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[Ophthalmological application of HSV-monoclonal antibodies].

Monoclonal antibodies against HSV (HSV-McAb) were used in the diagnosis of 127 cases of keratitis by immunofluorescence assay (IFA). The positive rate in 43 cases of epithelial and 50 cases of stromal HSK were 100% and 92% respectively. The IFA was negative in 20 cases of keratitis of non-HSV etiology and 38 cases of normal cornea (31 cases of epithelial smears and 7 cases of cornea paraffin sections). Among 14 cases of clinically doubtful HSK, 4 cases were positive. The IFA was completed within 2-3 hours after sampling. The procedure proved rapid, sensitive, and highly specific for etiological diagnosis of HSK.