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Cancer cell culture—Methods and protocols

Since the publication of the first human cancer cell line (Hela cells) about five decades ago, cell culture has become a widely used technology. Our present understanding of the cell and molecular biology of cancers has been derived mainly from the use of cultured cancer cells. Thousands of ceil lines representing a wide spectrum of human cancers have been developed so far. The book, Cancer Cell Culture--Methods and Protocols, introduces the basic concept of cancer cell culture, as well as general cell culture technology, and describes many easy to follow methods for researchers seeking to use cell culture techniques in their laboratories. The book also describes in detail how to characterize and authenticate widely used cell lines by deoxyribonucleic acid fingerprint and cytogenetic methods, how to isolate and develop cell lines from cancers from different tissue origins, and how to eoculture different cell types. In addition, Cancer Cell Culture--Methods and Protocols also introduces how to prevent and recover cell lines from contamination, including bacteria and yeast, and how to detect and eliminate mycoplasma infection. A wide range of procedures encompassing many of the key functional features of cancer cells are also described in the book, including assays to evaluate elonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters, and methods to modify cancer cells, including protocols for transfection, development of drug resistance, immortalization, and establishing xenografts using Matrigels. Overall, Cancer Cell Culture Methods and Protocols is a good and user-friendly resource. Laboratories conducting cancer cell culture or regular tissue cultures will find this book a useful complement to their library and a handy reference book for routing cell culture protocols. Beginners in cell cultures will find this book easy to follow and a source for many needed basic cell culture techniques.