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[Study of human tumor tissues by Raman imaging spectra].

The Raman mapping spectra were collected from the samples of fresh human breast tumor tissues with the excitation wavelength at 633 nm, their correlation coefficients with the reference spectra of chemical DNA, cytoplasm and extra-cellular matrix etc. were calculated, and the images were created based on these correlation coefficients. Examining these images, the authors found that they show approximately the distribution of the corresponding reference chemicals in human tissues and that the DNA spectrum correlation images reflect the DNA distribution quite well because of its much lower correlation with the spectra of other morphological chemicals. This work is helpful in understanding the chemical/morphological basis of the breast tissue Raman spectra and in developing the Raman diagnostic method for breast tumor.