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Survival and Infectivity of Pneumocystis carinii Outside the Mammalian Host.

SUMMARY Air blower prefilters servicing HEPA‐filtered Biobubble housing Pneumocystis carinii‐infected rats were stored for up to five months at ‐80°C to room temperature. After storage, 76% of immunosuppressed rats exposed to these prefilters developed P. carinii infections. In contrast, only 4% of control immunosuppressed rats exposed to autoclaved filters had P. carinii infections. These observations indicate that the organism has a dormant form that remains infective for at least several months outside the mammalian host.