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A fragment of the neurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo

The basic helix‐loop‐helix transcription factor neurogenin1 is required for proper nervous system development in vertebrates. It is expressed in neuronal precursors during embryonic development and is thought to play a role in specifying neuronal fate. To investigate the regulation of neurogenin1 expression, the transcriptional start site of the gene was identified and a 2.7‐kb fragment ending in the first exon was shown to possess basal promoter activity. This 2.7‐kb fragment was able to promote expression of reporter genes in P19 cells under conditions in which expression of endogenous neurogenin1 was induced. Importantly, the 2.7‐kb fragment was able to drive expression of a lacZ reporter gene in transgenic mice in most tissues in which neurogenin1 is normally expressed, including those peripheral ganglia that fail to develop in neurogenin1 “knockout” mice. These findings identify a regulatory region containing elements responsible for appropriate expression of a gene with a crucial role in generating the vertebrate nervous system. Dev Dyn;218:189–194. © 2000 Wiley‐Liss, Inc.