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Research on improving reconstruction quality from holograms of marine algae in digital holographic microscopy

In this paper, we improve reconstruction quality from in-line holograms of marine algae by two phase-retrieval-based reconstruction methods. Digital holographic microscopy, with its high-resolution and volume recording capability, has been applied in the area of monitoring marine algae. We designed a digital in-line holographic microscopy laboratory system to observe and analyze marine algae. Due to the “twin-image” problem caused by the loss of phase information in hologram recording, the reconstructed quality from holograms of marine algae reduced significantly. To enhance reconstruction quality, the alternating projections methods and inverse methods are introduced and implemented on the marine algae holograms. Compared with the traditional backpropagation method, both of the above methods have good performance in improving reconstruction quality from in-line marine algae holograms.