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[Applying competitive polymerase chain reaction to the detection of hepatitis B virus DNA].

OBJECTIVE
To reduce the rate of accidental false negative result in the HBV DNA PCR test on clinical serum samples.


METHODS
A competitive polymerase chain reaction (C-PCR) was used to decrease the false negative ratio. In the C-PCR, a constructed inner control DNA was added for co-amplification with the HBV target DNA.


RESULTS
In a 20 microl C-PCR system, about 60 to 200 copies of inner control DNA could give apparent co-amplification signal band after electrophoresis on a 2% agarose gel. Five of 120 samples of clinical serum (4.2%) could not be amplified.


CONCLUSION
C-PCR has the advantage of yielding information on false negative in the HBV DNA PCR assay of clinical serum samples.