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Duplex and triple helix formation with dA19 and dT19: thermodynamic parameters from calorimetric, NMR, and circular dichroism studies

Differential scanning calorimetric (DSC), NMR, and circular dichroism studies (CD) have been performed on solutions with dA 19 and dT 19 at 1:1 and 1:2 mole ratios at different [NaCl] in PIPES buffer (pH=7.0). An asymmetry exists in the transition regions for the excess heat capacity curves for the dA 19 /dT 19 mole ratio of 1:1 that increases with increasing [NaCl]. In a 1:2 mature of dA 19 and dT 19 with [NaCl] >1.0 M the transition region is much narrower, and the total ΔH is about 50% larger. The CD and NMR spectra for the 1:1 mixture at 0 o C have the same features reported for poly(dA) * poly(dT), confirming the existence of a tight duplex in 1:1 mixtures of dA 19 and dT 19 at low temperatures