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A Method for Isolating Ions Using an Excitation Signal Generated by Superposing and Modulation Frequency

Tandem mass spectrometry (MS/MS) is widely used in the fields of biology and chemistry because it allows for identification and structural characterization of macromolecule. The core process of MS/MS is ion isolation. An ideal ion selection method can lead to accurate analysis results. This paper proposes an ion selection method based on superposition sinusoidal modulated signals. This method is more flexible than the ion selection method based on mixed sinusoidal modulated signal. In addition, the isolation signal generation workflow of this method is also simpler than the short-time storage inverse Fourier transform (SWIFT). Based on the simulation results, we discuss the effect of bandwidth size on ions excitation. The ion resolution of this method is also discussed. Finally, we demonstrate a simulation experiment of ion selection, which proves that this method has the ability to select multiple ions at the same time.