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Influence of HCMV Infection on Expressions of MCM2 and MCM8 in Human Embryonic Lung Fibroblastic Cells

Objective To investigate the influence of HCMV infection on expressions of MCM2 and MCM8 in human embryonic lung fibroblastic cells(HEL),and explore the possible pathogenesis of HCMV infection.Methods Synchronized HEL cells were infected by HCMV,simultaneously set up the mock-infected group as control group.Infected HEL cells were harvested at 12,24,48,72 and 96 hours post infection(h p.i.).The expression levels of MCM2 and MCM8 mRNA in HEL cell were detected with semi-quantitative RT-PCR method.Results The statistical analysis showed the expression levels of MCM2 and MCM8 mRNA in HCMV-infected group were statistically lower than the ones in the mock-infected group at 12,24 h p.i.(P0.05),but at 48,72 and 96 h p.i.,the expression levels of MCM2 and MCM8 mRNA in HCMV-infected group were statistically higher than the ones in the mock-infected group(P0.05);The graphs of the expression levels of MCM2 and MCM8 mRNA suggested that the expression levels reached a peak at 24 h p.i.in the mock-infected group,but in HCMV-infected group reached a peak at 48 h p.i.,which markedly lagged behind the ones in the mock-infected group.Conclusion HCMV infection could induce the abnormal expressions of MCM2 and MCM8 mRNA,and delays their expressions.It is presumably one of the multiple pathways by which the virus blocks the host cell cycle progression.