{'fraction_non_alphanumeric': 0.035147392290249435, 'fraction_numerical': 0.013605442176870748, 'mean_word_length': 4.886666666666667, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Nitric oxide synthase expression in human neuroblastoma cell line induced by cytokines

The human neuroblastoma cell line NB-39-nu expressed mRNA coding for inducible nitric oxide synthase (iNOS) following treatment with a combination of interferon-7 (IFN-γ) and tumour necrosis factor-α (TNF-α). The level of iNOS mRNA peaked 24 h after stimulation and had declined by about 25% after 48 h. Trace levels of iNOS mRNA were detected after treatment with IFN-7 alone, and its mRNA level was synergistically enhanced by simultaneous treatment with TNF-α. Neither bacterial lipopolysaccharide nor interleukin-1β (IL-1β) showed synergistic effects as great as that of TNF-α on iNOS gene expression. Dexamethasone inhibited the induction of iNOS mRNA by IFN-γ and TNF-α. Induction of iNOS was confirmed by NADPH-diaphorase staining and by immunostaining with human iNOS-specific antibody.