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Production of tumor necrosis factor alpha by resident peritoneal cells of mice.

Freshly isolated resident peritoneal cells (RPC) of three mouse strains: BALB/c, NZB and C3H, release spontaneously small amounts of tumor necrosis factor (TNF) when cultured in vitro. RPC of BALB/c mice produced higher TNF levels than the cells isolated from two other strains (BALB/c > NZB > C3H). Lipopolysaccharide (LPS) potentiated the TNF production. Kinetics of the TNF production depended on temperature of incubation of the cells. At 37 degrees C TNF was produced earlier (with a peak at 7 h) than at 26 degrees C (a peak at 20 h). Adherent cell fraction of RPC produced TNF at 26 degrees C as well as at 37 degrees C. Nonadherent cells released TNF only at 37 degrees C. The neutralization assay with media containing TNF and polyclonal rabbit anti-mouse TNF-alpha serum revealed that in every case, independently on the incubation temperature and cell fraction origin, only TNF-alpha was detected. Antibodies against human monoclonal or polyclonal TNF-alpha did not neutralize mouse TNF.