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Plasma testosterone: a simple assay without chromatography for the clinical laboratory.

A simple but accurate and specific method for the assay of plasma testosterone in the plasma of male and female subjects is described. No form of chromatography is required. The extraction and purification of the plasma testosterone depend on a series of rapid solvent partitions and utilize a standard competitive protein-binding technic. Twenty plasma samples can be conveniently processed in 6 hours by one technician. One half ml. male plasma or 1.0 ml. female plasma is required. Recovery of testosterone added to plasma and taken through the method was 72 ± 3.7 (S.D.) per cent. The reproducibility of the assay on pooled male plasma was 5.2% and on pooled female plasma, 13.1%. Results obtained by this procedure and a double-isotope-derivative method were very similar. Application of the method to samples obtained from patients produced results consistent with clinical findings.