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[In vivo ICG fluorescence photometry for quantification of warm ischemia damage of to the liver in a rat model].

The clearance of indocyanine green (ICG) from the peripheral blood has been used for the study of hepatic function in clinical and experimental investigations. We describe a method of simultaneous, semiquantitative measurement of the kinetics of ICG in the peripheral blood, liver, kidney and bile system following intraoperative bolus injection. The method is based on the fluorescence of ICG in the near infrared spectrum. Using this method, we have recognized a temporary extrahepatic storage of ICG within the kidney. Our first results prove changes in the ICG-metabolism fifteen minutes after reperfusion following a 30-minute hepatic ischemia and partial liver resection in the rat.