U.S. Patent  Jan.16,2018  Sheet 3 of 6  US 9,868,761 B2  Figure 3.  Calculated and experimental pH, ionic strength, and buffer  capacity curves for acetic acid and tris base buffer system.  Acetic Acid/Tris Base  12  0.4  - ;::  C:  ::i  0.35  :I:  10  C.  :J  0.3  ::::  0  8  s  0.25  ~  :I:  •u  C.  Ill  C.  ..  6  0.2  Ill  :!  (.)  ::i  ...  aJ  :!  0.15  ::i  4  aJ  0.1  ~  .c:  2  - Cl  ,_. ,...  ..  ••••••••,,,,.  - .....  0.05  C:  Cl)  ..  -- ..  ’  ...  ,, ••••••  /  •••••’  Cl)  0  ...  ••-~  0  (.)  "i:  0  0.02  0.04  0.06  0.08  0.1  0.12  ..2  Buffer concentration  (M) 