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Genome-Wide Analysis of NAC Proteins and Characterization of the ATAF Member RcNAC091 in Rose (Rosa Chinensis)


 The NAC (NAM, ATAF, and CUC) transcription factors (TFs), as plant-specific transcription regulators, play a critical role in various developmental processes and stress responses. However, knowledge of the NAC TFs in rose (Rosa chinensis), one of the most important horticultural crops, is limited. In this study, 116 NAC genes were identified from the rose genome and classified into 16 subfamilies. The RcNACs were unevenly distributed on the chromosomes. Analysis of the stress-related RcNACs based on the transcriptome data showed differences in the expression patterns in different organs and flower developmental stages and during drought and salt stress in rose leaves and roots. Analysis of the cis-regulatory elements (CREs) in the promoter region revealed that stress-related RcNACs possessed three sets of CREs, corresponding to plant growth and development, plant hormonal response, and abiotic and biotic stresses. Reverse transcription-quantitative real-time PCR (RT-qPCR) analysis of 11 representative RcNACs revealed their diverse transcript expression in leaves and roots under abscisic acid (ABA), polyethylene glycol (PEG), and sodium chloride (NaCl) treatments. Furthermore, RcNAC091 silencing verified its role in positively regulating the dehydration stress response. Overall, this study provides helpful insight into stress-related RcNACs and paves the way for stress tolerance via breeding in rose.