Communication Mediating Development in Dictyostelium
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith Plesset', 'PO_EMAI': None, 'PO_PHON': None}
Cell-cell contact and cAMP are critical regulators of Dictyostelium        discoideum development.  Dr. Fontana has shown that cell-cell contact           evokes cAMP secretion and alters amoebal responsiveness to subsequent           applications of cAMP.  These observations provide a biochemical,                quantitatable assay for a contact-mediated event and suggest that, in           some instances, cell-cell contact may regulate development by altering          cAMP levels.  Her first objective involves the elucidation of the               mechanism through which cell-cell contact elicits these responses.  The         following will be examined to determine their involvement in the                transduction of the contact stimulus: the adenylate cyclase, a GTP-             binding protein, the ras protein, a cytoplasmic component, and IP3.  The        contact receptor and ligand will be identified using biochemical and            immunological methods.  The second objective is the implication of these        contact-mediated responses in the regulation of development.  Mutant            analysis will be employed.  The third objectiv is the characterization          of Dictyostelium in vivo cyclic nucleotide metabolism.  A technique             involving 18O labeling will be utilized.                                            Understanding the mechanisms by which chemical signals and cell-cell        contact regulate development in this primitive eukaryotic organism may          lead to a greater understanding of the mechanisms by which these                regulate development in humans.
{'FirstName': 'Donna', 'LastName': 'Fontana', 'PI_MID_INIT': 'R', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Donna R Fontana', 'EmailAddress': 'donna@microb.med.umn.edu', 'NSF_ID': '000308118', 'StartDate': '11/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Minnesota-Twin Cities', 'CityName': 'MINNEAPOLIS', 'ZipCode': '554552009', 'PhoneNumber': '6126245599', 'StreetAddress': '200 OAK ST SE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MN05', 'ORG_UEI_NUM': 'KABJZBBJ4B54', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF MINNESOTA', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1111', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8711153.xml'}