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The Efect of Sample Prepararion and Incubation Sequence on Dry Matter Disappearance of Fresh Grass In Situ

Predictive models for estimating the nutrient requirements of ruminant livestock have become increasingly complex in their approach, and implicitly they have a need for accurate characterisation of feed degradability kinetics (AFRC, 1992; Russell et al., 1992; Sniffen et al., 1992; Fox et al., 1992). The rumen in situ technique has been adopted as the standard method (AFRC, 1992) for the characterisation of nitrogen degradability in feeds for ruminants. Recent ring tests (Madsen and Hvelplund. 1994) and reviews (Huntington and Givens. in press) have highlighted the need for improved standardisation of the procedure. The aim of this experiment was to determine the effect of sample preparation and incubation sequence on dry matter (DM) losses of fresh grass from polyester bags.