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[In situ characterization of immunologically competent cells during the growth of a transplanted murine sarcoma].

To characterize the immunocompetent cell populations which infiltrate a transplantable MCA-induced sarcoma and to study their modifications during tumoral growth, we used fluorescent antibodies to the cell membrane antigens Lyt-1, Lyt-2 and Asialo GM1. Early after tumor graft, an accumulation of cells bearing the Asialo-GM1 antigen was observed; this population corresponds mostly to NK cells. Simultaneously, a macrophage infiltration, identified by a cytochemical method, was seen. After this period, an accumulation of cells bearing the Lyt-1 antigen was observed; Lyt-2 positive cells were detected continuously during experimental period.