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Phagocytosis of heat-killed radiolabelled Candida albicans by human blood monocytes cultured in vitro.

In vivo experiments indicate that blood monocytes differentiate into macrophages after leaving the circulation (2, 3, 4). Monocytes separated from venous blood and attached to glass surfaces undergo a similar differentiation in vitro (8). In this report, the phagocytic function of monocytes at various stages of this differentiation is demonstrated by means of a technique using radiolabelled Candida albicans. With a view to attachment and engulfment as well as to digestion of yeast particles, quantitative changes of the phagocytic function were found during the first 8 days of culturing.