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Fatty Acids from the leaves of Clerodendrum volubile Suppresses Tumor Cell Migration and Invasion, Attenuates Oxidative Stress, and Regulates Cell Cycle Progression in Human Neuronal Glioblastoma Cells

Malignant gliomas have been shown to represent some of the greatest challenges in the management of cancer patients. In this study, the antiproliferative effect of the fatty acid components of Clerodendrum volubile leaves as well as its antioxidant effect on U‐87 MG (Human Neuronal Glioblastoma) cell lines was investigated. Fatty acids extracted from C. volubile leaf oil were subjected to GCMS analysis. U‐87 MG cells were cultured and treated with the fatty acids for 48 hrs, after which the antiproliferative effect was ascertained via MTT assay and cell viability analysis using BD FACS Calibur. Cell cycle was analyzed by flow cytometry on FACS Calibur. Western blotting was used in determining expression of proteins in the cell lines. Cell migration and invasion were analyzed using the migration and invasion transwell assay protocol. The treated cell lines were assessed for reduced glutathione (GSH) level, catalase, superoxide dismutase (SOD) and lipid peroxidation. The fatty acids significantly inhibited cell proliferation, arrested S phase, modulated expression of MMP – 9 and VEGF, attenuated oxidative stress, and suppressed migration and invasion in U‐87 MG cell lines. These results indicate the therapeutic potential of the fatty acids components of the leaves of Clerodendrum volubile on human neuronal glioblastoma cells.