Glycoforms of Phaseolin-Structure, Targeting and Stability
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
We propose to map the protein determinants which are likely                to be responsible for target specification and sorting of the                   seed storage protein phaseolin.  This will be approached by                     producing deletions in the coding sequence of a cloned phaseolin                gene and by constructing phaseolin/GUS gene chimeras, which will                be subsequently transferred into tobacco by using Ti plasmid-                   mediated transformation techniques.  Analysis of the expression                 and localization of the different constructs in transgenic seeds                will then be performed, to establish a correlation between                      specific peptide sequences and targeting properties.  N-linked                  glycosylation and sorting of phaseolin, will also be studied in                 an insect cell line infected with a baculovirus expression                      vector.                                                                            We intend to eventually be able to relate the function of                    specific sequences on the polypeptide portion of the molecule and               of oligosaccharides, to their structure.  In studies contemplated               in a separate proposal, the tertiary structure of the phaseolin                 protomers will be determined by X-ray crystallography.
{'FirstName': 'Timothy', 'LastName': 'Hall', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Timothy C Hall', 'EmailAddress': 'tim@idmb.tamu.edu', 'NSF_ID': '000381376', 'StartDate': '09/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Texas A&M Research Foundation', 'CityName': 'COLLEGE STATION', 'ZipCode': '778454375', 'PhoneNumber': '9798626777', 'StreetAddress': '400 HARVEY MITCHELL PKWY S STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'TX10', 'ORG_UEI_NUM': 'EQH8NQ4AXFT7', 'ORG_LGL_BUS_NAME': 'TEXAS A & M RESEARCH FOUNDATION', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '111400', 'Text': 'Cellular Dynamics and Function'}
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