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Cholesterol Tethered Poly(DL-Lactic Acid) for Promoting Osteoblast Attachment and Growth

In poly(DL-lactic acid)-cholesterol oligomers (LC), a novel cholesterol modified poly(DL-lactic acid) (PLA), was synthesized via bulk polymerization of DL-lactide using cholesterol initiator. Gel permeation chromatography (GPC) and 1H nuclear magnetic resonance spectroscopy (1H-NMR) results indicated a narrow molecular weight distribution of poly (DL-lactic acid)-cholesterol oligomers (LC). Mouse MC3T3 osteoblast-like cells were selected as a model system to test the cell behavior of cholesterol modified PLA substrates. The osteoblast attachment, proliferation, and viability revealed that the cholesterol modified PLA was significantly osteoblast compatible and may have potential as a bone tissue engineering material.