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[The effects of combined use of multiple growth factors on proliferation and differentiation of human osteoblast-like cells].

Four growth factors PDGF, bFGF, IGF-II and TGF-beta, which is believed to have biological effects on bone cells, were examined in this study. The aim was to assess the effects of combinations of three and four growth factors on the proliferation and differentiation of osteoblast-like cells. The incorporation of 3H-TdR, 3H-Proline of osteoblast-like cells and alkaline phosphatase content of the cells were tested. The results showed the combinations of three and four growth factors stimulated the synthesis of DNA, collagen and ALP of osteoblast-like cells. These four growth factors interacted synergistically. The combinations of three and four growth factors showed stronger promoting effect on osteoblast-like cells, compared with the combinations of two growth factors. These findings suggest that the combined use of growth factors be a potential way of bone defect reconstruction and treatment of human bone disease.