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A comparative study on citric acid production kinetics of two Yarrowia lipolytica strains in two different media

Microbial production of citric acid was performed by Yarrowia lipolytica NBRC 1658 and a domestic strain in the media containing glucose or fructose as substrates. The study was carried out in a batch system. The non-competitive substrate inhibition model was proposed for growth of the yeasts in both substrate media. Higher dry mass and lower K s values were obtained in glucose media when compared to fructose media. The highest citric acid concentration (65.1 g/L) was obtained with the domestic strain in the medium containing 200 g/L fructose. In the media containing fructose, maximum citric acid concentration and productivity values determined with the domestic strain were aproximately two-fold greater than those obtained with NBRC 1658 strain. The required initial concentration of glucose or fructose at which best citric acid production properties were observed changed between 100-200 g/L for both of the strains. The ratio of citric acid to isocitric acid was found to change between 11.70-16.62.