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Fourier Transform Infrared (FTIR) Of Aqueous Solutions Of Proteins

Aqueous solutions of the major blood proteins were studied using FT-IR in transmission and ATR mode. Studies concerning the spectral effects of concentration, pH, time and flow rate on individual proteins and mixtures of model proteins will be discussed. Conformational and structural changes demonstrated in the transmission spectra have been related to variations seen in adsorbed species studied by ATR of flowing systems. By various data manipulation techniques such as deconvolution, spectral subtraction and derivative spectra, components of a mixture can be identified.