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The complete chloroplast genome of Asarum pulchellum Hemsl. (Aristolochiaceae: Asarum L.)

Abstract Asarum pulchellum Hemsley 1890, belonging to Aristolochiaceae family, is a kind of folk herb resource with certain toxicity. In this study, we acquired the complete chloroplast genome of A. pulchellum for its accurate species identification and phylogenetic analysis. The genome is 177,905 bp in size with a typical circular quadripartite structure, consisting of a large single-copy region, a small single-copy region and a pair of inverted repeats. In total, 136 genes were annotated, including 92 protein-coding genes, 36 tRNA genes and eight rRNA genes. In the phylogenetic analysis, A. pulchellum and A. sieboldii formed a sister clade. The chloroplast genome helps further studies of taxonomy and genetic evolution of Aristolochiaceae family.

Asarum pulchellum Hemsl., namely Changmao Xixin in Chinese, is a perennial herbaceous plant belonging to Asarum genus of Aristolochiaceae family (Kelly 2001). In China, it is mainly distributed in Hubei, Sichuan, Yunnan and Anhui provinces (Lin et al. 1995). A. pulchellum contains multiple medicinal components, with the effects of lung warming, dehumidification and pain relief, which can be used to treat cold cough, rheumatic arthralgia, stomachache and other ailments (Kopyt Ko et al. 2013). It is used locally as an endemic herbal resource. However, like other species in Asarum genus, A. pulchellum also features toxic compounds, e.g., safrole and aristolochic acid, which can cause nephrotoxicity and carcinogenicity when ingested inappropriately (Michl et al. 2017;Han et al. 2019). There are approximately 90 species in Asarum genus, whose chemical constituents are divergent (Wu et al. 2021). The misuse of raw materials may adversely affect the safety and effectiveness of their clinical application. So far, there is still a lack of research on A. pulchellum, and it is urgent to establish a method for the accurate identification of A. pulchellum to ensure its correct use.
This study aims to determine the complete chloroplast genome sequence of A. pulchellum. On one hand, it can be considered as a DNA super barcode for species identification of A. pulchellum to ensure the accuracy of the original medicinal materials and guarantee the effectiveness and security of its clinical application; on the other hand, it helps gain a better understanding of the genetic and evolutionary relationship of Asarum genus. The complete chloroplast genome of A. pulchellum (GenBank accession number: MZ440306) is 177,905 bp in length, and the total GC content is 37.75%. The genome features a typical annular quadripartite structure, including a large single-copy (LSC) region and a small single-copy (SSC) region of 90,885 bp and 8,920 bp respectively, which are separated by a pair of inverted repeats (IRa and IRb) of 39,050 bp. A total of 136 genes were annotated within the chloroplast genome, including 92 protein-coding genes, 36 tRNA genes and eight rRNA genes. Thereinto, 26 genes (15 protein-coding genes, seven tRNAs and four rRNAs) are duplicated in IR regions.
To explore the phylogenetic relationship of A. pulchellum with other related taxa, a maximum-likelihood (ML) tree was constructed using IQ-TREE software with TVM þ FþR6 nucleotide substitution model (Nguyen et al. 2015). Eighteen complete chloroplast genomes were used in the ML tree ( Figure  1), including seven Asarum species, eight Aristolochia species and three outgroup species (Ephedra sinica, E. intermedia and E. equisetina). The 15 species from Aristolochiaceae clustered together, and the Asarum genus and Aristolochia genus were separated. A. pulchellum and A. sieboldii formed a sister clade. Therefore, the complete chloroplast genome sequence of A. pulchellum is an essential reference resource for the research of phylogeny and population genetics of Aristolochiaceae family.

Disclosure statement
No potential conflict of interest was reported by the author(s).

Data availability statement
The genome data that support the findings of this study are available in GenBank of NCBI (https://www.ncbi.nlm.nih.gov/) with the accession NO.