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Immunogenic capacity of macrophage hybridomas

Two clones, E2‐7.7 and E2‐10.50, derived from two macrophage(MΨ)hybridomas, E2‐7 and E2‐10, have been studied. The first clone, E2‐7.7, is Ia and Fc receptor (FcR) negative and manifests a strong antigen‐presenting capacity. When we pulsed its cells in vitro with keyhole limpet hemocyanin (KLH) antigen and injected them into syngeneic animals, we found that as small a dose as 103 cells initiated an immune response in vivo. On the other hand, antigen‐pulsed cells of the E2‐10.50 clone, which are Ia‐ and FcR, were almost incapable of triggering immunity, even when injected at a dose of 105 cells. Thus, the two clones differ in their immunogenic capacity (both cellular and humoral immunity). In experiments aimed at testing the stimulation in vitro of primed lymph node (LN) cells by antigen‐pulsed cells of these two hybridoma clones, we observed that E2‐7.7 stimulated the unfractionated population of LN cells and the LN‐derived population of T cells. The E2‐10.50 cells stimulated only the unfractionated population of LN cells, but not the T cell population. Subsequent tests indicated that the E2‐10.50 cells require an intermediate Ia accessory cell to present the antigen to the T lymphocytes.