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Conjugates of Iron-Transporting N-Hydroxylactams with Ciprofloxacin

Screening of a library of novel N-hydroxylactams amenable by the Castagnoli-Cushman reaction identified four lead compounds that facilitated 55Fe transport into P. aeruginosa cells (one of these synthetic siderophores was found to be as efficient at promoting iron uptake as the natural siderophores pyoverdine, pyochelin or enterobactin). Conjugates of the four lead siderophores with ciprofloxacin were tested for antibacterial activity against P. aeruginosa POA1 (wild type) and the ∆pvdF∆pchA mutant strain. The antibacterial activity was found to be pronounced against the ∆pvdF∆pchA mutant strain grown in CAA medium but not for the POA1 strain. This may be indicative of these compounds being ‘Trojan horse’ antibiotics. Further scrutiny of the mechanism of the antibacterial action of the newly developed conjugates is warranted.


Introduction
Cyclic hydroxamic acid (N-hydroxylactam) moieties are frequently encountered in natural products and synthetic inhibitors of therapeutically relevant enzyme biotargets. The biological activity of N-hydroxylactams often has to do with their ability to chelate metal ions. Chelation of iron is the principal mechanism of action of microbial siderophores (metabolites excreted by iron-starved procaryotic microorganisms into extracellular space to scavenge iron needed for such processes as growth and differentiation [1,2]), examples of which include oxachelin (I) [3], scabichelin (II) [4] as well as structurally simple N-hydroxy-3,4-dihydroisoquinolin-1-one (III) which was isolated from Streptomyces griseus [5]. Anchoring to the prosthetic metal ion is central to the activity of small-molecule N-hydroxylactam inhibitors of such important zinc enzyme targets as matrix metalloprotease (e.g., IV [6] and V [7]), HIV integrase (e.g., VI [8]), histone deacetylases (e.g., VII [9]) as well as of (non-heme) iron-containing enzyme lipoxygenase (e.g., VIII or BMD 188 [10]) ( Figure 1). Some biological effects of N-hydroxylactams manifest themselves independently of metal chelation. Indeed, bacterial siderophores have been shown to possess a signaling function, antibacterial activity and to regulate oxidative stress [11]. At the same time, iron-loaded bacterial siderophores and their synthetic analogs were dubbed 'Trojan horse' carriers for antibiotics and fluorescent labels [12,13]. Conjugation of antibiotics or a fluorescent drugs otherwise eliminated by efflux-mediated resistance mechanisms to a siderophore molecule is a promising approach to circumventing drug resistance as well as to in vivo imaging and eradication of bacterial colonies [14]. This approach received clinical proofof-concept in the 2019 approval of cefiderocol (Fetroja) for the treatment of complicated urinary tract infections [15].
Recently, we developed a facile access to a novel type of N-hydroxylactams (cyclic hydroxamic acids)-trans-2-hydroxy-1-oxo-3-aryl-1,2,3,4-tetrahydroisoquinoline-4-carboxylic acids 1-via a two-component condensation of oximes with homophthalic anhydride (2) [16,17] or via a three-component condensation of aromatic aldehydes, hydroxylamine acetate and homophthalic acid (3) with azeotropic removal of water [18]. In addition to twenty-six hydroxamic acids 1a-z prepared in the course of this investigation (for structures-see Supplementary Materials), we have recently prepared 2-hydroxy-3-(2hydroxyphenyl)-1-oxo-1,2,3,4-tetrahydroisoquinoline-4-carboxylic acid (4) as a compound capable of providing an additional phenolic hydroxy group for metal chelation (Scheme 1). Some biological effects of N-hydroxylactams manifest themselves independently of metal chelation. Indeed, bacterial siderophores have been shown to possess a signaling function, antibacterial activity and to regulate oxidative stress [11]. At the same time, ironloaded bacterial siderophores and their synthetic analogs were dubbed 'Trojan horse' carriers for antibiotics and fluorescent labels [12,13]. Conjugation of antibiotics or a fluorescent drugs otherwise eliminated by efflux-mediated resistance mechanisms to a siderophore molecule is a promising approach to circumventing drug resistance as well as to in vivo imaging and eradication of bacterial colonies [14]. This approach received clinical proof-of-concept in the 2019 approval of cefiderocol (Fetroja) for the treatment of complicated urinary tract infections [15].
Having amassed the library of novel N-hydroxylactams 1a-z and 4, we became interested in identifying, via screening, non-cytotoxic lead compounds capable of transporting iron into Gram-negative Pseudomonas aeruginosa bacterial cells. We reasoned that, considering that these compounds contain a conveniently positioned carboxylic acid functionality, the newly identified lead iron-complexing-and iron-transporting-compounds could be conjugated to a known antibiotic molecule (e.g., ciprofloxacin as was recently described [19,20]). Herein, we report our results obtained in the course of realization of this strategy.

Results and Discussion
In order to establish if P. aeruginosa cells are able to use any of the synthesized compounds 1a-z or 4 to access extracellular iron, we used a growth assay in iron-restricted conditions, i. e. using iron-deficient casamino acid (CAA) medium and iron concentration around 20 nM, according to the previous work [21]. The P. aeruginosa ∆pvdF∆pchA mutant unable to produce the siderophores pyoverdine [22] and pyochelin [23] was used [24] and was grown on a CAA medium in the absence or presence of 10 mM and 100 mM concentration of the compounds screened. This bacterial strain is able to grow in an iron-deficient environment likely due to citrate acting as siderophore [25] or owing to the functioning of the iron reduction systems which import ferrous ions through the feoABC system [26]. The assay is based on the premise that in the presence of an excess of a metal chelator, all iron traces present in the growth medium become scavenged, making this nutrient no longer available for low-affinity iron import systems. However, if the growth of the P. aeruginosa ∆pvdF∆pchA mutant is still observed in the presence of the tested compounds, it would be indicative of the bacteria being able to use these iron chelators as siderophores.
A large portion of compounds 1a-z and 4 tested displayed nearly total growth inhibition at 10 µM, indicating that such compounds cannot be used by P. aeruginosa as siderophores to access iron. For 12 compounds tested (1a, 1c-e, 1i-k, 1t-v, 1x, 1z, 4), the results were more promising, with a total inhibition at 100 µM and no or a partial inhibition at 10 µM (see Figure S1, Supplementary Materials). These compounds were then screened for the ability to transport 55 Fe into P. aeruginosa cells.
The compounds were loaded with 55 Fe and incubated with the P. aeruginosa ∆pvdF∆pchA mutant. The latter was chosen so as to avoid any uptake of 55 Fe via the endogenous siderophores (pyoverdine and pyochelin). Reassuringly, for compounds 1a (DVD-000265), 1c (DVD-00627) and 4 (DVD-000468), an uptake of around 40-70 pmol of 55 Fe/mL/OD 600 nm after 30 min was observed and only in the absence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) [27]. Because CCCP had been shown to inhibit any iron uptake by TonB-dependent transporters (bacterial outer membrane proteins that bind and transport siderophores [28]) in bacteria [29,30], this finding indicates that the uptake of iron by these compounds was not due to passive diffusion via porins [31], but rather due to a proton motive force-dependent transport relying on TonB. A special case was identified for compound 1i (DVD-000304), as an uptake of about 150 pmol 55 Fe/mL/OD 600 nm was observed after 30 min incubation and was also abolished in the presence of CCCP indicating it being a TonB-dependent uptake. Such a high 55 Fe uptake by compound 1i was judged to be as efficient as the one observed for natural siderophores pyoverdine, pyochelin or enterobactin in P. aeruginosa [30]. Altogether, these data show an iron uptake into P. aeruginosa cells in the presence of compounds 1a (DVD-000265), 1c (DVD-00627), 1i (DVD-000304 and 4 (DVD-000468), which suggests that these compounds can be used as siderophores by this pathogen (Figure 2).
Having identified the four lead synthetic siderophores (1a, 1c, 1i and 4), all of which contain a carboxylic acid functionality suitable for conjugation, we proceeded to synthesize the 'Trojan horse'-type conjugates of these siderophores with antibiotic ciprofloxacin. To this end, we synthesized three N-benzyloxylactams 5a-c as depicted in Scheme 2.
A special case was identified for compound 1i (DVD-000304), as an uptake of about 150 pmol 55 Fe/mL/OD600 nm was observed after 30 min incubation and was also abolished in the presence of CCCP indicating it being a TonB-dependent uptake. Such a high 55 Fe uptake by compound 1i was judged to be as efficient as the one observed for natural siderophores pyoverdine, pyochelin or enterobactin in P. aeruginosa [30]. Altogether, these data show an iron uptake into P. aeruginosa cells in the presence of compounds 1a (DVD-000265), 1c (DVD-00627), 1i (DVD-000304 and 4 (DVD-000468), which suggests that these compounds can be used as siderophores by this pathogen (Figure 2). (DVD-000468) in P. aeruginosa cells; structures of the four lead siderophores; P. aeruginosa pyoverdine and pyochelin deficient strain (∆pvdF∆pchA) was grown in CAA medium overnight as described in Materials and Methods. Afterwards, cells were washed with 50 mM Tris-HCl pH 8.0 and re-suspended in the same buffer to and OD 600 nm (optical density measured at 600 nm) of 1. The iron transport assay was started by the addition of 500 nM 55 Fe-loaded compounds 1a, 1c, 1i and 4 and, at different time points, aliquots were taken, bacteria were pelleted and the radioactivity retained in the cells was monitored. The ∆pvdF∆pchA strain was also treated with 200 mM of CCCP protonophore and incubated in the presence of 55 Fe-loaded compounds (CCCP inhibits any proton motive dependent uptake). tive dependent uptake).
Having identified the four lead synthetic siderophores (1a, 1c, 1i and 4), all of whic contain a carboxylic acid functionality suitable for conjugation, we proceeded to synthe size the 'Trojan horse'-type conjugates of these siderophores with antibiotic ciprofloxacin To this end, we synthesized three N-benzyloxylactams 5a-c as depicted in Scheme 2.

Scheme 2. Synthesis of N-benzyloxylactams 5a-c.
Compounds 5a-c were amidated using HATU and methyl 4-aminobutanoate [32 Following basic hydrolysis, carboxylic acid building blocks 6a-c were obtained in exce lent yields over two steps. HATU-mediated coupling with ciprofloxacin methyl or benzy ester gave compounds 7a-c (in case of 7c, the benzyl ester group underwent hydrolysi during the HPLC purification). Removal of the benzyl group from the N-benzyloxy lactam moiety and ester hydrolysis of intermediate 8a' gave compound 8a. Treatment of com pound 7b with BBr3 led to the simultaneous deprotection of the N-hydroxylactam, th ester and the phenolic hydroxy group to give compound 8b. Hydrogenation of 7b and 7 gave siderophore-ciprofloxacin conjugates 8c and 8d, respectively (Scheme 3).
Siderophore-ciprofloxacin conjugates 8a-d were tested for antibacterial activit against P. aeruginosa PAO1 (wild type) strain and the ∆pvdF∆pchA mutant. Since the latte are not able to produce pyoverdine and pyochelin, they may be able to more efficientl use the synthetic siderophore-ciprofloxacin conjugates to get access to iron compared t the PAO1 strain. The minimum inhibitory concentrations (MIC) were first determined fo both strains grown in Müller-Hinton (MH) medium [33]. For both strains, no antibacteria activity was detected, which is not surprising for bacteria grown in MH medium since th outer membrane transporters involved in iron acquisition are only expressed under iron restricted growth conditions [30]. Consequently, we also determined the MIC values i iron-restricted conditions (CAA medium). Again, no antibiotic activity was observe against the PAO1 strain. However, for the ∆pvdF∆pchA mutant, MICs of 1 mM were de termined for all four siderophore-ciprofloxacin conjugates (Table 1).

Scheme 2. Synthesis of N-benzyloxylactams 5a-c.
Compounds 5a-c were amidated using HATU and methyl 4-aminobutanoate [32]. Following basic hydrolysis, carboxylic acid building blocks 6a-c were obtained in excellent yields over two steps. HATU-mediated coupling with ciprofloxacin methyl or benzyl ester gave compounds 7a-c (in case of 7c, the benzyl ester group underwent hydrolysis during the HPLC purification). Removal of the benzyl group from the N-benzyloxy lactam moiety and ester hydrolysis of intermediate 8a' gave compound 8a. Treatment of compound 7b with BBr 3 led to the simultaneous deprotection of the N-hydroxylactam, the ester and the phenolic hydroxy group to give compound 8b. Hydrogenation of 7b and 7c gave siderophore-ciprofloxacin conjugates 8c and 8d, respectively (Scheme 3).  Siderophore-ciprofloxacin conjugates 8a-d were tested for antibacterial activity against P. aeruginosa PAO1 (wild type) strain and the ∆pvdF∆pchA mutant. Since the latter are not able to produce pyoverdine and pyochelin, they may be able to more efficiently use the synthetic siderophore-ciprofloxacin conjugates to get access to iron compared to the PAO1 strain. The minimum inhibitory concentrations (MIC) were first determined for both strains grown in Müller-Hinton (MH) medium [33]. For both strains, no antibacterial activity was detected, which is not surprising for bacteria grown in MH medium since the outer membrane transporters involved in iron acquisition are only expressed under iron-restricted growth conditions [30]. Consequently, we also determined the MIC values in iron-restricted conditions (CAA medium). Again, no antibiotic activity was observed against the PAO1 strain. However, for the ∆pvdF∆pchA mutant, MICs of 1 mM were determined for all four siderophore-ciprofloxacin conjugates ( Table 1). Table 1. MIC values determined for ciprofloxacin-siderophore conjugates 8a-d against P. aeruginosa PAO1 and ∆pvdF∆pchA strains grown in MH or CAA (iron-restricted) medium (both strains were grown in the presence of increasing concentrations of 8a-d; the MIC values were measured in µM after 48 h in triplicates; ciprofloxacin was used as the positive control displaying an MIC value of 48 ± 1.2 µM).
The observed antibacterial effect from siderophore-ciprofloxacin conjugates 8a-d solely on the P. aeruginosa ∆pvdF∆pchA mutant strain grown in (iron-restricted) medium may be indicative of these conjugates indeed acting as 'Trojan horse' antibiotics. The iron-starved bacteria unable to produce and excrete their own siderophores (pyoverdine and pyochelin) are likely to utilize the siderophoric constructs 8a-d to shuttle the iron inside the cells. Once inside the bacteria, these constructs may already exert their antibiotic activity due to the presence of a ciprofloxacin payload. Unfortunately, we were unable to monitor the 55 Fe uptake with these compounds because of the solubility problems encountered.

Bacterial Strains and Growth Assays in Iron-Restricted Conditions
The P. aeruginosa PAO1 and ∆pvdF∆pchA strains were used in this study. PAO1 is a wild type strain and ∆pvdF∆pchA is a strain unable to produce the siderophores pyoverdine and pyochelin [22]. Bacteria were first grown in LB medium overnight at 30 • C, and then washed and re-suspended in 20 mL iron-deficient CAA (casamino acid) medium containing 5 g·L −1 low-iron CAA (Difco), 1.46 g·L −1 K 2 HPO 4 ·3H 2 O and 0.25 g·L −1 MgSO 4 ·7H 2 O and grown overnight at 30 • C. Bacteria were then washed, re-suspended in CAA medium at an optical density of 0.02 at 600 nm and distributed into the wells of a 96-well plate (Greiner, U-bottomed microplate) in the absence or in the presence of 10 µM or 100 µM of the tested compounds (5 mM stock solutions in DMSO were used). The plate was incubated at 30 • C, with shaking, in a TECAN microplate reader (Infinite M200, Tecan) (Tecan, Männedorf, Switzerland) and bacterial growth was monitored at OD 600 nm . The presented data are the mean of three replicates for each measurement.

Iron Uptake
Compound-55 Fe complexes were prepared at 55 Fe concentrations of 50 µM, with a compound:iron (mol:mol) ratio of 200:1 as described previously [22]. P. aeruginosa strains were successively grown an overnight in LB broth, followed by an overnight in CAA medium and finally overnight in CAA medium. All of these successive cultures were carried out at 30 • C. The bacteria were subsequently used for 55 Fe uptake kinetics as described previously [22], with a concentration of 55 Fe-compound complexes of 500 nM and with cells pretreated or not with 200 µM CCCP (a proton motive force inhibitor) [27].

MIC Determination
Overnight cultures were grown at 37 • C in Lysogeny broth (LB) and diluted to obtain an opacity equivalent to 0.5 on the McFarland scale. Screening vials were filled with solutions of the test compounds in 0.5% DMSO as prepared above with three replications for each treatment. Ciprofloxacin (0.5-256 µg/mL) and 0.5% DMSO served as positive and negative controls, respectively. The entire vial was incubated at 35 ± 2 • C for 18 h. After incubation, the antibacterial activity of the test compounds was determined by measuring the absorption of the solution with a spectrophotometer on 500 nm.
The MICs were measured using the twofold serial broth dilution method. The test organisms were grown in suitable broth for 18 h at 37 • C. Twofold serial dilutions of solutions of the test compounds were prepared at 256, 128, 64, 32, 16, 8, 4, 2, 1 and 0.5 µg ml −1 . The tubes were then inoculated with the test microbe; each 5 mL received 0.1 mL of the above inoculums and were incubated at 37 • C. The vials were subsequently observed for the presence or absence of microbial growth.

4-((3RS,4RS)-2-(Benzyloxy)-3-(2-methoxyphenyl)-1-oxo-1,2,3,4tetrahydroisoquinoline-4-carboxamido)butanoic acid (6b)
This compound was obtained following the same two-step procedure as for compound 6a starting from (3RS,4RS)-2-(benzyloxy)-3-(2-methoxyphenyl)-1-oxo-1,2,3,4-tetrahydroisoquinoline-4-carboxylic acid (5b, 845 mg, 2.1 mmol) in 92% yield (941 mg). 1 66 (m, 2H). 13   To a solution of compound 6a (100 mg, 0.2 mmol), DMF (1 mL) DIPEA (60 mg, 0.47 mmol) and HATU (84 mg, 0.22 mmol) were added at room temperature. After stirring for 5 min, methyl methyl 1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3carboxylate hydrochloride (76 mg, 0.2 mmol) was added and the mixture was left overnight. The reaction mixture was diluted with EtOAc and washed with water. The organic phase was dried over sodium sulfate, filtered and concentrated to give the crude product which was purified by flash column chromatography on silica (mobile phase hex-acetone 5-100%, then acetone-MeOH) to give 113 mg, 67% of pure title compound. 1  To a solution of compound 6b (630 mg, 1.15 mmol) in DCM (20 mL) NEt 3 (0.5 mL) and HATU (480 mg, 1.26 mmol) were added at room temperature. After stirring for 5 min, benzyl 1-cyclopropyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylate (550 mg, 1.31 mmol) was added and the mixture was left stirring overnight. The reaction mixture was diluted with water (30 mL) + conc. HCl (1 mL) and the resulting suspension was filtered. The solid material was washed with water (30 × 3 mL) and ether (20 × 2 mL) and dried in air to give pure title compound. Yield 1.00 g, 98%. 1  Compound 7b (489 mg, 0.55 mmol) was placed in a round-bottom flask equipped with a magnetic stir bar and a dropping funnel capped with a calcium chloride filled drying tube followed by the addition of 100 mL of dry DCM. After cooling to 0 • C a solution of BBr 3 (635 mg, 2.54 mmol) in DCM (50 mL) was added dropwise to the flask within 30 min. After stirring for four days at room temperature the reaction mixture was poured onto 100 mL of crushed ice and stirred for 2h. The resulting solid was filtered, washed with water (10 mL) and THF (5 mL) to give pure title compound. Yield 23 mg, 15%. 1   In a round bottom flask equipped with a rubber septum and magnetic stir bar, compound 7b (100 mg, 0.11 mmol) was dissolved in THF/MeOH mixture (10/30 mL) followed by the addition of Pd/C (10% wt) catalyst (60 mg, 0.5 eq). Hydrogen gas (1 atm) was slowly bubbled through reaction mixture via needle for 4h and then the hydrogen inlet was removed and the reaction mixture was left stirring under atmosphere of hydrogen overnight. The reaction progress was monitored via HPLC. After completion of the reaction the reaction mixture was filtered through a pad of Celite, which was washed with THF (20 mL) and ACN (20 mL). This filtrate contained admixture only and was discarded. Celite was further washed with hot DMF (20 mL) to give 65 mg of crude product, which was purified by precipitation from DCM solution (10 mL) with Et 2 O to give 8c. Yield 30 mg, 38%. 1    In a round bottom flask equipped with a rubber septum and magnetic stir bar, compound 7c (53 mg, 0.07 mmol) was dissolved in MeOH (20 mL) followed by the addition of Pd/C (10% wt) catalyst (50 mg, 0.7 eq). Hydrogen gas (1 atm) was slowly bubbled through the reaction mixture via needle for 4h and then the hydrogen inlet was removed and the reaction mixture was left stirring under atmosphere of hydrogen overnight. The reaction progress was monitored via HPLC. After completion of the reaction the reaction mixture was filtered through a pad of Celite, which was washed with THF (20 mL). The filtrate was concentrated and the residue was purified by preparative RP-HPLC (ACN-water + 0.1% TFA; gradient 20-90% of ACN in 35 min; 40 • C, 12 mL/min) to give 18 mg, 41% of pure title compound as yellow oil. 1

Conclusions
The screening of a library of novel N-hydroxylactams amenable by the Castagnoli-Cushman reaction identified four lead compounds that exerted a minimal effect on the growth of P. aeruginosa ∆pvdF∆pchA mutant strain grown in (iron-restricted) CAA medium and transported 55 Fe into P. aeruginosa cells. Notably, one of these synthetic siderophores was found to be as efficient at promoting iron uptake as the natural siderophores pyoverdine, pyochelin or enterobactin. Conjugates of the four lead siderophores with ciprofloxacin were synthesized via sequential amide coupling and protecting group manipulation. These were tested for antibacterial activity against P. aeruginosa POA1 (wild type) and the ∆pvdF∆pchA mutant strain. For bacteria grown under non-restricted iron conditions (in Müller-Hinton medium), no antibacterial activity was observed. Likewise, none of the siderophore-ciprofloxacin constructs was active against the POA1 strain grown in iron-restricted conditions (CAA medium). However, the antibacterial activity was found to be pronounced against the ∆pvdF∆pchA mutant strain grown in CAA medium. This may be indicative of the uptake of these compounds by the iron-starved bacteria and of their being 'Trojan horse' antibiotics. Further scrutiny of the mechanism of the antibacterial action of the newly developed conjugates is warranted.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/molecules27123910/s1. Structures of the compounds screened. Copies of NMR spectra. Bacterial growth graphs. Figure S1: Growth of ∆pvdF∆pchA P. aeruginosa mutant in the absence or presence of 10 µM and 100 µM of the different compounds in iron-restricted medium. The growth medium used was CAA and growth was followed by monitoring optical density (OD) at 600 nm at 30 • C.