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The effects of red blood cell preparation method on in vitro markers of red blood cell aging and inflammatory response

Studies are currently under way examining whether the age of stored red blood cells (RBCs) affects clinical outcome in transfusion recipients. The effects of storage duration on the RBC storage lesion are well documented, while fewer studies are available regarding the effect of RBC production method. In this study, we compared in vitro RBC quality variables and markers of inflammatory response in apheresis and whole blood (WB)‐derived RBCs, specifically those prepared after an overnight room temperature hold (RTH) of WB.