{'fraction_non_alphanumeric': 0.029674537332482452, 'fraction_numerical': 0.022016592214422464, 'mean_word_length': 4.9647954329210275, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Transcriptomic profiling comparison of YAP over-expression and conditional knockout mouse tooth germs

To identify the downstream target genes of YAP, we used RNA-Seq technology to compare the transcriptomic profilings of Yap conditional knockout (Yap CKO) and YAP over-expression mouse tooth germs. Our results showed that some Hox, Wnt and Laminin family genes had concurrent changes with YAP transcripts, indicating that the expression of these genes may be regulated by YAP. Here, we provide the detailed experimental procedure for the transcriptomic profiling results (NCBI GEO accession number GSE65524). The associated study on the regulation of Hoxa1 and Hoxc13 genes by YAP was published in Molecular Cellular Biology in 2015 [Liu et al., 2015].


Animal usage
The human keratin 14 promoter (K14) was used to either conditionally knockout Yap or drive YAP transgenic over-expression in mouse embryonic ectoderm-derived epithelial tissues. Yap conditional knockout (CKO) embryos were generated through crossing Yap fl/fl mice with K14-Cre mice [1,2] in C57BL/6 background. YAP over-expression (OE) embryos were generated through breeding Col-TetO-YAP S127A mice with K14-rtTA mice [1][2][3][4][5] in FVB/NJ background, in which the constitutively active form of human YAP1 protein with the Ser127Ala mutation was over-expressed upon Doxycycline (Dox) administration. 2 mg/ml of Dox in drinking water was given starting from embryonic days 9.5 (E9.5) to E14.5. All mouse studies were performed in compliance with the protocols approved by the Harvard University Institutional Animal Care and Use Committee.

E14.5 embryonic tooth germ dissection, collection and RNA preparation
Three Yap CKO embryos at E14.5, along with the three corresponding three litter controls, were used for tooth germ collection of the Yap CKO group. Similarly, three YAP over-expression and three corresponding litter control embryos at E14.5 were used for the YAP OE group. The tooth germs in lower jaws were collected under microscopic dissection. The tooth germs were sequentially broken down by 18, 21 and 25 gauge needles in TRIzol (Life Technologies). Then total RNA was extracted and purified using RNAeasy Mini Kit (Qiagen) with on-column DNase (Qiagen) digestion, and evaluated by the Agilent 2100 bioanalyzer (Agilent Technologies, CA). The RNA integrity numbers of all 12 samples were 10 ( Fig. 1), indicating high quality of the RNA samples. To minimize the individual difference from embryos, each RNA-Seq sample was pooled from the three biologically different E14.5 tooth germs with the same amount of total RNA.

Contents lists available at ScienceDirect
Genomics Data

Data analysis
Mus musculus genome (mm9) and DNAnexus software were used to extract sequencing reads of genes from the RNA-Seq raw data. RNA-Seq raw sequencing reads and aligned reads are available through the Gene Expression Omnibus at the accession number of GSE65524. After RNA-Seq reads were extracted and aligned with the mm9 genome, the relative read ratio of each gene was calculated and further analysis was conducted. We compared the transcriptomic profilings between the Yap CKO and wild type tooth germs, as well as those between the YAP OE and wild type mouse tooth germs. In comparison with the corresponding controls at the 1.5-fold change cutoff, there were 968 down-regulated genes and 979 up-regulated genes in Yap CKO tooth germs, while there were down-regulated 1289 genes and upregulated 774 genes in YAP over-expression tooth germs (Fig. 2). Further analysis revealed many differentially expressed genes between Yap CKO and YAP OE tooth germs. Interestingly, we found that some genes in the Hox, Wnt and Laminin families were differentially expressed in the two kinds of the tooth germs. The transcription levels of several Hox gene family members were decreased in Yap CKO tooth germs while their expression levels were increased in YAP OE tooth germs (Fig. 3), indicating that these genes could be potential downstream targets of YAP in vivo. The regulation of the Hoxa1 and Hoxc13 gene expression by YAP was further validated and functionally analyzed in both mouse and human epithelial cells [1].
Similar to the expression changes of the Hox genes, several Wnt and Laminin family members also showed concurrent changes with YAP transcripts (Fig. 3), although the members of these two gene families do not have a linear genomic arrangement pattern. The members of the Wnt family showing concurrent changes include Wnt10b, Wnt16, Wnt3, Wnt3a, Wnt4, Wnt6, Wnt7a and Wnt9b. The transcript levels of the latter six Wnt genes were further validated by qPCR in both tooth germs and skin samples. The qPCR results were consistent with those from RNA-Seq results. The members of the Laminin gene family with concurrent changes consist of Lama3, Lama5, Lamb3 and Lamc2.

Discussion
In this study, we used RNA-Seq analysis to compare the transcriptomic profilings of Yap CKO and YAP over-expression mouse tooth germs. We found that some genes in the Hox, Wnt and Laminin families exhibiting concurrent changes with YAP transcripts and may be potentially targets of YAP. The regulation of Hoxa1 and Hoxc13 genes by YAP was further validated and functionally analyzed in different epithelial cells of mouse tooth germs, skin samples and human keratinocytes [1].
The transcriptomic profiling comparison revealed that Hoxa1, Hoxa2, Hoxa3, Hoxa5, Hoxb9, Hoxc13, Hoxc4, Hoxc8 and Hoxd1 have differential expression in Yap CKO and YAP OE tooth germs. However, our qPCR results showed that only Hoxa1 and Hoxc13 had significant concurrent changes in their transcripts. This discrepancy could be due to low abundance of the Hox genes in mouse tooth tissues, which may cause high relative ratios.
Previous studies demonstrated that Wnt/β-catenin signaling regulates YAP expression in vitro [6,7]. However, how YAP affects the Wnt family members remains unknown. There are also some other gene families showing concurrent transcript changes with YAP transcripts, such as the Laminin, Rho GTPas activating protein and Ras-related  protein gene family members. Further investigation is required to understand the regulation of their expression by YAP and the relationships among these gene families.