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Carrier‐Mediated Transport of Chloride Across the Blood‐Brain Barrier

Abstract: 36Cl concentrations in each of eight brain regions and in cisternal cerebrospinal fluid (CSF) were determined 30 min after the intravenous injection of 36Cl in dialyzed‐nephrectomized rats with plasma Cl concentrations between 14 and 120 μmol × ml−1. CSF 36Cl exceeded 36Cl concentrations in brain extracellular fluid. The calculated blood‐to‐brain transfer constants for Cl, kCl, ranged from 1.8 × 10−5 s−1 at the parietal cortex to 3.8 × 10−5 s−1 at the thalamus‐hypothalamus. kCl fell by 42–62% when mean plasma [Cl] was elevated from 16 to 114 μmol × ml−1. Brain uptake of [14C]mannitol or of 22Na was independent of plasma [Cl], but 22Na influx into CSF fell when plasma [Cl] was reduced. Cl flux into brain and CSF could be represented by Michaelis‐Menten saturation kinetics, where, for the parietal cortex, Km= 43 μmol × ml−1 and Vmax= 2.5 × 10−3μmol × s−1× g−1, and for CSF Km= 68 μmol × ml−1. At least 80% of 36Cl influx into the parietal cortex was calculated to occur at the cerebrovascular endothelium, whereas the remainder was derived from tracer that first entered CSF. The CSF contribution was greater at brain regions adjacent to cerebral ventricles. The results show that Cl transport at the cerebrovascular endothelium as well as at the choroid plexus epithelium is a saturable concentration‐dependent process, and that the CSF is a significant intermediate pathway for Cl passage from blood to brain.