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Position-referenced microscopy: regions of interest localization and subpixel image comparison by means of pseudo-random patterns embedded in cell culture boxes

This work presents micro-grids integrated to cell culture boxes. These grids allow the systematic registration of the position of a zone observed by optical microscopy in a such way that it is possible to find it again easily for new observations for instance after culture on drug injection. The position knowledge allows also the numerical superimposition of recorded images in a common position reference system with a sub-pixel precision. It become thus straightforward to perform a site by site analysis of the possible evolutions that may have occurred in the biological medium.