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The essential oils component p-cymene induces proton leak through Fo-ATP synthase and uncoupling of mitochondrial respiration

Essential oils can be used as antimicrobial, antioxidant, and anticarcinogenic agents or to preserve and give flavors to foods. The activity of phenolic-rich essential oils has been observed in fractions containing thymol and carvacrol which show synergistic effects with their precursor p-cymene. Their mode of action is related to several targets in the cell but specific mechanisms of activity and cytotoxic effects remain poorly characterized. Given the importance of mitochondria for cellular functions and their critical role in a vast number of diseases, this work evaluated the effects of p-cymene on mitochondrial functions. It was observed that p-cymene did not change the oxygen consumption by respiratory chain (state 2 respiration). However, p-cymene decreased the mitochondrial membrane potential (Δψ), depressed the rate of ADP phosphorylation (state 3), and stimulated the oxygen consumption after phosphorylation of ADP (state 4). The respiratory control ratio (state 3/state 4) was decreased as a consequence of the inhibition of state 3 and stimulation of state 4 respiration but the ADP/O index remained unaltered as well as the mitochondrial Ca2+ fluxes. Moreover, p-cymene did not induce mitochondrial membrane disruption but depressed the Δψ, and the stimulatory effect observed on state 4, similar to the effect observed on state 2 respiration plus ATP, was inhibited by oligomycin. These effects suggest that p-cymene allows a proton leak through the Fo fraction of the phosphorylative system, changing the mitochondrial proton motive force and ATP synthesis capacity. Therefore, these data suggest mitochondria as a target for p-cymene toxicity action mechanisms.


Introduction
p-Cymene (1-isopropyl-4-methylbenzene) ( Figure 1) is a natural hydrocarbon that is a component of essential oils, ie, natural products extracted from vegetable materials. For example, the essential oils obtained from oregano and thyme, traditional Mediterranean spices, are characterized by a very high content of monoterpenes such as p-cymene (trace to 52%), γ-terpinene (2%-52%), thymol (trace to 64%), and carvacrol (1%-80%). 1,2 However, p-cymene occurs in the oils of many other gymnospermic and angiospermic plants. It is metabolized by the liver and the major p-cymene metabolite is cuminyl alcohol. 3 Furthermore, formation of p-cymene as a dead-end metabolite from certain nonaromatic monoterpenes has been observed in anoxic enrichment cultures 4,5 and degradation has been also demonstrated with anaerobic and aerobic bacteria. 6 In vitro studies have demonstrated that the monoterpene p-cymene can be used as an antimicrobial against the Gram-positive bacterium Staphylococcus aureus and the Gram-negative Escherichia coli, 7,8 and synergistic effects have been observed between both monoterpenes carvacrol and thymol, main components of some essential oils and responsible for their activity, 9 and their precursor p-cymene. [10][11][12] Furthermore, different studies have shown that p-cymene and its derivative monoterpenes are efficient antioxidant and anticarcinogenic agents, and they are approved in the United States and Europe to preserve and to give specific flavors to foods and feeds. 11 However, p-cymene has been reported to be less effective against food-related pathogens.
The precise target of the antimicrobial action of p-cymene and derivatives has not yet been fully established but it has been reported that the mode of action involves several targets in the cell. p-Cymene has been shown to have lipolytic properties. In fact, it has been reported that the antimicrobial effects of p-cymene and derivatives carvacrol, thymol, and γ-terpinene may result, partially at least, from a gross perturbation of the lipid fraction of the plasmic membrane, acting by disrupting the cytoplasmic membrane, 7 with loss of its high impermeability for protons and bigger ions. The disturbance of membrane integrity compromises its functions not only as a barrier but also as a matrix for enzymes and as an energy transducer. 13 Changes in the fatty acid composition of bacterial cell membranes (an increase in unsaturated fatty acids) and changes in membrane fluidity have been observed. 14 Therefore, the hydrophobicity enables them to partition in the lipids of the cell membrane and mitochondria, rendering them permeable and leading to leakage of cell contents. In addition to the effects on the membrane integrity and transmembrane potential, treatment with this monoterpenes induced depletion of the intracellular ATP levels when bacterial cells were exposed to sublethal concentrations. 15 Moreover, recent studies have found that these compounds are effective against Leishmania. 16 The mechanism of leishmanicidal action of these compounds could be related to their interference in the mitochondrial bioenergetic processes of Leishmania.
The range of concentrations of p-cymene used as antimicrobial additives in food and feed (0.25-1.25 mM) 12,17 remains poorly characterized relative to specific mechanisms involved in the antimicrobial action and cytotoxic effects in humans. Moreover, the mechanisms of action proposed are not specific to microorganism metabolic pathways and could affect the mammalian cells. Thus, to ensure the safe and successful use of monoterpenes essential oil components as food additives and therapeutical drugs, toxicity studies with these compounds should be performed. Given the importance of mitochondria to basic cellular functions, including production of ATP, their role in controlling pathways of intracellular Ca 2+ homeostasis, 18 apoptotic and necrotic cell death, 19 and the critical role of mitochondrial impairment in a vast number of disorders, 20,21 the aim of this work was to evaluate whether p-cymene modulates mitochondrial functions. Although, it is difficult to extrapolate the data obtained with mitochondrial studies to the in vivo situation in humans, the concentrations of p-cymene used in this study (#100 nmol/mg of protein or 25-100 µM) were significantly lower than those used to preserve foods and with antimicrobial activity. 12,17 It is, therefore, possible that the mitochondrial damage by p-cymene, shown in the present study, ie, proton leak through the Fo fraction of mitochondrial ATP synthase with perturbations in mitochondrial proton motive force and loss of ATP synthesis capacity required to support the regulation of several cell functions, might occur in humans and perhaps contribute to some side effects. isolation of rat liver mitochondria Mitochondria were prepared from Wistar rats (250-300 g) by differential centrifugation as described elsewhere. 22,23 Rats were euthanized by decapitation and liver was removed, finely minced, and washed in ice-cold isolation medium containing 250 mM sucrose, 0.5 mM EGTA, 0.5 mM EDTA, 10 mM HEPES, pH 7.4, and 0.1% (w/v) fatty acid-free bovine serum albumin (BSA). 24 Tissue fragments were quickly homogenized with a motor-driven Teflon Potter homogenizer in the presence of ice-cold isolation medium. Liver homogenate was centrifuged at 800g for 10 minute (IEC B-20A centrifuge) at 4°C and mitochondria recovered from the supernatant by centrifugation at 10,000g for 10 minutes. The mitochondrial pellet was resuspended in washing medium using a paintbrush and centrifuged twice at 10,000g for 10 minute before obtaining a final mitochondrial suspension. EGTA and defatted BSA were omitted from the final washing medium, which was adjusted to pH 7.2. 25 Mitochondrial protein content was determined by the Biuret method 26 using BSA as the protein standard.

Mitochondrial respiration
Mitochondrial respiration was measured polarographically using a Clark-type oxygen electrode (Yellow Springs Instruments, OH) 27 inserted into a 1 mL water-jacketed sealed glass chamber with constant magnetic stirring maintained at 30°C, and connected to a suitable recorder. Mitochondria (1 mg protein/mL) were suspended in a standard respiratory medium containing 130 mM sucrose, 50 mM KCl, 5 mM MgCl 2 , 0.1 mM EGTA, 5 mM KH 2 PO 4 , and 5 mM HEPES, pH 7.4. The initial rate of oxygen consumption (state 2 respiration) was recorded following addition of glutamate plus malate (5 mM/2.5 mM), and state 3 rate of respiration was induced by the subsequent addition of 200 nmol of ADP. After a measurable state 4 rate respiration (ie, the rate after ADP is phosphorylated), a second pulse of ADP was added but the phosphorylative cycle was inhibited by adding 1 µg of oligomycin (Olig) (st4-olig). p-Cymene (in DMSO solution, up to 0.2% of the experiment's final volume) and ATP (100 nmol/mg protein) were incubated with mitochondria for 3 minutes prior to the addition of substrate. Control experiments were performed in the presence of 0.2% DMSO. FCCP-stimulated respiration was induced with the addition of 1 µM FCCP added 2 minutes after mitochondria energization. Respiratory control ratios (RCR = state 3/state 4 respiration), respiratory states, and ADP/O (the ratio between the amount of ADP added and the O 2 consumed during state 3 respiration) were determined according to Chance and Williams. 28

Mitochondrial membrane potential determination
The mitochondrial membrane potential (∆Ψ) was monitored by evaluating transmembrane distribution of the lipophilic cation tetraphenylphosphonium (TPP + ) with a TPP + -selective electrode prepared according to Kamo et al 29 using a Ag/ AgCl 2 -saturated electrode as reference (model MI 402; Microelectrodes, Inc, Bedford, NH). TPP + uptake was measured from the decreased TPP + concentration in the medium sensed by the electrode. The potential difference between the selective electrode and the reference electrode was measured with an electrometer and recorded continuously in a Linear 1200 recorder. The voltage response of the TPP + electrode to log [TPP + ] was linear with a slope of 59 ± 1, in good agreement with the Nernst equation. Reactions were carried out in a chamber with magnetic stirring in 1 mL of the standard medium (130 mM sucrose, 50 mM KCl, 2.5 mM MgCl 2 , 2.5 mM KH 2 PO 4 , 100 µM EGTA, and 5 mM Hepes, pH 7.4) supplemented with 3 µM TPP + . 27 A matrix volume of 1.1 µL/mg protein was assumed. No correction was made for the "passive" binding contribution of TPP + to the mitochondrial membranes because the purpose of the experiments was to show relative changes in potentials rather than absolute values. As a consequence, we can anticipate a slight overestimation of ∆Ψ values.
Mitochondria (1 mg/mL) in the standard respiratory medium were energized by the addition of 5 mM glutamate/2.5 mM malate. After a steady-state distribution of TPP + had been reached (ca. 1 minute of recording) ADP was added and ∆Ψ fluctuations recorded. p-Cymene and ATP (100 nmol/mg protein) were preincubated for 3 minutes prior to mitochondria energization.

Measurement of mitochondrial swelling
Mitochondrial osmotic volume changes were followed by monitoring the decrease in absorbance (light-scattering) at 540 nm with a Perkin Elmer, Lambda 6 UV/VIS spectrophotometer. 23 The reactions were performed with 1 mg of mitochondrial protein in 2 mL of a reaction medium containing 200 mM sucrose, 10 mM Tris-Mops (pH 7.4), 1 mM KH 2 PO 4 and 10 µM EGTA. p-Cymene was added to mitochondrial suspensions in the absence and presence of respiratory substrates and preincubated for 3 minutes at 30°C.

Evaluation of mitochondrial permeability transition pore
Mitochondrial Ca 2+ fluxes were measured by monitoring the changes in Ca 2+ concentration in the reaction medium using the hexapotassium salt of the fluorescence probe Calcium Green 5-N. 30 Mitochondria (0.5 mg) were suspended in 2 mL of reaction medium containing 200 mM sucrose, 10 mM Tris-Mops, 1 mM KH 2 PO 4 , 10 µM EGTA, 2 µM rotenone, and 0.5 µg oligomycin/ml, pH 7.4, supplemented with 100 nM of Calcium Green 5-N. Fluorescence was recorded continuously in a water-jacketed cuvette holder at 30°C using a Perkin-Elmer spectrofluorometer LS-50 B with an excitation wavelength of 506 nm (slit 4 nm) and an emission wavelength of 532 nm (slit 6 nm). 25 Opening of mitochondrial permeability transition pore (MPT) was induced by addition of Ca 2+ (60 nmol/mg protein) and energization with succinate (5 mM) and prevented by preincubation with cyclosporin A (CsA) (0.85 µM). p-Cymene (25 nmol/mg protein) was incubated with mitochondria for 3 minutes before Ca 2+ addition and energization with succinate.

statistical data analysis
The results were expressed as the mean ± SD of 4 of 6 independent experiments and the data were analyzed using oneway ANOVA followed by the Tukey post-test, for multiple comparisons. A P value ,0.05 was considered statistically significant.

Results
This study evaluated the effects of p-cymene in the mitochondrial respiratory chain and in the phosphorylation efficiency of rat liver mitochondria. The energization of mitochondria with glutamate/malate in the absence of p-cymene induced a slight increase in the oxygen consumption (state 2 respiration) ( Figure 2A) due to the activation of the respiratory chain. The preincubation of mitochondria with 25, 50, 75 and 100 nmol p-cymene/mg of protein did not induce a significant increase in state 2 respiration compared with mitochondria incubated in the absence of p-cymene (Control) (Figure 2A).
The addition of ADP to mitochondria induced strong oxygen consumption (56.6 nmol O 2 /min/mg of protein) (state 3 respiration) ( Figure 2B, Control) in contrast to that observed in state 2 respiration (8.4 nmol O 2 /min/mg of protein) ( Figure 2B, Control). The preincubation of mitochondria with 25 and 50 nmol p-cymene/mg of protein did not decrease significantly the rate of ADP phosphorylation (state 3 respiration) ( Figure 2B). However, state 3 respiration was significantly decreased when mitochondria were incubated with p-cymene at concentrations .75 nmol/mg of protein ( Figure 2B), suggesting that p-cymene affects the phosphorylation capacity of rat liver mitochondria.
When all ADP was phosphorylated, mitochondria returned to state 4 respiration (8.4 nmol O 2 /min/mg of protein) ( Figure 2C) where the oxygen consumption is lower than in state 3 ( Figure 2B) and similar to the observed in state 2 respiration (8.4 nmol O 2 /min/mg of protein) (Figure 2A).
The preincubation of 25 nmol p-cymene/mg of protein did not induce significant alterations in state 4 respiration. However, p-cymene at concentrations .50 nmol/mg of protein added in vitro to rat liver mitochondria induced a significant stimulation in state 4 respiration ( Figure 2C), suggesting that p-cymene induces a proton leak through the components of the mitochondrial inner membrane.
To evaluate the depressive effect on phosphorylation capacity of liver mitochondria observed in presence of p-cymene uncoupling effects on mitochondrial respiration p-cymene, the RCR (state 3/state 4) and ADP/O indices were determined. As seen in Figure 3A, p-cymene concentrations $25 nmol/mg of protein significantly decreased the RCR compared with control mitochondria. This effect of p-cymene occurred essentially as a consequence of the inhibition of state 3 and stimulation of state 4 respiration. Although we observed a decrease in respiratory state 3, the ADP/O ratio of mitochondria incubated with p-cymene was not statistically different compared with the control ( Figure 3B). The stimulation of state 4 respiration induced by p-cymene could result from a membrane proton permeabilization as a consequence of either mitochondrial membrane disruption or proton shuttling. In order to clarify this mechanism, the effects of p-cymene on the integrity of the mitochondrial membrane were evaluated by monitoring the decrease in absorbance (light scattering) at 540 nm ( Figure 4). The addition of Triton X-100, which induces disruption of the cellular membrane integrity, strongly decreased the light-scattering of mitochondrial suspension reflecting an extensive swelling of mitochondria ( Figure 4). The preincubation of mitochondria with p-cymene at concentrations that stimulate state 4 respiration, 75 and 150 nmol p-cymene/mg of mitochondrial protein, did not significantly decrease the light-scattering of non-energized mitochondria compared with the effects of Triton X-100, suggesting that p-cymene in the concentrations studied did not induce mitochondrial membrane disruption ( Figure 4).
Although we did not observe a statistically significant stimulation of respiratory state 2 induced by p-cymene on mitochondria energized with glutamate/malate (before ADP addition) (Figure 2A), respiratory state 4 (after ADP phosphorylation) was stimulated by this compound ( Figure 2C). When we evaluated the effect of p-cymene on state 2 respiration in the presence of ATP, it was observed that the addition of p-cymene at concentrations ,50 nmol/mg of protein did not induce a significant increase in state 2 respiration ( Figure 5A). However, mitochondria co-incubated with ATP in the presence of 75 and 100 nmol p-cymene/mg of protein stimulated state 2 respiration compared with control, ie, in the presence of ATP alone ( Figure 5A). These results are similar to those observed in state 4 respiration ( Figure 2C), suggesting an increased stimulatory effect in the presence of exogenous ATP or synthesized by the phosphorylation system.
To clarify the different effects of ATP on p-cymeneinduced respiratory changes, further experiments were performed with oligomycin, an inhibitor of proton translocation through the Fo fraction of ATP synthase ( Figure 5B). The addition of oligomycin to mitochondria preincubated with 25 nmol p-cymene/mg of protein did not induce a significant decrease in state 4 respiration (P . 0.05). At concentrations .50 nmol p-cymene/mg of protein, the addition  of oligomycin to mitochondria during respiratory state 4 decreased O 2 consumption stimulated by p-cymene as a function of concentration ( Figure 5B). These results suggest that p-cymene in the presence of ATP induces H + leak through the Fo fraction of complex V of the respiratory chain. ∆Ψ is fundamental for the phenomenon of oxidative phosphorylation, the conversion of ADP to ATP via ATP synthase. The mitochondrial respiratory chain pumps H + out of the mitochondrial matrix across the inner mitochondrial membrane. The H + gradient originates an electrochemical potential (∆p) resulting in a pH (∆pH) and a voltage gradient (∆Ψ) across the mitochondrial inner membrane. Dissipation of ∆p by permeabilization of membrane to H + impairs oxidative phosphorylation efficiency of mitochondria.
∆Ψ developed by mitochondria after energization with glutamate/malate was ≈−223 mV (negative inside) ( Table 1). p-cymene did not induced significant alteration in ∆Ψ and ADP phosphorylative cycle at concentrations ,50 nmol/mg protein. However, we observed that at concentrations above 75 nmol/mg protein the depolarization induced by ADP and the lag phase (time necessary for ADP phosphorylation) were significantly changed and the effects were potentiated in the presence of ATP (Table 1).
When MPT was induced by Ca 2+ in the presence of inorganic phosphate, we observed that isolated mitochondria incubated with 40 nmol Ca 2+ /mg protein rapidly accumulated and retained the Ca 2+ from the medium (Figure 6). In contrast, when mitochondria were incubated with 60 nmol Ca 2+ /mg protein they accumulated Ca 2+ from the medium but after some time they released the accumulated Ca 2+ , this effect being prevented by CsA addition. The presence of p-cymene did not significantly affect the effect of Ca 2+ in any of the conditions tested ( Figure 6).

Discussion
According to previous studies, the antimicrobial effect of monoterpenes such as thymol, carvacrol, p-cymene, and γ-terpinene may result, partially at least, from a gross perturbation of the lipid fraction of the plasmic membrane of the microorganism. 13,31 In addition to being related to the physicochemical characteristics of the compounds (such as lipophilicity and water solubility), this effect seems to be dependent on the lipid composition and net surface charge of membranes. 7 Moreover, it has been reported that these monoterpenes decreased the intracellular ATP pool in different microorganisms and increased the extracellular ATP, also indicating a disruptive action on the cytoplasmic membrane. 32 However, this unspecific mechanism involved in the antimicrobial action of these monoterpenes, also used to preserve and give specific flavors to foods, could contribute to cytotoxic effects on mammalian cells and the putative side effects remain poorly characterized. Mitochondria serve as sensors of the xenobiotic-induced toxicity that ultimately leads to cellular disassembly. 20 In this work, we report the effects of p-cymene, a thymol and carvacrol precursor, on rat liver mitochondria to clarify its effects on bioenergetic functions and membrane integrity of mitochondria. It was observed that p-cymene did not affect state 2 respiration induced by glutamate/malate, but decreased state 3 and stimulated state 4 respiration (Figure 2). The different effect on states 2 and 4 of respiration suggest that p-cymene effects depend on the energetic charge, ie, the ATP levels, and that p-cymene induces damage in the mitochondrial membrane permeability. This permeabilization of membrane to protons, as indicated by the stimulation of state 4, is not a consequence of mitochondrial membrane disruption or change in lipid-protein interactions, since this monoterpene induced only a slight swelling of non-energized mitochondria ( Figure 4). Supporting this assumption is the observation that the stimulation of state 4 was inhibited by oligomycin (Figure 5A), an inhibitor of proton translocation through the Fo fraction of ATP synthase, indicating that p-cymene induced membrane permeabilization to protons by affecting the conformational structure of Complex V proteins, namely the Fo fraction of ATP synthase. Based on this observation and the absence of any significant change in mitochondrial volume (Figure 4), we may propose that the cell toxicity of p-cymene could not be related to disruption of the cell membrane but to the changes in cellular ATP levels.
The possible mechanisms of inhibition of energy regeneration capacity are related to the decrease in ∆ψ and ADP phosphorylation rate also inferred from the decrease in state 3 respiration ( Figure 2B), increasing the time required to ATP synthesis (Table 1) and promoting a rapid decline in cellular ATP pools. Moreover, RCR (state 3/state 4) ( Figure 3A) ratio was also decreased due to the inhibition of state 3 and stimulation of state 4 respiration, indicating an uncoupling effect of p-cymene on the oxidative phosphorylation system. Although not statistically significant but in agreement with these observations, the changes in the ADP/O ratio ( Figure 3B) indicate a decrease in the phosphorylative capacity rate of mitochondria induced by p-cymene according to the increase in the lag phase ( Table 1). The proton leak through the Fo fraction of mitochondrial ATP synthase promoted by p-cymene ( Figure 5B) explains the increase in state 4 respiratory rate, collapse of ∆Ψ, and consequent uncoupling effect on the phosphorylation system, with perturbations in mitochondrial proton motive force and loss of ATP synthesis capacity required to support the regulation of several cell functions (Table 1). Therefore, although it is common for reviewers of spice oils to ascribe their cytotoxic effects to