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Seperation and Determination of Glycyrrhizic acid and Liquiritin in Licorice using SPE-RP-HPLC

Glycyrrhic acid (GA) and liquiritin are the two classes of bioactive components in licorice with known pharmacological effects. In our study, different methods were compared to separate GA an-d liquiritin. Solid-phase extraction (SPE) method was verified to separate GA and liquiritin effectively. The method is fast, easy, reliable, and inexpensive; especially in the quality control. Moreover, we built a new method to determine glycyrrhizic acid and liquiritin simultaneously using reversed phase high performance liquid chromatography (RP-HPLC) In the HPLC analyze, gradient elution and two wavelength were used . The regression line for GA was y =15340.6x+12.81, the regression for liquiritin line was y =9269.1x+20.63, The recovery rates were 101.3% and 99.4%; RSD were 1.01% and 0.92%.