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Platelet HLA Typing and Cross‐Matching by a Direct Microcytotoxic Assay

Abstract: Carboxyfluoresceindiacetate‐labelled platelets can be used in direct microcytotoxic assays for the purpose of typing platelets for a number of antigenic determinants, such as HLA and β‐2 microglobulin. The assay when used in direct platelet cross‐matching, where thrombocytopenic patient sera were screened, resulted in the uncovering of many extra‐positive and weakly positive cross‐matches not identified by the lymphocyte cross‐match. The simplicity and rapidity of this new method makes it an attractive alternative for future use in research and clinical studies.