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Factors associated with antiretroviral treatment interruption in human immunodeficiency virus ( HIV )-1-infected children attending the Jos University Teaching Hospital , Jos , Nigeria

Background: Interrupting anti-retroviral therapy (ART) for any number of reasons is an indication of a compromised adherence to ART. Several factors, including the pill burden from other drugs used in treating co-infections in children with human immunodeficiency virus (HIV), may influence ART adherence. The aim of this study was to identify the factors associated with ART interruption in HIV-1-infected children. Materials and Methods: A retrospective cohort study analysing data on 580 children consecutively enrolled on ART between February 2006 and December 2010 at the paediatric HIV clinic of Jos University Teaching Hospital (JUTH), Jos. Subjects were children aged 2 months — 15 years diagnosed with HIV-1 infection and on first-line ART. Cotrimoxazole prophylaxis was usually commenced at diagnosis while awaiting ART commencement. Children diagnosed with tuberculosis (TB) were also placed on multiple individual anti-TB drugs. Statistical analysis used: A comparison of the data on children with and without ART interruption was made. Variables associated with ART interruption in a univariate analysis were fit in a multivariate logistic model to determine the factors that were associated with ART interruption. Results: Children on anti-TB drugs were twice more likely to interrupt ART compared to those who were not, (adjusted odds ratio, AOR = 1.84 (1.03-3.28); P = 0.04). But children on cotrimoxazole prophylaxis had a 57% reduction in the odds of interrupting ART compared to those who were not, (AOR = 0.43 (0.200.93); P = 0.03). Conclusion: Children on ART and also taking multiple individual anti-TB drugs should be monitored closely for ART adherence. Cotrimoxazole prophylaxis should be encouraged in children diagnosed with HIV while awaiting ART commencement as this may prime them for a better ART adherence.


INTRODUCTION
Human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS) continues to constitute a major health problem in sub-Saharan Africa (SSA) where it is estimated that 2.9 million children were Interrupting ART is an indication of a compromised adherence to ARVs. Socio-economic, cultural, behavioural and clinical 12,[15][16][17][18][19][20] factors have been reported to be associated with poor ART adherence in children. Few studies 12 have looked at the influence of other medications taken along with ARVs, on adherence to ART. Also, pill burden may affect adherence to ART 16 ; with one study showing that, the rate of adherence improves when the pill burden is low. 21 Drugs, such as cotrimoxazole, used for prophylaxis against opportunistic infections (OIs) and anti-tuberculosis (anti-TB) drugs, are commonly used in children with HIV/ AIDS and these may affect ART adherence. In this study, we determined the factors associated with ART interruption in HIV-1 infected children attending the HIV clinic at the Jos University Teaching Hospital, Jos.

MATERIALS AND METHODS
This was a retrospective cohort study which analysed data on 580 children who were consecutively enrolled on ART between February 2006 and December 2010. Study subjects were children aged 2 months -15 years diagnosed with HIV-1 infection at presentation to the AIDS Prevention Initiative in Nigeria (APIN)-supported paediatric HIV clinic at the Jos university teaching hospital (JUTH), Jos, Nigeria and who were subsequently commenced on first-line ART. A written informed consent was obtained from the parents/guardians of the children for use of the data for research as approved by the Ethics committee of the Jos University Teaching Hospital.
The data obtained included the following: Demographic (age and sex); clinical (the use of cotrimoxazole for opportunistic infections prophylaxis, treatment with anti-TB drugs, treatment with ARVs, World Health Organisation (WHO) HIV clinical stage, oral thrush and weight-for-age z score (WAZ)) and laboratory (haemoglobin (Hb) level, viral load, absolute cluster of differentiation 4 (CD4) cell count). In this study, ART interruption was defined as failure to take medications (missed more than 2 doses or missed more than 1 day of ART) during any 4 weeks follow-up period. Each child had a total of seven followup visits over a period of 8 months since starting ARVs. And the common reasons for interrupting treatment include: Forgot to take medication, caregiver travelled and medicine not available.
The diagnosis of HIV and the criteria for commencement of ART in children was based on the Nigeria's National Guidelines for Paediatric HIV and AIDS Treatment and Care. 22 Data on treatment failure was also obtained. Treatment failure was either virological or immunological or both; with or without clinical failure and defined using the Nigeria national guidelines for paediatric HIV and AIDS treatment and care. 22 The diagnosis of TB was based on WHO guidelines for the management of TB in children. 23 Medications used by study subjects: At diagnosis of HIV infection, prior to starting ART, all children were usually commenced on cotrimoxazole for prophylaxis against OIs. Children diagnosed with HIV were placed on first-line ART which could be any one of the following combinations: Zidovudine (AZT) + Lamivudine (3TC) + Nevirapine (NVP) or AZT + 3TC + Efervirenz (EFV); and the dosing regimen were twice a day for AZT, 3TC & NVP or once a day for EFV. 22

Statistical analyses
ART interruption was the outcome variable (a binary variable). Children who had ART interruption were compared with those who did not. All other variables were regarded as independent variables. The weightfor-age z score (WAZ) was determined from the weight of the children, adjusted for age and sex, using the WHO AnthroPlus software 24 by importing the variables -weight, age and sex in the form of a text file into the software. The WAZ was then categorised into a binary variable using the WHO cut-off of Z < -3 with WAZ < -3 defined as severe malnutrition. 25 WHO clinical stage was stratified into stage 3 or 4 versus stages 1 or 2 and HIV RNA viral load categorised into <10.5 log 10 versus ≥10.5 log 10 copies/mL using the median cut-off value.
The association between each independent variable and the outcome was examined using the Chi squared or Fisher's exact test for categorical variables while the Wilcoxon-Mann-Whitney test was used for comparison of two medians. The association between the independent variables and outcome was examined using univariate logistic regression. Variables that were associated with treatment failure in the univariate analyses at P <0.02 were considered for inclusion in the multivariate model. Age and sex were included a priori in the multivariate model since these could influence disease processes. A backward stepwise modelling strategy was used in building the final multivariate model. The area under the receiver operating characteristic (ROC) curve was determined to assess the performance of the model. Results of the logistic regression analyses were expressed as odds ratios (ORs) with their 95% confidence intervals (CIs). All analyses were performed using Stata software version 10.0 (Stata Corporation, College Station, Texas, USA) and all tests were two-sided with a P value of <0.05 considered statistically significant.
In the unadjusted logistic regression analysis, the odds of ART interruption was about twice more in children on anti-TB drugs compared to those who were not (OR = 1.95) and this was significant (P = 0.005). The odds of ART interruption was reduced by about half in those who were on cotrimoxazole prophylaxis compared to those who were not (OR = 0.56) but not significant (P = 0.06). Though the odds of ART interruption was more: In those who failed first-line ART compared to those who did not (OR = 1.52), in those with HIV RNA viral load ≥10.5 log 10 copies/mL compared to those with less (OR = 1.53) and in those with Hb <8.0 g/dL compared to those with higher levels (OR = 1.51), these findings were not significant (p = 0.09, p = 0.10 & 0.35 respectively). Also, despite a reduced odds of ART interruption in males compared to females (OR = 0.66) this was not significant (P = 0.05) [ Table 2].
In the multivariate analysis, only the use of anti-TB drugs and cotrimoxazole for prophylaxis were significantly associated with ART interruption. Children on anti-TB drugs were twice more likely to interrupt ART compared to those who were not, Adjusted Odds Ratio (AOR) = 1.84; 95% CI (1.03-3.28); P = 0.04). But children on cotrimoxazole prophylaxis had a 57% reduction in the

DISCUSSION
Being on anti-TB drugs and on cotrimoxazole where the factors independently associated with ART interruption.
In our study, we found a strong association between taking multiple single anti-TB drugs along with ARVs and ART interruption which is an indicator of compromised ART adherence. Taking multiple individual anti-TB drugs as in our study as against taking FDC anti-TB drugs increased the pill burden. To support our finding, one meta-analysis study involving patients with various chronic diseases including tuberculosis and HIV infection, showed that adherence was better in those who took fewer pills in the form of FDCs. 26 Another study by O'Connor et al. in patients on ART also showed an association between a greater pill burden and suboptimal adherence. 21 Our observation of a reduction in ART interruption in children on cotrimoxazole is supported by the findings of Biadgilign et al. 17 and Ebonyi et al. 27 that children on ART who also took cotrimoxazole, were more likely to adhere to ART. This may be so because being on cotrimoxazole prophylaxis even prior to commencing ART had already familiarised the children and caregivers to good adherence.
One of the limitations of our study was that we did not assess the influence of the adverse effects of ARVs or anti-TB drugs on ART adherence which could be confounding factors in the association between anti-TB drugs and ART treatment interruption. It is well documented that the side effects of these drugs could interfere with ART adherence. 28,29 Another limitation was our inability to measure the potential confounding effects of socioeconomic, cultural or behavioural factors 12,15-20 on the association between our identified independent variables and ART interruption.

CONCLUSION
Being on anti-TB drugs and cotrimoxazole prophylaxis were the factors identified to be associated with ART interruption. Children on ART and also taking anti-TB drugs should be monitored closely for ART adherence. Cotrimoxazole prophylaxis should be encouraged in children diagnosed with HIV while awaiting ART commencement as this would prime them for a better ART adherence.