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HSF4 regulates lens fiber cell differentiation by activating p53 and its downstream regulators

Cataract refers to opacities of the lens that impede the passage of light. Mutations in heat shock transcription factor 4 (HSF4) have been associated with cataract; however, the mechanisms regarding how mutations in HSF4 cause cataract are still obscure. In this study, we generated an hsf4 knockout zebrafish model using TALEN technology. The mutant zebrafish developed an early-onset cataract with multiple developmental defects in lens. The epithelial cells of the lens were overproliferated, resulting in the overabundance of lens fiber cells in hsf4null zebrafish lens. Consequently, the arrangement of the lens fiber cells became more disordered and irregular with age. More importantly, the terminal differentiation of the lens fiber cell was interrupted as the organelles cannot be cleaved in due time. In the cultured human lens epithelial cells, HSF4 could stabilize and retain p53 in the nucleus to activate its target genes such as fas cell surface death receptor (Fas) and Bcl-2-associated X apoptosis regulator (Bax). In the hsf4null fish, both p53 and activated-caspase3 were significantly decreased. Combined with the finding that the denucleation defect could be partially rescued through microinjection of p53, fas and bax mRNA into the mutant embryos, we directly proved that HSF4 promotes lens fiber cell differentiation by activating p53 and its downstream regulators. The data we presented suggest that apoptosis-related genes are involved in the lens fiber cell differentiation. Our finding that HSF4 functions in the upstream to activate these genes highlighted the new regulatory modes of HSF4 in the terminal differentiation of lens fiber cell.

Cataract is a major cause of adult blindness and congenital cataract is a major cause of childhood blindness. 1 At least 44 genetic loci and over 40 genes have been linked to congenital or early-onset cataract. [2][3][4] Heat shock transcription factor 4 (HSF4) has been associated with isolated cataract 5 and it belongs to the heat shock transcription factor family (including HSF1, HSF2, HSF3 and HSF4). These HSFs can respond to various stress stimuli and protect cells against proteotoxic damage. 6,7 More importantly, HSFs are also involved in regulating differentiation and development. 8 HSF4 differs from other HSFs in that it lacks C-terminal HR-C domain, which is responsible for the negative regulation of trimerization. 7,9 Moreover, whereas HSF1 and HSF2 are expressed in most tissues, HSF4 is predominantly expressed in the lens. 10,11 To investigate the function of HSF4, three Hsf4 knockout mouse models have been constructed. [12][13][14] All these three models developed early postpartum cataract. Histological analysis revealed that the nucleus and some cellular organelles of the secondary fiber cell persisted and the number of the epithelial cell increased. Associated with the altered biological processes, expression levels of the Hsf4 target genes were significantly changed. These genes include the ones encoding heat shock proteins (Hsp70, Hsp60 and Hsp27), diverse types of γ-crystallins, various forms of fibroblast growth factors or the cognate receptor (FGF1, 2, 4, 7 and FGFR1), the beaded filament proteins (Bfsp1/2) [12][13][14] and several other target genes (SKAP2, Vimentin). 15,16 Besides the knockout models, the HSF4 p.Arg116His mutation and the Hsf4(exon1)-DBD-EGFP hybrid gene transgenic mouse models have also been constructed. Both models developed postnatal lamellar cataract, which is similar to the phenotype caused by the mutations in the DBD of human HSF4 gene. 17,18 These transgenic mice provide excellent models to study the lamellar cataract. Together, these studies revealed important functional aspects of the HSF4 gene. However, the exact mechanisms by which Hsf4 regulates lens development and whose mutations cause cataract still remain largely unknown.
Lens differentiation is a process through which an epithelial cell containing a full spectrum of cellular organelles is converted into a fiber cell characterized by the accumulation of high concentrations of lens-specific proteins and the loss of essential organelles. 19 Recent studies demonstrated that lens differentiation is regulated by the same set of regulators responsible for the control of apoptosis. 19,20 These regulators include the tumor suppressor p53, [21][22][23][24][25][26][27][28] Bcl-2 family members, 25,[29][30][31][32][33] caspase family members, 19, small heat shock proteins 32,[41][42][43][44][45] and tumor necrosis factors. 46 Tumor suppressor p53 has been implicated in regulating lens development. During mouse lens development, the expression of p53/Mdm2 was spatiotemporally regulated. 24 Loss of p53 activity through expression of viral genes or the endogenous gene knockout induces posterior subcapsular cataracts. 21,47,48 Besides, overexpressing human p53 in mouse lens led to microphthalmia. 22 At the molecular level, p53 has been shown to regulate both major lens transcription factors c-Maf, Prox-1 26 and differentiation-related crystalline genes. 49,50 In addition, p53 regulates numerous apoptotic genes, some of which are implicated in regulating lens differentiation. For example, Fas and Bax mediate both extrinsic and intrinsic death pathways, which are merged to activate the downstream executional caspase3. It has been discovered that caspase3 is a key regulator of lens development. [29][30][31][32][33][34]36,38,39,51 The caspase3 −/− mice developed cataract at the anterior lens pole. 51 These findings indicated that p53 can regulate different sets of genes to control proliferation, apoptosis and differentiation of lens epithelial cells.
Our previous research discovered that HSF4 stabilizes p53 by inhibiting its ubiquitination and degradation. Through stabilizing p53, HSF4 can promote cell cycle arrest at the G1/S phase, thus protecting cells from overproliferation. 52 In this study, we generated an hsf4 knockout zebrafish line. The hsf4 knockout zebrafish developed early-onset cataract with multiple cataractogenic defects, which were caused by uncontrolled cell proliferation and differentiation. More importantly, we demonstrate here that HSF4-oriented p53 is necessary and essential in regulating these activities. In absence of HFS4, p53 activity was downregulated and the expression of its downstream genes including Fas and Bax was significantly attenuated. As a result, both external and intrinsic apoptotic pathways were attenuated; thus, the (b) Bsr1 assay. The upper picture was the genotyping results. PCR products from F2 zebrafish and wild type were subjected to the Bsr1 digestion. The wild-type allele cannot be digested, thus, only had one 466-bp band. The del7 allele could be digested by Bsr1, producing two bands that were 305 and 161 bp, respectively. Lanes (from left to right): three samples, WT control, negative control and size standard. The lower sequence showed the new Bsr1 restriction site generated by the 7-bp deletion in the mutant fish. (c) Sanger sequencing of WT and hsf4 mutant fish (del7, c.211-217del). The 7-bp deletion was pointed out with a box. (d) Western blot analysis of the hsf4 protein in the WT and hsf4 null zebrafish. The zebrafish hsf4 protein expressed in eukaryotic cells was used as a positive control. Tubulin was a loading control and the ov-hsf4 sample from the HLECs was the positive control. The anti-hsf4 antibody could recognize the translated product of the transcript XM_009293553(424aa). The black arrow pointed to the hsf4 band conveyed caspase3 activity was significantly decreased, leading to incomplete organelle degradation. Thus, our results illustrate a fundamental mechanism regarding how HSF4 controls normal lens development and prevents cataractogenesis.

Results
Establishment of the hsf4 knockout zebrafish using TALEN technology. A pair of TALENs targeting exon1 of hsf4 (XM_009293553) was designed on the website https:// tale-nt.cac.cornell.edu/ to knockout hsf4. 53 The selected target sites located in the DNA-binding domain are highly conserved between human and zebrafish 54 (Figure 1a). Then, we used Fast TALE Assembly kit to construct hsf4-TALENs. TALEN mRNAs transcribed in vitro were microinjected into zebrafish embryos at the one-to two-cell stage. Positive embryos validated by sequencing were raised to adult and named F0 zebrafish. Their offsprings (F1) were screened by T7e1 enzyme and were sequenced to confirm the mutations. We identified a truncation mutation (c.211_217del, p.Lys24-Glyfs10), named del7, which formed a new Bsr1 restriction site ( Figure 1b). Subsequently, we crossed F1 to obtain homozygotes (F2). Genotypes of F2 were validated by Bsr1 cleavage (Figure 1b) and sequencing (Figure 1c). And then, western blot detection was performed to ensure that our hsf4 knockout was effective. The result confirmed that no hsf4 protein existed in del7-mutant homozygotes ( Figure 1d). Thus, the homozygous hsf4 mutant zebrafish we acquired in this study are authentic hsf4 null zebrafish.
hsf4 knockout in zebrafish causes early-onset cataract. A slit lamp examination of the hsf4 null zebrafish eye revealed clear presence of cataract formation at different stages including 2M, 3M and 12M (Figure 2a). By 12M, the hsf4 null zebrafish lens became completely opaque ( Figure 2a). In contrast, the WT lenses were transparent at all ages examined ( Figure 2a). Defects in degenerating organelles are major causes of cataract. Thus, by observing denucleation status we can detect the onset of this defect. Using semiquantitative reverse transcription and polymerase chain reaction, we first determined that hsf4 was highly expressed in eyes at 48 hpf (Supplementary Figure 1). Shortly after that, denucleation began at 50 hpf, indicating that hsf4 was critically important in this process. Consistent with the expression pattern, no significant difference was observed between WT and hsf4 null zebrafish before 48 hpf. After 2 dpf, great differences have been discovered. The WT zebrafish completed denucleation of the primary fiber cells before 3 dpf, and the secondary fiber cells successfully disintegrated their nuclei. In contrast, in hsf4 null zebrafish lens, almost all the primary fiber cells in the lens core contained nuclei at 3 dpf. By 5 dpf, there were some nuclei still remaining in the lens core. The denucleation of the primary fiber cells eventually completed at 7 dpf, which indicated that the denucleation of the primary fiber cells was delayed but not abrogated when hsf4 was deleted (Figure 2b).
The denucleation of the secondary fiber cells seemed more severely affected within hsf4 null zebrafish. By the ages of 2 and 6M, we discovered that most of the fiber cells in hsf4 null lens contained intact nuclei, which were deposited together and even reached the most inner part of the lens. In the WT lens, however, the denucleation of the fiber cells was normal for we could only detect nuclei in the superficial differentiating fiber cells (Figure 2e). To our surprise, we found some spherical nuclei in the secondary fiber cells (Figures 2c-e). When the lens fiber cell differentiation initiates, nuclei start to elongate and become ovoid. Studies in bovine and chicken revealed that the volume and shape of the nuclei changed during disintegration. 55,56 Normally, the breakdown of the nucleus is a very rapid process; thus, the degenerating spherical nuclei are seldom found. The spherical nuclei we detected in the hsf4 null lens maybe the fiber cells that were about to degrade. As the hsf4 null lens contained an overwhelming majority of secondary fiber cells with ovoid nuclei, we reasoned that only a small proportion of the secondary fiber cells could disintegrate their nuclei in an hsf4-independent manner. Without hsf4, the denucleation of the secondary fiber cells appeared to come to a standstill.
hsf4 loss interrupts terminal differentiation of Zebrafish lens fiber cells. Previous research discovered that nuclei were preserved in the differentiating fiber cells as confirmed in hsf4 null zebrafish; it is not clear whether the cellular nonnucleus organelles are also preserved in hsf4 knockouts. Thus, we performed transmission electron microscopy of 5-M-old hsf4 null and WT zebrafish. As shown in Figure 3Af, the WT lens fiber cells were transparent and organelle-free, i.e., no dark stained structures interfering with the focusing of inside structure. In contrast, the fiber cells of the hsf4 null zebrafish contained intact organelles, mitochondria, endosome and lysosome besides nuclei, suggesting that organelle degradation was also interrupted without hsf4 ( Figure  3Aa-e). In the inner part of the lens, we could also detect intact nucleus ( Figure 3Ac) and also observed that the connection between cortical lens fiber cells was loose ( Figure  3Aa and b. In addition, the mutant fiber cells contained clustered vesicular structures (Figure 3Ad and e). These structures were early endosome, late endosome (LE) and LE-lysosome fusion body as observed from enlarged figures ( Figure 3c). Usually, LE will be degraded when it is translocated to and fused with lysosome at the perinuclear region. In the hsf4 null zebrafish lens, however, the deposited endosomes were mislocalized and left undegraded. Together, our study clearly revealed that the non-nucleus organelle degradation was also interrupted in the hsf4 null lens fiber cells.
hsf4 loss interrupts balanced cell proliferation and differentiation, which leads to pathological lens fiber architecture in Zebrafish lens. To testify whether the number of the lens epithelium cell was also increased in hsf4 null zebrafish, we performed paraffin section and DAPI staining on 5-M-old zebrafish. Under microscopy observation, we confirmed that the epithelial cells were overproliferated. In adult lens, the proliferation activity at the central region of the lens epithelium is extremely low; thus, overproliferation was more obvious and significant in the germinal zone (Supplementary Figure 2).
We performed frozen section and phalloidin staining to determine whether the overproliferated cells at the germinal zone would produce excessive number of lens fiber cells. Under microscopy observation, we found that the fiber cell was labeled with phalloidin immunofluorescence, forming a hexagonal fluorescence circle. However, these circles stack together to generate a regular structure (Supplementary Figure 4). The WT lenses from every time point we checked displayed the regular arrangement pattern. In contrast, notable differences showed in hsf4 null lenses. Compared with the WT lens, the fluorescence signals in hsf4 null lens were denser but well organized at 2M, suggesting that more fiber cells existed in hsf4 null lens (Figure 4a). At 6M, the standard arrangement pattern was severely interrupted (Figure 4b). By the age of 8M, the signals  (Figure 4c). In addition, we could also find some nuclei mislocalized in the anterior region of the 6 and 8M hsf4 null lenses. The above findings indicated that the arrangement of the fiber cells was disturbed (Figures 4b and c). Together, these results suggest that the overproliferation of the lens epithelial cells contributes to the excessive accumulation of fiber cells in the hsf4 null lens with age, which causes chaotic arrangement of the fiber cells in the limited space, leading to cataractogenesis.
HSF4-governed p53 stability and nuclear localization are essential to activate the Fas-mediated apoptotic signaling pathway. Continued with our previous research, we further investigated the roles of HSF4 and p53 in fiber cell differentiation. Firstly, we confirmed that HSF4 could stabilize p53 in human lens epithelial cell lines (HLECs). As expected, cells expressing GFP-HSF4 showed a stark enrichment of endogenous p53 in the nuclei (Figure 5a). Moreover, the endogenous p53 was predominantly localized in nuclei in a diffused pattern in cells transfected with GFP vector. In contrast, p53 was more focused and oriented in the nuclei but not in the cytoplasm of the cells expressing GFP-HSF4 fusion protein (Figure 5a). Western blot analysis of p53 levels in the separated nuclear and cytoplasmic fractions confirmed our immunofluorescence data (Figure 5b). On the other hand, p53 was obviously decreased in the nucleus when HSF4 was As previous studies have revealed that apoptotic regulators are implicated in regulation of lens differentiation, we tested Fas and Bax, two target genes of p53 that encode proteins mediating extrinsic and intrinsic apoptotic pathways. First, through real-time PCR analysis, we found out that both Fas and Bax were significantly upregulated when HSF4 was overexpressed in HLECs ( Figure 6a). In contrast, if HSF4 was silenced both Fas and Bax mRNA were significantly decreased (Figure 6b). Western blot results confirmed the changes of Fas and Bax at the protein level parallel with them at the mRNA level (Figures 6d-g). In addition, we noticed that the executor caspase3 was significantly activated in the cells expressing GFP-HSF4 compared with GFP control (Figure 6d). Consistent with this observation, the cleaved-caspase3 was decreased when we silenced HSF4 in HLECs (Figure 5f). Second, we determined whether HSF4-mediated upregulation of Fas and Bax was p53-dependent. To do so, the p53-null H1299 cells were transiently transfected with GFP vector or GFP-HSF4 fusion protein expression constructs. After 48 h, these cells were harvested for real-time PCR and western blot analysis. The results showed that both Fas and Bax were not altered at either mRNA or protein level (Figures 6c and h). Increased activation of caspase3 could not be detected in H1299 cells expressing GFP-HSF4 (Figure 6h). Thus, our results demonstrated that the activation of the Fas-mediated apoptotic signaling pathway by HSF4 was p53-dependent.
hsf4 loss can be partially rescued by overexpression of hsf4, p53 and fas. As HSF4 can regulate p53 stability and control Fas and Bax in a p53-dependent manner, we next sought to determine the functions of HSF4 regulation of p53 and its downstream target genes in governing lens development. To do so, we extracted proteins from hsf4 null and WT  (Figures 7a  and b). As p53 was downregulated, the activation of caspase3 was also significantly decreased, whereas the protein level of procaspase3 was not affected at two different time points we tested (Figures 7a and b). Together, these results demonstrated that HSF4 can induce caspase activation by stabilizing p53 in vivo.
To further test the functions of HSF4-controlled p53 and its target gene in regulating lens differentiation, we overexpressed these genes to determine whether they can rescue the denucleation defect in hsf4 null lens. To do so, we collected hsf4 null embryos and performed microinjection using hsf4, p53 and fas mRNA. As we have shown that the hsf4 null zebrafish displayed obvious defects in degrading the nuclei of the lens fiber cells after 3 dpf, we collected the injected embryos at 3 and 5d. Through frozen section and DAPI staining, we found that hsf4, p53 and fas could partially rescue the denucleation defect in hsf4 null lens (Figure 7c). These results demonstrated that hsf4 can regulate lens fiber cell differentiation by regulating p53 and its downstream apoptotic regulators.

Discussion
By constructing and analyzing the hsf4 null zebrafish, we demonstrated the following: (1) hsf4 null zebrafish develops an early-onset cataract and the pathologic condition remained with age; (2) the overproliferation of the lens epithelial cells contributes to excessive accumulation of the lens fiber cells, which interrupt lens normal arrangement pattern and lead to cataractogenesis; (3) at the cellular level, the denucleation of the primary fiber cell is much delayed in hsf4 null lens, and this becomes even more severe in the differentiation progress of secondary fiber cells. Both nuclei and other cellular organelles including mitochondria, endosome and lysosome cannot be disintegrated. This is another reason for cataractogenesis; (4) at the molecular level, by stabilizing p53, HSF4 can regulate both extrinsic and intrinsic apoptotic pathways to mediate its control of lens differentiation. Loss of HSF4 leads to downregulation of p53, inactivation of the p53-dependent death with clear attenuation of caspase3 activation and eventual halt of the lens differentiation. Together, our results reveal a fundamental mechanism by which HSF4 controls normal lens development and prevents cataractogenesis.
HSF4 is a major factor governing lens differentiation. The hsf4 null zebrafish developed an early-onset cataract with multiple defects as we summarized above. However, while the studies from the laboratories of both Nakai 12 and Mivechi 13 reported clear microphthalmia, we did not notice any obvious changes in eye size. Our results are consistent with the Hsf4 null mouse model reported by Hu 14 and the transgenic mouse models. 17,18 We noticed that the two models with microphthalmia were generated from C57Bl/6 mice, whereas the mouse models with normal eye size were Figure 5 HSF4 stabilizes and orients p53 in the nucleus to initiate. (a) p53 is retained in the nucleus by HSF4. HLECs were transfected with GFP-tagged HSF4 (green) plasmids and negative control GFP vectors, respectively. Cells were harvested 48 h after transduction followed by immunofluorescence stains with anti-p53 antibody. Arrows indicated the overlap of the p53 signals (red) and the HSF4 signals (green). Scale bar: 10 μm. (b) The nuclear distribution of p53 was increased when HSF4 was overexpressed in HLECs. (c) Nuclear-oriented p53 was decreased when HSF4 was silenced in HLE cells. The cytoplasmic and nuclear proteins were separated and subjected to western blot detection. Tubulin and FBL were used as specific markers for the cytoplasm and nuclear components, respectively HSF4 activates p53 to promote lens differentiation M Gao et al generated from S129 mice. We speculate that the difference of the genetic background causes inconsistency in the observed eye size. Of course, other possibilities could not be ruled out.
It is noteworthy that besides the nuclei and mitochondria, we found other membrane-bound organelles, including endosomes and lysosomes, persisting in hsf4 null lens. Under normal conditions, the matured LEs translocate to and fuse with lysosome at perinucleus regions to proceed to the unidirectional degradative pathway. Without hsf4, the LEs and lysosomes were mislocated. When the distribution of endosome and lysosome disperses throughout the cytoplasm, the degradation activity will be affected. Our previous studies have shown that HSF4 can upregulate lysosome activity in HLECs. 57 Furthermore, the p53 and Bax could increase the lysosome membrane permeabilization to trigger cell death. Thus, the accumulation of matured LE in the hsf4 null lens might be caused by the decreased activity of lysosome. However, HSF4 activates p53 to promote lens differentiation M Gao et al the exact mechanisms of why the distribution of the endosomes becomes dispersal and how hsf4 affects this process remain to be further investigated.
HSF4 stabilizes its key function mediator p53 to regulate lens differentiation at different levels. The balanced p53 activity in the ocular lens is essential for normal lens development. In ocular lens, HSF4 is one of the key factors to stabilize p53. It does so in different ways. First, HSF4 can stabilize p53 through the direct interaction with each other. 52 Secondly, HSF4 relies on its target gene products αBcrystallin and vimentin to stabilize p53. 16 αB-crystallin can interact with p53 to stabilize the later. 58 The HSF4-regulated upregulation of p53 can initiate cell apoptosis signaling, leading to the apoptotic cleavage of vimentin. The cleavage of vimentin leads to the release of cytosolic p53 for nuclear translocation, thus stabilizing p53 and amplifying cell death signal. 59 Finally, HSF4 can stabilize p53 through p53 target  (c) Overexpression of hsf4, p53 and fas could partially rescue the denucleation defect in hsf4null zebrafish lens. The hsf4, p53 and fas mRNA was microinjected into the hsf4 null zebrafish embryos. The denucleation status was checked at 3 and 5d, respectively, through the DAPI staining. Obviously, decreased numbers of nuclei were presented in the lens injected with hsf4, p53 and fas mRNA at both 3 and 5d. Scale bar, 20 μm HSF4 activates p53 to promote lens differentiation M Gao et al genes. The HSF4 target, αB-crystallin gene, is also regulated by p53 and αB-crystallin can interact with p53 to stabilize the later. 58 HSF4 regulates p53-mediated extrinsic and intrinsic death pathways to control lens differentiation. It has been well documented that the same set of regulators can be used for control of both apoptosis and lens differentiation. 19,20 In this regard, the HSF4-stabilized p53 has an important role. First, p53 can regulate major lens transcription factors such as C-Maf and Prox-1 to promote lens differentiation. 26 Second, it directly regulates the differentiation-related genes including members of the α-, βand γ-crystallin genes. 49,50,58 The promoted expression of βand γ-crystallin genes are symbols of lens differentiation, thus helping cell maturation in lens fiber. In addition, p53 can regulate a large of number apoptosis regulators. Among these regulators, Bcl-2, Bax and Bak are members of the intrinsic death pathways, which have been shown to participate in lens differentiation. [29][30][31][32][33] Our study demonstrated that HSF4 can upregulate Bax, and participate in the p53-mediated apoptotic program during lens development. Moreover, our study provided evidence that Fas, another p53 target gene and member of the extrinsic death pathway, is also regulated by HSF4. Fas when overexpressed in LEC can trigger cell death and DNA fragmentation. 60 Our data showed that injection of fas mRNA can rescue the denucleation defect caused by hsf4 deletion. Finally, the p53-mediated apoptotic signal pathways activate the executional caspase3, which was found upregulated and had a role during the development of lens in rat, 19,34,36 mouse, 37-39 chicken 30,31,33 and zebrafish. 40 Here, we demonstrated that knockout of hsf4 significantly decreased the activation of caspase3. It has been discovered that, during erythrogenesis, the volume of the nucleus was condensed because of the formation of a caspase3mediated nuclear opening. 61 Erythrogenesis shares some similarities with lens fiber cell differentiation. The same phenomenon has been observed in the hsf4 null zebrafish lens, suggesting that the nuclear condensation in the lens fiber cells may also proceed in a similar way. Besides its control of the above-mentioned pro-apoptotic regulators, HSF4 is also implicated in the regulation of antiapoptosis pathway. Heat shock protein αB-crystallin is a target gene of HSF4 and an important regulator against apoptosis. In mouse lens, αB-crystallin co-localized with procaspase3 in the differentiating secondary fiber cells of the transition zone. 62 Furthermore, the expression of Bax was elevated in the fiber cells, which were undergoing terminal differentiation. 33 Moreover, the co-localization of Bax and αB-crystallin has also been discovered in these fiber cells. By interacting with αB-crystallin, the activation of procaspase3 was repressed and the translocation of Bax from the cytoplasm into the mitochondria was also inhibited. 62 Together, these findings indicate that the regulators including p53, Fas, Bax and caspase3 can form a regulating system with some other target genes of HSF4. This regulating system organized by HSF4 is necessary for the fiber cell differentiation by maintaining a fine balance between pro-apoptosis and anti-apoptosis pathways.
The molecular mechanisms mediating organelle degradation still remain a puzzle and is the focus for the researchers in the field. Our work presented here support the idea that apoptosis-related genes including p53, Fas and Bax are involved in regulating lens fiber cell differentiation. More importantly, we discovered that HSF4 is responsible for activating the p53-mediated apoptosis signaling pathways in the terminal differentiation of lens fiber cell. Our work provided some new clues for revealing why HSF4 mutations can result in the failure in the lens fiber cell differentiation and cause cataract.

Materials and Methods
Zebrafish maintenance and breeding. Our research was approved by the Ethics Committee of Huazhong University of Science and Technology. Zebrafish of AB line were housed in recirculating water system (pH 6.6-7.4, 26-28.5°C) with a daily cycle of 14 h of light and 10 h of dark. We fed the adult zebrafish with fresh brine shrimps three times a day. For the baby fish, we also fed three times a day with live paramecia when they are after 5 dpf, paramecia mixed with brine shrimps after 10 days and only the brine shrimps after 30 dpf. The males and females (1 : 1 or 1 : 2) were separated in the crossing cages with a plastic divider in the early evening before the mating. They were kept undisturbed through the whole night and then we mixed them at about 0900 hours. After spawning, we collected the eggs and bathed them with embryo medium. The embryos were kept in an incubator (28.5°C) for 72 h until the larvae were hatched. During this period, the medium was refreshed and the debris was removed every day.
TALEN construction and mRNA synthesis. The gene sequence information for zebrafish hsf4 (NC_007129) was acquired from NCBI (http://www. ncbi.nlm.nih.gov/). We used the online tools TAL Effector Nucleotide Targeter2.0 (https://tale-nt.cac.cornell.edu/) to design TALENs that target the exon1 of hsf4; the left target sequence was 5′-AGCAATGTGCCCGCTTT-3′ and the right was 5′-CCGGGTCCTCGACCAGAGT-3′. The plasmids of the TALENs were assembled by using the Fast TALE TALEN kit (Sidansai Biotechnology, Shanghai, China) according to the instructions provided by the Company.
Microinjection and genotyping. A pair of TALEN RNAs was mixed and final concentration of each arm was 100ng/μl. Then, the mixed mRNA was injected into one/two-cell-stage embryos. Three days after the injection, 10-15 embryos were collected for extracting genomic DNA. The target site of hsf4 was amplified and the product was digested by T7E1 (ViewSolid Biotech, Beijing, China) to verify the mutation as described in the text.
Frozen section. The eyeballs were isolated from the adult zebrafish and were fixed in the 4% paraformaldehyde (diluted in PBS) immediately. The eyes could be fixed at room temperature for 4-6 h or at 4°C overnight. For the next step, we washed the tissue with PBS for three times and 15 min each time. The sucrose was diluted to a final concentration of 30% in the PBS. The zebrafish eyeballs were dehydrated in the 30% sucrose solution overnight at 4°C. The tissue settled down at the bottom of the tubes when the dehydration was completed. And then, we embedded the eyeball in the OCT compound and then frozen them at − 25°C. Embedded tissues were sliced along the vertical meridian of each eyeball and sectioned each slice to 20 μm thickness. For the small fish that was o10 days of age, the whole body was fixed for the frozen section and each slice was 8 μm thick.
Immunofluorescence and DAPI staining. We performed immunofluorescence on the cryosections and the cultured cells. For the cryosections, they were dried at room temperature to protect them from being detached. For the next step, the sections were washed with PBS for 10 min to remove the OCT compound, permeabilized in PDT (PBS containing 1% DMSO and 0.1% Triton X-100) for 10 min and blocked with 10% normal goat serum in PBDT (PDT containing 1% BSA) for 1 h at room temperature. The Alexa Fluor 568 phalloidin (Invitrogen) was diluted with PBS at the ratio of 1 : 50. The diluted phalloidin was added onto the slides and then incubated for 2 h at 37°C. The slides were washed three times with PBS. DAPI was used to label the nucleus at the concentration of 5 μg/ml. The sections were then rinsed three times with PBS, and mounted with a glycerol-based liquid mountant under coverslips. For the cultured cells, we washed the cells with PBS for three times to remove the medium and fixed them in 4% paraformaldehyde and permeabilized with 0.5% Triton X-100 for 15 min. The cells were blocked for 1 h at the room temperature with 5% BSA in PBS and then incubated with p53 primary antibody (1 : 100, Proteintech, Wuhan, China) overnight at 4°C. Afterward we removed the primary antibody through washing them with PBS for three times. The cells were incubated with the secondary antibody conjugated with Alexa Fluorescence 568 (1 : 1000, Invitrogen, Carlsbad, CA, USA) in 37°C for 1 h. Nuclei were stained with DAPI (1 : 5000, Sigma, St. Louis, MO, USA). Fluorescence images were acquired using a confocal laser-scanning microscope (FluoView FV1000 confocal microscope, Olympus Imaging, Tokyo, Japan).
Transmission electron microscopy. Adult zebrafish eyes were isolated and fixed in 2.5% glutaraldehyde in 0.1 M PBS buffer (pH 7.0) overnight at 4°C. After three washes (15 min each) with 0.1 M PBS buffer, the eyes were further fixed in 1% osmium tetroxide in 0.1 M PBS buffer for 2 h at room temperature. After three washes (15 min each) with 0.1 MPBS buffer, the eyes were dehydrated in 50, 70, 80, 90, 95 and 100% ethanol successively (20 min each) and incubated in acetone for 20 min at room temperature. The eyes were treated with 50% (1 h), 75% (3 h) and 100% (overnight) epoxy resin (mixed with acetone, v/v), and then heated at 70°C overnight. Embedded eyes were sliced to ultrathin sections (70 nm) using an Reichert-Jung ultramicrotome (Leica, Wetzlar, Germany). Sections were stained with 3% uranyl acetate and 3% lead citrate for 15 min and were visualized with a transmission electron microscope system (HT7700, Hitachi, Tokyo, Japan).
Western blot. For the cultured cells and the lens isolated from the eyeballs, both were lysed in RIPA cell lysis buffer (Beyotime, Shanghai, China) with cocktail protease inhibitor (Roche, Basel, Switzerland). After the ultrasonic decomposition and centrifugation, the protein extraction was completed. Protein concentration was determined using the BCA Protein Assay Kit (Beyotime). We mixed the protein lysates with loading buffer and boiled them for 10 min at 100°C. And then, we placed the protein samples on the ice for 3 min before loading. The protein samples were separated by 12% SDS-PAGE and transferred to nitrocellulose membranes (Millipore, Danvers, MA, USA). Then, we blocked the membranes in the 5% skim milk dissolved in TBST buffer (20 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20, pH 7.6) for 1 h at the room temperature. For the next step, we washed the membranes with TBST buffer and incubated them with the diluted solution of primary antibodies overnight at 4°C with gentle agitation. And then we washed the membranes with TBST buffer three times for 5 min each time. To visualize the result, we incubated the membranes in HRP-conjugated secondary antibodies (1 : 20 000; Thermo, Wilmington, DE, USA) for 2 h at room temperature. After incubation, the membranes were washed for another three times (5 min each time) with TBST buffer. The signals were detected using a ChemiDoc XRS (Bio-Rad Laboratories, Berkeley, CA, USA), with Super Signal Sensitivity Substrate (Thermo), and quantified with Quantity One software (Bio-Rad Laboratories, Berkeley, CA, USA).
Statistical analysis. All experiments were repeated at least three times. Significance was determined by two-tailed Student's t-test.