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Optical image analysis of fat cells for indocyanine green mediated near-infrared laser treatment

Fat tissue engineering by near-infrared laser treatment is investigated in vitro. For the quantitative estimation of efficiency of treatment and consequential processes on the cellular level, a computational method for optical image analysis is proposed. The method is based on the fractal dimension data analysis of fat cell structure. The conducted experiments confirm the feasibility of the proposed method to obtain quantitative information of the cellular processes. The results indicate that the proposed method is promising for estimating spatial changes of biological structure.