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Banding and preparational techniques in normal and neoplastic tissue. I. Human lymphocyte and bone marrow cultures.

The usefulness of in vitro banding techniques depends on the yield of suitable metaphases and the quality of the induced bands. This paper describes optimal synchronization and R-banding techniques for human lymphocyte and bone marrow cultures. Chromosome analysis of leukemic bone marrow is summarized. The lymphocyte and bone marrow cell systems are compared with regard to optimal culture time and banding potential. It is suggested that banding potential for a certain cell type depends on cell cycle time in vitro and the degree of differentiation.