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Development of an Enzyme-linked Immunosorbent Assay to Detect Chicken Parvovirus-specific Antibodies

Important Findings In this study, a specific antibody response of infected chickens was observed starting with IgM production between 14 and 21 days postinfection (DPI) and switching into a predominant IgG response by 32 DPI. The enzyme-linked immunosorbent assay (ELISA) was 93.3% sensitive and 100% specific in detecting chicken parvovirus (ChPV)-infected birds. Subsequent assays identified IgG type ChPVspecific maternally-acquired antibodies in day-old chickens and demonstrated the production of virus-specific antibodies in young birds following infection with ChPV.