0 /6 /3 /2 2 /3 5 /6 0 /3 2 /3 4 /3 5 /3  (rad) 0 /6 /3 /2 2 /3 5 /6  0  10  20  30  40  50  60 F (kJ/mol)  (rad) FIG. 3: Puckering free energy landscape (already corrected with the results from the umbrella sampling phase) of β -d- Glc using the standard 45a4 parameter set. Upper panel: the ( θ, φ ) plane is shown, with isolines drawn every 10 kJ/mol starting from the absolute minimum. Lower panel: projection of the free energy profile onto the φ = 0 plane. Darker colors correspond to lower energies. 35