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[Matrix metalloproteinase-9 expression by endothelial cells incubated with peripheral blood leukocytes from patients with cerebral infarction].

OBJECTIVE
Matrix metalloproteinase-9 (MMP-9) have been implicatedin the development of cerebral infarction. In a prospective study we investigated whether the MMP-9 expression are increased by endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions.


METHODS
Using Immuno-fluorescence technique, Human umbilical vein endothelial cells were cocultured with leucocytes for 24 hours in vitro before used for detecting the MMP-9 expression. The leucocytes were purified from peripheral blood in 6 patients with acute cerebral infarction and in 6 age- and sex-matched healthy subjects. the smears of endothelial cells were stained with MMP-9 monoclonal antibodies, and detected using the ABC ELITE kit.


RESULTS
Laser-Confocus confirmed the presence of MMP-9 within endothelial cells incubated with peripheral blood Leukocytes from patients with acute cerebral infarctions. Compared with the control group, MMP-9 expressions were significantly higher in the stroke group (P < 0.001).


CONCLUSION
Our study demonstrated that the leukocytes from acute cerebral infarction patients were Activated, and could stimulate vessel endothelial cells excrete MMP-9. Activated leukocytes and MMP-9 expression by endothelial cells may have an important role in acute cerebrovascular ischemia and its consequences.