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Measurement of basal, substrate induced and total P‐glycoprotein activity in bronchoalveolar lavage T‐cell subsets

P‐glycoprotein (P‐gp) is a member of the ABC transporter superfamily. P‐gp activity can be detected by measuring efflux of fluorescent substrates such as rhodamine 123 (R123). Our objectives were to evaluate P‐gp activity in T cells freshly isolated from bronchoalveolar lavage (BAL) and to develop a strategy to distinguish between basal, in vitro substrate‐induced, and total P‐gp activities.