Engineering Protein Separations on Immobilized Metals
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
The utilization of biological affinity to effect a separation is                the basis of a number of promising processes.  A particularly                   attractive version of the affinity concept utilizes interactions                between proteins and immobilized metals.  The principal                         investigators propose a novel approach to the problem of protein                purification based on their ability to engineer specific protein                structures using genetic methods.  They propose to demonstrate,                 using two proteins of very different surface properties and                     conformations, that specific metal binding sites can indeed be                  incorporated into proteins or attached as removable "tails"                     without loss of biological activity or folded structure.  With                  this alteration, proteins can possibly be engineered for a single-              step purification on metal-containing polymer matrices.
[{'FirstName': 'Frances', 'LastName': 'Arnold', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Frances H Arnold', 'EmailAddress': 'frances@cheme.caltech.edu', 'NSF_ID': '000217529', 'StartDate': '08/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'John', 'LastName': 'Richards', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'John H Richards', 'EmailAddress': 'jhr@starbase1.caltech.edu', 'NSF_ID': '000458274', 'StartDate': '08/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'California Institute of Technology', 'CityName': 'PASADENA', 'ZipCode': '911250001', 'PhoneNumber': '6263956219', 'StreetAddress': '1200 E CALIFORNIA BLVD', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '28', 'CONGRESS_DISTRICT_ORG': 'CA28', 'ORG_UEI_NUM': 'U2JMKHNS5TG4', 'ORG_LGL_BUS_NAME': 'CALIFORNIA INSTITUTE OF TECHNOLOGY', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
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