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The Complete Mitochondrial Genomes of Six Heterodont Bivalves (Tellinoidea and Solenoidea): Variable Gene Arrangements and Phylogenetic Implications

Background Taxonomy and phylogeny of subclass Heterodonta including Tellinoidea are long-debated issues and a complete agreement has not been reached yet. Mitochondrial (mt) genomes have been proved to be a powerful tool in resolving phylogenetic relationship. However, to date, only ten complete mitochondrial genomes of Heterodonta, which is by far the most diverse major group of Bivalvia, have been determined. In this paper, we newly sequenced the complete mt genomes of six species belonging to Heterodonta in order to resolve some problematical relationships among this subclass. Principal Findings The complete mt genomes of six species vary in size from 16,352 bp to 18,182. Hairpin-like secondary structures are found in the largest non-coding regions of six freshly sequenced mt genomes, five of which contain tandem repeats. It is noteworthy that two species belonging to the same genus show different gene arrangements with three translocations. The phylogenetic analysis of Heterodonta indicates that Sinonovacula constricta, distant from the Solecurtidae belonging to Tellinoidea, is as a sister group with Solen grandis of family Solenidae. Besides, all five species of Tellinoidea cluster together, while Sanguinolaria diphos has closer relationship with Solecurtus divaricatus, Moerella iridescens and Semele scaba rather than with Sanguinolaria olivacea. Conclusions/Significance By comparative study of gene order rearrangements and phylogenetic relationships of the five species belonging to Tellinoidea, our results support that comparisons of mt gene order rearrangements, to some extent, are a useful tool for phylogenetic studies. Based on phylogenetic analyses of multiple protein-coding genes, we prefer classifying the genus Sinonovacula within the superfamily Solenoidea and not the superfamily Tellinoidea. Besides, both gene order and sequence data agree that Sanguinolaria (Psammobiidae) is not monophyletic. Nevertheless, more studies based on more mt genomes via combination of gene order and phylogenetic analysis are needed to further understand the phylogenetic relationships in subclass Heterodonta.


Introduction
Mitochondrial DNA (mtDNA) is the only extranuclear genome in animal cytoplasm [1]. Most metazoan mitochondrial genomes are covalently closed circular molecules which range from 14 to 42 kb in length [2], but see [3]. The typical mitochondrial genome contains the same 37 genes: 13 for protein subunits of oxidative phosphorylation enzymes (atp6, atp8, cox1-3, cob, nad1-6 and nad4l), two for mitochondrial ribosomal RNAs [small and large subunit ribosomal RNA (rrnS and rrnL)] and 22 for the transfer RNA genes (tRNAs) genes necessary for translating these 13 proteins [4]. In general, there are few intergenic nucleotides except for a single large non-coding region generally thought to contain elements that control the initiation of replication and transcription [4]. Owing to abundance of mitochondria in cells, lack of recombination, maternal inheritance (except for [5]), absence of introns, and higher evolutionary rates, mtDNA sequences are extensively used for comparative and evolutionary genomics, molecular evolution, population genetics, species identification, and phylogenetic relationships at various taxonomic levels [6][7][8][9].
For some phyla of animal, mitochondrial gene arrangements seem seldom to have changed. With few notable exceptions, those vertebrates studied, for instance, have identical gene arrangements [10]. However, mollusks, especially bivalves, which display an extraordinary amount of variation in gene arrangement, challenge this rule. Gene arrangement has been shown to be very powerful characters for reconstructing evolutionary relationships, and the rapidity of rearrangement within a lineage determines the level at which rearrangements are likely to be phylogenetically informative [10,11].
In recent studies, phylogenetic analysis based on complete mt sequence data have proved to enhance resolution and statistical confidence of inferred phylogenetic trees when compared with analyses based only on small portions of the mtDNA [12][13][14][15]. With technological and methodological advances, and associated decreasing costs of DNA sequencing, the amplification and sequencing of whole mt genomes has become routine [16]. Consequently, there have been significant increases in the number of complete mitochondrial sequences available during the last ten years. Nevertheless, to date, only ten complete mitochondrial genomes of Heterodonta, which is by far the most diverse major group of Bivalvia [17], have been determined.
Heterodonta, encompassing richly speciose families such as the Cardiidae, Tellinidae, Veneridae and Lucinidae, and including major economic groups such as clams, cockles, geoducks and razor shells, can be hugely abundant in both marine and freshwater systems, and of considerable ecological importance in community structure as well as a trophic resource [17][18][19]. However, taxonomy and phylogeny of Heterodonta are long-debated issues, and a complete agreement has not been reached yet, even if this subclass has a rich fossil history extending from the Lower Palaezoic, with major radiations in the Late Mesozoic [20][21][22]. In particular, the morphologically-inferred phylogenies of subclass Heterodonta were challenged by recent phylogenetic studies based on molecular data. For example, the Gastrochaenidae and Hiatellidae do not form a monophyletic group with the other families of the order Myoida [23][24]. Monophyly of the Lucinoidea is not supported, with the families Thyasiridae and Ungulinidae not closely related to the Lucinidae [25].
The superfamily Tellinoidea of the subclass Heterodonta consists of five families (Tellinidae, Donacidae, Psammobiidae, Semelidae, Solecurtidae) [26]. Based on the information of paleontology and morphology of Tellinoidea, large numbers of research works have been performed to study the evolutionary history and taxonomy within this superfamily over a long time [27][28][29][30]. Howbeit, compared with molecular analyses carried out to investigate relationships within individual families of Heterodonta (e.g., Veneridae [31,32], Sphaeriidae [33], Thyasiridae [34]), there have been few attempts to make comprehensive analysis of phylogenetic relationships of Tellinoidea on molecular level so far, not to mention analysis based on complete mitochondrial genome.
In this paper, we newly sequenced the complete mt genomes of six heterodont bivalves, including five from four families (Tellininae, Psammobiidae with two species, Semelidae, Solecurtidae) within superfamily Tellinoidea and one from superfamily Solenoidea which was ever classified in Tellinoidea, and compared their different gene arrangements. In addition, the six newly determined sequences, together with the heterodont mt genomes available in GenBank, were used to recover the phylogeny of Heterodonta in order to resolve some problematical relationships among this subclass.

Genome composition
The main structural features of the six newly sequenced mt genomes in this study are summarized in Table 1 . Length differences are mostly in virtue of the variation in tandem repeats within the non-coding region. The placement of all coding genes on the same strand and the lack of one protein coding gene atp8 are the most distinctive features of marine bivalve mt genomes [35,36], without exceptions for six studied species. The overall A+T content of six newly sequenced complete mt genomes ranges from 59.19% (S. scaba) to 67.08% (S. constricta). In addition, overlapping genes, which are presumably to help prevent rearrangements of gene order and loss of genes during evolution in mammalian [37], are a common phenomenon in all newly sequenced mt genomes (Table 1). Table 1 shows the initiation and termination codons for the 12 protein-coding genes (PCGs) encoded by the six mt genomes. Most of PCGs (64/72) appear to start with the conventional codon ATN (ATG, N = 34; ATA, N = 22; ATT, N = 8), which is typical for metazoan mt genomes [2]. There are also TTG (N = 3) and GTG (N = 5) acted as start codons, which are not unusual start codons in molluscan mt genomes but in several gastropod mt genomes [38]. All 12 PCGs of six mt genomes end in full termination codon (TAG, N = 37; TAA, N = 34), except for nad4 gene of S. diphos ending with the incomplete stop codon T which may be modified to a complete TAA stop codon via posttranscriptional polyadenylation [39]. In contrast to the available heterodont bivalves mt genomes from GenBank, the mt genome of S. olivacea has the longest cox1 (1758 bp) and nad2 (1077 bp) genes, S. scaba has the longest cox2 (1206 bp) and nad6 (633 bp) genes, and S. diphos has the shortest nad4 (1303 bp) gene.

Transfer and ribosomal RNA genes
In the mt genomes of metazoan, almost all amino acids but leucine and serine are decoded by only one tRNA each [40]. Without exception, there are 22 typical tRNAs interspersed throughout the mt genome of M. iridescens, S. divaricatus, and S. constricta. The trnF is missing in both S. diphos and S. olivacea, and S. scaba lacks trnY and trnS1. Deficiencies of tRNA genes are often observed in protozoans, fungi, algae, plants and low metazoans [41,42]. In this study, most of tRNAs can be folded into the typical secondary structures (not shown).
BLAST searches indicated approximate locations of the two rRNA genes, but their exact boundaries can not been determined [43]. The size of rrnL flanked by nad6 and atp6 in all six mt genomes ranges from 1228 bp (S. constricta) to 1380 bp (S. divaricatus). The length of rrnS varies from 861 bp (S. scaba) to 949 bp (S. olivacea). The rrnS genes of M. iridescens, S. diphos and S. divaricatus position in between trnG and major non-coding region. However, rrnS genes of S. olivacea, S. scaba, and S. constricta lie in between cox2 and trnS1, between trnW and cox2, and between trnM and cox3, respectively. Both lengths of rrnL and rrnS are within the range of genome sizes of already sequenced molluscan mtDNAs.

Non-coding regions
There are a large number of non-coding regions (NCR) including in the six mt genomes each. The number of NCR varies from 16 (S. diphos and S. olivacea) to 25 (S. constricta). The total length of unassignable nucleotides ranges from 1022 bp (6.25% of the genome) in S. diphos to 2730 bp (15.01% of the genome) in S. olivacea (Table 2).
Due to lacking discrete conserved sequence blocks, the control regions of invertebrates' mt genomes, unlike those of vertebrates, are not well characterized [44]. In general, the mt genome contains one major non-coding region with some peculiar patterns (e.g. AT-rich, hairpin structures, T-stretch, C-rich,tandem repeats), believed to play a role in initiating and/or regulating mitochondrial transcription and replication [2,[45][46][47][48]. The largest non-coding region (MNR) of the six mt genomes with all the patterns mentioned above is identified as a putative control region (CR). As highly rearranged gene order in bivalves, the MNR is not conserved at the same location among bivalves [42]. In this study, four different locations (between rrnS and trnM, trnG and trnI, cob and trnG, nad2 and trnK) of MNR occur.
Among the six mt genomes, the MNRs vary in size from 674 bp (S. diphos) to 2272 bp (S. olivacea) ( Table 2). Moreover, the A+T content of the putative CR in each mt genome is higher or slightly lower than that of the whole mt genome. There are some sections of nucleotide sequence existed in MNR, all of which can form a typical hairpin-like secondary structures ( Figure S1). The conserved flanking sequences around the hairpin structures exhibit conserved motifs: 59-flanking sequences show a TATA element (except for S. divaricatus) which has also been reported in Crustacea [49], while 39-flanking sequences possess a TTTAT element in M. iridescens, S. scaba, S. olivacea and S. divaricatus. It is assumed that these structures are of functional importance involving in the origin of the replication of mtDNA [48]. Long T-stretches of 18 bp, 15 bp, 18 bp and 13bp are observed in the MNR of S. diphos, S. olivacea, S. divaricatus and S. constricta, respectively, which may provide essential signals for the replication initiation of mtDNA [46]. In addition, the C-rich sequences, predicted to facilitate formation of the D-loop by decelerating the extension of heavy-strand synthesis at this location in some vertebrates [45], exist in the MNR of M. iridescens, S. scaba and S. olivacea.
Tandem repeats are also detected in MNR of five mt genomes ( Figure S2; Figure S3; Figure S4; Figure S5; Figure S6; Figure S7), but that of S. diphos. Especially in MNR of M. iridescens mt genome, three distinct tandem repeat units are found, one of which comprises 14.4 nearly identical copies of a 54 bp unit. Besides, S. olivacea has 2 copies of 98 bp and S. scaba has 2.8 copies of 109 bp. Such large tandem repeat units are also reported in the bivalves Acanthocardia tuberculata [50] and Placopecten magellanicus [51]. Further study on tandem repeats in the control region is needed, as it is important to illuminate the functional implications of the repeats and the molecular mechanisms that generate the repeats [52].

Gene order rearrangements
In contrast with other metazoans, the phylum Mollusca has long been known to exhibit an exceptionally variable arrangement of genes within mitochondrial DNA [53][54][55]. So far, due to coding on one strand probably, all bivalves whose mt genomes have been presented display enormous gene rearrangements (but see oysters [56]). It is suspected that coding on both strands may be inhibitory to mt genome rearrangement, because rearranging a genome with dual-strand coding may be more complicated and cause more harm than that codes on one strand [57].
In this study, we compare the gene order rearrangements of six newly sequenced mt genomes (Figure 1). The six heterodont bivalves exhibit five different gene orders, among which M. iridescens and S. divaricatus have the identical gene order. Furthermore, the five patterns of gene arrangement differ from any gene order ever reported in molluscs. The gene order of S. constricta is remarkably unlike that of five other species, even excluding the tRNA, which might indicate the relatively distant relationship as also revealed in the phylogenetic analysis in this study (see below). And five other complete mt genomes differ primarily in the position of tRNA genes, whose secondary structures allow them to translocate more frequently than either rRNAs or protein coding genes [58,59]. When disregarding tRNA genes, all five species belonging to superfamily Tellinoidea show the same gene arrangements except for translocations of genes rrnS and cox2 in S. scaba. The relatively high level of conservation in the gene order may verify the close lineage relationship. In addition, there are three small blocks, trnK-nad4l, trnV-trnW and nad6-rrnL, and three large blocks, cox1-nad4-trnH-trnS2-trnE-nad3, trnT-trnL1-trnD-trnL2-nad1-trnN-nad5-trnR-cob and atp6-cox3-nad2-trnP-trnQ-trnC-trnA, shared by S. olivacea and S. scaba.
One of our noteworthy finding during this study is that S. diphos and S. olivacea, which belong to the same genus Sanguinolaria, have different gene arrangements with three translocations of trnI, trnV-trnW-trnG and trnM. The case that differences in the gene arrangement occur in the same genus is seldom reported, yet in genus Dendropoma [16] and genus Crassostrea [57]. Besides, M. iridescens, S. divaricatus and S. diphos have a completely identical gene order, if lacking a trnF in S. diphos is ignored. As described above, it is surprising that the gene arrangement of S. diphos is more similar with that of M. iridescens and S. divaricatus than that of S. olivacea, while S. diphos and S. olivacea should have close relatives according to traditional taxonomy. Meanwhile, this result is consistent with the conclusion from the phylogenetic analysis (see below). Early analyses of mtDNA had led to the proposition that a comparative analysis of mt gene order could proved to be a useful phylogenetic tool [11,60,61]. By mt gene order comparisons, Smith et al. provided evidence that two echinoderm classes, sea stars and brittle stars, form a monophyletic group to the exclusion of two others, sea cucumber and sea urchins [60]. Akasaki et al. examined the relationships of subclass Coleoidea via comparing extensive mt gene arrangements, and concluded that order Octopoda might be the most ancestral among this subclass Coleoidea in accordance with the phylogenetic tree [61]. In this study, the results obtained here support that comparisons of mt gene order rearrangments, to some extent, are a useful tool for phylogenetic studies.

Phylogenetic analyses of Heterodonta
ML and Bayesian trees based on amino acid and nucleotide sequences of 12 concatenated protein-coding genes (except atp8 gene) were performed to reconstruct phylogenetic relationships within heterodont bivalves ( Figure 2). The tree topologies based on amino acid and nucleotide sequences were largely congruent and received high supports in most nodes.
Using mt genome data, the KH and SH tests were performed to evaluate the alternative morphology-based hypotheses and previous molecular studies. All 7 alternative topologies including 2 topologies presented in our study were summarized in Table S1. The best topology was phylogenetic analysis based on amino acid data shown in Figure 2A (KH and SH tests p = 1.000), while the second best one was phylogenetic analysis based on nucleotide sequences in Figure 2B (KH test p = 0.005 and SH test p = 0.210). All other topologies were significantly rejected (KH and SH P,0.001) which confirmed the phylogenetic analyses based on our data.
Our analysis shows the two species of family Lucinidae form a single clade as a sister group to other heterodont bivalves, indicating that Lucinidae is monophyletic, in accordance with previous viewpoint of Taylor [17]. Five species belonging to family Veneridae, including Paphia euglypta, Venerupis philippinarum, Meritrix meretrix, Meritrix petechialis and Meretrix lusoria cluster together, supporting the monophyly of family Veneridae [62,63]. In the phylogenetic trees based on the amino acid data, the rock-boring and nestling Hiatella arctica belonging to the order Myoida is as a sister group with Solenidae+Pharidae (superfamily Solenoidea) questioning the subdivision of the subclass Heterodonta into two orders Veneroidea and Myoida, which corroborates the finding of previous molecular analysis [17,64]. It should be noticed that A. tuberculata evidenced as a member of superfamily Cardioidea based on molecular analysis [17,50], is placed in a long branch as a sister group to Hiatelloidea+Solenoidea, which is robustly supported by BI but ML based on 12-protein amino acid data (Figure 2A). While in the phylogentic analyses based on nucleotide data, the position of this species is as sister group with the other heterodont bivalves except for Lucinidae ( Figure 2B). Both of results are incompatible with a sister relationship of Cardioidea to Tellinoidea, reported by Campbell [23], Steiner and Hammer [65], Dreyer et al. [66], and Taylor et al. [34] based on short fragments of nuclear gene or mt DNA. Therefore, more phylogenetic analyses based on more molecular data, in addition to morphological characters, are required in order to resolve the relationship among the subclass Heterodonta in the future. The position of genus Sinonovacula has been debated over a long time. Using morphological evidences such as the formation of the siphons and so on, Sinonovacula had previously been suggested by Yonge [67,68] that it should be removed from the family  Solenidae where it was placed by Ghosh [69] and transferred to the Tellinoidea. This decision was accepted and followed by Keen [70], Habe [71] and Vokes [72] who transferred Sinonovacula to Solecurtidae within the Tellinoidea. Subsequently, using shell and anatomical characters, von Cosel [73] retransferred Sinonovacula to Solenoidea. And then, the result of phylogenetic analyses of heterodont bivalves based on rRNA genes by Taylor [17] was in agreement with von Cosel's decision. Our phylogenetic analyses of multiple protein-coding genes not only show that S. constricta is distant from the Solecurtidae belonging to Tellinoidea, but also indicate that S. constricta is as a sister group with Solen grandis of family Solenidae. In other words, we prefer classifying the genus Sinonovacula within the superfamily Solenoidea and not the superfamily Tellinoidea.
All five species of superfamily Tellinoidea from four different families form a clade strongly supported by three trees (except for ML tree based on amino acid sequence), which corroborates the monophyly of Tellinoidea. This result is also reported by Taylor et al. [17]. However, according to the phylogenetic trees in this study, S. diphos has a closer relationship with S. divaricatus, M. iridescens and S. scaba rather than with S. olivacea, highly supported by BI and ML, implying that two species presently classified into Sanguinolaria (Psammobiidae) do not form monophyletic groups. These unexpected findings suggesting that the current taxonomy should be brought into question and a careful review of this genus is required. Similar conclusions that Semelidae, Donacidae and Tellinidae are not monophyletic were ever made when Taylor et al. analyzed familial relationships within Tellinoidea [17]. In fact, to date, there has no special research for the taxonomy of this superfamily based on molecular data. Thereby, in the future study, more detailed analyses with a larger taxon sampling and more rapidly evolving molecular markers including mt genome are still necessary in order to test the taxonomy of superfamily Tellinoidea based on morphology and clarify familial relationships within this superfamily.

Conclusions
In this study, we newly determined the complete mt genomes of six bivalves, increasing the number of complete mt genomes sequenced within subclass Heterodonta from 10 to 16. By comparative study of the gene order rearrangements and phylogenetic relationships of the species belonging to Tellinoidea, our results support that comparisons of mt gene order rearrangements, to some extent, are a useful tool for phylogenetic studies. Based on phylogenetic analyses of multiple protein-coding genes, we prefer classifying the genus Sinonovacula within the superfamily Solenoidea and not the superfamily Tellinoidea. Besides, both gene order and sequence data agree that Sanguinolaria (Psammobiidae) is not monophyletic. Nevertheless, more studies based on more mt genomes via combination of gene order and phylogenetic analysis are needed to further understand the phylogenetic relationships in subclass Heterodonta including superfamily Tellinoidea and Solenoidea.

Taxon sampling and DNA extraction
In this study, each of the six bivalve complete mt genomes sequenced was obtained from a single specimen. M. iridescens was collected from Leqing (Zhejiang province of China), S. diphos was from Beihai (Guangxi province of China), S. scaba was from Lingao (Hainan province of China), and meanwhile, all three were preserved in EtOH 95% in 2008. S. olivacea was sampled in Rizhao (Shandong province of China) and preserved frozen at 280uC in 2009. S. constricta and S. divaricatus were collected in Qingdao (Shandong province of China) and Rizhao in 2011, respectively.
The total genomic DNA was extracted from adductor muscle by a modification of standard phenol-chloroform procedure that has been described by Li et al. [74] and visualized on 1.0% agarose gel.

PCR amplification and sequencing
In order to design long-PCR primers, we first obtained partial cox1 and rrnL gene sequences, with the universal primers of LCO1490/HCO2198 [75], and 16SF/16SR [76], respectively. Polymerase chain reaction (PCR) was performed in a total volume of 50 ml including 2 U Taq DNA polymerase (Takara), about 100 ng template DNA, 1 mM forward and reverse primers, 200 mM of each dNTP, 16PCR buffer and 2 mM MgCl 2 . The PCR reaction was carried out in TaKaRa PCR Thermal Cycler Dice Model TP600 (Takara Bio Inc.) under the following conditions: an initial denaturation for 3 min at 94uC, then 35 cycles of denaturation for 45 s at 94uC, annealing for 45 s at 52uC, extension for 1 min at 72uC, and final extension for 5 min at 72uC.
Subsequently, each mitochondrial genome was amplified by long-PCR technique [77] based on the two specific primer pairs, which were designed from the obtained partial sequences using Primer Premier 5.0 (http://www.premierbiosoft.com/). PCR reactions were carried out in 50 ml reaction mixtures containing 33.5 ml of sterile distilled H 2 O, 5 ml of 106LA PCR buffer II (Mg 2+ plus, Takara), 8 ml of dNTP (10 mM each), 1 ml of each primer (10 mM), 0.5 ml of LA Taq polymerase (5 U/ml, Takara), and 1 ml of DNA template (50 ng). The long PCR cycling was set up with an initial denaturation step at 94uC for 2 min, followed by 35 cycles comprising denaturatin at 94uC for 20 s, annealing at 60uC for 35 s and extension at 68uC for from 7 to 15 min depending on the expected length of the PCR products. The process was completed with a final extension at 72uC for 10 min. PCR products were purified using EZ-10 spin column DNA gel extraction kit (Sangon Biotech), and then directly sequenced with the primer walking method. The sequencing was conducted on an ABI PRISM 3730 (Applied Biosystems) automatic sequencer.

Sequence analysis and gene annotation
All sequence data were analysed and arranged to create the full genome using the Seqman program from DNASTAR (http:// www.DNASTAR.com). Protein coding genes were analysed by ORF Finder [78] using the invertebrate mitochondrial code. The rRNA genes were identified with DOGMA [79] and BLAST searches [80]. The boundaries of each gene were determined with multiple alignments of other published bivalve mitochondrial sequences. The tRNA genes were identified by DOGMA and tRNAscan-SE Search Server [81] with a COVE score cutoff of 1.0 and the invertebrate mitochondrial genetic code for secondary structure prediction. The whole mt genome sequence was tested for potentially tandem repeats by TANDEM REPEAT FINDER, Version 4.0 [82].

Phylogenetic analyses
Eighteen molluscan mt genomes including six newly determined mt genomes, as well as those of all other heterodont bivalves, were used to illustrate the phylogenetic relationship of Heterodonta (Table 3). Chlamys farreri and Mimachlamys nobilis from the subclass Pteriomorphia served as outgroups. Owing to the fact that most bivalve species lack the atp8 gene, amino acid sequences of 12 concatenated protein-coding genes were used in phylogenetic analysis. The alignment of the amino acid sequences of each 12 mitochondrial PCGs was aligned with Clustal X [83] using default settings, followed by manual correction. After areas of dubious alignment were isolated using Gblocks [84] (default settings) and excluded from the analysis, the 12 separate amino acid sequence alignments were concatenated to a single multiple sequence alignment, which consisted of 2026 sites. The nucleotide sequence was substituted from the concatenated amino acid alignment and the final sequence consisted of 7076 sites.
Two methods: Maximum likelihood (ML) and Bayesian inference (BI) were used to reconstruct phylogenetic relationships of heterodont bivalves. For phylogenetic analyses based on amino acid data, MtArt+I+G evolutionary model was chosen as the bestfit model of amino acid evolution by ProtTest version 2.4 [85] at the default setting based on Akaike Information Criterion (AIC). As the MtArt evolutionary model is not available in MrBayes, the WAG model (the second best-fit model according to ProtTest) was used in Bayesian analysis. For phylogenetic analyses based on nucleotide data, the most appropriate model GTR+I+G was selected by MODELTEST [86] using the Akaike information criterion. The ML analysis was conducted with PHYML 3.0 program [87] and 1000 bootstraps were used to estimate the node reliability. BI was performed on combined database using MrBayes 3.1 [88] In the case of the Bayesian analysis, the Markov chain Monte Carlo (MCMC) were run for 5,000,000 generations (sampling every 100 generations) to allow adequate time for convergence. After omitting the first 25,000 ''burnin'' tree, the remaining 25,000 sampled trees were used to estimate the 50% of majority rule consensus tree and the Bayesian posterior probabilities. All phylogenetic parameters were checked with Tracer v 1.5 [89]. Alternative phylogenetic hypotheses from previous morphological and molecular studies were tested using the Kishino-Hasegawa (KH) test [90] and Shimodaira-Hasegawa (SH) test [91] implemented in CONSEL [92].  Table 3. List of the species whose mt genome sequences were used in phylogenetic analysis in present paper.