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Effect of Sterol Inhibitors on the Incorporation of 14C-Isopentenyl Pyrophosphate into Artemisinin by a Cell-Free System from Artemisia annua Tissue Cultures and Plants.

A cell-free system from ARTEMISIA ANNUA L. shoot cultures and plants (shoots and roots) which is capable of incorporating (14)C-isopentenyl pyrophosphate ( (14)C-IPP) into artemisinin has been developed. The sterol inhibitors miconazole, AMO 1618, CCC, and MER 29 increased both the incorporation of (14)C-IPP into artemisinin by cell-free extracts and the production of artemisinin in shoot cultures of ARTEMISIA ANNUA.