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[DNA extraction and sex determination for human teeth dated 3000 years ago unearthed in Xi'an].

OBJECTIVE
To extracted DNA from ancient human teeth dated 3000 years ago unearthed in Xi'an and determine the genders for the individuals.


METHODS
Thirty five ancient human teeth were studied. A 'Reverse-root-canal' technique and a Chelex-100 solution were used to extract the DNA. Specific primers for Amelogenin gene were designed for PCR amplification.


RESULTS
Genomic DNA was successfully extracted from 30 samples, for which 8 were determined to be males and 22 were females.


CONCLUSION
The 'Reverse-root-canal' technique may be used for extracting DNA from ancient human teeth. Genetics method can supplement physical anthropology for determination of sex for ancient samples.