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Therapeutic strategies and enhanced production of stem cell-derived exosomes for tissue regeneration.

Exosomes are nanovesicles surrounded by a plasma membrane and carry bioactive molecules (e.g., proteins, lipids, and nucleic acids) of the origin cell type. The bioactive molecules delivered by exosomes to the recipient cells have attracted considerable attention, as they play an important role in intercellular communication. Moreover, exosomes have unique properties, including the ability to penetrate the biological barrier with minimal immunogenicity and side effects, which can influence various physiological and pathological processes. Thus, exosomes are a promising therapeutic platform for various diseases (e.g., malignancies and allergies), as well as for the regeneration of damaged tissues. However, challenges of obtaining exosomes, such as complex extraction procedures, low yield, and difficulty in quantification are yet to be overcome, which limits the use of exosomes in clinical settings. In this review, we describe the state-of-the-art engineering techniques and strategies for highly efficient mass production of exosomes. Moreover, we discuss the functional aspects and potential therapeutic applications of stem cell-derived exosomes, and deliberate upon various engineering techniques and platform combinations for improved tissue regeneration by exosomes.