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Plant Mediated Green Synthesis, Catalytic Activity and
Antibacterial Assay of Silver Nanoparticles

Plant mediated green synthesis of silver nanoparticles (AgNPs) was carried out with the plant extract of Vitex negundo Linn., a simple method without using harmful chemicals. Herein, a root bark extract is used as reducing and capping agent for synthesizing AgNPs from silver nitrate solution. The characterization of AgNPs were obtained by UV-visible spectrophotometer, X-ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy and energy dispersive spectroscopy. The results were confirmed the presence of silver nanoparticles with UV-visible absorption peak at
around 433-443 nm and its crystalline nature by XRD analysis. The FTIR spectrum confirmed that the synthesized AgNPs are stabilized and protected by various functional groups of biomolecules in extract. The SEM morphology showed the triangular or spherical shape of AgNPs with the size in the range of 80-100 nm and EDS confirmed the presence of elemental silver in the sample. The catalytic activity of AgNPs was investigated in the synthesis of 5-substituted 1H-tetrazole. Furthermore, antibacterial profile showed that biopotent AgNPs exhibited moderate to good antibacterial activity.