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GROWTH AND CHARACTERISATION OF A HUMAN COLONIC MUCIN SECRETING CELL LINE HT29‐18N2

A number of cell lines have been established from human colonic adenocarcinoma turnouts (Fogh et a1 1977) and are used as in vitro models for gastrointestinal absorption and transport (Wilson et a1 1990). It has been claimed that the HT29-18N2 clone, which represents a highly differentiated species produced by treatment of HT29 cells displaying absorptive properties with sodium butyrate (Huet et a1 1987; Phillips et a1 1988), secretes mucus. If this is so, then the validity and potential of the model are increased since the effects of the mucus barrier on absorption can be investigated in vitro.