Redox Proteins of the Membranes of Glyoxysomes
{'Code': '08070800', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Robert Uffen', 'PO_EMAI': None, 'PO_PHON': None}
A redox system is present in the membranes of glyoxsomes                   from the endosperm of germinating castor bean (Ricinus communis                 L.).  This electron transport system is thought to consist of a                 transmembrane flavoprotein (NADH:ferrichyanide reductase) and                   cytochrome b5 in the cytoplasmic face of the membrane.  It is                   hypothesized that the flavoprotein accepts electrons from NADH at               the matrix face and transfers those electrons to cytochrome b5 or               a cytosolic acceptor at the cytoplasmic face.  The protein                      responsible for the NADH:ferricyanide reductase activity will be                isolated from glyoxysomal membranes and characterized.  Another                 redox protein, cytochrome b5, will also be isolated.  The kinetic               characteristics of the isolated proteins will be compared to the                redox activities in the intact glyoxysomal membranes.  Possible                 endogenus electron acceptors will be identified.  Antibodies to                 the redox proteins will be generated and used together with                     inhibitors and non-penetrating probes to determine the                          orientations of the proteins in the membrane.                                                                                                                        The proposed work is important to understand the mechanism                 by which plants generate the energy and metabolites necessary to                begin the germination process.  Past work discovered a new energy               shuttling mechanism within glyoxysomes.  The proposal work will                 determine its stucture and specific operation within the cell                   compartment.//
{'FirstName': 'Robert', 'LastName': 'Donaldson', 'PI_MID_INIT': 'P', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Robert P Donaldson', 'EmailAddress': 'robdon@gwu.edu', 'NSF_ID': '000374625', 'StartDate': '01/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'George Washington University', 'CityName': 'WASHINGTON', 'ZipCode': '200520042', 'PhoneNumber': '2029940728', 'StreetAddress': '1918 F ST NW', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'District of Columbia', 'StateCode': 'DC', 'CONGRESSDISTRICT': '00', 'CONGRESS_DISTRICT_ORG': 'DC00', 'ORG_UEI_NUM': 'ECR5E2LU5BL6', 'ORG_LGL_BUS_NAME': 'GEORGE WASHINGTON UNIVERSITY (THE)', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '1168', 'Text': None}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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