Hybridoma Production by Electro-Acoustic Fusion
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{'SignBlockName': 'Kesh S. Narayanan', 'PO_EMAI': None, 'PO_PHON': None}
The use of ultrasonic standing waves to facilitate the production of            mammalian cell-cell hybrids has been demonstrated.  However, no evidence        has been presented to show that such hybrids are cultivable or                  functional.  The proposal seeks support to create mouse-mouse hybridomas        by electroacoustic fusion and by electrofusion, to culture the resulting        hybridomas for determination of relative yield by each method, and to           determine whether such hybridomas secrete antibodies.                                                                                                           Electroacoustic fusion allows the use of physiological solutions in             contrast to electrofusion which requires potentially harmful hypotonic          media, and conventional polyethylene glycol fusion which employs toxic          organic compounds.  Electroacoustic fusion, therefore, offers the               possibility of producing greater yields of viable, functional                   hybridomas.
{'FirstName': 'David', 'LastName': 'Boraker', 'PI_MID_INIT': 'K', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David K Boraker', 'EmailAddress': None, 'PI_PHON': None, 'NSF_ID': '000437957', 'StartDate': '01/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
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{'Code': '5371', 'Text': 'SBIR Phase I'}
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