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Olive mill wastewater extract as a potential mosquito larvicide

The larvicidal potential of olive mill waste water (OMWW) extract against mosquito larvae was evaluated. We exposed 2nd and 4th instar Culex laticinctus (Edwards) larvae to increasing OMWW concentrations. In addition, the effects of sublethal OMWW concentrations on larval development time and adult size were tested as well. The larvicidal activity of OMWW extract showed LC50 values of 10.08 and 50.07 parts per thousand (ppt) against the 2nd and 4th instars respectively. Larvae that developed in 1 ppt OMWW solution showed sex-specific responses. Males prolonged time to pupation, while females exhibited size reduction in comparison to controls. These results show that OMWW, which is known as agricultural byproduct waste, may be reused as a biopesticide.