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Simultaneous determination of cotinine and creatinine by high performance liquid chromatography.

Recently, much attention has been focussed on tobacco smoking as a factor harmful to health. Environmental tobacco smoking (ETS) has also been studied for the same reason.1,2) Thus, measurement of the exposure dose of tobacco smoke has become indispensable for the evalua tion of the effects of tobacco smoking and ETS. Cotinine (Cot), a major nicotine metabolite, has recently been examined as a marker of tobacco smoke or ETS because of its long half life and chemical stability.2) Urine creatinine (Cr) is used to adjust the values of urinary biological indicators. Urine Cot and Cr are analyzed separately by different procedures. However, by using ion pair reversed phase chromatography, Cot and Cr can be ana lyzed simultaneously. The urine concentration difference between Cot (ng/ml) and Cr (mg/ml) can be resolved by our original procedure described in "Materials and methods." Using the above mentioned procedure, we de veloped a simplified, speedy and high recovery analytical method for measuring urine Cot and Cr. Materials and methods. Reagents: Cot was obtained from SIGMA, St. Louis, USA, Cr from WAKO PURE CHEMICAL, Osaka, Japan, and sodium 1-decane-sulfonate (for ion-pair chromatog raphy) from TOKYO KASEI, Tokyo, Japan. High performance liquid chromatography: The analyses were performed on TOSOH-HPLC com