Calmodulin and the Regulation of Bioelectricity
{'Code': '08060200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DCB', 'LongName': 'Division of Cellular Biosciences'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
Dr. Dedman is interested in the molecular mechanism of the action of            calmodulin, a calcium binding protein, in modulating electrical activity        in excitable cells.  The electric organ of the eel contains large               amounts of calmodulin, has large cells and has been used to study               electrical activity.  In this pilot project, Dr. Dedman will develop            techniques to perform whole cell perfusions while monitoring the                potential differences across the anterior and posterior electrocyte             membranes.  The size of the electrocyte (2mm x 0.15mm), is ideal to             apply whole cell perfusion techniques.  The perfusion micropipettes will        be made with an opening of about 0.02mm OD.  The cell after                     identification with Hoffman modulation contrast optics will be impaled          at each lateral surface, and the flow of the internal perfusion system          controlled by hydrostatic difference in the affluent/effluent                   reservoirs.  The perfusate will be passed through mellitin (an inhibitor        of calmodulin) sepharose and calmodulin sepharose columns to remove             calmodulin and calmodulin target proteins.  This solution will be               repleted with calmodulin and calmodulin target proteins, or antibodies          to calmodulin or the target proteins.  During the perfusions, the               resting membrane potential differences and the action potentials will be        measured.  This pilot project will determine whether whole cell                 perfusion can bridge molecular biology with a defined physiological             event.
{'FirstName': 'John', 'LastName': 'Dedman', 'PI_MID_INIT': 'R', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'John R Dedman', 'EmailAddress': None, 'NSF_ID': '000233296', 'StartDate': '09/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'The University of Texas Health Science Center at Houston', 'CityName': 'HOUSTON', 'ZipCode': '770303870', 'PhoneNumber': '7135003999', 'StreetAddress': '7000 FANNIN ST FL 9', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '18', 'CONGRESS_DISTRICT_ORG': 'TX18', 'ORG_UEI_NUM': 'ZUFBNVZ587D4', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT HOUSTON', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '111700', 'Text': 'CELLULAR PHYSIOLOGY'}, {'Code': '914500', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8807095.xml'}