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Conjugation of E.coli antibody with fluorescent natural silica-based nanoparticles: Preparation and characterization

Silica has entrenched surface chemistry which allows for the surface functionalization with various functional groups, including those of biomolecules. In this study, the aim is to modify fluorescent natural silica nanoparticles (FSNP) with E.coli antibody. The precursor for fluorescent natural silica-based nanoparticles (FSNP) was obtained from a silica precipitate of geothermal power plant in Central Java, Indonesia. This offers a more cost-effective and environmentally friendly route towards the preparation of such nanomaterials. FSNP was synthesized using the sol-gel method to produce impurity-free fluorescent nanoparticles, then activated the groups to conjugate with E. coli antibodies. The surface functionality of the amines is obtained by reacting FSNP with an aminosilane crosslinker (APTES). To produce aldehyde functionality, it is further treated with glutaraldehyde. These aldehyde groups react with the amine groups of biomolecules such as antibodies to form amine bonds. Based on the BraunerEmmett-Teller (BET) analysis, the average surface area is 134.32 m2/g FTIR results showed the formation of NH2 groups after modification of FSNP with APTES at a wavelength of 1635 cm−1. The intensity of FSNP modified FSNP and APTES measured using fluorescence spectroscopy at the FSNP intensity was 688,430 a.u while the intensity of the APTES modified FSNP was 450,474 a.u. The approach of conjugating E.coli antibodies to fluorescence natural silica-based nanoparticles via the APTES crosslinker opens up the possibility of future use of the system as a biosensing platform, particularly the detection of E. coli.