{'fraction_non_alphanumeric': 0.0400443178220956, 'fraction_numerical': 0.025746544265062784, 'mean_word_length': 5.272336201191264, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 1, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Obesity: Interactions of Genome and Nutrients Intake

Obesity has become one of the major public health problems all over the world. Recent novel eras of research are opening for the effective management of obesity though gene and nutrient intake interactions because the causes of obesity are complex and multifactorial. Through GWASs (genome-wide association studies) and genetic variations (SNPs, single nucleotide polymorphisms), as the genetic factors are likely to determine individuals’ obesity predisposition. The understanding of genetic approaches in nutritional sciences is referred as “nutrigenomics”. Nutrigenomics explores the interaction between genetic factors and dietary nutrient intake on various disease phenotypes such as obesity. Therefore, this novel approach might suggest a solution for the effective prevention and treatment of obesity through individual genetic profiles and help improve health conditions.


INTRODUCTION
The increasing prevalence of obesity is a dramatic public health burden (1); simply, individuals' health problems are associated with a great number of chronic diseases (2). The effects of dietary factors (for example, dietary energy, fat or carbohydrate) on obesity have been reported (3,4). Obesity is the consequence of higher dietary energy intake and lower energy expenditure, which results in an imbalance of energy and an increase in body weight (5). However, obesity is influenced by many other factors such as environmental, behavioral, hormonal, metabolic, and genetic predisposition (6)(7)(8)(9)(10). Recent researches (11)(12)(13)(14) have suggested that genes, environmental factors such as dietary nutrients intake, and their interactions affect obesity. This is important to better understand obesity by individuals' genetic predisposition and to create a concept of "personalized nutrition" for the effective prevention and treatment of obesity.

FACTS ON OBESITY
Obesity is one of the major health concerns that pose a considerable burden to public health all over the world (1). It has been strongly associated with an increased risk of cardiovascular diseases, type 2 diabetes, metabol-ic syndrome, and some types of cancer (2). Globally, the body-mass index (BMI, the weight in kilograms divided by the square of the height in meters) of overweight adults equal to or more than 25 was estimated to be 1.5 billion and obese adults with BMI of 30 or greater were about 500 million in 2008 (15). The fifth Korea National Health and Nutrition Examination Survey (KNHANES V-3) reported in 2012, announced that the overall prevalence of obesity (BMI≥25 kg/m 2 ) in adults is 32.8% (36.1% in men and 29.7% in women) (16).
The primary cause of obesity has been known as an accumulation of excessive body fat resulting from an imbalance of energy intake over physical activity (5). Many studies have reported that high dietary energy is the major contributor to obesity. The intakes of high energy have been associated with consumption of food groups beyond recommended amounts of protein, carbohydrate and fat (13,14). Also, obesity can result from higher consumptions of dietary fat and empty calorie foods, defined as the sum of energy from solid fats and added sugars (17). On the other hand, some studies have shown that diets or foods with high appetite-controlling characters (such as vegetables, fruits, and whole grains) were inversely related to the prevalence of obesity (18,19) This is likely due to the diluted energy density of the diet, the incomplete absorption, and an increase of satiety through delayed gastric emptying of the ingested food. In addition, diet patterns that disturb the regulation of dietary energy intakes, such as skipping a meal, snacking, and away-from-home meals, were also associated with obesity (18). However, low-energy diets with dietary fat restrictions were shown to be unsustainable in maintaining long-term weight loss in obese individuals (20). The role of fruit and vegetable consumption in body weight, as an indicator of obesity, was not clear (21). Intriguingly, the effect of composition or kind of diet on obesity was differently responded by individuals. For example, some individuals were hyper-responsive to beneficial diets (such as diets or foods with high appetite-controlling characters, low-energy diets with dietary fat restrictions) and were maintaining healthy body weights. Others groups were more prone to gain weight to similar diets (19).
Multiple studies of families, adoptions, and twins suggest that genetic factors accounted for 45 to 75% of the inter-individual variations in BMI, the commonly used measure of adiposity (6). In the Swedish twins study, within-pair correlations for BMI were 0.70 for men and 0.66 for women among identical twins reared apart (7). In Korea, heritability of BMI was 82% for men and 87% for women in adolescent twins (8) and heritability of body fat mass was 45% in a strong familial aggregation (9). With the completion of the Human Genome Project in 2003, the single nuleotide polymorphisms (SNPs), which constitute the most frequent DNA sequence variants in the genome were identified (10).

SNPs AS A MAIN FORM OF GENETIC VARIATIONS
As a main form of human genetic variations, SNPs are single nucleotides-A (adenine), T (thymine), C (cytosine), or G (guanine)-differences in DNA sequences both within and among populations (22). For example, two sequenced DNA fragments from different individuals, CCTAC to CTTAC (forward; GGATG to GAATG as reverse), contain a difference in a single nucleotide (Fig.  1). These C and T are called alleles. Almost all common SNPs have only two alleles (biallelic); one for each of the 22 autosomal chromosomes inherited independently from his or her parents. SNPs, as distinguished from the term "mutation (＜1% in the human population)", exist at a frequency of 1% or higher in the human population (23).
The SNPs occur throughout the genome, on average every 300 base pairs (24). If two humans are selected at random, the difference in SNPs between them will be around 10 million nucleotide differences because all humans have almost the same sequence of 3 billion nucleotides distributed between their 23 pairs of chromosomes (3×10 9 base pair÷300 base pair≒10×10 6 SNPs).
A genotype is an observed set of particular alleles at specified loci (25). In the former example ( Fig. 1), there are 3 potential genotypes: C/C, C/T, and T/T; the genotypes of individuals with the C/C or T/T alleles and that of individuals with C/T are assigned as being homozygous and heterozygous, respectively. The expression of genotype results in the individual's observable traits, the phenotype. Also, the differences of genotype have certain functional consequences, such as altering the activity/function of a protein, and it is followed by the cause of common diseases (22). In other words, the differences in SNPs between individuals may explain why some individuals are more susceptible to common diseases.

GENOME-WIDE ASSOCIATION STUDIES (GWASs) IN OBESITY
The GWASs have become a common method for examining genetic variations, such as finding heritable risk factors associated with a particular complex disease (26,27). The GWASs are based on the "common disease, common variant" hypothesis, which suggests that common allelic variants present in more than 1% to 5% of the population are the major contributors to genetic susceptibility towards diseases (28,29). The entire human genome was scanned by a high-throughput approach, which helped GWASs to determine associations between chromosomal loci and given diseases genome in an unbiased manner (30). The GWASs have been per- formed for several complex diseases including type I and type II diabetes, inflammatory bowel disease, prostate cancer, breast cancer, asthma, and coronary artery disease (27). Novel loci contributing to BMI and obesity have also been found (14,31,32). The results from such studies have demonstrated that genetic variations could provide valuable insights for several diseases as well as obesity.
The GWASs genotyped by DNA microarrays are commonly called DNA SNP chips (8,33). The DNA microarrays, which were initially developed in the 1990s, are short DNA probes attached to small glass slides. When fluorescent-labeled DNA fragments of a sample are added to the microarray, they are hybridized to the chip and can then be detected by scanning software (Fig. 2).
The first GWAS for obesity and related traits identified a variant 10 kb upstream of INSIG2 (insulin-induced gene-2) in the Framingham Heart Study (34). However, the results from subsequent studies have been inconsistent (35)(36)(37). Afterward, FTO (fat mass and obesity associated gene), an obesity susceptibility locus identified, has remained the initial gene to be regarded as a well established and thoroughly replicated risk factor for common obesity (38,39). The FTO is a locus with the largest effect on BMI, so its locus is recognized as a powerful genetic susceptibility locus for obesity. In a metaanalysis data of 7 GWASs for obesity and related traits in 16,876 individuals of which 11,012 were from 4 European population-based cohorts, and 5,864 from 3 disease-specific case-series, a variant mapped 188 kb downstream of MC4R (melanocortin-4 receptor) was identified to play a role in the monogenic forms of obesity (40). Besides the FTO and near-MC4R loci, the three other GWASs in 2009 from the GIANT (genomic investigation of anthropometric traits) consortium (41), deCODE genetics (42), and GWAS in European populations (43) identified 13 new loci, near NEGR1 (neuronal growth regulator 1), near TMEM18 (transmembrane protein 18), in SH2B1 (SH2B adaptor protein 1), near KCTD15 (potassium channel tetramerization domain containing 15), near GNPDA2 (glucosamine-6-phosphate deaminase 2), in MTCH2 (mitochondrial carrier homologue 2), in SEC16B (SEC16 homologue B), between ETV5 (ets variant gene 5) and DGKG (diacylglycerol kinase), in BDNF (brain-derived neurotrophic factor), between BCDIN3D (BCDIN3 domain containing) and FAIM2 (Fas apoptotic inhibitory molecule 2), in NPC1 (Niemann-Pick disease, type C1), near MAF (v-maf musculoaponeurotic fibrosarcoma oncogene homologue), and near PTER (phosphotriesterase related).

DIET AND GENETIC VARIATION ON OBESITY
In spite of a number of recent studies in identifying genetic variants on obesity using the GWASs, it is well established that solely those variants do not result in obesity without the exposure of an obesogenic environment. Also, an interaction between genetic and environmental factors on obesity might exceed the effect of specific genetic variants (11). In other words, obesity should be considered as a complex multifactoral disease affected by genetic factors as well as environmental influences, such as diet and physical activity (12)(13)(14). The genetic factors and genetic variations (SNPs) are likely to determine an individual's susceptibility to obesity through the full series of potential mechanisms governing pathways and regulatory systems at different levels, including the intake and expenditure of energy and the controlling and partitioning of nutrients between fat and lean mass tissue.
The understanding of genomic approaches in nutritional sciences has created a new field, called "nutrigenomics" (44)(45)(46)(47). Nutrigenomics consists of nutrigenomics and nutrigenetics, which explores the interaction between nutrients and genes. However, it is distinctly discriminated through mechanisms of how interactions between nutrients and genes influence the risk for devel- Fig. 3. The understanding of genomic approaches in nutritional science has created a new field, called "nutrigenomics". Nutrigenomics consists of nutrigenomics and nutrigenetics: nutrigenomics is the effect of nutrients or nutritional supplements on gene expression, and nutrigenetics is the effect of interaction between genetic variations and nutrients on diseases. oping a health condition or disease (Fig. 3) (44,45). Nutrigenomics describes the effect of nutrients (foodbased diets, dietary restriction, or nutritional supplements) on gene expression; it will increase interest in the genome-wide influences of nutrients on transcriptome, proteome, and metabolome in cells, tissues, or organisms, as well as confirm the genes that influence the risk of diet-related diseases (46). It may also be helpful in the understanding of how nutrients influence metabolic pathways and how these regulations are inhibited in the early phase of a diet-related disease (44).
In contrast, nutrigenetics is focused on how the interaction between genetic variations and nutrients influence metabolism, health conditions, and the risk for diet-related diseases (44,47). In other words, the influence of nutrients on diseases is determined by an individual's genetic variations, which can affect digestion, absorption, metabolism, partitioning, and cellular responsiveness to nutrients (47). Nutrigenetics is useful in providing genetic profiles for the early detection of disease risks and may provide clues for personalized diet recommendations for effective prevention strategies or therapies for individual with genetic predispositions to diseases ( Fig. 4) (47). This approach, along with individuals' motivation to adapt lifestyle changes, may provide health benefits to individuals.

STUDIES OF INTERACTIONS BETWEEN DIET AND SNPs ON OBESITY
Several studies examined the interactions between dietary nutrient intake and genetic variations (SNPs) involved in obesity related variables. Sonestedt et al. (48) reported interactions between energy-adjusted fat (P= 0.04) or carbohydrate intake (P=0.001) and a variant of the FTO gene (rs9939609) on BMI among 4,839 subjects in a cross-sectional study; among the participants with an intake of high fat or low carbohydrate, the AA carrier showed a higher BMI than the TT carrier of FTO rs9939609. In the GOLDN (Genetics of Lipid Lowering Drugs and Diet Network) study, Warodomwichit et al. (49) found that subjects who were carriers of the ADIPOQ (adiponectin, C1Q and collagen domain containing)-11391A allele (AA+GA) had significantly lower body weight (P=0.029), BMI (P=0.019), waist circumference (P=0.003), and hip circumference (P=0.004) compared to noncarriers. In addition, in subjects with high monounsaturated fatty acids intake, carrier of the -11391A allele resulted in lower BMI (P=0.002) and decreased risk of obesity (odd ratios=0.52, 95% CI=0.28 ∼0.96, P=0.031). Also, it was reported that gene-dietary fat interaction might modulate the risk of obesity. The APO B (apolipoprotein B) SNP rs512535 (A/G) minor allele carrier (G) has been associated with obesityrelated phenotypes (BMI and waist circumference). Interestingly, in habitual high fat consumers (＞35% of energy), the GG homozygotes of APO B rs512535 have shown higher BMI than the A allele carriers, however those differences were not shown in low fat consumers (＜35% of energy) (50).
Recently, several studies have been conducted on the various roles of obesity-related genotype in Korea. We previously observed that the ESR1 (estrogen receptor 1) rs1884051 polymorphism (C＞T) was associated with obesity-related variables (body weight, BMI, waist-hip ratio, fat body mass, and body fat percentage), together with their modulations by dietary intake in 3,039 Korean men aged 40∼59 years from the KoGES database (51). Moreover, among the subjects with low total energy intake, the minor allele of ESR1 SNP resulted in a lower BMI (P=0.003) compared to the subjects carrying the major allele. Interestingly, among subjects with a high plant protein intake, carriers of the minor allele of ESR1 SNP had a lower BMI (P=0.044) compared to subjects carrying the major allele. Cha et al. (52) reported that the increased BMI was associated with the FTO haplotypes and MC4R variants in two populations using 1,370 Korean subjects before and after Sasang constitutional medicine (SCM) typing, and found the BMI in 538 individuals lowering with a lifestyle intervention for one month. After lifestyle changes such as a low-calorie diet, daily exercise, an electrolipolysis treatment, and the administration of Chegamuiyiin-tang containing 17 herbs, carriers of the haplotype by the minor allele of rs1075440 had a lower waist-to-hip ratio (0.76%) compared to the non-carriers. The rs9939973 and rs9939609 of FTO were recently shown to modulate obesity in 711 Korean children and 8,842 adults. A significant association was identified between rs9939609 and dietary fat intake in children (P=0.008) but not in adults (53). Among the 6 SNPs of WNT10 B gene, known as a potential regulator of adipogenesis in obesity models, -607G＞C (rs833840) was significantly associated with body fat mass by bio-impedance analysis and abdominal fat (total and subcutaneous fat areas) by abdominal computed tomography in 1,029 Korean women. However, no significant associations between -607G＞C genotype and body weight changes or composition were confirmed among 576 subjects with very low calorie diet for one month (54).

CONCLUSION
The cause of obesity is the complex impact of genetic and environmental factors. It is associated with increased risks of developing many chronic disease including cardiovascular disease, type 2 diabetes, arthritis, hypertension, and certain cancers such as esophagus, breast, endometrium, colorectal, gallbladder and possibly other types of cancers. As dietary nutrient intake is an important environmental factor, many studies have shown that dietary intake plays a key role in the development of the obesity. Although many studies have observed the effects of quantity and quality of dietary nutrients on obesity, intervention studies are inconsistent.
Which factors make a difference on obesity with the same dietary intake in individuals? The answer is the difference in genetic variation. In other words, the features of nutrigenomics are explained by the relationship between specific dietary nutrient intake and gene varia-tions on obesity. Now, nutrigenomics is extensively used for researching obesity as well as diet-related disorders. Therefore, nutrigenomics may shift towards the effective management of obesity, through "personalized" nutritional advice and consultation by individuals' genetic profiles.
Even though many genetic variations to affect the development of obesity are identified in all over world, the unique differences in the genetic variations on obesity by races or populations exist. So, further investigations are still necessary in various genetic variations on obesity, association between various dietary nutrients intake and genetic variation on obesity for effective management tools of personalized nutrition on obesity for various race or populations. And those investigations may provide a solution to a public health problem, as well as personalized obesity prevention.