Spirochete Genetics: Structure and Function of Linear       Plasmids of Borrelia
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
Little is known of the genetics of spirochetes, an ancient                      eubacterial division.  In the course of studies of the                          spirochetal agents of relapsing fever and Lyme disease, Dr.                     Barbour found that the members of the genus Borrelia have                       novel genetic elements, linear plasmids.  These linear                          plasmids bear the genes for the major surface proteins of the                   two species examined in most depth, B. hermsii and B.                           burgdorferi.  In possessing covalently-closed ends, the                         Borrelia plasmids resemble poxviruses and some eukaryotic                       replicons.  The specific aims of this project are the                           following: (l)  characterization of the structure and                           function of the linear plasmids, and (2) development of                         systems for genetic manipulation of spirochetes.                                                                                                                The project will wed capability in handling these difficult-                    to-handle and fastidious organisms with experience in the                       techniques of molecular genetics.  It is anticipated that the                   studies outlined in this proposal will reveal much about the                    genetics of spirochetes in general as well as the molecular                     biology of linear plasmids specifically.
{'FirstName': 'Alan', 'LastName': 'Barbour', 'PI_MID_INIT': 'G', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Alan G Barbour', 'EmailAddress': '5125673938', 'NSF_ID': '000444262', 'StartDate': '09/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Texas Health Science Center San Antonio', 'CityName': 'SAN ANTONIO', 'ZipCode': '782293901', 'PhoneNumber': '2105672340', 'StreetAddress': '7703 FLOYD CURL DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '20', 'CONGRESS_DISTRICT_ORG': 'TX20', 'ORG_UEI_NUM': 'C3KXNLTAAY98', 'ORG_LGL_BUS_NAME': 'THE UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '111200', 'Text': 'Genetic Mechanisms'}
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