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Biological effects of autoantibodies against α1‐adrenoceptor in patients with primary hypertension

The aim of the present study was to evaluate the biological effects of the anti‐α1AR‐autoantibodies from the sera of patients with primary hypertension. The anti‐α1AR‐autoantibodies were detected by SA‐ELISA with the synthetic peptide corresponding to the sequence of the second extracellular loop of human α1AR. The IgGs from the positive sera of patients with primary hypertension were purified by affinity chromagraphy, and the effects of the purified IgGs on the tension of isolated thoracic aortic rings of rats were observed. The autoantibodies (10−9~10−5mol/L) increased tension of isolated thoracic aortic rings of rats with concentration‐dependent matter (maximal contraction from 19.27±2.18% to 137.51±4.62%, n=12, p<0.01), which is similar to the α1AR agonist phenylephrine (PE) and independent with the endothelial cells. But the effect can not occurrence with IgGs from the negative sera, and can not be modified by pretreating aortic ring with Prasozin (α1AR antagonist) or synthetic peptide corresponding to the sequence of the second extracellular loop of human α1AR in high concentration. Our results demonstrated for the first time that the anti‐α1‐adrenoceptor autoantibodies in the sera of patients with primary hypertension exhibited remarkable effects of vascular contraction, and suggesting that the involvement of immunologic mechanism by cardiac receptor antibodies in the pathogenesis of primary hypertension.