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74 A SIMPLE METHOD FOR THE DETERMINATION OF BILE ACID POOL COMPOSITION IN MAN

A method has been developed for easy determination of duodenal bile acids. For this purpose Entero-Test(R)was used, an encapsulated nylon thread originally used for diagnosis of enteral parasites. The capsule is swallowed in a fasting state and one end of the thread is taped at a corner of the mouth. Four hours after swallowing the thread, it is withdrawn and bile acids are eluted with phosphate buffer. The solution is applied to a Sep-Pak C18 cartridge to extract bile acids, which are subsequently analysed by capillary gas chromatography and liquid chromatography.In vitro Analyses showed that there was no preferential binding to the thread of any bile acid and that binding was pH independent. A high correlation (r=0.98) was found between direct analyses of bile and analyses by Entero-Test after in vitro incubation. The values obtained by the Entero-Test were similar to those of duodenal bile simultaneously collected with the normal intubation technique (r=0.99). Duodenal bile acid composition showed a variation measured over weeks. In 11 healthy volunteers the following bile acid composition of unstimulated duodenal juice was found (mean ± SD;%) cholate: 44±12 (glycine/taurine ratio 1.8), chenodeoxycholate: 29±6 (G/T ratio 2.3), deoxycholate: 25±11 (G/T ratio 5.7), lithocholate: 1,urnodeoxycholate: < 1.The described technique enables longitudinal studies concerning the effects of nutrients on the bile acid pool composition and its relevance to various diseases.