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Modulations of NA+ transport during the cell cycle of neuroblastoma cells

Na+ transport properties of neuroblastoma (clone Neuro‐2A) cells have been characterized both in exponentially growing cells and during the cell cycle. In exponentially growing cells, Na+ influx followed the kinetics for a one‐compartment system with an influx rate of 9.54 ± 0.96 nmoles/minute/106 cells, equilibrium being reached within 2 minutes. The initial rate of influx in the presence of ouabain (5 mM), a concentration completely inhibiting the (Na+‐K+)ATPase and thus backflux of tracer, was 11.33 ± 1.50 nmoles/minute/106 cells and, within error, the same as in the absence of ouabain, provided determinations were made within 3 minutes of ouabain addition.