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Phosphorylation, Oligomerization and Self-assembly in Water Under Potential Prebiotic Conditions

Prebiotic phosphorylation of (pre)biological substrates under aqueous conditions is a critical step in the origins of life. Previous investigations have had limited success and/or require unique environments which are incompatible with the subsequent generation of the corresponding oligomers or higher order structures. Here we demonstrate that diamidophosphate (DAP), a plausible prebiotic agent produced from trimetaphosphate, efficiently (amido)phosphorylates a wide variety of (pre)biological building blocks (nucleosides/tides, amino acids, and lipid precursors) under aqueous (solution/paste) conditions, without the need of a condensing agent. Significantly, higher order structures (oligonucleotides, peptides and liposomes) are formed under the same phosphorylation reaction conditions. This plausible prebiotic phosphorylation process under similar reaction conditions could enable the systems chemistry of the three classes of (pre)biologically relevant molecules, and their oligomers, in a single-pot aqueous environment.

Phosphorylation of (pre)biological molecules in water has been a long-sought goal in prebiotic chemistry. Here, it is demonstrated that diamidophosphate phosphorylates nucleosides, amino acids and glycerol/fatty acids in aqueous medium, while simultaneously leading to higher-order structures (oligonucleotides, peptides and liposomes) in the same pot.
Phosphorylation of biologically relevant molecules under potential prebiotic conditions is an important step in the origins of life and has been investigated (especially for nucleosides) over a wide variety of conditions 1-4 with more recent variations 5,6 . All of these approaches use differing phosphorylation sources and are limited in substrate scope due to their reaction conditions, or require unique non-aqueous environments to enable the phosphorylation process 7,8 . Many of these approaches are, therefore, incompatible with next step of generating the corresponding oligomers and higher order structures (towards RNA or pre-RNA worlds) from these phosphorylated substrates, necessitating spatially separated processes and other discrete mechanisms and chemistries. A universal and efficient phosphorylating agent which would phosphorylate a wide class of (pre)biological molecules in water under similar reaction conditions, and enable formation of higher order structures, would be of significance in the context of prebiotic systems chemistry 9 .
We have previously shown that diamidophosphate (DAP, Fig. 1) efficiently phosphorylates a wide variety of prebiotically relevant sugar molecules and their building blocks 10 . A prebiotic synthesis of phosphoenol pyruvate has been recently demonstrated using DAP as the prebiotic phosphorylating reagent 11 . In all cases, the mechanism involves a nucleophilic attack of NH 2 -group of DAP on the free aldehyde moiety to facilitate an intramolecular phosphorylation of the α-hydroxy-aldehydes ( Supplementary Fig. 1). Such a mechanism precludes the phosphorylation of hydroxyl groups which are not next to a carbonyl moiety (e.g. nucleosides and glycerol). However, when we observed that DAP alone in water at pH 6, or in the presence of ortho-or pyro-phosphates, produced trimetaphosphate (Supplementary Fig. 2-5), it suggested that direct phosphorylation of other nucleophilic groups in aqueous medium should be possible by a direct attack on the phosphorous center with the protonated NH 2 -group of the DAP acting as the leaving group 12 .
The phosphorylation proceeds through nucleophilic attack of the 2',3'-cis-hydroxyl groups on the protonated DAP leading to a putative monophosphoramidate (2'-NMP NH2 or 3'-NMP NH2 ) intermediate, which then cyclizes to give the 2',3'-cyclophosphate derivatives 5-8. When imidazole is present, the protonated DAP is converted to the amidophosphoimidazolide ( Supplementary Fig. 7), which reacts further. Support for these intermediates was obtained from 31 P-NMR using 15 N-labeled reactants . This phosphorylation chemistry, in principle, can be extended to other alternative (prebiotically plausible) nucleosides that have the adjacent cis-hydroxyl configuration, 17 a work that is ongoing.
The phosphorylation-followed-by-hydrolysis raises the possibility of using DAP as a (re)activating agent for 5'-, 2'-, and 3'-nucleoside phosphates in water for ligation/ oligomerization reactions, providing an alternative to the current methods of activation in prebiotic oligomerization and replication studies 19 . Phosphorylation of selected oligonucleotides with 5'-phosphate and 3'-phosphate terminal ends were briefly investigated, and found to produce the respective oligo-5'-DP NH2 and the corresponding oligo-2',3'-cyclophosphate (via the oligo-3'-DP NH2 ), paralleling the observations made in the mononucleotides (Fig. 1d, Supplementary Tables 10-11). Investigations probing the scope of DAP for template-mediated ligation/oligomerization are ongoing.

Phosphorylation of glycerol and fatty acids by DAP leading to (self-assembly) vesicles.
Since cis-disposed 2',3'-hydroxyl groups of nucleosides are converted to cyclophosphates, glycerol 23 was reacted with DAP with the purpose of accessing the building blocks of phospholipids (Fig. 2a). Mixing 23 with DAP and imidazole in water at r.t. led to the formation of glycerol-1,2-cyclophosphate 24 (≈ 12%); with the paste-reaction demonstrating efficient conversion (≈ 60%) via the amidophosphorylated intermediate (24i , Supplementary  Table 12). Heating the paste reaction (50 °C) accelerated the reaction, but also led to the formation of the diglycerol-phosphodiester, indicating that the cyclophosphate is attacked by another molecule of glycerol. Based on the studies of Deamer 20 , we probed the ability for DAP to mediate ester bond formation by reacting nonanol with ammonium acetate under paste-reaction conditions, and found that DAP increased the efficiency of ester bond formation when compared to the control ( Supplementary Fig. 117, Table 13). Encouraged by these observations, we performed a one-pot paste-reaction with nonanoic acid 25 20 and glycerol in the presence of DAP and imidazole (Fig. 2b). Analysis of the crude reaction mixture suggested the formation of a cyclophospholipid 26, the structure of which was confirmed by comparison with synthesized authentic 26, (Fig. 2c Fig. 125). In addition, we were pleasantly surprised to find also TEM images implying the formation of giant bilayer vesicle-like structures with diameters on the average of 9.2 µm (Fig. 2d) with some structures nearing 20 µm (Supplementary Fig. 126). The control reaction lacking DAP, but containing only nonanoic acid and glycerol (Supplementary Figure 133), or each of the components alone (for example, nonanoic acid alone), did not show the formation of these "vesicle-like" structures ( Supplementary Figs. 132-135). The opaque solution generated from the synthetic cyclophospholipid 26 in water was analyzed by TEM which showed "vesicle-like" structures ( Fig. 2e) comparable to those obtained from the crude paste-reaction mixture. This demonstrates that cyclophospholipid 26, generated in the crude paste-reaction mixture, is indeed capable of forming these vesicle-like structures -perhaps even better in heterogeneous mixtures with glycerol 20 . To prove that the structures are indeed liposomes, we performed the dye-inclusion experiments 20 , wherein the confocal microscopy images showed that the red-rhodamine dye was retained within the bilayer, while the green-pyranine dye was encapsulated within the cavity of the liposome (Fig. 2g and 2f). Preparing the liposomes with both dyes afforded a merged-image suggesting an "uneven-enclosed" structure ( Fig. 2h), probably from the changes in internal osmotic pressure 22 and interlayerdistances 23 when both dyes are present. Preliminary reactions of octanoic acid with DAP under the paste-reaction conditions gave rise to smaller vesicle-like structures ( Supplementary Fig. 131). That a library of prebiotically plausible fatty acids, available through meteoritic delivery 24 or other prebiotic means 20 could form stable phospholipid liposomes such as giant-vesicles by phosphorylation in the presence of glycerol, while unprecedented, does align with the advantage provided by the physical and chemical heterogeneity in vesicles in the context of protocells 25 .

Phosphorylation of amino acids by DAP leading to oligopeptides.
Motivated by these observations we briefly explored -as a proof of principle -the phosphorylation of carboxylic acids of three representative prebiotic amino acids 27-29 (Fig.  3a) under aqueous conditions 3 . When glycine 27 was reacted with DAP in water, quantitative phosphorylation of the α-amino and the α-carboxyl groups was observed (Figs. (DKP) was also detected. With Gly-Gly 30, the formation of oligomers up to an octamer was observed by mass spectrometry (Supplementary Fig. 144). The reaction of DAP with aspartic acid 28 and glutamic acid 29 (Fig. 3a) also showed the formation of oligopeptides up to tetramers ( Supplementary Figs. 145 & 147). In the case of 28, 1 H-NMR shows ca. 23% conversion to higher order products with minimal DKP (Supplementary Fig. 146), while for 29, it was ca. 15% conversion to higher order products (DKP + peptides, Supplementary Fig. 148). Accurate determination of oligopeptide yields (currently complicated by the interference from the various phosphorylated species), optimization of reaction conditions for increasing oligopeptides yields, the nature of mechanistic pathways, and elucidation of the nature of connectivity (α-versus β-) are some of the issues that need to be addressed, and are underway. The promising preliminary results of oligopeptide formation in water warrant in-depth and systematic investigations to determine the scope and selectivity of the oligopeptide forming process, and to address the issues associated with random peptide synthesis such as the rapid increase in diversity of even short peptide sequences 29 .

Discussion
The similar phosphorylation-conditions for all three classes of prebiological molecules (nucleosides, fatty acids and glycerol, and amino acids) suggest that they could be combined and conducted in a single-pot. Moreover, the commonality of conditions for the oligomerization of different building blocks suggests that productive and mixed chemistries might be possible, [30][31] such as cross-catalyzing the oligomerization and self-assembly process, and the resulting higher order structures could, in turn, increase the efficiency of phosphorylation. This approach could create opportunities which go beyond the generation of phosphorylated building blocks, leading to the co-formation and co-existence of building blocks and their oligomers in the same locality, conducive for the emergence of primordial synergistic systems within confined (aqueous) environments-towards an RNA or pre-RNA world(s). Though there are similarities with extant biochemical pathways using such P-N activation chemistries 32 (such as N-phosphoryl transfers [27][28], any comparison must be viewed with caution given the pitfalls of extrapolating extant biochemical pathways backwards all the way to prebiotic chemistry and vice-versa. 33 It has been conjectured that combining the nitrogenous and oxygenous versions of prebiotic molecules (as in TNA and its nitrogenous versions 34 ), could provide a library of alternative opportunities 32, . The results from this work and previous DAP-phosphorylation investigations 10,11,35 , demonstrate that such an expansion of the phosphorylation scenario to include the prebiotically plausible nitrogenous version of phosphate (such as DAP) provides an efficient and alternative solution to the prebiotic-phosphorylation-problem. While DAP has been used as a prebiotically plausible reagent 11,35 , it has been produced from the prebiotically available trimetaphosphate 36 by reaction with ammonia, 37-38 at relatively high pH. Since the results demonstrated in this work proceed at lower pH, investigation of additional, prebiotically plausible and alternative pathways at milder pH, such as corrosion of phosphide minerals (schreibersite) [39][40] , towards the formation of DAP would be desirable. In that context, the detection of PN containing compounds in interstellar medium [41][42] and star forming regions 43 suggests that nitrogenous versions (or forerunners) of phosphate may not be unreasonable to contemplate 32,44 . The phosphorylation of small molecules by DAP and its analogs, may also prove to be useful in synthetic chemistry. 32

Methods Summary (full details are given in the Supplementary Information)
General phosphorylation protocols: DAP in water: To the substrates (nucleosides, nucleotides, oligonucleotides, glycerol, nonanoic acid or amino acids) in water, DAP, with or without imidazole (and/or zinc chloride and/or magnesium chloride), was added. The pH was adjusted and maintained between 5.5-8 by the addition of aq. 4M HCl and was agitated at room temperature. Additional DAP was added based on the progress of the reaction and its consumption as monitored by NMR analyses. DAP in "paste-reaction": The substrates (nucleosides, nucleotides, oligonucleotides and glycerol) and DAP, with or without imidazole, were ground together with few drops of water. Additional DAP was added based on the progress of the reaction and its consumption as monitored by NMR analyses.

Phosphorylation reaction analyses
The progress of the reactions was monitored by the following techniques: 1 H, 13 C and 31 P NMR spectroscopy (nucleosides, nucleotides, glycerol, fatty acids and amino acids); FPLC (oligonucleotides); LC-MS (glycerol and amino acids); MALDI-Tof (oligouridylate, oligonucleotides and amino acids)

Vesicle preparation
Fatty acid (1 equiv.), glycerol (1 equiv.), DAP (5 equiv.), imidazole (5 equiv.) were mixed together with few drops of water and left at room temperature. An aliquot of this crude reaction mixture was mixed with water, vortexed (with and without sonication and filtration) to allow for the formation of micelles/vesicles.

Vesicle characterization
The structures obtained from the vesicle preparation described above (and also from synthetic phospholipid 26) were characterized by dynamic light scattering and visualized by transmission electron microscopy with negative staining, and confocal laser scanning microscopy with dye incorporation.
The data that support the findings if this study are available from the corresponding author upon reasonable request.