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Comparative analysis of peste des petits ruminants virus tropism in Vero and Vero/SLAM cells

A comparative study was conducted to investigate tropism of peste des petits ruminants (PPR) virus in Vero and Vero/SLAM (signaling lymphocyte activation molecule) cells. PPR virus was isolated from tissues and blood samples of sheep and goats following second passage in both the cell line. Intensive cytopathic effects and higher antigen load (by sandwich enzyme linked immuno sorbant assay [ELISA]) were observed in Vero/SLAM cells as compared to Vero cells. A significantly higher virus titer (i.e. ranging from 4.5 to 6.5 log10TCID50/ml) was detected in Vero/SLAM than Vero cell isolates (i.e. ranging from 3.5 to 4.5 log10TCID50/ml). The present study has demonstrated that Vero cells expressing the SLAM receptors are highly efficient for isolating PPR virus (PPRV) from pathological samples, as it offers a substantial improvement including faster viral replication resulting in primary viral isolation in a shorter period of time, and higher yield of virus than traditional cell culture methodologies.