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UV‐Guided Isolation of Fungal Metabolites by HSCCC

Abstract Analytical standardised reversed phase liquid chromatography (RPLC) data can be helpful in finding a suitable solvent combination for isolation of fungal metabolites by high‐speed counter current chromatography. Analysis of the distribution coefficient (K D) of fungal metabolites in a series of biphasic systems consisting of n‐heptane–ethyl acetate (EtOAc)–methanol (MeOH)–water has been used to illustrate the relationship between the RPLC retention indexes and K D's. The relationship can be used to select a suitable solvent combination for HSCCC, as demonstrated by the isolation of two new cyclic peptides from a crude fungal extract.