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Sequence of the gene encoding the mitochondrial F1-ATPase alpha subunit from Nicotiana plumbaginifolia

A genomic bank of Nicotiana plumbaqinifolia mitochondrial DNA was constructed and probed with the gene encoding the a subunit of maize mitochondrial Fn-APTase (1,2). A 4.7 kb insert was present in the positive clones isolated. After subcloning, a series of progressive deletions were generated. Sequence analysis revealed a region of 1527 nucleotides encoding a polypeptide of 55,162 molecular weight. The amino acid sequence shares respectively 94,3 % et 93,9 % homology with the a subunit sequence of maize (1,2) and Oenothera (3).