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The modified proteins in erythrocytes and regulation of erythrocytes volume in patients with chronic kidney disease.

OBJECTIVE
The role of oxidatively modified proteins in progression of chronic kidney disease has been discussed. We have got the results demonstrating the alteration of band 3 protein activity in erythrocytes of patients with chronic kidney disease. We presumed that it might be associated with oxidative damage of intracellular proteins. The purpose of the research was to study the modified proteins (protein reactive carbonyl derivatives, membrane-bounded hemoglobin) in erythrocytes, as well as the regulation of erythrocyte volume in patients with chronic kidney disease.


PATIENTS AND METHODS
132 patients with various stages of chronic kidney disease and degree of chronic renal failure were divided into four groups. We enrolled 32 healthy subjects. In erythrocytes modified proteins (protein reactive carbonyl derivatives, membrane-bounded hemoglobin) concentrations and activity of Cl-/HCО3--exchanger have been estimated.


RESULTS
the results demonstrated the strong disorder of Cl-/HCО3--exchanger activity in erythrocytes of patients. These data suggested the existence of erythrocytes subpopulations with different activity of Cl-/HCО3--exchangers in bloodstream of patients with chronic kidney disease depending on initial clinical form of the disease. In erythrocytes of all patients, the membrane-bounded hemoglobin concentration and reactive carbonyl derivatives of proteins were significantly higher than in control samples.


CONCLUSIONS
We have assumed that in erythrocytes oxidized hemoglobin interacts with band 3 protein present on erythrocyte membrane. The membrane-bounded hemoglobin increase leads to increased stiffness of the erythrocyte membranes and affects the volume of erythrocytes. We hypothesized that erythrocytes with changed ability to regulate their volume and high concentration of modified proteins contributed to chronic kidney disease progression.