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The morphological effects in pig liver after Nd-YAG laser resection.

The morphological effects of liver resection in 15 pigs performed with a Nd-YAG laser beam are reported. A regular cut surface was obtained and overlayed an ischemic layer which was thinnest at a 80 W power output and a short exposure time. Hemostasis was satisfying when the encountered vessels did not exceed 4,5 mm in diameter. Semi-thin and electron microscopic examination showed "ghost" cells in the layer of coagulation necrosis, shrunken hepatocytes and red blood cells in oedematous layer and "swollen" mitochondria in the deepest layer. Clamping of the hepatic pedicle - increasing local hyperthermia by suppression of cooling vascular inflow - majorated the depth of cellular alterations and the intensivity of the post-operative inflammatory reaction. Numerous biliary ductules and active hepatocytes meant parenchymal regeneration. Good healing evolution free of complications was observed after a one year's follow up.