{'fraction_non_alphanumeric': 0.03183673469387755, 'fraction_numerical': 0.03183673469387755, 'mean_word_length': 5.4867724867724865, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Cloning of Xa 21-Like Protein Kinase Genes and Its Sequence Comparison at the Orthologous Loci in Triticum

Three Xa 21-like protein kinase genes were cloned from Triticum,and the sequences at the orthologous loci were studied.Near the HMW-glutenin gene loci of wheat(Triticum aestivum),there is a locus(temporarily named TaXa) encoding an LRR-receptor like protein kinase,which is homologous to rice (Oryza sativa) disease resistance protein Xa 21.Through RT-PCR approach,three cDNA clones of ZS 860(GenBank accession No.EF 394367),ZS 2000(GenBank accession No.EF 394368) and ZS 2001(GenBank accession No.EF 394369) were isolated at the orthologous loci of TaXa in T.aestivum and Triticum turgidum respectively.The ancestral TaXa locus probably encoded a 1 028 amino acid long peptide.A complete TaXa protein includes a N-terminal conserved sequence, LRR domains,a transmembrane region and a serine/threonine protein kinase domain.Nucleotide base pair substitution,deletion and insertion happened during gene evolution led to the change of the open reading frame,and thus shortened the encoded peptides.The amino acid sequences encoded by TaXa orthologous loci in Triticum and one of Hordeum vulgare were compared in detail.