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Fast Quantification of Recombinant Protein Inclusion Bodies within Intact Cells by FT‐IR Spectroscopy

The accomplishment of the quantification of the recombinant protein content of whole bacterial cells by FT‐IR spectroscopy by application of chemometrics is shown. Recombinant Escherichia coli cells expressing an inclusion body forming fusion protein were dried on a 96‐well silicon plate for the analysis in a high‐throughput FT‐IR spectrometer. Acquired spectra of additionally conventionally quantified samples were used to establish a multivariate calibration. The obtained method was tested by predicting inclusion body contents of samples not used for the multivariate model. Results from FT‐IR spectra coincided well with the data of universalized electrophoresis analysis. Hence FT‐IR spectroscopy could prove as a fast and simple alternative to conventional quantification methods.