Reconciliation of Gene and Species Trees With Polytomies
  Motivation: Millions of genes in the modern species belong to only thousands of `gene families'. A gene family includes instances of the same gene in different species (orthologs) and duplicate genes in the same species (paralogs). Genes are gained and lost during evolution. With advances in sequencing technology, researchers are able to investigate the important roles of gene duplications and losses in adaptive evolution. Because of gene complex evolution, ortholog identification is a basic but difficult task in comparative genomics. A key method for the task is to use an explicit model of the evolutionary history of the genes being studied, called the gene (family) tree. It compares the gene tree with the evolutionary history of the species in which the genes reside, called the species tree, using the procedure known as tree reconciliation. Reconciling binary gene and specific trees is simple. However, both gene and species trees may be non-binary in practice and thus tree reconciliation presents challenging problems. Here, non-binary gene and species tree reconciliation is studied in a binary refinement model.   Results: The problem of reconciling arbitrary gene and species trees is proved NP-hard even for the duplication cost. We then present the first efficient method for reconciling a non-binary gene tree and a non-binary species tree. It attempts to find binary refinements of the given gene and species trees that minimize reconciliation cost. Our algorithms have been implemented into a software to support quick automated analysis of large data sets.   Availability: The program, together with the source code, is available at its online server http://phylotoo.appspot.com. 
Yu Zheng, Taoyang Wu, Louxin Zhang
q-bio.PE