Information limits of optical microscopy: application to fluorescently   labelled tissue section
  The article demonstrates some less known principles of image build-up in diffractive microscopy and their usage in analysis unravelling the smallest localized information about the original object - an electromagnetic centroid. In fluorescence, the electromagnetic centroid is naturally at the position of the fluorophore. The usage of an information-entropic variable - a point divergence gain - is demonstrated for finding the most localized position of the object's representation, generally of the size of a voxel (3D pixel). These spatial pixels can be qualitatively classified and used for reconstruction of the 3D structures with precision comparable with electron microscopy. 
Renata Rychtarikova, Georg Steiner, Michael B. Fischer, and Dalibor   Stys
physics.med-ph physics.optics