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Reduced Leucocyte Alkaline Phosphatase Activity and Decreased NBT Reduction Test in Induced Iron Deficiency Anaemia in Rabbits

Summary. Iron deficiency anaemia was induced in rabbits by repeated bleeding. The leucocyte alkaline phosphatase (LAP) of 26 ± 28 units was significantly reduced compared with control values of 233 ± 36 units (P < 0.001). Leucocyte NBT reduction was also diminished, both in Hanks solution (P < 0.01) and in autologous serum (P < 0.001). After administration of iron, these values returned to normal.