Direct observation of DNA dynamics in semi-dilute solutions in   extensional flow
  The dynamic behavior of semi-dilute polymer solutions is governed by an interplay between solvent quality, concentration, molecular weight, and flow type. Semi-dilute solutions are characterized by large fluctuations in concentration, wherein polymer coils interpenetrate but may not be topologically entangled at equilibrium. In non-equilibrium flows, it is generally thought that polymer chains can self-entangle in semi-dilute solutions, thereby leading to entanglements in solutions that are nominally unentangled at equilibrium. Despite recent progress, we still lack a complete molecular-level understanding of these dynamics. In this work, we use single molecule techniques to study the dynamics of semi-dilute solutions of DNA in planar extensional flow, including polymer relaxation from high stretch, transient stretching dynamics in step-strain experiments, and steady-state stretching in flow. Our results are consistent with a power-law scaling of the longest polymer relaxation time in semi-dilute solutions, revealing an effective excluded volume exponent $\nu$ = 0.56. We further studied the non-equilibrium stretching dynamics in extensional flow, with results showing a decrease in transient polymer stretch at moderate Weissenberg number and a milder coil-to-stretch transition in semi-dilute solutions compared to dilute solutions. Interestingly, a unique set of molecular conformations during the transient stretching process for single polymers in semi-dilute solutions is observed, which suggests transient stretching pathways for polymer chains in semi-dilute solutions are qualitatively different than dilute solutions due to intermolecular interactions. Taken together, this work provides a molecular framework for understanding the non-equilibrium stretching dynamics of semi-dilute solutions in strong flows. 
Kai-Wen Hsiao, Chandi Samsal, J. Ravi Prakash, Charles M. Schroeder
cond-mat.soft