US 2020/0246423 Al  71  Aug. 6, 2020  TABLE 51  TABLE 49  TABLE 52  TABLE 53  TABLE 50  Note:  (l)Need Time stamp, 90%- insufficent sample caused bottom right screen schlieren lines  due to illumination error  (2)Need Time stamp, 80%- insufficent sample caused bottom right screen schlieren lines  due to illumination error  [0413]  The stability of the Block D formulations was  evaluated by measuring the particles present in the formu› lation after being subjected to storage conditions. These  samples were shipped frozen for MFI analysis and seem to  have survived the shipping without any significant damage.  Results are reported in Tables 50-53 below.  Micro-Flow Imaging  [0412]  After one week at 40°  C., the monomer content  decreased to 97-98%, similar to what was observed for  previous blocks. No fragmentation is noted and the losses  are solely due to aggregation. At 25°  C. storage there is only  slight changes from the initial levels, suggesting that these  types of excipients (amino acids, salts) are good stabilizers  for aflibercept. For samples stored at 5°  C. for four weeks,  little degradation is seen as well.  [0411]  All of these formulations exhibit monomer con› tents near 99% at TO, as was seen with other blocks.  SEC results for Block D formulations after  four weeks at 5°  C.  Rel. Area  Rel. Area  Rel. Area  Form  (%), Before  (%),  (%), After  No.  Main Peak  Main Peak  Main Peak  D-1  1.13  98.87  0  D-2  1.07  98.93  0  D-3  1.02  98.98  0  D-4  1.00  99.00  0  D-5  1.06  98.94  0  D-6  0.96  99.04  0  D-7  1.08  98.92  0  D-8  0.96  99.04  0  D-9  1.10  98.90  0  D-10  1.08  98.92  0  D-11  1.22  98.78  0  D-12  1.25  98.75  0  Eylea  0.98  99.02  0  MF! results for Block D formulations stored  for one week at 40°  C.  Size Range Summ!!!J:  Form.  Total Particle  ;,2 µm  a,5 µm  ;,10 µm  ;,25 µm  Block  Cone. (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  D-1  48631  13967  2187  433  56  D-2  43412  11403  1743  167  78  D-3  51584  13412  2276  444  33  D-4  42102  11192  2276  333  11  D-5  4597  1388  333  111  22  D-6  40403  11303  2232  433  11  D-7  7939  2332  455  56  22  D-8  22061  4919  755  189  33  D-9  45266  10603  1654  233  22  D-10  30033  7528  1832  500  56  D-11  28901  8161  2121  522  56  D-12  28657  7583  1499  400  33  Eylea  10736  3453  1232  366  22  MF! results for Block D formulations stored  for two weeks at 25  °  C.  Size Range Summ!!!J:  Form.  Total Particle  ;,2 µm  a,5 µm  ;,10 µm  ;,25 µm  Block  Cone. (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  D-1  79885  20751  2620  31unitl  11  D-2  35374  10592  2642  566  44  D-3  31910  7317  877  100  11  D-4  30477  6939  844  133  44  D-5  22439  5263  922  200  0  D-6  21262  5329  655  56  0  D-7  35163  6906  833  44  0  D-8  11647  2631  278  44  0  D-9  2853  777  222  56  11  D-10  19397  4252  533  33  0  D-11  17898  4208  600  67  0  D-12  30233  7272  1010  233  33  Eylea  8305  1865  433  67  11  MF! results for Block D formulations stored  for four weeks at 5  °  C.  Size Range Summm  Form.  Total Particle  ;,2 µm  a,5 µm  ;,10 µm  ;,25 µm  Block  Cone. (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  D-1  76166  22961  4474  600  11  D-2  19419  6440  1454  244  11  D-3  16976  4930  1188  244  0  D-4  13279  3320  344  78  22  D-5  27924  6728  899  122  11  D-6  25481  6606  1232  111  11  D-7(1)  30135  6328  542  40  0  D-8  11447  2987  300  67  33  D-9  26647  6540  999  211  11  D-10  42147  9293  1454  167  0  D-11(2)  47938  14019  2229  273  29  D-12  59289  13823  2198  355  33  Eylea  11170  2665  455  78  0  MF! results for Block D formulations at T - 0  Size Range Summary  Form.  Total Particle  ;,2 µm  a,5 µm  ;,10 µm  ;,25 µm  Block  Cone. (#/mL)  (#/mL)  (#/mL)  (#/mL)  (#/mL)  D-1  17620  4075  611  33  11  D-2  14012  4175  955  233  44  D-3  12358  3175  655  211  11  D-4  10792  2554  389  78  0  D-5  7605  2098  355  133  0  D-6  35385  9460  1876  333  333  D-7  13523  3886  744  144  0  D-8  6195  1510  366  100  0  D-9  19141  5318  966  133  0  D-10  6318  1277  200  33  0  D-11  10514  1887  222  11  11  D-12  16632  3930  655  144  56  Eylea  4430  844  89  22  0 