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Attenuation of Ehrlichia canis by multiple passages in two different cultures.

Canine monocytic ehrlichiosis (CME) is a tickborne disease with global importance. While trying to determine the minimal time-lag needed for transmission of Ehrlichia canis by Rhipicephalus sanguineus, trials to infect ticks by feeding on infected dogs were carried out. The results of the current study suggest that the organism was attenuated during multiple passages in two different cell cultures.