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[The adherence of leukocytes mediated by L-selectin to endothelia activated by burn sera].

OBJECTIVE
To study the mediating effects of L-selectin on the adherence of rat PMNs to the endothelia activated by burn sera.


METHODS
Pulmonary microvascular endothelial cells (PMEC) of rat were cultured by tissue block method. Rat PMNs were harvested by continuous perfusion. The samples were collected for the detection of the adhering rate of PMEC to the PMNs after being cultured with L-selectin monoclonal antibody (mAb), L-selectin ligand and sulfatide, respectively.


RESULTS
The adhering rate of PMNs to PMEC was obviously lowered by the adding of L-selectin mAb and sulfatide.


CONCLUSION
The adherence of PMNs to activated PMEC could be mediated by L-selectin.