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[Multivariate twin crossover design parallel line assay for estimating potency of insulin].

Based on twin crossover design, a multivariate assay design was used in parallel line analysis to estimate the potency of insulin. This paper describes how to make the experimental design. After the analysis of variance according to assay, the potency and fiducial limits were calculated by the method (2.2). It has been applied by multivariate assay to estimate 80, 100 and 120% standard preparation and the recovery rate were between 94 and 108%. We also tested various batches and agent products. The average fiducial limits rates were less than 20%. In comparison with twin crossover analysis as usual, multivariate assay appeared to provide a more efficient utilization of experimental data, to increase weight of every animal for a more accurate estimation of potencies and to save on animal numbers 30-40%.