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Reduced replication efficacy of SARS-CoV-2 Omicron variant in “mini-gut” organoids

This assay was performed using a cell-based split luciferase system in which donor cells (human embryonic kidney HEK293 cells expressing SARS-CoV-2 spike and HiBiT) and acceptor cells (HEK293 cells expressing ACE2 and LgBiT) were co-cultured. After 24 h, the luciferase activity associated with fusogenicity was measured. In the experiment with REGN-CoV2, we added antibodies to the cells 24 h before measurement.