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Determination of arylamines in urine without the interference of indican.

Abstract The color response of indican (indoxyl sulfate) in the Bratton-Marshall 6 method for arylamines is compared to the color response of urine, which is presumably due to indican from tryptophan metabolism. A method is presented for the determination of free or substituted arylamines in urine without interference of indican. The positive color of blank urine is eliminated. This method is based on diazotization and coupling of the amine at pH 2.3 in the presence of trichloroacetic acid buffered by sodium citrate.