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TOTAL REFLECTION X-RAY FLUORESCENCE ANALYSIS OF CISPLATIN PLATINUM IN HUMAN CANCEROUS TISSUES OBTAINED BY BIOPSY

Total reflection X-ray fluorescence analysis (TXRF) has been successfully used to determine trace amounts of Cisplatin platinum in biopsy samples. The minimum detection limit of Pt by TXRF analysis using monochromatic Mo Kα radiation with a LiF (200) crystal was 0.01ppm, and the minimum quantification limit was 0.1ppm for the biological samples. Biopsy tissues and blood serum samples were taken from patients with advanced bladder cancers. Variations of Pt concentration at tumor tissue and in blood serum of the patient with time were clarified for both types of samples. This study indicates that TXRF is a most suitable technique for the analysis of trace platinum in biopsy samples.