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Haemoglobin Content of Individual Erythrocytes in Normal and Abnormal Blood

Summary. Procedures were developed for the measurement of the haemoglobin content of individual unstained human erythrocytes in blood smears, using a Vickers M85 scanning and integrating microdensitometer. Possible errors due to instrumental factors were investigated. Spatial distribution error was found to be unimportant when using a flying spot 1.8 μm diameter in the specimen plane. Measurements of an oxyhaemoglobin solution adhered to Bouguer's Law (i.e. absorbance was proportional to pathlength); microdensitometric results obtained at a given wavelength and with a given monochromator bandwidth could be corrected to correspond to λmax and minimal bandwidth by multiplication by an empirical constant.