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Cloning and expression of the gene for a fibronectin‐binding protein from Staphylococcus aureus.

The gene encoding the fibronectin‐binding protein (FNBP) from Staphylococcus aureus strain 8325‐4 was isolated from a gene bank in pBR322. The original clone, containing a 6.5‐kb insert, gave a functional product present in the periplasm of Escherichia coli. Analysis of polypeptides isolated after affinity chromatography on fibronectin‐Sepharose followed by ion‐exchange chromatography revealed two gene products, 87 and 165 kd in mol. wt. The amino acid compositions of these two polypeptides and a native FNBP from S. aureus strain Newman were very similar. Antibodies raised against the native FNBP from strain Newman precipitated the 125I‐labelled 165‐kd polypeptide, and unlabeled 165‐ and 87‐kd polypeptides as well as native FNBP inhibited the immunoprecipitation reactions. The region of the fnbp‐gene encoding the fibronectin‐binding activity has been identified and subcloned in an expression vector based on the staphylococcal protein A gene. The resulting product in E. coli is an extracellular fusion protein consisting of two IgG‐binding domains of protein A followed by a fibronectin‐binding region. The fusion protein binds to fibronectin and completely inhibits the binding of fibronectin to intact cells of S. aureus.