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[Influence of antimicrobial peptides of human thrombocytes on Staphylococcus aureus biofilm formation].

AIM
Study the influence ofplatelet low molecular weight protein on S. aureus biofilm formation.


MATERIALS AND METHODS
20 clinical isolates of coagulase-positive staphylococci (S. aureus) served as material. Preparation containing a mixture of antimicrobial peptides (fractions with molecular weight 60-70 kDa, 20-25 kDa and 5-10 kDa) obtained from human thrombocyte donors was used. Bio film formation (BFF) by staphylococci was studied photometrically, the degree of hydrophobicity was evaluated by separation of cell suspension in two-phase system liquid-liquid. The data obtained were treated by nonparametric method using Mann Whitney criteria.


RESULTS
Platelet low molecular weight proteins (PLB) at concentration of 2 microg/ml reduced biofilm formation of S. aureus by 48.7 +/- 9.8% from initial values, increased hydrophobicity of plankton and biofilm cell fractions. Maximum inhibitory effect of the preparation containing platelet low molecular weight proteins was noted at concentration of 50 microg/ml.


CONCLUSION
The data obtained on inhibition of staphylococci BFF by platelet low molecular weight protein open a perspective of further studies of PLB as a preparation suitable for control ofbiofilm of persistent microorganisms.