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Association of DEXA with EDX in Evaluation of Inorganic Bone Components After Application of Nano bone For Repair of Bone Defect

Aim: The aim of this study is to correlate DEXA and EDX in assessment the varia-tions in chemical composition in terms of the main elements comprising the bone mineral {calcium (Ca), and phosphorus (P)} after application of Nano bone for Repair of bone defect. Subjects and Methods: A bone drill defect was created in the right tibia (two holes in each tibia) of 48 rabbits; upper hole was left empty while the lower hole was packed with NanoBone. 24 animals were sacrificed 2 weeks after surgery; the remaining animals were sacrificed after 4 weeks. Half of the bone specimens prepared for the routine laboratory processing, histological and histomorphometric, while The remaining specimens were scanned by Dual-energy X-ray absorptiometry (DEXA) for bone mineral density measurement and then used for bone mineral composition determination by Energy Dispersive X-ray Analyses (EDX). The measurements of DEXA and EDX have been compared after that, data were submitted to ANOVA and Tukey’s test. Results: DEXA showed a strong positive correlation with EDX and histological analysis. Conclusion: Energy-dispersive X-ray microanalysis (EDX) is a useful quan-titative tool for the analysis of Ca and P in bone in a non-invasive way. non-collag-enous proteins and proteoglycans account for a small total weight of organic component, though they still have an important role in osteo-blast differentiation and tissue mineralization (2) . The mineralized phase of the bone is composed of hydroxyl- apatite [Ca,o (PO4)6(OH):] in