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A SCID Mouse Model To Evaluate the Efficacy of Antivirals against SARS-CoV-2 Infection

ABSTRACT Ancestral severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lacks the intrinsic ability to bind to the mouse ACE2 receptor, and therefore establishment of SARS-CoV-2 mouse models has been limited to the use of mouse-adapted viruses or genetically modified mice. Interestingly, some of the variants of concern, such as the Beta B.1.351 variant, show an improved binding to the mouse receptor and hence better replication in different wild-type (WT) mouse species. Here, we describe the establishment of a SARS-CoV-2 Beta B.1.351 variant infection model in male SCID mice as a tool to assess the antiviral efficacy of potential SARS-CoV-2 small-molecule inhibitors. Intranasal infection of male SCID mice with 105 50% tissue culture infective doses (TCID50) of the Beta B.1.351 variant resulted in high viral loads in the lungs and moderate signs of lung pathology on day 3 postinfection. Treatment of infected mice with the antiviral drugs molnupiravir (200 mg/kg, twice a day [BID]) or nirmatrelvir (300 mg/kg, BID) for 3 consecutive days significantly reduced the infectious virus titers in the lungs by 2 and 3.9 log10 TCID50/mg of tissue, respectively, and significantly improved lung pathology. Together, these data demonstrate the validity of this SCID mouse Beta B.1.351 variant infection model as a convenient preclinical model for assessment of potential activity of antivirals against SARS-CoV-2. IMPORTANCE Unlike the ancestral SARS-CoV-2 strain, the Beta (B.1.351) variant of concern has been reported to replicate to some extent in WT mice (C57BL/6 and BALB/c). We demonstrate here that infection of SCID mice with the Beta variant resulted in high viral loads in the lungs on day 3 postinfection. Treatment of infected mice with molnupiravir or nirmatrelvir for 3 consecutive days markedly reduced the infectious virus titers in the lungs and improved lung pathology. The SARS-CoV2 SCID mouse infection model, which is ideally suited for antiviral studies, offers an advantage in comparison to other SARS-CoV2 mouse models, in that there is no need for the use of mouse-adapted virus strains or genetically modified mice. Mouse models also have advantages over hamster models because (i) lower amounts of test drugs are needed, (ii) more animals can be housed in a cage, and (iii) reagents to analyze mouse samples are more readily available than those for hamsters.

attachment to cellular angiotensin-converting enzyme 2 (ACE2) (4). Since SARS-CoV-2 binds efficiently to the hamster ACE2 (5), Syrian hamsters are considered one of the best small animal models available for SARS-CoV-2. On the other hand, the spike protein of the ancestral SARS-CoV-2 strain lacks the intrinsic ability to efficiently bind to the murine ACE2 (5), and hence this strain has limited replication in wild-type (WT) mice. Consequently, alternative strategies have been developed to allow the establishment of mouse models for SARS-CoV-2. One of these strategies is adaptation of the virus in murine lung tissues to enhance binding capacity to the murine ACE2 (6,7). Other strategies focused on introduction of human ACE2 in wild-type mice either by transduction of adenovirus or an adeno-associated virus that expresses human ACE2 (8) or by using genetically modified human ACE2 transgenic (9) or humanized mice (10). Unlike the ancestral strain, some of the evolved SARS-CoV-2 VoCs proved to carry spike protein mutations, mainly the mutation cause by an N-to-Y change at position 501 (N501Y), that enable efficient binding to the murine ACE2 and hence better replication in WT mice (11,12). Besides the N501Y mutation, the spike of the Beta B.1.135 variant carries the K417N mutation, which was previously reported in a virulent mouse-adapted SARS-CoV-2 variant (13). Several studies have shown the ability of the Beta variant to replicate to some extent in WT mouse strains such as C57BL/6 (11,12) and BALB/c (14,15). Here, we wanted to explore whether the Beta SARS-CoV-2 variant replicates more efficiently in severe combined immune deficient (SCID) mice than in wild-type mice and whether, in such a case, SCID mice can be used to develop a sufficiently robust infection model to study the efficacy of small-molecule inhibitors of SARS-CoV-2 infection.

RESULTS
First, a small pilot study was performed to assess the efficiency of replication of the Beta (B.1.351) SARS-CoV-2 variant in male SCID mice in comparison to replication in immunocompetent male BALB/c and C57BL/6 mice. All mice (n = 9 per strain) were infected with 10 5 50% tissue culture infective doses (TCID 50 ) of the Beta variant. Based on previous studies where the Beta variant was used to infect wild-type mice (14-16), we selected day 3 postinfection (p.i.) as the endpoint at which all animals were euthanized and lungs were collected to quantify the infectious virus titers. The infectious virus titers in the lungs of infected SCID mice (median 3.28 Â 10 4 TCID 50 /mg lung tissue) was markedly higher than that observed in the lungs of infected BALB/c mice (median 3.26 Â 10 3 TCID 50 /mg lung tissue; P = 0.22 [nonsignificant]) and C57BL/6 mice (median 2.05 Â 10 3 TCID 50 /mg lung tissue; P = 0.0098) (Fig. 1A). A minor weight loss was observed on the sacrifice day for infected SCID and BALB/c mice (average body weight change of 21.7% and 20.09%, respectively) but not for infected C57BL/6 mice (average body weight change of 0.5%) (Fig. 1B). Histological examination of lungs from all infected mice showed some signs of bronchopneumonia, peribronchial inflammation, and perivascular inflammation (Fig. 1C). The average cumulative lung pathology scores were 4.3, 3.5, and 4 for infected SCID, BALB/c, and C57BL/6 mice, respectively (Fig. 1C).
Next, we explored the kinetics of replication of the Beta variant in SCID mice. To that end, 7-to 9-week-old male SCID mice were infected intranasally with 10 5 TCID 50 of the Beta variant. Five mice were euthanized at day 1 p.i., and then from days 3 through 7 p.i., 10 animals were euthanized per time point and lungs were collected to quantify the infectious virus titers. The highest infectious virus titers were observed at day 1 and day 3 p.i. (Fig. 2A). From day 4 p.i. onwards, the infectious virus titers in the lungs were significantly lower than those observed at day 3 p.i. (Fig. 2A). A minor weight loss was observed from day 1 to day 3 p.i. (average body weight change of 20.09% to 21.1%), after which animals started to gain weight normally (average body weight change of 4% on day 4 p.i.) (Fig. 2B). When a group of 5 infected mice were monitored up to 14 days p.i., no weight loss or any signs of morbidity were observed in this group (average body weight change of 13% on day 14 p.i.). Histological examination of lungs from infected mice showed the highest pathology score on day 3 p.i., with a median cumulative pathology score of 4.5, and pathology scores significantly dropped from day 6 p.i. (Fig. 2C). Hematoxylin and eosin (H&E)stained images of the lungs from infected mice at day 3 p.i. revealed mild signs of peribronchial inflammation, significant perivascular inflammation, and intra-alveolar hemorrhage (Fig. 2D).
If the infectious virus detected at day 3 postinfection represented actively replicating virus, it should have been possible to suppress replication by treating the animals with antiviral drugs. We therefore assessed the potential antiviral efficacy of two clinically relevant SARS-CoV-2 inhibitors, i.e., molnupiravir (EIDD-2801) and nirmatrelvir (PF-332), against Beta variant replication in SCID mice. Briefly, male SCID mice were treated twice daily by oral gavage with either vehicle, molnupiravir (200 mg/kg), or nirmatrelvir (300 mg/kg) for three consecutive days, starting from the day of infection with the Beta variant (Fig. 3A). Mice were euthanized at day 3 p.i. for collection of lung tissues. A significant reduction of viral RNA loads was observed in the molnupiravir-treated (0.8 log 10 genome copies/mg tissue; P = 0.011) and nirmatrelvir-treated (2.8 log 10 genome copies/mg tissue; P , 0.0001) groups compared to the vehicle control ( Fig. 3B). Moreover, treatment of mice with molnupiravir and nirmatrelvir significantly reduced the infectious virus titers in the lungs by 2 log 10 (P = 0.0001) and 3.9 log 10 (P , 0.0001) TCID 50 /mg tissue, respectively, compared to the vehicle-treated group (Fig. 3C). No infectious virus titers were detected in 4 (out of 14) and 8 (out of 14) animals in the molnupiravir-and nirmatrelvir-treated groups, respectively (Fig. 3C). A significant improvement of lung histopathology scores was also observed in both the molnupiravir-treated (P = 0.018) and nirmatrelvirtreated (P = 0.0005) groups (Fig. 3D). No significant weight loss or clinical signs of adverse effects were observed in the compound-treated groups (Fig. 3E).

DISCUSSION
The emergence of SARS-CoV-2 VoCs has raised a lot of concern as these variants displayed the ability to escape vaccine-induced or naturally acquired immunity and to transmit faster than the ancestral strains. In addition, some of these variants have acquired certain mutations in the spike protein that allow them to expand their host species (2,12). The Beta variant (B.1.351 or 501Y.V2) was first reported in South Africa in October 2020 (17). The Beta variant has acquired three mutations in the receptor binding domain (RBD), namely, N501Y, K417N, and E484K, in addition to other mutations in the spike and nonstructural proteins (2). Among these mutations, the N501Y mutation (also present in the alpha variant) has been previously described in mouse-adapted viruses and proven to play an important role in increasing the affinity to the mouse ACE2 receptor (6). The K417N mutation has also previously been reported in a virulent mouse-adapted SARS-CoV-2 variant (13). In a pseudotypebased entry assay, the pseudoviruses carrying the Beta variant of the spike protein attached more efficiently to the mouse ACE2 receptor than the alpha variants, suggesting that the K417N and E484K mutations in the RBD of the Beta variant may further enhance the binding to the mouse receptor (11). Recently, a comparative infection study in BALB/c mice revealed that the Beta variant replicates more efficiently than the alpha or delta variant (15).
Here, we wanted to assess the infectivity of the Beta SARS-CoV-2 variant in an immunodeficient mouse model, i.e., SCID mice, with the aim to develop a robust SARS-CoV-2 mouse infection model for preclinical evaluation of potential antivirals. So far, the hamster SARS-CoV-2 infection model has been regarded as the best model to study the effect of antiviral agents, yet use of mice would facilitate such studies. We selected SCID mice, as these animals are severely deficient in functional B and T lymphocytes and, therefore, they are believed to be more susceptible to viral infections than immunocompetent mice. Indeed, in our pilot infection study, the infectious virus titers of the Beta variant in the lungs of infected SCID mice on day 3 p.i. were significantly higher than that observed in the lungs of immunocompetent BALB/c (1 log 10 higher) or C57BL/6 mice (1.2 log 10 higher) (Continued on next page) that were infected in parallel. Viral persistence in the lungs of SCID mice was observed in most of the infected animals up to 7 days p.i. However, the infectious virus titers dropped significantly beyond day 3 p.i. Therefore, day 3 p.i. was selected as the endpoint for antiviral testing. Nirmatrelvir (PF-332; Pfizer) is a potent inhibitor of the main protease M pro (or 3CL protease) of SARS-CoV-2 and other coronaviruses (18). Paxlovid (nirmatrelvir and ritonavir tablets, copackaged for oral use) has been authorized by the FDA and EMA as well as by other regions. Molnupiravir (Lagevrio; EIDD-2801; Merck) is the orally bioavailable prodrug of the ribonucleoside analog N4-hydroxycytidine (NHC; EIDD-1931), which was initially developed for influenza (19) and has now also been approved by several countries and regions for the treatment of COVID-19.
We previously showed that molnupiravir (EIDD-2801) and nirmatrelvir (PF-332) significantly inhibit the replication of the Beta variant in Syrian hamsters (20,21). Therefore, we used these two antiviral drugs to validate the SCID mouse, Beta variant infection model for antiviral studies. Treatment of Beta variant-infected SCID mice for 3 consecutive days with molnupiravir (200 mg/kg, twice a day [BID]) or nirmatrelvir (300 mg/kg, BID) significantly reduced viral loads in the lungs of infected mice, with a potency close to that observed against the same variant in our Syrian hamster model (where the endpoint was at 4 days postinfection) (20,21). An improvement in lung pathology scores was also observed in the molnupiravir-and nirmatrelvir-treated SCID mice compared to the vehicle-treated mice. Thus, the SCID mouse, Beta variant infection model may serve as a useful tool to assess the in vivo efficacy of antiviral molecules against SARS-CoV-2.
It is surprising that infected SCID mice seem to control the infection by day 4 postinfection. Moreover, monitoring a group of infected mice up to 14 days p.i. did not reveal any morbidity signs or weight loss over time. Typically, infection of SCID mice with viruses (those that are able to replicate in mice) results in a lethal infection (22)(23)(24).
The advantages of using this mouse model over other SARS-CoV-2 mouse models for initial in vivo evaluation of antivirals include the use of a real clinical isolate without the need to use mouse-adapted strains or genetically modified animals. Moreover, this mouse model has some advantages over bigger rodents, such as hamsters, as roughly 4-to 6-fold less of the test drug is needed for the in vivo efficacy studies (average weight of a hamster is 80 to 120 g, versus 20 g for mice), which will save a lot of material; this is of particular important in cases of highly priced or difficult-to-synthesize compounds. The more convenient housing conditions, as up to 5 mice can be cohoused in one cage, versus 2 hamsters per cage, are important for the capacity of the high-level biosafety animal facility. Finally, more reagents are available for analysis of mouse samples than for hamster ones. Consequently, such a model will enable testing more compounds in a shorter period of time. On the other hand, a limitation of this model is that, unlike for hamsters, mice are only susceptible to the Beta variant. Since small-molecule inhibitors should have equipotent activity against all variants, this will be of limited concern for studies with such drugs. However, for testing of therapeutic antibodies, infection models (in hamsters) with the different VoCs will still be needed. Likewise, for vaccine studies fully immunocompetent animals are needed; SCID mice are not useful for this purpose. Therefore, this SCID mouse, Beta variant infection model will be advantageous mainly for the evaluation of small-molecule inhibitors of SARS-CoV-2 replication.
Beta SARS-CoV-2 variant infection in different mouse species. In brief, 7-to 9-week-old male SCID mice (CB-17/Icr-Prkdc scid/scid /Rj; Janvier Laboratories), male BALB/c mice (internally bred), and C57BL/6 mice (internally bred) were anesthetized with isoflurane and inoculated intranasally with 40 mL containing 10 5 TCID 50 SARS-CoV-2 Beta variant (day 0). Mice were housed in individually ventilated cages with a maximum of five mice per cage and monitored daily for weight changes and any clinical signs. At day 3 p.i., 9 animals were euthanized by intraperitoneal injection of 100 mL Dolethal (200 mg/mL sodium pentobarbital; Vétoquinol SA), and lungs were collected and viral RNA and infectious virus were quantified by reverse transcription-quantitative PCR (RT-qPCR) and endpoint virus titration, respectively. The left lungs were fixed in 4% formaldehyde for histopathological analysis.
Treatment regimen. Male SCID mice were treated by oral gavage with either the vehicle (n = 18) or molnupiravir (n = 14) at 200 mg/kg or nirmatrelvir (n = 14) at 300 mg/kg, twice daily starting from day 0, just before infection with the Beta variant as described in the previous section. All treatments were continued for 3 consecutive days (i.e., until day 2 p.i.). Mice were monitored for appearance, behavior and weight. At day 3 pi, mice were euthanized and lungs were collected and viral RNA and infectious virus were quantified by RT-qPCR and endpoint virus titration, respectively. The left lungs were fixed in 4% formaldehyde for histopathological analysis.
SARS-CoV-2 RT-qPCR. Lung tissues were collected after sacrifice and were homogenized using bead disruption (Precellys) in TRK lysis buffer (EZNA total RNA kit; Omega Bio-tek) and centrifuged (10,000 rpm, 5 min) to pellet the cell debris. RNA was extracted according to the manufacturer's instructions. RT-qPCR was performed on a LightCycler96 platform (Roche) using the iTaq universal probes one-step RT-qPCR kit (Bio-Rad) with N2 primers and probes targeting the nucleocapsid (26). Standards of SARS-CoV-2 cDNA (IDT) were used to express viral genome copies per milligram of tissue.
Endpoint virus titrations. Lung tissues were homogenized using bead disruption (Precellys) in minimal essential medium and centrifuged (10,000 rpm, 5 min, 4°C) to pellet the cell debris. To quantify infectious SARS-CoV-2 particles, endpoint titrations were performed on confluent Vero E6 cells in 96-well plates. Viral titers were calculated by the Reed and Muench method (27) using the Lindenbach calculator and were expressed as the TCID 50 per milligram of tissue.
Histology. For histological examination, the lungs were fixed overnight in 4% formaldehyde and embedded in paraffin. Tissue sections (5 mm) were analyzed after staining with hematoxylin and eosin and scored blindly for lung damage by an expert pathologist. The scored parameters, to which cumulative scores of 1 to 3 were attributed, were the following: congestion, intra-alveolar hemorrhagic, apoptotic bodies in bronchus wall, necrotizing bronchiolitis, perivascular edema, bronchopneumonia, perivascular inflammation, peribronchial inflammation, and vasculitis.
Ethics. Housing conditions and experimental procedures were approved by the ethics committee of animal experimentation of KU Leuven (license P001/2021).
Statistics. GraphPad Prism (GraphPad Software, Inc.) was used to perform statistical analysis. Statistical significance was determined using the nonparametric Kruskal-Wallis test. P values of ,0.05 were considered significant.
European Union's Horizon 2020 Research and Innovation program and EFPIA and from the Bill & Melinda Gates Foundation, Global Health Drug Discovery Institute, University of Dundee. The content of this publication only reflects the authors' views, and the JU is not responsible for any use that may be made of the information this publication contains. R.A., C.S.F., S.J.F.K., and J.N. designed the studies; R.A., S.J.F.K., and R.B. performed studies. R.A. and B.W. analyzed data; R.A. made the graphs; B.W., D.J., and J.N. provided advice on the interpretation of data; R.A. and J.N. wrote the paper. S.D.J. provided essential reagents; R.A., C.S.F., S.J.F.K., and J.N. supervised the study; L.V., D.J., and J.N. acquired funding.
We have no conflicts to declare.