Molecular Biology of Proteolysis in T4-Infected Cells
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
This proposal focuses on the molecular biology of protein                       degradation in phage T4-infected Escherichia coli bacteria.  The                specific aims of this proposal include (a) the identification of                T4 genes in addition to pinA that function to block E. coli                     proteolytic activities.  (b) The characterization of T4 mutants                 that fail to block protein degradation.  The methodology involves               standard molecular biological and biochemical procedures and, in                addition, novel screening and selection strategies based on the                 degradation of specific proteins.  This research should lead to                 a broader knowledge of virus - host cell interactions, to an                    increased understanding of the molecular bases by which E. coli                 proteases recognize their substrates, and to an ability to reduce               proteolysis in E. coli and, thereby, to stabilize proteins of                   medical and scientific importance produced in E. coli bacteria.
{'FirstName': 'Lee', 'LastName': 'Simon', 'PI_MID_INIT': 'D', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Lee D Simon', 'EmailAddress': None, 'NSF_ID': '000120164', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Rutgers University New Brunswick', 'CityName': 'NEW BRUNSWICK', 'ZipCode': '089018559', 'PhoneNumber': '8489320150', 'StreetAddress': '3 RUTGERS PLZ', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New Jersey', 'StateCode': 'NJ', 'CONGRESSDISTRICT': '12', 'CONGRESS_DISTRICT_ORG': 'NJ12', 'ORG_UEI_NUM': 'M1LVPE5GLSD9', 'ORG_LGL_BUS_NAME': 'RUTGERS, THE STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1112', 'Text': 'Genetic Mechanisms'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8818950.xml'}