Determinants of Membrane Protein Topology and Structure
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
We will analyze the features of membrane proteins which determine               their arrangement within the lipid bilayer.  We will use fusions                of the periplasmic protein, alkaline phosphatase (AP), to the                   integral E. coli membrane protein, MalF.  Our results so far                    show that such fusions allow a reading-out of the information                   within a membrane protein that is important for its topology.  We               will use oligonucleotide-directed mutagenesis to alter sequences                within such fusions and examine the effect on topology.                                                                                                         Specific features of the protein which will be examined for their               importance in determining topology are 1) the charged amino acids               within cytoplasmic or periplasmic domains; 2) the charge                        distribution between periplasmic and cytoplasmic domains                        surrounding a membrane-spanning segment; 3) the length of                       membrane-spanning segments; and 4) the possible interaction                     between membrane-spanning segments.
[{'FirstName': 'Jonathan', 'LastName': 'Beckwith', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Jonathan Beckwith', 'EmailAddress': None, 'NSF_ID': '000108160', 'StartDate': '01/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Dana', 'LastName': 'Boyd', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Dana H Boyd', 'EmailAddress': 'dboyd@beck2.med.harvard.edu', 'NSF_ID': '000100463', 'StartDate': '01/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'Harvard University', 'CityName': 'CAMBRIDGE', 'ZipCode': '021385366', 'PhoneNumber': '6174955501', 'StreetAddress': '1033 MASSACHUSETTS AVE STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'LN53LCFJFL45', 'ORG_LGL_BUS_NAME': 'PRESIDENT AND FELLOWS OF HARVARD COLLEGE', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1112', 'Text': 'Genetic Mechanisms'}
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