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DNA content in renal cell carcinoma. A comparison between flow and static cytometric methods.

Different flow and static cytometric techniques for analysis of DNA content were compared using imprints, fresh and paraffin-embedded tissues from renal cell carcinomas. All methods discriminated between diploid-/near diploid and aneuploid samples and gave comparable results concerning modal ploidy levels (r = 0.744-0.970). For retrospective investigations of renal cell carcinoma, flow cytometry using paraffin-embedded tissues seemed to be the method of choice in determination of DNA content.