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Fast Kinetic Analysis of Ligand-Gated Ion Channels

Ligand-gated ion channels mediate fast synaptic transmission in the central and peripheral nervous system and the neuromuscular junction. Their common principle of function is the conversion of a chemical signal—neurotransmitter binding—into an electrical signal, i.e., an ion influx into the postsynaptic cell. The transient nature of this signal requires experimental setups that provide adequate temporal resolution and the use of transient kinetic analysis rather than equilibrium methods for a correct description of receptor function. Although the highly specialized geometry of a synapse that allows very rapid delivery of neurotransmitter is difficult to mimic in an experimental system, a variety of techniques for rapid kinetic analysis are available, making it possible to determine at least some steps of receptor function with sufficient accuracy. This article provides an overview of strategies and methods of fast ligand application and kinetic analysis using whole-cell and single channel patch clamp.