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[Development and characterization of monoclonal antibodies against VP1 protein of AsiaI type foot-and-mouth virus].

AIM
To prepare the monoclonal antibodies against VP1 protein of type AsiaI foot-and mouth disease virus (FMDV) and identify the characterization.


METHODS
Three cell of hybridization that strains secreted monoclonal antibody(mAb) against type AsiaI FMDV were produced by fusing mouse myeloma cells (Sp2/0) with spleen cells from BALB/c immunized with the purified recombinant VP1 protein.


RESULTS
The three hybridized cell lines reacted with cattle type AsiaI FMDV, the titres of ascetic fluids of the three mAbs ranged from 1:10(5) to 1:10(6) indirect ELISA showed. Among the three mAbs,1B8 belonged to IgG1, 5E1 and 5E2 pertained to IgG2a. Besides, the three strain antibodies stably excreted antibodies and reacted with type AsiaI FMDV.


CONCLUSION
VP1 protein of FMDV could replace FMDV can be use to replace FMDV to prepare mAbs.