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Antigenic composition of human renal vascular endothelium assessed by kidney perfusion.

Intravascular perfusion of healthy, viable human kidneys either with human sera or with monoclonal antibodies specific for individual HLA-A, B, DR or E-M antigens demonstrated that all of these antigens are exposed to circulating antibodies and thus can serve as stimuli or targets for immunologic mediators of renal transplant rejection. In addition, these antibodies could be recovered from the renal vessels by brief treatment with acid buffer.