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Fiber coupling of an infrared femtosecond laser to a multiphoton microscope

We report on a new fiber delivery system for coupling a compact, turn-key infrared femtosecond laser into a multiphoton microscope. Using a hollow core photonics crystal fiber with minimized dispersion, we achieve beam propagation on distances up to 4 meters. We measured a pulse duration close to 300 fs at the output of the microscope, for a femtosecond laser pulse duration of 200 fs. We present experimental parameters and system optimisation, and compare images obtained with fiber coupling versus direct coupling of the femtosecond laser.