T-Cell Development: Studies In Vivo and in Organ Culture
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Eve Ida Barak', 'PO_EMAI': None, 'PO_PHON': None}
The recent discovery of the T-cell receptor has generated                  enormous interest in T-cell development and function.  In an                    effort to study T-cell differentiation two cell types have been                 derived which appear to represent two stages of early thymocyte                 development:  1) a cell type that arises spontaneously under                    certain conditions in tissue culture, and 2) an immature T-cell                 line resulting from the Abelson virus (A-MuLV) infection of 13-14               day old thymocytes from C57Bl/6 mice.  In this study it is                      proposed to characterize in detail both cell lines, and to employ               techniques developed in this laboratory to infect early                         thymocytes with wild type A-MuLV to obtain T-cell lines stably                  transformed with a temperature sensitive mutant of Abelson virus                (ts-A-MuLV).  Immature thymocytes transformed with ts-A-MuLV and                shifted to the non-permissive temperature for v-abl (the Abelson                virus oncogene product) expression, under conditions suitable for               T-cell differentiation, may retain the capacity to proceed along                the normal pathway of T-cell differentiation.                                        This system may produce important information on the                       differentiation of T-cells previously unobtainable from T-cell                  lines "frozen" in one stage of development.
{'FirstName': 'Jerry', 'LastName': 'Guyden', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Jerry C Guyden', 'EmailAddress': 'jerry@scisun.sci.ccny.cuny.edu', 'NSF_ID': '000375736', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'CUNY City College', 'CityName': 'NEW YORK', 'ZipCode': '100319101', 'PhoneNumber': '2126505418', 'StreetAddress': '160 CONVENT AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '13', 'CONGRESS_DISTRICT_ORG': 'NY13', 'ORG_UEI_NUM': 'L952KGDMSLV5', 'ORG_LGL_BUS_NAME': 'RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '111400', 'Text': 'Cellular Dynamics and Function'}, {'Code': '111700', 'Text': 'CELLULAR PHYSIOLOGY'}, {'Code': '113600', 'Text': 'SIGNAL TRANSDCTN/CELL REGULATN'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '914500', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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