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Crystal structure and functional characterization of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea strain 34H

Isoaspartyl dipeptidase (IadA) is an enzyme that catalyzes the hydrolysis of an isoaspartyl dipeptide-like moiety, which can be inappropriately formed in proteins, between the β-carboxyl group side chain of Asp and the amino group of the following amino acid. Here, we have determined the structures of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea, both ligand-free and that complexed with β-isoaspartyl lysine, at 1.85-Å and 2.33-Å resolution, respectively. In both structures, CpsIadA formed an octamer with two Zn ions in the active site. A structural comparison with Escherichia coli isoaspartyl dipeptidase (EcoIadA) revealed a major difference in the structure of the active site. For metal ion coordination, CpsIadA has a Glu166 residue in the active site, whereas EcoIadA has a post-translationally carbamylated-lysine 162 residue. Site-directed mutagenesis studies confirmed that the Glu166 residue is critical for CpsIadA enzymatic activity. This residue substitution from lysine to glutamate induces the protrusion of the β12-α8 loop into the active site to compensate for the loss of length of the side chain. In addition, the α3-β9 loop of CpsIadA adopts a different conformation compared to EcoIadA, which induces a change in the structure of the substrate-binding pocket. Despite CpsIadA having a different active-site residue composition and substrate-binding pocket, there is only a slight difference in CpsIadA substrate specificity compared with EcoIadA. Comparative sequence analysis classified IadA-containing bacteria and archaea into two groups based on the active-site residue composition, with Type I IadAs having a glutamate residue and Type II IadAs having a carbamylated-lysine residue. CpsIadA has maximal activity at pH 8–8.5 and 45°C, and was completely inactivated at 60°C. Despite being isolated from a psychrophilic bacteria, CpsIadA is thermostable probably owing to its octameric structure. This is the first conclusive description of the structure and properties of a Type I IadA.

Isoaspartyl dipeptidase (IadA) is an enzyme that catalyzes the hydrolysis of an isoaspartyl dipeptide-like moiety, which can be inappropriately formed in proteins, between the β-carboxyl group side chain of Asp and the amino group of the following amino acid. Here, we have determined the structures of an isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea, both ligand-free and that complexed with β-isoaspartyl lysine, at 1.85-Å and 2.33-Å resolution, respectively. In both structures, CpsIadA formed an octamer with two Zn ions in the active site. A structural comparison with Escherichia coli isoaspartyl dipeptidase (EcoIadA) revealed a major difference in the structure of the active site. For metal ion coordination, CpsIadA has a Glu166 residue in the active site, whereas EcoIadA has a post-translationally carbamylated-lysine 162 residue. Site-directed mutagenesis studies confirmed that the Glu166 residue is critical for CpsIadA enzymatic activity. This residue substitution from lysine to glutamate induces the protrusion of the β12-α8 loop into the active site to compensate for the loss of length of the side chain. In addition, the α3-β9 loop of CpsIadA adopts a different conformation compared to EcoIadA, which induces a change in the structure of the substrate-binding pocket. Despite CpsIadA having a different active-site residue composition and substrate-binding pocket, there is only a slight difference in CpsIadA substrate specificity compared with EcoIadA. Comparative sequence analysis classified IadA-containing bacteria and archaea into two groups based on the active-site residue composition, with Type I IadAs having a glutamate residue and Type II IadAs having a carbamylated-lysine residue. CpsIadA has maximal activity at pH 8-8.5 and 45˚C, and was completely inactivated at 60˚C. Despite being isolated from a psychrophilic bacteria, CpsIadA is thermostable probably owing to its octameric structure. This is the first conclusive description of the structure and properties of a Type I IadA. PLOS

Introduction
Abnormally modified proteins containing a β-linked Asp residue (isoaspartyl linkage) are not allowed to accumulate in cells because the formation of isoaspartyl residues may induce the loss of protein function. Isoaspartyl-modified polypeptides result from chemical reactions such as the deamidation of asparagine or the dehydration of aspartate [1][2][3]. Bacteria contain three different enzyme recovery mechanisms that can repair isoaspartyl modified proteins. The first enzyme is protein-L-isoaspartyl O-methyltransferase (PIMT) [4][5][6]. PIMT repairs the damaged intermediate by transferring a methyl group from S-adenosyl-L-methionine to the α-carboxylate side chain of the isoaspartyl residue. The second enzyme is isoaspartyl aminopeptidase (IaaA) [7][8][9]. IaaA can hydrolyze the iosaspartyl peptide and has L-asparaginase activity. The third enzyme is isoaspartyl dipeptidase (IadA) [10,11]. IadA is a binuclear metalloenzyme and a member of the amidohydrolase superfamily. This enzyme catalyzes the hydrolytic cleavage of β-aspartyl dipeptides. Thus, only IaaA and IadA can degrade isoaspartyl modified proteins because most proteases and peptidases do not recognize β-linked Asp residues [12,13]. Without a specific dipeptidase, isoaspartyl dipeptides might accumulate and could be toxic to the cell. In previous studies, the crystal structures of IadA from Escherichia coli (EcoIadA) have been solved both in its ligand-free state and complexed with aspartate, as well as with a transition state analog [the phosphinic inhibitor, Asp-psi(PO2CH2)-Leu-OH] and β-Asp-His [14][15][16]. These structural studies have demonstrated that the active site of EcoIadA contains two Zn ions, which are involved in the catalytic reaction. Structural information studies have also revealed conserved key residues in the catalytic site that constitute the metal and substrate binding sites. It is interesting to note that EcoIadA has a carbamylated lysine residue (K162) in its active site and this residue is important for metal ion coordination. The carbamylation on lysine is a post-translational modification that changes the charge on the lysine side chain from a positive to a negative charge and also extends the residue length by about 2 Å to allow for metal coordination. Further biochemical studies have provided insight into the substrate specificity of EcoIadA [14]. The results have shown that EcoIadA has a strong preference for β-Asp-Leu and β-Asp-Phe.
In the present study, we determined the crystal structures of IadA from Colwellia psychrerythraea strain 34H (CpsIadA) in its ligand-free form and that in complex with β-isoaspartyl lysine, and subsequently characterized its enzymatic properties. Colwellia psychrerythraea 34H is a strictly psychrophilic bacteria found in Arctic marine sediment and its full genome sequence information has been reported [17]. Thus, several proteins from the bacteria have been studied to examine cold-adapted activity and cold-active structural properties compared with mesophilic bacterial proteins. The most notable feature of CpsIadA is the different residue configurations in the active site compared with EcoIadA. In CpsIadA, the Glu166 residue corresponds in position to the carbamylated lysine 162 residue in EcoIadA. A comparison of the structures, together with extensive sequence alignment, indicated that IadAs can be classified into two groups, depending on the configuration of active site residues, as represented by EcoIadA and CpsIadA. In addition, the β-isoaspartyl lysine-complexed CpsIadA structure provided details of the interaction between β-isoaspartyl lysine and CpsIadA, as well as information about the conformational changes induced by substrate binding. Furthermore, biochemical analysis, together with the structural information and site-directed mutagenesis, also provided useful insights in understanding the substrate specificity of CpsIadA and as well as identifying residues important for enzymatic catalysis.

Crystallization and data collection
Purified CpsIadA and CpsIadA E80Q mutant proteins were concentrated to 35 mg/mL and 54 mg/mL respectively. For determination of the enzyme-substrate complex, β-aspartyl lysine (5 mM) was added to the CpsIadA E80Q mutant protein solution and incubated for two hours at room temperature before crystallization. Initial screening of crystallization conditions was carried using a crystallization Mosquito robot (TTP Labtech, Cambridge, MA, USA) with the hanging-drop vapor-diffusion method at 293 K in 96-well crystallization plates (Emerald Bio, Bainbridge Island, WA,USA). The drops contained 0.6 μL of protein solution and 0.6 μL of reservoir solution and were equilibrated against 300 μL of reservoir solution. The successful crystallization conditions for CpsIadA consisted of 0.1 M Bis-Tris propane pH 7.0, 1.4 M sodium malonate (SaltRx #C11). The best crystals for the β-aspartyl lysine complexed CpsIadA E80Q mutant appeared in 0.1 M Bis-Tris propane pH 7.0, 1 M ammonium citrate tribasic pH 7.0, (MCSG3 #C7). The crystals obtained were protected from the liquid-nitrogen gas stream using Paratone-N oil (Hampton Research, Aliso Viejo, CA, USA).
X-ray diffraction data were collected using the BL-5C beam line of the Pohang Accelerator Laboratory (PAL; Pohang, Korea). At a resolution of 1.85 Å, the data set for ligand-free wild type CpsIadA contained 100 images with 1˚oscillation and an exposure time of 1 s per image and at a resolution of 2.33 Å the data set for β-aspartyl lysine complexed CpsIadA E80Q mutant contained 100 images with 1˚oscillation and an exposure time of 1 s per image. The data sets were indexed, processed and scaled using HKL-2000 program [18]. The statistics for the X-ray diffraction data sets are shown in Table 1.

Structure determination and refinement
The crystal structure of the ligand-free CpsIadA was solved by molecular replacement, using the MOLREP program from the CCP4 suite, with the crystal structure of IadA from E. coli (PDB code 1ONW; sequence identity, 43%) as the search model [15,19]. The Matthew coefficient of 2.67 Å 3 Da −1 suggests that two monomers are present in the asymmetric unit [20]. The model was rebuilt using Coot and was refined with REFMAC from the CCP4 suite and the PHENIX program [21][22][23][24]. The final model of ligand-free CpsIadA had an R work and an R free of 16.3% and 20.1%, respectively. Molecular replacement for the β-aspartyl lysine complexed CpsIadA E80Q mutant was conducted using the MOLREP program from the CCP4 suite with the final refined crystal structure of ligand-free CpsIadA as the search model. Successive rebuild and refinement were performed using the REFMAC program from the CCP4 suite and the PHENIX program. The chemical coordination file for β-aspartyl lysine was built using Coot and eLBOW [25]. The final model of the β-aspartyl lysine complexed CpsIadA E80Q mutant had an R work and an R free of 18.9% and 24.6%, respectively. The qualities of the final structural models were checked using MolProbity [26]. The detailed refinement statistics are listed in

Analytical ultracentrifugation
Sedimentation velocity analysis of CpsIadA was performed at 20˚C with a XL-A analytical ultracentrifuge (Beckman Coulter, Brea, CA, USA). The protein solution (0.5 mg/mL) was dissolved in a buffer of 25 mM Tris-HCl (pH 8.0), 200 mM NaCl, 5 mM MgCl 2 and 2 mM dithiothreitol. The sample and reference sectors of the dual-sector epon centerpiece were filled with the CpsIadA protein solution and the buffer, respectively, and the cell was centrifuged at a rotor speed of 45,000 rpm. The sedimentation profile was monitored over time at 280 nm, and the experimental data were analyzed using the SEDFIT program [27,28].

Enzyme activity assay
The activity of CpsIadA was assayed using a continuous spectrophotometric coupled enzyme assay, as previously described [14]. Aspartyl dipeptides including β-Asp-Leu, β-Asp-Gly, β-Asp-Phe, β-Asp-Lys, and β-Asp-His, and α-Asp-Leu were tested as substrates. The standard reaction was carried out at 30˚C and the reaction mixture contained 100 mM HEPES (pH 8.0), 100 mM KCl, 3.7 mM α-ketoglutarate, 0.4 mM NADH, 0.64 unit of malate dehydrogenase, 6 units of aspartate aminotransferase, and CpsIadA. The reaction was initiated by the addition of 10 mM substrate. The hydrolysis of aspartyl dipeptides was monitored by coupling the formation of aspartate to the oxidation of NADH. The change in the NADH concentration was measured at 340 nm (Multiskan GO, Thermo Scientific, Vantaa, Finland). The activity of CpsIadA toward β-Ala-Ala was assayed using alanine dehydrogenase [14]. The reaction mixture contained 100 mM HEPES (pH 8.0), 1.5 mM p-iodonitrotetrazolium violet (INT), 1.5 mM NAD + , 2.0 units of diaphorase, 7 units of L-alanine dehydrogenase, substrate, and CpsIadA. The conversion of INT from the oxidized to the reduced form at 30˚C was monitored at 500 nm.

Temperature and pH studies
To investigate the effect of reaction temperature on the activity of CpsIadA, reactions were performed at temperatures over a range of 5 to 60˚C under the standard assay conditions, using β-Asp-Leu as a substrate. To evaluate the thermal stability of CpsIadA, the enzyme solution was incubated at 0, 20, 50, 60, and 70˚C for 90 min, and aliquots were taken every 15 min. The residual activities were measured under the standard assay conditions. To determine pH dependence of CpsIadA, reactions were carried out at various pHs ranging from 5.0 to 10.0. The various buffers used were 100 mM sodium acetate (pH 5.0-7.0), 100 mM HEPES (pH 6.5-8.5), and 100 mM Tris HCl (pH 7.0-10.0).

Kinetics
The kinetic parameters of the wild-type enzyme were determined in the same reaction mixtures as described above by varying the concentration of the dipeptide substrate. The data were fit to the Michaelis-Menten equation using a nonlinear regression (GraphPad Prism 5 Software, San Diego, CA, USA).

Circular dichroism
Circular dichroism (CD) measurements were performed using a Chirascan Circular Dichroism Spectropolarimeter (Applied Photophysics, Surrey, UK) equipped with a temperature control system. The CD spectra of samples in a quartz cuvette with a 0.1 cm path length were recorded in the far-UV region (190 to 260 nm). Scans were collected at 0.1-nm intervals with a 1-nm bandwidth. Each spectrum was corrected in triplicate, followed by averaging, and baseline subtraction. The thermal denaturation curve was obtained by plotting changes in the CD values at 222 nm over a temperature range of 5 to 99˚C at intervals of 2˚C. The denaturation temperature (T m ) was defined as the point at which 50% of the sample denatured.

Phylogenetic tree
For phylogenetic analysis of CpsIadA, bacteria and archaea sequences homologous to CpsIadA with at least 40% of amino acid sequence identity were used. The analysis involved a total of thirty-nine amino acid sequences including CpsIadA. Multiple sequence alignment was carried out using Clustal Omega [29] with default parameters. Based on the alignment, a phylogenetic tree was drawn using the Neighbor Joining method in MEGA7 [30].

Results and discussion
Overall structures of ligand-free and β-isoaspartyl lysine-bound CpsIadA The ligand-free crystal structure of isoaspartyl dipeptidase from Colwellia psychrerythraea 34H (CpsIadA) and structure of the CpsIadA E80Q mutant complexed with β-isoaspartyl lysine were determined to resolutions of 1.85 Å and 2.33 Å, respectively (Fig 1 and Table 1). Both structures belong to the P42 1 2 space group and contain two monomers in the asymmetric unit. Those two monomers form a dimer, and four dimers from neighboring the asymmetric unit form a propeller shaped octameric structure. The CpsIadA monomer structure contains ten α-helices and eighteen β-strands, and the overall structure is divided into two distinct domains (a β-sandwich domain and a catalytic domain) (Fig 1A). The β-sandwich domain is composed of eight β-strands (β1, β2, β3, β4, β5, β16, β17, and β18) and this domain contains both the N-and C-termini. The catalytic domain forms an αβ-barrel structure with the eight central β-strands surrounded by the ten α-helices. Two Zn ions are located in the central cavity of the αβ-barrel structure.
Although no exogenous metal ions were added to the CpsIadA protein solution during purification or crystallization, two strong electron densities were identified in the Fo-Fc map. A careful examination of the interactions and comparison with previously determined EcoIadA structure (which contained two Zn ions at corresponding positions) indicated the Crystal structure of isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea strain 34H potential for two divalent metal ions. We performed an X-ray fluorescence scan using the crystal to determine the identity of the metal. The result showed that a strong fluorescence signal was detected near the zinc K absorption edge and that the emission peaks from this scan were unambiguously characteristic for zinc. Therefore, we confirmed the presence of Zn ions. Zinc ions were, therefore, included in the model. After refinement, the B-factors of ZN1 and ZN2 on chain A were 31.90 Å 2 and 24.13 Å 2 , respectively (the overall B-factor of protein residues is 25.3). Moreover, the occupancies of the two Zn ions have been estimated as 1.0 using the Phenix program [24], which further explains their incorporation (and retention) within the protein structure without the addition of exogenous Zn ions (S1 and S2 Figs).
Previous studies have shown that these metal ions are directly involved in the catalytic mechanism of IadA [14,15]. The antiparallel β14 and β15 strands are located near the metal binding site and appear to form the gate for substrate entry or product release because of the relatively high B-factors observed in this region. In the crystal structure of the ligand-free CpsIadA, this part of the β14 and β15 region (residues 297-312) was disordered. In the case of the crystal structure of the CpsIadA E80Q mutant complexed with β-isoaspartyl lysine, this region (residues 301-309) was also partially disordered.
Previously, isoaspartyl dipeptidase from Escherichia coli (EcoIadA) has been shown to be an octamer (tetramer of dimers) [16]. Consistent with this observation, analytical size-exclusion chromatography (SEC) and analytical ultracentrifugation (AUC) of CpsIadA showed that CpsIadA also adopted an octameric state in solution. AUC experiments using 0.5 mg/mL CpsIadA (residues 1-395; calculated molecular weight of 42.7 kDa for the polypeptide chain) gives a mass of 314 kDa (sedimentation coefficient of 13.37 S and a frictional ratio of 1.245), indicating that CpsIadA is a stable octamer in solution.
The CpsIadA monomer forms a dimer via strong hydrophobic interactions between each βsandwich domain. The Trp51 residue located on the β3-β4 loop, protrudes toward the hydrophobic core (Leu8, Leu10, Leu28, Ala35, Ala36, Ile45, Ile47, and Val53; these residues are shown in cyan in Fig 2A of the other monomer. Several other hydrophobic interactions and hydrogen bonds also participate in dimerization (Asn32, Tyr38, Pro48, Pro52, Leu120, Ile147, Phe157, Leu158, and Tyr124; these residues are shown in yellow in Fig 2A. The dimer-dimer interface is formed by two different patches. In the first patch (shown in blue in Fig 2C), the α3 and α4 helices, the α5-α6 loop, and the α7-β12 loop regions interact with the α4 helix, the β6-α1 loop, the β7-α2 loop, the β8-α3 loop, the β10-α5 loop regions of the other dimer. The second interaction patch (shown in red in Fig 2C) is formed by the interactions between the β6-α1 loop, the β7-α2 loop, the α2 helix, the β15 strand and the C-terminal region of the neighboring dimer.

CpsIadA active site
The CpsIadA active site contains two Zn ions (ZN1 and ZN2) with the ions being separated by 3.2 Å in distance. ZN1 interacts with the NE2 atom of His73, the OE2 atom of Glu166, and the OD1 atom of Asp293. ZN2 interacts with the OE1 atom of Glu166, the ND1 atom of His205, and the NE2 atom of His234 (Fig 2B). Notably, Glu166 directly interacts with the Zn ions in the CpsIadA structure. However, in the EcoIadA structure, carbamylated lysine 162 residue is located in this corresponding position and stabilizes the Zn ions. Further sequence alignment revealed that IadA containing bacteria and archaea can be classified into two groups (Type I IadAs having a glutamate and Type II IadAs having a carbamylated-lysine residue) based on the active site residue composition (Fig 1B).
A structural comparison between the ligand-free wild type CpsIadA structure and the structure of the CpsIadA E80Q mutant complexed with β-isoaspartyl lysine (Fig 3A and 3B) show several structural changes occur upon ligand binding. Notably, upon substrate binding, a slight tilt of residue Tyr140 is observed with a 61˚rotation. Tyr140 interacts with the O04 and O07 atoms of the bound β-isoaspartyl lysine (Fig 4A and 4C). The activity assay revealed that the Y140F mutant of CpsIadA had a significant reduction rate of catalysis confirming that this tyrosine residue is important for enzymatic catalysis by CpsIadA. In previous studies, the EcoIadA Y137F mutant (corresponding to Tyr140 in CpsIadA) (PDB code 2AQV) biochemical analysis suggested that the phenolic hydroxyl group in Tyr137 might function as a Lewis acid catalyst interacting with the reaction intermediate [31,32]. The bound β-isoaspartyl lysine, with the exception of the lysine side-chain region, forms tight interactions with CpsIadA. In detail, the O07 atom of the bound β-isoaspartyl lysine interacts with the ZN1 metal ion. The OG1 atom of Thr109 and the OE1 atom of Glu80 interact with the N11 atom of the β- isoaspartyl lysine, and the SG atom of Cys297 interacts with the O13 atom of β-isoaspartyl lysine. Residues Arg173 and Arg237 interact with the O01 and O04 atoms of the β-isoaspartyl lysine. As noted above, the lysine side chain of β-isoaspartyl lysine did not have any specific interactions with CpsIadA. Thus, this region has a relatively weak electron density (Figs 3B  and 4B). These results suggest that the side chain of the second amino acid in β-the isoaspartyl substrate is not critical for ligand recognition and specificity. They are also in good agreement with our activity assays that showed that CpsIadA has a broad substrate specificity with respect to the second amino acid position in the β-isoaspartyl substrate ( Table 2). In addition, a structural comparison between the β-isoaspartyl lysine complexed CpsIadA and the β-isoaspartyl histidine complexed EcoIadA (PDB code 1YBQ) revealed that bound substrates have a similar conformation with the exception of the second amino acid side chain [14]. As noted above, the biggest difference between CpsIadA and the EcoIadA structure is in the nature of the Zn ion stabilizing residue. The Glu166 residue of CpsIadA directly interacts with the Zn ions, however in EcoIadA, carbamylated lysine 162 interacts with the Zn ions. Structural superposition of CpsIadA onto EcoIadA (PDB code 1YBQ) also showed that the β12-α8 loop region (residues 165-166) in CpsIadA protruded into the active site to interact with Zn metal ions. Because of Crystal structure of isoaspartyl dipeptidase (CpsIadA) from Colwellia psychrerythraea strain 34H  this, although the Glu166 side chain length is shorter than that of carbamylated lysine, the glutamate side chain could directly interact with the Zn 2+ ion in CpsIadA (Fig 5).

Biochemical characteristics
The effect of temperature on the catalytic activity of CpsIadA was determined by incubating the enzyme at various temperatures in the presence of 10 mM β-Asp-Leu as the substrate ( Fig  6A). Under these assay conditions, CpsIadA exhibited a maximum activity at 45˚C and complete inactivation was observed at 60˚C. The thermal stability of CpsIadA was also measured. The denaturation temperature (T m ) for CpsIadA was determined to be 81˚C (Fig 6D).
Although CpsIadA was isolated from the psychrophilic bacteria, Colwellia psychrerythraea, this enzyme does not show characteristics typical of psychrophilic enzymes. Our findings suggest that CpsIadA is a thermostable enzyme, which may be attributed to its high-order oligomerization. Larger oligomeric states, such as seen here, have been observed in some hyper-thermostable proteins when compared to their mesophilic counterparts [33,34]. It has been proposed that higher order oligomerization, along with a stronger ion-pairing network and additional disulfide bridges are structure-stabilizing factors that contribute to a high thermal stability [33][34][35][36]. The isoaspartyl dipeptidase activity of CpsIadA was pH-dependent, with a maximum activity at pH 8-8.5, as shown in Fig 6B. When the pH was either decreased or increased beyond this range the enzyme activity dropped off sharply.

Substrate specificity
To investigate the substrate specificity of CpsIadA, six β-aspartyl dipeptides (β-Asp-Leu, β-Asp-Gly, β-Asp-Ala, β-Asp-Phe, β-Asp-Lys, and β-Asp-His) were evaluated as substrates, in addition to α-Asp-Leu and β-Ala-Ala (Table 2). Typical Michaelis-Menten curves were obtained for CpsIadA with all β-aspartyl dipeptides tested. Although β-Asp-Leu is the best substrate for EcoIadA, CpsIadA displayed the highest catalytic efficiencies (k cat /K m ) for β-Asp-Phe (3.0 x 10 5 M -1 s -1 ) followed by β-Asp-Leu and β-Asp-Lys. However, the k cat is greater for β-Asp-Lys than β-Asp-Phe with values of 256 and 164 s -1 , respectively. In contrast to EcoIadA, which had a two or three-orders of magnitude lower k cat /K m for β-Asp-Gly compared to other  tested β-aspartyl dipeptides, no such significant reduction in activity for β-Asp-Gly was observed for CpsIadA [14]. When α-Asp-Leu was tested as a substrate CpsIadA also exhibited dipeptidase activity with a k cat /K m of 1.4 x 10 4 M -1 s -1 , indicating that the shift of the α-amino group of aspartate moiety between C 2 and C 3 within substrates causes only slight reduction in enzyme activity. On the other hand, β-Ala-Ala was not an efficient substrate for CpsIadA, demonstrating that the αcarboxylate group of a β-aspartyl dipeptide is essential for enzyme activity. These results are consistent with those seen for EcoIadA. Overall, only slightly differences in substrate specificity were found for CpsIadA compared with EcoIadA, despite the different active site residue composition and substrate binding pocket [14].

Mutational studies of CpsIadA
We investigated the effect of active site mutations on the catalytic activity and structural integrity of CpsIadA. Four active site mutants (E80Q, Y140F, E166A, and E166K) were constructed The activity at the optimal temperature was set as 100%. (B) Effect of pH on CpsIadA activity. The pH dependence for hydrolysis of β-Asp-Leu by CpsIadA was measured at 30˚C. The activity at the optimal pH was set as 100%. (C) Hydrolysis of β-Asp-Leu by wild-type and CpsIadA mutants. The effects of single mutations on CpsIadA activity was investigated using the active site mutants E80Q, Y140F, E166A, and E166K. Reactions were performed under standard assay conditions and the activity of the wild-type enzyme using β-Asp-Leu as a substrate was taken as 100%. (D) Changes in circular dichroism spectra during thermal denaturation of wild-type and CpsIadA mutants. CD spectra were measured over a temperature range of 5-99˚C at 222 nm. https://doi.org/10.1371/journal.pone.0181705.g006 and their enzymatic activity was assessed. In addition, their denaturation temperatures (T m ) values were measured using circular dichroism (CD) spectroscopy (Fig 6C and 6D). The E80Q mutant had no catalytic activity toward β-Asp-Leu, but its CD spectra and denaturation temperature (T m ) were similar to WT, indicating that this mutation affected catalytic activity but not the overall folding and integrity of CpsIadA. In the case of the Y140F mutant, less than 10% catalytic activity was observed toward β-Asp-Leu and the CD spectra was not significantly different to the WT. Notably, unlike the WT and the other mutants Y140F had a tendency to aggregate during purification and storage, but soluble Y140F was the most thermally stable. Glu166 interacts with Zn in the active site and was substituted by mutagenesis with either Ala or Lys, an equivalent residue in E.coli. Both the E166A and E166K mutants exhibited a complete loss of activity and a considerable decrease in melting temperature. These results were probably due inhibition of Zn binding to the CpsIadA active site caused by mutation of Glu166, clearly demonstrating that Glu166 is critical for the catalytic activity and structural integrity of CpsIadA.

Phylogenetic analysis of CpsIadA
To investigate the sequence diversity and evolutionary characteristics of IadA enzymes, individual sequences were aligned and a phylogenetic tree was constructed with a total of thirtynine IadA amino acid sequences from bacteria and archaea (Fig 1 and S3 Fig). As a result, the IadA enzymes could be classified into two groups (Type I and II) based on the active site residue composition. Type I IadA enzymes have a glutamate for metal binding in the active site, whereas Type II IadA enzymes have a post-translationally carbamylated-lysine residue. Thus, CpsIadA, having Glu166, is a Type I IadA and EcoIadA, having carbamylated-Lys162, is a Type II IadA. Interestingly, bacteria in the order Enterobacteriales (class Gammaproteobacteria, phyla Proteobacteria) including E. coli and Salmonella are Type II IadAs, whereas all the other bacteria and archea are type I IadAs. From these data it can be hypothesized that Type II IadA enzymes have evolved in the direction of allowing post translational modification of the amino acid residue in the active site for metal binding and catalytic activity.
In conclusion, the crystal structure of CpsIadA presented here shows strong sequence and structural similarity to the EcoIadA but it has a different active-site residue configuration, using Glu166 instead of a carbamylated lysine residue. Interestingly, this difference is a hallmark of IadA proteins and it can be used to separate bacteria and archea into two groups (Type I and II). In EcoIadA, the carbamylation of lysine 162 residue requires a carbonate supply and time for this maturation step to occur, whereas these are not needed for CpsIadA. Thus, type I and II IadAs may have different regulatory mechanisms for controlling their activation in order to remove isodipeptides in vivo. However, it is still unclear why IadAs have evolved into two different types. Further analysis is therefore required to fully clarify the relationship between the structure and biological function of type I and type II IadAs.