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PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes

Objective Brown adipocytes play a key role in maintaining body temperature as well as glucose and lipid homeostasis. However, brown adipocytes need to adapt their thermogenic activity and substrate utilization to changes in nutrient availability. Amongst the multiple factors influencing brown adipocyte activity, autophagy is an important regulatory element of thermogenic capacity and activity. Nevertheless, a specific sensing mechanism of extracellular amino acid availability linking autophagy to nutrient availability in brown adipocytes is unknown. Methods To characterize the role of the amino acid transporter PAT2/SLC36A2 in brown adipocytes, loss or gain of function of PAT2 were studied with respect to differentiation, subcellular localization, lysosomal activity and autophagy. Activity of vATPase was evaluated by quenching of EGFP fused to LC3 or FITC-dextran loaded lysosomes in brown adipocytes upon amino acid starvation, whereas the effect of PAT2 on assembly of the vATPase was investigated by Native-PAGE. Results We show that PAT2 translocates from the plasma membrane to the lysosome in response to amino acid withdrawal. Loss or overexpression of PAT2 impair lysosomal acidification and starvation-induced S6K re-phosphorylation, as PAT2 facilitates the assembly of the lysosomal vATPase, by recruitment of the cytoplasmic V1 subunit to the lysosome. Conclusions PAT2 is an important sensor of extracellular amino acids and regulator of lysosomal acidification in brown adipocytes.


INTRODUCTION
Brown adipocytes, with their unique ability to dissipate excessive energy in form of heat through mitochondrial uncoupling, play an important role in regulating energy expenditure, as well as glucose and lipid homeostasis [1e3]. Importantly, brown adipocyte activity itself is tightly regulated by environmental signals, as well as the metabolic state of the organism [4e12]. This underscores the important role of brown adipocytes as rheostat sensing the organismal state to regulate whole body metabolic function through a complex network of neuronal, endocrine and nutritional inputs. The role of the sympathetic nervous system, as well as glucose, fatty acids and other metabolites have been extensively described in the regulation of brown adipocyte activity [4e6, 13,14]. However, surprisingly little is known about the potential role of amino acids in the regulation of brown adipocyte function. Alanine inhibits glucose oxidation of brown adipocytes [15], whereas leucine as well as arginine promote BAT growth and function [16,17]. Cellular amino acid levels are sensed and regulated by a complex network of proteins in and around the lysosome centered around mTORC1 activity [18]. Conditional ablation of raptor, an essential protein for mTORC1 activity, in adipocytes results in increased lipolysis and lipophagy, which is rescued by inhibition of autophagy through depletion of ATG7 [19]. In line with this, autophagy regulates adipocyte differentiation, thermogenic gene expression and brown fat activity [20,21]. Autophagy mediates degradation of various intracellular components through their delivery to the lysosome [22e24]. Thus, the lysosome governs intracellular lipid and amino acid availability and can modulate energy metabolism in brown adipocytes [25,26]. A central element for the proteolytic activity of the lysosome is vATPase-dependent luminal acidification [27]. vATPase activity itself is primarily regulated via its assembly, through the recruitment of the cytosolic V1 subunit to the membrane anchored V0 subunit during the maturation from the late endosome to the lysosome [28]. We previously identified the proton-coupled amino acid transporter PAT2/ SLC36A2 as highly enriched in brown adipocytes [29]. However, in contrast to other brown adipocyte enriched genes, PAT2 expression was not regulated by chronic cold exposure or CL-316243 treatment and only slightly downregulated in BAT of db/db mice compared to db/ þ controls [29]. PAT2 is a proton-coupled amino acid transporter that belongs to the SLC36 family [30,31], with very narrow substrate specificity [32] and strong pH dependence [32e35]. We showed that in contrast to the constitutive lysosomal amino acid transporter PAT1, PAT2 localizes to the plasma membrane of fully differentiated brown adipocytes [29]. However, the function of PAT2 in brown adipocytes is not known. Here we show that PAT2 resides at the cell surface of mature brown adipocytes and responds to changes in extracellular amino acid levels, as depletion of extracellular amino acids results in rapid translocation of PAT2 from the cell surface to the lysosome. We demonstrate that PAT2 interacts with and recruits the V1 domain of the vATPase to the lysosome, facilitating full assembly of the enzyme. Deregulation of PAT2 by either overexpression or knockdown results in hyper-or hypo-acidification of the lysosome, respectively, with profound effects on autolysosome formation and activation of mTORC1. Together, PAT2 serves as an amino acid sensor orchestrating intracellular organelle functions in brown adipocytes.

Cell culture
For all experiments, a previously established murine brown preadipocyte cell line, derived from heterogeneous stromal vascular cells in BAT of an 8 week-old C57BL/6 mouse was used, cultured and differentiated as previously described [48]. To establish a PAT2 knockdown cell line, a shPAT2 (targeting sequence: CCGGCA-GACTGAACAAGCCTTTCATCTCGAGATGAAAGGCTTGTTCAGTCTGTTTT TG) and its scrambled control shRNA (shScr), cloned into a pLKO.1puro vector were purchased from Sigma Aldrich. PAT2 cDNA containing a HA-tag directly in front of the stop codon was cloned into the pCDH-CMV-puro plasmid to generate the PAT2-HA overexpression cell line. All plasmids were packed in lentiviruses (Cell Biolabs, VPK-205), concentrated using PEG-it (System Bioscience, SBI-LV810A-1) and preadipocytes were infected in presence of 9 mg/mL polybrene. Cells were cultured in medium containing DMEM (Thermo Fisher Scientific, 35050038), 10% fetal bovine serum, 1% penicillin-streptomycin (Thermo Fisher Scientific, 5000956) and 2.5 mg/mL puromycin (Cayman Chemical, Cay13884-500).

Transient transfection
Thirty microliter DMEM, 20 mL Polyfect (Qiagen, 301107) and 1 mg plasmid were incubated for 5 min and the transfection mix was dropped to cover all cells without medium. After 4 h incubation, cell culture medium was added.

Mouse experiments
Male C57BL/6N-Rj mice were purchased from Janvier and housed under 45e65% humidity and a 12h light: 12 h dark cycle at ambient temperature 22 AE 2 C. For the fasting experiments, mice were transferred at the beginning of the dark cycle to a new cage without food but ad libitum access to water for 12 h. Animal experiments were conducted in accordance with the German animal welfare law and performed with permission and in accordance with all relevant guidelines and regulations of the district government of Upper Bavaria (Bavaria, Germany), ROB-55.  (Table S1) overnight at 4 C. Ten microliter protein A/G agarose beads (Santa Cruz Biotechnology, sc-2003) were added to the lysate for 1 h at 4 C. The beads were precipitated by centrifugation at 1,000Âg at 4 C for 3 min. Lysis buffer was used to wash beads 3 times and proteins were eluted with 2 Â NuPAGEÔ LDS Sample Buffer with 5% b-mercaptoethanol (Sigma Aldrich, M3148) at 70 C for 5min and analyzed by western blot.

Blue native-PAGE
The NativePAGE Novex Bis-Tris gel system (Thermo Fisher Scientific, BN1003BOX) was used according to the manufacturer's instruction using 1% digitonin and NativePAGE 3e12% Bis-Tris gels. Gels were soaked in 0.1% SDS in TBST for 10 min before transfer to 0.45 mm PVDF membranes using a Bio-Rad wet tank blotting system with 0.01% SDS in Tris-glycine transfer buffer containing 10% methanol. The membrane was incubated in 8% acetic acid in TBST for 15 min and subsequently washed with double distilled water. The remaining Coomassie G250 dye was removed with 100% methanol and the membrane used for western blot.
2.6. Subcellular fractionations Subcellular fractionation was performed as previously described [43]. In brief, cells were homogenized in fractionation buffer (250 mM sucrose, 1 mM EDTA and 10 mM HEPES pH 7.4) containing protease and phosphatase inhibitors using a Potter-Elvehjehm grinder on ice. Homogenates were centrifuged at 500Âg for 10 min at 4 C and the supernatant at 100,000Âg for 30 min at 4 C to result in membrane pellet. The cytosol fraction in the supernatant was concentrated using 10K Polyethersulfone (PES) membranes (VWR, 516-0229) according to the manufacturer's instructions. The membrane pellet was washed with fractionation buffer. The cytosol and membrane fractions were dissolved with 0.1% SDS and applied to analyses by western blot.

Immunofluorescent staining and imaging
Cells were cultured on chamber slides (Thermo Fisher Scientific, 154534 PK), fixed with 4% PFA (Sigma Aldrich, P6148) or methanol for 10 min. Tissues were fixed with 4% PFA for 1 h prior to vibratome (Leica) sectioning at 100 mm-thickness. Cells or tissue sections were washed with PBS and 3% BSA and 0.3% Triton-X 100 in PBS were used for blocking and permeabilization for 1 h. Samples were incubated with primary antibodies for 2 h or overnight and Alexa conjugated secondary antibodies (see Table S1) for 1 h. DAPI (Merck Millipore, 508741) diluted in PBS (1 mg/mL) was added to the cells after the secondary antibody for 5 min. Cells and tissue sections were mounted with mounting medium (Dako, S302380-2) and images acquired using a Leica TCS SP5 confocal microscope. Image quantification and co-localization analysis were performed using ImageJ.
2.7.1. EGFP quenching pBABE-puro mCherry-EGFP-LC3B was obtained from Addgene (#22418, Jayanta Debnath lab) [41]. The plasmid was transiently transfected into adipocytes cultured in live cell imaging chamber slides (ibidi, 80827). Following the amino acid starvation for 1 h, the medium was changed to live cell imaging solution (Thermo Fisher Scientific, A14291DJ) and images were acquired by confocal microscopy maintaining 5% CO 2 and 37 C during imaging. Relative intensities of mCherry and EGFP were quantified by ImageJ software.
2.7.2. Intracellular pH measurements pEGFP-LC3 (human) was obtained from Addgene (# 24920, Toren Finkel lab) [49]. The plasmid was transiently transfected into adipocytes. pHrodo Red AM (Thermo Fisher Scientific, P35372) was used to assess intracellular pH following the manufacturer's instruction and imaged as described above. Images were analyzed for co-localization of red and green pixels, and signal intensity by ImageJ software.

In vitro quenching test
The protocol was modified from the published method [43] as described. Adipocytes were loaded with 2.2 mg/mL FITC-Dextran 70,000 (Sigma Aldrich, 46945) in culture medium overnight. The medium was replaced with culture medium or amino acid free DMEM for 1 h. The adipocytes were homogenized in 125 mM KCl, 1 mM EDTA, 50 mM sucrose, 20 mM HEPES pH 7.4, 1% phosphatase and protease inhibitors cocktail using a Potter-Elvehjehm grinder on ice. Big particles were removed by centrifugation at 2,000Âg for 10 min at 4 C. The FITC-Dextran loaded vesicles were pelleted by centrifugation at 16,100Âg for 15 min at 4 C and the resulting pellet was resuspended in homogenization buffer. Protein concentration was measured using a BCA kit. Particles corresponding to 4e20 mg protein were added with or without 1 mM concanamycin A (Santa Cruz Biotechnology, sc-202111) to flat glass bottom plates to measure FITC fluorescence at 488 nm at 37 C. Fluorescence intensity was recorded every 2 s for 30 cycles and for additional 120 cycles after addition of 1 mM ATP (Serva Electrophoresis, 10920.01) and 2 mM MgCl 2 . All intensities were normalized to the baseline of Scr adipocytes samples cultured in regular culture medium. Data transformation was performed according to the Stern-Volmer equation [50] and slopes subsequently calculated. 2.11. Cellular respirometry Oxygen consumption of brown adipocytes was measured by an XF96 Extracellular Flux Analyzer (Seahorse Bioscience) as described in [40]. Cellular respirometry was performed at day 7e8 days of differentiation. Cells were incubated in amino acid-free medium with 10% dialyzed FBS for 1 or 24 h before the analyses. Unstarved and recovery conditions were run in XF DMEM (Agilent Technologies, 103575-100) with 25 mM glucose, 2 mM GlutaMAX (Gibco, 35050038), and 0.2% fatty acid-free BSA, while amino acid-starved conditions were run in amino acid-free medium with 0.2% fatty acid-free BSA, following incubation for 30 min. After measurement of basal respiration, compounds were sequentially injected as follows: 5 mM oligomycin, 1 mM isoproterenol, 7.5 mM FCCP, and 5 mM Ant A with 3 mM rotenone. Basal and maximal respiration are mean values of the 4 cycles before oligomycin injection and 3 cycles after FCCP injection, respectively. ATP-coupled respiration was calculated by subtraction of the value at the third measurement after oligomycin injection from the basal respiration. Proton-leak was calculated by subtraction of the mean of values after Ant A and rotenone from the value at the third measurement after oligomycin injection. Isoproterenol-response was calculated by subtraction of the value at the third measurement after oligomycin injection from the maximal value after isoproterenol injection.

Statistical analysis
GraphPad PRISM 6 and 8 were used for statistical analysis. Error bars, P values, sample size and statistical tests are detailed in the respective figure legends.
2.13. Data availability All data and materials are available upon request from the corresponding author.

RESULTS AND DISCUSSION
3.1. Brown adipose tissue is sensitive to energy deprivation by fasting To study the fasting response of metabolically important tissues, 8 weeks old chow diet (CD)-fed male wild-type C57BL/6 mice were fasted overnight. Overnight starvation significantly reduced blood glucose levels but did not significantly reduce body weight or weights of individual tissues in these lean CD-fed animals ( Figure 1A). Overnight fasting did not change the mRNA expression of the autophagy marker LC3b in BAT, or any other tissue investigated, despite a tendency for increased expression in subcutaneous and perigonadal white fat (SCF and PGF, respectively) ( Figure 1B). In contrast, expression of the brown/beige adipocyte specific genes uncoupling protein-1 (UCP-1) and the amino acid transporter PAT2 was significantly reduced ( Figure 1B). However, UCP-1 protein levels were not reduced following an overnight fast ( Figure 1C). PAT2 protein levels could not be determined due to the unavailability of specific antibodies for murine PAT2. Albeit we did not observe differences in the gene expression of LC3b upon fasting, a strong conversion from the cytosolic LC3 type I to the autophagosome incorporated LC3 type II was observed in skeletal muscle (tibialis anterior: TA) and BAT ( Figure 1C). Fasting upregulated LC3 levels in the liver, but did not result in increased conversion from LC type I to type II. These changes were not observed in SCF and PGF ( Figure 1C). The increase in LC3 type II in BAT but not white adipose tissues was also confirmed by immunofluorescence stainings of LC3 ( Figure 1D). Thus, BAT unlike WAT induces autophagy in response to an overnight fast similarly to skeletal muscle indicating a high sensitivity of BAT towards fluctuations in nutrient availabilities.

PAT2 localizes to the lysosome in brown preadipocytes
We previously identified PAT2 as highly expressed in brown and beige adipocytes [29] and the co-regulation with UCP-1 in response to an overnight fast prompted us to investigate its potential role in orchestrating the amino acid related fasting response. To study the function of PAT2 in brown preadipocytes and adipocytes, we established brown preadipocyte cell lines stably overexpressing HA-tagged PAT2 (PAT2-HA) or depleted of PAT2 (shPAT2) (Figure 2A and Fig. S1A). Using this overexpression system also allowed us visualizing the distribution of PAT2 due to the lack of a PAT2 antibody detecting the endogenous levels.
As previously reported [29], PAT2 mRNA expression is very low in preadipocytes and strongly induced upon brown adipocyte differentiation ( Figure 2A). Interestingly, protein levels of stably overexpressed PAT2 were also much lower in brown preadipocytes compared to fully differentiated mature brown adipocytes (Fig. S1A). PAT2-HA expression is under the control of the same promoter in both cell types. Thus, we suspected that brown adipocytes had a reduced degradation of PAT2 protein compared to preadipocytes. Lysosomal protein degradation is the default pathway for turnover of cell surface proteins and plays an important role in regulating mTORC1 activation and autophagy [36e39]. Indeed, overexpressed PAT2 predominantly localized to lysosomes in preadipocytes (Fig. S1B). Treatment of brown preadipocytes with the vATPase inhibitor bafilomycin A1 tended to increase PAT2-HA protein levels in preadipocytes (Fig. S1C) suggesting a continuous turnover via lysosomal degradation of PAT2 in preadipocytes.
3.3. PAT2 translocates from the plasma membrane to the lysosome upon amino acid depletion In contrast to preadipocytes, exogenously overexpressed PAT2 predominantly localized to the plasma membrane in mature brown adipocytes ( Figure 2B), consistent with our previous findings on the endogenous protein [29]. Knockdown or overexpression of PAT2 did not impair lipid accumulation (Figs. S2A and S2B) and expression of the key adipogenic transcription factor PPARg ( Figure 2C,D, Fig. S2C), Brief Communication despite increased mRNA levels of PPARg at day 8 in PAT2-HA brown adipocytes. In contrast, mRNA and protein levels of the brown adipocyte specific protein UCP-1 were elevated in both PAT2-HA and shPAT2 adipocytes compared to control cells ( Figure 2C,D, Fig. S2C). However, assessment of oxygen consumption rates in shPAT2 and PAT2-HA brown adipocytes did not reveal major differences between the cell lines. In contrast to our expectation we even observed a reduction in isoproterenol induced and FCCP induced maximal respiration of both shPAT2 and PAT2-HA brown adipocytes ( Figure 2E and S3A-E). These data could suggest a dissociation between UCP-1  Brief Communication 6 a translocation of PAT2 from the plasma membrane to the lysosome, which was rapidly reverted by replenishing amino acids ( Figure 2F). The selectivity of the observed translocation to amino acid depletion was confirmed by inducing translocation using amino acid withdrawal in the presence of 10% dialyzed FBS ( Figure 2G). Thus, PAT2 resides at the cell surface when ample extracellular amino acid levels are available but rapidly internalizes and translocates to the lysosome upon amino acid withdrawal. This raised the possibility that PAT2 contributes to the lysosomal amino acid turnover upon amino acid starvation.
3.4. PAT2 facilitates the recruitment of the V1 domain to assemble the vATPase upon amino acid starvation Assessment of intracellular pH, using a membrane permeable pH sensitive dye in brown adipocytes, transiently transfected with EGFP-LC3 to visualize autolysosomes, revealed decreased lysosomal pH in control cells following amino acid starvation, whereas this was strongly blunted in shPAT2 cells ( Figure 3A and Fig. S4A). PAT2-HA adipocytes showed decreased lysosomal pH already in regular culture conditions ( Figure 3A and Fig. S4A). To independently verify this observation we quantified proton dependent EGFP quenching in the autolysosome using a transiently transfected mCherry-EGFP-LC3 construct [41]. This showed reduced quenching of EGFP in shPAT2 and PAT2-HA adipocytes upon amino acid starvation for 1 h, when expressed as EGFP:mCherry ratio ( Figure 3B). Thus, loss of PAT2 increased lysosomal pH, whereas overexpression of PAT2-HA resulted in hyperacidification of the lysosome. Albeit, PAT2 in itself is able to transport protons, lysosomal acidification is thought to be predominantly driven by the vATPase [42]. Indeed, vATPase inhibition using bafilomycin A1 confirmed the dependency of starvation induced lysosomal acidification on vATPase activity in our cell lines (Fig. S4B). Hence, PAT2 regulates lysosomal pH via modulation of vATPase activity. Measurements of vATPase activity through pH dependent quenching of FITC-dextran [43] showed enhanced proton pumping in response to amino acid starvation in lysosomal enriched fractions of PAT2-HA cells. This was blunted in shPAT2 cells ( Figure 3C). To check the dependency on lysosomal PAT2 amino acid transport activity upon amino acid starvation, quenching experiments were performed with two PAT2 inhibitors, aMTP or 5HTP [44] in lysosomal fractions isolated from control brown adipocytes ( Figure 3D). Only higher concentration of aMTP suppressed quenching of FITC ( Figure 3D), but a similar inhibition was observed in PAT2 knockdown brown adipocytes, suggesting an off-target effect of the inhibitor at this concentration. However, both PAT2 inhibitors at 5 mM retained PAT2 at the cell surface upon amino acid starvation. Thus the amino acid transport activity is dispensable for the function of PAT2 in regulating vATPase activity but important for its cell surface localization (Fig. S4C). Regulation of vATPase assembly is the main mechanism regulating vATPase activity [45]. Western blots did not indicate differences in protein levels of V1B2 between shScr, shPAT2 and PAT2-HA adipocytes (Fig. S4D). Using blue native PAGEs we detected the fully assembled vATPase at >720 kDa, as determined by an overlapping signal of V1B2 and V0D1 ( Figure 3E). Both control and PAT2-HA cells increased the amount of fully assembled vATPase upon amino acid starvation, whereas shPAT2 cells had strongly decreased amounts of vATPase both at baseline and upon amino acid starvation. Thus, the observed increase in lysosomal pH in shPAT2 adipocytes appears as the consequence of impaired assembly of the full size vATPase, while the decreased pH upon overexpression of PAT2 could be attributed to a slightly increased vATPase assembly and proton pumping activity. Membrane and cytosolic fractionations independently confirmed reduced vATPase assembly upon amino acid depletion in shPAT2 adipocytes (Fig. S4E). Using co-immunoprecipitations we showed that PAT2-HA interacts with the V1B2 subunit of the vATPase V1 domain, but not V0D1, a V0 subunit, in amino acid starved adipocytes ( Figure 3F). However, unlike PAT1 [39], we were unable to identify an interaction of PAT2 with either mTOR or RagC ( Figure 3F). This suggests a mechanism whereby PAT2, upon translocation from the plasma membrane via the endosome to the lysosome, facilitates the assembly of the vATPase by recruiting the V1 domain to the lysosomal surface, where it interacts with the V0 subunit. Because of loss of PAT2, vATPase assembly is perturbed resulting in an overall decrease in lysosomal acidification. Conversely, overexpression of PAT2 increases vATPase assembly, activity and lysosomal acidification.
3.5. PAT2 regulates autophagosome size and mTORC1 activation upon starvation We then tested if the altered lysosomal acidification impairs autolysosome formation and function. Electron microscopy did not reveal differences in autophagosome numbers between the three cell lines ( Figure 3G,H). Thus, overexpression or depletion of PAT2 did not impair autophagosome formation per se. In line with this, we also did not observe differences in LC3 mRNA expression upon overexpression or depletion of PAT2 (Fig. S4F). However, differences in mean autophagosome size were observed upon starvation ( Figure 3I). Thus, we tested if PAT2 regulates autophagosome and lysosome fusion. Coimmunostainings of LAMP1 and LC3 revealed reduced colocalization of these lysosome and autophagosome markers, respectively, in both shPAT2 and PAT2-HA cells ( Figure 3J,K). Thus, both too high and too low lysosomal pH appear to inhibit autophagosome and lysosome fusion. To further investigate the impaired autolysosome function we studied mTORC1 re-activation upon prolonged amino acid depletion. Amino acid deprivation resulted in rapid dephosphorylation followed by rephosphorylation of the main mTORC1 effector protein S6K in control adipocytes ( Figure 3L and M and Fig. S4G). However, in line with impaired lysosomal pH and autolysosome formation, prolonged starvation resulted in impaired re-phosphorylation of S6K in both shPAT2 and PAT2-HA brown adipocytes ( Figure 3L,M). Phosphorylation of mTOR was not changed (Fig. S4G), suggesting that loss or gain of PAT2 alters autolysosomal amino acid release rather than growth factor signaling. Surprisingly, both knockdown and overexpression of PAT2, albeit showing opposing effects on vATPase assembly and lysosomal acidification, resulted in similar phenotypes with regards to autolysosome formation and mTORC1 re-activation. A similarly decreased S6K phosphorylation was also observed upon both knockdown [46] and overexpression [39] of PAT1, possibly due to impairments of amino-acid dependent re-location of mTOR to the lysosome [47] and prevention of amino acid accumulation in the lysosomal lumen activating mTORC1 [39], respectively. In contrast to PAT2, PAT1 and SNAT family members constitutively localize to the lysosomal membrane where they interact with the mTORC1 supercomplex requiring vATPase [39]. However, the role of PAT2 appears more indirect as PAT2-HA could not be co-precipitated with either mTOR or RagC in control or amino acid starved conditions in brown adipocytes ( Figure 3F). This suggests that PAT2 regulates mTORC1 activity via its function on vATPase assembly, lysosomal acidification and thereby lysosomal amino acid release.
To understand the bioenergetic consequences of loss and gain of PAT2 in brown adipocytes we assessed oxygen consumption and uncoupled respiration in shScr, shPAT2 and PAT2-HA brown adipocytes ( Figure 3N and S3A-E). One-hour amino acid deprivation did not induce significant changes between the cell lines. Twenty-four-hour amino acid starvation, which is associated with impaired mTORC1 activity in shPAT2 and PAT2-HA adipocytes, led to a defective respiration in shPAT2 adipocytes, with a similar trend in PAT-HA adipocytes ( Figure 3N). Starvation did not alter expression of either PAT2 or PAT2 in either cell line compared to the unstarved condition. Expression of PAT1 was increased in shPAT2 cells in the unstarved condition compared to control and PAT2-HA cells, but its expression was not different among the three cell lines upon 24 h amino acid starvation (Fig. S4H). When adipocytes were measured in amino acid-containing medium following a 24 h-amino acid deprivation, both shPAT2 and PAT2-HA cells showed reduced oxygen consumption compared to control cells. The effect of either knockdown or overexpression of PAT2 on lysosomal acidification and vATPase assembly were opposed, whereas the impact on autophagolysosome formation and mTORC1 activation was similar. Thus it is very likely that the observed impact on mitochondrial activity is a consequence of either impaired starvation induced mTORC1 activity or autophagy. The latter could suggest a role of PAT2 in regulating mitochondrial turnover via regulating mitophagy.

CONCLUSIONS AND LIMITATIONS
In summary, we identified the amino acid transporter PAT2 to promote lysosomal acidification upon reductions in extracellular amino acid availability in brown adipocytes. In response to amino acid depletion, PAT2 translocates from the plasma membrane to the lysosome, where it facilitates the assembly of the vATPase promoting lysosomal acidification, which is essential for the induction of autophagy. However, our study also revealed that PAT2 protein levels need to be tightly regulated as both too little (as seen in the knockdown) or too much (as seen in the overexpression), impair autophagosome and lysosome fusion. Thus, the expression of PAT2 in brown adipose tissue and the here described functions suggest that BAT has a very high sensitivity towards changes in extracellular amino acid levels to induce autophagy. It will be very important to investigate the consequences of loss or gain of function of PAT2 on mitophagy, mitochondrial turnover and the overall thermogenic capacity of brown adipocytes in the future. However, the current study also has technical limitations that need to be considered when assessing the role of PAT2 in vivo, especially in humans. We were unable to study the endogenous protein levels and dynamics due to a lack of a mouse specific PAT2 antibody. Thus, we will continue our efforts to create a murine PAT2 specific antibody to confirm the endogenous protein translocation. Moreover, we performed all experiments in an immortalized brown adipocyte cell line and stable knockdown or overexpression of PAT2. Thus, future studies using PAT2 knockout or PAT2 overexpressing mice will be important to also assess the physiological role of PAT2 in BAT in vivo.

AUTHOR CONTRIBUTIONS
JW, YO and SU designed and conducted experiments and wrote the manuscript. RK and IS conducted experiments. MK conducted the electron microcopy experiments. YL and JO performed cellular respirometry. RF and SMH contributed to study design and data analysis.