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Detection and Structure Prediction of Polymorphism at Skeletal Muscle-specific Pri-miRNA-133 among Different Sheep Breeds

To study the polymorphism of skeleton muscle-specific pri-miR-133 among different sheep breeds and analyze its correlation with the muscle phenotype,pri-miR-133 polymorphism among four breeds with different meat production performance(Texel,Chinese Merino Fine Wool,Altay and Hu Sheep),and the effects of the pri-miR-133 bpolymorphism on its secondary structure,stability and the capacity of mature miR-133 processing were detected and analyzed by PCR-SSCP.The result showed that there was a A/G transversion at 194 bp downstream of pre-miR-133 b,and the SNP was clearly associated with its corresponding meat traits.In Texel flock,GG genotype was the preponderant genotype,and G allele frequency reached 0.86,the value of G allele frequency in Texel was four times the frequency of G allele in Chinese Merino flock.The Secondary structure further bio informatics analysis showed that the mutation significantly changed the secondary structure of pre-miR-133,and the free energy value was reduced by 3.2KJ/mol.The above indicated that the SNP in pri-miR-133 may have a significant impact on the processing of mature miR-133,and affect the performance of sheep meat production.