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The effect of stirring of the luminal solution on the protection of the rat intestinal mucosa during ischemic injury.

Segments of rat ileum made ischemic for 15 min by occlusion of their mesenteric vessels were intraluminally perfused at slow and rapid rates with Krebs buffer containing O2, O2 + glucose or nitrogen. Mucosal leucine influx was measured at the end of the ischemic period. O2 and O2 with glucose provided protection against the ischemic injury, particularly when the stirring of the luminal solution was increased. Rapid perfusion in the absence of oxygen or glucose also protected the intestine probably by rinsing the ischemic gut of noxious agents.