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The roles of LPA2 on erythrocyte and thrombocyte differentiation in zebrafish

Lysophosphatidic acid (LPA) is a simple lipid growth factor regulates cell proliferation, migration, and survival. It binds to a family of trans‐membrane G‐protein‐coupled receptors. In our previous study, LPA receptors were expressed in hematopoietic stem cells (HSCs) and activation of LPA3 leads to erythropoiesis. However, the roles of LPA2 on erythrocyte differentiation remain unclear. During hematopoiesis processes, erythrocytes and thrombocytes have a common precursor. Therefore, we also attempted to determine whether LPA2 regulates thrombocyte differentiation in zebrafish. Anti‐sence morpholino oligonucleotide of LPA2 was injected into zebrafish embryos at different stages. We used o‐dianisidine staining to determine the levels of hemoglobin expression and Real‐Time PCR for mRNA expression of Gata1, an erythropoietic marker. The expression levels of CD41, a thrombocyte marker, were determined by Real‐Time PCR and in situ hybridization. Our results demonstrated that knocked down of LPA2 enhanced the expression of CD41 while no effect was observed for hemoglobin and Gata1 expression. Therefore, our results suggested that activation of LPA2 may decrease thrombocyte differentiation.