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Competitive Inhibition of Mammalian Hyaluronidase by Tomato Saponin, Esculeoside A

Esculeoside A, a glycoside of spirosolane-type, is identified as a major component in ripe tomato fruits. Our previous study showed that esculeoside A inhibited hyaluronidase activity in vitro and ameliorated experimental dermatitis in vivo. The aim of this present study is to investigate the inhibition mode on mammalian hyaluronidase by esculeoside A. Measured by a modified Morgan-Elson method, the present kinetic analysis of the hydrolysis reaction using hyaluronic acid revealed that no significant difference was observed in velocity maximum Vmax, and Michaelis-Menten constant Km was shown as 0.74 mM in the absence of esculeoside A, was increased as 1.32 and 1.98 mM with 3 and 30 μM of esculeoside A, respectively. Thus suggested that the inhibition mode on hyaluronidase by esculeoside A was competitive. This competitive inhibition on hyaluronidase activity may become valuable in the amelioration of mice experimental dermatitis by esculeoside A.