On the Determination of the He Abundance in Extragalactic H II Regions
  Of the light element abundances capable of testing standard big bang nucleosynthesis, only He4 is measured with an accuracy of a few percent. Thus, it is imperative to establish a comprehensive technique for determining He4 abundances and reliable estimates of the true systematic uncertainties. Helium abundance determinations in H II regions are made from the observations of several distinct He I emission lines and their strengths relative to H I emission lines. With the general availability of large format, linear CCD detectors, the accuracy of relative emission line ratios has improved to the point where several terms in the error budget which were assumed to be negligible may now be important. Here we investigate the estimation of errors in deriving and reporting nebular helium abundances from optical emission line spectra. 
Keith A. Olive and Evan D. Skillman