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Notizen: Nanoencapsulated Fluorescence Indicator Molecules Measuring pH and pO2 Down to Submicroscopical Regions on the Basis of the Optode-Principle

To measure pO2 in gases or fluids and pH in solutions the fluorescence indicators pyrene butyric acid and β-methylumbelliferone, respectively, were nanoencapsulated to obtain nano-probes for measurement in physiological structures of nano-range. For pH the fluorescence changes of β-methylumbelliferone were monitored, for pO2 the fluorescence quenching of pyrene butyric acid by oxygen were registered. Drawing log(S1/S2) versus pH, one obtains a straight line between the pH from 6 to 8. A linear increase in pO2 between O and 400 torr yields a linear increase in the reciprocal fluorescence signal.