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Application of Raman scattering in the analysis of the Elodea canadensis genomic dsDNA at different stages of the plant development

Abstract Raman spectroscopy was used to investigate the shifts of vibrational marker bands of genomic double-stranded DNA (dsDNA) extracted from Elodea canadensis (Michx. 1803) young and senescent leaves that were used as an experimental model. Spectra were obtained using Renishaw inVia Reflex system with NIR 785.0 nm diode laser excitation in the Raman shift range 620–1,720 cm–1. Additionally, the dsDNA samples were analyzed by gel electrophoresis and spectrophotometry. The identified patterns showed that the Raman bands intensity depends on the plant dsDNA quality and condition. Spectra from smeared dsDNA differ from high-molecular dsDNA and thus can be easily identified. However, the full range spectrum intensity of smeared dsDNA was significantly decreased, but some of the high-intensity peaks from dsDNA extracted from young leaves disappeared or their intensities were significantly reduced relative to other samples. Due to the decrease of the total intensity of all peaks, an increase in the number of low-intensity peaks over the entire length of the spectrum was observed.