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Suitability of Mixing Fluorescent Dye in Adulticides and its Impact on Droplet Characteristics and Pesticide Efficacy1

Fluorescent dyes are commonly used to help visualize insecticidal droplets or to trace movement of insecticides; however, the effect these dyes have on the insecticide’s efficacy and droplet characteristics is unknown. This study evaluated the effects of mixing Uvitex OB fluorescent dye with 5 adulticides on their efficacy in a wind tunnel. Efficacy was determined via droplet size characteristics, spray flux (active ingredient [AI] deposition), and female adult Aedes aegypti mortality. FyfanonH ULV, AnvilH 10+10, Duet, AqualuerH 20-20, and ZenivexH E20, diluted with corn oil, were tested with and without the dye at maximum, minimum, and half-minimum label rates. Adulticide droplet size was not affected by the addition of dye to any of the 5 pesticides tested. Mosquito mortality was strongly correlated with AI deposition for all pesticides except Duet. There was no difference among correlation coefficients of the 5 pesticides and between coefficients of any pesticide pairs, indicating that all correlations were similar. The addition of dye slightly but nonsignificantly and nonconsistently affected mortality. It was found that the source of this variability was due to large variation in mortality among different replicates of the same treatment.