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Observation of Biospecimens at Cryotemperatures with Soft X-ray Microscope

We have been developing a cryogenic system for biospecimens at soft X-ray microscope with a compact SR source. We present here the features of this system and the results obtained for some biospecimens. The microscope used can image specimens surrounded by cold nitrogen gas from liquid nitrogen under atmospheric pressure. The liquid nitrogen flux is controlled by a temperature controller with a platinum resistance thermometer. The specimens can be maintained at the temperature preset within the range of 110–273 K. The cryogenic system has been installed at the X-ray microscopy beamline and the first cryogenic images have been acquired.