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RNF20 regulates adipocyte differentiation through modulating PPARγ

During adipogenesis, numerous adipogenic transcription factors need to be sequentially expressed and tightly modulated. Among these transcription factors, peroxisome proliferator‐activated receptor gamma (PPARγ) is the most important one during adipogenesis. PPARγ is a nuclear receptor and its activity is sophisticatedly regulated through the binding of coactivators and corepressors. However, the activation mechanisms of PPARγ during adipogenesis are poorly understood. In this study, we demonstrate that the E3 ubiquitin ligase ring finger protein 20 (RNF20) would be involved in adipocyte differentiation by regulating PPARγ. In RNF20 heterozygous‐null mice, the size of adipose tissue and adipocytes were reduced. Knockdown of RNF20 suppressed adipocyte differentiation of 3T3‐L1 cells. Conversely, overexpression of RNF20 promoted adipogenesis. We observed that RNF20 bound to nuclear corepressor (NCoR1) and increased the activity of PPARγ by repressing NCoR1. Taken together, these results suggest that RNF20 would act as a positive regulator for adipocyte differentiation by enhancing the activity of PPARγ.