{'fraction_non_alphanumeric': 0.02909647779479326, 'fraction_numerical': 0.009188361408882083, 'mean_word_length': 5.782006920415225, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Effect of high glucose on intercellular adherens junction complex in cultured human peritoneal cells in vitro

Objective:To investigate the effect of the high glucose concentration on the intercellular adherens junction complex in the cultured human peritoneal mesothelial cells(HPMC),and clarify the cause of decreased dialysis efficiency during prolonged peritoneal dialysis. Methodology:All test were performed on the human peritoneal mesothelial cell line(HMrSV 5).Three groups were divided.They were ①normal glucose group treated with 5.5 mmol/L D-glucose;②high glucose group treated with 140 mmol/L D-glucose;and ③high osmolality group with 5.5 mmol/L D-glucose and 140 mmol/L mannitol.Cell detachment and viability were observed at the time of 6,12,24,and 48 hours.The mRNA expression of E-Cadherin and β-Catenin was detected by semi quantitative reverse transcription polymerase chain reaction (RT-PCR),and the protein measured by Westernblot and immunofluorescence.High glucose-induced actin disassembly was determined by fluorescence labeling technique. Results:High glucose caused a time-dependent increase in the cell detachment.The mRNA expression and level of intercellular adherens junction protein(E-Cadherin and β-Catenin)were decreased,and the fluorescence expression immunostaining by anti-E-Cadherin and anti-β-Catenin antibodies was weaker and often discontinuous along the cell contour.When HPMC was incubated in high glucose,F-actin bundles appeared less prominent and changed to irregular network.Mannitol had no effect on actin assembly and on the expression of E-Cadherin and β-Catenin at the same osmolality. Conclusion:These findings suggest that the peritoneal mesothelium is damaged by prolonged peritoneal dialysis using high glucose dialysate,and that impairment of the intercellular adherens junction complex of HPMC by high glucose play an important role in cell detachment and a progressive reduction in dialysis efficiency.