Probing DNA Secondary Structure in vivo with Chiral         Metal Complexes
{'Code': '03090007', 'Directorate': {'Abbreviation': 'MPS', 'LongName': 'Direct For Mathematical & Physical Scien'}, 'Division': {'Abbreviation': 'CHE', 'LongName': 'Division Of Chemistry'}}
{'SignBlockName': 'Jon F. Parcher', 'PO_EMAI': None, 'PO_PHON': None}
This project is in the Chemistry of Life Processes Initiative                   in the Office of Special Projects in Chemistry.  It is under                    the co-direction on Jacqueline Barton (Chemistry) and Lawrence                  Chasin (Biological Sciences) and involves the search for                        understanding of distinct DNA conformations and the mapping of                  binding sites in extra cellular and intra cellular media.  This                 research is important to the understanding of altered DNA                       structure and local conformation along DNA strands.                                                                                                             The overall objective of this research is to relate conforma-                   tional polymorphism of DNA which has been observed in vitro to                  that in the biological environment.  Chiral metal complexes                     which recognize and distinguish the A, B, and Z conformations                   of DNA will be used, upon photoactivation, to cleave the DNA                    strands.  The points of cleavage will be identified by electro-                 phoresis.  This will provide the basis for comparison of in                     vivo conformations of DNA with those previously observed in                     vitro.  Results from this research will answer questions                        concerning whether selected regions of DNA are protected or                     accessible and whether variations in local conformation relate                  to gene expression.
{'FirstName': 'Lawrence', 'LastName': 'Chasin', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Lawrence A Chasin', 'EmailAddress': 'lac2@columbia.edu', 'NSF_ID': '000104201', 'StartDate': '05/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Columbia University', 'CityName': 'NEW YORK', 'ZipCode': '100277922', 'PhoneNumber': '2128546851', 'StreetAddress': '615 W 131ST ST', 'StreetAddress2': 'MC 8741', 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '13', 'CONGRESS_DISTRICT_ORG': 'NY13', 'ORG_UEI_NUM': 'F4N1QNPB95M4', 'ORG_LGL_BUS_NAME': 'THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1966', 'Text': 'SYNTHETIC INORGANIC'}, {'Code': '1982', 'Text': 'BIOLOGICAL CHEMISTRY'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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