{'fraction_non_alphanumeric': 0.027056277056277056, 'fraction_numerical': 0.0010822510822510823, 'mean_word_length': 4.967741935483871, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
RT-PCR: 'background priming' during reverse transcription.

The reverse transcriptase-polymerase chain reaction (RT-PCR) technique is a highly sensitive method for detecting and characterizing RNA molecules in small samples of cells or body fluids. It employs reverse transcription of the target RNA followed by PCR amplification of its cDNA. Widely used primers for cDNA synthesis are oligo (dT), random hexanucleotides or a sequence-specific antisense oligonucleotide primer. In addition it has been shown that low molecular weight cellular RNAs can serve as primers for cDNA synthesis (1). Here we report that random priming by cellular RNAs also plays an important role in amplifying RNA molecules during RT-PCR. Due to random priming at the level of reverse transcription (RT) a pool of cDNAs of different sequence origin is synthesized, and it has to be considered that it is this pool of cDNAs PCR is accomplished on.