An obstruction to smooth isotopy in dimension 4
  Techniques of gauge theory are used to define and compute an invariant of certain diffeomorphisms of 4-manifolds. The invariant vanishes for any diffeomorphism which is smoothly isotopic to the identity. As an application, we give the first example of a diffeomorphism of a simply-connected 4-manifold which is homotopic to the identity map, but not smoothly isotopic to the identity. 
Daniel Ruberman