Function of Sterols in the Metabolism of Saccharomyces      cerevisiae
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'M.  Paige', 'PO_EMAI': None, 'PO_PHON': None}
Sterols have been found to occur in almost all eucaryotic as                    well as a few procaryotic organisms.  With the exception of                     the hormonal rolesl, few studies have addressed the non-                        structural roles for sterols in the cells.  Working with a                      specially constructed yeast sterol auxotroph, least four                        functions for sterols in that organism have now been defined.                   It has become obvious that there is an intimate relationship                    between regulation of sterol synthesis and heme competency.                     This relationship will be explored further in this study.                       Using immunocytochemical and radio-labeling techniques, the                     cellular location of ergosterol will be determined.  This                       will be related to sterol functions and the yeast cell cycle.                   The function of the sterol C5=6 unsaturation will be probed.                    Finally, an interesting mutation allowing an apparent                           decrease in structural specificity for sterol functions will                    be studied.                                                                                                                                                     The proposed research is a part of an overall objective of                      understanding the role of sterols in the general economy of                     the cell.  Yeast is ideally suited for the research because                     of its ease of culture, the well-defined genetic and                            molecular biological procedures, and the wealth of                              biochemical data that is available on this organism.***
{'FirstName': 'Leo', 'LastName': 'Parks', 'PI_MID_INIT': 'W', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Leo W Parks', 'EmailAddress': 'PARKS@MBIO.NCSU.EDU', 'NSF_ID': '000315799', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'North Carolina State University', 'CityName': 'RALEIGH', 'ZipCode': '276950001', 'PhoneNumber': '9195152444', 'StreetAddress': '2601 WOLF VILLAGE WAY', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'North Carolina', 'StateCode': 'NC', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'NC02', 'ORG_UEI_NUM': 'U3NVH931QJJ3', 'ORG_LGL_BUS_NAME': 'NORTH CAROLINA STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1144', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8814387.xml'}