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HUMAN SENILE CATARACT AND Na‐K ATP'ase ACTIVITY IN THE ANTERIOR LENS STRUCTURES

Location and in vitro determination of Na‐K ATP'ase activity in the anterior structures of individual human lenses with senile cataract are reported, with special reference to anterior capsular/subcapsular opacity (ACSCO). Histochemically, ATP'ase reaction products were found exclusively in the epithelium. Even totally opaque lenses showed strong positive reactions. Biochemically, increasing ratios of Na+/K+ concentrations in the assay medium resulted in an increase in enzyme activity to a limited degree, whereafter the activity remained stable.