{'fraction_non_alphanumeric': 0.035014836795252226, 'fraction_numerical': 0.004154302670623145, 'mean_word_length': 5.771084337349397, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Biochemical Changes in the Platelet Energy Transferring System during Concomitant Action of Cephalin and Serotonin

Summary. The paper concerns the investigations of the changes in the concentrations of endogenous nicotinamide adenine nucleotides (NAD+, NADH, NADP+ and NADPH) and reduced glutathione (GSH) during the incubation of canine blood platelets with cephalin and/or serotonin. Activity of NADH‐dependent glutathione reductase in this instance was also determined. These investigations have revealed the following findings: (1) platelets maintained constant levels of nicotinamide adenine nucleotides and GSH during incubation in Hanks’ solution; (2) 5‐HT induced a marked immediate increase in the amounts of NAD+, NADH and GSH and a moderate reduction in the amount of NADPH; no significant change was seen in the amount of NADP+ or in the activity of NADH‐dependent glutathione reductase; (3) cephalin had similar effects on NAD+, NADH and GSH, but to a lesser extent; in addition, it induced a significant decrease in the amounts of NADP+ and NADPH and a marked activation of NADH‐dependent glutathione reductase; (4) cephalin in addition to 5‐HT amplified these modifications, except for GSH increase (despite an important cephalin‐induced activation of NADH‐dependent glutathione reductase) and produced a marked fall of NADP+ and NADPH. These findings suggest that: (a) platelet nicotinamide adenine nucleotides and GSH exhibit a high sensitivity to these different substances (a phosphatide and an amine) with implications for platelet function and aggregation; (b) cephalin and 5‐HT, despite their different chemical nature, act somewhat similarly on these compounds.