{'fraction_non_alphanumeric': 0.02885796152271797, 'fraction_numerical': 0.02660663119115841, 'mean_word_length': 5.3834095362508165, 'pattern_counts': {'":': 0, '<': 1, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Distribution and genotype frequency of the C1431T and pro12ala polymorphisms of the peroxisome proliferator activator receptor gamma gene in an Iranian population

carbohydrate metabolism (energy balance), adipocyte BACKGROUND: Peroxisome proliferator activator receptor gamma (PPARγ) is a nuclear transcription factor regulating multiple genes involved in cell growth, differentiation, carbohydrate and lipid metabolism and energy production. Several genetic variations in the PPARγ gene have been identified to be associated with diabetes, obesity, dyslipidemia, insulin resistance, metabolic syndrome and coronary artery disease. The present study was designed to explore the distribution of two common single nucleotide polymorphisms of the PPARγ gene (C1431T and Pro12Ala) in an Iranian population. MATERIALS AND METHODS: Genotype frequencies for these two polymorphisms were compared for 160 healthy Iranian individuals with reports from other populations. The Genotyping was performed using real‐time polymerase chain reaction. RESULTS: The genotype distribution of the C1431T PPARγ polymorphism was 0.869 for the CC genotype, 0.119 for the CT genotype and 0.013 for uncommon TT genotype. Allelic frequencies were 0.93 for C and 0.07 for T allele respectively. For the Pro12Ala polymorphism of PPARγ gene, genotypic distributions and allelic frequencies were, 0.813 for CC, 0.181 for CG and 0.06 for GG and 0.903 for C and 0.097 for G respectively. Allelic and genotypic frequencies for both polymorphisms of PPARγ gene were in Hardy‐Weinberg equilibrium. CONCLUSIONS: Iran is a country with an ethnically diverse population and a comparison of allelic and


Introduction
Peroxisome proliferator activator receptor gamma (PPARγ) is a member of the nuclear receptor superfamily and is a ligand-activated transcription factor that regulates genes responsible for some very important biological functions including: Cell growth, differentiation and metabolism and acts as dietary lipid sensor. [1] The PPARγ gene is located on the human chromosome 3p25-24 and consists of nine exons and as a result of alternative splicing and promoter usage produces four isoforms; PPARγ1, PPARγ2, PPARγ3 and PPARγ4.
PPARγ1 is expressed by most tissues. [2] PPARγ2 is predominantly expressed in adipose tissue and regulates adipocyte differentiation. [3] PPARγ3 expression appears to be confined to macrophages, adipose tissue and the colon. [4] Primer extension studies have confirmed that PPARγ4 mRNA is present in adipose tissue suggesting that the PPARγ4 isoform has a role in adipocyte biology. [5] PPARγ plays critical roles in regulating lipid and carbohydrate metabolism (energy balance), adipocyte BACKGROUND: Peroxisome proliferator activator receptor gamma (PPARγ) is a nuclear transcription factor regulating multiple genes involved in cell growth, differentiation, carbohydrate and lipid metabolism and energy production. Several genetic variations in the PPARγ gene have been identified to be associated with diabetes, obesity, dyslipidemia, insulin resistance, metabolic syndrome and coronary artery disease. The present study was designed to explore the distribution of two common single nucleotide polymorphisms of the PPARγ gene (C1431T and Pro12Ala) in an Iranian population. MATERIALS AND METHODS: Genotype frequencies for these two polymorphisms were compared for 160 healthy Iranian individuals with reports from other populations. The Genotyping was performed using real-time polymerase chain reaction.

RESULTS:
The genotype distribution of the C1431T PPARγ polymorphism was 0.869 for the CC genotype, 0.119 for the CT genotype and 0.013 for uncommon TT genotype. Allelic frequencies were 0.93 for C and 0.07 for T allele respectively. For the Pro12Ala polymorphism of PPARγ gene, genotypic distributions and allelic frequencies were, 0.813 for CC, 0.181 for CG and 0.06 for GG and 0.903 for C and 0.097 for G respectively. Allelic and genotypic frequencies for both polymorphisms of PPARγ gene were in Hardy-Weinberg equilibrium. CONCLUSIONS: Iran is a country with an ethnically diverse population and a comparison of allelic and differentiation, proliferation and insulin sensitivity and create a relationship between environmental factors and metabolic processes of the organism. [6] PPARγ also plays roles in macrophages by enhancing foam cell formation and suppressing inflammatory cytokine production. It may also participate in controlling systemic glucose and lipid metabolism in the liver. [7,8] PPARγ may therefore affect processes involved in atherosclerosis. [9] Since PPARγ is a nuclear transcription factor regulating multiple genes involved in energy production, glucose and lipid metabolism it may be a promising candidate gene for several major common diseases including cardiovascular disease, cancer, diabetes, inflammation and polymorphisms in this receptor may influence the pathology of these diseases. The PPARγ gene has been reported to be associated with various metabolic disorders including diabetes, [10] obesity, [11] dyslipidemia, insulin resistance, metabolic syndrome [12] and coronary artery disease (CAD). [13] Several PPARγ2 gene single nucleotide polymorphisms confer some conformational changes in protein structure that may affect transcriptional activity. [14] Among several genetic variants of the PPARγ gene, two polymorphisms Pro12Ala of the exon B (rs1801282) and the C1431T silent substitution (rs3856806) in the 6 th exon are the most frequently occurring SNPs and have been associated with various diseases and extensive studies have been undertaken to assess the effects of these polymorphisms on many aspects of human physiology. The C1431T polymorphism is also known as the C161T or His477His was identified in 1998 by Meirhaeghe et al., [15] and has been studied in relation to bone metabolism, metabolic syndrome, CAD, obesity and glucose intolerance. The Pro12Ala protein polymorphism is due to a CCA-to-GCA missense mutation and was identified by Yen et al. [16] It is associated with type-2 diabetes mellitus, insulin resistance, obesity and metabolic disorders. In addition, numerous studies have demonstrated rare alleles of these two common variants play a role in the complex pathogenetic mechanism of major diseases supposed to be useful markers to evaluate the connection between PPARγ and metabolic derived disorders.
Given the pivotal roles of PPARγ in regulating metabolism several studies in various ethnic populations including, Caucasians, [17] Mexican-Americans, [18] African-Americans, [19,13] Asian, [20] Eastern Asian, [12] European, [10] Hispanic and non-Hispanic [21] have been  Oligonucleotides used for allelic discrimination assays for Pro12Ala and C1431T were as follows: Allele frequencies of two SNPs were computed using genotype data obtained from these healthy controls and were compared with those reported in other populations. Each SNP was tested for Hardy-Weinberg equilibrium (HWE) conformity. The statistical significance of the differences between genotypic distributions in populations was tested by the Chi-square (χ 2 ) or Fisher's exact test using the SPSS 16.0 statistical package. (SPSS Inc., Chicago, IL) P < 0.05 was considered to be significant.

Results
The genotype characteristics of studied individuals are shown in Table 1 Tables 2   and 3 respectively.

Discussion
The present study represents the examination of the  populations. On the other hand, C1431T polymorphism has not been studied in our population before, so this is why we selected these two common variants to evaluate in our population.    Table 2 except for two reports from European, [31,32] one from Italy, [33] two in African-American, [35] and one from sub-Saharan African population. [35] Allelic but not genotypic frequencies in one report from European, [32] one from Caucasian, [17] one from Hispanic [21] and one from Indian [28] showed significant differences from what we found in our Iranian population. In all the populations the frequency of rare allele homozygote's (TT) was low (0-7%), but there was one exception for a Chinese population that showed more than 12% frequency that it might be due to small sample size. [24] In general, Chinese and other Asian populations showed a higher frequency than others. In this case, our data was different from those of Asian populations and was similar to those in Australian, European and Caucasian.
One report from Europe and one within a Caucasian population showed significance differences in allelic but not genotypic frequencies with our results. [38,30] As shown in Table 3 nations. [41] Several studies have been undertaken on the association between the Pro12Ala and C1431T mutations with numerous diseases such as cardiovascular disease, cancer, diabetes, inflammation on various populations and the results showed controversy. The controversial findings related to these polymorphisms may be attributable to genetically differences in populations.
Therefore, it is necessary to study in different populations to reach a general consensus in this field.
An important limitation in our study is the relatively small sample size. Further association study with a larger population base is needed to confirm our results and may be useful in understanding these SNPs roles in pathology of the main disease in the future.

Conclusion
Statistical differences in the distribution of two common polymorphisms of PPARγ gene between Iranian population and others showed the importance of studying these SNPs in relation to some major diseases. Regards to Iranian different genetic with some other populations in Asia and other continents, it seems that our study confirms this difference concerning the two common PPARγ polymorphisms.