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Influence of initial medium content (pH, serum and Ca++ concentration) on leukocyte migration test.

The aim of the study was to check to what extent inhibition of leukocyte migration in capillary tubes depends on alteration in pH, serum content and Ca++ concentration in medium in which the test is started. We found that relatively wide variations of pH (between 6.4 and 8.2) did not influence the final result. The best conditions for leukocyte migration were created by addition of 5% calf foetal serum, whereas a contamination of medium with autologous serum made a proper interpretation of the test difficult because of a resulting dispersion of cells. Neither removal nor addition of Ca++ ions had a significant effect on migration of leukocytes, unless combined with a simultaneous presence of calcium ionophore A 23187 in 0.5 microM concentration.