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[Construction of phage vector for Bacillus subtilis].

Recombinant phages phi 105S35 and phi 105S36, derived from phage phi 105DI:1t, remain the ability to infect and be lysogenized in the host cell. In the their genome there is a 1 kb inset fragment which occurs between the DNA fragments D and E resulted from SmaI degested DNA of phi 105DI:1t. But the orientation of this insert fragment is opposite in two recombinant phages, respectively. The cat gene on this fragment makes the host cell resistant to chloramphenaical. The unique BamHI and XbaI sites which were introduced into the genome of the recombinant phages provide inserting sites of foreign DNA fragment. DNA from these two recombinant phages can transfect protoplast of Bacillus subtilis at high frequency. Therefore, phi 105S35 and phi 105S36 are suitable as phage vectors in the molecular cloning of Bacillus subtilis.