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Nucleotide sequence of the rpoA gene in wheat chloroplast DNA.

The nucleotide sequence of a wheat (Triticum aestivum cv. Mardler) chloroplast gene (rpoA) encoding a protein homologous to the a subunit of RNA polymerase from Escherichia coli (1) has been determined. The gene is located at the end of the 27.2kbp SalI fragment (Si) nearer the inverted repeat sequence (2), and is largely contained within the 1.7kbp BamHI fragment (B20). The 3' end of the coding region is located 186bp from the 3' end of the petD gene. The gene encodes a protein of 339 amino acid residues. The deduced amino acid sequence contains 29% residues identical to the E. coli protein (1), 47% identical to the Marchantia polymorpha chloroplast protein (3), 69-71% identical to the chloroplast proteins from spinach, tobacco and pea (4-6), and 86% identical to the maize chloroplast protein (7).