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Absence of fractionation, protraction, radiation quality, and radical scavenger effects on radiation-induced interphase death of human G0 lymphocytes in vitro.

In vitro interphase death of human peripheral blood lymphocytes, measured by shortening of the mean lifetime of small G0 lymphocytes, was studied with 150 kV and 10 kV X-rays. No effect of radiation quality, of fractionation and of protraction was observed. The possibility of protection by L-cysteine and DMSO was tested in cells irradiated with 150 kV X-rays, but no protection effect with respect to cell survival could be demonstrated. Non-DNA radiation-sensitive site(s), possibly the cell membrane, appear to be responsible for interphase death.