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Inactivation of Hepatitis B and Hutchinson Strain Non‐A, Non‐B Hepatitis Viruses by Exposure to Tween 80 and Ether 1

Titrated stocks of hepatitis B virus and Hutchinson strain non‐A, non‐B hepatitis virus were diluted in normal serum to contain, respectively, ≥106 and ≥104 chimpanzee infectious doses (CID50) per milliliter and exposed to 1% Tween 80 and 20% ether at 4°C for 18 h. After evaporation of the ether, the treated sera were each inoculated into two chimpanzees. The animals remained free of serologic and biochemical evidence of hepatitis during a 6‐month follow‐up period, and were then shown to be susceptible to infection by challenge with the original untreated inocula.