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Small-angle light scattering in the study of phase separation in eye lens homogenate

The homogenate of the nuclear part of the bovine lens was investigated. The angular dependence of the light scattered intensities was measured as a function of temperature. The random density fluctuation theory and the Debye theory for a spherical scatterer were adopted to evaluate size of scattering units. The analysis of the experimental data demonstrates the presence of four kinds of scattering units: aggregates, separation between aggregates, scatterers responsible to the phase separation and large elements. The temperature dependence of the light scattered intensity was used to estimation of the spinodal temperature for the sample.