Mechanism-Based Probes of Thiamin-Dependent Enzymes
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The long term goal of this proposal is the elucidation of the                   structure and mechanism of action of thiamin diphosphate-                       dependent pyruvate decarboxylating enzymes; pyruvate                            decarboxylase and pyruvate dehydrogenase complex.  The latter                   enzyme is at a key junction in metabolism; a thorough                           understanding of its structure and mechanism is of                              fundamental biochemical importance.  The enzymes will be                        purified in part by immunoaffinity chromatographic methods.                     The specific objectives are to:                                                                                                                                 1.  design and synthesize mechanism-based inhibitors identify                   the sequence of the coenzyme and substrate binding sites and                    determine the location (including surrounding sequence) of                      the key Cys SH's of the enzymes.                                                                                                                                2.  determine the structure of the key enzyme-bound enamine                     using electronic spectroscopy.  Ascertain its reactivity                        vis-a-vis protonation, disulfides, and flavin, in reactions                     mimicking all the important classes of thiamin                                  diphosphate-dependent alpha-keto acid decarboxylating                           enzymes.
{'FirstName': 'Frank', 'LastName': 'Jordan', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Frank Jordan', 'EmailAddress': 'frjordan@andromeda.rutgers.edu', 'NSF_ID': '000215079', 'StartDate': '10/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Rutgers University New Brunswick', 'CityName': 'NEW BRUNSWICK', 'ZipCode': '089018559', 'PhoneNumber': '8489320150', 'StreetAddress': '3 RUTGERS PLZ', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New Jersey', 'StateCode': 'NJ', 'CONGRESSDISTRICT': '12', 'CONGRESS_DISTRICT_ORG': 'NJ12', 'ORG_UEI_NUM': 'M1LVPE5GLSD9', 'ORG_LGL_BUS_NAME': 'RUTGERS, THE STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': 'Rutgers University Newark', 'CityName': 'NEWARK', 'StateCode': 'NJ', 'ZipCode': '071023026', 'StreetAddress': '123 WASHINGTON ST STE 510', 'CountryCode': 'US', 'CountryName': 'United States', 'StateName': 'New Jersey', 'CountryFlag': '1', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_PERF': 'NJ10'}
[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8709758.xml'}