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cDNA‐derived amino‐acid sequence of a land turtle (Geochelone carbonaria) β‐chain hemoglobin

The cDNA sequence encoding the turtle Geochelone carbonaria β‐chain was determinated. The isolation of hemoglobin mRNA was based on degenerate primers' PCR in combination with 5′‐ and 3′‐RACE protocol. The full length cDNA is 615 bp with the ATG start codon at position 53 and TGA stop codon at position 495; The AATAAA polyadenylation signal is found at position 599. The deduced polypeptyde contains 146 amino‐acid residues. The predicted amino acid sequence shares 83% identity with the β‐globin of a related specie, the aquatic turtle C. p. belli. Otherwise, identity is higher when compared with chicken β‐Hb (80%) than with other reptilian orders (Squamata, 69%, and Crocodilia, 61%). Compared with human HbA, there is 67% identity, and at least three amino acid substitutions could be of some functional significance (Glu43β‐→Ser, His 116β→Thr and His143β→Leu). To our knowledge this represents the first cDNA sequence of a reptile globin gene described.