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Neutrophils Movement in Vitro

Abstract: Ineffectiveness of the motility of neutrophils is observed in some immunodeficiency disorders. There is a need to find an adequate description and means to evaluate the activity of neutrophils. Early studies describe an individual cell behavior by the cell motility index vector with three components: penetration, directionality, and expansivenesses. Movement quantification has been improved by extending the description of cell activity to two stages of classification. In the first stage, one minute of each cell's behavior is classified into one of five types of cell behavior, using the k‐NN method. In the second stage, the sample level that corresponds to the final classification, the Shapiro‐Francio test has been used. The proposed new method allows us to describe differences between a normal child and a Chediak‐Higashi syndrome patient.