Oct. 10, 2002  20  US 2002/0147433 Al  described above. The first vial should be a dichlo› romethane blank. Several "check" standards should be  placed (about every 20th sample) throughout the run to  verify correct operation.  [0213]  5) At the completion of the run, check each  chromatogram to insure proper analysis.  If a problem is  suspected,  trouble  shoot  and  correct.  Reanalyze  samples as needed.  [0214]  Calculations  [0215]  The total micrograms of stearyl alcohol in each  sample extract is calculated based on the relative response of  the stearyl alcohol peak to that of the 1-hexadecanol internal  standard. The ratio of the peak areas is multiplied by the  relative response factor ( determined at time of instrument  calibration) and the micrograms of internal standard in the  extract to yield the total  µg  of stearyl alcohol in a sample.  [0216]  Instrument Calibration  [0217]  Determine the instrumental relative response factor  for the stearyl alcohol and the internal standard based on the  areas of the stearyl alcohol and 1-hexadecanol peaks in the  calibration standard chromatogram.  [0218]  where  [0219]  Areainst GC peak area for the internal standard  [0220]  Areasa GC peak area for the stearyl alcohol  [0221]  weightinst micrograms of the internal standard  used to prepare internal standard/extraction solvent  [0222]  weightsa micrograms of the stearyl alcohol  used to prepare the calibration standard  [0223]  Sample Calculations  [0224]  Calculate the total micrograms of stearyl alcohol in  each sample using the peak areas from the sample chro› matogram in the following equation:  [0225]  where  [0226]  Areainst GC peak area for the internal standard  [0227]  Areasa GC peak area for the stearyl alcohol  [0228]  weightinst micrograms of the internal standard  used to prepare internal standard/extraction solvent  [0229]  Report amount of skin care composition trans› ferred in mg/cm 2  where:  Areasa  weightinst  Total  µg  SA  =  Area;n,,  x  Rf  X  ~  -continued  Composition Transferred=  0.001 x  µg  of stearyl alcohol  (concentration of stearyl alcohol in composition) x (tape area)  [0230]  For the method described above the concentration  of stearyl alcohol in the composition is 41 % and the tape  patch measures 4.4 cmx4.4 cm.  [0231]  VII. Specific Examples  [0232]  The following are specific illustrations which: a)  demonstrate the method of determining chemically suitable  proton donating actives; b) demonstrate preparation of vari› ous embodiments of the present invention; and c) demon› strate the efficacy of the present invention in helping main› tain a wearer’s skin at an acidic  pH.  Composition Transferred=(0.0Olxµg of stearyl alco› hol)/(0.41x4.4  cmx4.4 cm) 0.000126xµg  of stearyl  alcohol  (mg/cm 2 )  EXAMPLE 1  Skin pH Reduction Capability  Response factor  (Rf)  =  Area;m,  X  weight’°  X  10  wetghtinst  Areasa  [0233]  This example is intended to demonstrate the pH  reduction capability of several exemplary proton donating  actives. Table 1 lists examples of potentially suitable proton  donating actives for use in the present invention, baseline  skin pH, and the measured pH after each of the ingredients  was evaluated according to the Skin pH Reduction Test that  is described in the  TEST METHODS  section.  TABLE  I  Baseline  Post Application  Component  Skin pH  Skin pH  LI.pH  Control Lotion (No proton  5.99 – 0.1  5.97 – 0.2  0.2 – 0.1  donating active)  5% Citric Acid  5.72  – 0.1  4.17 – 0.4  1.55 –  .3  10.8% NaH$_{2}$PO 4 •H $_{2}$O  6.01  – 0.1  5.05 – 0.2  0.96 – 0.2  5.6% Poly acrylic Acid 1  (~5,000 MW)  5.92 – 0.1  3.32 – 0.3  2.60 – 0.3  5.6% Polyacrylic Acid 2  (~1,250,000 MW)  5.68 – 0.1  3.35 – 0.2  2.33 – 0.3  EXAMPLE 2  Preparation of an Absorbent Article Having a  Topsheet Comprising a Skin Care Composition  A Preparation of Skin Care Composition  [0234]  1 Available from Aldrich Chemical Co., Inc.  of Milwaukee, Wis. as Catalog Number 19203-1  [0235]  2 Available from Aldrich of Chemical Co.,  Inc. of Milwaukee, Wis. as Catalog Number 30621-5  [0236]  All llpH values for lotions containing a potential  proton donating active ingredient are significantly different  from the llpH value for the control lotion at 95% confidence.  These results clearly indicate that all of the materials tested  in this example are capable of causing a reduction in skin  pH.  [0237]  An exemplary skin care composition (Composition  A)  of the present invention having a suspended proton  donating active has the composition shown in Table 2 below: 