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Effects of Wenqingyin on the Proliferation and Differentiation of HaCaT Cells

Objective: To investigate the effects of Wenqingyin on the proliferation and differentiation of HaCaT cells. Methods: Cultured HaCaT cells in vitro were used to test the effects of Wenqingyin in various durations on the Proliferation and Differentiation of HaCaT Cells. The cell proliferation was assessed with CCK-8 method,the changes of filaggrin(FLG) mRNA expressions by semi-quantitative reverse transcription PCR. Results: The proliferation of HaCaT cells was inhibited by the treatment with Wenqingyin with the minimum inhibiting concentration 5 mg/m L,and the inhibition rate rose with the increase of the dosage,which was dose-dependent. With the use of Wenqingyin,the mRNA expression of FLG was higher than that of the control group. Conclusion: Wenqingyin can inhibit the proliferation and induce the differentiation of keratinocytes.