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Viremia and virus measurements of rabbit pox in CV-1 cells.

Rabbit pox virus (RPV) produced cytopathic effect (CPE) in five types of cells grown in tissue cultures. The CPE on CV-1 cells was characterized by cell fusion and lysis. The CV-1 line is a useful and sensitive cell culture for measuring concentrations of RPV in blood and tissues of infected rabbits. Viremia was detected between the 2nd and 4th days after parenteral infection. By the 6th and 7th days, the concentration of RPV in various tissues ranged from 10(5-3) to 10(8-5) TCID(50)/g. Cross-reactivity was demonstrated by the fluorescent-antibody technique between rabbit pox, vaccinia, and monkey pox viruses.