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Calcium metabolism related markers during the growth of Haliotis tuberculata

Summary In order to search for a physiological role of calcitonin gene-related peptide (CGRP) during the growth of abalone, the presence of CGRP-like molecules in haemolymph and mantle extracts was investigated. In addition, the activities of carbonic anhydrase and alkaline phosphatase, two enzymes implicated in calcium metabolism of vertebrates, were quantified. The CGRP circulating concentration increased progressively from the 3- to 7-cm-long abalone, whereas the mantle ir-CGRP strongly decreased in 5-cm-long abalone. Moreover, branchial carbonic anhydrase activity, a specific osteoclastic marker in vertebrates, increased during abalone development from 11.8 to 39 WA Units per mg of gill membrane proteins in 4- and 9-cm-long animals, respectively. Mantle alkaline phosphatase activity, a vertebrate specific osteoblastic marker, showed a twofold increase in 6-cm-long animals (8.11 Units per mg of proteins). These studies suggest that CGRP may be involved in molluscan growth, and that gill carbonic anhydrase and the mantle alkaline phosphatase undergo important variations, in relation to the sexual maturation of abalone.