c19)  United States  c12)  Patent Application Publication  Keough et al.  (54)  NEW METHODS AND KITS FOR  SEQUENCING POLYPEPTIDES  c10)  Pub. No.: US 2006/0141632 Al  (43)  Pub. Date:  Jun. 29, 2006  Related U.S. Application Data  (57)  ABSTRACT  Publication Classification  (75)  Inventors:  Thomas Woods Keough,  Cincinnati,  OH (US);  Robert Scott Youngquist,  Mason, OH (US)  (73)  Assignee:  The Procter  &  Gamble Co.  (21)  Appl. No.:  09/906,481  (22)  Filed:  Jul. 16, 2001  (60)  Provisional application No. 60/220,564, filed on Jul.  25,  2000.  (51)  Int. Cl.  GOIN 33/00  (2006.01)  (52)  U.S. Cl.  ................................................................  436/89  Correspondence Address:  Bart S. Hersko  The Procter  &  Gamble Co.  Miami Valley Labs  P.  0.  Box 538707  Cincinnati, OH 45253-8707 (US)  The present disclosure provides methods and kits which are  useful for sequencing polypeptides. The methods involve  derivatization of the N-terminus of the polypeptide or pep› tides thereof. The methods also involve derivatization of the  epsilon amino group of the side-chain of the lysine contain› ing polypeptide or peptides thereof. Mass spectral analysis  of one or more of the resulting derivatized analytes provides  spectra which are readily interpreted through the use of  techniques well-known to the ordinarily skilled artisan. The  present disclosure also describes kits which enhance con› venient performance of the methods.  US 20060141632Al 