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Angiotensin II modulates vascular smooth muscle cells alpha‐1D‐adrenoceptors

Angiotensin II plays an important role in regulating systemic arterial pressure, provokes cardiovascular remodelling and hypertension, as well as an increase in alpha1‐adrenoceptors mRNA and protein. The aim of this work was evaluate the influence of angiotensin II on alpha 1D‐adrenoceptors in isolated smooth muscle cells of Wistar rat aorta. Smooth muscle cells were incubated with angiotensin II (100 nM) for different periods of time (30min–24hrs), and then protein by Westernblot was determined for the alpha 1‐adrenoceptor subtype.