Expansion-maximization-compression algorithm with spherical harmonics   for single particle imaging with X-ray lasers
  In 3D single particle imaging with X-ray free-electron lasers, particle orientation is not recorded during measurement but is instead recovered as a necessary step in the reconstruction of a 3D image from the diffraction data. Here we use harmonic analysis on the sphere to cleanly separate the angu- lar and radial degrees of freedom of this problem, providing new opportunities to efficiently use data and computational resources. We develop the Expansion-Maximization-Compression algorithm into a shell-by-shell approach and implement an angular bandwidth limit that can be gradually raised during the reconstruction. We study the minimum number of patterns and minimum rotation sampling required for a desired angular and radial resolution. These extensions provide new av- enues to improve computational efficiency and speed of convergence, which are critically important considering the very large datasets expected from experiment. 
Julien Flamant, Nicolas Le Bihan, Andrew V. Martin, Jonathan H. Manton
physics.data-an