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Vine Tea (Ampelopsis grossedentata) extract attenuates CCl4 -induced liver injury by restoring gut microbiota dysbiosis in mice.

SCOPE
Vine tea (Ampelopsis grossedentata), a traditional Chinese tea, has displayed various biological activities. We aimed to investigate the effect of Vine Tea (Ampelopsis grossedentata) extract (VTE) on CCl4 -induced acute liver injury (ALI) in mice and to explore the underlying role of gut microbiota during the treatment.


METHODS AND RESULTS
C57BL/6J mice injected with CCl4 were treated with VTE for 6 weeks. By using H&E staining, immunofluorescence staining, RT-PCR, and western blot, it was shown that VTE treatment significantly ameliorated hepatocyte necrosis, alleviated the mRNA levels of Tlr4, Il-6, iNOS, Acc1, and increased the mRNA levels of Ppar-γ and Hmg-coar compared to the CCl4 group. Also, VTE abrogated the decreased mRNA expressions of Zo-1, Occludin, and Mucin1 in colon tissues. Using microbial 16S rDNA sequencing, VTE treatment significantly downregulated the abundances of some harmful intestinal bacteria like Helicobacter and Oscillibacter. In contrast, VTE upregulated the contents of several beneficial bacteria, such as Ruminococcaceae_UCG-014 and Eubacterium_fissicatena_group. Further, VTE failed to improve ALI in the mice with gut microbiota depletion using antibiotic treatment.


CONCLUSIONS
Our studies suggest that VTE exhibited a protective effect against CCl4 -induced ALI in mice by alleviating hepatic inflammation, suppressing intestinal epithelial barrier injury, and restoring gut microbiota dysbiosis. This article is protected by copyright. All rights reserved.