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Complexation between Amylodextrin Oligomers and Selected Pharmaceuticals Measured through Capillary Electrophoresis.

The amylodextrin oligomers, tagged fluorescently at their reducing ends for laser fluorescence detection, were used as model solutes to investigate certain carbohydrate-drug interactions through capillary electrophoresis. The separation patterns of some oligomers were found to be altered significantly by the presence of selected pharmaceuticals used as buffer additives. The selectivity of complexation at a particular size of an oligomer could be judged from alterations in migration times and peak shapes. The formation of complex was influenced substantially by solutes' chemical environment (pH, ionic strength, and the nature and concentration of organic additives). The chemical nature of the guest molecules also played an important role in complexation. Using amylodextrins as chiral selectors, enantiomeric resolution of several pharmaceuticals was achieved electrophoretically. The conclusions drawn from electrophoretic data were found to be in close agreement with the results of a (13)C NMR study.