US 2020/0246423 Al  66  Aug. 6, 2020  TABLE 33-continued  TABLE 34  TABLE 31  TABLE 32  TABLE 35  TABLE 33  Concentration, pH, and Osmolality  [0400]  The aflibercept concentration (mg/mL), pH, and  osmolality (mOSm) of the Block C formulations were  evaluated at  tO. Results are reported in Table 31 below.  Size Exclusion Chromatography  [0401]  The stability of the Block C formulations was  evaluated by measuring the amount of non-degraded afliber› cept present in the formulation at time 0 (t0) and after being  subjected to storage conditions. Size Exclusion Chromatog› raphy was conducted on the formulations and the percentage  of protein present in the main peak (non-degraded afliber› cept) in each formulation is reported in Tables 32-35 below.  SEC results for Block C formulations  after four weeks at 5°  C.  Rel. Area  Rel. Area  Rel. Area  Form  (%), Before  (%),  (%), After  No.  Main Peak  Main Peak  Main Peak  C-1  1.17  98.83  1.17  C-2  1.19  98.81  1.19  C-3  1.18  98.82  1.18  C-4  1.19  98.78  1.19  C-5  1.24  98.76  1.24  C-6  1.20  98.80  1.20  C-7  1.27  98.73  1.27  C-8  1.20  98.80  1.20  C-9  1.32  98.68  1.32  C-10  1.39  98.61  1.39  C-11  1.66  98.34  1.66  C-12  1.78  98.22  1.78  C-13  1.27  98.73  1.27  C-14  1.36  98.64  1.36  C-15  1.24  98.76  1.24  Form  No.  C-1  C-2  SEC results for Block C formulations after one week  at 40°  C.  Rel. Area  Rel. Area  Rel. Area  (%), Before  (%),  (%), After  aflibercept  Main Peak  Main Peak  Main Peak  40  2.66  97.34  0  40  1.75  98.25  0  Form  No.  C-1  C-2  C-3  C-4  C-5  C-6  C-7  C-8  C-9  C-10  C-11  C-12  C-13  C-14  C-15  SEC results for Block C formulations at T - 0  Rel. Area  Rel. Area  Rel. Area  (%), Before  (%),  (%),After  Main Peak  Main Peak  Main Peak  1.56  98.44  0  1.56  98.44  0  1.54  98.46  0  1.56  98.44  0  1.58  98.42  0  1.55  98.45  0  1.63  98.37  0  1.55  98.45  0  1.69  98.31  0  1.80  98.20  0  2.09  97.91  0  2.20  97.80  0  1.62  98.38  0  1.73  98.27  0  n.a  n.a  0  SEC results for Block C formulations  two weeks at 25 °  C.  Rel. Area  Rel. Area  Rel. Area  Form  (%), Before  (%),  (%), After  No.  Main Peak  Main Peak  Main Peak  C-1  1.51  98.49  0  C-2  1.45  98.55  0  C-3  1.80  98.20  0  C-4  1.56  98.44  0  C-5  1.60  98.40  0  C-6  1.59  98.41  0  C-7  1.67  98.33  0  C-8  1.63  98.37  0  C-9  1.83  98.17  0  C-10  1.94  98.06  0  C-11  2.29  97.71  0  C-12  2.53  97.47  0  C-13  1.73  98.27  0  C-14  1.91  98.09  0  C-15  1.64  98.36  0  Form  Final  No.  mOsm  Final pH  Final mg/mL  C-1  306  5.5  38.74  C-2  319  5.5  39.74  C-3  304  6.0  39.54  C-4  314  6.0  39.62  C-5  305  6.1  39.61  C-6  292  6.2  39.69  C-7  307  6.1  40.07  C-8  321  6.1  39.88  C-9  306  6.5  40.24  C-10  340  6.4  39.31  C-11  319  6.9  39.29  C-12  317  7.0  40.04  C-13  312  6.2  39.44  C-14  323  6.5  39.21  C-15  316  6.5  40.80  SEC results for Block C formulations after one week  at 40°  C.  Rel. Area  Rel. Area  Rel. Area  Form  (%), Before  (%),  (%), After  No.  aflibercept  Main Peak  Main Peak  Main Peak  C-3  40  2.89  97.11  0  C-4  40  1.90  98.10  0  C-5  40  2.46  97.54  0  C-6  40  4.09  95.91  0  C-7  40  2.16  97.84  0  C-8  40  2.83  97.17  0  C-9  40  2.69  97.31  0  C-10  40  2.48  97.52  0  C-11  40  3.36  96.64  0  C-12  40  3.49  96.51  0  C-13  40  2.73  97.27  0  C-14  40  2.90  97.10  0  C-15  40  2.79  97.21  0 