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The relationship of acetylcholinesterase activity to optic fiber projections in the tiger salamander Ambystoma tigrinum

In the tiger salamnder the distribution of optic fibers, as revealed by the Fink‐Heimer method, is compared with the localization of acetylcholinesterase, as revealed by histochemical methods. AChE activity coincides with optic nerve axons in the optic fiber layer of the retina, in the optic nerve, in the optic tracts and in every optic projection. Reginons of optic fiber terminals show heavier activity than optic fibers of passage. Comparison with other vertebrates is also made.