(IO)  Patent No.:  US 7,807,473 B2  (45)  Date of Patent:  Oct. 5, 2010  c12)  United States Patent  Potyrailo et al.  (54)  MATERIAL COMPOSITIONS FOR SENSORS  FOR DETERMINATION OF CHEMICAL  SPECIES AT TRACE CONCENTRATIONS  AND METHOD OF USING SENSORS  (75)  Inventors:  Radislav A. Potyrailo,  Niskayuna, NY  (US);  Timothy M. Sivavec,  Clifton Park,  NY (US);  Caibin Xiao,  Harleysville, PA  (US);  Theodore J. Cecconie,  Buckingham, PA (US);  Lamyaa Hassib,  Niskayuna, NY (US);  Andrew M.  Leach,  Clifton Park, NY (US);  David B.  Engel,  The Woodlands, TX (US)  (73)  Assignee:  General Electric Company,  Schenectady, NY (US)  ( *)  Notice:  Subject to any disclaimer, the term ofthis  patent is extended or adjusted under 35  U.S.C. 154(b) by 1066 days.  (21)  Appl. No.:  11/259,506  (22)  Filed:  Oct. 26, 2005  (51)  Int. Cl.  GOIN 21100  (2006.01)  (52)  U.S. Cl.  .......................  436/164;  436/166; 436/167;  436/169; 436/171; 436/172; 436/800; 436/805;  422/50  (58)  Field of Classification Search  ................. 436/163,  436/164, 800, 805; 422/85  See application file for complete search history.  FOREIGN PATENT DOCUMENTS  OTHER PUBLICATIONS  (57)  ABSTRACT  17 Claims, 15 Drawing Sheets  (56)  References Cited  U.S. PATENT DOCUMENTS  5,482,866  A  *  1/1996 Denton et al .................. 436/79  5,744,794 A *  4/1998 Michie et al. .......... 250/227.16  5,790,627  A  *  8/1998 Iketaki ........................ 378/43  6,011,882  A  *  1/2000 Dasgupta et al. .............. 385/ 12  3,998,878  A  *  12/1976  4,503,156  A  *  3/1985  5,005,572  A  4/1991  5,116,759 A  *  5/1992  Hearon et  al ................ 562/577  Y  amazato et al. ............. 436/79  Raemer eta!.  Klainer et al.  ........... 435/287.2  A method of quantitatively measuring the concentration of a  chemical species in a sample solution with a sensor film. A  hydrogel sensor film is prepared having a chemical compo› sition comprising an indicator that changes its optical prop› erty in the ultra-violet, visible or near-infrared spectral range  upon being exposed to the chemical species in the sample  solution. The film is exposed to a fixed amount of the sample  solution. The concentration of the chemical species in the  sample solution is quantified using the average absorbance  measured from the sensor film.  (Continued)  Primary Examiner-Jill  Warden  Assistant Examiner-Dean  Kwak  (74)  Attorney, Agent, or Firm-Wegman,  Hessler &  Vanderburg  Miniaturized Bio sensors Employing Electropolymerized Permselec› tive Films and Their Use for Creatinine Assays in Human Serum,  Marcel B. Madaras and Richard P. Buck, Anal. Chem. 1996, 68,  3832-3839.*  (Continued)  EP  0 952 451  10/1999  (Continued)  US 2007 /0092973 Al  Apr. 26, 2007  (65)  Prior Publication Data  2.5  2  8  1.5  C:  ~  0  ~  0.5  0  -0.5  -+-control, water  pH  7  --li!I- 1 OD ppm Mo  1  DD,  new standard  -+rso  ---¾-25  ---e-12.5  -+--1.25  400 450 500 550 600 650 700 750 800  wavelength, nm  Typical set of spectra of a molybdate sensor film device at various molybdate  concentrations.  US0078074 73B2 