1.0alpha7.dev.6.sent
sentence
A 1-kb fragment of PAC clone 8856 was isolated by PCR using the oligonucleotides LF-35 (binding antisense in the PPARγ2 5′-UTR) and the oligonucleotide LF-58 (binding sense at position -1000 of the PPARγ2), was sequenced, and was inserted into EcoRV site of pBluescript (Stratagene, La Jolla, CA).