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Effects of changes in pH of bath fluid on isolated guinea pig ileum preparation.

The effects of changes in the pH of Tyrode's solution on the responses of isolated guinea pig ileum preparation to acetylcholine and histamine were studied. At higher levels of pH (increased alkalinity), the responses of the tissue to both acetylcholine and histamine were increased. At lower pH levels (increased acidity), the response of the tissue to acetylcholine was increased but that to histamine was reduced.