Imaging the noncentrosymmetric structural organisation of tissue with   Interferometric Second Harmonic Generation microscopy
  We report the imaging of tendon, a connective tissue rich in collagen type I proteins, with Interferometric Second Harmonic Generation (I-SHG) microscopy. We observed that the noncentrosymmetric structural organization can be maintained along the fibrillar axis over more than 150 {\mu}m, while in the transverse direction it is ~1-15 {\mu}m. Those results are explained by modeling tendon as a heterogeneous distribution of noncentrosymmetric nanocylinders (collagen fibrils) oriented along the fibrillar axis. The preservation of the noncentrosymmetric structural organization over multiple tens of microns reveals that tendon is made of domains in which the fraction occupied by fibrils oriented in one direction is larger than in the other. 
Maxime Rivard, Konstantin Popov, Mathieu Laliberte, Antony   Bertrand-Grenier, Francois Martin, Henri Pepin, Christian P. Pf...
physics.bio-ph physics.optics