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Optimization of Vasculogenesis within Naturally-Derived, Biodegradable Hybrid Hydrogel Scaffolds

IntroductIon: Cellular ingrowth and neovascularization of acellular tissue-regeneration scaffolds represent the ratelimiting steps of permanent integration.1 In previous work, we have demonstrated that naturally-derived, biodegradable hybrid hydrogel scaffolds fabricated from a 10:1 w/w combination of alginate and type I collagen allow for maximal human umbilical vein endothelial cell (HUVEC) adherence and invasion in an in vitro wound healing model. We next sought to stimulate endothelial tubule formation within these scaffolds.