RUI: Control of Membrane Development
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith A. Verbeke', 'PO_EMAI': None, 'PO_PHON': None}
Sequential changes in gene expression control differentiation in                Bacillus subtilis during sporulation.  Little information exists                on the gene products of early sporulation, when rapid assembly of               unique, intracellular, sporulation-specific membranes occurs,                   i.e. asymmetric spore septum and engulfment membranes, to                       establish the intracellular forespore.  Dr. Andreoli has                        demonstrated 8 unique membrane proteins in forespore membranes                  and isolated genes for 4 of the 8 proteins (S1, S2, E3 and E4) by               screening with specific IgG B. subtilis genomic library.                        Restriction maps for the cloned S1 E3, and E4 inserts were                      constructed. The S1, S2, E3 and E4 genes will be isolated by                    subcloning into lambda ZAP to locate the relevant genes in the                  inserts recovered from the genomic library and the genes will be                mapped in the B. subtilis chromosome. The results should tell                   whether each of the membrane protein genes map at known spo loci,               or others.                                                                      %%%                                                                             Current understanding of B. Subtilis molecular genetics makes                   this cell system ideal for studies of the control of sporulation.               The early stages of this dramatic developmental change involve                  the rapid development of unique, intracellular, sporulation-                    specific membranes. This work will allow the identification of                  and role for some of those proteins.
{'FirstName': 'Anthony', 'LastName': 'Andreoli', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Anthony J Andreoli', 'EmailAddress': None, 'NSF_ID': '000467862', 'StartDate': '04/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'California State L A University Auxiliary Services Inc.', 'CityName': 'LOS ANGELES', 'ZipCode': '900324226', 'PhoneNumber': '3233433648', 'StreetAddress': '5151 STATE UNIVERSITY DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '34', 'CONGRESS_DISTRICT_ORG': 'CA34', 'ORG_UEI_NUM': 'C1ABLRAQTB48', 'ORG_LGL_BUS_NAME': 'CAL STATE LA UNIVERSITY AUXILIARY SERVICES INC', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8820092.xml'}