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Tm3+ -Sensitized NIR-II Fluorescent Nanocrystals for In Vivo Information Storage and Decoding.

In vivo fluorescence imaging in the second near-infrared window (NIR-II) affords deep-tissue penetration and high spatial resolution. Herein, we present a new type of Tm3+ -sensitized lanthanide nanocrystals with both excitation (1208 nm) and emission (1525 nm) located in the NIR-II window for in vivo optical information storage and decoding. Taking advantage of the tunable fluorescence lifetimes, the optical multiplexed encoding capacity is enhanced accordingly. Micro-devices with QR codes featuring the NIR-II fluorescence-lifetime multiplexed encoding were implanted into mice and were successfully decoded through time-gated fluorescence imaging technology.