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Ribosome fractions from normal and methylcholanthrene-treated mouse epidermis.

Ribosome fractions were isolated from normal, trypsinized epidermis of CD-1 female mouse skin. About 83%, of the ribosomes are free, and approximately 17% are membrane-bound. Almost all the ribosomes are found in the post-mitochondrial supernatant fraction. Epidermis 4 days after treatment with 3-methylcholanthrene shows a marked proportionate increase in both free and membrane-bound ribosomes. Again almost all the ribosomes are in the post-mitochondrial supernatant fraction.