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INVESTIGATION OF ADSORPTION EFFECTIVENESS OF KOKUM (GARCINIA INDICA) RIND RESIDUE ON SYNTHETIC DYES AND DETERMINATION OF BEST EXTRACTION PROCEDURE FOR KOKUM PHENOLS

The aim of the study is to investigate the potential use of kokum fruit for production of natural dye and scrutinizing the adsorption effectiveness of the residual with respect to time, dye concentration, adsorbent dosage and pH. Extraction of dried fruit rind of kokum was carried out by using various enzymatic methods and other modern techniques such as Microwave assisted extraction and Ultrasonication. Total phenolic content in fruit extract of Garcinia Indica was found to be 0.599GAE (Enzyme assisted) > 0.582GAE (Ultrasonication) > 0.461GAE (Microwave assisted) respectively in terms of Gallic acid equivalent (GAE). Adsorption studies were further made on the residual fruit rind under various operating parameters. Experimental data indicated that the adsorption capacity was dependent on operating variables such as contact time, p H , adsorbent dosage and dye concentration. The contact time required to attain equilibrium was around 120min.The adsorption data were correlated with the Freundlich and Langmuir adsorption isothermal models and were found satisfactory. All extracts were concentrated via Vaccum Evaporation and made free of solvents which have been used for extraction and the final concentrate obtained was used to dye cloth. Positive antimicrobial activity of kokum extract was documented for three strains viz. E.coli, Bacillus subtilis and Staphylococcus aureus.