{'fraction_non_alphanumeric': 0.05532617671345995, 'fraction_numerical': 0.06358381502890173, 'mean_word_length': 4.970443349753695, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Differential Responses of Male and Female Rats: Composition and Accumulation of Essential Fatty Acids in Plasma

This study explores gender differences in fatty acid composition and metabolism in Long Evans adult rats that were orally administrated a mixture of d5‐18:2n‐6 and d5‐18:3n‐3 at dosages of 12 and 60 mg/kg BW, respectively, after over 8 weeks of a controlled diet containing adequate levels of linoleic (LA) and alpha‐linolenic (LNA) acids. Caudal vein blood was drawn at 2, 4, 8, 12, 24, 48, 96 and 168 h after administration of the stable isotopes. The unlabeled endogenous fatty acids and stable isotope labeled fatty acids in plasma were quantitated by GC/FID and GC/NCI/MS, respectively. In female rats, a greater percentage of plasma 22:5n‐6, 22:6n‐3 and a lower percentage of 20:4n‐6 was observed relative to males. On a per dosage basis, female rats accumulated more d5‐22:4n‐6, d5‐22:5n‐6, d5‐18:3n‐3, d5‐20:5n‐3 and d5‐22:5n‐3 than male rats (P<0.05). In summary, female rats have a tendency to accumulate a higher concentration of 22:5n‐6 and 22:6n‐3 in the plasma compartment than do the male rats and this is reflected in the in vivo fatty acid metabolism of stable isotopic precursors.