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Batch CGTase Production with Free and Immobilized Bacillus firmus Strain 37 in Bovine Bone Charcoal

The present study aimed to study the batch production of CGTase 
(cyclomaltodextrin-glucanotransferase) 
with Bacillus firmus strain 37 free and immobilized in bovine bone charcoal 
in batch mode and in a fluidized bed 
batch reactor, respectively. The bovine bone charcoal is an innovative support 
material for the immobilization of microorganisms’ producers of enzymes and the 
use of this microbial support allows its reuse to a significant cost reduction 
of the process. The batch fermentation with free cells was investigated for 96 
h and reached a CGTase activity equal to 0.77 U/mL. When the microorganism was 
immobilized on bovine bone charcoal (7 g) and cultivated in fluidized bed batch reactor with air 
supplementation (1 volume of air/volume of medium * minute), the same activity 
could be achieved in 24 h. The results of enzymatic activity achieved show the potential of CGTase production in a 
short time with Bacillus firmus strain 37 immobilized in bovine bone charcoal matrix and using air 
supplementation in the production medium.