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Mass spectrometry-based metabolites analysis of two licorice chalcones in mice plasma, bile, feces and urine after oral administration.

Isoliquiritigenin and isoliquiritin, two kinds of major flavonoids in licorice, are biological active substances with antioxidant, anti-inflammatory and tumor-suppressive effects. However, their in vivo metabolites, possible material basis of this two licorice chalcones for the diseases treatment, have not been fully studied. To clarify the metabolism of isoliquiritigenin and isoliquiritin, the metabolites of this two licorice chalcones in mice plasma, urine, feces and bile after oral administration of 100 mg/kg/d for consecutive 8 days were determined using liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry in this study. The structures of those metabolites were tentatively identified according to their fragment pathways, accurate masses, characteristic product ions, metabolism law and reference standards-matching. As a result, a total of 25 o 29 metabolites of isoliquiritigenin or isoliquiritin were respectively identified. Seven main metabolic pathways, including oxidation and reduction, deglycosylation and glycosylation, dehydroxylation and hydroxylation, demethoxylation and methoxylation, acetylation, glucuronidation and sulfation were summarized to tentatively explain how the metabolites were biologically transformed. These results provide the important information on the metabolism of isoliquiritigenin and isoliquiritin, which may be helpful for the further research of their pharmacological mechanism.