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Three‐dimensional analysis of fibrillar centers and associated chromatin in the nucleolus of human oocytes in primordial follicles

The intranucleolar localization of fibrillar centers and their relationships with nucleolus‐associated chromatin were determined in stereopairs of human oocyte nucleoli obtained by computer reconstruction of serial sections. This study showed that there was no numerical relationship between the number of fibrillar centers and the number of chromosomal NORs. The three‐dimensional reconstruction demonstrated that the majority of fibrillar centers was directly connected with the nucleolus‐associated chromatin.