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Linkage of K99 Production and STa Activity in a Plasmid of an Escherichia coli Porcine Isolate

An Escherichia coli strain ZP118 of porcine origin was shown to harbor a 68‐megadalton (Md) plasmid coding for a colonization factor K99 and heat‐stable enterotoxin (STa), and a self‐transmissible 51‐Md plasmid coding for drug resistance. One of the transconjugants obtained by mating between ZP118 and E. coli C was found to harbor a 90‐Md plasmid coding for K99, STa and drug resistance. Restriction endonuclease analysis suggested that the 90‐Md plasmid could be a recombinant between the 68‐Md plasmid and the 51‐Md plasmid.