Example 7 - Testing for sugar bioavailability Test product # 1 Less refined sugar: Sugar 1 of Example 1 (prepared by controlled wash of massecuite) Test product #2 Reference product Meal matrix Test system: tiny-TIM Sugar bioavailability testing was conducted in the Triskelion in vitro gastro-lntestinal Model (tiny-TIM), which simulates the successive dynamic processes in the stomach and the small intestine. Name: Less refined sugar Available carbohydrate content: ~99 g / 100 g dry product Intake in tiny-TIM: 6.25 g available carbohydrates Name: refined cane sugar (Rohrzucker, from Nordzucker, purchased in Germany) Available carbohydrate content: 100% Intake in tiny-TIM: 6.25 g available carbohydrates A standardized amount of the test product (standardization: related to amount of available carbohydrates) was mixed with artificial saliva and drinking water. After a short pre-incubation at 30 °C,the mixture was put into the stomach compartment of the model together with the gastric residue containing gastric acid and enzymes (amylase, lipase, pepsin). The tiny-TIM system consists of a gastric compartment and one small intestinal compartment connected by peristaltic valves (Figure 5). This model mimics the intraluminal pH, enzyme activity, bile salt concentrations, peristaltic movements, and gastrointestinal transit of the contents. The set-points for gastrointestinal simulation are controlled and monitored by specific computer programs. Released and dissolved food components are removed from the intestinal lumen by semipermeable membrane units connected to the small intestinal compartment. This allows the assessment of the so- Information on the Triskelion tiny-TIM system is available at: