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Identification of urinary markers using proteomics analysis of urine in patients with bladder cancer.

14605 Background: The discovery of new urinary markers for non-invasive detection and surveillance of bladder carcinoma remains an active field of current research with an aim to replace invasive investigations such as cystoscopy. We have analysed the urinary proteomes of patients with bladder tumor and compared them the urinary proteomes of a healthy volunteer control group to detect putative, tumour specific urinary markers for bladder tumor.


METHODS
24 hour and midstream samples were collected from healthy volunteers and patients with newly diagnosed bladder tumours. Urinary proteins were isolated by a technique previously described by our group (Nabi G, N'Dow J, Hasan ST, Boot IR, Cash P. Proteomic analysis of urine in patients with intestinal segments transposed into the urinary tract. Proteomics. 2005 Apr;5(6):1729-33.)1. Proteins were resolved by 1-dimenional (1-DE) and two dimensional gel electrophoresis (2-DE). The proteins that differed when analysed by either 1-DE or 2-DE between patients and controls were identified using peptide mass mapping.


RESULTS
Six patients with newly diagnosed bladder cancer and 5 healthy volunteers were recruited into this study. All patients were found to have superficial transitional cell carcinoma with high grade (3 with grade 3, 3 with grade 2) on subsequent histopathological examination. For the same individual no detectable differences were observed in the protein profiles of midstream and 24 hour urinary samples when analysed by either 1DE or 2-DE. However, between patient and controls a number of proteins differed in abundance and these were identified. Proteins that were present in patients, but absent in controls, included FK506 binding protein 6 isoform, apolipoprotein A-IV, paraliprotein and lipid binding protein.


CONCLUSIONS
The present study identified some of the novel urinary biomarkers in present with bladder cancer. Some of these have been shown to change their expression with deeper invasion of disease and might be useful in predicting behaviour of superficial bladder cancer. No significant financial relationships to disclose.