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Fluorometric determination of salicylic acid in aspirin products including noninterfering, interfering, and buffered substances.

A rapid, fluorometric method for the determination of salicylic acid in aspirin products in combination with noninterfering fluorescent substances, such as phenyltoloxamine citrate, glyceryl guaiacolate, and caffeine, interfering fluorescent materials, such as hydrogenated castor oil (Castorwax) and salicylamide, and buffering agents, such as aluminum glycinate and magnesium carbonate, gave accurate and precise results. The procedure is based on a simple dissolution of the crushed tablet in a pH 4.0 buffer solution and reading of the filtered solution at activation and fluorescent wavelengths of 310 and 410 mμ, respectively. An additional operation of a chloroform and buffer solution extraction is necessary when interfering substances are present. In the case of salicylamide, a constant background reading has to be subtracted; however, an accuracy of 95.5% was established. For nonaspirin salicylates in buffered-aspirin products, the procedure gave results similar to those reported by Guttman and Salomon.