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Induction of Anticarcinogenic Marker NQO1 Enzyme by Soybean‐derived Glyceollins in Colorectal Cancer Cells

Glyceollins, a family of phytoalexins, are de novo synthesized from daidzein in the soybean upon exposure to some types of fungus. Glyceollins have been reported to induce antioxidant and phase II detoxifying enzymes and thereby prevent some types of tumors induced by chemical carcinogens. However, it is not well established yet whether glyceollins could induce phase 2 detoxifying enzymes and prevent colon tumorigenesis. In this study we investigated inhibitory effects of glyceollins on the proliferation of colorectal cancer cell lines such as HCT116 (p53+/+), HT29 (p53‐/‐), and Caco‐2 by MTT assay and DAPI staining. In addition, we investigated whether glyceollins induce phase 2 detoxifying enzyme through Nrf2 signaling pathway. While glyceollins had marginal effect on the proliferation of colon cancer cells, they were found to induce phase 2 detoxifying enzymes in dose‐dependent and cell type‐specific manners, suggesting their potential preventive activity against colon tumorigenesis (supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (Project No. 2014R1A2A2A01005773).