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Human breast cancer cell growth inhibition and deregulation of [Ca2+]i by estradiol

Estradiol inhibits the growth of human breast cancer MCF- 7 cells at supramlcromolar concentrations. The mechanism of such phenomenon remains to be unravelled. Confocal laser scanning microscopic studies suggest elevation of [Ca2+ ]i preceding bleb formation and cellular Injury following acute and chronic treatment of ionomycln and estradiol at supramicromolar concentration. Phase contrast morphological study demonstrates metaphase-arrested cells and giant multinuclear cells possibly due to lack of cytokinesis caused by estradiol. There is a striking similarity between the morphological changes caused by estradiol and enforced overexpression of cyclln-dependent klnase Inhibitor P21wafl/cipl. Such similarity together with the reported key role of intracellular Ionized calcium [ca2], in regulating cyclln and deregulation of [Ca2+]i by estradiol raises the possibility of deregulation of gene expression leading to Inhibition of cyclln-dependent proliferative signals participating In Inhibition of growth In MCF-7 cells.