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Regulation of ornithine decarboxylase in isolated granulosa cells in vitro by constituents of follicular fluid.

The modulation of granulosa cell function by follicular fluid constituents is incompletely characterized. Therefore, we examined follicular fluid pooled from small follicles (FF8) of sexually immature pigs for regulatory properties utilizing hormonally responsive porcine granulosa cells under serum-free conditions in vitro. FF8 contains heat-labile ornithine decarboxylase (ODC)-stimulating factors equipotent with FSH, LH, or prostaglandin E2 (PGE2). Significant activity is preserved after extensive dialysis or 99.5% adsorption of steroids by charcoal. ODC stimulation by submaximal concentrations of FFS is additive to that of saturating doses of LH, FSH, PGE2, or epidermal growth factor. Maximal concentrations of FF8 exhibit synergism with saturating levels of FSH, LH, or PGE2 in ODC stimulation. Preliminary molecular weight sizing of the FF8-stimulating activity by membrane ultrafiltration revealed two active fractions with discrete, saturable, concentration-dependent responses: a high molecular weight su...