Measurement of FLux Fluctuations in Diffusion in the Small-Numbers Limit
  Using a microfluidics device filled with a colloidal suspension of microspheres, we test the laws of diffusion in the limit of small particle numbers. Our focus is not just on average properties such as the mean flux, but rather on the features of the entire distribution of allowed microscopic trajectories that are possible during diffusive dynamics. The experiments show that: (1) the flux distribution is Gaussian; (2) Fick's Law --- that the average flux is proportional to the particle gradient --- holds even for particle gradients down to one or zero particles; (3) the variance in the flux is proportional to the sum of the particle numbers; and (4) there are backwards flows, where particles flow up a concentration gradient, rather than down it, and their numbers are well-predicted by theory and consistent with a new Flux Fluctuation Theorem. 
Effrosyni Seitaridou, Mandar M. Inamdar, Rob Phillips, Kingshuk Ghosh   and Ken Dill