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Green synthesis of copper oxide nanoparticles using Cinnamomum malabatrum leaf extract and its antibacterial activity

Development of green nanotechnology has attracted significant attention of researchers towards the eco-friendly green synthesis of nanoparticles. The present study reports a green synthesis of copper oxide nanoparticles (CuO NPs) using the aqueous leaf extract of Cinnamomum malabatrum in appropriate conditions. It is observed that the leaf extract can act as reducing and stabilizing agents to synthesize CuO NPs. The synthesized NPs are characterized using XRD. The XRD pattern clearly suggests the crystalline nature and monoclinic structure of the synthesized composition. The average crystalline size is calculated using Debye-Scherrer relation and it was found to be 17nm. The FT-IR analysis is carried out in order to investigate the biomolecules present in the synthesized CuO NPs. The presence of CuO NPS is confirmed with EDX. The TEM and SEM images reveal the spherical shape of the NPs and the size of the NPs calculated is consistent with XRD results. The antibacterial activity of these NPs is tested against four human pathogenic bacteria both Gram-positive ( S.aureus ) and Gram-negative (E.coli, P.aeruginosa , and P.mirabilis ). The synthesized CuO NPs show significant antibacterial activity against all the four bacteria. The present study reports the synthesis and characterization of CuO NPs using Cinnamomum malabatrum leaf aqueous extract. The obtained NPs were analyzed by X-Ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, Scanning Electron Microscopy (SEM) with Energy Dispersive X-ray (EDX) and Transmission Electron Microscopy (TEM). Further, the antibacterial activity of the synthesized CuO NPs was examined against four human pathogenic bacteria both Gram-positive ( S.aureus ) and Gram- negative ( E.coli, P.aeruginosa and P.mirabilis ) bacteria using Agar well diffusion method.