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Haematoxylon brasiletto Extracts Inhibit Growth, Enterotoxin Production, and Adhesion of Vibrio cholerae

The effects of Haematoxylon brasiletto extracts on growth, enterotoxin production, and adhesion of V. cholerae O1 and O139 to Chinese Hamster Ovary (CHO) cells were determined. The minimal bactericidal concentration (MBC) for growth was 0.3–0.4 mg mL–1. No enterotoxin formation was detected when lower concentrations (50% or 75% of the MBC) of H. brasiletto extracts were added to the media. Pre-exposing bacteria and CHO cells to various concentrations of extracts affected the adhesion between bacteria and CHO cells. Partial purification of the active fraction suggested that polyphenols may play a role in the antimicrobial activity exhibited by H. brasiletto extracts.