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Effect of P(Bs-co-Cl) on Protein Content and Enzyme Activity in Earthworm

The natural soil-contact method was used to investigate the toxicity of poly (butylene succinate-co-caprolactone) (P(BS-co-CL)) copolymers with different number-average molecular weight (Mn) on the protein content, cellulose enzyme activity and peroxidase activity in earthworm extraction. The results showed that: during the first two weeks, both cellulose enzyme activity and peroxidase activity of earthworms treated by polymer (Mn is 8.0×103), decreased with the increase of polymer content. In the polymer (Mn is 1.6×104) group, in the end (28 and 42 d), cellulose enzyme and peroxidase activity decreased with the increase of the polymer concentration. However, the polymer (Mn is 2.8×104) had no significant effect on the cellulose enzyme activity and peroxidase activity of earthworms. In addition, in the beginning (7 and 14 d) of the three treatments, the protein content increased with the raising of polymer concentration. Nevertheless, in the test treated by copolymers with high Mn, there was no significant changing.