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The basophil activation test in the diagnosis of allergy: technical issues and critical factors

Background:  The basophil activation test (BAT) is a widely validated and reliable tool especially for the diagnosis of hymenoptera venom allergy. Nevertheless, several pitfalls have to be considered and outcomes may differ because of diverse in‐house protocols and commercially available kits. We aimed to identify the factors that may influence results of the CD63‐based BAT.