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Impact of Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) on a heterologous tsetse fly host, Glossina fuscipes fuscipes

Background Tsetse flies (Diptera: Glossinidae) are the vectors of African trypanosomosis, the causal agent of sleeping sickness in humans and nagana in animals. Glossina fuscipes fuscipes is one of the most important tsetse vectors of sleeping sickness, particularly in Central Africa. Due to the development of resistance of the trypanosomes to the commonly used trypanocidal drugs and the lack of effective vaccines, vector control approaches remain the most effective strategies for sustainable management of those diseases. The Sterile Insect Technique (SIT) is an effective, environment-friendly method for the management of tsetse flies in the context of area-wide integrated pest management programs (AW-IPM). This technique relies on the mass-production of the target insect, its sterilization with ionizing radiation and the release of sterile males in the target area where they will mate with wild females and induce sterility in the native population. It has been shown that Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infection causes a decrease in fecundity and fertility hampering the maintenance of colonies of the tsetse fly G. pallidipes. This virus has also been detected in different species of tsetse files. In this study, we evaluated the impact of GpSGHV on the performance of a colony of the heterologous host G. f. fuscipes, including the flies’ productivity, mortality, survival, flight propensity and mating ability and insemination rates. Results Even though GpSGHV infection did not induce SGH symptoms, it significantly reduced all examined parameters, except adult flight propensity and insemination rate. Conclusion These results emphasize the important role of GpSGHV management strategy in the maintenance of G. f. fuscipes colonies and the urgent need to implement measures to avoid virus infection, to ensure the optimal mass production of this tsetse species for use in AW-IPM programs with an SIT component.


Background
Tsetse flies (Diptera: Glossinidae) are the only cyclical vectors of the pathogenic African trypanosomes that cause human African trypanosomosis (HAT) or sleeping sickness and African animal trypanosomosis (AAT) or nagana in sub-Saharan Africa [1]. There are 33 species and subspecies of tsetse flies that all belong to the genus Glossina, divided into the Morsitans, Fusca, and Palpalis groups [2]. Although all tsetse species can transfer pathogenic trypanosomes, members of the Palpalis and Morsitans groups are the primary trypanosome vectors [3]. For instance, G. f. fuscipes is a significant vector of trypanosomes in central Africa [4], particularly in Uganda and Western Kenya [5]. In the absence of effective vaccines and drugs against HAT and AAT [6] vector control represents the most efficient strategy to manage these diseases in mainly rural areas [7,8]. Currently accepted tsetse control tactics are the sequential aerosol technique [9], stationary bait methods (traps and targets) [10], the live bait technology [11] and the sterile insect technique (SIT). The SIT is based on the mass-rearing and sterilization of males with ionizing radiation (e.g., gamma irradiation), and the sequential release of adequate numbers of sterile male insects in the target area [12]. Mating between sterile males and wild females will result in non-viable embryos, leading to the gradual reduction of the target insect population [13]. The SIT has proven to be a powerful control tactic for use against tsetse flies and other Diptera as part of area-wide integrated pest management (AW-IPM) approaches [14].
The implementation of AW-IPM programs with an SIT component against tsetse flies poses significant challenges with respect to colonization and mass-rearing of the target species. Many factors, such as infections with pathogens when the insects are reared continuously or under suboptimal rearing conditions [15], might lead to failures in establishing and maintaining large tsetse colonies and, as a consequence, fail to produce insects of adequate quality.
Infections of tsetse flies derived from natural populations and laboratory colonies with the pathogenic salivary gland hypertrophy virus (SGHV) [16][17][18], a member of the Glossina Hytrosavirus genus and Hytrosaviridae family have been frequently observed [19]. SGHV is a rod-shaped enveloped virus (100 × 700-1000 nm) containing a large double-stranded DNA genome of 190 kb [19]. The virus infection is mostly asymptomatic in tsetse flies, but in some cases it can lead to the development of salivary gland hypertrophy (SGH) symptoms, which has been associated with a reduction in the flies' productivity and eventually loss of the colony [20][21][22]. SGH prevalence of this virus in natural tsetse populations vary across tsetse species and their locations, but are usually low (prevalence of 0.3 to 7%) [23] However, under mass-rearing conditions of Glossina pallidipes, high prevalence rates have been observed that were associated with the use of the in vitro membrane feeding technique that favors horizontal transmission of the virus. In G. pallidipes, a species that is considered an efficient vector of trypanosomes [24], SGH symptoms were associated with abnormalities of the ovaries and testicular degeneration, leading to reduced productivity in both male and female flies [15,23,25,26]. Data available on prevalence rates of the virus in colonies of G. pallidipes showed that colony decline and eventual collapse could not be averted when the SGH infection rate in the colony reached 70% (Abd-Alla et al., 2016). To mitigate the negative effects of the virus on colony performance, several virus management strategies were developed that have proven to be effective [27][28][29].
Although SGH symptoms have been detected in natural populations of other tsetse species such as Glossina austeni, G. morsitans morsitans, G. nigrofusca nigrofusca and G. pallicera pallicera [16,30,31], no SGH symptoms have been observed in G. f. fuscipes but asymptomatic infection was detected [32]. However, in laboratory colony, intrahemocoelic injections of GpSGHV into five heterologous tsetse species (G. brevipalpis, G. m. morsitans, G. m. centralis, G. f. fuscipes and G. p. gambiensis) showed a significant increase in the titer of viral DNA, demonstrating the ability to replicate in these heterologous species [33].
The Government of Ethiopia has embarked on an AW-IPM program with an SIT component to eradicate a G. f. fuscipes population in the Deme river valley of Southern Ethiopia [34][35][36]. The campaign required the establishment and expansion of a colony of the target species in the mass-rearing facility in Kality on the outskirts of Addis Ababa. The colony was initiated with seed material from a colony maintained at the Slovak Academy of Sciences (SAS), Slovakia. Although colony growth was acceptable in the initial stages subsequent low productivity and high mortality resulted in a drastic reduction in colony size. Similar observations were made at the SAS, where the colony was lost. It is worth noting that more than one tsetse species is being maintained in both facilities, including G. pallidipes, a species known to be harbor GpSGHV, a situation that may facilitate the transmission of GpSGHV from one tsetse species to another especially if both species were fed using the same membrane as was the case in the SAS colonies. It should be noted that SGHV was detected by PCR in natural populations of G. f. fuscipes with a prevalence of 25-40% [32] and an increase in virus titer in GpSGHV injected flies has recently been demonstrated [33].
This study was undertaken as part of efforts to understand the possible causes of the poor colony performance. In this study, we report on the impact of GpSGHV on the performance of G. f. fuscipes flies using standard quality control parameters, such as adult longevity, female productivity and mortality, flight propensity, mating ability, and insemination rate.

Tsetse flies
The G. f. fuscipes flies used in this study originated from a colony that was established from wild collected material in the Central African Republic (CAR) and maintained since 1986 at the Insect Pest Control Laboratory (IPCL) of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Seibersdorf, Austria. Experimental flies were fed for 15-20 min, three times per week with defibrinated bovine blood using an artificial (in vitro) membrane feeding system [37]. The adult flies were held in medium size cages (11 cm diameter × 5 cm high) at a ratio of 1:3 male to female under standard tsetse colony rearing conditions (24 ± 0.5°C and 75 ± 5% relative humidity (RH)) [38]. The SGHV is not detectable in this colony by PCR.

Preparation of virus inoculum and intra-hemocoelic injection
The GpSGHV inoculum was prepared from intact hypertrophied salivary glands dissected from a 10-dayold male G. pallidipes showing overt SGH symptoms [39]. Briefly, the hypertrophied glands were homogenized in phosphate buffered saline (PBS) at a concentration of one pair of glands/ml and the homogenate was centrifuged at 400 x g for 2 min at room temperature. The supernatant was transferred to a new sterile tube and used immediately after preparation of the inoculum.
Using a 1 ml Myjector U-40 Insulin type syringe (Teruma, Leuven, Belgium) either 2 μl of filter-sterilized PBS (control) or the virus suspension was injected into the thoracic cavity of prechilled adult flies. For each treatment, newly emerged teneral (male and female) flies were injected and placed into standard holding cages (20 cm diameter × 5 cm high) at the required mating ratio and each experiment was replicated 3 times. Non-injected and PBS-injected flies were used as non-injected controls to evaluate the impact of injection and the virus infection on the flies' performance.

Prevalence of GpSGHV infection in G. f. fuscipes injected flies
The tsetse genomic DNA was extracted from individual non-injected, PBS-and virus-injected flies using the DNeasy Blood & Tissue kit (QIAGEN Inc., Valencia, CA) following the manufacturer's instructions. The titer of GpSGHV was determined in G. f. fuscipes injected males and females on 0, 9 and 18 days post injection by polymerase chain reaction (PCR) using the method previously described by Abd-Alla et al. [20]. Equal volume of individual DNA sample was pooled (n = 6 for females and n = 2 for males) and measured to determine the DNA concentration by spectrophotometry (Nanodrop-Synergy H1 Multi-Mode Reader, BioTek, Instruments, Inc., USA), DNA samples were diluted to final concentration of 4 ng/μl and 5 μl was used as template for the qPCR reaction. The qPCR was performed with odv-e66 (GpSGHV ORF5) gene using the method previously described [20,39,40] and the tsetse β-tubulin gene was used as a housekeeping gene to normalize the qPCR reactions.

Impact of GpSGHV infection on survival and productivity of G. f. fuscipes
To evaluate the impact of GpSGHV challenge on G. f. fuscipes, their productivity and longevity under both normal feeding (blood meal offered three times per week) and starvation stress (no blood feeding) conditions was monitored in non-, PBS-and virus-injected flies. For each treatment, seven males and twenty-one females were kept in standard holding cages and each treatment was replicated 3 times. The productivity data is presented as total pupae over the experimental period per initial female (PPIF).

Impact of GpSGHV infection on the flight propensity of G. f. fuscipes
The flight propensity of virus injected flies, non-injected and PBS-injected flies (the latter two as negative controls) was assessed at 7, 14, 21, 28, 35 and 42 days post injection under normal feeding conditions. Flight tests were carried out in netted cubic mating cages (45 × 45 × 45 cm) that contain a black Polyvinyl Chloride (PVC) tube (8.9 cm diameter, 3 mm thick wall, 10 cm high). The PVC tube allowed light entering only from the top and the walls were coated with unscented talcum powder to prevent the flies from walking out the tube [41]. Standard FAO/IAEA/ USDA protocols (FAO/IAEA/USDA, 2014 http://www-naweb.iaea.org/nafa/ipc/public/QualityControl.pdf) were used with a few modifications i.e. rather than using pupae, the adult flies were chilled at 4°C for 5 min prior to the test, to enable the transfer to the tube. For each test, seven chilled males and twenty-one chilled females were put in a plastic Petri dish (90 mm diameter) with the base covered by black porous paper, and the number of flies that had escaped from the tube "flier" was recorded during one hour [42]. The light intensity at the top of the tubes was 500 lx. Six replicates were conducted for each treatment.
Impact of GpSGHV infection on the mating ability and insemination rate of G. f. fuscipes The mating ability and the insemination rate of untreated (normal colony) G. f. fuscipes males of different ages (3-, 6-, 9-and 12-days post emergence) were assessed to determine the optimal mating age [43]. Forty (40) teneral males were released in mating cages mating cages (45 × 45 × 45 cm), followed 15 min later by an equal number of 9 -day old virgin females for mating. Mating events were observed under standard tsetse rearing conditions from 9:30 to 12:30 h to cover the morning mating activity peak [44]. The optimal mating age test was replicated 3 times and mating tests of virus-challenged flies were repeated 9 times. All flies were offered a blood meal 24 h before mating to increase the mating rate, and non-fed flies were removed and replaced. The propensity for mating ratio was calculated according to the proportion of females that mated for each treatment [44]. After determining the optimal male mating age, 6 to 9-day old non-injected, PBS-injected, and virus-injected virgin males (40 males) were tested as described above to determine mating ability and insemination rate of experimental flies.
Mating pairs were transferred to small cages (4 cm diameter × 6 cm high) and kept for 24 h, after which the males were removed and the females dissected under a binocular microscope to determine insemination rate. Mated female flies were dissected in PBS under a binocular microscope and the insemination rate and spermathecal contents were assessed subjectively at × 100 magnification using a Carl Zeiss compound microscope [45]. The spermathecal fill and insemination rate were obtained by assessing the content of the spermathecae pairs. Spermathecal fill was scored to the nearest quarter for each spermathecae separately as empty (0), quarter full (0.25), half-full (0.50), three-quarter-full (0.75) and full (1.0), For the statistical analysis, quarter full (0.25), half-full (0.50), three-quarter-full (0.75) were considered as partial fill. The amount of sperm transferred was then computed as the mean spermathecal filling values of the spermathecae pairs [46].

Statistical analysis
The significance of the virus injections on the various parameters was assessed by an ANOVA test [47]. Pairwise comparisons between group means (PBS vs. virus injections, non-injected vs. virus injections and non-injected vs. PBS injections effect on flies) was than determined by Tukey's honestly significant difference (HSD) test. The analyses were performed in R [48,49] using RStudio version 3.4.1. [50] The data was transformed using the Box-Cox procedure from the packages. ggplot2 [51], lattice v0.20-35 [52] and MASS v7.3. [53].
All survival analyses were performed using Graph Pad Prism version 5.0 for Windows (GraphPad Software, San Diego California, USA; graphpad.com). The effect of the treatments on fly longevity was analysed using a Log-rank (Mantel-Cox) test. Differences between treatments pairs were tested using the Bonferroni method. Mean longevity (or age in days at death) was calculated from the sum of the number of live flies on each day until the death of the last fly, divided by the number of flies in the group at the start of the experiment. The level of significance was 0.05 for all statistical analyses.

Detection of GpSGHV infection in injected flies
The GpSGHV titer in virus-injected flies was assessed by qPCR at various times post injection to investigate whether the virus could infect and replicate in injected flies. The qPCR results indicate a significant increase in the virus titer over time (F = 1.34, df = 1, 34, P < 0.001).
The results indicate that the virus replication was rather slow as no significant increase in the virus titer between 0 time and 9 dpi was observed, but later the virus titer increase by 5. 22 fold change at 18 dpi (Fig. 1). In addition, results demonstrated a significant difference in the virus titer between the virus injected flies and negative controls (PBS-injected flies (F = 21.51, df = 1, 68, P < 0.001).

Impact of GpSGHV infection on G. f. fuscipes productivity and survival
Virus challenge reduced the productivity of the flies significantly (F = 52.05, df = 2,6, P < 0.0001) (Fig. 2). This reduction was significant when compared with PBSinjected (P < 0.001) and non-injected (P < 0.0005) flies. The injection process had no impact on their productivity as no significant difference (P = 0.079) was observed between non-injected and PBS-injected flies.
Under starvation stress, the survival rate of the flies was significantly lower than the survival under normal condition regardless of treatment. However, the virusand PBS-injected females showed a lower survival (Log-rank X 2 = 87.02, df = 2, P < 0.001, less than 10 days) as compared with the non-injected females (Fig. 3b). Similar to female flies, the virus-and PBS-injected males lived a significantly shorter time (Log-rank X 2 = 8.741; df = 2, P < 0.001) (less than 10 days, similar to female survival) as compared with the non-injected males (Fig. 3d).

Flight propensity of GpSGHV injected G. f. fuscipes
GpSGHV infection had no significant impact (F = 1.4; df = 2, 42; P = 0.25) on the flight propensity of G. f. fuscipes males and females as compared with the PBS-injected and non-injected flies (Fig. 4). The average percentage of fliers for different treatments was evaluated at different times post emergence (7, 14, 21, 28, 25, 42 days). No significant difference in flight propensity was recorded at different times regardless of treatment (F = 0.08; df = 1, 52; P = 0.91).

Impact of GpSGHV infection on G. f. fuscipes flies mating ability
In order to assess the impact of the GpSGHV infection of the flies' mating ability, it was essential to determine the optimal mating age of untreated flies. Mating propensity of 3, 6, 9 and 12 day-old males differed significantly (F = 3.07, df = 3, 8, P < 0.001) with 3 day-old males having a significantly lower mating success as compared with older males (P < 0.001). However, no significant difference in the mating propensity of 6, 9 and 12 day-old males (P > 0.05) was observed (Fig. 5a). Therefore, 6-9 day-old males were used to assess the impact of GpSGHV infection on the mating ability of male flies. Most of the mating pairs were formed in the first hour after introduction of the females into the mating cages, and mating gradually reduced during the remaining 2 h of the test. In general, mating propensity of non-, PBS-and virus-injection flies was significantly different (F = 4.89, df = 2, 24, P = 0.016). The mating propensity of virus-injected males was significantly reduced as compared with non-injected males (P = 0.014) (Fig. 5b), while, no significant difference was observed between PBS-injected and non-injected males (P = 0. 59) or between the PBS and virus-injected males (P = 0.11).

Impact of GpSGHV infection on insemination rate
Females mated with untreated males of different ages showed variable insemination rates (Fig. 6a). The proportion of females with empty spermathecae decreased as male age increased (F = 17.89, df = 1, 6, P = 0.005). The percentage of females with partially and fully filled spermathecae increased slightly but not significantly (F  (Fig. 6a).
The GpSGHV injection of the males did not affect the insemination rate for empty, partially and fully filled spermathecae values or empty spermathecal values (F = 0.19, df = 2, 24, P = 0.8261) (Fig. 6b).

Discussion
The challenge in establishing large colonies of tsetse flies in mass-rearing facilities for the implementation of the SIT component in AW-IPM programs has always been a strong driver to explore and identify the key factor(s) affecting tsetse biology. The collapse of colonies of G. pallidipes at the IPCL and in Ethiopia prompted a decade of research on the productivity problems in these colonies and its association with  the GpSGHV. As a result, virus management strategies have been developed to mitigate the instability in production of these colonies [20,[27][28][29]. In view of the similarity of low productivity of the G. pallidipes and G. f. fuscipes colonies maintained at the SAS in Bratislava, Slovakia and Addis Ababa, Ethiopia, this study was conducted to investigate whether a potential GpSGHV infection might contribute to the low performance of the G. f. fuscipes colony. Our data indicates that the presence of the virus indeed reduced various important quality parameters such as adult longevity, female productivity and male mating ability and, in addition, increased the mortality rate. Conversely, flight ability and insemination rate of virus-challenged flies was not affected as compared with uninfected ones.
Despite the negative impact of virus challenge on the flies' performance, no SGH symptoms were observed in injected G. f. fuscipes flies and no virus transmission to the F 1 progeny was detected (data not shown). These findings are in agreement with recent data demonstrating that the GpSGHV can replicate in five heterologous tsetse species without inducing SGH or being vertically transmitted to the F 1 offspring [33]. The results also agree with previously reported data on the significant reduction in the lifespan of G. pallidipes challenged with GpSGHV [54]. In addition, similar results were obtained by injecting the house fly Musca domestica salivary gland hypertrophy virus in a heterologous host, Stomoxys calcitrans, where the infection had a negative impact on survival and fecundity of the heterologous host without the development of SGH symptoms [55].
The GpSGHV infection in G. f. fuscipes affected fitness parameters such as increased mortality and reduced fecundity which are the key parameters for colony stability and growth. Moreover, the effects of virus infection impacted females more than males. This is especially relevant for tsetse flies whose fecundity is low [56]. This negative impact on female mortality and productivity under normal colony conditions may explain the problems in maintaining the colonies (both in Slovakia and in Ethiopia) and its ultimate reduction in colony numbers. The lower female survival due to the presence of the virus agrees with previous reports on G. pallidipes, showing that females with apparent viral infection as indicated by their enlarged salivary glands had a significantly shorter lifespan than females with normal salivary glands [57]. Likewise, the longevity of virus-infected G. m.centralis flies was significantly reduced as compared with uninfected control flies [26]. Our observed positive correlation between male age and mating success was in agreement with previously reported that in field cages, males younger than 8 days showed a significant lower mating ability [43]. Our results showed that 3 day-old males were less successful in mating than older males, but no further significant difference was observed between 6-day old or older males. Similar observations were reported with other species, i.e. 3-day old male G. brevipalpis and G. austeni were less successful in mating as compared with older males [58]. In other studies, 6-8 day old-males G. p. gambiensis were used for mating studies [59] and older G. pallidipes males copulated more often than young males [44,60].
The significant reduction of the mating ability of GpSGHV-challenged male G. f. fuscipes flies is an additional negative impact of the presence of the virus. The observed reduction in mating success as measured in small mating cages that mimic well the situation in standard tsetse holding cages, might partly explain the reduction in the females' fecundity as almost half of the females were not inseminated when offered a mating opportunity with virus-injected males. These results are in agreement with previous studies on the mating performance of G. pallidipes in small laboratory cages [57] or in walk-in field cages [61]. Our data are also in agreement with results of Helicoverpa zea males infected with the Hz-V2 virus, that were slower in approaching healthy females for mating as compared with non-infected males [62,63]. This reduction in mating propensity might be a result of reduced flying and searching activity for females or possibly a negative selection by females against infected males [64].
Our data to imply a different outcome when compared with the results of Odindo [65] who reported no significant difference in mating performance between symptomatically infected and asymptomatic G. pallidipes flies. In addition, in contrast to our study, Jura and Davies-Cole (1992) speculated that SGHV-infected, and hence sterile, G. pallidipes males showed increased mating competitiveness and concluded that these males could be used for SIT applications [66]. Although our and the experiments of Odindo [65] and Jura and Davies-Cole [66] were conducted in similar settings (small laboratory cage), the different results are most likely due to the different tsetse species (G. pallidipes versus G. f. fuscipes) populations or strains used in the study. However, no data are so far available on the impact of the virus in males on the potential selection of females for mating partners. Further studies on the presence of the virus and its impact on the biological mechanisms of mating are necessary.
The virus injection has no significant impact on flight propensity and insemination rate of infected flies. The absence of a negative impact on the adult flight propensity (males and females) observed in this study contradicts the finding of Odindo [64] who speculated that the presence of the virus resulted in reduced physical male activity in G. pallidipes. It also contradicts the observation of Burand and Tan [63] who observed that the Hz-1 virus makes the H. zea male lazier and slower to move. The reduction in the mating propensity of virus-infected males might be due to reduced physical male activity. This might indicate that the physical activity required for the flight propensity test is much less than that required for successful mating and therefore the infected males had the propensity to fly but lost the ability to conduct normal mating activity.
The absence of any significant impact of the virus infection on insemination rate might be due to the completion of sperm development during the pupal stage in Glossina species prior to the virus challenge of the adult stage. The results contradict earlier data indicating that virus infected G. pallidipes males with SGH were unable to successfully inseminate females after mating [21,61].
The difference between our current data and these published earlier might be due to a different level of virus infection (virus infected G. f. fuscipes showed no sign of SGH versus G. pallidipes males with SGH indicating a higher density of virus particle per flies (> 10 6 ) and a different tsetse species.

Conclusions
The data presented in this paper directly demonstrates the negative impact of GpSGHV infection on the establishment and maintenance of G. f. fuscipes colonies, which will be crucial for the production of sufficient male flies of adequate biological quality for the application of the SIT programmes. The combination of increased female fly mortality and the reduction in mating propensity of the virus-infected males will shorten the production period and therefore will necessitate an increase in colony size to compensate for the loss in production. Finally, virus-infected males might have a lower competitiveness under field conditions, which will require increased release rates. These combined effects of the presence of the virus in G. f. fuscipes colonies will impose serious challenges to mass-rear and produce sufficient sterile males of adequate biological quality and will make the SIT component more expensive and less competitive with other control tactics [67]. Management strategies to mitigate the negative effects of virus presence that were based on the use of a clean feeding system (each fly receives a clean blood meal) and the mixing of the blood meals with the antiviral drug valacyclovir were recently developed for G. pallidipes colonies. However, the implementation of these strategies has so far been restricted to G. pallidipes colonies where flies showed clear SGH symptoms [27][28][29]. So far, the absence of SGH symptoms in many tsetse species including G. f. fuscipes has excluded the virus-infection as a possible cause for the poor performance of certain colonies and consequently no virus management strategies were implemented. The data presented in this manuscript strongly indicates that colonies that perform poorly should be screened for the presence of the virus with PCR and in confirmed cases, virus management strategies should be implemented even when no SGH symptoms are observed. Special caution is required in those tsetse mass-rearing facilities where G. pallidipes colonies are maintained with colonies of other tsetse species.