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Clinical optical dose measurement for PDT: invasive and noninvasive techniques

Two methods for clinical optical light dosimetry are developed. In the first method, which is invasive, a fluorescent probe attached to an optical fiber is inserted by means of a thin hypodermic needle and measures light transmitted through the cheek as a function of the penetration depth. In the second noninvasive method, the diffusely reflected light intensity, from a small illuminated spot on the surface of the tissue to be investigated, is measured as a function of the radial distance along the surface. Preliminary results with both methods are presented. Simulations of the second measurements, which allow for a simplified extraction procedure of the relevant optical data from such measurements, are also shown.