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[Emergence of the integron-positive multi-drug resistant strain of Acinetobacter baumanii in Russian hospitals].

AIM
To detect the integron-positive strains among nosocomial Acinetobacter spp. and to determine their relationship on the genotype level.


MATERIALS AND METHODS
Amplification by polymerase chain reaction using primers specific to sequences of the class 1 and 2 intergrons on the genomic DNA template followed by restriction fragments length polymorphism analysis as well as RAPD-genotyping of the integron-positive strains were performed.


RESULTS
Fact of spreading of Acinetobacter baumanii strain containing class 1 integron in medical centers was established. The composition of the integron's gene cassettes was analogous to worldwide (including epidemic) types.


CONCLUSION
Molecular genetic analysis of the mobilized structures (integrons) under the standardization of used approaches are suitable for the surveillance for circulation of epidemic strains of nosocomial pathogens.