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PCR Use of Highly Conserved DNA Regions for Identification of Sinorhizobium meliloti

ABSTRACT A PCR identification method in which four primers that recognize homologous conserved regions in the Sinorhizobium melilotigenome are used was developed and tested. The regions used for identification were the nodbox 4 locus, which is located in one of the symbiotic megaplasmids, and the mucR gene, which is located in the chromosome. The new method was used to establish a collection ofS. meliloti strains from polluted soils.