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Aquatic vascular plants of South Brazil: checklist and a comparative floristic approach

Aquatic ecosystems support species diversity, and knowledge of plant communities is essential for wetland conservation programs. This study provides a checklist of aquatic vascular plants of South Brazil and establishes their floristic affinities with bordering South America phytogeographical domains. The checklist was based on 52 sources, including 35 floristic lists, 17 regional taxonomic studies, and information from an electronic database on wetlands of South Brazil. Floristic similarities with published checklists for neighbouring regions were assessed. A total of 780 species distributed in 277 genera and 85 families of vascular plants were reported for South Brazil. Families with higher species richness were Cyperaceae (128), Poaceae (102), Asteraceae (69), Plantaginaceae (21), Lentibulariaceae (20), and Onagraceae (20). The most represented genera were Eleocharis (41) and Cyperus (24). A cluster analysis revealed high similarity with Iberá (Argentinian Chaco), Pampa and Atlantic Forest, and low similarity with Pantanal, Caatinga and the Amazon Rainforest. The high number of aquatic plant families recorded relative to temperate and tropical climates suggests a zone of biogeographical overlap in Southern Brazil. The compiled data set reveals high biodiversity of wetlands of South Brazil, provides a baseline for future research, and highlights the need for regional conservation planning.


Introduction
Wetlands are estimated to comprise 20 % of the Brazilian territory (Neiff 2001;Junk et al. 2014). Hydrographic basins of Paraná, Uruguay River and southeast and south coast basins compose the hydrography of South Brazil. Paraná and Uruguay Rivers present an important network of tributaries, a critical component of particular interest regarding water source, energy, and tourism. The south coast basin includes the basin of the Guaíba river with its tributaries that end in the Patos Lagoon. This lagoon is the largest choked lagoon in the world, with an area of 10,227 km 2 (Asmus 1998).
The coastal plain of Rio Grande do Sul consists of a coastal lake system shaped during Pleistocene and Holocene sea transgression and regression phases (Asmus 1998). This region has more than 60 freshwater or brackish water lagoons, in addition to estuaries and floodplains.
Many authors have proposed different criteria for classifying wetlands to characterize particularities of each region (Zoltai et al. 1975;Cowardin et al. 1979;Brinson 1993;Pressey & Adam 1995). Maltchik et al. (2004) proposed a hierarchical classification of wetlands for the state of Rio Grande do Sul, with emphasis on the structure of plant communities, while avoiding regional terms. Simillarly, Junk et al. (2011) classified the Amazonian inland wetlands based on the structure of plant community. Recently, several researchers from different regions of Brazil gathered to draw up a preliminary national classification of wetlands (Junk et al. 2014), highlighting the importance of wetland inventories for governements to subsidize national policy regarding management and protection of wetlands.
This study is part of a national initiative aiming to provide a Brazilian checklist for wetland ecosystems. Subsets for the north and northeast (Moura Júnior et al. 2013;2015) and, more recently, the southeast (Pivari et al. 2018) have been already been published. Thus, in this study, we addressed the following questions: (I) how many species are recorded in South Brazil? (II) How strong are the floristic links between wetlands of South Brazil (Pampa and Atlantic Forest) and other South American wetland regions such as Pantanal, Caatinga, Amazon Rainforest, and Argentinian Chaco? For this, we compiled the available floristic data of aquatic vascular plants of South Brazil wetlands, evaluated species distribution patterns within South Brazil wetlands and their floristic affinities with wetlands of neighbouring South American phytogeographical domains.

Study area
Our study area was comprised of wetland ecosystems in South Brazil, which includes the states of Paraná (PR), Santa Catarina (SC), and Rio Grande do Sul (RS). This region presents two phytogeographic domains: Atlantic Forest and Pampa. The Atlantic Forest occupies the eastern slopes and valleys of the South Brazilian plateau, from the northeast part of Rio Grande do Sul to the coastal plain, and the highland slopes of the states of Santa Catarina and Paraná (Overbeck et al. 2007). Different forest types occur in distinct climatic conditions; grasslands form isolated patches within this forested landscape (Andrade et al. 2016). The Pampa occupies the southern half of Rio Grande do Sul, extending beyond Brazilian boundaries to Uruguay and center-east Argentina, regiong that is regionally known as Río de la Plata grasslands. In this domain, the predominant vegetation is natural grassland that covers extensive areas. Forests are mostly restricted to riversides.

Aquatic plant definition
In this study, we followed the aquatic vascular plant definitions by Cook et al. (1974); plants with photosynthetically active parts permanently or for several months of the year, totally or partially submersed in freshwater, or floating in aquatic habitats. This definition takes into consideration the complexity of different environmental factors that determine species' presence, especially regarding species that extend over a wide range of habitats with different water availability.

Data source and methods
We compiled species data of published and gray literature on South Brazil 'aquatic plants' and equivalent terms, published between the 1984 and 2015. We consulted books, national and international scientific papers, and Ph.D. and master's theses containing lists of vascular plants in South Brazil. Additional information was gathered from taxonomic studies of selected plant families containing aquatic plants, and from the electronic data set of the Brazil Flora Group (BFG 2015), which is still being compiled. We filtered records of species by region, phytogeographical domain (i.e. Pampa and Atlantic Forest), and aquatic plant type.
We selected the studies in which (i) all recorded species were classified by life forms and indicated as aquatic plants by authors, or (ii) the environment was classified as wetland. In case of unreliable occurrence, the species was not considered.
Our data included 35 floristic lists and 17 taxonomic studies, totalling 52 consulted sources (as seen in List S1 in supplementary material). From these 52 sources, 34 had geographic coordinates, while the remaining sources were taxonomic studies or general floristic lists regarding a broader region (Fig. 1). We decided to present the results according to political boundaries, hoping to stimulate new research studies in areas with little or no studies within South Brazil.
We used aquatic plants checklists of other Brazilian phytogeographical domains as Caatinga (Moura Júnior et al. 2013), Pantanal (Pott & Pott 1997) and Amazon Rainforest (Moura Júnior et al. 2015) to evaluate the floristic similarity with neighbouring regions. We also included the Argentinian area "Iberá wetlands" from the Argentinian Chaco (Arbo et al. 2002) as neighbouring region. No data set or checklist of aquatic plants of the Cerrado domain was available. However, Pantanal and Cerrado belong to the same phytoecological domain, according to Pott et al. (2011) and Cunha et al. (2016).
After data compilation, we checked nomenclature and taxonomy of the South Brazilian species list, using the Missouri Botanical Gardens' Tropicos website (http://www. tropicos.org); family classification followed the APG IV systems (2016) for angiosperms, and Smith et al. (2008) for ferns. Exotic species, infraspecific taxa or non-confirmed taxonomic entities (92 taxa) were excluded from the analysis. Distribution of plant species reported for Rio Grande do Sul was confirmed with the Global Biodiversity Information Facility (GBIF http://www.gbif.org) and Species Link (http:// splink.cria.org.br/), since two phytogeographical domains (Pampa and Atlantic Forest) are found in this state.

Data analysis
A total of 1423 species of South Brazil and other regions of South America were compiled. To evaluate vegetation similarity among different sources, we used a binary matrix with presence (1) and absence (0) of species. Thereafter, we conducted an agglomerative hierarchical clustering analysis (Legendre & Legendre 2012) for results presentation and interpretation. Similar sites were clustered and aggregated into larger and larger clusters to form a single cluster containing all sites (Roleček et al. 2009). This analysis was performed using UPGMA (average linkage) and Sørensen dissimilarity, using vegan package (Oksanen et al. 2013) in R (R Development Core Team 2013). Sørensen's coefficient quantifies the dissimilarity in species composition, i.e. values close to 0 (zero) indicate little species correlation, while values close to 1 (one) indicate almost identical species composition (high similarity).

Results
The final list included 760 aquatic plant species of South Brazil (Tab. S1 in supplementary material), distributed in 281 genera and 84 families. Some species (207)  Floristic studies were unequally distributed among the states of South Brazil. Most studies were conducted in the state of Rio Grande do Sul (RS; 57 %), followed by Paraná (PR; 29 %) and Santa Catarina (SC; 14 %). The species richness followed the same pattern, with 555, 444, and 395 species respectively.
Regarding phytogeographical domains in South Brazil, 9 % of all species were restricted to the Pampa, 42 % to the Atlantic Forest, and the remaining 49 % occurred in both, with a wide range of distribution.
Regarding the frequency of occurrence, 43 % of species were recorded in only one source, 50 % were reported in 2-10 sources, and 6 % were recorded in 11-19 sources. Less than 1 % of species were recorded in 20 or more sources, such as Hydrocotyle ranunculoides, Nymphoides humboldtiana,
In the second group, the Argentinian Chaco was clustered with two other subgroups, one composed of Pampa and Atlantic Forest RS (28 % dissimilarity), and another formed by Atlantic Forest SC and Atlantic Forest PR (36 % dissimilarity). Pampa and Atlantic Forest RS presented the highest similarity.
From the total compiled 1423 species, 26 species occurred in all domains (Tab. S1 in supplementary material), while 815 (57 %) had exclusive occurrence.

Discussion
We compiled a high number of aquatic plants (760 species) in wetlands of South Brazil from literature records. The recorded number of 555 species in RS confirmed a previous estimation by Irgang & Gastal (1996) of 400 to 500 species for the coast of Rio Grande do Sul alone. According to these authors, this number is explained by the variation of aquatic environment factors, such as light, temperature, substrate, nutrient content, and water flow act as habitat filters that may influence species' distribution and abundance across the landscape (Poff 1997;Shi et al. 2010). The habitat heterogeneity hypothesis assumes a correlation between area and its number of different habitats, and also assumes that different habitats host a different array of species (Williams 1964;Drakou et al. 2009). The total number of species recorded in South Brazil was higher than in other regions in Brazil, even when comparing Atlantic Forest and Pampa individually. It is possible that the aquatic plant diversity in South Brazil is a result of the large variability of habitats in the region (e.g. lakes, wet grasslands, lagoons, floodplains, marshes).
Out of the total, 43 % of species were registered in only one source. This was due to a restricted geographical distribution of some species that were only recorded in taxonomic works, while many other recorded species were present in habitats with difficult delimitation. This occurs because a variety of plants exhibit plasticity in response to water dynamics. It is difficult to establish a limit between aquatic and terrestrial plants, and thus, to determine the thresholds of a wetland (Lavania et al. 1990;Barrett et al. 1993;Crow & Hellquist 2000).
Cyperaceae and Poaceae were the families with the highest numbers of species in the wetlands of South Brazil, similarly to checklists of other regions (Keddy 2000;Sieben et al. 2010). The high occurrence of these families is probably explained by the success in dispersion of sexual propagules (Santamaría 2002) and ubiquitous distribution of species with relevant morphological charachteristics that allow them to spread vegetatively (e.g. rhizomes, tubers and stolons; Goetghebeur 1998).
The 26 species that occurred in all phytogeographical domains, (e.g. Eichhornia crassipes, Pistia stratiotes, Hymenachne amplexicaulis and Echinochloa polystachya) are distributed worldwide and are even considered invasive in some regions (Foxcroft et al. 2013). Although a high number of species was shown to have wide distribution not restricted to a single domain (609 spp.), the number of species with exclusive occurrence was almost 30 % higher (814 spp.). This high number may reflect the limits of species distribution by both the climatic differences of tropical and temperate zones throughout the country, and the reproductive restrictions and specific environmental conditions. Although many aquatic plants grow in a broad range of climatic regions, their distribution reflects this zonation to some extent (Santamaría 2002). Differences can be observed at the species and genera level, but are more easily seen at the the family level. For instance, in a study addressing latitudinal patterns in aquatic angiosperms, Crow (1993) highlighted some families with strong tropical affinities (e.g. Hydrocharitaceae, Limnocharitaceae, Mayacaceae, Nymphaeaceae, Podostemaceae, Eriocaulaceae, Pontederiaceae, Xyridaceae), families with temperate affinities (e.g. Potamogetonaceae, Juncaginaceae, Haloragaceae and Elatinaceae), and families well represented in both latitudes (e.g. Lentibulariaceae). Furthermore, some tropical families are among the families with the highest number of species (Xyridaceae, Lentibulariaceae and Eriocaulaceae). Families with temperate affinities (like Juncaginaceae, Juncaceae and Potamogetonaceae) are represented by only a few species in Brazil; however, the distribution of the clear majority is restricted to the south.
The transitional zone between tropical and temperate climates in South Brazil can explain the high number of families with tropical affinities; the temperate family with distribution restricted to the south region of Brazil, and the high species richness. From an evolutionary point of view, transition zones deserve special attention because they represent areas of intense biotic interaction (Morrone 2009). Its importance highlights the need for conservation approaches in these regions.
The cluster analysis based on wetland species revealed distinct distribution patterns among domains. Group 1 had low similarity, and was composed of Amazon Rainforest, Pantanal, and Caatinga. This cluster's composition is in agreement, in part, with previous studies wherein the north and northeast regions were grouped together (Moura Júnior et al. 2015;Ferreira et al. 2011). However, in the same studies, Pantanal was grouped with the Paraná River floodplain. To clarify this issue, other wetlands besides the ones in Paraná River, state of Paraná were included. Consequently, this state may have been grouped with Santa Catarina, as the Atlantic Forest encompasses both states.
The low similarities suggest floristic particularities among Amazon Rainforest, Pantanal and Caatinga. The Pantanal is often called "pantanal complex" due to its plant mosaic with elements of the major nearby vegetation types: Cerrado, Chaco and Amazon Rainforest (Prance & Schaller 1982;Cunha et al. 2007). Hence, we recommend grouping the domains Pantanal and Cerrado in future analyses regarding flora. The floristic particularities associated with the Amazon Rainforest have been previously reported by Junk (1986), who demonstrated that few aquatic plants are adapted to the extreme hydrological conditions of the region and are unable to disperse over long distances.
Group 2 included South Brazilian domains/regions with Iberá (Argentinian Chaco). This is not surprising given the high number of shared species and geographical proximity. Overall, this result is in accordance with the study of Morrone (2014) in which the Pampa domain was related with the Chaco domain, and also with the assumption of Irgang & Gastal (1996) that Uruguay, Argentina, Paraguay and South Brazil form a phytogeographic unit regarding aquatic plants.
An unexpected diversity of macrophytes of South Brazil was shown herein. The number of species will likely increase as new species are discovered, taxonomic revisions are undertaken, and new inventories are conducted in the region.