Helical and Superhelical Structure of DNA
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The experiments proposed in this renewal application                       revolve around three primary goals.  The first is to design an                  enzyme for making positively supercoiled DNA.  It is based on                   the thesis that during transcription a positive superhelical                    debt is built ahead of the replication fork while a negative                    superhelical tension accumulates behind the replication fork.                   The situation is dramatic when both the DNA and polymerase are                  constrained during the act of transcription.  The model for                     such an enzyme is a fusion protein that generates both                          conditions, that is, a protein containing a sequence-specific                   DNA binding domain joined to RNA polymerase.  The second goal                   is to analyze chromatin structure in vivo.  DNA nucleases will                  be cloned under the control of inducible regulatory sequences                   in yeast and in higher eukaryotic cells.  To obviate problems                   in making nucleases in vivo, the strategy is to construct cold                  sensitive mutants that are inactive at low temperature, but                     able to cleave DNA in vivo when the medium is shifted to a                      higher temperature.  The third goal is to make supercoiled RNA.                      The results of the research should enhance our                             understanding of the structure of genes and how they function                   at the molecular level in living systems.
{'FirstName': 'James', 'LastName': 'Wang', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'James C Wang', 'EmailAddress': None, 'NSF_ID': '000405036', 'StartDate': '06/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Harvard University', 'CityName': 'CAMBRIDGE', 'ZipCode': '021385366', 'PhoneNumber': '6174955501', 'StreetAddress': '1033 MASSACHUSETTS AVE STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'LN53LCFJFL45', 'ORG_LGL_BUS_NAME': 'PRESIDENT AND FELLOWS OF HARVARD COLLEGE', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1166', 'Text': 'MOLECULAR BIOCHEMISTRY'}, {'Code': '9200', 'Text': 'US CHINA COOP IN BASIC SCIENCE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8807067.xml'}