{'fraction_non_alphanumeric': 0.014705882352941176, 'fraction_numerical': 0.00392156862745098, 'mean_word_length': 5.673202614379085, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
A simple and efficient method for studying neurotransmitter release in vitro by a radiotracer technique.

A method which allows measurement of neurotransmitter release from isolated preparations has been developed. With this method release of 3H-acetylcholine from guinea pig ileum and rabbit jejunum could be studied after preincubation of the preparations with 3H-choline. The release of noradrenaline from guinea pig vas deferens could also be measured after preincubation with 3H-noradrenaline. The method is based on a device which allows experiments on 4 preparations at the same time, including incubation, field stimulation and collection of fractions for counting of radioactivity. With the purpose of obtaining a simple and inexpensive device for stimulating the tissue preparations electrically, a physiological stimulator distributor (PSD) was constructed. It is concluded that the described method constitutes an inexpensive simple and quick means of studying neurotransmitter release from isolated tissues.