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Tissue-Imaging of Glutathione-Specifical Naphthalimide-Cyanine Dye with Two-Photon and Near-Infrared Manners.

Molecular probes suitable for different fluorescence imaging technologies can meet the requirements of different scientific researches in biological applications. In this work, a naphthalimide-cyanine based sulfonamide was used to specifically visualize the glutathione of mouse tissues with a two-photon manner for the naphthalimide moiety and a near-infrared manner for the cyanine moiety, respectively. The results showed that this probe served as a dual-model tissue-imaging agent for the visualization of glutathione with around 200 μm imaging depth in two-photon manner and 120 μm imaging depth in near-infrared manner, respectively, which provided a model for the tissue-imaging in visible and near-infrared channels.