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Continuos IBE fermentation by immobilized growing Clostridium beijerinckii cells in a stirred‐tank fermentor

The potential of continuous isopropanol–butanol–ethanol (IBE) fermentation by Ca–alginate‐immobilized Clostridium beijerinckii cells in a continuous stirred‐tank reactor is investigated. A mathematical model is presented to describe steady‐state reactor performance. It appeared to be possible to use the biocatalyst particles repeatedly for successive fermentations (at least three times for a total duration of two months). Reactor productivity was 6–16 times higher than that of a batch fermentation (free cells), while the solvents yield was also increased. Measurements of substrate, product, and biomass concentrations were only partially in agreement with the model; however, a solid basis for further technological developement of the process has been laid.