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[Human T cell and monocyte modulating activity of Rhizoma typhonii in vitro].

OBJECTIVE
To study the immunological modulating activity of Rhizoma typhonii (RT) on lymphocytes in vitro.


METHODS
Effect of RT extract (RTE) on mice spleen cells and human lymphocytes proliferation was detected by sheep erythrocytes rosette forming test after lymphocytes had been isolated and purified. The activity of human killer cells and natrral killer (NK) cells was tested using 51 Cr free test. The production of cytokine of macrophages was examined using ELISA test and biological test and the phagocytic function of macrophage on tumor cell was determined using 3H incorporation assay. The chemical ingredients of RT were analyzed using chromatography and the molecular weight of their active principle was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis technique (SDS-PAGE) method.


RESULTS
RTE could markedly stimulate the mice spleen cells and human lymphocytes to proliferate in a dose-effect dependent manner; the effector cell of RTE is T cells. RTE enhanced human killer cell, the allo-antigen specific cytotoxic T lymphocytes (CTL) activity and the nonspecific activity of NK cells. Furthermore, RTE stimulated macrophage to produce tumor necrosis factor (TNF-alpha) and interleukin-1 (IL-1), and enhanced phagocytic activity of macrophage to tumor cells. The elution of RTE from chromatography showed that the active principle of RT was glycoproteins in nature and had an apparent molecular weight of 66 kDa.


CONCLUSION
RTE has immunoenhancing activity to human T cell and macrophage, through stimulating the killer cell and phagocytosis of tumor cell and allo-antigen, which could be used clinically for modulating immune responses and for treating tumor and other diseases.