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The ESCRT-II proteins are involved in shaping the sarcoplasmic reticulum in C. elegans

ABSTRACT The sarcoplasmic reticulum is a network of tubules and cisternae localized in close association with the contractile apparatus, and regulates Ca2+ dynamics within striated muscle cell. The sarcoplasmic reticulum maintains its shape and organization despite repeated muscle cell contractions, through mechanisms which are still under investigation. The ESCRT complexes are essential to organize membrane subdomains and modify membrane topology in multiple cellular processes. Here, we report for the first time that ESCRT-II proteins play a role in the maintenance of sarcoplasmic reticulum integrity in C. elegans. ESCRT-II proteins colocalize with the sarcoplasmic reticulum marker ryanodine receptor UNC-68. The localization at the sarcoplasmic reticulum of ESCRT-II and UNC-68 are mutually dependent. Furthermore, the characterization of ESCRT-II mutants revealed a fragmentation of the sarcoplasmic reticulum network, associated with an alteration of Ca2+ dynamics. Our data provide evidence that ESCRT-II proteins are involved in sarcoplasmic reticulum shaping. Summary: We report that endosomal sorting complex required for transport ESCRT-II proteins are required for the maintenance of the sarcoplasmic reticulum integrity in C. elegans body wall muscle cells.