Analysis of a Genetic Selection for Bacterial Evolution
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
The objective of this work is to understand the events that take                place during a non-lethal selection for mutation that increase                  the cells metabolic capability.  Specifically, we will study a                  selection for alterations which allow the uptake of large                       maltodextrins across the outer membrane of the bacterium                        Escherichia coli.  We will determine what parameters influence                  the selection and if the selection itself influences the type of                mutations that we obtain.  We will biochemically and genetically                characterize the events that occur during the long period of                    stationary growth on solid media that occurs during non-lethal                  selections.  We will determine if the selection results in                      induction of stress response systems.  And we will determine if                 starvation stress induces mutagenesis.  Finally, we will attempt                to determine the basis for the selection biases that occur.  The                knowledge gained from this work will help to clarify the nature                 of the events and forces that influence how cells respond to non-               lethal selections for enhanced growth ability.
{'FirstName': 'Spencer', 'LastName': 'Benson', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Spencer A Benson', 'EmailAddress': 'sbenson@umd.edu', 'NSF_ID': '000088799', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Maryland, College Park', 'CityName': 'COLLEGE PARK', 'ZipCode': '207425100', 'PhoneNumber': '3014056269', 'StreetAddress': '3112 LEE BUILDING', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Maryland', 'StateCode': 'MD', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'MD04', 'ORG_UEI_NUM': 'NPU8ULVAAS23', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF MARYLAND, COLLEGE PARK', 'ORG_PRNT_UEI_NUM': 'NPU8ULVAAS23'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8817208.xml'}