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Determination of Volume of Dunaliella Cells by Lithium Dilution Measurements and Derivation of Internal Solute Concentrations

A method has been developed to measure the cell volume of the unicellular green alga Dunaliella parva 19/9 using Li + measurements only. Concentrations of internal solutes can also be calculated if they are assayed in the same samples as Li + . We found that D. parva cells grown in 0-4 kmol m~3 NaCl have an average aqueous cell volume of 65-1 +2-9 /nm3, a K + concentration of 126 + 6 mol m-3, a Na+ concentration of 9+ 11 mol m~3 and a glycerol concentration of 615 + 27 mol m-3 (n= 12). Algae grown in 1-5 kmol m~3 NaCl have an average aqueous cell volume of 131+7-5 ??m3, a K+ concentration of 109 + 4 mol m-3, a Na+ concentration of 10 + 39 mol m""3 and a glycerol concentration of 1 425 + 59 mol m-3 (n= 12). These results indicate that D. parva cells adapted to high salinities have larger cell volumes than those adapted to lower salinities. However, there is no evidence for a significant difference in internal Na+ concentration, despite the almost 4-fold difference in the concentration of external NaCl. The intracellular glycerol concentration alone accounts for 65% and 54%, respectively, of the osmotic balance in low and high salt grown cells.