Improvements and simplifications in in-gel fluorescent detection of   proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor   man's fluorescent detection method
  Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method. 
Catherine Aude-Garcia (BBSI), V\'eronique Collin-Faure (BBSI), Sylvie   Luche (BBSI), Thierry Rabilloud (BBSI)
q-bio.GN