction and engagement, in addition to generating new teaching resources for virtual teaching, since transferring the same material and teaching strategies used for F2F teaching would be unsuitable (Evans et al., 2020). Technical limitations, such us the uneven access to digital technology or unreliable internet connection, could also hamper virtual delivery of the courses (Dhawan, 2020). In addition, the pandemic, with its' associated high level of threat, increased the students' anxiety and stress levels (Reuman et al., 2015;Saraswathi et al., 2020;Taylor et al., 2020b). Factors linked with the worsening mental health included the shift to online class delivery, isolation from peers, financial issues, and others (O'Byrne et al., 2021), while students with preexisting mental disease exhibited higher levels of stress during this period (Guo et al., 2021).
Despite the abovementioned challenges, this new situation gave educators the opportunity to use technology to enhance learning experience (Kirkwood & Price, 2014), develop novel teaching resources (Pather et al., 2020), combine synchronous and asynchronous learning, engage students, and support activities that enhance networking (Rhim & Han, 2020). Strategies applied included the development of real-life online case scenarios (Rahm et al., 2021), the use of "flipped classroom" (Viveka et al., 2017;El Sadik & Al Abdulmonem, 2021;Qian et al., 2021), and social media (Flynn et al., 2015;Katz & Nandi, 2021), aiming to enhance student motivation and improve learning outcomes. Notably, student mentoring programs were re-organized to be conducted virtually through online platforms, and even the social media with the help of their senior peers (Kazerooni et al., 2020;Zibold et al., 2021) In the field of anatomical sciences, although the pandemic caused a decline in the use of cadavers and other hands-on laboratory modalities (Franchi, 2020;Patra et al., 2021), it multiplied virtual teaching resources and facilitated hybrid (synchronous and asynchronous) activities (Harmon et al., 2021;Harrell et al., 2021). For the laboratory instruction of histology and pathology, medical educators relied on virtual microscopy (VM), which is more convenient and does not hamper academic performance compared to light microscopy (Somera Dos Santos et al., 2021). Notably, several authors have shown that VM enhances student active participation and examination scores (Krippendorf & Lough, 2005;Selvig et al., 2015;Yohannan et al., 2019;Lee et al., 2020). Besides enhancing learning and test performance, a study showed VM, when used together with light microscopy (LM), resulted in more excellent and fewer failing scores in histology and pathology, also less score variability, compared to the LM only group (Lee et al., 2020). However, VM requires high-speed internet connectivity with enough bandwidth, as microscopic image files have a large size, to allow optimal user experience (Evans et al., 2020), while, on the other hand, students have expressed concerns about lacking the skill to operate the light microscope (Somera Dos Santos et al., 2021). In a survey involving a few Chinese medical schools during the pandemic, histology educators responded that they relied to a great extent on VM, active learning, and online assessments (Cheng et al., 2021). Furthermore, in an Australian medical school, online laboratory sessions coupled with VM resulted in enhanced practical examination scores compared to the previous year, when F2F teaching was applied (Caruso, 2021). Of interest, pathology educators in the United States found the opportunity to establish virtual pathology electives; these had high enrollment rates, and were rated favorably by the students; the latter believed they understand the importance of pathology to modern medical practice and consider a potential future career in pathology to a greater extent that the previous years (Parker et al., 2020;Fu et al., 2021).
Shortly after the pandemic onset, the authors' medical school responded rapidly by re-structuring its program to be delivered remotely, allowing many of its students to return to their home countries. Virtual teaching was applied to all preclinical medical courses, including histology and pathology. As many faculty members and students were new to online education, the school's IT department supported them by organizing training sessions, maintaining the Blackboard Ultra learning management system (Blackboard Inc., Washington, DC), which hosted the school courses, and answering questions through a troubleshooting line.
As the Covid-19 pandemic triggered unparalleled changes in medical education, this exploratory study aimed to: (1) Present the student perceptions on preclinical emergency remote teaching, focusing on the histology and pathology courses; (2) Identify predictors of the virtual learning experience; and (3) Identify predictors of perceived stress.

Medical school curriculum
The six-year MD program follows a spiral, competency-based curriculum ( Figure 1) that allows horizontal and vertical integration of knowledge (Masters & Gibbs, 2007;Bandaranayake, 2017). A "structure and function" (S&F) approach of each body system is followed through the studies, while student-centered strategies are applied such as team and problem-based learning and peer-teaching (Allchin, 2013;Karamaroudis et al., 2020). The curriculum is composed of three phases. Phase I (Foundations of Medicine; Years 1-2) comprises two stages, "S&F: from Molecules to Cells" and "S&F: Body Systems in Health", and introduces students the normal human body structure and function, also with skills such as history taking, teamwork, and professionalism. Phase II (Foundations of Clinical Practice; Year 3) consists of "S&F: Body Systems in Disease I" (Semester 5) and "S&F: Body Systems in Disease II" (Semester 6); this phase aims to familiarize students with the abnormal human body structure and function, besides physical examination, clinical skills and reasoning. Phases I and II are preclinical and mainly take place in the medical school campus. Basic and advanced skills development is mostly performed in the simulation training complex, which includes high-fidelity, procedural skills, and simulated outpatient consulting rooms, in addition to a standardized hospital training suite. Lastly, phase III (Clinical Medicine Core) brings the students to the teaching hospitals to apply and gradually built on the concepts learnt during the Phases I and II.

Histology and pathology courses description
Histology and Pathology are disciplines that rely on the distinction and interpretation of visual information regarding structure.
Histology is taught during the Phase I of the school curriculum ("Histology-Embryology I" in semester 2; "Histology-Embryology II" in semester 3), whereas Pathology during the Phase II ("Pathology I" in semester 5; "Pathology II" in semester 6). Weekly activities for both courses include two to three hours of lectures and three to four hours of laboratory sessions (six hours per course for 13 weeks each semester). The same faculty members (C.M., S.C.T., I.P.N.) are involved in the teaching of both histology and pathology courses.
Laboratory sessions are conducted in a similar fashion; they start with a description of static microscopic images while integrating structural, functional, and clinical associations, continue with the examination of unknown microscopic slides, and conclude with discussion of one or a few longer cases with clinical relevance. Lectures and laboratory sessions are designed to be interactive and promote active learning by increasing complexity in a stepwise manner (Tian et al., 2014). All student handouts are available prior to the sessions onto the Blackboard Ultra learning management system (Blackboard Inc., Washington, DC). Independent learning includes working on a weekly, tutor-marked, laboratory assignment that highlights laboratory-clinical integrations and completing a short multiple-choice question (MCQ) quiz, with immediate feedback, through Google Forms (Google LLC., Mountain View, CA). The latter revises the knowledge acquired during the lectures and laboratory sessions of each module. Recommended resources include textbooks e.g., Junqueira's Basic Histology: Text and Atlas (Mescher, 2021), Robbins Basic Pathology (Kumar et al., 2017), websites e.g., Michigan Histology and Virtual Microscopy Learning Resources (UMMS, 2021), and YouTube Videos. Online resources become F I G U R E 1 The European University Cyprus medical school follows a spiral, competency-based curriculum. It consists of three Phases, while a "structure and function" (S&F) approach of each body system is followed through the studies. Histology is taught during the Phase I of the school curriculum ("Histology-Embryology I" in semester 2; "Histology-Embryology II" in semester 3), whereas Pathology during the Phase II ("Pathology I" in semester 5; "Pathology II" in semester 6) readily available to all students before the sessions begin through weekly laboratory plans. Assessment in both courses takes into account the students' performance on a midterm examination, a final examination, the weekly assignments, and class participation. Each examination consists of 50-60 board style MCQs with and without microscopic images and include questions of diverse difficulty levels, according to Bloom's Taxonomy (Zaidi et al., 2017).
Before the pandemic, all lectures and laboratory sessions were performed F2F on-campus, while students used a combination of light and virtual microscopy to examine microscopic slides ( Figure 2).
Sessions were not recorded. However, the pandemic forced an overnight transition to emergency remote teaching ( Figure 2). All sessions kept being delivered synchronously, albeit virtually through Blackboard Collaborate (Blackboard Inc., Washington, DC). Virtual microscopy was solely used in the laboratory exercises, a shift not difficult to implement as teaching resources were already prepared before the pandemic. Instructors were able to share their screen and explain microscopic structures at diverse magnifications. Students were allowed to and encouraged to actively engage by using the microphone, chat function, or polling, all of which were available on the platform. The latter also facilitated the tracking of each student's progress. Weekly assignments were performed asynchronously with a submission deadline until the end of the week. Notably, recordings were made readily available some minutes after the completion of each session, offering students flexibility to re-visit the material. E-mentoring was provided remotely through the same platform.
Lastly, midterm and final examination assessments took place remotely, using online proctoring through the Respondus Lockdown Browser (Respondus Inc., Redmond, WA).

Bioethics committee approval
The Cyprus National Bioethics Committee approved the protocol of this research (reference number: 2020.01.139; 24 June 2020). The aim of the survey, besides the fact it was anonymous and optional, were explained to the students before consenting to participate.

Survey details
After the completion of the 2020 spring semester (Figure 2), an e-survey (Table S1) was sent to the first and third-year students of the authors' medical school, as well as the second-year students of the biomedical sciences program. The semester started with on-campus teaching for a few weeks, yet rapidly shifted towards remote delivery ( Figure 2). Histology participants (Histology-Embryology I; Figure 1) were attending their F I G U R E 2 Timeline showing the transition to emergency remote teaching in the authors' medical school, during the 2020 spring semester, and the delivery of the survey to the students, after the semester finished. Similarities and differences in the learning environment of Histology and Pathology courses, before and after the transition, are also presented first microanatomy-oriented course with only a few weeks of on-campus experience, before the pandemic hits. In contrast, pathology students (Pathology II; Figure 1) had already completed two conventional courses in histology (Histology-Embryology I and II) and one in pathology (Pathology I). The same faculty members (C.M., S.C.T., I.P.N.) were involved in the teaching of both histology and pathology courses.
The survey was anonymous and optional and required around 10 min to complete. It was delivered electronically via Google Forms (Google LLC, Mountain View, CA) and comprised background information questions, two scales composed of Likert items, and a few open-ended questions ( Table S1). Examples of background information questions included students' gender, first language, place of living during remote delivery of the classes, familiarity with technology, quality of internet connection, and final examination grade. Students were asked to provide their grade within pre-defined ranges (<60%; 60%-70%; 70%-80%; 80%-90%; 90%-100%). The first scale was created by the authors, aiming to collect the students' perceptions on their virtual learning experience (VLE scale) while attending the histology and pathology virtual classes. The VLE scale (7-point scale; strongly disagree-strongly agree) was composed of 20 Likert items (Figure 3), questioning the quality of the teaching and its comparison with the pre-pandemic era, the students' perceptions on the importance of these courses, their motivation, and their confidence in the skills they obtained.
The second scale used was the perceived stress scale-10 (PSS-10), defined by Cohen et al. (1983) and Cohen and Williamson (1988).
The survey was open for responses for a four-week period ( Figure 2). Students were notified to participate with weekly emails, also with posts in their WhatsApp year groups by their year student representatives, and their answers were received by the deadline.
Data were exported into an Excel program (Microsoft Corporation, Redmond, WA) before processing.

Statistical analysis
Cronbach's a and Kendall's tau B (Tables S2 and S3) were used to assess the reliability of both VLE and PSS-10 scales of the survey. Categorical variables were compared with the Pearson's chisquared test, with results shown as frequencies and percentages.
Continuous variables were compared with the independent samples t-test for normally distributed variables or the Mann-Whitney test for non-normally distributed variables, with results shown as means with ±standard deviation (±SD) or medians with interquartile range (IQR). To investigate the construct validity of the VLE questionnaire, an exploratory factor analysis with principal axis factoring was performed and the rotation method used was Varimax with Kaiser Normalization. To confirm that factor analysis was appropriate for the data, we computed the Kaiser-Meyer-Olkin measure of sampling adequacy and the Bartlett's test of sphericity. The regression method was used to compute the factor scores of the VLE dimensions resulted from the exploratory factor analysis. The independent samples t-test was used to compare the factor scores of the VLE dimensions and the Cohens' d effect size was calculated to characterize the strength of the association between VLE dimensions and possible predictors. Lastly, multivariable linear regression models were used to identify significant predictors of VLE and perceived stress, respectively. Multivariable linear regression models include simultaneously several predictor variables in the same model and compute the independent and adjusted effect of each predictor variable to the outcome variable. The standardized regression coefficients of the multivariable linear regression models were the effect sizes of the predictor variables. Statistical analysis was performed with the SPSS statistical package, version 26 (IBM Corp., Armonk, NY) and R statistical software, version 2.12.1 (R Foundation for Statistical Computing, Vienna, Austria). Significance test and confidence intervals were calculated at significance levels of P = 0.05.

RE SULTS
Both survey scales were found to have a good reliability. Cronbach's a for the VLE scale was 0.85, after reverse-coding the negatively keyed items 4, 17, 19, and 20 (Table S1) Among the survey participants, the quality of internet connection was significantly associated with the students' final examination performance (Table 1; P = 0.04). Females, students of first language other than English or Greek, holders of another degree prior to medical school, students that attended online classes in their home country, students living with someone else (e.g., their family or a fellow student), and expert/advanced technology users achieved higher scores, but differences were not statistically significant (Table 1).
Lastly, when participants were asked about their perception for the best mode of histology and pathology course delivery in the future, most students favored some form of blended learning (57.5% in histology; 67.5% in pathology), while fewer favored entirely oncampus (38.8% in histology; 27.5% in pathology) or entirely online delivery (3.8% in histology; 5.0% in pathology) (Table 1).

Student perceptions on preclinical emergency remote teaching
Remote delivery of both histology and pathology courses was wellperceived by the students (Figure 3 and Table S4). In a Likert scale with answers ranging from 1 to 7 (strongly disagree-strongly agree), participants showed a very positive perception towards the importance of both courses for understanding disease (mean: 6.38 ± 0.91) and their future career as physicians (mean: 6.14 ± 1.09), their remote delivery during the Covid-19 pandemic (mean: 6.36 ± 1.01), the quality of accompanying resources such as online quizzes or videos (mean: 5.79 ± 1.40), the encouragement of their participation (mean: 5.81 ± 1.39), and the fairness and objectivity of online examinations (mean: 5.78 ± 1.66).
Of interest, students' answers on the negatively keyed items, which compared virtual with traditional F2F instruction, had medians that ranged between 3 (somewhat disagree) to 4 (neither agree nor disagree) (Table S4). More specifically, their answers in the following statements-"It was more difficult to actively participate (e.g., ask questions) in the online rather than the on-campus sessions" [median: 4 (4.0)], "Studying for the online sessions was much harder than the campus-based sessions" [median: 4 (4.0)], "I would have learned more if this course was entirely taught on-campus rather than online" [median: 4 (4.0)], and "During the Covid-19 pandemic, it was harder for me to access this course instructor for questions compared to the period the campus was open" [median: 3 (4.0)]-is another sign they perceived favorably their remote preclinical education in these particular two courses.

Predictors of virtual learning experience
To give validity on the evidence derived from the VLE scale data, an exploratory factor analysis was performed. The Kaiser-Olkin measure of sampling adequacy was equal to 0.83, while the P-value of the Bartlett's test of sphericity equal to 0.001. Four dimensions were formed as a result of this analysis (Table 2): F I G U R E 3 Histology and Pathology students' virtual learning experience (VLE) in the authors' medical school. Results of each VLE item are presented as means with ±standard deviation (±SD). Data were obtained using seven-point Likert scale (1 = strongly disagree; 2 = disagree; 3 = somewhat disagree; 4 = neither agree nor disagree; 5 = somewhat agree; 6 = agree; 7 = strongly agree) TA B L E 1 Background characteristics of the students participating in the survey  The tool used to assess the variable "Perceived Stress" was the perceived stress scale-10 (PSS-10), which is composed of 10 five-point Likert scale items where 0 = never and 4 = very often and its processing gives scores ranging from 0 to 40 [0-13 (low stress); 14-26 (moderate stress); 27-40 (severe stress)].
• Course quality and learning outcomes • Student motivation • Virtual against F2F learning • Virtual laboratory sessions Data were further interpreted using these dimensions (Table 3), also the VLE scale items individually (Table S4). Histology students perceived most dimensions of their VLE less favorably than pathology students (Table 3) (Table S4); the latter could be interpreted as a stronger need to re-check the materials after the delivery of each session.
In addition, students that scored less than 80% in their final examination also perceived their VLE less favorably than the ones scoring more than 80% (Table 3) (Table S4).
Subsequently, a multivariable linear regression model was formed to identify predictors of the four dimensions of the VLE scale (Table 4). Comparable with the previous analysis, the histology course also had a negative effect in three out of four VLE and "course quality and learning outcomes" [coefficient (95% CI):  To summarize, the following significant predictors of enhanced VLE, in at least one dimension, were identified in this analysis: female gender, pathology course, final examination grade >80%, lower perceived stress levels, studying in home country, and holding of another degree before medical school.

Predictors of perceived stress
To identify significant predictors of perceived stress, a multivariable linear regression model was created (Table 5). The following variables were found to have an independent effect on the student's perceived stress: gender, course, first language, place of attending online classes, and quality of internet connection. Male participants reported lower levels of stress than females (mean difference: In addition, students holding another degree prior to medicine, living with someone else rather than alone, being more familiar with technology, and obtaining a final examination grade >80% reported lower levels of stress, but the differences were not statistically significant (Table 5).

DISCUSS ION
Success of emergency remote teaching during the pandemic has been challenged by technical limitations, such as unreliable internet connection, mental fatigue, lack of engagement and hands-on experience of the students, besides the medical educators' inexperience teaching online or moderating successful virtual smallgroup teaching sessions (Cuschieri & Calleja Agius, 2020;Dhawan, 2020;Evans et al., 2020;Pather et al., 2020). However, despite its numerous challenges, this pandemic has given preclinical medical educators a tremendous opportunity to learn, experiment with new ways of learning, and integrate innovative technologies into their courses (Gaur et al., 2020;Parker et al., 2020;Samueli et al., 2020), advancing their leadership and digital skills (Smith & Pawlina, 2021).
This study showed the remote delivery of both histology and pathology courses in the authors' medical school was well-accepted by most students, who perceived the course outcomes were reached, despite the unprecedented changes induced by the pandemic.
Student engaged in the online sessions, making use of the tools provided by the platform that enhance interaction such as the microphone and chat function (Rivera et al., 2021). They also used VM to practice during the laboratory sessions. There are various free VM repositories designed for medical educators, including the Virtual Microscopy Database (Lee et al., 2018), the Iowa Virtual Slidebox (Dee, 2009), and the PathPresenter (Singh, 2021). Research has shown that using VM induces equivalent (Mione et al., 2013;Ordi et al., 2015) or superior learning outcomes (Kuo & Leo, 2019;Lee et al., 2020;Rodrigues-Fernandes et al., 2020) at the medical school level compared to light microscopy, while LM additionally offers significant advantages, such as enhanced accessibility and collaboration among students, superb image quality, and cost-effectiveness (Dee, 2009;Saco et al., 2016). Further supporting evidence comes from a recent meta-analysis, which has shown that e-learning results in superior learning outcomes in medical education (Pei & Wu, 2019).
This study also demonstrated that, although highly supportive, histology students perceived virtual education with more caution than pathology students. As a similar teaching methodology was employed and the same faculty were involved in the teaching of both courses, these results could be explained on the basis of experience and level of studies. While pathology students had already obtained experience from completing two histology and one pathology conventional courses, first year histology students were attending their first microanatomy-related course when the pandemic hit ( Figure 1). Thus, pathology students possibly found it easier to adjust to the new circumstances forced by the pandemic. In histology and pathology medical courses, images and slides are used as a means to solidify basic processes, understand disease, emphasize basic terminology and laboratory workflow, integrate with other disciplines, promote independent learning and thinking, and educate future physicians of any specialty, rather than train at the level needed for basic scientists or first year pathology residents (Weston, 2018;Parker et al., 2020). These concepts could have been more evident to pathology students, who felt less alarmed by the lack of hands-on TA B L E 5 Predictors of the students' perceived stress Note: The tool used to assess the students' perceived stress was the perceived stress scale-10 (PSS-10), which is composed of 10 Likert items [5-point scale; 0 (never)-4 (very often)] and its processing gives scores ranging from 0 to 40. A total of 173 students (100 females; 73 males) participated in the survey and answered this particular tool. Significant results are given in Bold. a Multivariable linear regression model. optical microscopy exercises and its potential effect on the quality of their studies, than the histology students. A study by Holaday et al. showed that histology students exhibited a strong preference towards virtual rather than traditional approaches, while this preference increased as the course progressed (Holaday et al., 2013).
This study showed a similar trend, albeit during the progression from histology to pathology rather than the histology course itself.
High examination performance was significantly associated with student motivation and adjustment to virtual learning. Indeed, to succeed in online learning, students have to manage their time, plan and regulate their learning effectively, and be critical thinkers (Broadbent & Poon, 2015). Furthermore, our study revealed the quality of internet connection was significantly associated with the students' final examination performance. This is in accordance with the literature, where suboptimal internet connectivity has been shown an important barrier to successful virtual medical education (O'Doherty et al., 2018).
Notably, perceived stress, as defined by Cohen et al. (1983) and Cohen and Williamson (1988), was significantly associated with specific student characteristics. Females reported they experienced higher levels of stress, which is a finding also supported by other studies before (Stegers-Jager et al., 2020) or during the pandemic (Kannampallil et al., 2020;Salari et al., 2020;Taylor et al., 2020a;Wang et al., 2021). Compared to histology students, pathology students experienced more stress. A recent study reached similar result, reporting higher stress levels during the transitional third year of medical studies (Abdulghani et al., 2020). Indeed, at this stage, medical students are at the crossroads between the preclinical and clinical phase of their medical education. This brings them closer to the realization of the role they are to take at the end of their degree, which could increase their stress levels. Recent studies suggest that medical staff and trainees who have been exposed to patients with Covid-19 have experienced higher levels of stress and burnout (Kannampallil et al., 2020;Li et al., 2020;Wu et al., 2020).
Another study also pointed that residents experienced anxiety more frequently than medical students during this period . On the other hand, histology students have potentially not yet fully incorporated to the demands of their medical studies and profession and are still in the initial, and more care-free, adjustment phase. The higher stress levels experienced by those attended the semester away from home could possibly be due to loneliness or lack of support, while in students with native tongue other than Greek or English, it could be implied that cultural factors play a pivotal role.
Lastly, students with poor/average internet connection also experienced higher levels of stress at a significant level, as they could be concerned with an additional obstacle to the smooth and successful completion of their studies.
This study and others have shown that emergency remote teaching due to the pandemic has offered educators tremendous opportunities for innovative teaching. However, students lacked the access to the physical university environment, face-to-face interactions with faculty and peers, and hands-on education (Franchi, 2020;Patra et al., 2021). At this point, the main question that arises is what would be about the best delivery mode of histology and pathology courses (or preclinical medical education in general) in the near future, after the pandemic finishes. Could such models be viable in the long term or they were just a short-term solution? When the students were asked in this study, most proposed some form of blended instruction, rather than solely on-campus or online. In-class sessions could provide hands-on laboratory skills, interactions with peers and educators, professionalism, role-modeling, and effective teamwork (Smith & Pawlina, 2021). Online sessions could be ideal for introductory lectures and offer a flexible learning environment with innovative teaching solutions, facilitate self-directed and life-long learning, and supplement F2F instruction, enhancing students' knowledge acquisition and academic performance (Khalil et al., 2018;Chen et al., 2020;Caruso, 2021). According to the findings of this study, online sessions could be more effective at the late rather than early years of the preclinical medical education, without compromising the quality and lowering the academic standards. Of interest, supportive data come from a recent meta-analysis, which combined 56 studies from the whole spectrum of health education without a focus on anatomical sciences; this study showed that blended learning exhibits better knowledge outcomes compared to traditional education (Vallée et al., 2020). Therefore, a direction towards blended learning, which will mix traditional on-campus and online education keeping the best parts of both, could be the way to go in future preclinical medical education.

Limitations of the study
This study has some important limitations. It was a cross-sectional survey, retrospective in design, and prone to response bias. As it took place after the semester had finished, it was based on student recall. The survey questions were voluntary to complete and a few students did not give an answer to some of them, such as their final examination score. This was a research focused on student perceptions, while two different courses were compared, histology and pathology. Results were solely derived from histology and pathology courses, which means that VLE in other preclinical courses could differ. For example, a recent study about preclinical education revealed negative student perceptions (in contrast to this study) due to absence of hands-on learning, inability to learn clinical skills, mental exhaustion, and digital fatigue (Shahrvini et al., 2021). Additionally, results of this study came from a single institution. Lastly, there is a possibility of other variables (e.g., study habits) that were not assessed, which could potentially affect the study outcomes.

CON CLUS IONS
This cross-sectional study aimed to present the student perceptions on preclinical emergency remote teaching and identify predictors of the virtual learning experience and perceived stress. Despite some challenges, it appears that high quality preclinical medical education is possible via virtual learning. While the pandemic forced innovative changes in teaching approaches, histology and pathology online delivery was well-accepted by most students in the authors' medical school. Pathology students and students with high final examination scores perceived their virtual education more favorably. Lastly, significantly higher levels of stress were noted in females, also in students who attended pathology (rather than histology), had first language other than Greek or English, studied away from their home country, or had a suboptimal internet connection.

ACK N OWLED G M ENTS
The authors wish to thank the medical students at the European University Cyprus for participating in this survey and for their continuous support.

CO N FLI C T O F I NTE R E S T
The authors do not have any conflicts of interest to declare.