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Surgical model for functional intravital microscopy of the lung using an acute window

Numerous pathologies of the lung could benefit from a model of functional microscopic imaging of the lung in laboratory animals. Recent developments in window chamber microscopy enable us to measure key parameters of the lung including capillary blood flow and hemoglobin saturation. Functional intravital microscopy at high magnification, however, requires a still image for extended exposure times which is very difficult to obtain in the lung due to respiratory and cardiac movement.