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Dephosphorylation and genome‐wide association of Maf1 with Pol III genes during transcriptional repression

Nutrient deprivation and various stress conditions repress RNA polymerase III (Pol III) transcription in S. cerevisiae. The signaling pathways that relay stress/nutrient conditions converge on the conserved protein Maf1, but how Maf1 integrates environmental signals and couples them to transcriptional repression is largely unknown. Here, we demonstrate that Maf1 is phosphorylated in favorable conditions, whereas diverse unfavorable conditions lead to rapid Maf1 dephosphorylation, nuclear localization, physical association of dephosphorylated Maf1 with Pol III, and Maf1 targeting to Pol III genes genome‐wide. Furthermore, Maf1 point mutants defective in full dephosphorylation are compromised for Pol III association and display maf1Δ phenotypes. Acute repression also confers increased interaction between TFIIIB and TFIIIC in crosslinked chromatin. Taken together, Maf1 appears to integrate environmental conditions and signaling pathways through its phosphorylation state, with stress leading to dephosphorylation, association with Pol III at target loci, alterations in basal factor interactions, and transcriptional repression.