{'fraction_non_alphanumeric': 0.025365853658536587, 'fraction_numerical': 0.013073170731707317, 'mean_word_length': 5.175903614457831, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Seroprevalence of 2 zoonotic diseases in Southwestern Saudi Arabia. Rift Valley fever and brucellosis.

R Valley fever (RVF) is an arthropod-borne viral disease characterized by high mortality rates in young animals and abortions in pregnant ruminants.1 The virus is transmitted to humans through mosquito bites, or by exposure to blood and bodily fluids.1,2 Epidemics of RVF were limited to the African continent until the year 2000, when an epidemic occurred simultaneously in southwestern Saudi Arabia, and the neighboring north-western regions in Yemen.3 Even though no clinical cases of RVF among humans were reported from regions outside the epicenters in the Kingdom of Saudi Arabia (KSA), there is always a concern on the possibility of spread of this infection to other regions.3 Brucellosis is an infectious disease caused by bacteria of the genus Brucella that cause significant economic losses for animal owners, and severe human disease.4,5 Brucella can survive for long periods in dust, dung, water, aborted fetuses, soil, meat, and dairy products. As the infection dose is very low, infections are an occupational risk for farmers, veterinarians, abattoir workers, laboratory personnel, and others who work with animals, and consume their products. Infection is transmitted to humans through direct contact with the infected animals, or by consuming infected milk or fresh cheese, and undercooked meat products.4,5 Continuous monitoring programs allow the evaluation of the current status of RVF and brucellosis’ prevalence, and help in the effectiveness of control measures. Although many studies have been carried out on RVF2,3 and brucellosis4,5 in KSA, there is a clear need for new studies on the seroprevalence of these diseases in Najran region. The objective of this work is to investigate the prevalence of RVF and brucellosis in Najran region. Blood samples were collected from humans and animals in Najran region from September 2009 to June 2010. The human blood samples were collected from 2 groups. The first group consists of 540 apparently healthy people with a high-risk of contracting brucellosis (farms and abattoir workers). Informed consent was obtained from the participants. A 5 ml sample of peripheral venous blood was collected from subjects at their place of employment. In the second group, blood samples were collected from 900 patients, suffering from fever, of different ages and gender at King Khalid Hospital in Najran. The animal samples (sheep and goats) were collected from the 2 groups: first group 640 sheeps and 320 goats from different unvaccinated flocks with a history of abortions; and the second group 450 blood samples were collected from healthy slaughtered sheep (n=300) and goats (n=150) from the Najran abattoir. Blood samples were transported to the laboratory on ice, where they were centrifuged, and the sera were separated. Humans and animals sera were tested by indirect enzyme-linked immunosorbent assay (ELISA [Biological Diagnostic Supplies Limited, UK]) for immunoglobulin (Ig) M, and IgG antibodies against RVF virus. Human sera were tested by indirect ELISA (Laboratorios Vircell, Granada, Spain) for IgM and IgG antibodies against brucellosis. Animals sera were tested by slide agglutination test (SAT) for antibodies using stained bacterial antigen (Omega Diagnostic Ltd Carsbridge Court, Alloa, Scotland, UK), and the Brucella was considered positive when a titer of ≥1:160 was obtained. All tests were carried out at Biosafety Level 3 at Najran University, Najran, KSA, and were performed according to the manufacturer’s instructions. This study was approved by the Research and Ethics Committee of the College of Medicine, Najran University, Najran, KSA. All human and animal tested samples were negative for anti-RVF virus IgM and IgG antibodies. For brucellosis, the results of ELISA revealed that all apparently healthy people samples were negative for IgM antibodies, while 12 were positive for IgG antibodies. Thirty of the feverish patient’s sample were positive for IgM antibodies, while 66 were positive for IgG antibodies. The results of SAT revealed that 96 of diseased sheep samples, 46 of diseased goat samples, and 2.7% of healthy slaughtered sheep and goat samples were positive against Brucella melitensis antibodies. Table 1 summarizes the results of brucellosis test. Rift Valley fever virus can establish itself wherever potential permissive vectors and animal reservoirs are present. The presence of competent vectors in regions previously free of RVF, the high viral titers in viremic animals, as well as the global changes in climate, travel and trade all contribute to make this virus a threat that must not be neglected.1 Najran region is a rural area where the main occupations of the population include animal husbandry, and the climate favors breeding of mosquitoes. The results showed that all humans and animals tested samples were negative against RVF IgG and IgM antibodies. Our results agree with many studies performed in different localities in KSA, and indicated that the disease is still localized in the area of