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Phenotypical and Genotypical Properties of an Arcanobacterium pluranimalium Strain Isolated from a Juvenile Giraffe (Giraffa camelopardalis reticulata)

The present study was designed to characterize phenotypically and genotypically an Arcanobacterium pluranimalium strain (A. pluranimalium 4868) following necropsy from a juvenile giraffe. The species identity could be confirmed by phenotypical investigations and by MALDI-TOF MS analysis, by sequencing the 16S rDNA, pluranimaliumlysin encoding gene pla, and glyceraldehyde-3-phosphate dehydrogenase encoding gene gap with sequence similarities to A. pluranimalium reference strain DSM 13483T of 99.2%, 89.9%, and 99.1%, respectively. To our knowledge, the present study is the first phenotypic and genotypic characterization of an A. pluranimalium strain isolated from a giraffe.

The original species characterization of A. pluranimalium was performed with two strains isolated from a dead harbour porpoise and a dead fallow deer [5]. In the following years A. pluranimalium could also be isolated from a dog with pyoderma [6], from ovine specimens on 33 occasions, and from a milk sample of a single cow with mastitis [7]. More recently several A. pluranimalium strains recovered from various specimens were identified phenotypically and by using various molecular targets [8].

Material and Methods
The present study was focused on the characterization of an A. pluranimalium strain following necropsy from a juvenile giraffe by various phenotypic properties, by MALDI-TOF MS analysis, and genotypically by sequencing 16S rDNA and the A. pluranimalium-specific target genes pla and gap.
The 80.5 kg female giraffe (Giraffa camelopardalis reticulata) of the present study was born in 2013. The giraffe was not accepted by its mother or wet nurse and did not accept hand rearing attempts and, because of general weakness, was euthanized three days after birth. The subsequent postmortem analysis revealed an acute hyperemia of lung and liver and a focal emphysema of the lung. The acute pneumonia was caused by a bacterial infection associated with aspirated foreign bodies.
Bacteriological investigations yielded the isolation of A. pluranimalium and Escherichia coli, partly together with coagulase negative staphylococci, -haemolytic streptococci, and Pseudomonas fluorescens from liver, spleen, kidney, and lung. A moderate to high growth of E. coli was generally noted (++, +++); A. pluranimalium grew only in low numbers (+). The A. pluranimalium strain 4868, originally obtained from the spleen, was used for further studies. The bacterial strain was investigated phenotypically and by MALDI-TOF analysis [6,9] and genotypically by amplification and sequencing of 16S rDNA using universal oligonucleotide primer 16  For MALDI-TOF MS the isolates were prepared using the direct smear method as well as an extraction protocol provided by the manufacturer. Briefly, freshly grown bacteria were harvested and diluted in ethanol, centrifuged (2000 ×g), air-dried, and resuspended in aqueous volumes of 70% formic acid and acetonitril followed by a vortex step.

Results and Discussions
A. pluranimalium 4868 investigated in the present study was identified by determination of hemolysis and CAMPlike hemolytic reactions, by using a commercial identification system as well as various other phenotypical tests. The CAMP-like hemolytic reactions with Staphylococcus aureus -hemolysin, Rhodococcus equi, and Arcanobacterium haemolyticum as indicator strains are known as typicalcharacteristics of this species [6,8,11]. Comparable to previously investigated A. pluranimalium [6,8] the phenotypical tests also revealed the typical biochemical properties of this species (Table 1). It was of interest that A. pluranimalium 4868 of the present study was catalase negative. This was observed previously for A. pluranimalium of bovine origin [8].
As shown by numerous authors MALDI-TOF MS is a powerful tool for species identification of a broad spectrum of bacteria including Gram-positive and Gram-negative bacteria [12][13][14]. Comparable to the previously conducted MALDI-TOF MS analysis of bacteria of genera Arcanobacterium and Trueperella (formerly belonging to genus Arcanobacterium [9,15]), MALDI-TOF MS allowed the identification of A. pluranimalium 4868 of the present study to the species level matching to A. pluranimalium reference strain DSM 13483 T with a log score value of 2.28.
Sequencing 16S rDNA, the potentially cytolytic toxin pluranimaliumlysin encoding target gene pla and the glyceraldehyde-3-phosphate dehydrogenase encoding target gene gap revealed a sequence similarity of 99.2%, 89.9%, and 99.1% to the respective sequences of A. pluranimalium DSM 13483 T . All three sequences of A. pluranimalium 4868 were deposited in GenBank (HG794511, HG423389, and HG423390). A typical dendrogram of the sequencing results of the genes pla and gap is shown in Figures 1 and 2. Comparable to gene plo of T. pyogenes, which appeared to be a constant characteristic of all investigated T. pyogenes [16][17][18][19], pla of A. pluranimalium seems to be also constantly present in all strains of this species and could be used, as described previously [8], and in the present study for molecular identification of A. pluranimalium. More recently, Moser et al. 2013 [20] also described pla as novel target for molecular identification of this species.
Sequencing of gene gap had already been described for molecular identification of staphylococcal species [21] and more recently for identification of an A. haemolyticum strain isolated from a donkey [10]. In the present study gene gap could also be used as novel target for identification of A. pluranimalium. Further studies will give information about the constant presence and sequence similarities of both target genes pla and gap, respectively.

Conclusion
The clinical importance of A. pluranimalium of the present study, which was isolated from various organs of the giraffe together with in high number appearing E. coli, remains unclear. Since, beside aspiration pneumonia, no other pathological findings could be detected, this might represent the route of infection. However, the isolation of this bacterial species from giraffe and the hitherto described origin harbor porpoise, fallow deer, dog, sheep, and cow emphasizes the species name A. pluranimalium.