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A modified, sensitive liquid chromatographic method for measurement of propranolol with fluorescence detection.

A simple, sensitive, and rapid fluorometric-high-performance liquid chromatographic method for quantitating human and animal plasma and blood levels of propranolol has been developed. The procedure, which requires microliter volumes of triethylamine in the mobile phase mixture and microliter volumes of plasma or blood (50-100 microL), may be applied in routine clinical analysis as well as in pharmacokinetic studies using small laboratory animals. A single, selective extraction step precedes the analysis. The limiting concentration that can be detected is 1 ng/mL.