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Uncoupling RARA transcriptional activation and degradation clarifies the bases for APL response to therapies

Synthetic retinoids activate RARA- or PML/RARA-dependent transcription, but fail to degrade RARA or PML/RARA protein, which is insufficient for eradication of acute promyelocytic leukemia.


Brief Definitive Report
Acute promyelocytic leukemia (APL) is driven by the PML/RARA fusion protein, which interferes with both transcriptional regulation by RARA and the assembly-and presumably function-of PML nuclear bodies (Lallemand-Breitenbach and de Thé, 2010). RARA signaling controls myeloid differentiation (Kastner et al., 2001), whereas PML is implicated in selfrenewal (Ito et al., 2008), which likely explains how PML/RARA enforces both a promyelocyte differentiation block and acquisition of self-renewal. APL is a unique model in cancer biology as a result of the empirical discovery of two active drugs, retinoic acid (RA) and arsenic trioxide, which have been shown a posteriori to target PML/RARA (Quignon et al., 1997;de Thé and Chen, 2010). RA, which elicits APL cell differentiation ex vivo and in vivo, yields transient remissions in patients or mice. Arsenic initiates delayed differentiation in vivo only, but nonetheless cures up to 70% of de novo patients. Combined treatment with RA and arsenic definitively cures all mice and over 90% of patients (Lallemand-Breitenbach et al., 1999;Rego et al., 2000;Hu et al., 2009;de Thé and Chen, 2010).
Mechanistically, RA converts PML/RARA from a constitutive repressor into a transcriptional activator, which could explain the differentiation block reversal (Melnick and Licht, 1999). In addition, both RA and arsenic trigger PML/RARA degradation by, respectively, targeting each of its RARA and PML constitutive moieties (de Thé and Chen, 2010). Although some correlative studies have suggested that RESULTS AND DISCUSSION Uncoupled retinoids activate RARA signaling but fail to initiate RARA degradation Previous studies had demonstrated that RA-induced RARA catabolism was tightly linked to its ability to activate transcription (Zhu et al., 1999). In an attempt to identify molecules that could modulate RA-triggered RARA degradation, we stably expressed an EGFP-RARA fusion in H1299 cells and examined the ability of 800 FDA-approved drugs to enhance or antagonize RA-induced degradation. Cells were treated with the drugs (10 µM) for 30 min before an overnight RA treatment (1 µM), and nuclear EGFP was quantified on live cells. As expected, some RAR or RXR agonists further reduced EGFP-RARA levels, whereas proteasome inhibitors blocked its RA-induced degradation. Interestingly, multiple drugs with glucocorticoid/progestative activities antagonized RA-induced therapy-induced PML/RARA loss may be responsible for the clearance of leukemia-initiating cells (Nasr et al., 2008), blast differentiation through transcriptional activation of PML/ RARA is still widely considered to account for therapeutic efficacy of RA, so that the molecular bases for this extraordinary clinical success remain disputed. Elucidation of the role of PML/RARA degradation was until now precluded by the lack of nuclear receptor mutants for which transcriptional activation and degradation would be fully uncoupled or of ligands that would activate transcription but fail to degrade the oncogenic fusion. Here, we identify two such molecules and demonstrate that although they trigger efficient APL cell differentiation, they fail to abolish leukemia-initiating activity ex vivo or in vivo. Thus PML/RARA degradation underlies RA anti-leukemic activity, unifying the modes of action of RA and arsenic.

Br ief Definitive Repor t
progenitors, which exhibit a promyelocytic differentiation block and can be serially replated in methylcellulose cultures (Du et al., 1999;Zhu et al., 2005). As previously reported, RA induced terminal differentiation of these cells, as shown by MGG staining and FACS analysis, and completely abolished their clonogenic activity in methylcellulose ( Fig. 1, F and G). In contrast, acitretin or NRX only elicited terminal differentiation (Fig. 1 F) and treated methylcellulose cultures exhibited normal clonogenic growth ( Fig. 1 F). Thus, in RARA-transformed cells, transcriptional activation suffices to initiate differentiation, whereas RARA degradation underlies the loss of clonogenic activity, dramatically illustrating the pharmacological dissociation of these two previously intricately linked biological responses.

Uncoupled retinoids differentiate PML/RARA-transformed cells without affecting their clonogenic activity ex vivo
We similarly examined the effects of these retinoids on PML/ RARA-expressing cells. In rars / MEFs stably expressing PML-RARA, two primary target genes, rarb and tgm2, were similarly up-regulated by the three drugs ( Fig. 2 A). Yet, in these cells or in PML/RARA-transformed lin  murine hematopoietic progenitors, PML/RARA was completely degraded by RA treatment, but unaffected by treatment with acitretin or NRX, even at high concentrations ( Fig. 2 B and not depicted). In methylcellulose cultures, acitretin or NRX again induced terminal differentiation of transformed primary progenitors ( Fig. 2 C, top), but did not affect clonogenic growth, contrary RARA degradation. In contrast, antidepressive agents of different families promoted RARA degradation. Unexpectedly, RA-triggered EGFP-RARA degradation was antagonized by etretinate, a well-known retinoid used for treating psoriasis. This suggested that some retinoids may activate transcription without necessarily degrading RARA. Accordingly, contrary to RA, etretinate, acitretin, its active metabolite or NRX195183 (NRX), a well-characterized RARA-specific agonist ( Fig. 1 A), were all incapable of degrading RARA in immortalized rara,b,g / mouse embryonic fibroblasts (rars / MEFs) stably reexpressing RARA (Fig. 1 B) or in cells expressing endogenous RARA (not depicted). Yet, acitretin and NRX, at a concentration of 1 µM, activate the transcription of primary RARA target genes (rarb or cyp26a1) or synthetic reporters (RARE3-Tk-Luc) as RA (Fig. 1, C and D). Moreover, in keeping with previous studies (Beard et al., 2002;Louisse et al., 2011), RA, NRX, and acitretin had similar dose-responses (and kinetics) for the activation of RARE3-Tk-Luc or for transcriptional activation of endogenous cyp26a1 (Fig. 1 D and not depicted). We finally performed array analysis of RARAtransduced rars / MEFs treated with RA, acitretin, or NRX. At 4 or 8 h, 1 µM retinoid displayed similar activation patterns, although the mean magnitude of induction was reduced by 25% with acitretin or NRX when compared with RA ( Fig. 1 E), possibly reflecting the role of nuclear receptor degradation in sustained activation (Geng et al., 2012).
We then compared the biological effects of these three retinoids in RARA-transformed murine primary hematopoietic to RA, which completely abolished colony formation (Fig. 2 D and not depicted). In the presence of uncoupled retinoids, differentiated PML/RARA-transformed cells could even be serially replated more than three times (unpublished data). Complete differentiation was similarly triggered by all three agents in liquid cultures (Fig. 2 C, bottom).

In vivo comparison of retinoids highlights the key role of PML/RARA degradation in APL clearance
This unexpected dissociation between differentiation and clonogenic activity ex vivo prompted us to examine the effects of these drugs in vivo. For this, we used a well-characterized APL mouse model (Brown et al., 1997;Lallemand-Breitenbach et al., 1999), in which APL cells derived from PML/RARA transgenics are serially transplanted in syngeneic hosts. Mice were treated with retinoids when the disease was established, allowing analysis of tumor response (bone marrow cell differentiation, growth, clonogenic activity as tested by transplantation in secondary recipients) and of mouse survival. A 7-d RA treatment yielded a very significant prolongation of survival (median of 50 vs. 18 d). The survival benefit was considerably smaller with NRX, acitretin, or etretinate, the metabolic precursor of acitretin identified in the original screen, when compared with RA (P < 10 4 ; Fig. 3 A and not depicted). Yet, retinoid plasma concentrations were measured in the same range (Fig. 3 B), target gene activations at 6 h were highly comparable (Fig. 3 C) and differentiation, as assessed by morphology after 3 d of in vivo treatment, was similar ( Fig. 3 D, top). We could detect some differences in the expression of cell surface differentiation markers (Fig. 3 D,  bottom), likely reflecting reappearance of some nonleukemic cells in the bone marrow of RA-treated mice. As expected, in APL blasts invading the bone marrow, PML/RARA was completely degraded after 3 d of RA treatment, whereas its level was unaffected in animals treated with uncoupled retinoids (Fig. 3 E).
In line with our ex vivo observations, abundance of APL blasts (as assessed by the quantification of PML/RARA DNA in the bone marrow of APL mice) was significantly reduced after 3 (or 6) d of RA-treatment, but, again, was largely unaffected by the two uncoupled retinoids (Fig. 3 F). To directly assess leukemia-propagating activity in the marrows of treated (G) Survival of secondary recipients inoculated with 10 6 bone marrow cells from 3-or 6-d treated APL mice. 10 mice per group for NRX or etretinate, 20 for RA and controls at 3 d. 8 per group at 6 d. Pooled data from 3 independent experiments. Log rank test was used to evaluate significance of the survival difference between secondary recipients injected with bone marrow cells from RA-or uncoupled retinoid-treated APL mice.

Br ief Definitive Repor t
for retinoid pharmacology and underscoring the importance of determining the structural bases for these differences. In APL, our data formally demonstrate that RA-elicited transcriptional activation promotes cell differentiation, whereas only PML/RARA loss fully abolishes leukemia-initiating activity. Sustained levels of RARA or PML/RARA protein may be required for self-renewal, independent from the transcriptional status of direct target genes, although it remains possible that a small subset of targets is differentially regulated by the three retinoids. Our findings explain why many retinoids, like etretinate, that are not efficient RARA degraders promoted some ex vivo and even in vivo tumor differentiation in patients, but failed to clear the disease (Breitman et al., 1981;Flynn et al., 1983;Sampi et al., 1985), an endpoint only later achieved by all-trans-RA (Huang et al., 1988). They also explain why genetically RA-resistant APLs undergo terminal differentiation through rexinoid-mediated transcriptional activation by the PML/RARA-RXRA complex (Guillemin et al., 2002;Altucci et al., 2005), but fail to regress upon treatment because PML/RARA is not efficiently degraded. Many leukemia-associated fusions repress differentiation genes. These fusions have been targeted by several transcription-modulating agents, such as cytokines or histone deacetylase inhibitors (Johnstone, 2002;Altucci et al., 2005;Leiva et al., 2012). Our results suggest that even if those drugs achieve transcriptional reactivation and restore differentiation, they are unlikely to clear the disease. Finally, these observations unify the modes of action of RA and arsenic and shed light on the dramatic synergy between RA and arsenic in mice or patients (Lallemand-Breitenbach et al., 1999;Rego et al., 2000;Hu et al., 2009;de Thé and Chen, 2010). Oncoprotein degradation may thus represent a feasible and efficient therapeutic option in other malignancies (Ablain et al., 2011).

MATERIALS AND METHODS
Chemicals, cell culture, and viruses. PML/RARA and RARA transduction of lineage negative (Lin  ) primary hematopoietic progenitors was performed exactly as previously described (Du et al., 1999). ATRA, Acitretin animals, we transplanted 10 6 bone marrow cells in syngeneic secondary recipients and monitored their survival. In that setting, 3-d treatments with NRX or acitretin yielded modest effects (likely reflecting differentiation), whereas the survival of secondary mice was very significantly enhanced by RA-treatment, with 30% of injected animals never developing the disease (Fig. 3 G, left). An even more dramatic difference was noted using marrows of APL mice treated for 6 d (Fig. 3 G, right).

Differentiated blasts still expressing PML/RARA efficiently reinitiate APL
To rule out that leukemia-initiating activity of acitretin-or NRX-treated APL cells (Fig. 3 G) may reflect the presence of remaining undifferentiated blasts in the inoculates, we stringently sorted Mac-1 high /Gr-1 high cells from APL mice treated for 3 d with RA, NRX, or etretinate (Fig. 4 A). The quality of the sorting was verified by examination of cell morphology on MGG stains and by resorting (unpublished data). As expected, differentiated blasts from NRX-or etretinate-treated animals displayed abundant PML/RARA microspeckles, whereas those from RA-treated animals did not (Fig. 4 B). Strikingly, these cells reinitiated APLs when reinjected in irradiated syngeneic hosts, whereas those from RA-treated mice never did, even when 10 5 cells were reinjected (Fig. 4 C and not depicted). We cannot formally exclude the presence of very rare undifferentiated cells in the inoculum, despite their absence in 100-fold excess cells of RA-treated APL mice, which exhibit similar patterns and kinetics of differentiation. Interestingly, the resulting leukemia exhibited classical promyelocytic features, illustrating in vivo dedifferentiation of the terminally differentiated APL cells used for inoculation. Collectively, these data pharmacologically establish the uncoupling of differentiation and APL regression in vivo and underscore the key role of PML/RARA degradation in APL eradication.
Our findings have important implications for basic retinoid pharmacology because they demonstrate the existence of drugs that only activate (such as NRX or etretinate) or that activate and degrade (such as RA), revealing new perspectives