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LPLRFamide causes anorectic effects associated with the brain stem in rats

We investigated the effects of LPLRFamide on appetite‐associated processes using Sprague Dawley rats. LPLRFamide is a member of the RFamide family, a group of neurotransmitters that both stimulate and reduce food intake across many species. We previously showed that intracerebroventricular injection of LPLRF caused decreased feeding in chicks and we hypothesized that this would also be the case in rats. Rats were fasted for 24 hours and those injected with 7 and 15 nmol LPLRFamide, through indwelling guide cannulas aimed at the left lateral ventrical, significantly decreased feeding up to 40 minutes post injection. To investigate the central mechanisms of this effect, 24‐hour fasted rats were injected with 15 nmol LPLRFamide and 30 minutes following treatment, the number of c‐Fos immunoreactive cells was quantified in hypothalamic areas including the dorsomedial, ventromedial, and paraventricular nuclei, as well as the lateral hypothalamic area, and the dorsal motor nucleus (10N) and nucleus of the solitary tract (NTS) of the brainstem. There were a higher number of immunoreactive cells in the NTS and the 10N. These results may be interpreted as LPLRFamide causes anorectic effects in rats that are mediated by the brainstem. These results may aid in the development of anti‐eating treatments.