In situ Analyses of the Distributions and Phylogeny of      Cultivatable and Non-cultivatable Planctomycetales Using    Phylogenetic Group-Specific RNA Probes
{'Code': '08010208', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DEB', 'LongName': 'Division Of Environmental Biology'}}
{'SignBlockName': 'James R. Gosz', 'PO_EMAI': None, 'PO_PHON': None}
The majority of directly countable bacteria in microbial                   ecosystems cannot be identified or cultivated using traditional                 microbiological techniques thus, cultured bacteria may not be                   representative of actual microbial diversity.  The                              Planctomycetales are one of the ten major eubacterial divisions                 defined by 16S ribosomal RNA.  the unusual ribosomal RNA                        signatures of this group make them exceptionally well-suited for                microbial ecology studies using ribosomal RNA probe technologies.                    The recent development of phylogenetic group-specific DNA                  probes has made it possible to study the distribution and                       diversity of the Planctomycetales in natural habitats without the               biases of cultivation requirements.  Two probes for the                         Planctomycetales (PL1R and PL2R) and a "universal" rRNA probe                   will be used to measure the fraction of Planctomycetales                        ribosomal RNA genes in DNA extracted from mixed microbial                       populations.  Flourescently labeled DNA probes will be used to                  measure the contribution of the Planctomycetales to attached                    microbial populations adhering to contact slides.  Phylogenetic                 group-specific gene amplification primers will be used to                       identify particular constituents of the Planctomycetales                        population by the cloning and sequencing of rRNA genes.  These                  results will be compared to a data base of known Planctomycetales               sequences to determine whether previously uncultivated species                  are present in the population.
{'FirstName': 'Stephen', 'LastName': 'Giovannoni', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Stephen J Giovannoni', 'EmailAddress': 'stephen.giovannoni@oregonstate.edu', 'NSF_ID': '000466157', 'StartDate': '01/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Oregon State University', 'CityName': 'CORVALLIS', 'ZipCode': '973318655', 'PhoneNumber': '5417374933', 'StreetAddress': '1500 SW JEFFERSON AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Oregon', 'StateCode': 'OR', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'OR04', 'ORG_UEI_NUM': 'MZ4DYXE1SL98', 'ORG_LGL_BUS_NAME': 'OREGON STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1128', 'Text': 'Population & Community Ecology'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8818167.xml'}