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The origin and nature of organic nitrogen in soil as assessed by acidic and alkaline hydrolysis

The origin and nature of much of the organic nitrogen in soil is unknown or speculative. Alkaline hydrolysis was used to fractionate soils into alkali‐soluble and insoluble fractions. Alkali‐insoluble residues from a wide variety of soils contained largely fixed ammonium or chitin or both. Acid hydrolysis of alkali‐insoluble residues from soils, microbes, insects and well‐rotted plant litter confirmed the presence of hexosamine‐N.