Stabilization of Recombinant Plasmids in Bacillus Subtilis
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken'}
The stability of recombinant DNA (plasmid) in bacteria used for                 commercial scale fermentations is of great concern.  Bacillus                   subtilis is a potentially useful microorganism for the production  of           recombinant DNA products.  However, instability of plasmids in B.               subtilis has limited its usefulness as an industrial cloning                    host.  The overall objective of this study is to analyze genetic,               nutritional and environmental factors that affect the stability of              recombinant DNA in B. subtilis.                                                                                                                                 The PIs propose to quantitate the parameters that govern the                    kinetics of plasmid loss, i.e., to measure                                                                                                                             -  The frequency with which segregational events or                                structural changes in the plasmid occur.                                                                                                                    -  The growth advantage the modified cells have over                               parental cells.                                                                                                                                        Chemostat studies will be employed as a unique tool for                         delineating the relative effects of segregational and structural                plasmid instabilities.  Using state-of-the-art fermentor                        monitoring and estimation methodologies, the on-line detection of               revertants in the recombinant culture will be attempted at an                   early stage to allow for the application of stabilization strategies in         fed-batch and continuous culture.
[{'FirstName': 'Arnold', 'LastName': 'Demain', 'EmailAddress': None, 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Gregory', 'LastName': 'Stephanopoulos', 'EmailAddress': 'gregstep@mit.edu', 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Massachusetts Institute of Technology', 'CityName': 'Cambridge', 'ZipCode': '021394301', 'PhoneNumber': '6172531000', 'StreetAddress': '77 MASSACHUSETTS AVE', 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA'}
{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8711725.xml'}