US 2020/0246423 Al  59  Aug. 6, 2020  TABLE 9  TABLE 10-continued  TABLE 11  TABLE 10  TABLE 12  [0387]  The chemical stability of the Block A formulations  was evaluated by measuring the charge variants present in  the formulation after being subjected to storage conditions.  The relative areas for each  of  the twelve features detected by  icIEF are listed for samples from Block  A. These samples  were frozen and  shipped before analysis.  Results  are  reported in Table 12-15 below.  Imaging Capillary Isoelectric Focusing (icIEF)  (2) Faint Schlieren line during analysis (middle of screen)- :rvfVSS Not detected  (1)  Faint Schlieren line during analysis (Top ofscreen)- :rvfVSS Not detected  Note:  (4) Need time stamp after start after 26%  (3) Faint Schlieren line during analysis (middle ofscreen)-:rvfVSS Not detected  (2) High Particle counts  (1)  Faint Schlieren line during analysis (Top of screen)-:rvfVSS Not detected  Note:  Note:  (1)  Schlieren Lines in middle during purge  (2) Faint Schlieren line during analysis (Top of screen)- :rvfVSS Not detected  (3) Faint Schlieren line during analysis (Top of screen)- :rvfVSS Not detected  (4) Faint Schlieren line during analysis (middle ofscreen)- :rvfVSS Not detected  MF! results for Block A formulations stored for one  week at 40°  C.  Size Range Summary  Total  Particle  Form.  Cone.  ;,2  µm  a,5  µm  ;,10  µm  ;,25  µm  Block  (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  A-1  67128  11269  888  244  33  A-2  158216  39837  6318  1066  33  A-3  53449  10625  699  100  22  A-4  4663  1288  222  33  0  A-5  91332  22128  2820  333  22  A-6  38727  8383  1310  289  22  A-7  52650  11913  1155  100  0  A-8  81095  19375  2143  289  33  A-9  84271  21184  2598  289  78  A-10 (1)  45588  9282  899  78  0  A-11 (2)  104489  23805  3286  555  33  A-12  70392  14700  2165  378  33  A-13 (3)  78120  17043  1776  200  0  A-14 (4)  127643  28721  4324  546  15  A-15  62631  13090  1632  322  0  A-16  55403  11158  1532  311  44  A-17  134478  23005  3286  677  56  Eylea  7272  1421  244  44  0  Form.  Block  A-16  A-17  Eylea  MF! results for Block A formulations stored for two  weeks at 25°  C.  Size Range Summary  Total  Particle  Cone.  ;,2  µm  a,5  µm  ;,10  µm  (#/mL)  (#/mL)  (#/mL)  (#/mL)  28135  4896  600  100  27524  6495  788  144  5041  1077  122  33  ;,25  µm  (#/mL)  22  0  22  MF! results for Block  A  formulations stored for four  weeks at 5°  C.  Size Range Summary  Total  Particle  Form.  Cone.  ;,2  µm  a,5  µm  ;,10  µm  ;,25  µm  Block  (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  A-1  35130  7517  866  155  11  A-2  47742  10392  966  67  0  A-3  15011  3042  344  33  11  A-4  58645  13657  1332  67  0  A-5  24915  5662  422  44  0  A-6  48742  10992  1244  155  0  A-7  34252  7483  677  100  22  A-8  41691  10237  1321  189  22  A-9  24238  6417  922  144  0  A-10 (1)  27302  6795  677  67  11  A-11  29445  7317  611  67  0  A-12  16632  3409  333  22  0  A-13 (2)  24759  6795  977  233  11  A-14  32964  6395  799  122  11  A-15  20551  4175  489  111  11  A-16  29345  5885  655  89  11  A-17  37383  9693  1044  78  0  Eylea  4597  1066  233  22  11  Form.  Block  A-1  A-2 (1)  A-3  A-4  A-5  A-6  A-7  A-8  A-9 (2)  A-10 (3)  A-11  A-12  A-13 (4)  A-14  A-15  MF! results for Block  A  formulations stored for two  Total  Particle  Cone.  (#/mL)  28434  47909  31554  30655  48575  70381  6728  57924  68682  18942  20529  30333  31410  35829  86847  weeks at 25°  C.  Size Range Summary  ;,2  µm  a,5  µm  (#/mL)  (#/mL)  5718  555  10170  1010  7084  822  7550  899  10192  1021  16388  1554  1732  366  13224  1121  16932  1610  4508  400  4719  555  7061  1210  6973  899  8205  1166  20529  3464  ;,10  µm  ;,25  µm  (#/mL)  (#/mL)  33  0  111  0  233  22  122  0  155  11  200  0  11  0  78  11  178  11  22  0  44  11  266  89  100  11  178  0  500  44  Formulation Block  A-1  A-2  ic!EF results for Block  A  formulations at T - 0  4.6  4.9  2  6.4  6.2  Rel. Area(%) Smoothing (Group)  4  7  9  10  11  12  7.4  12.5  14.8  15.5  13.1  11.4  7.9 4.2 1.7 0.5  7.0  13.0  14.1  14.9  14.2  11.4  7.7 4.2 1.7 0.6 