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EFFECT OF SOYABEAN DIET ON THE SERUM IMMUNOGLOBULIN PROFILE AND CD4 + COUNT OF MALE DIABETIC RATS CHALLENGED WITH K.PNEUMONIAE

In this study, the effect of soyabean supplemented diet on the immunoglobulin profile and CD 4 + count of male diabetic rats challenged with Klebsiella pneumoniae were evaluated in a total of sixty-four mature albino rats, weighing 150-250 grams. The rats were separated into eight groups of eight rats each. The groups were as follows: A- rats fed on rat feed, B-rats fed on rat feed and soyabean meal, C-diabetes-induced rats feed on rat feed, Ddiabetes-induced rats fed on rat feed and soyabean meal, E-rats fed on rat feed but challenged with Klebsiella pneumoniae, F- rats fed on rat feed and soyabean meal challenged with Klebsiella pneumoniae, G-diabetes induced rats fed on normal rat feed but challenged with Klebsiella pneumoniae and H-diabetes-induced rats fed on rat feed and soyabean meal but challenged with Klebsiella pneumoniae. The study lasted for 28days. At the end of 28 days, the rats were bled and blood collected, processed and the serum obtained used for assessment of immunoglobulin profile and CD4 + T-cell count. Immunoglobulin profile was carried out using immunoturbidimetric assay with Randox (R) kit, while CD4 + T-cell count was done using CY-flow machine. Results showed that there were significant increase (P< 0.05) in serum lgA, 1gM, 1gG levels and CD4 + T-cell of diabetic rats fed on normal feed and soyabean meal when compared with their counterparts fed with only normal feed. Results of the study suggest that soyabean diet could assist in boosting the serum immunoglobulin levels and CD4 + cell of diabetic rats and hence protecting the