{'fraction_non_alphanumeric': 0.04522890237175951, 'fraction_numerical': 0.02978488692774407, 'mean_word_length': 5.212328767123288, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Immunophenotyping of low‐grade B‐cell lymphoma in blood and bone marrow: poor correlation between immunophenotype and cytological/histological classification

Results of immunophenotypic examinations of peripheral blood and/or bone marrow (BM), involved in low‐grade B‐cell non‐Hodgkin's lymphomas, were compared with the results of cytomorphological and histopathological examinations in 133 adult patients. 69 cases of chronic B‐lymphocytic leukaemia (B‐CLL), 16 centrocytic (CC) lymphomas, 14 centroblastic‐centrocytic (CB/CC) lymphomas, 15 immunocytomas (IC), 10 cases of hairy cell leukaemia (HCL), four prolymphocytic leukaemias (PLL), two B‐CLL in transformation, one splenic lymphoma with villous lymphocytes (SLVL), one hairy cell leukaemia variant (HCL‐V), and one lymphocytic lymphoma (LC) were classified according to the Kiel and /or FAB classification. Leukaemic disease was found in 105 cases. The following markers were used for immunocytology (APAAP technique) of blood and/or BM smears: CD 19, CD 5, CD 10, CD 11c, CD 14, CD 21, CD 22, CD 23, CD 25, CD 38 and TdT. All cases tested showed CD 19, but no TdT expression. Every case of HCL had a distinct phenotype with expression of CD 11c, CD 22 and CD 25 and the lack of CD 5 and CD 23 antigens. In all other NHL cases a very heterogenous expression of CD‐antigens with no significant correlations to the cytomorphological subtypes was found. The expression of CD 5 is a frequent but inconstant finding in lymphoproliferative diseases other than B‐CLL, so 50% of CB/CC, 75% of CC and 80% of IC were CD 5 positive. Our results indicate that, with the exception of HCL, the diagnostic relevance of immunophenotyping for the classification of cytomorphologically and histopathologically defined subtypes in blood and/or BM is of very limited value.