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Evaluation of cellular behavior in a multilayer structured tubular tissue with the PLCL scaffold

We proposed a 3D assembly technique of small-diameter blood vessels using a PLCL (poly (L-lactide-co-ε-caprolactone)) scaffold. The technique uses a residual stress of PLCL scaffolds to fabricate a multilayer structured tubular tissue, and gives a tissue mechanical property which blood vessels originally have. In the future, we try to test the circulatory culture system in order to investigate whether the tissue-engineered structure maintains the equivalent mechanical property as the human blood vessel. In this work, we demonstrated that fabricated tissues could attach on the inside of tubular PLCL scaffold in the appropriate conditions.