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Review: UV Derivative Spectroscopic Studies to Characterize the Structure, Biotransformation, and Mechanism of Action of Testosterone

Testosterone has been and still is being thoroughly studied due to its importance since its discovery in 1935. Since then, numerous derivatives of testosterone have been synthesized, with the goals of prolonging its biological activity in vivo, producing orally active androgens, and developing products, commonly referred to as anabolic androgenic steroids that are more anabolic and less androgenic than the parent molecule. In the present manuscript, we review the structure, biotransformation, and mechanism of action of testosterone There are many aspects of the study on testosterone, but here we have confined our literature survey mainly to the following areas: (1) UV Spectroscopic studies, (2) hydrogen bonding and association with other solute molecules and solvents, and (3) charge transfer (CT) complexes. We also focused on UV-derivative spectroscopy studies of testosterone because the higher derivative transformation of data is now a well-established technique, which, when combined with least squares deconvolution, offers a powerful approach to quantitative analysis of testosterone in environmental and industrial applications. The transformation of these databases to the derivative domain will enable additional parameters to be employed in developing archive retrieval techniques for the identification of unknown drugs, metabolites, toxic agents, and endogenous materials.