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Synergistic effect of vascular endothelial growth factor and granulocyte colony‐stimulating factor double gene therapy in mouse limb ischemia

Vascular endothelial growth factor (VEGF) has mostly been tested to treat ischemic diseases, although the outcomes obtained are not satisfactory. Our hypothesis is that the local transient expression of VEGF and stem cell mobilizer granulocyte colony‐stimulating factor (G‐CSF) genes in ischemic limbs can complement their activities and be more efficient for limb recovery.