Affinity Labeling of Purine Nucleotide Sites in Proteins
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The goal of this award is to develop the tools which will                  allow a systematic exploration of the amino acid residues in                    the purine nucleotide binding sites of proteins.  Such sites                    will be affinity labeled using reagents which contain                           alkylating groups capable of reacting covalently with the                       protein.  The purine nucleotide moiety will thus provide the                    specifity for chemical modification of the proteins within the                  binding sites.  The effects of these reagents will be examined                  on three proteins, pyruvate kinase, a membrane-bound ADP                        receptor protein from human platelets and cAMP                                  phosphodiesterase.                                                                   The results of the research should enhance our                             understanding of the structure and architecture of the active                   sites of proteins, and how these biomacromolecules catalyze or                  regulate the myriad reactions that occur in living systems.
{'FirstName': 'Roberta', 'LastName': 'Colman', 'PI_MID_INIT': 'F', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Roberta F Colman', 'EmailAddress': 'rfcolman@udel.edu', 'NSF_ID': '000179260', 'StartDate': '06/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Delaware', 'CityName': 'NEWARK', 'ZipCode': '197160099', 'PhoneNumber': '3028312136', 'StreetAddress': '220 HULLIHEN HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Delaware', 'StateCode': 'DE', 'CONGRESSDISTRICT': '00', 'CONGRESS_DISTRICT_ORG': 'DE00', 'ORG_UEI_NUM': 'T72NHKM259N3', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF DELAWARE', 'ORG_PRNT_UEI_NUM': 'T72NHKM259N3'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8804706.xml'}