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Physical methods for the analysis of molecular interactions in dyes utilizable in viral biology studies.

The possibilities of the main physical methods for the analysis of molecular interactions in solution are discussed on the ground of recent data of the literature. Spectrophotometry, (micro)cryoscopy and the isopiestic method are presented in more detail. These methods were applied to several dyes of common use in viral biology studies and to some new chromophore structures. The results obtained demonstrate the occurrence of molecular aggregation in solution in several of the above mentioned systems, both upon concentration rises and in the presence of inorganic ions.