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Determination of 5‐Formyluracil via Oxime‐Based Nucleotide‐Metal Coordination

2‐(Aminooxy)‐N‐(quinolin‐8‐yl)acetamide was synthesized, and its ability to regulate activities of DNA polymerase was tested. In addition, we used the isothermal amplification technology to detect the content of 5‐formyluracil sites in irradiated genomic DNA, which confirmed its capability for the detection of 5‐formyluracil content in general samples. This study presents the first example of the determination of 5fU based on coordination chemistry.