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Elemental Tomography of Biological Structures

Advances in energy-filtered electron microscopy (EFTEM) including the availability of chargecouple device (CCD) detectors with high efficiency and algorithms that can correct for specimen drift make it reasonable to consider the feasibility of elemental tomography. In such experiments, maps of an element are recorded in a tilt series and then the three-dimensional distribution of that element is reconstructed within the specimen volume [1-3]. Recently, this approach has been applied to determine the three-dimensional elemental composition of inorganic materials [4-6]. Here we investigate the feasibility of performing elemental tomography of biological structures.