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Effect of placental extracts on the growth of fibroblasts in vitro

Monolayer cultures of human fibroblasts were used both to confirm clinical experiments in which it was found that placental extracts increased the rate of healing and to demonstrate the action on cellular regeneration. The cytotoxic dose of lyophilized extracts from human placentae was first determined and the extracts were then added to culture media at concentrations of 100 to 400 μg/ml.