Determination and Spectroscopy of Quantum Yields in   Bio/Chemiluminescence via Novel Light-Collection-Efficiency Calibration:   Reexamination of The Aqueous Luminol Chemiluminescence Standard
  We have developed a luminescence-measurement system for liquid bio/chemiluminescence that can obtain quantitative luminescence spectra as the absolute total number of luminescence photons at each wavelength or photon energy and quantum yields. Calibration of light-collection efficiency in the system is performed with a reference double-plate cell. This method is applicable to sample cells of any kind suitable for measurement, which is a great advantage over previous techniques in practical experiments. Using this system, the quantum yield of aqueous luminol chemiluminescence was obtained as 1.23-+0.20%, which is in good agreement with previously reported values. 
Yoriko Ando, Kazuki Niwa, Nobuyuki Yamada, Tsutomu Irie, Toshiteru   Enomoto, Hidehiro Kubota, Yoshihiro Ohmiya, Hidefumi Ak...