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On/off control of biomolecular motors in a microfluidic device

We have successfully realized an on/off control of biomolecular linear motors in a polydimethyl siloxane (PDMS) chamber sealed with a cover glass. A linear biomolecular motor system, microtubule-kinesin system, is controlled by injecting the optimized concentrations of adenosine triphosphate (ATP) for the activation and hexokinase for the stoppage. Not only control from on to off or off to on, the repetitive injection of ATP and hexokinase proves that kinesin molecules can move and stop alternatively once they bind to microtubules.