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An anatomical screening of white jute accessions for fibre content

The present study was conducted to evaluate the performance of 24 advanced lines of white jute and four check varieties ( CVL-1, CC-45, BJRI Deshi Pat-7 & BJRI Deshi Pat-8) for screening out high yielding and better quality fibre through an anatomical approach at JAES, Manikganj. The experiment was conducted in randomly complete block design (RCBD) with three replications. The cultivars revealed significant differences among them for all the characters with range of variability. The highest bark thickness (2.32 mm) was noticed by the accession C-5036 which performed significantly better than the two checks and lowest was recorded by C-5126 (1.07 mm). The variety BJRI Deshi Pat-8 observed significantly the highest number of pyramid (54.13) whereas the lowest was recorded by the varieties BJRI Deshi Pat-7 (28.23). The accession C-5117 showed significantly the highest number of layer (10.29) whereas the variety, BJRI Deshi Pat-7 showed significantly lowest number of layer (6.32). The accession C-2305(92.37) and C-12221 (91.19) significantly showed top two number of fibre bundle, however, the lowest number of fibre bundle was recorded by BJC-5105(42.79). The largest area of pyramid was observed by the accession C-5036 (46.85 sq. mm) whereas the lowest was showed by BJC-5105 (14.50 sq. mm). The differences between Phenotypic Coefficient of Variation (PCV) and Genotypic Coefficient of Variation (GCV) were little for all the characters indicating these characters were less influenced by the environment. Heritability and genetic advance (GA) were high for number of pyramid, number of fibre, bundle and Area of pyramid (sq.mm). Three accessions(C-12221, C-2305, C-5036) are showed the best performance than other accession on our screening program. These accessions are included in cluster V among the five clusters which exhibit the highest performance in all parameter except number of pyramid and may be used in further hybridization of jute.


INTRODUCTION
Jute, the bast fibre, is obtaining from the bark of two cultivated species of the genus namely Corchorus capsularis L. and Corchorus olitorius L. of the family Tiliaceae. Jute, the 'Golden Fiber' of Bangladesh, is contributing about 4% GDP to the national economy and earns about 5% of the foreign exchange as well [1]. Bangladesh ranks the second position after India in area coverage and production of jute [2]. According to the statistical reports of [3], The area of Jute cultivation in Bangladesh is 17, 51,325 acres (7, 08,723 hactares) of land where the total production is 83, 95,840 bales per annum with an average yield of 4.79 bales per acre (11.846 bales per hectare) White jute (Corchorus capsularis L.) can grow both in low and high land and has better adaptability than the other cultivated species. Jute fibre is obtained from the bast or phloem layer (sclerenchymatous cells) of the stem of two species of the genus Corchorus namely Corchorus capsularis L. and Corchorus olitorius L. The fibres are grouped into bundles and are arranged in trapezoid wedges (pyramid) and alternate with medullary rays of soft tissue. The fibre bearing potentiality of jute plants largely depends on the number of fibre trapezoids, size of fibre bundles and their compactness [4]. Several workers have studied the compactness of fibre bundles of different species of Corchorus [5,6,7] and some of them reported that the area of cell/section has the highest significant correlation with fibre yield/plant [8].
Bast fibres are part of vegetative biomass as they are formed in the secondary phloem region in jute stem. Yield of bast fibres which are produced within the bark of the stem of bast fibre crop can't be assessed unless the plants are retted and fibres extracted. This procedure of yield assessment is long waiting and costly. So, we can easily isolate higher fibre bearing

ABSTRACT
The present study was conducted to evaluate the performance of 24 advanced lines of white jute and four check varieties ( CVL-1, CC-45, BJRI Deshi Pat-7 & BJRI Deshi Pat-8) for screening out high yielding and better quality fibre through an anatomical approach at JAES, Manikganj. The experiment was conducted in randomly complete block design (RCBD) with three replications. The cultivars revealed significant differences among them for all the characters with range of variability. The highest bark thickness (2.32 mm) was noticed by the accession C-5036 which performed significantly better than the two checks and lowest was recorded by C-5126 (1.07 mm). The variety BJRI Deshi Pat-8 observed significantly the highest number of pyramid (54.13) whereas the lowest was recorded by the varieties BJRI Deshi Pat-7 (28.23). The accession C-5117 showed significantly the highest number of layer (10.29) whereas the variety, BJRI Deshi Pat-7 showed significantly lowest number of layer (6.32). The accession C-2305(92.37) and C-12221 (91. 19) significantly showed top two number of fibre bundle, however, the lowest number of fibre bundle was recorded by BJC-5105(42.79). The largest area of pyramid was observed by the accession C-5036 (46.85 sq. mm) whereas the lowest was showed by BJC-5105 (14.50 sq. mm). The differences between Phenotypic Coefficient of Variation (PCV) and Genotypic Coefficient of Variation (GCV) were little for all the characters indicating these characters were less influenced by the environment. Heritability and genetic advance (GA) were high for number of pyramid, number of fibre, bundle and Area of pyramid (sq.mm). Three accessions(C-12221, C-2305, C-5036) are showed the best performance than other accession on our screening program. These accessions are included in cluster V among the five clusters which exhibit the highest performance in all parameter except number of pyramid and may be used in further hybridization of jute. potentiality genotypes through anatomical studies i.e. fibre compactness per unit area of plant bast. The quality of jute fibre depends mainly on the length and length-breadth ratio of ultimate fibres. The ultimate fibre of jute varies from 500 to 6500 μm in length and 10 to 30μm in diameter [9]. The length-breadth ratio of ultimate fibres for fine yarn production should be about 1000-2000 [10] but in jute this ratio is an average only about 120 [9]. Many workers studied the ultimate fibre cells of different varieties of Corchorus olitorius L [6,11]. The present study was undertaken for selection of cultivars of white jute having high fibre bearing potentiality based on anatomical screening approach.

METHODS AND MATERIALS
23 advanced lines of white jute and four released varieties namely, CVL-1, CC-45, BJRI Deshi Pat-7 & BJRI Deshi Pat-8 were used as experimental materials. The cultivars were grown at the JAES, Manikganj in a randomized complete block design with three replications. A plot for each genotype was represented by 3 rows of 3.6 m length with a spacing of 30 cm x 5 cm. Standard agronomic practices were followed. At the age of 110 days of maturity, five plants were selected randomly from each replication for harvest. The middle portion of stem (5cm stem piece) were collected and preserved in Formalin acetic acid alcohol solution (10 ml of formalin, 5 ml 85% glacial acetic acid and 85 ml of 70% alcohol) for anatomical study. The preserved sample were taken out and washed. About 20-25μm thick transverse sections (TS) was done with the help of a microtome machine for preparing slide. The slides were studied under compound microscope (4X10 magnifications) and data were recorded for bark thickness, number and area of phloem wedges, number of fibre bundle layers and number of fibre bundles per section. Plant weight, base diameter and dry fibre weight of the materials were also recorded.
Meceration of fibre cell: The fibre was extracted following the method described by Aziz et al. 1964 with little modification. The anatomical data were recorded from 5-10 randomly selected plants of each genotype from each replication at the time of harvest (110 days of crop age). Data were recorded on date of sowing, germination, 1 st and average flowering. Bark diameter, bark thickness, number of trapezoids/section were calculated for three plants of each accession under microscope. Area of trapezoid/section (μm): Area of trapezoid was measured by the following formula: (Area of trapezoid = ½ (Upper length + Lower length) X perpendicular length of trapezoid X conversion factor [Conversion factor = 39.4 in 4 X 10 magnification], number of layers/ trapezoid, number of bundles/trapezoid were calculated under microscope from randomly selected trapezoid. Length, breadth and length: breadth ratio to be measured for 10 randomly selected the fibre cells by using oculometer under the microscope Analysis of variance and covariance were done according to [12]. Coefficient of variation (PCV and GCV) and heritability (H2b) were calculated by the formulae suggested by [13 and 14], respectively. Genetic advance (GA) was derived by the formula given by [15] A cluster diagram was drawn according to [16] that gave a brief idea of the pattern of diversity among the genotypes included in a cluster.

RESULT AND DISCUSSION
The analysis of variance of different advanced lines of white jute for anatomical characteristics is shown in Table 1. Analysis of variance shows that the difference among genotypes for all the traits under study viz., Bark thickness (mm), Number of pyramid, Number of layer, Number of fibre Bundle and Area of pyramid (sq.mm) were highly significant.
The mean performance of 24 advanced line and four check varieties for five anatomical characteristics are shown in Table 2.
Among the accession and varieties, significantly the highest bark thickness (2.32 mm) was found from the accession C-5036 which was followed by accession C-12221 (2.23 mm) and lowest was recorded by C-5126 (1.07 mm). Bark thickness is an important character for fibre yield of Jute accession or varieties in case of the higher bark thickness produce the better thickness of fibre which will ensure the higher production of fibre. In our findings, the great variation was observed by both the accession and varieties where the accession showed better performance than the check varieties. These results are supported by the findings of Pervin and Haque (2012), Haque et al. (1977).The varieties BJRI Deshi Pat-8 showed significantly the maximum number of the pyramid (54.13) followed by C-1831x A-38 (42.78). On the contrary, significantly the lowest number of the pyramid was noticed by the varieties BJRI Deshi Pat-7 (28.23) which was closely followed by C-2236 (29.70). It was found that the accession C-5117observed significantly the maximum number of layer (10.2) which was followed by accession C-12221(9.76). On the other hand, the variety, BJRI Deshi Pat-7 showed significantly the lowest number of layer (6.32) followed by the variety CC-45 (6.96).The two anatomical characters including the number of the pyramid and the number of layers was significant with the mean performance of fibre yield. Though one of the varieties recorded significantly the highest number of the pyramid, the highest number of the layer was recorded by an accession significantly. So, both the number of pyramid and number of the layer can be used as compatible criteria for screening of jute through the anatomical approach. The Results of [17,18,19] were also in agreement with our findings. The highest number of the fibre bundle (92.37) was observed by the accession C-2305 followed by accession C-12221 (91. 19) whereas the lowest was recorded by BJC-5105 (42.79). The largest area of the pyramid was recorded by the accession C-5036 (46.85 sq. mm) followed by the accession C-5044 (41.17 sq. mm) whereas the lowest was showed by BJC-5105 (14.50 sq. mm). These findings are following [17,8,20,6].
The considered traits studied in the present investigation showed high heritability ranging from 92.34% to 97.95%. Among the traits studied the highest heritability was recorded by Area of pyramid (97.95%) followed by the number of fibre bundle (96.69%) and lowest heritability values was recorded by no of layer (92.34%) ( Table 3).
In the present study, the mean of Bark thickness ( 1.59mm), Number of pyramid (6.30), Number of layer (8.28), Number of fibre (71.670), Bundle and Area of pyramid (28.64 sq.mm) was calculated. Genetic advance was the highest for the number of the Number of fibre (23.14) followed by Area of pyramid (19.92 and the lowest for Bark thickness (0.77 mm) among the yield contributing traits ( Table 3). The genetic advance as percentage of mean was the highest for Area of pyramid (70.51 sq. mm) followed by Bark thickness (48.35mm) and the lowest for the number of pyramid (23.03) ( Table 3). [15] Suggested that heritability and genetic advance should always be considered jointly during selection of a suitable line or progeny. The GCV and PCV were found to differ significantly for all the fibre yield components and biotic stress factors, which indicated a major role played by the environment in the expression of these characters. This is in agreement with the findings of [22].
Relationships among different anatomical characteristics were studied through analysis of correlation among them. The correlation co-efficient among different anatomical traits of 40 white jute genotypes are presented in Table 4.
Significantly genotypic correlation was recorded for bark thickness with number of layer, number of fibre bundle and area of pyramid. Besides, Number of layer was significantly correlated with both number of fibre bundle and area of pyramid. Number of fibre bundle showed correlation significantly only with area of pyramid. On the other hand, Number of pyramid is only the parameter which is non significant with all anatomical characteristics and bark thickness was non-significant with number of pyramid. These findings are in accordance with [23,8,20,21].
Depending upon the range of diversity, 28 genotypes were grouped into five clusters ( Table 5 Figure 1). The distribution pattern revealed maximum number of genotypes (18 genotypes)  were in cluster IV covering 39.29% of the total studied genotypes while cluster V included minimum number of genotypes (3 genotypes each). Cluster I and II included 6 genotypes each ( Table 5) A similar of clustering of tossa jute germplasm was observed by [24,25] in tossa jute genotypes.
Mean performance of different clusters for different morphological traits studied are shown in  [25,26] in tossa jute genotypes.

CONFLICT OF INTERESTS
The authors have not declared any conflict of interests.

ACKNOWLEDGEMENT
We are grateful to the genetic resources and seed division, Bangladesh jute research institute, Manik Mia Avenue, Dhaka -1207, Bangladesh for the necessary facilities and encouragement.