{'fraction_non_alphanumeric': 0.02507598784194529, 'fraction_numerical': 0.005319148936170213, 'mean_word_length': 5.36231884057971, 'pattern_counts': {'":': 0, '<': 0, '<?xml version=': 0, '>': 0, 'https://': 0, 'lorem ipsum': 0, 'www.': 0, 'xml': 0}, 'pii_count': 0, 'substrings_counts': 0, 'word_list_counts': {'cursed_substrings.json': 0, 'profanity_word_list.json': 0, 'sexual_word_list.json': 0, 'zh_pornsignals.json': 0}}
Cytochemical localization of photosystem I in relation to differentiation of the thylakoid membranes during greening of barley leaves.

Changes in distribution of photosystem I (PS I) in the developing thylakoid membranes of greening barley (Hordeum vulgare L. cv. Amagi-Nijo) chloroplasts were studied by a cytochemical method using 3,3'-diaminobenzidine (DAB). The activity was detected on the thylakoid of etiochloroplasts after 1 h of greening. Stacks of a few thylakoids (thought to be an initiation of grana) appeared at about 2-3 h of greening. Until 4 h, the DAB reaction was observed uniformly throughout the whole thylakoid system (stroma and grana thylakoids). After 6 h of greening, The DAB reaction was detected heterogeneously in the etiochloroplasts; more intense on the stroma thylakoid and peripheral grana thylakoid. Further illumination led to more distinct heterogeneity of the DAB reaction. Thus, redistribution of PS I proceeded differentially after onset of grana formation in the developing etiochloroplasts. Measurements of chlorophyll content, light saturation of PS I activity and fluorescence emission spectra of greening barley leaves suggest that redistribution of PS I may reflect developmental changes in assembly of the PS I complex with light-harvesting chlorophyll-protein complex.