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A rapid method for the determination of urea stable lactate dehydrogenase on the 'Cobas Bio' centrifugal analyser.

The urea-stable lactate dehydrogenase assay has been adapted to the 'Cobas Bio' centrifugal analyser. It was found that incubation of the serum samples with 2 mol/l urea for 2 min was sufficient for inhibition of the LD liver isoenzymes. Under these conditions enzyme activity of cardiac origin was mainly unaffected. The method is fully automated, rapid and inexpensive.