US 20200246423Al  c19)  United States  c12)  Patent Application Publication  Liu et al.  (54)  STABLE AQUEOUS FORMULATIONS OF  AFLIBERCEPT  c10) Pub. No.: US 2020/0246423 Al  (43) Pub. Date:  Aug. 6, 2020  (71)  Applicant:  Coherus BioSciences Inc.,  Redwood  City, CA (US)  (72)  Inventors:  Jun Liu,  Redwood City, CA (US);  Mark Manning,  Redwood City, CA  (US);  Sekhar R. Kanapuram,  Thousand Oaks, CA (US)  (21)  Appl. No.:  16/566,847  (22)  Filed:  Sep. 10, 2019  Related U.S. Application Data  (63)  Continuation-in-part of application No. 16/297,387,  filed on Mar. 8, 2019, which is a continuation of  application No. 62/640,411, filed on Mar. 8, 2018.  Publication Classification  (51)  Int. Cl.  A61K 38/17  (2006.01)  A61K  47122  (2006.01)  A61K  47118  (2006.01)  A61K  47126  (2006.01)  A61K 9/00  (2006.01)  (52)  U.S. Cl.  CPC ............  A61K 38/179  (2013.01);  A61K  47122  (2013.01);  A61K 9/08  (2013.01);  A61K  47126  (2013.01);  A61K 9/0048  (2013.01);  A61K  471183  (2013.01)  (57)  ABSTRACT  A stable aqueous pharmaceutical formulation comprising a  therapeutically effective amount of aflibercept, wherein the  formulation is free of organic co-solvent and/or free of  buffer; methods for making such a formulation; and methods  of using such a formulation.  96.296  96.634  96.972  97.310  97.648  97.986  ======:::::i::::::::::::::::::::::::::::::::::::::::•r::::::::::::::::::::::::e  -_-_-_-_-_-_-_-_-_-_-- :r ~:::::::::::::::::::::. :,  Response Surface  8.0 