Photochemical Crosslinking Studies of Rat Epididymal Sperm  Plasma Membrane
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Eve Ida Barak', 'PO_EMAI': None, 'PO_PHON': None}
The objective of this study is to provide a comprehensive                  evaluation of polypeptides exposed to the hydrophilic (peripheral               proteins) or hydrophobic (integral proteins) region of the plasma               membrane of rat testicular and epididymal spermatozoa.  This will               be done by reacting intact spermatozoa recovered from rete testis               fluid and different regions of the epididymis with permeable,                   semi-permeable, or nonpermeable, radioactive chemical                           crosslinking reagents that are photosensitive.  Following                       photolysis the plasma membrane will be purified by density                      gradient centrifugation, and the proteins will be analyzed by                   polyacrylamide gel electrophoresis and autoradiography.  In                     other studies, nearest neighbor analysis of selected epididymal                 secreted proteins will be carried out.                                               By identifying the major polypeptides that comprise the                    integral and peripheral proteins of the sperm plasma membrane as                a function of epididymal transit, these studies may provide the                 groundwork for future investigations to test the functional                     significance of proteins that are added to, or removed from the                 sperm plasma membrane during development.  The function of                      surface proteins during fertilization also represents an                        important direction for further research.  Finally, these results               may prove important to our understanding of the role secreted                   proteins play in the reproductive process.
{'FirstName': 'Joe', 'LastName': 'Hall', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Joe C Hall', 'EmailAddress': None, 'NSF_ID': '000260848', 'StartDate': '09/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Pennsylvania State Univ University Park', 'CityName': 'UNIVERSITY PARK', 'ZipCode': '168021503', 'PhoneNumber': '8148651372', 'StreetAddress': '201 OLD MAIN', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '15', 'CONGRESS_DISTRICT_ORG': 'PA15', 'ORG_UEI_NUM': 'NPM2J7MSCF61', 'ORG_LGL_BUS_NAME': 'THE PENNSYLVANIA STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8714741.xml'}