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[Determination of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth after compatibility with Coptis chinensis Franch].

OBJECTIVE
To studying changes of evodiamine and rutaecarpine in E. rutaecarpa after compatibility with C. chinensis.


METHOD
An YWG C18 column was used with a mobile phase of CH3CN-H2O(40:60), the flow rate was 1.0 ml.min-1 and the detection wavelength was 290 nm.


RESULT
The average recovery was 97.82% and 97.00%, the relative standard deviation was 0.81% and 1.10% for evodiamine and rutaecarpine respectively.


CONCLUSION
The amounts of evodiamine and rutaecarpine were decreased after compatibility with C. chinensis.