The Mechanism by Which Ribosomes Form Proteins
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The goal of this proposal is to understand in molecular                         mechanical terms how ribosomes and the macromolecules that                      interact with them function to form proteins.  Specifically,                    the proposed research is to determine how tRNAs and mRNA move                   in relation to each other and to ribosomal structural                           components during the reaction steps by which peptides are                      elongated on ribosomes.  The appraoch is to bind                                fluorescently labeled tRNAs or mRNA to labeled or unlabeled                     ribosomes, then to measure various physical changes before                      and after completion of a specific reaction of the peptide                      elongation cycle.  Probes are covalently attached at specific                   known sites on tRNA, mRNA analogues, ribosomal proteins and                     ribosomal RNA.  The latter are reassembled into functionally                    competent ribosomes.                                                                                                                                            All proteins are formed on ribosomes with mRNA and tRNA by a                    mechanism that appears to be similar for all organisms.                         Thus, knowledge of ribosome structure and function is                           fundamental to an understanding of how proteins, which                          catalyze the myriad reactions that occur in living systems,                     are formed at the molecular level.
{'FirstName': 'Boyd', 'LastName': 'Hardesty', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Boyd A Hardesty', 'EmailAddress': None, 'NSF_ID': '000428567', 'StartDate': '01/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Texas at Austin', 'CityName': 'AUSTIN', 'ZipCode': '787121139', 'PhoneNumber': '5124716424', 'StreetAddress': '110 INNER CAMPUS DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '25', 'CONGRESS_DISTRICT_ORG': 'TX25', 'ORG_UEI_NUM': 'V6AFQPN18437', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF TEXAS AT AUSTIN', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '114400', 'Text': 'Molecular Biophysics'}
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