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On the concentration of radioimmunoassayable FSH circulating in blood throughout human pregnancy.

THE PERIODIC renascence of interest in follicle stimulating activity (FSA) of human chorionic gonadotrophin preparations has recently recurred in consequence to A) recent immunobiologic evaluations of the FSA of HCG (1), and B) a report of relatively high levels of radioimmunoassayable FSH, without variation, in the serum of women throughout pregnancy (2). However, in contrast to the latter reports of Faiman, Ryan et al. (2), Jaffe, Lee and Midgley (3) observed only low levels of radioimmunoassayable serum FSH at the time of inception of pregnancy. Because of the marked discrepancy in results obtained by these 2 groups, each utilizing a different but “specific” radioimmunoassay for human FSH, we have re-evaluated serum FSH levels at the inception of and throughout the entire course of pregnancy, by means of our own radioimmunoassay for human FSH (4). Details of the radioimmunoassay techniques for measurement of LH-HCG and FSH utilized in the present study, and their application to the estimation of serum ...