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Process control and design considerations for methanol‐induced denitrification in a sequencing batch reactor

Abstract The primary goal of this research was to determine the effect of methanol‐induced denitrification on volatile suspended solids production, settleability, and oxidation‐reduction potential in a full‐scale sequencing batch reactor. Batch tests were also conducted to determine the influence of mixing and acclimatization on the denitrification of wastewater with methanol. The observed sludge production in the full‐scale sequencing batch reactor with methanol addition was 0.21 kg volatile suspended solids 1 methanol, versus the calculated stoichiometric sludge production of 0.17 kg volatile suspended solids 1‐1 methanol. The settleability in the full‐scale sequencing batch reactor, measured by the sludge volume index, increases linearly with increasing denitrification rate. The total change in the oxidation‐reduction potential magnitude during a sequencing batch reactor cycle increased linearly with increasing denitrification rate. A minimum of 55% increase in the denitrification rate was observed in a batch reactor with methanol addition and a sludge acclimatized to methanol addition, compared to a batch reactor with methanol addition and a non‐acclimatized sludge. The non‐acclimatized batch reactor had a negligible denitrification rate without methanol addition. However, significant denitrification rates were observed in the acclimatized batch reactors without methanol addition, potentially caused by microbial storage or an increased population of denitrifiers that scavenge any available carbon. A completely mixed batch reactor, with sludge acclimatized to methanol addition during the anoxic cycle, had an increase in the denitrification rate ranging from 660%, without methanol addition, to 200%, with a methanol dosage of 12.7 mg 1‐1, compared to the unmixed batch reactor with an acclimatized sludge. Therefore, mixing appears to be critical to the denitrification process, to realize the best kinetic performance.