Development of an Obligatory  Plasmid Protein Production    System
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
One of the major obstacles to the use of recombinant DNA-containing             organisms for the large-scale production of proteins in commercial              processes is the cells genetic instability.  Genetic instabilities              manifest themselves in the spontaneous formation of non-productive              microbial strains which lower overall yields and preempt the use of             long term continuous culture processing strategies.  Host-vector                systems containing obligatory plasmids will be constructed.  In each            system, the single-stranded binding protein gene will be removed from           the genome of E. coli but provided for the cell by including it on a            plasmid.  Loss of the plasmid should then be fatal to the host cell,            and instability caused by plasmid segregation in reactor systems should         be eliminated.  Production of B-lactmase will be used as a measure of           protein production culture.  A convenient polylinker, a high production         plasmid containing a strong promotor, will be constructed to                    facilityate future cloning of industrial interest.
[{'FirstName': 'Alfred', 'LastName': 'Carlson', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Alfred Carlson', 'EmailAddress': 'alfred.carlson@rose-hulman.edu', 'NSF_ID': '000316648', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Ronald', 'LastName': 'Porter', 'PI_MID_INIT': 'D', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Ronald D Porter', 'EmailAddress': None, 'NSF_ID': '000177630', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Pennsylvania State Univ University Park', 'CityName': 'UNIVERSITY PARK', 'ZipCode': '168021503', 'PhoneNumber': '8148651372', 'StreetAddress': '201 OLD MAIN', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '15', 'CONGRESS_DISTRICT_ORG': 'PA15', 'ORG_UEI_NUM': 'NPM2J7MSCF61', 'ORG_LGL_BUS_NAME': 'THE PENNSYLVANIA STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8613138.xml'}