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Fluorescent Specimen Preparation Techniques for Confocal Microscopy

Every good microscopist knows that specimen preparation is critical for obtaining good images. Unfortunately some people think that a confocal microscope can make poorly stained specimens look good. A confocal makes images sharper by rejecting out-of-focus light but it cannot discriminate between specific and non-specific staining or between a specific probe and an autofluorescent background. It takes a picture of all the light coming from a selected focal plane. If anything, a confocal will render artifacts sharper and more beautiful. Therefore, good staining protocols and adequate controls are more important than ever.