Cell Interaction-Dependent Neural Development
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Christopher Platt', 'PO_EMAI': None, 'PO_PHON': None}
The long-term objectives of this research plan are to define the                mechanisms of heterotypic cell-cell interactions which regulate                 the normal development and maturation of embryonic neural cells.                Cell differentiation and maturation in neural tissues is a                      plastic process and subject to change in response to                            microenviromental cues.  Hence, inborn developmental errors                     likely result when normal cell relations are disturbed by                       external stimuli or genetic alterations.  Certain aspects of                    neuroglial cell development have been defined which are dependent               on neuronal-glial interactions for normal and timely expression.                Specific probes of cell surface components have been defined                    which interfere with this interaction between heterotypic cells.                The cell surface molecules which react with the inhibitory probes               will be defined and their role in cell-cell communication                       investigated.  Biochemical and immunochemical analyses will be                  conducted to define the molecular interactions involved.  Defined               subcategories of neural cells will be isolated by immunomagnetic                separation.  The defined cell types will be recombined with other               defined populations of cells in vitro under varying controlled                  conditions and the effects of cell interaction inhibitors                       determined.  Biochemical analysis of communication-dependent gene               expression will be measured.  Studies will also be conducted                    in situ to determine whether or not probes which interfere with                 cell development in vitro will also affect development in the                   embryo.
{'FirstName': 'Paul', 'LastName': 'Linser', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Paul J Linser', 'EmailAddress': 'pjl@whitney.ufl.edu', 'NSF_ID': '000274647', 'StartDate': '03/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Florida', 'CityName': 'GAINESVILLE', 'ZipCode': '326111941', 'PhoneNumber': '3523923516', 'StreetAddress': '1523 UNION RD RM 207', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Florida', 'StateCode': 'FL', 'CONGRESSDISTRICT': '03', 'CONGRESS_DISTRICT_ORG': 'FL03', 'ORG_UEI_NUM': 'NNFQH1JAPEP3', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF FLORIDA', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '119000', 'Text': 'DEVELOPMENTAL NEUROSCIENCE'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8819743.xml'}