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Method for Making Multitissue Control Blocks

Abstract Multitissue blocks are considered best practice and play a major role in maintaining quality control of immunohistochemical stains. When used as combined positive and negative tissue controls, they can serve as a permanent record documenting the sensitivity and specificity of every immunohistochemical stain, particularly when mounted on the same slide as the patient tissue. If the multitissue block components are chosen appropriately, one multitissue control block can be used to assess the quality of several antibodies (College of American Pathologist Commission on Laboratory Accreditation). This technical note describes a technique to make blocks of multitissue samples bound by placental membrane. (The J Histotechnol 33(1):49–51, 2010) Submitted November 24, 2009; accepted January 30, 2010.