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Isolation and identification of two novel SDS‐resistant secreted chitinases from Aeromonas schubertii

Two SDS‐resistant endochitinases, designated as ASCHI53 and ASCHI61, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI‐TOF mass measurement indicates the molecular weights of 53,527 for ASCHI53 and 61,202 for ASCHI61. N‐terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCHI53 and ASCHI61 retained, respectively, 65% and 75%, of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% β‐mercaptoethanol. These results demonstrate that they are SDS‐resistant endochitinases and probably have a rigid structure. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009