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The isolation and maintenance of the human pilosebaceous unit

We have previously developed methods for the isolation and maintenance of human sebaceous glands and hair follicles. However, in long‐term culture the maintenance of both is suboptimal. This may be due to a lack of stem cells, which are thought to be located in the bulge area of the hair follicle, and this region is not present in either model. Isolation of the entire pilosebaceous unit would retain this region, and may lead to improved maintenance of both structures. We describe a method for the isolation of viable, individual, pilosebaceous units by microdissection from human scalp face‐lift skin. The viability of isolated pilosebaceous units has been determined by light microscopy, patterns of DNA synthesis by [methyl‐3H] thymidine autoradiography, and lipogenesis by [U‐14C] acetate uptake into lipids.