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First rotavirus, now astrovirus: the evolving benefits of RT-PCR.

We compared RT-PCR and a newly developed amplified EIA for the detection of human astrovirus (HAstV) in 48 stool samples taken from children of less than five years of age with gastroenteritis. The RT-PCR classified more samples as positive than the EIA (18 versus 8). Without sensitive diagnosis, infection control policies will be disadvantaged and the true economic burden of HAstV underestimated.