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Holographic tracking and imaging of free-swimming Tetraselmis by off-axis holographic microscopy.

Digital holography in microscopy plays an important role in optical imaging technique thanks to its noninvasive, label free, quantitative and high-resolution approach. In particular DH allows to obtain the imaging of 3D volume by using the whole complex field of the scattered radiation by the sample by means of numerical reconstruction methods. Thanks to the refocusing feature of digital holography here we show the investigation on the motility of a marine microorganism, the Tetraselmis. We show that this particular type of a marine microalga, travel along complex swimming trajectories that have been retrieved and reconstructed in 3D by means of segmentation algorithms. Motility could be in future correlated to some environmental marine water concerns.