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Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis

Thyroid cancer is one of the most common malignant tumors, and the mortality rate associated with thyroid cancer has been increasing annually. Curcumin has been reported to exert an antitumor effect on papillary thyroid cancer (PTC), and the identification of additional mechanisms underlying the anticancer effect of curcumin on PTC requires further investigation. The present study aimed to explore the effects of curcumin on the viability, migration and invasion of PTC cells. TPC-1 cells were incubated with different concentrations of curcumin, and then, cell viability, migration and invasion, and wound healing were examined by CCK-8, Transwell and wound healing assays, respectively. Subsequently, microRNA (miR)-301a-3p mimics, miR-301a-3p inhibitors and signal transducer and activator of transcription (STAT)3 overexpression vector were transfected into TPC-1 cells, and cell viability, migration, and invasion were reassessed in these transfected cells. Matrix metallopeptidase (MMP)-2, MMP-9, epithelial-mesenchymal transition (EMT)-related markers, and Janus kinase (JAK)/STAT signaling pathway components were assessed by western blot analysis. Curcumin significantly inhibited cell viability, migration and invasion and downregulated MMP-2, MMP-9 and EMT marker expression. Additionally, curcumin decreased STAT3 expression by upregulating miR-301a-3p expression, and the inhibition of miR-301a-3p and the overexpression of STAT3 reversed the effects of curcumin on cell viability, migration and invasion, and MMP-2, MMP-9 and EMT marker expression in TPC-1 cells. Furthermore, curcumin suppressed the JAK/STAT signaling pathway through the miR-301a-3p/STAT3 axis. The data of the present study indicated that curcumin could inhibit the viability, migration and invasion of TPC-1 cells by regulating the miR-301a-3p/STAT3 axis. These findings may provide a possible strategy for the clinical treatment of PTC.


Introduction
Thyroid cancer is one of the most common malignant tumors, accounting for approximately 95% of all endocrine cancer cases and 3% of all other cancer cases (1). The age-standardized mortality rate worldwide was 0.35/10 5 in 2015 (2). According to the American Cancer Society, in the United States, ~52,890 new cases of thyroid cancer were estimated in 2020, the number of female patients was projected to reach 40,170 cases, and the number of new cases in males was estimated to reach 12,720 (1). Thyroid cancer is histologically classified into 5 types. Papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC) are the most common well-differentiated thyroid carcinomas, and these types of cancer generally have satisfactory prognosis and can be efficiently treated by conventional therapies (3). However, anaplastic thyroid cancer (ATC), poorly differentiated thyroid cancer (PDTC) and medullary thyroid cancer (MTC) may differentiate into more aggressive diseases that do not respond adequately to conventional therapies, ultimately leading to poor patient survival (4). Surgery and endocrine therapies play vital roles in the treatment of thyroid cancer. However, some patients have increased tumor invasiveness, which causes poor therapeutic efficacy and great pain (5). Therefore, an effective treatment or specific therapeutic strategy is urgently needed for the improved management of this malignancy.
Herbs (also known as botanicals), vitamins, minerals and probiotics have been used as complementary and alternative medicines in the treatment of cancer (6). Traditional Chinese medicine (TCM) has been reported to be an effective method

Curcumin inhibits the viability, migration and invasion of papillary thyroid cancer cells by regulating the miR-301a-3p/STAT3 axis
for treating malignant tumors (7,8). TCM has been revealed to reduce the production of drug resistance and provoke less damage and fewer side effects in patients. Curcuma longa (C. Longa) Linn is a Zingiberaceae plant, and its antitumor effects have been reported in thyroid (9), lung (10) and colorectal cancer cells (11). Curcumin is a main phenolic active compound in C. Longa Linn, and it has numerous pharmacological activities such as anti-inflammatory (12), antibacterial (13) and, especially, anticancer activities (14). The anticancer effects of curcumin are due to targeting a wide range of cellular and molecular pathways involved in cancer pathogenesis (15). For example, curcumin has been revealed to induce endoplasmic reticulum stress-associated apoptosis in human PTC cells via disruption of intracellular calcium homeostasis (16) and to affect PTC cells by targeting the JAK/STAT3 signaling pathway (17). Thus, the identification of additional mechanisms underlying the anticancer effect of curcumin on PTC requires further investigation.
MicroRNAs (miRNAs or miRs) are a group of noncoding RNAs that have been developed as novel biomarkers for the diagnosis and treatment of thyroid carcinoma (18). In previous studies, a variety of tumor-related miRNAs have been reported to participate in the development and diagnosis of cancer, including PTC (19,20). Studies have demonstrated that miRNAs can act as either oncogenes or tumor suppressors in cancer. For instance, miR-301a-3p suppressed the progression of hepatocellular carcinoma by targeting VGLL4 (21). miR-486-5p was revealed to be highly expressed in PTC and promoted PTC cell invasion by influencing epithelial-mesenchymal transition (EMT) via targeting of KIAA1199 (22). However, miR-188-5p was demonstrated to be expressed at low levels in PTC, and restoration of its expression suppressed PTC cell proliferation (23). Interestingly, a previous study reported that curcumin exerted anticancer effects by regulating miRNAs (24), and caused a strong and significant reduction in miR-221, miR-222 and miR-21 (25). Functional miRNAs have been indicated to regulate target genes or proteins (26). The constitutive activation of Janus kinase/signal transducer and activator of transcription (JAK/STAT) has been revealed to play a vital role in the cell biological behavior and immune response associated with cancer progression (27,28). The JAK/STAT signaling pathway has been demonstrated to be involved in the oncogenesis related to thyroid cancer and is considered to be a potential prognostic biomarker and therapeutic target (17,29). In addition, emerging evidence has indicated that STAT3 is related to proliferation, migration and invasion in PTC (30)(31)(32). Interestingly, a recent study reported that curcumin inhibited the viability and migration of PTC cells by targeting the JAK/STAT3 signaling pathway (17), which may be a potential therapeutic target for PTC.
In the present study, the antitumor effects of curcumin on a PTC cell line (TPC-1) were examined and the molecular mechanisms underlying the curcumin-mediated effects in TPC-1 cells were investigated. Our results revealed that the miR-301a-5p/STAT3 axis may be an important signaling pathway in mediating the antitumor effects of curcumin on TPC-1 cells. The present study may provide new insights into the antitumor effects of curcumin in PTC.

Materials and methods
Cell lines and cell culture. A PTC cell line (TPC-1) was obtained from Shanghai Huiying Biological Technology Co., Ltd. A human thyroid cancer cell line (BCPAP-R, the BRAF mutation) and a human normal cell line (Nthy-ori3-1) were obtained from the Chinese Academy of Medical Sciences. The cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich; Merck KGaA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Inc.) in an incubator containing 5% CO 2 at 37˚C (Thermo Fisher Scientific, Inc.) (33).
Cell-Counting Kit-8 (CCK-8) assay. Cell viability was detected by the CCK-8 assay (Beyotime Institute of Biotechnology) according to the manufacturer's instructions, as previously described (17). Briefly, TPC-1 and BCPAP cells were seeded into 96-well plates at a density of 1x10 4 cells/well, cultured overnight and treated for 24 h. After treatment, 10 µl of CCK-8 solution was added to each well of the 96-well plate and incubated at 37˚C for 4 h. The optical density (OD) was measured at 450 nm with a microplate reader (BioTek Instruments, Inc). The half-maximal inhibitory concentrations (IC 50 ) were estimated based on the dose-response curve.
Wound healing assay. TPC-1 and BCPAP cells were seeded in 6-well plates (~70-80% confluency) and cultured at 37˚C for 48 h until they reached full confluence. The full confluent monolayers were scratched with 200-µl pipette tips. Then, the cells were cultured in DMEM supplemented with 10% FBS (34). The wound area was recorded via light microscopy observations at 0 and 24 h and images were captured using the Lionheart™ FX automated live cell imager (BioTek Instruments, Inc.).
Transwell assay. TPC-1 and BCPAP cell suspensions were counted and then diluted to 5x10 4 cells/ml. Transwell chambers were placed into a 24-well plate (Costar; Corning, Inc.), and the cell suspension was seeded into the apical chambers; DMEM medium (0.5 ml) containing 10% FBS was added to the basolateral chambers, and the plates were incubated in an incubator containing 5% CO 2 at 37˚C for 24 h. The migrating cells on the basal side of the membranes were treated with paraformaldehyde (4%) at 37˚C for 15 min, and crystal violet (0.1%) was used to stain the cells at 37˚C for 5 min, followed by washing with PBS. Images of each chamber were captured using a light microscopy Lionheart™ FX automated live cell imager, and the number of cells in each field was used to obtain an average number. For the cell invasion assay, the Transwell chambers were precoated with Matrigel (BD Biosciences) at 37˚C for 15 min, and a similar protocol was followed.

Real-time quantitative polymerase chain reaction (RT-qPCR).
Total RNA was isolated from cells using TRIzol ® solution (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. First-strand cDNA was synthesized from the total RNA using the PrimeScript™ RT Reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacturer's protocol. The expression level of the miR-301a-3p miRNA was assessed using an RT-qPCR system (ABI Prism 7300; Applied Biosystems; Thermo Fisher Scientific, Inc.) with 2X TaqMan Fast qPCR Master Mix (Sangon Biotech, Co., Ltd.), according to the manufacturer's protocol. Specific primers were designed and synthesized by Sangon Biotech Co., Ltd. The relative miRNA levels were calculated using the 2 -ΔΔCq method (35) and normalized to the U6 level. All the samples were analyzed in at least 3 parallel reactions. The primer sequences were as follows: miR-301a-3p (Accession number: NR_029842) forward, 5'-ACA CTC CAG CTG GGC AGT GCA ATA GTA TTG TC-3' and reverse, 5'-CTC AAC TGG TGT CGT GGA-3'; and U6 (Accession number: NR_004394) forward, 5'-CTC GCT TCG GCA GCA CATA-3' and reverse, 5'-AAC GAT TCA CGA ATT TGC GT-3'. The following thermocycling conditions were used: Initial denaturation at 95˚C for 3 min; 40 cycles at 95˚C for 5 sec, 60˚C for 15 sec and 72˚C for 30 sec.
Bioinformatics and luciferase reporter assays. StarBase online software was used to predict the targeted binding sites of miRNAs in mRNAs. Subsequently, the targeted binding relationships between miR-301a-3p and STAT3 were predicted. A pmirGLO Dual-Luciferase Expression Vector (Promega Corporation) that included wild-type (WT) or mutant (MUT) 3'-untranslated region (UTR) binding sites of the STAT3 sequence, named WT-STAT3 or MUT-STAT3, respectively, was constructed and co-transfected with miR-301a-3p mimics, inhibitors and the corresponding negative controls into 293T cells (Bank of Type Culture Collection of the Chinese Academy of Sciences). A total of 48 h after transfection, the results were obtained using a Dual-Luciferase Reporter Assay System (Promega Corporation), according to the manufacturer's protocol. Firefly luciferase activity was normalized to Renilla luciferase activity and all experiments were performed in triplicate.
Statistical analysis. All the experiments were performed with three independent biological replicates. The data are expressed as the mean ± standard deviation (SD). The IC 50 values in cells were calculated following non-linear regression on the dose-response curves. Multigroup comparisons were performed using one-way analysis of variance (ANOVA) followed by Tukey's post hoc test, and comparisons between two groups were performed using unpaired t-tests. P<0.05 was considered to indicate a statistically significant difference and the analyses were conducted with SPSS 20.0 software (IBM Corp.).

Results
Curcumin suppresses PTC cell viability. The antitumor effect of curcumin on PTC cells was examined by CCK-8 assay. TPC-1 and BCPAP-R cells were treated with different concentrations of curcumin (0, 2.5, 5, 10, 20 and 40 µM) for 24 h. As demonstrated in Fig. 1A and C, curcumin significantly reduced the viability of the PTC cells in a concentration-dependent manner. The data indicated antitumor activity, and based on the dose-response curve, the IC 50 of curcumin was approximately 23.31 and 26.43 µM for the TPC-1 and BCPAP-R cells, respectively, at 24 h ( Fig. 1B and D). Hence, cells treated with 23.31 and 26.43 µM curcumin for 24 h were used for the following experiments.

Curcumin inhibits cell migration, invasion and EMT.
To further explore the functions of curcumin in PTC cells, its effects on the migration and invasion of TPC-1 and BCPAP-R cells were examined. Curcumin treatment at a concentration of 23.31 µM or 26.43 µM for 24 h significantly inhibited cell migration and invasion compared with the control treatment ( Fig. 1E-H;  P<0.001, respectively). Similarly, the wound healing assay demonstrated that curcumin significantly reduced cell migration ( Fig. 1I and J; P<0.01, respectively). The protein levels of MMP-2 and MMP-9 were evaluated in TPC-1 cells treated with curcumin. Curcumin significantly reduced the protein expression of MMP-2 and MMP-9 ( Fig. 1K; P<0.01, respectively). In addition, EMT-related markers (N-cadherin, E-cadherin, vimentin and fibronectin) were also detected by western blot analysis. As revealed in Fig. 1L, compared with the control treatment, curcumin treatment decreased the protein expression levels of N-cadherin (P=0.047), vimentin (P=0.021) and fibronectin (P=0.048) but increased the protein expression level of E-cadherin (P<0.01). These data indicated that curcumin suppressed cell migration and invasion as well as MMP-2, MMP-9 and EMT marker expression in PTC cells.
Curcumin inhibits cell viability, migration, invasion and EMT by upregulating miR-301a-3p. The expression of miR-301a-3p in thyroid carcinoma was validated using starBase. As revealed in Fig. 2A, the expression of miR-301a-3p was downregulated in thyroid carcinoma (P= 0.043) and significantly downregulated in TPC-1 and BCPAP-R cells compared with Nthy-ori-1 cells (Fig. 2B; P<0.001). Next, to explore the potential mechanism by which curcumin drives the progression of PTC cells, the expression of miR-301a-3p was detected after treatment with different concentrations of curcumin (0, 5, 10, 20 and 40 µM) for 24 h. As observed in Fig. 2C, curcumin markedly increased the expression of miR-301a-3p in a concentration-dependent manner. To further examine the function of miR-301a-3p in TPC-1 cells, cell viability, migration and invasion were analyzed. As anticipated, transfection of miR-301a-3p inhibitors significantly decreased the expression of miR-301a-3p compared with transfection of the NC group ( Fig. 2D; P<0.001). The cell viability of TPC-1 cells transfected with the miR-301a-3p inhibitors was higher than that of the of the TPC-1 cells transfected with NC (P=0.048), and the viability was decreased after curcumin treatment (P<0.001) but increased after miR-301a-3p inhibitor transfection ( Fig. 2E; P<0.001). Moreover, the addition of the miR-301a-3p inhibitors significantly increased the migration and invasion abilities of the TPC-1 cells (P=0.027 and P=0.021, respectively) compared with the NC group, and cell migration and invasion were inhibited after curcumin treatment (P<0.001, respectively); these effects were reversed by the transfection of miR-301a-3p inhibitors ( Fig. 2F and G; P<0.001 and P<0.01, respectively). Similarly, the wound healing assay demonstrated that curcumin significantly inhibited wound closure, which was enhanced by the transfection of miR-301a-3p inhibitors ( Fig. 2H; P<0.001).
miR-301a-3p directly targets STAT3. The bioinformatics software starBase was used to identify potential targets of miR-301a-3p, and STAT3 was selected due to its role in cancer metastasis. To confirm the interaction between miR-301a-3p and STAT3, a luciferase reporter vector containing the WT or MUT 3'UTR of STAT3 was constructed (Fig. 4A). Overexpression of miR-301a-3p significantly suppressed the luciferase activity of the WT reporter vector (Fig. 4B; P<0.001) but did not affect the luciferase activity of the mutant reporter vector in 293T cells. Furthermore, transfection of miR-301a-3p mimics resulted in a significant increase of the expression of miR-301a-3p in TPC-1 cells compared with the NC mimics group (Fig. 4C; P<0.01), and overexpression of miR-301a-3p also significantly suppressed the protein expression level of STAT3 in TPC-1 cells (Fig. 4D; P<0.001). Collectively, the aforementioned results indicated that miR-301a-3p directly targeted the STAT3 gene.

Curcumin inhibits cell viability, migration and invasion by
regulating the miR-301a-3p/STAT3 axis. The next aim was to determine the functional interaction of miR-301a-3p and STAT3 during the curcumin-mediated inhibition of TPC-1 cell viability, migration and invasion. Firstly, the successful overexpression of STAT3 was confirmed through the transfection of pc-STAT3 vector ( Fig. 5A; P<0.001). Subsequently, the expression of STAT3 was significantly suppressed by curcumin treatment (P<0.001), which was significantly reversed by STAT3 overexpression in TPC-1 cells (P=0.040), and miR-301a-3p overexpression also significantly suppressed STAT3 protein expression ( Fig. 5B; P<0.001). Overexpression of miR-301a-3p significantly decreased cell viability compared with NC group (P<0.001); in addition, cell viability was also significantly suppressed by curcumin treatment (P<0.001), and this effect was significantly reversed by STAT3 overexpression in TPC-1 cells (Fig. 5C; P<0.001). Moreover, cell migration and invasion were inhibited by miR-301a-3p overexpression and curcumin treatment (P<0.001, respectively), and the overexpression of STAT3 alleviated the inhibitory effect of curcumin on cell migration and invasion ( Fig. 5D and E; P=0.018 and P=0.028, respectively). Similarly, the wound healing assay demonstrated that the miR-301a-3p mimics and curcumin significantly reduced cell migration (P<0.001), and the overexpression of STAT3 reversed the inhibitory effect of curcumin on cell migration ( Fig. 5F; P=0.047). These data indicated that curcumin suppressed TPC-1 cell migration and invasion by regulating the miR-301a-3p/STAT3 axis.
Curcumin inhibits MMP-2, MMP-9 and EMT marker expression and suppresses the JAK/STAT pathway by regulating the miR-301a-3p/STAT3 axis. The protein expression levels of MMP-2, MMP-9 and EMT-related markers (N-cadherin, vimentin and fibronectin) were decreased in the miR-301a-3p mimic and curcumin groups compared with the NC group (P<0.001, respectively), and the overexpression of STAT3 reversed the inhibitory effect of curcumin on protein expression ( Fig. 6A and B; P<0.05). Conversely, the E-cadherin protein expression was increased after the miR-301a-3p and curcumin treatments (P<0.001), but the overexpression of STAT3 reversed the effect of curcumin on the promotion of protein expression (P=0.028). STAT3 is one of the pivotal proteins involved in the JAK/STAT signaling pathway (38). Therefore, the effects of curcumin on the JAK/STAT signaling pathway were investigated. As demonstrated in Fig. 6C, the levels of phosphorylated JAK1, JAK2, JAK3, STAT1 and STAT2 were significantly suppressed by curcumin treatment (P<0.001, respectively), which was effectively reversed by the overexpression of STAT3 in TPC-1 cells (P<0.05), and the overexpression of miR-301a-3p also significantly suppressed the levels of phosphorylated JAK1, JAK2, JAK3, STAT1 and STAT2 (P<0.001, respectively). Collectively, these data indicated that curcumin inhibited MMP-2, MMP-9 and EMT-related marker expression, and suppressed the JAK/STAT pathway by regulating the miR-301a-3p/STAT3 axis.

Discussion
Curcumin has been revealed to serve as a potent antitumor drug for the treatment of multiple cancers, including PTC (14,39). It has already been suggested that curcumin suppresses the survival, invasion and migration of cancer cells (39). In  Curcumin has been demonstrated to possess antitumor, anti-inf lam mator y, antioxidant and antim icrobial properties (43)(44)(45). Typically, curcumin is involved in multiple anticancer processes (46). Results of a previous study have demonstrated that miRNAs may act as protective regulators of curcumin antitumor activity (47). The present study revealed that miR-301a-3p was expressed at lower levels in PTC and was upregulated by curcumin. miR-301a-3p has been revealed to regulate multiple biological processes, including cell proliferation, differentiation, biological development and numerous metabolic processes in organisms (48,49). Furthermore, it was demonstrated that inhibition of miR-301a-3p reversed the inhibitory effects of curcumin on the viability, migration and invasion of TPC-1 cells. Thus, miR-301a-3p was identified as a protective regulator in PTC cells. The curcumin-mediated regulation of miRNA expression, including the activation/inactivation of miRNA transcription, the regulation of associated signaling pathways and the methylation/demethylation of miRNAs, is complex (50). Spadotto et al revealed that extensive methylation of complexes led to downregulated miR-301a expression (51). Curcumin has been demonstrated to cause epigenetic modulation of miR-203 expression by demethylating the miR-203 promoter in bladder cancer (52). Curcumin could increase miR-301a-3p expression via decreased methylation of the complex and/or miR-301a-3p promoter in PTC.
Curcumin has been revealed to inhibit cell proliferation, migration and invasion by activating the JAK2/STAT3 signaling pathway, as it has been reported for different human malignancies, such as retinoblastoma and ovary and thyroid cancers (17,53,54). Our study further demonstrated that curcumin inhibited the JAK/STAT signaling pathway by upregulating miR-301a-3p expression and that miR-301a-3p directly targeted STAT3. These observations indicated that miR-301a-3p was a suppressor of STAT3 protein expression and the JAK/STAT signaling pathway in TPC-1 cells. STAT3 is considered to be one of the most common oncogenes in human cancers and plays a key role in PTC progression (17,55). It was further demonstrated that curcumin and miR-301a-3p mimics reduced the levels of phosphorylated JAK1, JAK2, JAK3, STAT1 and STAT2 via the targeting of STAT3 by miR-301a-3p, whereas the overexpression of STAT3 abolished the suppressive effect of curcumin on this signaling pathway. Thus, the miR-301a-3p/STAT3 axis was identified as an efficient regulator of JAK/STAT signaling pathway in TPC-1 cells.
It has been reported that curcumin regulates EMT in human cancers, including PTC (41,56,57). Notably, EMT is considered to be a critical event in cancer cell migration and invasion, and curcumin has been reported to inhibit PTC cell migration and invasion (25,58,59), indicating that curcumin inhibits PTC cell migration and invasion by inhibiting EMT. In the present study, it was demonstrated that curcumin inhibited EMT, migration and invasion of PTC cells. MiR-301a-3p inhibitors increased EMT and reversed the inhibitory effects of curcumin in EMT of TPC-1 cells. These observations indicated that curcumin inhibited EMT by upregulating miR-301a-3p expression. It has been reported that curcumin suppressed EMT through the miR-200c/EPM5 axis and the ERK5/AP-1 and TET1-NKD-Wnt signaling pathways (60)(61)(62). Furthermore, our results indicated that the targeting of STAT3 by miR-301a-3p and the overexpression of STAT3 also reversed the inhibitory effects of curcumin on EMT in TPC-1 cells. Thus, it was revealed that curcumin inhibited EMT by regulating the miR-301a-3p/STAT3 axis.
In our study, it was demonstrated that curcumin inhibited STAT3 expression. Furthermore, the overexpression of STAT3 reversed the inhibitory effects of curcumin on the viability, migration and invasion of TPC-1 cells. It has been reported that suppression of STAT3 activity or expression inhibits human cancer progression (55,63,64). Curcumin has been demonstrated to promote PTC cell apoptosis by increasing DNA damage, endoplasmic reticulum stress and the PI3K/Akt signaling pathway (16,42,59). These observations demonstrated that targeting STAT3 is a potential therapeutic target for PTC. In the present study, it was revealed that curcumin inhibited the viability of TPC-1 cells by regulating the miR-301a-3p/STAT3 axis. An increasing number of studies has reported that curcumin leads to cell cycle arrest at the G2/M phase in PTC cells (40,42,65). Therefore, it was theorized that curcumin inhibited the viability of PTC cells by affecting the G2/M phase of the cell cycle. It has been reported that curcumin promotes PTC cell apoptosis and inhibits PTC cell migration and invasion (16,25,(40)(41)(42)(57)(58)(59)65). A recent study reported that curcumin mediated BCPAP-R and TPC-1 cell apoptosis and stemness by targeting the JAK/STAT3 signaling pathway (17). Notably, the present study revealed that curcumin downregulated STAT3 expression by upregulating miR-301a-3p expression in TPC-1 cells. Previous studies have demonstrated that STAT3 promotes tumour progression in PTC (66)(67)(68). The results of the present study provided strong evidence that curcumin decreases the expression of STAT3 and suppresses the viability, migration and invasion of PTC cells via upregulating miR-301a-3p.
However, the potential role and underlying mechanism of curcumin in PTC was only investigated in vitro, and several limitations should also be considered. Curcumin affects miRNAs that have been reported to be involved in human cancer progression (15). Curcumin may affect the expression of additional miRNAs in PTC cells (40). In future studies, microarray assays may be used to profile the miRNAs that could be regulated by curcumin in TPC-1 cells. In addition, the present study only selected STAT3 as a target of miR-301a-3p and TPC-1 cells as the cell model for further study, but miR-301a-3p may also target other genes. Consequenlty, future studies may investigate more targets of miR-301a-3p to further reveal the underlying antitumor mechanisms of the antitumor effects of curcumin on additionl types of PTC cells. Moreover, whether the use of curcumin with chemotherapeutic reagents currently used to treat PTC could potentiate the antitumor effects and reduce drug toxicity will be investigated in our future studies.
In summary, curcumin suppressed the cell viability, migration, invasion and EMT of TPC-1 cells. Moreover, curcumin treatment increased miR-301a-3p expression and inhibited STAT3 expression. Overexpression of miR-301a-3p inhibited cell viability, migration, invasion, and EMT and the JAK/STAT signaling pathway by targeting STAT3, and miR-301a-3p inhibitors and STAT3 overexpression reversed the curcumin-induced cell viability, migration, invasion and EMT of TPC-1 cells. Collectively, curcumin played an anticancer role in TPC-1 cells by regulating miR-301a-3p/STAT3, indicating that curcumin is a promising oncotherapeutic agent. These findings may provide a possible strategy for the clinical treatment of PTC.