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Biotechnological conversion of spent coffee grounds into lactic acid

This work investigates the potential bioconversion of spent coffee grounds (SCG) into lactic acid (LA). SCG were hydrolysed by a combination of dilute acid treatment and subsequent application of cellulase. The SCG hydrolysate contained a considerable amount of reducing sugars (9·02 ± 0·03 g l−1, glucose; 26·49 ± 0·10 g l−1 galactose and 2·81 ± 0·07 g l−1 arabinose) and it was used as a substrate for culturing several lactic acid bacteria (LAB) and LA‐producing Bacillus coagulans. Among the screened micro‐organisms, Lactobacillus rhamnosus CCM 1825 was identified as the most promising producer of LA on a SCG hydrolysate. Despite the inhibitory effect exerted by furfural and phenolic compounds in the medium, reasonably high LA concentrations (25·69 ± 1·45 g l−1) and yields (98%) were gained. Therefore, it could be demonstrated that SCG is a promising raw material for the production of LA and could serve as a feedstock for the sustainable large‐scale production of LA.