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[Effects of rotating cell culture system on constructing of tissue engineering intervertebral nucleus pulposus].

OBJECTIVE
To study the effect of rotating cell culture system (RCCS) on the in vitro constructing of tissue engineering nucleus pulposus.


METHODS
Nucleus pulposus cells from rabbit thoracic and lumbar intervertebral discs were proliferated in vitro to the third generation and seeded into PLGA (poly-lactide-co-glycolide) scaffolds (3 × 10(7)/ml). Experimental group was subjected to the RCCS while the control group was cultured under a normal condition. Both groups were cultured in vitro. The complex of nucleus pulposus and scaffolds was observed after 4 weeks. Tissue texture, scanning electron microscope, type II collagen, content of GAG (glycosaminoglycan) and DNA were assessed.


RESULTS
After 4 weeks, the tissue engineered nucleus pulposus under RCCS were morphologically thicker and bigger. And the content of GAG, DNA and percentage of typeII collagen were more than those in the control group. The structural arrange of tissue engineering nucleus pulposus was visualized directly.


CONCLUSION
The RCCS promotes intervertebral disc cells to secrete collagen and GAG. It may benefit the construction of tissue engineering intervertebral disc.