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Triazophos-induced vertical transmission of rice stripe virus is associated with host vitellogenin in the small brown planthopper Laodelphax striatellus.

BACKGROUND
Rice stripe virus (RSV) is one of the most serious rice diseases in East Asia. The small brown planthopper (SBPH), Laodelphax striatellus, is an economically important rice pest. SBPH transmits RSV horizontally and vertically, resulting in serious rice economic losses. Exposure to sublethal doses of insecticides can induce many physiological and cellular changes in insects. However, the mechanism underlying triazophos-induced RSV vertical transmission remains unclear. Here, we investigated the role of vitellogenin (Vg) in triazophos-induced RSV vertical transmission.


RESULTS
RSV vertical transmission rates were significantly induced by sublethal exposure to triazophos. The transcript abundance of viral RNA3 segment (RNA3) and capsid protein (CP) in the ovaries of SBPH were also significantly increased. Triazophos induced the expression level of LsVg and increased the contents of ovarian protein and fat body protein. Knockdown of Vg significantly reduced the expression levels of LsVg and LsVgR, and decreased RSV accumulations in the ovaries. dsVg-mediated downregulation could be rescued by exposure to triazophos. Vertical transmission rate of dsVg-injected group was significantly decreased compared with the dsGFP-injected group, and triazophos significantly rescued RSV vertical transmission rate of dsVg-injected group.


CONCLUSION
Our results indicated that triazophos-induced RSV vertical transmission was associated with Vg. This work will facilitate us to further elucidate sublethal doses of insecticides-mediated effects and develop new strategies for pest control. This article is protected by copyright. All rights reserved.