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Habitat suitability and ecological niche profile of major malaria vectors in Cameroon

Background Suitability of environmental conditions determines a species distribution in space and time. Understanding and modelling the ecological niche of mosquito disease vectors can, therefore, be a powerful predictor of the risk of exposure to the pathogens they transmit. In Africa, five anophelines are responsible for over 95% of total malaria transmission. However, detailed knowledge of the geographic distribution and ecological requirements of these species is to date still inadequate. Methods Indoor-resting mosquitoes were sampled from 386 villages covering the full range of ecological settings available in Cameroon, Central Africa. Using a predictive species distribution modeling approach based only on presence records, habitat suitability maps were constructed for the five major malaria vectors Anopheles gambiae, Anopheles funestus, Anopheles arabiensis, Anopheles nili and Anopheles moucheti. The influence of 17 climatic, topographic, and land use variables on mosquito geographic distribution was assessed by multivariate regression and ordination techniques. Results Twenty-four anopheline species were collected, of which 17 are known to transmit malaria in Africa. Ecological Niche Factor Analysis, Habitat Suitability modeling and Canonical Correspondence Analysis revealed marked differences among the five major malaria vector species, both in terms of ecological requirements and niche breadth. Eco-geographical variables (EGVs) related to human activity had the highest impact on habitat suitability for the five major malaria vectors, with areas of low population density being of marginal or unsuitable habitat quality. Sunlight exposure, rainfall, evapo-transpiration, relative humidity, and wind speed were among the most discriminative EGVs separating "forest" from "savanna" species. Conclusions The distribution of major malaria vectors in Cameroon is strongly affected by the impact of humans on the environment, with variables related to proximity to human settings being among the best predictors of habitat suitability. The ecologically more tolerant species An. gambiae and An. funestus were recorded in a wide range of eco-climatic settings. The other three major vectors, An. arabiensis, An. moucheti, and An. nili, were more specialized. Ecological niche and species distribution modelling should help improve malaria vector control interventions by targeting places and times where the impact on vector populations and disease transmission can be optimized.


Background
The interactions between a species and its environment are reflected in the distribution of its abundance in both space and time [1]. Species are expected to be non-randomly distributed across different ecological settings, as a result of their specific ecological requirements and tolerance towards deviations from their optimal conditions [2,3]. Predictions of species geographic distributions can be based upon mathematical models relating field observations of occurrences to a set of environmental variables [4,5]. This kind of approach has been used to explore ecological niche requirements and to predict the potential distribution of a focal species [6]. Such predictions can be used to tackle a wide range of issues such as conservation of biodiversity, the management of species of economic interest, or evaluation of the risks linked with biological invasions [7][8][9][10]. Species distribution models are also gaining interest as a tool to evaluate and/or predict the risk of exposure to infectious diseases and their vectors, such as malaria [11][12][13][14], Chagas disease [15] or dengue [16]. Risk maps have been produced by correlating georeferenced epidemiological and environmental data to describe, explain and predict malaria risk at localities where epidemiological data are not available [11,17,18]. Mosquito life-history traits, such as growth rates and survival and the duration of the sporogonic cycle of Plasmodium in its vector, are strongly dependent upon temperature and moisture conditions on the ground. Thus, eco-climatic profiles inferred from remotely sensed images can be used as predictors of mosquito distribution patterns and average levels of transmission of malaria parasites by these vectors [12].
Malaria transmission dynamic is highly variable throughout Africa. These variations mirror, at least to some extent, the great heterogeneity of eco-climatic settings present across sub-Saharan Africa [19]. In this continent, about twenty out of 140 anopheline species have been incriminated in malaria transmission [20,21]. However, only five species are responsible for more than 95% of the overall transmission, and are therefore considered the major malaria vectors in Africa: Anopheles gambiae, Anopheles arabiensis, Anopheles funestus, Anopheles moucheti, and Anopheles nili [19,21]. The remaining 5% is due to "secondary" malaria vectors of local importance. Differences in ecological requirements, longevity and feeding behaviour (e.g. anthropophily) account for the different roles played by major and secondary vectors in malaria transmission [22]. Whereas variations in longevity and anthropophily within and between vectors species have been documented under a wide range of settings, qualitative and quantitative assessments of species' ecological requirements are still few, even for major vector species [19,23]. This paper focuses on the determination of ecological requirements for malaria vectors in Cameroon, a country in Central Africa covering a wide range of ecological and climatic domains. This great environmental heterogeneity increases the diversity of the malaria transmission system, with as much as 48 anophelines species reported [24][25][26], among which 17 have been found infected with human malaria parasites [22,[27][28][29][30]. Geographical Information Systems and Ecological Niche Factor Analysis (ENFA) [3] were employed to build predictive habitat suitability maps for the five major malaria vectors on a country-wide scale, and to compare their respective ecological requirements and niche parameters. In addition, the ecological habitat profiles for the 10 most common anopheline species found in Cameroon were described using Canonical Correspondence Analysis (CCA). This study improves current knowledge of malaria vector distribution in Cameroon and highlights relevant similarities and differences in ecological requirements of the different mosquito species present in this area. The relevance of these findings for malaria vector control in Africa is discussed.
Topographic map of Cameroon Figure 1 Topographic map of Cameroon. Localities sampled for the mosquito domestic fauna are shown as dark dots (N = 386) among all the recorded populated places present across Cameroon shown as gray dots (N = 10,700). Dotted lines delimit the main bio-geographic domains [31].

Environmental data
The study area was modelled as a raster map (1 km 2 per pixel) overlaid on the UTM Coordinate System (Zone 33N and 32N for Cameroon). Four classes of environmental descriptors, totalling 17 eco-geographical variables (EGVs), were used in the analysis: (i) topographic variables [source: Shuttle Radar Topography Mission-SRTM [40], including elevation (in meters), slope (derived from elevation data), aspect (derived from slope data) and hydrographic network (computed as a quantitative raster layer, attributing to each pixel its minimum distance to a water body). Each layer was formatted in a quantitative raster format. This was achieved using the Spatial Analyst extension in ArcGIS v8.3 with a number of customized scripts and downloads for geoprocessing available from the ESRI websites [41,42] and the software Erdas Imagine 8.0 for resampling of corrected/updated data at the appropriate resolution (i.e., 1 km 2 cells).
(ii) climatic variables [source: LocClim database developed by the Food Agriculture Organization -FAO [43], including rainfall (in mm), temperature (in °C), evapotranspiration (in mm), relative humidity (water vapour pressure in %), mean number of hours of sunlight per day (hours), and wind speed (in m.s -1 ). These data were yearly means, averaged over the past 30 years. The data from individual field weather stations were interpolated using an inverse distance weighting algorithm in the Spatial Analyst module of ArcGIS v. 8.3; (iii) habitat variables [source: Global Land Cover 2000 Project [44]. This database embodied 22 land cover types, however due to computational constraints, the number of variables entered in the analysis was reduced to 5 classes of land cover: dense evergreen forest, deciduous woodland, forest/savannas mosaic, dry savannas and cropland. Each land cover type constituted a separate layer that was encoded in Boolean format; (iv) variables related to human population pressure on the environment [Geonet Name Server [45], updated for Cameroon by the Dyepca Project developed by the US140 of IRD in Montpellier, France] including minimum distance to localities and minimum distance to the main road network.
The distribution of each EGV was normalized by the Box-Cox algorithm [46]. All layers were smoothed using the median index calculated over the 10 × 10 neighbouring spatial units of a focal cell [3,47] and exported as Idrisi files [48] into the software Biomapper 4.0 [49] to perform an Ecological Niche Factor Analysis (ENFA).

Ecological niche factor analysis
The ENFA is a species distribution model based on the ecological niche concept developed by Hutchinson [2] in a multivariate statistical framework. This model has been described in detail by [3,4] and [50]. The ENFA uses only data on the presence of the target species, and requires a raster map describing species occurrence, encoded in Boolean format. Villages where the focal species was observed were given the value of 1, while villages where the species was not found were encoded as 0; the latter are not taken into account by the ENFA. The ENFA summarizes the overall information by two indices. The first is termed 'marginality': it maximizes the multivariate distance of EGVs between the cells occupied by the species and the cells within the whole reference area (e.g. the whole of Cameroon). This index provides information about the extent to which the species niche optimum departs from the most frequent set of eco-geographical conditions in the spatial multivariate reference set. Global marginality close to one means that the species lives in a particular habitat relative to the reference area. Conversely, a value of zero indicates that the species is found everywhere [3]. The second and subsequent factors are termed 'specialization' factors: they account for the decreasing residual variance after removal of upperranked explanatory factors, and denote the extent of the species EGVs distribution width with respect to the overall distribution of the EGVs in the whole reference area. The inverse of specialization is therefore a measure of the species tolerance to conditions that are increasingly different from its optimum [3,9]. A global tolerance of one indicates no specialization at all; any value below one indicates some form of specialization. Marginality and specialization are uncorrelated factors, with the bulk of the information contained within the first factors [3].

Habitat suitability maps
Habitat suitability (HS) maps represent the variation in space of the likelihood of occurrence of a focal species. HS maps are based on the calculation of a Habitat Suitability Index (HSI). The HSI was calculated using the median algorithm based on the first significant factors obtained by the ENFA [3,4,50]. The number of significant factors included in the analysis results form the comparison of each factors' eigenvalues to a MacArthur's broken-stick distribution [3,9]. On one factor axis, the HSI calculation is based on a count of cells from the species distribution that lay at least as far apart from the median as the focal cell. This procedure is repeated for each factor included in the HSI calculation. The HSI varies from 0 (worst habitat) to 100 (best habitat), and indicates how the environmental conditions of a spatial unit expressed as a linear combination of the EGVs suit the requirements of the focal species. However, as noted by Hirzel [4], maps produced through the use of a continuous HSI are often spurious and misleading. Reclassified maps using only a few classes of HS are likely to be more accurate in regards to their actual informative content [9]. The number and boundaries of HS classes were defined following Hirzel et al, through a detailed inspection of the predicted-to-expected frequency curves (P/E, Boyce's area-adjusted frequencies) obtained for each mosquito species, as implemented in Biomapper 4.0 [49]. Four classes of HS were defined: unsuitable, marginal, suitable and optimal habitat, according to the best bin combinations of the Boyce Index B4 (see below). The boundaries between each class were set as follows: habitat suitability with no presence points (P/E = 0) denotes unsuitable habitat; habitat suitability values for which presences are less frequent than expected by chance alone (0<P/E<1) define marginal habitat; suitable and optimal habitat shared habitat suitability values for which presences are more frequent than expected by chance (P/E>1), the boundary being placed so as to maximize the P/E difference between them and limit overlap in P/E values.
The HS model's predictive power and accuracy was evaluated by means of a Jackknifed 10-fold cross-validation procedure [51] available in Biomapper 4.0 [49]. Briefly, the species locations are randomly partitioned into k = 10 mutually exclusive sets. k-1 partitions are be used to compute a HS model and the remaining partition is used to validate it on independent data. This process is repeated k times, each time by leaving out a different partition. This results in k more-or-less different HS maps. By comparing these maps and how they fluctuate, one can assess their predictive power. By default in Biomapper 4.0, the partitions are set so that they do not overlap geographically. This makes the cross-validation more robust to spatial auto-correlation (e.g. conspecific attraction). Three model evaluation indices were calculated for each replicate and were characterized by their mean and standard deviation across replicates. The Absolute Validation Index (0 = AVI = 1) is the proportion of presence points in the evaluation partition falling in spatial units with a HSI>50. The AVI indicates how well the model discriminates high-suitability from low-suitability areas [9,47,52]. The Contrast Validation Index (0 = CVI = AVI) is the difference between the AVI of a replicate and the AVI of a null model that would predict habitat suitability at random. The CVI indicates how much the model differs from a random model of habitat suitability. These two measures determine how good the model is at discriminating between suitable and unsuitable habitats for the focal species, but they depend on the choice of an arbitrary threshold (in this case, HSI = 50). The threshold-independent Boyce indices [4,53] were used to provide a more continuous and more reliable measure of the accuracy of the model's predictions. The continuous Boyce index, calculated through the use of a 'sliding window' across the range of HSI values, measures the monotonic increase of the P/E frequency ratio with increasing habitat suitability and is computed by the Spearman's rank correlation coefficient between P/E and HSI. This index varies from -1 to 1, with 0 indicating a random model [4,53]. Similarly, the sequential Boyce index B4 was used to assess the predictive power and robustness of the reclassified maps (cf. above), and was computed as the Spearman's rank correlation coefficient between P/E and mean HSI value for each of the four HSI classes defined above, averaged over 10-fold resampling.

Ecological niche differentiation across species
In order to compare the ecological requirements of different mosquito species in Cameroon, discriminant analyses were performed between pairs of species' ecological niches. While the ENFA compared the distribution of EGVs where a focal species was present to that of the whole reference area, discriminant analysis compared the distribution of EGVs of two species, with the aim to compute a factor that maximizes the difference between species while minimizing the intra-species variance. As this factor is a linear combination of the EGVs, its coefficients can be interpreted to identify which EGVs contributed the most to discriminate the ecological niche of the two species. Hence, the discriminant function indicates for which variables the two species differ most. These computations are integrated in a module of Biomapper 4.0 49 [49] and were carried out for each combination of species' pairs among the five major malaria vectors.

Canonical correspondence analysis
CCA was carried out to explore the relationship between mosquito species and EGVs using CANOCO v4.0 [54]. CCA assesses the main pattern of variation in community composition accounted for by the environmental variables and draws a graphical representation of the contribution of each EGV to the global species distribution. This analytical technique allows a quick appraisal of how community composition varies with the environment. As all mosquitoes were collected in human settlements, variables related to the human influence on the environment could bias the analysis. Moreover, distance to water bodies was not informative when the ENFA was performed (see Results). Thus, distances to roads, localities and to water bodies were removed from the set of EGVs fitted by CCA. Species data were encoded as presence (1) or absence (0) for each of the sampled localities. Both values were taken into account in the posterior analysis. Statistical significance of the canonical axes was tested with a Monte Carlo permutation test using 5000 permutations.

Anopheline species diversity and distribution
Twenty-four anopheline mosquito species were recorded during the surveys (Table 1, Figure 2 and see Additional file 1); of these, 17 have been reported as primary or secondary malaria vectors in Africa [22,27,30,55,56]. Some species, among which are all the major malaria vectors, belong to groups or complexes of morphologically similar or identical species [19,34] were recorded from a single village each. Only An. gambiae and An. funestus were widely distributed across all bioclimatic domains throughout the country, whereas most of the other species occurred in a specific domain. For example, An. arabiensis was predominant in the northernmost and most arid regions, while An. moucheti and An. nili were found mainly in the humid forest areas in the South. The greatest species richness was observed in the rainforest, with 18 out of 24 species present.

ENFA
The accuracy of the ENFA and HS maps depends upon the number and distribution of presence points in the data set [3,50]. It was possible to compute the ENFA only for the five most common malaria vectors in Cameroon: An. gambiae, An. funestus, An. arabiensis, An. moucheti and An. nili, because the prevalence of other anophelines was too low.
All these five mosquitoes exhibited high global marginality values (>1 in all cases), indicating that they occupied a relatively small portion of the set of environmental conditions (defined by the EGVs) available in Cameroon. Anopheles gambiae and An. funestus exhibited the lowest global marginality values (1.11 and 1.19, respectively), suggesting both species are more 'generalist' relative to the other three anophelines ( Table 2). Global tolerance indices were generally low, ranging from 0.26 in An. arabiensis to 0.69 in An. gambiae, indicating low tolerance towards deviations from the species optimal conditions. Anopheles gambiae and An. funestus had the highest values of tolerance (0.69 and 0.64, respectively), indicating that they are more likely than the other species to colonize sub-optimal habitats. Table 2 shows the coefficients of each EGV on the marginality and first specialization factors for the five most common mosquito species. The marginality factor represents the best combination of all EGVs characterizing the ecological niche of each species at the geographical scale of the spatial reference set. Marginality was particularly important for An. arabiensis and explained 63% of the total model variance. The occurrence of all five major malaria vectors was highly positively correlated with variables related to human activity, including proximity to localities, roads, and croplands (Table 2). Further inspection of the coefficients on the marginality factor allows a clear distinction between predominantly 'savanna' species (e.g. An. gambiae, An. funestus and An. arabiensis), and predominantly 'forest' species (e.g. An. nili and An. moucheti). In the 'savanna' group, occurrences were positively correlated to increasing evapo-transpiration, sunlight exposure, temperature, and wind speed--whose values are typically higher in dry savannas--and negatively correlated to increased water vapour pressure, rainfall and the presence of rainforest. Exactly the reverse trend was observed for both 'forest' species (Table 2). It is noteworthy, however, that An. gambiae, and to a lesser extent An. funestus, revealed intermediate values of the coefficients for these EGVs, in agreement with their lower global marginality and higher tolerance indices.
Inspection of the first specialization factor provided further insights on the ecological niche breadth of the five malaria vectors (Table 2). This factor alone explained 41% and 40% of variation in An. nili and An. moucheti, respec- tively. Both species showed the highest level of specialization for evapo-transpiration, suggesting this EGV has a major impact in limiting habitat suitability for both species to areas of narrow variations in evapo-transpiration, as in the tropical forest. Moreover, low sunlight exposure, high rainfall and water vapour, and presence of the evergreen forest were correlated with the presence of An. nili and An. moucheti. Anopheles arabiensis showed a lower percentage of explained variation (16%) for this first specialization factor, suggesting that the effect on niche breadth for this species is largely distributed across the other specialization factors. Along this factor, the same trend as in An. nili and An. moucheti was observed for An. arabiensis, although in the opposite direction. In addition, An. arabiensis appeared to prefer areas of high wind speed with a high level of specialization for this EGV. Higher wind speed is typically observed in open habitats, which occur at greater frequency in the drier savannas of north Cameroon. Anopheles gambiae and An. funestus showed similar patterns of specialization, the first factor explaining 21% and 16% of variation in each species, respectively. Both species showed low tolerance towards deviation from their optimal rainfall and temperature conditions while showing strong avoidance for rainforest landscapes. This trend was more pronounced for An. funestus than for An. gambiae.

Habitat suitability maps
The number of ENFA factors retained after comparison with a broken-stick distribution to construct Habitat Suitability (HS) maps varied from 2 (explaining 88.5% of total information) for An. moucheti to 6 (explaining Habitat suitability maps for the five major malaria vectors in Cameroon 88.1% of total information) for An. funestus. The median algorithm proposed by Hirzel et al was used to derive HS values for An. gambiae and An. funestus for each cell in the reference area. However, given the high global marginality values observed for An. arabiensis, An. moucheti and An. nili, HS values for these species were derived using the recently proposed area-adjusted median + extremum algorithm, which optimizes the continuous Boyce index for these species (M ae , see [50]). Resulting reclassified maps showing four HS classes (optimal, suitable, marginal and unsuitable) are presented in Figure 2 for each of the five most common malaria vectors in Cameroon.
The HS map of An. gambiae (Figure 2A) identifies a core favourable habitat in the dry savannas of North Cameroon. Habitat suitability decreases when moving northwards to the most arid regions, and southwards to more humid environments. Patches of favourable habitat, however, were also found in areas where the vegetation cover is degraded by human presence, such as the hilly landscapes in the Adamaoua and Western Highlands, and the densely populated area surrounding Yaounde in the forest domain. The humid Atlantic coast and the sparsely populated areas in the evergreen rainforest of the Congo basin in the East are unsuitable for this mosquito, except in close vicinity to inhabited places. A very similar pattern of HS distribution was observed for An. funestus ( Figure 2B), although habitat in the rainforest domain appears of overall lower quality for this species. Anopheles arabiensis, An. nili and An. moucheti had a much more restricted distribution of favourable habitat. The reclassified HS map clearly identified the northernmost, xeric regions of Cameroon as the most suitable habitat for An. arabiensis ( Figure 2C). Patches of suitable habitats were also found at the edge of the Adamaoua and Western Highlands. Further south, across the forested Central plateau and Atlantic belt, the habitat was classified as unsuitable for this species. The HS map for An. moucheti ( Figure 2D) identified areas of suitable habitat that are substantially more restricted than those of the other species: optimal habitat clustered mainly around Yaoundé, with extensions westwards on the Atlantic coast, southwards and eastwards, in the evergreen rainforest of the Congo basin, along the main networks of roads. Northwards, beyond the evergreen forest distribution limit, the habitat was essentially unsuitable for An. moucheti, although patches of marginal and suitable habitat were found in areas where large water reservoirs are found (Adamaoua). Finally, as shown in Figure  2E, the core favorable habitat for An.nili was identified in the densely populated areas south and west of the country, within the rainforest and humid savanna regions, with patchy extensions into the highland areas.  Percentages indicate the amount of total variance explained by each factor. 1 The symbol "+" indicates that the focal species was found in locations with higher values than average for that EGV. The symbol "-" indicates the reverse. The greater the number of symbols, the higher the marginality, with "0" denoting weak marginality. 2 The symbol "*" indicates that the focal species occupied a narrower range of values for the EGV than those available in the reference set (i.e. specialization). The greater the number of symbols, the higher the specialization, with "0" denoting no specialization.
HS maps' accuracy was evaluated by means of 10-fold cross-validations for each of the five species (Table 3). The presence-only evaluators AVI and CVI were around 0.5 for every species, indicating that the HS models were able to discriminate between suitable and unsuitable habitats and that the set of EGVs allowed distinguish specific habitats preferred by each species from the overall habitat available in Cameroon. However, high standard deviations indicated rather low robustness. Boyce's indices provided a more continuous assessment of the model and predictive map accuracy. The values of these indices were positive and high for An. gambiae and An. funestus and somewhat lower for the three other species, especially for An. moucheti. Large standard deviation around most estimates reflected low robustness, especially in the case of the continuous model. However, reclassified HS maps were reliable to predict the distribution of HS throughout Cameroon for most species, as indicated by the high and positive values of the Boyce B4 index which was maximal in the case of An. gambiae and An. funestus ( Table 3). The lowest B4 index, which was associated with a high standard deviation, was that of An. moucheti (Table 3), suggesting that the HS map for this species is to be considered provisional at this stage.

Ecological niche comparisons among species
Results of the discriminant analysis of the ecological niches of each species pairs are shown in Figure 3. This kind of analysis indicates for which eco-goegraphical variables the species differ most. The most important differences were observed between 'savanna' and 'forest' species. Not surprisingly, the presence of evergreen rainforest, high water vapour pressure and high rainfall were always associated with 'forest' species. By contrast, sunlight exposure and temperature were always associated with 'savanna' species. These associations can be easily understood on the basis of the divergence in distribution between 'forest' and 'savanna' species at a macrogeographic scale. More subtle inferences can be proposed for pairs of species that tend to occur in the same locales, such as An. gambiae and An. funestus. In this case, environmental variables spatially discriminating the occurrence of species with similar ecological niches are reflected also in temporal patterns of mosquito population dynamics. For instance, An. gambiae occurrence was correlated to higher values of rainfall and water vapour pressure as compared to An. funestus. On the other hand, An. funestus was associated with higher values of temperature and sunlight exposure ( Figure 3). In nature, the peak in abundance of this pair of species is delayed across the rainy season: An. gambiae peaks at the climax of the rainy season, when rainfall and humidity are higher and temperature and sunlight lower, whereas the peak of An. funestus is further delayed until the beginning of the dry season, when temperature and sunlight are higher and rainfall and humidity lower.

Canonical correspondence analysis
Canonical Correspondence Analysis (CCA, Ter Braak, 1987) was used to analyse the spatial pattern of occurrence of the 10 most common anopheline species in Cameroon: An. gambiae, An. funestus, An. arabiensis, An. nili, An. moucheti, Anopheles paludis, Anopheles ziemanni, Anopheles coustani, Anopheles hancocki and Anopheles pharoensis. A graphical representation of the contribution of each EGV to the global species distribution is given in Figure 4, where the first two canonical axes--explaining 81.8% of the total variance in species distribution--are shown. The EGV that contributed most to species distribution was sunlight exposure, which explained 28% of the variance (Figure 4, F-ratio = 59.8, P = 0.0007), followed by water vapour pressure, evapo-transpiration and rainfall (25%, 21%, and 20%, respectively). The remaining EGVs had a lower impact on species variation.
The first axis, which explained 75.1% of total species variation, was related to environmental variables that reflected a decreasing aridity gradient from left to right: variables associated to mesic conditions were positively correlated with this axis, whereas variables characterizing more xeric habitats were negatively correlated with it (Figure 4). The distribution of species along this axis identified essentially three distinct groups of species. Anopheles arabiensis and An. pharoensis, which are adapted to more arid conditions, mapped on the left of the plot. Anopheles moucheti, An. paludis, An. ziemanni, An. nili, An. coustani, and An. hancocki, mapped on the right side of the ordination space. Anopheles gambiae and An. funestus mapped in Higher mean values indicate higher consistency with the evaluation data sets. The lower the standard deviation, the more robust the predictions. 1 AVI varies from 0 to 1; 2 CVI varies from 0 to AVI; 3 Boyce's indices vary from -1 to 1, with 0 indicating a random model.  the central part of this axis, which--in conjunction with their high tolerance values along this axis (Table 4)-reflects their widespread distribution along this eco-climatic gradient. As previously suggested by the ENFA (Table 2), An. arabiensis and An. moucheti exhibited a narrower ecological distribution compared to other anophelines, with the lowest tolerance values observed on the first axis (Table 4). The tolerance values of An. hancocki and An. pharoensis were relatively large probably because of the limited number of presence points scattered over a number of different ecological settings (see Additional file 1), although the position of these species at both extremes of Axis 1 in Figure 4 testifies for their higher incidence in mesic and xeric conditions, respectively. Finally, the second axis, which explained only 6.7% of total variance, is more difficult to interpret, because it does not represent any unambiguous environmental gradient.

Discussion
One of the main aims of ecologists is to characterize the distribution and abundance of animal populations [57]. Knowledge of where an organism lives is a fundamental requisite for the understanding of its ecology and more detailed analyses about its biology. Nowadays, the availability of accurate environmental data over large spatial extents, together with inexpensive and powerful ways to manipulate and analyse such data, has spurred the development of analytical techniques aimed at predicting species environmental requirements, from which their geographic distribution can be inferred [58].
In tropical Africa, several studies have addressed, by different modelling approaches, the geographic distribution of malaria vectors with the objective to predict malaria transmission risk at the continental scale [12,23,59]. Most studies, however, have relied on collation of published Ordination biplot diagram showing the dispersion of ten malaria vectors and 14 eco-geographical variables on the first two canonical axes of a Canonical Correspondence Analysis Figure 4 Ordination biplot diagram showing the dispersion of ten malaria vectors and 14 eco-geographical variables on the first two canonical axes of a Canonical Correspondence Analysis. Crosses represent the average niche centroid for each mosquito species. In brackets the EGV contribution to total species variance, and the total species variance explained by each canonical axis.
entomological data gathered for purposes different from that of studying the vectors' geographic distribution [23,59,60]. The 'training set' of locations on which such species distribution models are based are generally a biased sample of the sites, where malaria vectors may be effectively present in nature. For example, the absence of a mosquito species in a given locality is likely to reflect the lack of medical entomologists that worked previously in that area, the nature of the collection methods, the seasonal pattern of species abundance, and other environmental or historical accidents, highlighting major limitations of distribution models relying on both accurate presence and absence data. At the continental scale, these limitations may be less compelling, but at higher spatial resolutions (e.g. at country-wide or regional scales) the accuracy of predictive maps based on such records could elude their intentions [59,61]. To overcome some of these limitations, this study assessed habitat suitability for malaria vectors in Cameroon with a species distribution modelling technique based only on presence data gathered from a randomized sampling plan constructed to cover all major bio-geographic domains of the country. Thus, the method used in this study fills a gap in the practical application in both the fields of spatial mapping and statistics and will serve as a stepping-stone for future comparative studies. Areas of improvement in future research will include an ability to employ other presence-only models to compare habitat predicting maps and the accuracy of the predictions [14,62].
In Cameroon, the large diversity of malaria vectors (Table  1) is non-randomly distributed across the country ( Figure  2). Three different analytical approaches were employed (i.e., ENFA, discriminant analysis, and multivariate regressions) to investigate the environmental requirements and to build optimal habitat profiles of the most common malaria vectors in Cameroon [3]. One of the most significant results was the high global marginality value found for all the species concerned, indicating that these mos-quitoes occupied only a specific set of environmental conditions of those available across the country. This is perhaps not surprising considering that marginality values are related to the extent of the spatial reference set, which in this case was constituted by the whole of Cameroon, a highly diversified country covering several different bio-geographic domains. Moreover, as found in other studies [32,63], eco-geographical variables (EGVs) related to human activity (distance to localities and roads) had the most important impact on the ecological niche of anthropophilic malaria vectors. These two EGVs are variables correlated to the density of roads and populated places per unit area, because they take into account the presence of spatial units occupied by localities or roads neighbouring the sampled focal unit. As such, they identify areas where anthropogenic modifications of the environment are greater. This outcome, as well as the anopheline fauna recorded in this study, could be in part related to the collection method, which focused on mosquitoes with domestic resting habits. This is a bias inherent in the fact that the sampling plan targeted those anophelines that are mostly implicated in malaria transmission, which are also those with the most anthropophilic habits (among which the behavioural trait of resting in human dwellings) [21]. Anopheles gambiae and An. funestus are highly anthropophilic and endophilic mosquitoes [33,64,65]; sampling bias is, therefore, not expected to have significantly affected the outcome of habitat suitability maps for these species, as confirmed by the excellent predictive performance of the habitat suitability models for these two species (Table 3). Conversely An. arabiensis is reported to have predominantly exophilic and zoophilic habits in several parts of Africa [66,67], An. nili and An. moucheti can also be highly exophilic [19,68]. Similarly to results obtained by Simard and colleagues [32], species with less anthropophilic behaviour had a weaker correlation with anthropogenic EGVs (Table 2). This difference in the strength of correlation according to degree of anthropophilic behaviour, suggests that sampling bias due to the collection technique used probably did not invalidate unduly the results of the ENFA and habitat suitability maps. It should be noted, however, that model prediction performance was lower in the case of less anthropophilic species (much so in the case of An. moucheti), indicating that there is still scope for improvement of the habitat suitability maps produced with the field data available in this study. Nevertheless, similar ecological species distributions were obtained by the ENFA and canonical correspondence analysis. The latter analytical technique takes into account both species presences and absences, and no EGV related to anthropogenic modifications of the environment was introduced as an explanatory variable. Thus, it can be expected that the distribution patterns observed are likely to be of general value for the less anthropophilic species too. Despite their high global marginality, An. gambiae and An. funestus occurred in sympatry in a wide range of ecological settings ( Figure 4 and Table 4). Habitat suitability maps predicted large patches of optimal habitat for both species from northern to southern Cameroon (Figure 2A, 2B). However, An. gambiae was comparatively more associated with conditions characterized by higher rainfall and humidity (Figure 3), which are characteristics of the equatorial rainforest, explaining why the distribution of optimal habitat in this bio-geographic domain was more extensive for this mosquito compared to An. funestus. It is interesting to note that both species, which showed the lowest global marginality and highest tolerance, have in Cameroon highly structured populations at the genetic level [69][70][71]. Anopheles gambiae is in fact an assemblage of populations belonging to two molecular forms. Simard and colleagues [32] analysed the ecological niche requirements of these evolutionarily diverging ecotypes, showing that when considered as separate entities, the marginality and specialization indices were more extreme than those found in the present work for An. gambiae considered as a single taxonomic entity. Above and beyond the subdivision of An. gambiae in molecular forms, this malaria vector exhibits also an extraordinary degree of chromosomal polymorphism, which can be related to its high capacity to adapt to a wide range of ecological conditions [72,73]. Similarly, An. funestus populations in Cameroon are composed of several chromosomal inversion variants with distinct geographical distributions [69]. The more 'generalist' nature of An. gambiae and An. funestus as taxonomic entities, therefore, could well result from the assemblage of natural populations of genetic variants (karyotypes) each having a specialized ecological niche [63]. Conversely, An. arabiensis, An. moucheti and An.nili had much more restricted and contrasting distributions of suitable habitat ( Figure 2C, 2D, 2E). Anopheles arabiensis was mainly distributed in the most xeric habitats of northern Cameroon that are characterized by high values of evapo-transpiration and sunlight exposure (Table 2). Here, it is frequently found together with the other two major malaria vectors An. gambiae and An. funestus, contributing to high rates of parasite transmission during and soon after the rainy season in the savanna bio-geographic domain [23,61,74]. On the other hand, An. nili and An. moucheti are two 'forest' species, occurring in regions characterized by higher values of water vapour pressure and rainfall (Table 2), as is typically recorded in the equatorial rainforest of southern Cameroon. Both species, together with An. gambiae and, to a lower extent, An. funestus, sustain year-round malaria transmission in the forested regions of Cameroon [68]. Unfortunately, cytogenetic data are as yet not available for these two species to relate their ecological requirements with chromosomal polymorphism.
This study focused on the role that abiotic variables related to climate, topography, or land use have on malaria vector range and distribution, whereas biological processes such as inter-specific competition or predation were not included among the species distribution modelling predictors. Previous studies have revealed how biological interactions, particularly at the larval stage of development, can affect the population dynamics and distribution of anophelines [75,76]. For example, breeding place competition between An. gambiae and An. arabiensis can displace the former in favour of the latter [75]. Contrasting responses to aquatic predators have been considered responsible for generating differences in life-history traits between the two molecular forms of An. gambiae according to the nature of the breeding site [65]. The role that these interactions play in the modulation of mosquito geographic distribution and dynamics certainly warrants further research.
Several studies have reported that human disturbance of the natural environment through the action of irrigation or deforestation can favour the spread and colonization of new areas by efficient malaria vectors, increasing the risk of transmission [29,64,77,78]. In agreement with this view, large patches of optimal/suitable habitat for vectors such as An. gambiae or An. funestus occurred in densely populated areas of intensive farming and around major urban centres, whereas regions of low human population density or uninhabited areas were classified as marginal or totally unsuitable. This suggests that global environmental changes, including deforestation, urbanization, or land use conversion for agricultural purposes, as well as the ongoing demographic surge that Africa is currently experiencing are likely to impact on vector distribution and malaria epidemiology in the times to come. Future research should consider the dynamic nature of mosquito population ecology, including population genetic analyses, to understand the evolution of species ranges and current trends in malaria transmission risk.
The presence of a highly differentiated malaria vector system occurring in a given geographical area, as observed in Cameroon, can clearly have a profound impact on the nature and intensity of transmission [19,29]. In this context, fine-grained mapping of the vectors' distribution together with the identification, characterization and ranking of their ecological requirements, as well as of the ecological determinants to which mosquitoes respond, is of great interest to assess and predict disease transmission risk. Such knowledge might allow focusing vector control efforts in areas and at times where the target vector species are most amenable to control, and improve both efficacy and cost-effectiveness of disease control through vector control interventions [62,79].