{'fraction_non_alphanumeric': 0.02327272727272727, 'fraction_numerical': 0.014545454545454545, 'mean_word_length': 5.521327014218009, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Demonstration of cholesterol esterified with polyunsaturated fatty acids in mycobacteria grown in vivo.

The presence of cholesterol in tubercle bacilli grown in vivo, but not in vitro, was first demonstrated by Youngner and Noll (1958). Asselineau (1966) explained that cholesterol of host origin would have been adsorbed on the bacillary surface. Kondo et al. (1970) confirmed the above observation with an additional finding that not a small amount of the bacillus-associated cholesterol were the esters with fatty acids. Unlike free cholesterol, such cholesterol esters were not removed efficiently from the bacilli by the extraction method of Fleischer, Fleischer and Stoechnius (1967), suggesting very close association of the latter lipid with the bacilli. This finding led us to an assumption that the ester type of cholesterol might be an indication of macrophage-mycobacteria interaction. Our recent study (1976) showed that cholesterol esters were accumulated in the incubation mixture of virulent mycobacteria and uninduced (resident) peritoneal macrophages of normal mice. This phenomenon was observed in the environment of Hanks' solution without added serum, and a suggestive evidence indicated that polyunsaturated long-chain fatty acids might be concerned with cholesterol esterification. As an extension of this observation, this paper is to demonstrate the