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Artificial Inoculation of Wheat for Selecting Resistance to Stagonospora Nodorum Blotch.

In the eastern United States, natural epidemics of Stagonospora nodorum blotch (SNB) are not consistently severe enough to facilitate substantial progress in breeding moderately resistant cultivars of soft red winter wheat. We compared three artificial inoculation methods to natural inoculum in a field experiment involving seven wheat (Triticum aestivum) cultivars with varying levels of SNB resistance. Artificial inoculation methods were: Phaeosphaeria nodorum conidia applied by atomization to three- to four-leaf wheat in early winter, P. nodorum conidia applied by atomization at boot stage in late spring, and P. nodorum-infected wheat straw applied in early winter. The experiment was conducted at Kinston and Plymouth, NC, in 2003-2004, 2004-2005, and 2005-2006, and all treatments had three replicates. Percent diseased canopy was assessed and comparisons were made using disease severity at a single date (early to soft dough stage) and area under the disease progress curve (AUDPC). The relative resistance level of cultivars was consistent across sites, years, and inoculum methods, although the rankings of moderately susceptible and susceptible cultivars were sometimes switched. On average, late spores and straw caused significantly more disease than early spores or natural inoculum (P ≤ 0.05). Biplot analysis indicated that all artificial methods had a higher mean capacity to discriminate among cultivars than did natural inoculum (P ≤ 0.05). On average, artificial inoculation increased the capacity of environments to separate wheat cultivars by SNB resistance.