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Characterization of fixed histological samples by means of digital holographic microscopy

In this paper we present our methodology for characterization of fixed histological samples using digital holographic microscopy. In contrast with typical workflows utilized for investigation of in vitro cultivated living cells, our approach doesn’t require segmentation of individual cells on phase images, which allows one to process phase images obtained from thick histological samples. The method was validated and demonstrated on several types of histological samples.