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Hard to kill: Inactivation of Plasmodiophora brassicae resting spores using chemical disinfectants.

Biosafety practices, such as bioexclusion via sanitization, can prevent the spread of infectious soilborne threats such as the clubroot pathogen, Plasmodiophora brassicae. Twenty three chemical disinfectants were evaluated for efficacy against P. brassicae resting spores. Evans blue staining was used to directly measure the viability of P. brassicae resting spores after 20 min exposures to ten concentrations of each of the 23 chemical disinfectants. Only nine disinfectants were capable of greater than 95% inactivation, and only five were capable of inactivating >99% of resting spores. Bleach (sodium hypochlorite) and Spray Nine® were the most effective disinfectants for inactivation of clubroot resting spores. AES 2500, SaniDate® and ethanol also inactivated >99% of resting spores, but only at very high concentrations. A time course experiment showed that 10 to 12 min contact time was sufficient for ≥ 95% resting spore inactivation with Spray Nine® and sodium hypochlorite, but ≥ 30 min contact was required for other disinfectants evaluated. These results will assist in guiding management recommendations for sanitization aimed at bioexclusion and biocontainment of P. brassicae.