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Analysis of the I_(D2) Component of Endogenous ATP-Induced Inward Currents in Follicular Oocytes of Xenopus Laevis

The experiments were performed on follicular Xenopus laevis oocytes using two-electrode voltage-clamp technique and intracellular dialysis method. ATP applied externally could induce endogenous membrane currents, which consisted of three components, i.e. I D1 , early fast deplarizing inward current; I D2 , late slow lasting depolarizing inward current and I H, slow hyperpolarizing outward current. The present study aimed to analysis the characteristics of I D2 components of ATP-induced currents (I ATP ) and their underlying mechanisms. The results observed are as follows: (1) The amplitude of I D2 was attenuated by around 50% 10 min after intracellular dialysis of 500 μmol/L GDP-β-S and almost completely abolished 30 min after the dialysis; (2) With the decrease in Cl - content in Ringer's Solution from 100 to 5 mol/L via substituting SO 2- 4 for Cl - in Ringer's solution, the amplitude of I D2 component of ATP (100 μmol/L)-induced current increased markedly, and the reversal potential shifted from (-20±2.1) mV to (+1.35±0.75) mV. (3) After displacing NaCl in Ringer's solution with equimolar choline chloride, the amplitudes of I D2 at the 4 different concentrations of ATP from 1 to 1000 μmol/L were higher than the controls markedly. It comes to the conclusion that in Xenopus oocytes I D2 , a Ca 2+ dependent Cl - current, is mediated by P 2Y purinoceptor via G protein coupling and corresponding intracellular signal transduction process.