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Cetuximab improves AZD6244 antitumor activity in colorectal cancer HT29 cells in vitro and in nude mice by attenuating HER3/Akt pathway activation

The present study investigated the molecular mechanism by which the epidermal growth factor receptor (EGFR) inhibitor cetuximab enhances the antitumor activity of the mitogen-activated protein kinase kinase (MEK) inhibitor AZD6244 in colorectal cancer HT29 cells. HT29 cells were treated with AZD6244 plus cetuximab and then subjected to the following assays: Cell Counting kit-8, BrdU-incorporation, flow cytometric cell cycle distribution and apoptosis analysis, western blot analysis, and nude mouse xenografts. The combination of AZD6244 and cetuximab significantly reduced HT29 cell viability and proliferation compared with AZD6244 alone. The combination treatment reduced the IC50 value from 108.12±10.05 to 28.45±1.92 nM. AZD6244 and cetuximab also induced cell cycle arrest at G1 phase and reduced S phase (88.53% vs. 93.39%, P=0.080; 8.73% vs. 4.24%, P=0.082, respectively). Combination of AZD6244 with cetuximab significantly induced tumor cells apoptosis (14.61% vs. 8.99%, P=0.046). Inhibition of EGFR activity using cetuximab partially abrogated the feedback-activation of phosphorylated receptor tyrosine-protein kinase erB-3 (p-HER3) and p-AKT serine/threonine kinase (AKT), as well as prevented reactivation of p-extracellular regulated kinase (ERK) conferred by AZD6244 treatment. Combination of AZD6244 and cetuximab also inhibited HT29 cell xenograft growth in nude mice and suppressed HER3 and p-AKT levels in xenografts. The EGFR inhibitor cetuximab enhanced the antitumor activity of the MEK inhibitor AZD6244 in colorectal cells in vitro and in vivo. Co-inhibition of MEK and EGFR may be a promising treatment strategy in colorectal cancers.


Introduction
Colorectal cancer (CRC) is a significant health problem worldwide for men and women, and accounted for an estimated 1.4 million new cases and 693,900 cancer-associated mortalities globally in 2012, the global burden of the CRC is expected to increase by 60% to >2.2 million new cases and 1.1 million mortalities by 2030 (1,2). Unhealthy diets, obesity and tobacco smoking are major risk factors for the incidence of CRC, particularly in several Asian and Eastern European countries (2). Despite the implementation of active cancer screening for early detection, prevention and advanced treatment strategies in the last decade, CRC-associated morbidity remains a clinical burden (1,3,4). Thus, identifying effective treatment regimens may aid in controlling or treating the advanced stages of the disease, and improve the 5-year overall survival rate of patients.
CRC, like most other human cancer types, is defined by an abnormal growth of cells that have the ability to invade or spread to other parts of the body (5). Molecularly, gene expression-based subtyping of CRC has identified four subtypes, named consensus molecular subtypes (CMSs) that are specifically associated with different characteristic mutations. For instance, mutations of proto-oncogene B-Raf (BRAF) have been frequently observed in the CMS1 subtype and KRAS proto-oncogene GTPase (KRAS) mutations are overrepresented in the CMS3 subtype (6). Knowledge of genetic or epigenetic events in CRC has identified certain biomarkers that may be utilized to predict behavior and prognosis beyond staging, and inform treatment approaches (6,7). For example, patients with CRC with BRAF V600E mutations often have worse overall survival rates (8,9), and BRAF mutations may predict a lack of response to epidermal growth factor receptor (EGFR) monoclonal antibodies (9), including cetuximab. Furthermore, mitogen activated protein kinase kinase (MEK), is the downstream effector of RAF, and contains two homologous subtypes, MEK1 and MEK2, which are currently the only known activators of extracellular regulated kinase (ERK). Furthermore, MEK protein contains a pocket structure that closes the ATP binding site, making it an ideal target for small molecular inhibitors and a therapeutic strategy in cancer therapy, particularly for those with abnormal activation of the MAPK signaling pathway due to

Cetuximab improves AZD6244 antitumor activity in colorectal cancer HT29 cells in vitro and in nude mice by attenuating HER3/Akt pathway activation
KRAS or BRAF mutations. However, a previous study revealed poor results for a MEK inhibitor, trametinib, in patients with BRAF-mutated CRC, whereby the overall response rate was ~5% (10). This result may have been due to the crosstalk activation of receptor tyrosine kinases (RTKs). Indeed, while a single agent targets a single molecular target, cancer cells are often activated via alternate pathways as an escape mechanism to overcome such inhibition, and therefore become resistant to drugs. Lin et al (11) reported that ligand-mediated reactivation of EGFR was involved in the sensitivity reduction in BRAF-mutated non-small cell lung cancer in response to MEK inhibition (11). In addition, Mirzoeva et al (12) reported that MEK inhibitor-treated breast-cancer cells exhibited a strong feedback-activation of EGFR. In summary, release of the MEK-EGFR negative feedback loop may be one of the reasons for decreased sensitivity to MEK inhibitors in cancer, combinatorial drugs that target EGFR and MEK may be a strategy for overcoming MEK inhibitors resistance. In the present study, a therapeutic strategy to treat a subgroup of CRC cases was investigated. The growth inhibitory effect of a MEK inhibitor, AZD6244, in combination with an EGFR inhibitor, cetuximab, on the regulation of human HT29 CRC cells that harbor the BRAF V600E mutation was assessed in vitro and in an in vivo cell xenograft assay. These findings provide insight regarding preliminary molecular sensitizing mechanisms of cetuximab and AZD6244 for future clinical application in CRC treatment.

Materials and methods
Cell culture and treatment. The human HT29 CRC cell line (BRAF V600E mutation) was obtained from American Type Culture Collection (Manassas, VA, USA). Cells were maintained in RPMI-1640 (Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Beit Haemek, Israel), 100 µg/ml penicillin and 100 mg/ml streptomycin in a humidified incubator with 5% CO 2 at 37˚C.
The selective MEK inhibitor AZD6244 was purchased from Selleck Chemicals (cat. no. S1008; Houston, TX, USA) and dissolved in DMSO at 10 mM, then stored at -20˚C under light-protected conditions. The EGFR monoclonal antibody cetuximab was supplied by Daping Hospital Cancer Center and Research Institute of Surgery, The Third Military Medical University (Chongqing, China) and used at the indicated doses.
Cell viability Cell Counting kit (CCK)8 assay. HT29 cells in the exponential growth phase were seeded into 96-well plates at a density of 3x10 3 cells/well, incubated for 24 h and then treated with increasing concentrations of AZD6244 (up to 10 4 nM) with/without cetuximab (10 mg/l) for 72 h. Cell viability was assessed by adding 10 µl CCK-8 (Bio-Rad Laboratories, Inc., Hercules, CA, USA) into the cell culture and incubating for an additional 2 h. The cell culture medium was then replaced with 200 µl DMSO, prior to measurements using a spectrophotometer at 450 nM. The cells were treated in triplicate and the experiments were repeated at least three times. The 50% inhibitory concentration (IC 50 ) values were then calculated using the median-effect equation derived by Chou (13).
Flow cytometry analysis for cell cycle and apoptosis. Cells were seeded at a density of 1x10 5 cells/60 mm well, grown overnight and then treated with vehicle, AZD6244 (120 nM), cetuximab (10 mg/l) or AZD6244 (120 nM) plus cetuximab (10 mg/l) for 24 h. For cell cycle distribution analysis, cells were washed with PBS twice, fixed in 70% ice-cold ethanol overnight at 4˚C and then digested with 100 µg/ml of RNase A at 37˚C for 30 min. Next, 50 µg/ml of propidium iodide was added to the cell mixture and incubated in the dark for 30 min at room temperature. For apoptosis analysis, cells were collected and incubated with Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide following the Annexin V-FITC Apoptosis Detection kit protocol (R&D Systems, Inc., Minneapolis, MN, USA). The fluorescence intensity of each cell sample was measured using the MoFlo™ XDP flow cytometer (Beckman Coulter, Inc., Brea, CA, USA) and analyzed with KALUZA software v.1.3 (Beckman Coulter, Inc.).
BrdU-labeling assay. HT29 cells in the exponential growth phase were seeded onto coverslips in 6-well plates at a density of 1x10 5 cells/well and grown overnight prior to treatment with vehicle, AZD6244 (120 nM), cetuximab (10 mg/l) or AZD6244 (120 nM) plus cetuximab (10 mg/l) for 24 h. The cells were incubated with RPMI-1640 with FBS 20 µM BrdU for 3 h at 37˚C. At the end of the treatment period, the coverslips were washed with ice-cold PBS and fixed in 70% ethanol for 10 min at room temperature. For immunocytochemical staining, the cells were treated with 0.4% Triton X-100 containing 2 M HCl in PBS for 45 min and subsequently incubated with the anti-BrdU rat antibody (cat. no. ab6326; Abcam, Cambridge, UK) at a dilution of 1:200 in PBS containing 1% bovine serum albumin (BSA; GE Healthcare Life Sciences, Little Chalfont, UK; cat. no. CPK1075) overnight in a humidified box at 4˚C. The following day, cells were washed in PBS thrice and further incubated with an FITC-conjugated mouse monoclonal antibody against Rat IgG2a heavy chain (cat. no. 99665; Abcam) at a dilution of 1:400 at room temperature for 1 h. Following washing three times with PBS each 5 min at room temperature, slides were sealed with 20 µl fluorescence sealing solution containing DAPI (Vectashield mounting medium; Vector Laboratories, Inc., Burlingame, CA, USA). Fluorescent images were acquired using a fluorescence microscope at magnification, x100 and FITC-labeled cells were counted in at least five fields of each coverslip.
Nude mouse xenograft assay. The animal protocol was approved by the Institutional Animal Care and Use Committee of The Third Military Medical University (Chongqing, China). A total of 24 four-week old female nu/nu nude mice were purchased from Beijing Huafu Kang Biological Technology Co. Inc., (Beijing, China) with an average weight 16.84 g, and maintained in a specific pathogen-free (SPF) 'barrier' facility with controlled at room temperature and 55-62% humidity, and alternating 12-h light and dark cycles. The mice received SPF mouse chow and were allowed to drink sterile water ad libitum. For the nude mouse xenograft assay, suspended HT-29 cells in sterile PBS were injected into the right flanks of nude mice at 5x10 6 cells per mouse Tumor formation was monitored by caliper measurements twice a week and tumor volume was calculated using the modified ellipsoidal formula: (length/2) x (width) 2 . When xenografts reached an average volume of 200-250 mm 3 , mice were randomly assigned to the following groups and treated accordingly for 18 days: Vehicle group (once a day, 100 µl sterile PBS consisting of 0.2% Tween-80 and 1% methycellulose by orogastric gavage; twice a week, 100 µl sterile PBS intraperitoneally), cetuximab group (once a day, 100 µl sterile PBS consisting of 0.2% Tween-80 and 1% methycellulose by orogastric gavage; cetuximab 25 mg/kg twice a week intraperitoneally), AZD6244 group (once a day, AZD6244 50 mg/kg in 0.2% Tween-80 and 1% methycellulose in sterile PBS by orogastric gavage; twice a week, 100 µl sterile PBS intraperitoneally), and a combination of cetuximab plus AZD6244 group (once a day, AZD6244 50 mg/kg in 0.2% Tween-80 and 1% methycellulose in sterile PBS by orogastric gavage; cetuximab 25 mg/kg twice a week intraperitoneally). The tumor volumes were monitored every 3 days with a caliper. The tumor volumes repeatedly measured in each mouse were normalized to the initial volume. At the end of experiments, the mice were sacrificed and tumor xenografts were collected for analysis. All animal experiment protocols were approved by the Ethical Committee at the Institute for Experimental Animals of Daping Hospital.
Immunohistochemistry. Tumors were harvested 5 days after treatment as indicated. Tumor xenografts were fixed in a 10% buffered formalin solution immediately at room temperature for 24 h following resection from the mice. The tumor blocks were dehydrated and paraffin embedded and then sectioned into 4-µm thick slices. For immunohistochemical staining, tissue sections were baked at 60˚C for 2 h, deparaffinized in xylene, and rehydrated in a descending series of ethanol (100-50%) and further washed 5 times in ddH 2 O for 1 min each at room temperature. Slices were then soaked in PBS 2 times at room temperature for 5 min each. Sections were then boiled in citrate-based antigen unmasking solution at 121˚C for 1 min and then cooled down to room temperature, washed with PBS and incubated in a 0.3% H 2 O 2 solution in ddH 2 O for 20 min at room temperature, then washed again in PBS thrice. Next, the sections were incubated in normal goat serum (Wuhan Boster Biological Technology Ltd., Wuhan, China; cat. no. AR0009) for 50 min at room temperature, followed by incubation with an anti-receptor tyrosine-protein kinase erB-3 (HER3) antibody (cat. no. 12708; Cell Signaling Technology, Inc.) at a dilution of 1:400 with SignalStain Antibody diluent or an anti-p-AKT antibody (cat. no. 4060; Cell Signaling Technology, Inc.) at a dilution of 1:50 at 4˚C overnight. The following day, the sections were washed with PBS thrice and further incubated with biotin-labeled goat anti-rabbit IgG and HRP-conjugated streptavidin, pre-diluted by the supplier at room temperature (Biotin-Streptavidin HRP Detection Systems; cat. no. SP-9001; Beijing Zhongshan Golden Bridge Biological Technology Co., Ltd., Beijing, China) for 10 min each. Positive signal was developed in the 3,3-Diaminobenzidine (DAB) substrate solution (0.05% DAB, 0.015% H 2 O 2 , PBS) followed by counterstaining at room temperature for 5 min. The stained xenograft sections were reviewed and scored under a light microscope by one of the study investigators. Only evident staining of the cytoplasmic and/or nuclei were reviewed as positive cases. The intensity of staining was classified into four grades: -, no staining; +, definite but weak staining; ++, moderate staining; and +++, strong staining.
Statistical analysis. Each experiment was repeated at least three times. All data are presented as the mean ± standard deviation. Significance was determined using one-way analysis of variance (ANOVA) with Holm-Sidak post-hoc test for multiple comparison correction. P<0.05 was considered to indicate a statistically significant difference. Statistical analyses were performed using SPSS software version 16.0 (SPSS, Inc., Chicago, IL, USA).

EGFR suppression synergizes MEK inhibitor antitumor activity in HT29 cells.
The CCK8 assay data demonstrated that the IC 50 of AZD6244 was 108.12±10.05 nM, whereas the IC 50 of AZD6244 plus cetuximab was reduced to 28.45±1.922 nM, which was approximately a five-fold reduction compared with AZD6244 alone (Fig. 1A). In addition, the BrdU-labeling assay demonstrated that combined treatment with cetuximab and AZD6244 significantly reduced the number of proliferative cells compared with AZD6244 alone (0.6±0.21% vs. 2.1±0.28%; P=7.87x10 -5 ; Fig. 1B and C). These data indicate that cetuximab enhanced the AZD6244 antitumor activity in HT29 cells in vitro.
Combination of AZD6244 and cetuximab induces cell cycle arrest and apoptosis in HT29 cells. The effects of AZD6244 and cetuximab alone, and in combination on the induction of cell cycle arrest and apoptosis was assessed in HT29 cells. It was revealed that cetuximab alone did not significantly affect the cell cycle distribution in HT29 cells compared with the control, but AZD6244 induced G 0 /G 1 phase cell cycle arrest, with a significant increase in the G1 phase (73.05±4.76% vs. 88.53±2.56%; P=0.023) and decrease in the S phase (19.63±1.28% vs. 8.73±2.48%; P=0.007) ( Fig. 2A). In addition, compared with AZD6244 alone, the combination of AZD6244 and cetuximab significantly increased the number of cells in G1 phase (88.53±2.55% vs. 93.39±1.21%; P=0.080) and decreased the number is S phase (8.73±2.48% vs. 4.24±0.88%; P=0.082) ( Fig. 2A). Furthermore, the treatment combination markedly inhibited cyclin D1 expression compared with treatment with AZD6244 alone (Fig. 2B). In addition, it was observed that AZD6244 alone significantly induced apoptosis compared with control (ANOVA F=28.72, P=1.238x10 -4 , 8.99±0.51 vs. 5.23±0.66; P=0.004). It was also demonstrated that combination of AZD6244 with cetuximab significantly induced tumor cell apoptosis compared with AZD6244 alone (14.61±2.67% vs. 8.99±0.51%; P=0.046; Fig. 2C). Consistent with this result, the expression of cleaved poly (ADP-ribose) polymerase (PARP), a hallmark of apoptosis, was also markedly increased in the combination group compared with AZD6244 alone (Fig. 2D). However, cetuximab alone was unable to induce PARP cleavage, while AZD6244 alone slightly induced PARP cleavage in HT29 cells compared with the control.

Combination of AZD6244 and cetuximab abrogates feedback-activation of ERK and AKT.
To address the molecular mechanism underlying the synergistic effects of AZD6244 and cetuximab in CRC cells, western blot analysis of MAPK and phosphatidylinositol 3-kinase (PI3K) signaling pathway-associated proteins was performed. It was revealed that p-ERK1/2 levels were transiently inhibited after treatment with AZD6244 and recovered by 24 h, and showed re-accumulation at 48 h (to ~60% of the initial level; Fig. 3A). This finding indicates that treatment with AZD6244 leads to reactivation of the MAPK signaling pathway. However, the treatment combination of AZD6244 and cetuximab markedly inhibited p-ERK1/2 and prevented re-activation (Fig. 3B). Although the combination of EGFR inhibitor cetuximab with AZD6244 significantly enhanced the HT29 cell growth inhibition rate, AZD6244 did not increase EGFR phosphorylation (Fig. 3A). However, the total protein expression and phosphorylation of HER3, another member of the HER family, were markedly upregulated following AZD6244 treatment, suggesting that HER3 is activated in response to MEK inhibition (Fig. 3A). In addition, AZD6244 activated p-AKT, an important signaling protein in the PI3K pathway (Fig. 3A). The combination of AZD6244 and cetuximab abrogated the feedback-activation loop of HER3/AKT, which was activated by AZD6244 alone (Fig. 3B).

Combination of AZD6244 and cetuximab increases treatment efficacy in CRC cell xenografts.
The effect of the treatment combination was further assessed on nude mice CRC cell xenografts in vivo. The data demonstrated that palpable tumors (200-250 mm 3 ) formed in all animals two weeks following the HT29 cell injection. The mice were then treated with vehicle alone, cetuximab (25 mg/kg), AZD6244 (50 mg/kg) or their combination at the same individual dosage (25 and 50 mg/kg, respectively) for 18 days. The data indicated that cetuximab alone had no significant effect on the growth of tumor cell xenografts, while AZD6244 alone marginally inhibited tumor growth. In contrast, the treatment combination significantly inhibited growth of HT29 cell xenografts. In addition, treatment with AZD6244 alone or in combination with cetuximab was well-tolerated by mice as no weight loss, or signs of acute or delayed toxicity were observed in the mice (Fig. 4A and B). Furthermore, the tumor volumes at the end of the experiment in the combination treatment group were notably smaller, compared with AZD6244 alone (Fig. 4C). Furthermore, immunohistochemical analysis of xenograft tissues confirmed that the treatment combination suppressed HER3 and p-AKT expression levels compared with AZD6244 alone (Fig. 5). Although the expression of total HER3 was slightly depressed, this result was consistent with the western blotting results as shown in Fig. 3A and B.

Discussion
The MAPK signaling pathway is a key downstream effector of growth factor receptors and is often dysregulated in a variety of human cancer types. For example, mutations in or overexpression of KRAS and BRAF, together with certain other growth factor receptors, including EGFR and HER2, result in increased cell growth and transformation of malignant cells (14). As MEK is upstream of the ERK and based on the pocket structure near the ATP-binding site in its molecular structure, it is an ideal target for developing small molecule inhibitors (15). In the current study, growth inhibitory effect of the MEK inhibitor AZD6244 was explored in colon cancer HT29 cells. The findings were consistent with data from previous studies (16,17). AZD6244 led to dose-dependent growth inhibition, but the p-ERK levels were only transiently suppressed in HT29 cells in vitro. A previous study revealed that re-activation of the MAPK signaling pathway contributed to the relative insensitivity of the BRAF inhibitor in CRC cells with BRAF mutations (17). The HT29 cells used in the current study also contain a BRAF mutation, and the MEK inhibitor AZD6244 was able to re-activate MAPK signaling. Furthermore, it was observed that AZD6244 treatment alone induced p-AKT levels. Thus, we hypothesize that the poor reactivity of BRAF mutant CRC cells to the MEK inhibitor AZD6244 is attributed to the re-activation of p-ERK and feedback activation of p-AKT. Although it has been well established that the presence of a BRAF mutation predicts a lack of response to monoclonal EGFR antibodies (9), including cetuximab, the current data demonstrated that the addition of cetuximab improved the antitumor activity of AZD6244 in HT29 cells that have BRAF mutations. Furthermore, the combination of AZD6244 and cetuximab suppressed p-ERK and p-AKT activation. However, the current data are just proof-of-principle and further studies with more cell lines are required to confirm these results.
Although the combination of cetuximab and AZD6244 significantly enhanced the growth inhibitory effect, and inhibited the re-activation of p-ERK and the feedback activation of p-AKT, the current study did not observe any significant increase in p-EGFR expression. However, markedly increased levels of total and phosphorylated HER3 protein expression following AZD6244 treatment in HT29 cells were noted. Functionally, p-HER3 has a high affinity for PI3K and a previous study demonstrated that HER3-mediated activation of the PI3K-AKT pathway led to resistance of non-small cell lung cancer and gastric cancer cells to MEK inhibition (18,19). However, this MAP2K-HER3-AKT negative-feedback loop requires further investigation in other types of cancer. The present study reported the MAP2K-HER3-AKT negative-feedback loop was also presented in colon cancer cells. Furthermore, combinatorial treatment with cetuximab suppressed p-HER3 and p-AKT, indicating that the attenuation of HER3/AKT feedback by cetuximab is partially responsible for the increase of AZD6244 antitumor activity. In addition, a previous structural biology study demonstrated that HER3 is a catalytically impaired member of the HER family and that its signaling is dependent on a hetero-dimerization with a catalytically active partner, in particular, EGFR or HER2 (20). A previous study revealed that EGFR and HER2 signals serve a salvage role, and are associated with resistance to MEK inhibitors in gastric cancer (21). Thus, the current study suggests that the cetuximab-abrogated feedback activation of p-HER3 may be at least partially due to blockage of EGFR/HER3 heterodimer formation. A previous study suggested that the CDC25 downregulation-mediated reactivation of EGFR led to relative insensitivity of BRAF-mutated CRC cells to a BRAF inhibitor (17). In the present study no increase in EGFR activation following AZD6244 treatment was observed; thus, the mechanism of cetuximab-mediated suppression of MAPK re-activation requires further investigation.
The current study also assessed the effects of AZD6244 in combination with cetuximab on the induction of tumor  with AZD6244 (120 nM) with or without cetuximab (10 mg) at the indicated time points, after which whole-cell extracts were blotted with antibodies against p-EGFR, total-EGFR, p-HER3, total-HER3, p-MEK1/2, total-MEK1/2, p-ERK, total-ERK, p-AKT and total-AKT. (B) HT29 cells were treated with vehicle, cetuximab (10 mg), AZD6244 (120 nM) or cetuximab (10 mg) plus AZD6244 (120 nM) for 24 h, after which whole-cell extracts were blotted with p-EGFR, total-EGFR, p-HER3, total-HER3, p-MEK1/2, total-MEK1/2, p-ERK, total-ERK, p-AKT and total-AKT. Cetu, cetuximab; p, phosphorylated; t, total; AKT, AKT serine/threonine kinase; EGFR, epidermal growth factor receptor; HER3, receptor tyrosine-protein kinase erB-3; MEK, mitogen-activated protein kinase kinase; ERK, extracellular regulated kinase. cell cycle arrest and apoptosis. It was demonstrated that the combinatorial treatment further increased the number of tumor cells arrested at G1 phase and decreased the cells in S phase, accompanied by a significantly increased percentage of apoptotic cells. These data were supported by inhibition of cyclin D1 expression and induction of cleaved PARP in HT29 cells following combinatorial treatment. In addition, the nude mouse xenograft assay further confirmed the in vitro data, indicating that the combination of AZD6244 and cetuximab may have merit as a treatment strategy for human patients with CRC. However, further studies are required to confirm whether  3 in size and then the mice were treated with vehicle, cetuximab (25 mg/kg), AZD6244 (50 mg/kg) or cetuximab (25 mg/kg) plus AZD6244 (50 mg/kg) for 18 days, and tumor xenografts volumes were plotted. The tumor volumes repeatedly measured in each mouse were normalized to the initial volume. Data are presented as the mean relative fold changes ± standard error. ** P<0.01 compared with AZD6244 alone. (B) Following treatment with the drugs, mouse weight was measured twice a week using an electronic balance. Data are presented as the mean mouse weights ± standard error. (C) Xenografts after 18 days of treatment. Selected xenograft gross images of representative cases are presented after sacrifice of the mice. Cetu, cetuximab. Figure 5. Effects of AZD6244 and cetuximab combination on regulation of p-AKT and t-HER3 expression in xenografts. HT29 cell xenografts were resected from the mice after 5 days of treatment with indicated agents and processed for immunohistochemical analysis of p-AKT and t-HER3 expression (magnification, x200). Cetu, cetuximab; p, phosphorylated; t, total; AKT, AKT serine/threonine kinase; HER3, receptor tyrosine-protein kinase erB-3.
the combinatorial treatment only works in BRAF-mutated CRC cells.
Due to the crosstalk between the RTK-signaling pathways, no signal gene dependency is to be expected. Numerous studies have reported that when cancer cells are treated with drugs that block single molecular target, they are often able to relief of feedback and activate alternate pathways as escape mechanisms to overcome the blockade. Combined inhibition of specific pathways induced by negative feedback should be an effective strategy to enhance antitumor activity (22)(23)(24). Chandarlapaty et al (22) reported that AKT inhibitor relieves the feedback suppression of RTK, combined inhibition of AKT and HER kinase activity may improve anticancer activity. In this current study, it was determined that the feedback activity of HER3/AKT contributes to the HT29 cells relative insensitivity to MEK inhibitor AZD6244, combination of AZD6244 and cetuximab can abrogate the HER3/AKT feedback activation and improve the antitumor activity of AZD6244. These findings suggest that co-inhibition of MEK and EGFR may be a promising treatment strategy in CRCs, at least those that possess a BRAF mutation. Although this work suggests that HER3-mediated feedback re-activation of p-AKT partially contributes to HT29 cell insensitivity to AZD6244, we hypothesize that additional growth factors may contribute to this treatment resistance. In our future studies, the mechanism of combinatorial treatment will be further explored.