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Time-resolved multi-dimensional fluorescence imaging using a Digital-Micromirror-Device and a SPAD-array detector

A widefield system for multidimensional fluorescence imaging capable of resolving space, time and wavelength is developed and validated on a synthetic fluorescence sample. The system enables structured illumination and compressing detection. A compression strategy based on an a-priori information obtained by a camera is validated and proved to be effective at compression ratio of about 90%.