The Role of G4-DNA in the Pairing of Chromosomes
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{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
G-4 DNA is a four-stranded structure involving four parallel,                   identical, guanine-rich sequences, with the guanines                            Hoogsteen-paired.  Such elements are able to crosslink four                     Watson-Crick duplexes.  Since during meiosis the pairing of                     chromosomes after DNA replication involves four copies of                       each chromatid, which must synapse together before                              recombination takes place, we suggest that a self-recognition                   of guanine-rich motifs in DNA to form G4-DNA crosslinks cause                   the synapsis.  G4-DNA crosslinks may also be used to align                      chromosomes in mitosis and in the formation of polytene                         chromosomes.  To test the hypothesis that G4-DNA-forming                        regions serve as identification signals for chromosome                          matching, anti-G4-DNA antibodies will be used to detect G4-                     DNA in chromosomes during meiotic pairing.  Anti-G4-DNA                         antibodies will be developed and used to detect G-4 regions                     that may crosslink chromosomes both with peroxidase and                         fluorescence methods.  This will require extensive use of                       high-resolution microscopy to examine chromosome figures in                     meiotic cells.                                                                                                                                                  The results of this work, if supportive of the working                          hypothesis, will result in a major breakthrough in our                          understanding of a fundamental aspect of genetic inheritance,                   meiotic segregation.
{'FirstName': 'Walter', 'LastName': 'Gilbert', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Walter Gilbert', 'EmailAddress': '6174950760', 'NSF_ID': '000093842', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Harvard University', 'CityName': 'CAMBRIDGE', 'ZipCode': '021385366', 'PhoneNumber': '6174955501', 'StreetAddress': '1033 MASSACHUSETTS AVE STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'LN53LCFJFL45', 'ORG_LGL_BUS_NAME': 'PRESIDENT AND FELLOWS OF HARVARD COLLEGE', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1116', 'Text': 'EUKARYOTIC GENETICS'}
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