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Determination of Acetaminophen and Phenacetin in Porcine Microsomal Samples

Abstract A simple, quick, and sensitive high performance liquid chromatography (HPLC) analysis of phenacetin and acetaminophen in porcine hepatic microsome samples is described. Chromatography was performed on a YMC‐Pack ODS‐AQ column using a gradient mobile phase consisting of 0.05% phosphoric acid (pH 3):methanol. UV detection was measured at 254 nm. The procedure produced a linear curve for the concentration range 10–1500 ng/mL and had a limit of detection of 5 ng/mL for both compounds. This assay produced accurate and repeatable quantification of acetaminophen and phenacetin.