{'fraction_non_alphanumeric': 0.036029621190544, 'fraction_numerical': 0.02757998670844014, 'mean_word_length': 5.203474676089517, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 2, 'https://': 0, 'lorem ipsum': 0, 'www.': 1, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
The STAT5b Pathway Defect and Autoimmunity

The signal transducer and activator of transcription (STAT) 5b is a universal transcription factor that plays key biological roles in allergic diseases, immunodeficiencies, autoimmunities, cancers, hematological diseases, growth disorders, and lung diseases. The identification of distinct pathological manifestations of STAT5b deficiency in humans has highlighted the critical role of the STAT5b pathway. Proper gene transcription at IL-2R α, FOXP3, Bcl-2, and growth hormone (GH) associated loci are thought to be associated with normal STAT5b transcriptional activity. These genes are thought to play important roles in allergy/autoimmunity, immunodeficiency, cancer/anemia, and growth, respectively. The STAT5A and STAT5B genes are collocated on 17q11. Although these two monomeric proteins exhibit peptide sequence similarities of >90%, it is known through observations of STAT5b deficient subjects that STAT5a and STAT5b are not fully redundant in humans. Patients with STAT5b deficiency have decreased numbers of regulatory CD4+CD25high T cell (Treg) despite their STAT5a levels being normal. Prior studies on STAT5b deficient subjects have revealed immunological aberrations associated with the following disease phenotype: modest lymphopenia and decreased populations of Treg, γ−δ T cells, and natural killer (NK) cells. Most subjects with STAT5b deficiency show severe eczema, and autoimmune disease (juvenile idiopathic arthritis, autoimmune thyroiditis, idiopathic thrombocytic purpura) which are thought to be associated with Treg dysfunction. We will review the likely pathophysiological mechanisms associated with STAT5b deficiency.


INTRODUCTION
The signal transducer and activator of transcription (STAT) 5b is a universal transcription factor that plays key biological roles in allergic disease, immunodeficiencies, autoimmunities, cancers, hematological disease, growth disorders, and lung disease (Buggins and Pepper, 2010;Nadeau et al., 2011). There are several differences between human and mouse in the roles of STAT5b . The identification of STAT5b deficiency in humans, and the distinct and destructive pathology associated with this deficiency has highlighted the critical role the STAT5b pathway. Research on the immunologic function of STAT5b has demonstrated its importance for the in vivo accumulation of regulatory CD4 + CD25 high T cells (Treg) with immunoregulatory function (Cohen et al., 2006;Nadeau et al., 2011). The specific role that STAT5b plays in the pathogenesis of the aforementioned diseases has led to suggestions that the transcription factor might have potential as a novel diagnostic and/or therapeutic target in some disease settings.
In this review, we summarize recent advances in our understanding of the STAT5b pathway in human mainly as well as the autoimmune manifestations induced by the defects within it.
Although STAT5a and STAT5b show peptide sequence similarities of >90%, they differ by six amino acid in the DNA binding domain and 20 amino acids in their carboxy termini (Boucheron et al., 1998;Grimley et al., 1999;Soldaini et al., 2000;Wei et al., 2008). Additional reports of a common disease phenotype specifically associated with STAT5b deficiency in humans (but no such phenotype associated with STAT5a deficiency) indicates that, at least in humans, the roles of STAT5a and STAT5b are not fully redundant .
Structural dissimilarities between the STAT5a and the STAT5b on transactivation domains or subtle differences in the DNA binding affinities of STAT5 dimer pairs could influence gene regulation, but cell-dependent asymmetries in the availability of phosphorylated STAT5a or STAT5b could also another factor. Signal attenuation by phosphatase action or classic feedback inhibition, or truncated forms of STAT5b lacking in transactivation capacity, may compete upstream for activation and diminish access of full length molecules to DNA binding sites (Grimley et al., 1999). Thus, both STAT5 proteins could bind to the same targets, and any differences between STAT5a and STAT5b may arise from differential expression or difference in kinetics of DNA binding (Grimley et al., 1999).

UPSTREAM OF STAT5b: CYTOKINES AND THEIR RECEPTORS
Signal transducer and activator of transcription 5b is a common downstream effector of the IL-2, -4, -7, -9, -13, -15, -21, growth hormone (GH; Liu et al., 1997), erythropoietin, thrombopoietin, and granulocyte colony-stimulating factor signaling molecules FIGURE 1 | The STAT5B gene is collocated on 17q11.2 approximately 12 kb apart from the STAT5A gene. The STAT5B gene is on the negative strand, and the STAT5a gene is on the positive strand. The genomic size of STAT5b is 58,700-77,229. The genomic size of STAT5a is approximately 24,000. . Each cytokine has associated receptors, and each receptor has associated Janus kinases (JAK). For example, the IL-2 receptor is composed of an α chain (CD25), β chain (CD122), and γ chain (CD132; Lin and Leonard, 2000). The β chain is associated with JAK1 and JAK3 (Zhu et al., 1998) and the γ chain is associated with JAK3 (Figure 2; Russell et al., 1995). The growth hormone receptor (GHR) is associated with JAK2 .
The CD25 plays an important role as an integral component of the high affinity IL-2 receptor. Its ligand, IL-2, is a cytokine known for the role it plays in lymphocytic function, especially with relation to T cell biology. There are two functional receptors for IL-2: one is a heterodimeric complex formed by the β and γ chains, while the other is a trimeric membrane-spanning complex composed of the α, β, and γ subunits. The latter receptor has a higher affinity for IL-2 than the former (Lin and Leonard, 2000). Additionally, defects in STAT5b expression and function have been shown to result in reduced expression of IL-2Rα, thereby potentially limiting cellular response to IL-2 signaling (Cohen et al., 2006).
The engagement between cytokines and their cell surface receptors results in subsequent activation of receptor-associated JAK tyrosine kinase activity. Activated JAKs phosphorylate specific tyrosine resides in the cytoplasmic domain of their associated receptor, and these newly phosphorylated residues serve as docking sites for STAT proteins (Figure 2; Grimley et al., 1999).

PHOSPHORYLATION OF STAT5b BY JAKs (MAINLY JAK1 AND 3)
Intracellular signal transduction pathways are essential for transforming extracellular cytokine signaling into appropriate cellular responses. The phosphorylation of STAT molecules is a key component in the JAK/STAT signal transduction pathway (Xu and Qu, 2008).
Cytokine engagement of membrane-associated receptors brings receptor subunits into proximal relationships necessary for JAK autophosphorylation (Figure 2). Cytoplasmic STAT monomers are subsequently able to bind the phosphotyrosine residues on engaged cytokine receptors through the highly conserved SH2 domain located on all proteins of the STAT family (Figure 3).
As a result of this docking, JAK and STAT molecules are brought into close enough proximity to allow for JAK phosphorylation, and therefore activation, of STAT molecules. In the case of STAT5, phosphorylated STAT5a and/or STAT5b then homo-or heterodimerize (sometime tetramaerization; John et al., 1999;Soldaini et al., 2000;Mandal et al., 2011) by each SH2 domain, leave the receptor, and translocate to the nucleus where they act as a transcriptional activator for each target gene (Levy and Darnell, 2002).

DOWNSTREAM OF THE STAT5b PATHWAY
Signal transducer and activator of transcription 5b dimers translocate into the nucleus and bind to specific regions thought to be associated with transcription of FOXP3, CD25, Bcl-2, IGF-1 . Reports indicate that STAT5b may preferentially interact with different DNA binding sites depending on the cell type considered.

Fork-head box P3 (FOXP3): a key transcription factor essential for Treg cell development and function
The transcription factor FOXP3 is critical for the thymic development of Tregs (Sakaguchi et al., 2008). In mice, CD4 + CD25 + peripheral T cells and CD4 + CD25 + CD8 − thymocytes express Foxp3 and are considered to be immunoregulatory, whereas other thymocytes/T cells, either in a resting or activated state, do not (Fontenot et al., 2003;Hori et al., 2003;Khattri et al., 2003;Sakaguchi et al., 2008).
Studies investigating the effects of FOXP3 suppression report complications associated with Treg dysfunction to be a main pathological consequence. Mutations of the FOXP3 gene were found to be the cause of an IPEX (immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome), which is characterized by autoimmune disease in multiple endocrine organs (as in type I diabetes and thyroiditis), inflammatory bowel disease, and severe allergy (Chatila et al., 2000;Bennett et al., 2001;Wildin et al., 2001). Deletion or dysfunction of FOXP3 causes impaired function and/or homeostasis of Tregs, and has been implicated in the development of several common autoimmune and inflammatory diseases (Campbell and Koch, 2011).

The essential role of CD25 in Treg development and function
High expression of CD25 is considered to be a marker of Tregs (Sakaguchi et al., 1995) and studies have elaborated on this concept, demonstrating that the IL-2Rα serves not only as a marker for natural Treg, but also, as a protein essential for its development and function (Sakaguchi et al., 2008). The importance of CD25 in the development of a normal immune response is emphasized by the finding that a truncation mutant of CD25 results in an immunodeficiency in humans characterized by an increased susceptibility to viral, bacterial, and fungal infection (Sharfe et al., 1997). In addition, gene targeting analysis also reveals that CD25 deficient mice exhibit autoimmunity (Willerford et al., 1995).
While CD25 contributes to IL-2 binding affinity and not to the recruitment of signaling molecules (Lin and Leonard, 2000) its role as a component of the high affinity IL-2 receptor makes it indispensable for the activation of cell signaling pathways associated with IL-2 signal transduction (Sakaguchi et al., 2008). The engagement between a cytokine and its cell surface receptor results in subsequent activation of receptor-associated JAK. Activated JAK phosphorylates specific tyrosine resides in the cytoplasmic domain of the receptor which in turn serves as the docking sites for STAT5a and/or STAT5b. STAT5a and/or STAT5b are recruited to the phosphorylated receptor and subsequently phosphorylated by JAKs. The phosphorylated STAT5a and/or STAT5b dimerize, leave the receptor, and translocate to the nucleus.

Bcl-2 is an apoptotic inhibitor protein
Bcl-2 is an apoptosis inhibitor protein. Most cell death in vertebrates occurs via the mitochondrial pathway of apoptosis, in which Bcl-2 and other anti-apoptotic proteins (Bcl-xL, Bcl-w, Mcl-1, and Bfl-1/A1) are key effectors (Llambi and Green, 2011). Aberrant regulation of Bcl-2 has been reported to cause or correlate with autoimmunity or cancer, particularly leukemia (Buggins and Pepper, 2010;Tischner et al., 2010). Deletion of self-reactive immune cells occurs through this apoptotic pathway and is necessary for the maintenance of immune tolerance (Tischner et al., 2010). Overexpression of Bcl-2 has been noted in patients with systemic lupus erythematosus (Tischner et al., 2010). In malignant diseases, decreased rate of apoptotic cell death is also found to be responsible in the proliferative process (Ulukaya et al., 2011).

Insulin-like growth factor-I and Insulin-like growth factor binding protein-3 play an important role in fat metabolism and skeletal development
Insulin-like growth factor-I promotes skeletal development and fat metabolism, and insulin-like growth factor binding protein-3 (IGFBP-3) acts as a negative regulator for IGF-I signaling (Kawai and Rosen, 2010).
The activation of IGF-I is initiated by the interaction of circulating GH with the GHR. The cytoplasmic domain of GHR associates preferentially with JAK2. Activation of JAK2 by GHR engagement leads to the activation of STAT5b .
In humans, serum IGF-I concentrations have a positive correlation with skeletal mass (Langlois et al., 1998). A report on the disease characteristics of STAT5b deficiency in humans highlights low serum IGF-1 as one defining clinical feature of the disease (Hwa et al., 2004;Nadeau et al., 2011). STAT5b deficient patients also exhibit stunted growth and poor response to GH therapy .
IGF-I was also reported as a critical factor for adipogenesis (Kawai and Rosen, 2010). The lack of this factor results in a defect in adipose tissue formation by mitogen-activated protein kinase deactivation in conjunction with GH (Boney et al., 2000;Hwa et al., 2011).

HUMAN STAT5b PATHWAY DEFECT AND AUTOIMMUNITY
Human STAT5b deficiency is a recently identified, rare autosomal recessive disease that involves both severe GH-resistant growth failure and severe primary immunodeficiency. It was first discovered in patients with dwarfism associated with normal levels of serum GH, but very low levels of IGF-I (Kofoed et al., 2003;Bernasconi et al., 2006;Chia et al., 2006). Affected individuals also exhibited recurrent infections, chronic diarrhea, eczema, and/or lymphocytic interstitial pneumonitis (Kofoed et al., 2003;Bernasconi et al., 2006;Chia et al., 2006). Immunophenotyping of these patients have revealed modest lymphopenia and decreased populations of Treg, γ-δ T cells, and natural killer (NK) cells (Bernasconi et al., 2006;Cohen et al., 2006). There are currently 10 published cases of STAT5b deficiency ( Table 1; Nadeau et al., 2011). Ongoing research efforts aim to identify the molecular mechanisms of STAT5b in postnatal growth and immunity.

Previous cases
The first case of a STAT5b mutation was reported in 2003, in a 16-year-old female with severe growth retardation (−7.5 SD) and pulmonary complications (Kofoed et al., 2003). The reported missense mutation (p.A630P) disrupted the core of anti-parallel β-sheets that enable phosphate-binding, causing aberrant folding (Chen et al., 1998) aggregation of mutant STAT5b protein, and loss of thermodynamic stability (Chia et al., 2006;Fang et al., 2006). The patient presented with early onset lymphocytic interstitial pneumonitis, chronic lung disease, hemorrhagic varicella, atopy, and autoimmune disease (Kofoed et al., 2003). At age 7, she developed lymphocytic interstitial pneumonia and after receiving potent immunosuppressive therapy, had two major infectious complications -severe varicella-zoster virus infection and Pneumocystis jiroveci pneumonia. Another biopsy at age 10 also indicated lymphoid interstitial pneumonia, and P. carinii was isolated from the tissue. Later studies revealed decreased numbers of Treg and reduced Treg suppressive function (Cohen et al., 2006).
In 2005, a second case of a STAT5b deficiency was identified in a 16-year-old Turkish female with severe growth failure, GHI, atopic dermatitis, pruritic skin lesions, primary idiopathic pulmonary fibrosis with diffuse lung involvement, and autoimmune disease, as well as bleeding diathesis caused by defective thrombocyte aggregation, preventing a potential lung biopsy (Hwa et al., 2005). Sequencing of the STAT5b gene revealed a novel homozygous frameshift mutation (c.1191insG) that led to protein termination (p.N398EfsX16) and consequent lack of immunodetectable STAT5b protein (Hwa et al., 2005).
Another case was identified in 2006 in a 16-year-old female with severe postnatal growth failure, GHI, and immunodeficiency   www.frontiersin.org (Bernasconi et al., 2006). Pulmonary-function tests showed mixed, restrictive, and obstructive moderate ventilative insufficiency, but no lung biopsy was performed. Notably, this case was the first to identify a role for STAT5b not only in the human GH signaling cascade, but also in the cytokine-mediated immune response. The STAT5b deficient patient had moderate T cell lymphopenia, normal CD4/CD8 ratios, and very low numbers of NK cells and γ-δ T cells, and the T cells presented a chronically hyperactivated phenotype (Bernasconi et al., 2006). Since 2012, five other mutations have been published on a total of seven additional subjects ( Table 1). Lung pathology has been common among these patients (8 of 10), but of these remaining seven subjects, only a few have received lung biopsies. A STAT5b deficient male with the mutation 424_427del received a biopsy at 6 years of age that indicated severe lymphocytic interstitial pneumonitis. Considered together, these studies have firmly established a correlation between STAT5b deficiency and immune dysfunction, in addition to GHI and severe growth problems.

Clinical manifestations and diagnosis
Signal transducer and activator of transcription 5b deficiency should be considered in the differential diagnosis of a patient who has normal gestational growth and birth size but acquires significantly short stature and recurrent infections. This pattern of growth is typical of patients with GHI. Height may range from −3.0 to −9.9 SD in girls and boys, respectively ( Table 1).
Regarding hormone evaluations, all described patients have had normal levels of GH at baseline, but after stimulation, GH concentrations were often elevated ( Table 1). In contrast, serum IGF-I, IGFBP-3, and acid labile subunit concentrations were low, and even upon administration of GH, remained low. Elevated prolactin levels were also observed in patients with recorded concentrations.
Most patients have displayed evidence of immune dysfunction, including atopic disease, chronic lung disease, viral infections, and/or autoimmune diatheses. Often present in childhood, severe pulmonary disease is of particular concern, as it has affected 8 of the 10 known STAT5b deficient patients and two patients have died of respiratory failure. For all cases of lung pathology except for that of Patient #5, an axial chest CT scan has shown increased interstitial patterns and ground-glass appearance. These pulmonary lesions are T cell predominant, despite peripheral lymphopenia. In most cases, severe eczema, thrombocytopenic purpura, and/or autoimmune disease, such as juvenile idiopathic arthritis, were present in addition to severe lung disease. However, it should be noted that 1 of the 10 subjects to date has less severe immune dysfunction. Congenital ichthyosis was diagnosed at birth, and the patient had hemorrhagic varicella at 16 years of age but had no history of pulmonary of immunological problems (Vidarsdottir et al., 2006).
Previous immunological studies have established the importance of STAT5b proteins in the development, homeostasis, and proliferation of different lymphocyte populations. Immune repertoires of STAT5b deficient patients have shown moderate lymphopenia, with very low numbers of NK and T cells, as well as Treg dysfunction. Furthermore, B cell populations and immunoglobulin G levels in at least patient are normal to elevated, as consistent with autoimmune disease symptoms (Cohen et al., 2006).

Disease management
In order to improve clinical outcomes for patients with STAT5b deficiency, optimizing early diagnosis in these patients is critical. To date, overall management of STAT5b deficiency is still unclear. GH therapy is ineffective due to the patients' GHI. It is presumed that IGF-I therapy may be an effective treatment, unless the presence of chronic infection limits the growth response. However, to date, no clinical trials of IGF-I therapy have been performed in these patients.
Patients should be closely monitored for signs and symptoms of immunodeficiency. Infections such as severe varicella or recurrent pneumonias should be aggressively treated with appropriate antimicrobial therapies. Patients with autoimmune conditions, atopic diseases, or pulmonary fibrosis may also require antiproliferatives or immunosuppressants, such as steroids, to address overactive effector T cell responses. Because severe chronic lung disease in this patient population often leads to high morbidity and mortality, patients should be carefully monitored with pulmonary-function tests and physical examinations, which may improve treatment options to decrease the lung disease severity.
Although current management of STAT5b deficiency is primarily dictated by specific end-organ pathology, current research is addressing the possibility of enhancing STAT5b and/or STAT5a pathways (Zeiser et al., 2008;Strauss et al., 2009). Future therapy may be expected to prevent and reflect rationally based drug design to enhance certain drug targets in the STAT5b and/or STAT5a pathways.

CONCLUSION
In this review, we focused on the STAT5b pathway and the mechanisms by which defects in protein structure and or expression might result in autoimmunity. A better understanding of STAT5b and its distinct biological functions is necessary for the development of new diagnostic and therapeutic approaches for treating patients suffering from its deficiency.