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Applications of dynamic light scattering, fluorescence microscopy and fluorescence spectroscopy in DB-67 liposomal formulation studies

Campthothecin (CPT) and its analogues as prominent anticancer agents are currently the subject of the intensive studies. One of the most promising camptothecin analogues is 7-tert-butyldimethylsil- 1 0-hydroxycampthothecin called DB-67. It is characterized by high affinity to SUV (small unilamellar lipids vesicles) and relatively high stability in human blood. The studies of liposomal formulation as a delivery systems for DB-67 are the subject of this paper. The methods of dynamic light scattering (DLS), fluorescence microscopy (FM) and fluorescence spectroscopy (FS) are used to determine the physical properties of DB-67 liposomal formulation.