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A Simple Description of Two-Dimensional NMR Spectroscopy

The concept of two-dimensional (2D) Fourier transformation in NMR was first introduced by Jeener (1) in 1971. Since that time, several hundred different 2D pulse schemes have been proposed in the literature. The selection of experiments that will be described is based on the insight they provide into the basis and generality of 2D NMR, and also on the importance for solving the most common types of problems. The experiments that will be described here are (a) 2D homonuclear shift correlation through scalar coupling (COSY), (b) 2D cross relaxation spectroscopy based on the nuclear Overhauser effect (NOESY) and (c) heteronuclear chemical shift correlation of H and x 5 N chemical shifts