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Automated system for analytical microbiology. 3. Computer interpolation of potency.

Highly accurate photometric assays for antibiotic substances, such as those obtainable with a system composed of automated components, require an accurate method of obtaining potencies of samples. The usual manual interpolation of sample potencies from a standard dose-response line may introduce an error of several percent. Errors can come from the use of an inappropriate dose-response line and from the inability of the person interpolating to differentiate potencies with a resolution of less than 1%. A new dose-response line for manual interpolation of antibiotic potencies is described. Interpolations by means of a digital computer from that equation and from the relation log A versus C were both shown to reduce errors considerably. In addition, the computer may be put on-line and provide a typed report as soon as the last tube in an assay is processed.