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[Comparative study of in vivo and in vitro measurement of reaginic antibody in nasal allergy (author's transl)].

P-K (Prausnitz-Kustner) reaction tests have conventionally been used for proof of reaginic antibodies in vivo in immediate type nasal allergosis. Recently, however, Ishizaka et al.discovered IgE, a carrier of reagin, and Krotzer et al., and Honda et al. made it possible to prove the reaginic antibody in vitro by applying rat mast cell degranulation in allergen-reaginic antibody reactions. As screening of patients with nasal allergosis, measurement of serum IgE levels and an improved type of rat mast cell degranulation test were performed as well as conventional in vivo tests. The results were as follows; 1. In immediate type nasal allergy (Japanese cedar pollinosis and house dust hypersensitivity) the results of the provocation tests and intracutaneous skin tests showed a correlation with the results of the RMCD (Rat Mast Cell Degranulation) method and the appearance rate of eosinophile in nasal secretion. 2. Among Patients tested those having the serum IgE value of 700 ng/ml or more showed positive in the RMCD test, while 80-86% of the patients with the serum IgE value of less than 700 ng/ml also showed positive in the RMCD test. 3. It has been proved that the RMCD method is effective in screening of various sensitized antigens and that this method can be a substitute for the conventional P-K reaction test.