Genetics of E. coli Development
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
Proteus mirabilis exhibits two biological phenomena that merit                  detailed genetic analysis: (1) Swarm colony development,                        involving regular cycles of bacterial growth, cellular                          differentiation and migration of multicellular groups; and (2)                  the Dienes reaction, whereby two swarms from different strains                  produce specific boundary structures when they encounter each                   other on a common substrate.  These two aspects of Proteus                      biology raise fundamental questions about how multicellular                     biological patterns are generated and how self/non-self                         recognition occurs.  The proposed research entails the use of in                vivo methods for insertional mutagenesis and molecular cloning to               identify segments of the genomes of different P.mirabilis strains               which encode components of the systems that control morphogenesis               and strain identity.  The research will test specific hypotheses                about the role of diffusible substances in guiding swarm colony                 development and about the role of prophage sequences in                         interstrain boundary formation.  The data will provide a                        foundation for a molecular comparison of the many (possible over                a billion) distinct strains which comprise the species P.                       mirabilis, and these results will serve as a basis for                          understanding multicellular controls and how they operate as                    bacteria function in natural environments.
{'FirstName': 'James', 'LastName': 'Shapiro', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'James A Shapiro', 'EmailAddress': 'jsha@midway.uchicago.edu', 'NSF_ID': '000299654', 'StartDate': '06/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Chicago', 'CityName': 'CHICAGO', 'ZipCode': '606375418', 'PhoneNumber': '7737028669', 'StreetAddress': '5801 S ELLIS AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Illinois', 'StateCode': 'IL', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'IL01', 'ORG_UEI_NUM': 'ZUE9HKT2CLC9', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF CHICAGO', 'ORG_PRNT_UEI_NUM': 'ZUE9HKT2CLC9'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1112', 'Text': 'Genetic Mechanisms'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8715935.xml'}