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In Vitro Induction of Crown Galls by Agrobacterium Tumefaciens Super Virulent Strain A281 (pTiBo 542) in Salvia Sclarea and S. Pratense

ABSTRACT Salvia sclareae and S. pratense are important medicinal plants. The application of genetic engineering to these important crops should allow incorporation of some traits like insect and herbicide resistance. Co-cultivation experiments with A281 strain of Agrobacterium tumefaciens using leaf explants excised from 35 days old seedlings of both species showed that these were highly susceptible to transformation. Moreover, the strain A281 resulted in development of shooty tumors in S. sclarea and rooty tumors in S. pratense, which were selected on MS medium containing 50 mg/1 kanamycin. All of them were GUS positive.