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Interaction between lung surfactant and nitric oxide production by alveolar macrophages stimulated by group B streptococci

The major etiologic agent in neonatal pneumonia and meningitis is group B streptococci (GBS). Nitric oxide (NO) production by alveolar macrophages (AM) in response to Gram‐positive bacteria such as GBS and the effect of surfactant on this production have received little attention. We studied production of NO by GBS‐stimulated AM using the Griess reaction, the effect of lung surfactant on this NO production, and the possible lipid peroxidation (LPO) of surfactant caused by NO. The LPO test was used to measure surfactant peroxidation.