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Development of Polyclonal and Monoclonal Antibodies to Iodinated Contrast Media

Two independent systems were developed to produce antibodies to diatrizoate. In the rabbit model polyclonal antibodies were produced that showed cross-reactivity to certain other contrast agents, analogues and serum proteins. In the mouse model monoclonal antibodies were produced that reacted only with diatrizoate, metrizamide, and ovalbumin. These studies demonstrated the potential antigenicity of contrast medium.