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Amniotic Fluid Phospholipid Determinations by Bubble Stability Test and Quantitative Lipid Analysis

Abstract. Amniotic fluid surfactant content has been determined in 60 samples obtained throughout the last trimester, by the bubble stability test (BST) described by Clements et al. (3), and studied quantitatively by measuring lecithin and sphingomyelin. Forty samples obtained within one week of delivery were also studied in relation to gestational age and development of the respiratory distress syndrome (RDS). Bubble stability is shown to be dependent on the lecithin concentration. Respiratory distress is shown to be associated with low levels of lecithin and a negative BST, although false negative BSTs may occur.