{'fraction_non_alphanumeric': 0.044466173115425806, 'fraction_numerical': 0.04996042277785538, 'mean_word_length': 4.752008569898233, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Novel IL-12 family members shed light on the orchestration of Th1 responses

Interleukin-12 (IL-12), which is composed of a p35 and a p40 subunit, is a proinflammatory natural-killer (NK) cell-stimulating, Th1-inducing and Th1-maintaining cytokine, which promotes cell-mediated immunity. On activation, heterodimeric IL-12 is found in small amounts, whereas free p40 is produced in excess. Besides IL-12, other p40-dependent molecules exist that orchestrate Th1 responses. Homodimeric p40 can act as an IL-12 antagonist by competing for its receptor. Recent data also reveal potential immunostimulatory functions of p40. In addition, p40 can be covalently linked to a p35-related protein p19. This heterodimer is known as IL-23 and has activities on memory T cells. Finally, IL-27, the latest addition to this family, is a heterodimer composed of the p40-related protein EBI3 (Epstein–Barr virus-induced gene 3) and the p35-related protein p28. IL-27 is involved in early Th1 initiation.

Interleukin-12 (IL-12), which is composed of a p35 and a p40 subunit, is a proinflammatory natural-killer (NK) cell-stimulating, Th1-inducing and Th1-maintaining cytokine, which promotes cell-mediated immunity. On activation, heterodimeric IL-12 is found in small amounts, whereas free p40 is produced in excess. Besides IL-12, other p40-dependent molecules exist that orchestrate Th1 responses. Homodimeric p40 can act as an IL-12 antagonist by competing for its receptor. Recent data also reveal potential immunostimulatory functions of p40. In addition, p40 can be covalently linked to a p35-related protein p19. This heterodimer is known as IL-23 and has activities on memory T cells. Finally, IL-27, the latest addition to this family, is a heterodimer composed of the p40-related protein EBI3 (Epstein -Barr virus-induced gene 3) and the p35-related protein p28. IL-27 is involved in early Th1 initiation.
With the discovery of interleukin-12 (IL-12) as a heterodimeric cytokine composed of a p35 and a p40 subunit, free p40 (mostly monomeric and homodimeric) was also found and characterized. About 10 years later a novel p40p19 heterodimeric molecule (IL-23) was described, and this was shortly after followed by a p40-related molecule dimerized with a p28 protein (IL-27). These novel molecules not only establish the IL-12 family but also help to reveal distinct cellular and functional stages of Th1 development.
IL-12 -the prototypic Th1-inducing and Th1-maintaining molecule IL-12 (also termed IL-12p75 or IL-12p70 but commonly designated IL-12) is an immunoregulatory cytokine that promotes cell-mediated immunity [1,2]. IL-12 stimulates production of interferon-g (IFNg) from T cells and naturalkiller (NK) cells. Studies in IL-12-deficient mice have demonstrated an essential role for IL-12 in the induction of Th1 responses, which are especially required for protection against intracellular microorganisms [3,4]. IL-12 is not only involved in induction but also in maintenance of Th1 responses [5,6]. Among the cytokine family, IL-12 has an atypical heterodimeric structure. It is composed of a p35 and a p40 subunit (Table 1), each of which is expressed by its own gene and on different chromosomes. The p35 gene has homology with other class I cytokines, especially IL-6 and granulocyte-colony stimulating factor (GCSF) [7]. Interestingly, the p40 subunit is homologous to the extracellular domain of the hemopoietic cytokine receptor family, in particular to the extracellular domains of the IL-6 receptor a-chain (IL-6Ra) and the ciliary neurotrophic factor receptor (CNTFR) [8]. Coexpression of both chains of IL-12 in one cell is required to generate bioactive IL-12 [9]. Although p35 transcripts are found in many cell types, free p35 is not secreted [10]. Production of IL-12 by activated macrophages and dendritic cells (DCs) results in secretion of a 10 -1000-fold excess of free monomeric and homodimeric p40 relative to heterodimeric IL-12 [10]. The activities of IL-12 are mediated by a high-affinity receptor composed of two subunits, designated b1 and b2 [1] ( Table 1). Both b1 and b2 are members of the class I cytokine receptor family and are most closely related to glycoprotein gp130 and the receptors for leukemiainhibitory factor and GCSF. Both receptor subunits can be expressed on NK and T cells and are required for IL-12 bioactivity [1,11]. The b2 subunit acts as signal transducer by providing a cytoplasmic STAT4 (signal transducer and activator of transcription 4) binding site, which enables STAT4-mediated responses to IL-12 to occur [12,13]. Studies of b1 2/2 and b2 2/2 mice confirmed the essential role for both subunits in mediating the biological functions of IL-12 on NK and T cells [14,15].
p40 -a Th1 regulator with two faces: not only an in vitro and in vivo antagonist of IL-12 but also a potential agonist In vitro, the soluble p40 subunit of IL-12 is able to antagonize IL-12 bioactivity [16]. In vivo, about a third of the free p40 in the serum of endotoxin-challenged mice is present in the homodimeric form, the remainder consists of monomeric p40 [17]. For both murine and human homodimeric p40, (p40) 2 is able to act as antagonist of IL-12 by binding to the b1 subunit of the IL-12 receptor [18,19]. Pretreatment of mice with homodimeric p40 was able to reduce serum IFNg levels and protect mice from lipopolysaccharide (LPS)-induced death [17,20]. The antagonistic potential of (p40) 2 was further confirmed by reduced Th1 responses of transgenic mice in which the p40 gene is regulated by a liver-specific promoter [21]. However, because of its low affinity to the human IL-12R, human (p40) 2 has only a minor ability to antagonize IL-12 functions compared to murine (p40) 2 [22]. The antagonistic potential of (p40) 2 might, therefore, represent a phenomenon specific for the mouse but could be different in humans. Murine homodimeric p40 is 25 -50-fold more potent as an IL-12 antagonist than monomeric p40, as shown in both binding assays and bioassays [18]. In physiological situations the effect of IL-12 appears to be dominant despite the excess of p40. However, reducing the excess of p40 (without changing the levels of IL-12p75) by generating N-glycosylation mutants of p40 led to enhanced cytotoxic T-lymphocyte (CTL) responses and to better protection against tumor challenge [23]. This suggests that excessive levels of p40 physiologically dampen IL-12-dependent bioactivity as a result of its antagonistic properties.
The first evidence of an agonistic activity of homodimeric p40 was shown in vitro. Induction of CD8 þ Th1 differentiation in a mixed lymphocyte culture was enhanced in the presence of (p40) 2 [24]. Moreover, the same group subsequently showed in vivo that endogenous p40 produced by allografted IL-12p35 2/2 mice is able to stimulate alloreactive CD8 þ Th1 development [25]. By contrast, IL-12p40 2/2 mice, which are unable to produce p40, generated less IFNg than IL-12p35 2/2 mice [25]. Treatment of allografted IL-12p35 2/2 mice with a monoclonal anti-IL-12p40 antibody was able to abrogate this IFNg production [25]. Therefore, IL-12p40 is, in addition to its antagonistic activity, able to act in vitro and in vivo as an agonist similar to IL-12. However, in light of the recently discovered p40-composed heterodimer p40p19 (designated IL-23) it cannot be ruled out that heterodimeric IL-23 was also neutralized by the anti-IL-12p40 monoclonal antibody.
Treatment of infected IL-12p35/40 2/2 mice with homodimeric p40 restored the observed defect in antigen-specific delayed-type hypersensitivity (DTH) responses [26]. Thus, endogenous and exogenous p40 induces protective immunity in mycobacterial infection. The reconstitution experiments could not exclude the possibility that p40 is able to associate with extracellular p19 to form bioactive IL-23. However, because p19 is not secreted on its own (as is the case for p35) [10,28], it is rather unlikely that extracellular monomeric p40 is able to form bioactive IL-23.
In addition, in a paramyxoviral bronchitis model, p40-dependent (monomeric and homodimeric p40) epithelial macrophage accumulation and increased mortality was observed [29]. Interestingly, the results from this virally induced murine model of airway inflammation could be extended to patients with asthma, in which airway levels of predominantly homodimeric p40 and airway macrophages were elevated relative to normal subjects [29]. This suggests that the agonistic effect of (p40) 2 as opposed to its antagonistic property is also significant in humans. Together, excessive levels of p40 associated with macrophage accumulation in Sendai-virus infected IL-12p35 2/2 mice and in patients with asthma strongly argue for an agonistic potential of homodimeric p40, which can even result in immunopathological responses. Recently, homodimeric p40 was found to be a chemoattractant for macrophages in vitro and in vivo, supporting the discussed effects during bronchitis and asthma [30]. Also, p40-dependent pulmonary fibrosis and macrophage infiltration was observed in a murine model of silica-induced lung fibrosis [31]. Administration of recombinant (p40) 2 to silica-treated mice resulted in transient lung fibrosis and macrophage influx to the lung [31]. Taken together, endogenous and exogenous p40 are able to induce lung macrophage accumulation and fibrosis.
In vitro, a direct activating effect of homodimeric p40 on macrophages was further supported by Pahan et al. [32], who showed that inducible nitric oxide (iNOS) expression and NFkB activation could be induced by (p40) 2 in mouse primary microglia and peritoneal macrophages but not in mouse primary astrocytes. Interestingly, even the monomeric p40 was found to stimulate NO production provided the monocytic cells were activated by IFNg [32]. Homodimeric p40 binds to IL-12Rb1 but not to IL-12Rb2 [33]. Similar to IL-12, murine (p40) 2 binds with both high and low affinity to IL-12Rb1 on Concanavalin A blasts and B cells [34]. The binding of (p40) 2 to IL-12Rb1 (thereby preventing IL-12 binding) provides a molecular mechanism for the antagonistic effect of (p40) 2 on IL-12. Moreover, it is conceivable that IL-12Rb1 is also responsible for the agonistic effect of (p40) 2 . IL-12Rb1 and IL-12Rb2 associate with different Janus kinases and therefore might contribute to distinct signaling pathways: the cytoplasmic domain of IL-12Rb1 associates with tyrosine kinase 2, and the cytoplasmic domain of IL-12Rb2 interacts with Janus kinase 2 [35]. Hence, IL-12Rb1 might be capable of transducing IL-12p40 signals through tyrosine kinase 2. In addition, a yet to be discovered novel receptor subunit might associate with IL-12Rb1 for (p40) 2 -mediated agonistic activities. A hitherto unidentified third component associating with the IL-12Rb1 subunit was recently reported [36].
IL-23 -the late actor in the Th1 program: a p40 -p19 heterodimer with activity on memory T cells Searching sequence databases with a computationally derived profile of IL-6 subfamily structures, a novel protein p19 was identified that is able to build a disulfide-bridged complex with the p40 subunit originally described for IL-12 [28]. This novel heterodimeric molecule was designated IL-23. The p19 protein is, similar to the p35 protein, biologically inactive by itself [28]. IL-23 requires interaction with IL-12Rb1 [28] and an additional, novel b2-like receptor subunit designated IL-23R with a cytoplasmic STAT4 binding domain [37]. Similar to IL-12, coexpression of p19 and p40 in the same cell appears to be required for secretion of IL-23 [28]. The p40 -p19 complex is secreted by activated murine and human DCs [28]. In the mouse, the biological activities of IL-23 are distinct from IL-12. Murine IL-23 was not found to induce significant amounts of IFNg. Murine IL-23 induces strong proliferation of memory T cells but not of naïve T cells, whereas IL-12 has no effect on memory T cells [28]. In addition, recently it was shown that IL-23 (but not IL-12) can activate murine memory T cells for production of the proinflammatory cytokine IL-17 [38]. In humans, IL-23 has biological activities that are less distinct from IL-12 (proliferation of memory T cells, modest IFN-g production by naïve and memory T cells compared to IL-12) [28]. Transgenic expression of p19 leads to multi-organ inflammation, runting, infertility and premature death [39]. The p19-transgenic mouse shows infiltrates of lymphocytes and macrophages in organs, elevated serum tumor necrosis factor-a (TNF-a) and IL-1 levels, increased numbers of circulating neutrophils and constitutive expression of acute phase proteins in the liver [39]. In the light of these data it is possible that the p40-dependent but p35-independent defect in granuloma formation and DTH responses observed in several infectious disease models might in fact be due to a lack of IL-23 [40,41]. Indeed, reconstitution of Cryptococcus neoformans-infected IL-12p40 2/2 mice with recombinant monomeric or homodimeric IL-12p40 failed to protect these mice from a fatal course [40]. Mice with keratinocyte-specific transgenic expression of p40 developed an inflammatory skin disease phenotype [42] reminiscent of the p19-transgenic mice [39]. Interestingly, the inflammatory response following transgenic p40 expression could not be mimicked by injection of homodimeric p40 into the skin of littermate mice [42]. This argues for a molecule different from homodimeric p40, for example, IL-23, responsible for the observed eczematous skin disease. Studies in recently generated p19-deficient mice might clarify these questions.
IL-27 (EBI3-p28) -the early riser in Th1 responses, which was found latest: a p40 relative involved in Th1 initiation Recently, a further member of the IL-12 family was described and termed IL-27 [43]. IL-27 is a heterodimeric protein that consists of Epstein -Barr virus (EBV)-induced gene 3 (EBI3), a p40-related protein, and p28, a newly discovered IL-12p35-related polypeptide. IL-27 appears to be produced early by activated antigen-presenting cells. It is able to induce clonal proliferation of naïve but not memory CD4 þ T cells and synergizes with IL-12 in IFNg production by naïve CD4 þ T cells [43]. Recently, an orphan receptor was described with 26% homology and 37% similarity to the IL-12Rb2 subunit and to gp130, designated TCCR [44] or WSX-1 [45]. This receptor is essential for early initiation of Th1 responses but neither binds IL-12 nor associates with the IL-12R subunits [43,44]. Instead, this receptor was identified as one of the receptor subunits for IL-27 and as necessary but not sufficient for IL-27 function [43]. Interestingly, whereas activation of TCCR or WSX1 is required for the early initiation of a Th1 response, it is not necessary for the maintenance of Th1 responses [5,6,45]. It is possible that IL-27 and IL-12 function sequentially in initiating and maintaining Th1 responses, respectively [43,45]. Such a view is supported by high TCCR mRNA expression on undifferentiated Th cells but low expression on differentiated Th1 and Th2 cells enabling IL-27 to activate Th0 cells [44]. However, on naïve Th cells IL-12Rb2 is upregulated on antigen activation and during IL-12-driven Th1 development [46,47]. This suggests that IL-27 can act before IL-12.
EBI3 was initially identified in B lymphocytes infected with EBV [48]. EBI3 is related to p40 showing 27% homology at the amino-acid sequence level [48]. Importantly, EBI3 is expressed in monocytes and macrophages, as is IL-12. Thus, pokeweed-mitogen activated peripheralblood mononuclear cells express EBI3 [48]. Human monocytes activated for IL-12 production also induced EBI3 gene transcription, although with slower and more prolonged kinetics than for IL-12 [2]. Interestingly, EBI3 is expressed throughout human pregnancy by cells of fetal origin [49] and some of the EBI3 expressed in placental trophoblasts was found to be associated with the p35 subunit of IL-12 [50]. Expression of EBI3 in EBV-infected cells as well as during pregnancy led to the suggestion that free EBI3 and/or EBI3 -p35 might counter-regulate type 1 responses [48] similar to the way by which (p40) 2 was found to antagonize the immunostimulatory action of IL-12. However, the function of EBI3 as a potential downregulator of IL-27 action remains speculative. Recently, it Review TRENDS in Immunology Vol. 24 No.4 April 2003 was shown in EBI3 2/2 mice that EBI3 or an EBI3-dependent homo-or hetero-dimeric factor is essential for growth and differentiation of IL-4 producing invariant NKT cells [51]. EBI3 2/2 mice exhibited a reduced number of invariant NKT cells, a sustained decrease in IL-4 production and were resistant to Th2-mediated immunopathology associated with oxazolone-induced colitis [51]. Interestingly, IFN-g production was only transiently decreased in EBI3 2/2 mice and EBI3-deficient mice were as susceptible as wild-type mice in a Th1-mediated colitis model induced by trinitrobenzene sulfonic acid [51]. These data suggest that an EBI3-dependent factor different from IL-27 is involved in IL-4-mediated Th2 responses (Fig. 1).
Role of the IL-12 family members in infectious diseases IL-12 has a key role in protection against intracellular protozoan, fungal and bacterial infections [4,5,40,52,53] ( Table 2). The role of IL-12 in protection and pathology during viral infections depends on the type of virus [54 -57]. Interestingly, for several viruses (shown in Table 2) protective type-1 immunity was completely IL-12-independent and also independent of other members of the IL-12 family, pointing to other Th1-inducing factors for immunity against these viruses. This confirms data from patients with mutations in the IL-12p40 or the IL-12Rb1 gene who responded normally to standard viral immunizations but developed chronic courses of salmonellosis or mycobacteriosis [58]. In some intracellular fungal, bacterial and some viral infections other p40dependent and p40-related proteins (different from IL-12), that is, (p40) 2 , IL-23 and IL-27 (and potentially other as yet unknown members of the IL-12 family), are able to contribute to type-1 responses ( Table 2). It is of special interest that many of these infections (especially mycobacterial infections) tend to develop a chronic course by a pathogen with low virulence. Moreover, it is noteworthy that for salmonellosis other p40-dependent proteins different from IL-12 only have a detectable role at low infective doses ( Table 2) [41]. Thus, the function of members of the IL-12 family appears to depend on the type of pathogen as well as on the dose of the pathogen. Low-dose infections can be controlled independently of IL-12 but depend on other members of the IL-12 family in some experimental murine models. By contrast, high-dose  infections require IL-12 for Th1 induction. This hypothesis could be based on a dose-and pathogen-specific regulation of synthesis of p40, p35, p19, EBI3 and p28. In addition, there might be a characteristic time course for synthesis of p40, p35, p19, EBI3 and p28 during infection [43]. Interestingly, microbial components appear to induce primarily p40 and relatively low amounts of IL-12, whereas subsequent cross-linking of CD40 on antigenactivated DCs by CD40L-expressing T cells results in amplification of IL-12 production by increasing p35 expression [59,60]. More recently, IFNb was shown to differentially affect CD40L or IFNg-induced induction of the IL-12 family members (inhibition of p35 and p40, enhancement of p19 and EBI3 transcription) in DCs resulting in inhibition of IL-12 production [61].
Multiple activating and inhibitory members of an emerging cytokine family contribute at different stages to induction and inhibition of type-1 cellular immunity (Fig. 2). According to currently available data the receptor expressed on the target cell has a crucial role. Expression of the receptor for IL-27 on naïve Th cells enables the initiation of Th1 responses [43,44], subsequent upregulation of IL-12Rb2 [46] enables IL-12 to become active in induction and maintenance of effector Th1 responses [5,6]. However, activation of Th1 memory cells appears to be restricted to IL-23 [28]. Moreover, the IL-1-related cytokine IL-18 will synergize with IL-12 as soon as its receptor is induced by IL-12 [62]. The orchestration of Th development ultimately results in regulated effector mechanisms, such as IFNg-dependent macrophage activation, granuloma formation and DTH responses. IL-27 appears to activate Th0 and Th0/1 cells, which express WSX-1 (identical with TCCR). After induction of IL-12Rb2 expression IL-12 is able to drive further differentiation and maintenance of Th1eff cells. Homodimeric p40 is able to antagonize IL-12 at its receptor or to contribute to Th1 activation by interacting with IL-12Rb1 and possibly a putative second receptor component. IL-23 is able to activate Th1mem cells. There is synergy between IL-27 and IL-12, as well as between IL-12 and IL-18. IL-12 induces the receptor for IL-18 on Th cells [66]. The receptors for IL-12 and IL-23 are also expressed on APCs or APC subpopulations (not shown in this figure). Thus, regulation of APC activity might be modulated, as well as T-cell development, by IL-12 and IL-23. Abbreviations: APC, antigen-presenting cell; IL-12, interleukin-12; R, receptor; Th0, naïve T cells; Th0/1, differentiating naïve T cells; Th1eff, effector T; Th1mem, memory Th1.