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Immunolocalization Of Phosphodiesterase Isoenzymes In Rat Tissues Using Confocal Microscopy

Cyclic nucleotide phosphodiesterases (PDE) are a family of structurally related enzymes responsible for degrading cAMP and cGMP in eukaryotic cells[1]. Many tissues express members of multiple PDE families[2]. PDE type 3 (PDE3) has two known isoforms and has been purified from a number of tissues[3]. One of its known functions is relaxation of vascular smooth muscle. PDE type 4 (PDE4) has four known isoforms, has been purified from tissues including germ cells and vascular smooth muscle, and is believed to play a role in blocking contraction of airway smooth muscle[4]. PDE4 inhibitors are among several drug classes that induce segmental medial necrosis of mesenteric arteries after administration to rats. The nature and distribution of the lesions suggest a consequence of extreme localized disturbance of smooth muscle tone. We hypothesized that regional distribution of PDE3 and PDE4 isoenzymes predisposes the rat mesenteric artery to damage; therefore, we explored the distribution of these isoenzymes in a panel of tissues that may be implicated in these drug interactions.