DNA/Protein Interactions in the Transcription of 5S rRNA    Genes
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg'}
The nucleic acid/protein interactions that are important in the                 transcriptional activation of 5S rRNA genes will be                             characterized in detail.  The specific questions to be                          addressed are of  considerable interest in the broader context                  of eukaryotic gene expression and regulation.  These studies                    focus on the Xenopus transcription factor 111A (TF 111A) with                   the internal control region (ICR) of 5S rRNA genes.  The                        structural aspects of TF111A that are important in sequence                     specific DNA and RNA binding as well as in transcriptional                      activation will be analyzed using directed mutagenesis                          techniques to produce structurally altered TF111A variants.  A                  saturated set of point mutations will be created in the 5S ICR.                                                                                                 A major goal in the analysis of eukaryotic gene structure and                   function is to understand the mechanisms whereby a specific                     gene is transcriptionally activated, and how these mechanisms                   contribute to genetic regulation.  It has become clear that                     questions relating to transcriptional mechanism and regulation                  can now be restated in biochemical terms.  Such answers will be                 sought by Dr. Setzer.
{'FirstName': 'David', 'LastName': 'Setzer', 'EmailAddress': 'setzerd@missouri.edu', 'StartDate': '03/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Case Western Reserve University', 'CityName': 'CLEVELAND', 'ZipCode': '441064901', 'PhoneNumber': '2163684510', 'StreetAddress': 'Nord Hall, Suite 615', 'CountryName': 'United States', 'StateName': 'Ohio', 'StateCode': 'OH'}
{'Code': '1144', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8819286.xml'}