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Determination of sodium levothyroxine in bulk, tablet, and injection formulations by high-performance liquid chromatography.

Sodium levothyroxine was determined in bulk drugs, tablets, and injections by high-performance liquid chromatography (HPLC). Levothyroxine was separated from excipients and impurities on a 10-microns cyanoalkyl column using an acetonitrile-water-phosphoric acid mobile phase. The HPLC method is shown to be linear, accurate, and precise, and the results obtained by the HPLC and USP XX methods are compared.