US 2020/0246423 Al  14  Aug. 6, 2020  [0035]  In one embodiment, the stabilizer is a block copo› lymer. In another embodiment, the block copolymer is  poloxamer (a water-soluble nonionic A-B-A and B-A-B  triblock copolymers, where A is poly( ethylene oxide) (PEO)  and Bis poly(propylene oxide) (PPO)). In a further embodi› ment, the poloxamer is one described in Bodratti A M,  Alexandridis P. Formulation of Poloxamers for Drug Deliv› ery.  J Funct Bio mater.  2018; 9(1 ): 11.  [0034]  In one embodiment, the stabilizer is selected from  the group consisting of amino acids, sugars (e.g., sucrose),  polyols, polymers, block copolymers, alkyl saccharides  (e.g., a ProTek alkyl saccharides, octyl glucoside, N-decyl› pyranoside, N-octyl-glucopyranoside, Intravailfi, and those  described in U.S. Pat. Nos. 9,446,134; 8,846,044, 8,226,949;  7,998,927; 8,084,022; 7,425,542; and 10,046,025), and  FMl 000, or any combination thereof.  [0033]  In one embodiment, the formulation has a pH of  about 5.5 to about 7.0 (e.g., or any of the subranges of this  range described herein, e.g., about 5.5, about 5.6, about 5.7,  about 5.8, about 5.9, about 6.0, about 6.1, about 6.2, about  6.3, about 6.4, about 6.5, about 6.6, about 6.7, about 6.8,  about 6.9, or about 7.0). In another embodiment, the pH is  about 6.0 to about 6.5. In yet another embodiment, the pH  is about 6.2.  [0032]  In another embodiment, the invention provides an  aqueous formulation comprising aflibercept and a stabilizer,  wherein the formulation is free of both an organic co-solvent  and a buffer.  Formulations Free of Buffer and Organic Co-Solvent  [0031]  In one embodiment, the formulation is iso-osmolar  relative to the vitreous.  about 2 mM to about 6 mM, about 2 mM to about 4 mM,  about 4 mM to about 10 mM, about 4 mM to about 8 mM,  about 4 mM to about 6 mM, about 6 mM to about 10 mM,  about 6 mM to about 8 mM, or about 8 mM to about 10  mM); beta-hyrdoxybutyrate at a final concentration of about  0.01 mM to about 0.3 mM ( e.g., about 0.01 mM to about 0.2  mM, about 0.01 mM to about 0.1 mM, about 0.01 mM to  about 0.05 mM, about 0.05 mM to about 3 mM, about 0.05  mM to about 0.2 mM, about 0.05 mM to about 0.1 mM,  about 0.1 mM to about 3 mM, about 0.1 mM to about 0.2  mM, or about 0.2 mM to about 0.3 mM); copper ion at a  concentration of about 0.1 µM  to about 1.0 µM  ( e.g., about  0.1 µM to about 0.8 µM, about 0.1 µM to about 0.6 µM,  about 0.1 µM  to about 0.4 µM, or about 0.1 µM  to about 0.2  µM), zinc ion at a final concentration of about 0.1 µM to  about 10 µM (e.g., about 0.1 µM  to about 8 µM, about 0.1  µM  to about 6 µM, about 0.1 µM  to about 4 µM, or about 0.1  µM  to about 2 µM); selenium ion at a final concentration of  about 0.01 µM  to about 0.2 µM  ( e.g., about 0.01 µM  to about  0.15 µM, about 0.01 µM  to about 0.10 µM,  or about 0.01 µM  to about 0.5 µM), iron ion at a final concentration of about  0.01 µM  to about 8 µM  (e.g., about 0.01 µM  to about 6 µM,  about 0.01 µM  to about 4 µM, about 0.01 µM  to about 2 µM,  about 0.01 µM  to about 1 µM, about 0.01 µM  to about 0.05  µM), ferritin at a final concentration of about 0.1 µM to  about 50 µM  (e.g., 0.1 µM  to about 40 µM, about 0.1 µM  to  about 30 µM, about 0.1 µM  to about 20 µM, about 0.1 µM  to about 10 µM, about 0.1 µM  to about 5 µM, about 0.1 µM  to about 1 µM); and transferrin at final concentration of  about 0.01 µM  to about 0.2 µM  ( e.g., about 0.01 µM  to about  0.15 µM, about 0.01 µM  to about 0.10 µM, about 0.01 µM  to about 0.05 µM).  [0037]  In one embodiment, the stabilizer is an amino acid  ( e.g., an aromatic amino acid, e.g., tyrosine, phenylalanine,  and tryptophan). In another embodiment, the amino acid is  selected from the group consisting of glycine, arginine, and  praline or combinations thereof. The amino acids may be  present at a concentration of about 5-300 mM ( e.g., about 25  mM to about 280 mM, about 25 mM to about 260 mM,  about 25 mM to about 240 mM, about 25 mM to about 220  mM, about 25 mM to about 200 mM, about 25 mM to about  180 mM, about 25 mM to about 160 mM, about 25 mM to  about 140 mM, about 25 mM to about 120 mM, about 25  mM to about 100 mM, about 25 mM to about 95 mM, about  25 mM to about 90 mM, about 25 mM to about 85 mM,  about 25 mM to about 80 mM, about 25 mM to about 75  mM, about 25 mM to about 70 mM, about 25 mM to about  65 mM, about 25 mM to about 60 mM, about 25 mM to  about 55 mM, about 25 mM to about 50 mM, about 25 mM  to about 45 mM, about 25 mM to about 40 mM, about 25  mM to about 35 mM, about 25 mM to about 30 mM, about  30 mM to about 300 mM, about 30 mM to about 280 mM,  about 30 mM to about 260 mM, about 30 mM to about 240  mM, about 30 mM to about 220 mM, about 30 mM to about  200 mM, about 30 mM to about 180 mM, about 30 mM to  about 160 mM, about 30 mM to about 140 mM, about 30  mM to about 120 mM, about 30 mM to about 100 mM,  about 30 mM to about 95 mM, about 30 mM to about 90  mM, about 30 mM to about 85 mM, about 30 mM to about  80 mM, about 30 mM to about 75 mM, about 30 mM to  about 70 mM, about 30 mM to about 65 mM, about 30 mM  to about 60 mM, about 30 mM to about 55 mM, about 30  mM to about 50 mM, about 30 mM to about 45 mM, about  30 mM to about 40 mM, about 30 mM to about 35 mM,  about 35 mM to about 300 mM, about 35 mM to about 280  mM, about 35 mM to about 260 mM, about 35 mM to about  240 mM, about 35 mM to about 220 mM, about 35 mM to  about 200 mM, about 35 mM to about 180 mM, about 35  mM to about 160 mM, about 35 mM to about 140 mM,  about 35 mM to about 120 mM, about 35 mM to about 100  mM, about 35 mM to about 95 mM, about 35 mM to about  90 mM, about 35 mM to about 85 mM, about 35 mM to  about 80 mM, about 35 mM to about 75 mM, about 35 mM  to about 70 mM, about 35 mM to about 65 mM, about 35  mM to about 60 mM, about 35 mM to about 55 mM, about  35 mM to about 50 mM, about 35 mM to about 45 mM,  about 35 mM to about 40 mM, about 40 mM to about 300  mM, about 40 mM to about 280 mM, about 40 mM to about  260 mM, about 40 mM to about 240 mM, about 40 mM to  about 220 mM, about 40 mM to about 200 mM, about 40  mM to about 180 mM, about 40 mM to about 160 mM,  about 40 mM to about 140 mM, about 40 mM to about 120  mM, about 40 mM to about 100 mM, about 40 mM to about  95 mM, about 40 mM to about 90 mM, about 40 mM to  about 85 mM, about 40 mM to about 80 mM, about 40 mM  to about 75 mM, about 40 mM to about 70 mM, about 40  mM to about 65 mM, about 40 mM to about 60 mM, about  40 mM to about 55 mM, about 40 mM to about 50 mM,  about 40 mM to about 45 mM, about 45 mM to about 300  mM, about 45 mM to about 280 mM, about 45 mM to about  260 mM, about 45 mM to about 240 mM, about 45 mM to  about 220 mM, about 45 mM to about 200 mM, about 45  mM to about 180 mM, about 45 mM to about 160 mM,  about 45 mM to about 140 mM, about 45 mM to about 120  [0036]  In one embodiment, the stabilizer is a sugar. In  another embodiment, the stabilizer is sucrose. 