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Isolation of globin messenger RNA by preparative polyacrylamide gel electrophoresis.

Abstract Preparative scale polyacrylamide gel electrophoresis has been employed for isolating milligram quantities of globin messenger RNA from total RNA of rabbit reticulocyte ribosomes. Adherence to defined conditions for gel formulation, polymerization and electrophoresis have allowed recovery of globin messenger RNA within 24 hours electrophoresis time. The biologic activity of mRNA so isolated has been determined by assay in mRNA-dependent protein synthesis systems, and the products identified by standard procedures as rabbit globin. Application of preparative scale polyacrylamide gel electrophoresis for isolating other mRNAs as well as for resolving various similar molecular weight messenger RNAs (i. e., alpha-and beta-globin messages) is concluded to be feasible and is being investigated.