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Flatten‐peeled: A new approach to freeze‐fracture morphology

A new way to obtain in a replica vast pictures of membranes situated at the same level in the preparation is described. The tissue specimen is flattened during fixation with a given orientation. It can be fractured afterwards, either along the plane of fixation or, after rotation, perpendicular to this plane. The method utilizes standard freeze‐fracture equipment and readily available materials.