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Oral-fluid samples for surveillance of commercial growing pigs for porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 infections

Materials and methods: Oral-fluid and serum samples were collected from one barn on each of three PRRSV-infected finishing sites. Six pens per barn (20 to 30 pigs per pen) were sampled repeatedly, beginning when the pigs entered the facilities (3 weeks of age), and then at 5, 8, 12, and 16 weeks of age. Serum samples were tested using a commercial PRRS ELISA. Both serum and oral-fluid samples were tested for PRRSV by quantitative reversetranscriptase polymerase chain reaction (PCR), and oral fluids were tested for PCV2 by quantitative PCR.