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Improvement in Abrasive Biopsy Sampling of Tooth Enamel

The abrasive biopsy technique for removal of enamel in vivo was modified by controlling the biopsy area, polishing speed, and pressure. These modifications resulted in greater uniformity of samples obtained by a single operator, as well as between different operators. A mean depth of 2.5 μm was obtained within the standardized area of the controlled biopsies.