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Variations in the urinary excretion of purine derivatives and creatinine in holstein/friesan dairy cattle

The urinary excretion of purine derivatives has been proposed as a non-invasive method of estimating the outflow of rumen microbial protein. Wider application of this technique is hindered by the requirement for a total urine collection, but Chen et al. (1992) suggested the use of urinary Creatinine(C) concentration to account for changes in urine volume. The relative concentrations of Purine Derivatives (PD) and C in spot urine samples could be used as a suitable index of PD output. The following experiment assesses the validity of this approach in lactating Holstein/Friesan cattle.