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Evaluating the Mitochondrial Activity and Inflammatory State of Dimethyl Sulfoxide Differentiated PLB‐985 Cells

Neutrophils play a key role as the first line of defense against pathogens. Their ability to generate and release inflammatory mediators (eicosanoids or cytokines) helps promote the inflammatory response and consequently restore the hemostasis. While active participants in several steps of the normal inflammatory response, neutrophils are also involved in chronic inflammatory diseases such as asthma, atherosclerosis and arthritis. Given their dual role in the modulation of inflammation, regulating the inflammatory response of neutrophils has been an important therapeutic approach pursued by numerous researchers. The neutrophil's lifespan is relatively short (8–12h), which can be problematic for some in vitro experiments. To address this issue, researchers used the human monomyelocyte cell line PLB‐985 as an alternative for some of their in vitro experiments. PLB‐985 cells can be differentiated into a more neutrophil‐like phenotype upon exposure to several agonists, including dimethyl sulfoxide (DMSO). Whether this differentiation of PLB‐985 affects important features related to neutrophil's normal functions (i.e. mitochondrial activity, eicosanoid production) remains elusive and characterizing these changes will be the focal point of this project.