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Antibody formation to dog pulp tissue altered by a paste containing paraformaldehyde.

Pulpotomy treatment was performed weekly on the teeth of four dogs for 5 weeks. Antibody formation to pulp tissue altered by paraformaldehyde paste within the pulp cavity was measured by enzyme linked immunosorbent assays (ELISA). The results indicated that the reaction between antigen and antibody did occur when pretreatment or post-treatment serum reacted with the antigen of either the pulp tissue incubated with saline or that incubated with paraformaldehyde paste. No evidence was found to prove that antibody formation was related to the pulpotomy treatment with paraformaldehyde paste.