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Osmotic erythrocyte lysis for chemical- and label-free impedance cytometry

We present the first implementation of osmotic erythrocyte (RBC) lysis for microfluidic leukocyte (WBC) impedance cytometry. The method relies on osmotic pressure differentials to rupture RBC membranes, and detection of WBCs based on changes in impedance. Specifically, we demonstrate an up to 1,200-fold decrease in noise from RBCs in WBC recordings. Our approach enables lab-on-a-chip (LOC)-based WBC counting on whole blood samples without traditionally required chemicals or labels.