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Determination of plasma acetoacetate and D-beta-hydroxybutyrate in new-born infants by an enzymatic fluorometric micro-method.

A simple accurate method using 0.1 ml of plasma for determination of acetoacetate (AcAc) and D-β-hydroxybutarate (BHB) is described and applied to new-born infants. Umbilical cord BHB paralleled maternal values. BHB and AcAc increased in the first days of life and decreased after intravenous glucose. Capillary and venous values were well correlated.