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[Preparation and identification of monoclonal antibodies against snake venom C-type lectin like protein Agkisacutacin].

AIM
To prepare and identify the antibodies against snake venom C-type lectin like protein Agkisacutacin.


METHODS
A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot.


RESULTS
Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained.


CONCLUSION
The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.