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Immobilization of Glucose Oxidase on Gel Composites Based on Hydroxyethyl Cellulose and Tetramethoxy-silicane Precursor

Hydroxyethyl cellulose/silicon dioxide gel composites(HEC/SiO 2) prepared by a sol gel method through the hydrolysis and polycondensation of tetramethoxy silicane in a hydroxyethyl cellulose aqueous solution. The gel composites are used as carriers for immobilization of glucose oxidase(GOD). The effects of enzyme content given, phosphate buffer pH, and immobilizing temperature on the activity of immobilized GOD are discussed. The optimum conditions obtained as follows:enzyme content given 21 mg, phosphate buffer pH=6 5, immobilizing temperature 32 ℃. Under the optimum conditions, the bioactivity of the immobilized GOD was as high as 1 000 μmol/(min·g) (dried carrier), about 50 percentage of that of free GOD. The basic proporties of the immobilized GOD are also studied. The results showed that the optimum pH of resultant immobilized GOD and temperature for immobilization were 5 2 and 32 ℃, respectively. The Michaelis constant( K m) is 7 94 mmol/L .