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The inhibitory effect of porphyromonas gingivalis (P. gingivalis) on Astragali radis extract

The purpose of this study was to evaluate the inhibitory effect of Astragali radis extract in porphyromonas gingivalis (P. gingivalis). This study used Astragali radis leaves cultivated in Kangwon-do after adding 70% ethanol fivefold. The Astragali radis were then concentrated (Gotary vacuum evaporator; N-Nseries, EYELA Co., Japan) and placed under an aspirator (A-3S, EYELA Co., Japan) and a freeze dryer (Ilshin Lab Co., South Korea). The freeze-dried sample was dissolved into dimethlysulfoxide (DMSO). The inhibitory effect of Astragali radis extract was investigated using measurement of the clear zone and the growth inhibitory effect. The measurement of the clear paper disc circle using a growth inhibitory section showed P. gingivalis inhibition with 11 mm at 20 mg/mL. The survival rate of P. gingivalis calculated from the colony-forming units (CFUs) showed growth inhibition as the concentration increased. However, the inhibitory effect of 5 mg/mL, 10 mg/mL and 20 mg/mL did not show a significant antimicrobial effect, although an inhibition of bacterial growth was observed at 20 mg/mL. Based on the results of this study, Astragali radis extract inhibited the growth of P. gingivalis from a 5 mg/mL concentration.