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Properties of TAS‐1D3, a Tuberculin‐Active Substance from BCG, in Regard to Delayed Hypersensitivity

The properties of TAS‐1D3, a tuberculin‐active substance purified from the cell extract of Mycobacterium bovis BCG, were studied in vivo and in vitro. In the delayed hypersensitivity skin reaction, TAS‐1D3 showed far more potent activity than tuberculin purified protein derivative (PPD) in guinea pigs sensitized with BCG vaccine. This was consistently observed from 6 to 24 weeks after sensitization. The histological findings of the skin reaction to TAS‐1D3 were similar to those of the reaction to PPD. Moreover, TAS‐1D3 induced well both thymidine incorporation and the production of migration inhibitory factor (MIF) by the spleen cells from guinea pigs sensitized with BCG vaccine. In contrast, TAS‐1D3 showed weaker activity than PPD in guinea pigs sensitized with either heat‐killed M. tuberculosis Aoyama B or heat‐killed M. tuberculosis H37Ra, and it weakly stimulated the spleen cells from animals sensitized with M. tuberculosis Aoyama B to incorporate thymidine and to produce MIF.