1.0alpha7.dev.73.sent
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K d for TNP-ATP binding to MBP_6.2CΔ36 at pH 7.5 and 6.5 calculated using Equation 1 for the F obsdata in Fig. 10 A were, at pH 7.5, K d = 6.8 ± 0.6 μM, N o = 0.51 ± 0.02 mol of TNP-ATP/mol of protein and, at pH 6.5,K d = 1.4 ± 0.1 μM. 