Correction of cell-induced optical aberrations in a fluorescence   fluctuation microscope
  We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples. 
Charles-Edouard Leroux, Alexei Grichine, Ir\`ene Wang (LIPhy), Antoine   Delon (LEGI)
physics.bio-ph physics.optics