Design Principle of Gene Expression Used by Human Stem Cells;   Implication for Pluripotency
  Human embryonic stem cells (ESC) are undifferentiated and are endowed with the capacities of self renewal and pluripotential differentiation. Adult stem cells renew their own tissue, but whether they can trans-differentiate to other tissues is still controversial. To understand the genetic program that underlies the pluripotency of stem cells, we compared the transcription profile of ESC with that of progenitor/stem cells of human hematopoietic and keratinocytic origins, along with their mature cells to be viewed as snapshots along tissue differentiation. ESC gene profile show higher complexity with significantly more highly expressed genes than adult cells. We hypothesize that ESC use a strategy of expressing genes that represent various differentiation pathways and selection of only a few for continuous expression upon differentiation to a particular target. Such a strategy may be necessary for the pluripotency of ESC. The progenitors of either hematopoietic or keratinocytic cells also follow the same design principle. Using advanced clustering, we show that many of the ESC expressed genes are turned off in the progenitors/stem cells followed by a further downregulation in adult tissues. Concomitantly, genes specific to the target tissue are upregulated towards matured cells of skin or blood. 
Michal Golan-Mashiach, Jean-Eudes Dazard, Sharon Gerecht-Nir, Ninette   Amariglio, Tamar Fisher, Jasmine Jacob-Hirsch, Bella...