"Role of Cellular Interactions in Sex Determination"
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith Plesset', 'PO_EMAI': None, 'PO_PHON': None}
In hydra, the somatic cells and gametes arise from a population                 of multipotent stem cells, interstitial cells (I-cells).                        Although this population was thought to be uniform, recent                      evidence suggests that there are subpopulations of I-cells with                 different developmental potentials.  One subpopulation has been                 isolated that is restricted to sperm differentiation. 97% of the                cells in this subpopulation label with a monoclonal antibody,                   AC2, which is specific for cells of the spermatogenic pathway.                  The 3% that do not bind the antibody are thought to be the                      precursors to the AC2-binding cells. The importance of this                     subpopulation in determining sex has been demonstrated during the               previous award. The focus of this study will be: 1) to determine                if AC2- sperm precursor cells are the only source of the AC2-                   binding cells. 2) to determine where in the egg lineage pathway                 suppression of egg production occurs in the presence of male-                   derived I-cells. This requires isolating markers specific for                   cells of the egg pathway. 3) to determine if the same I-cell that               produces eggs in a female can be induced to make sperm in a male.               Finally, 4) to begin looking for a molecular basis for the                      suppression of egg formation by male I-cells. This will involve                 producing cDNA libraries containing male or female specific                     sequences.                                                                      %%%                                                                             The main objective of this work is to elucidate the extent to                   which cell-cell  and cell-environment interactions control the                  determination of sex during development. Dr. Littlefield will use               the simple organism Hydra as a model system to address these                    questions.
{'FirstName': 'Charlotte', 'LastName': 'Littlefield', 'PI_MID_INIT': 'L', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Charlotte L Littlefield', 'EmailAddress': None, 'NSF_ID': '000472361', 'StartDate': '02/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of California-Irvine', 'CityName': 'IRVINE', 'ZipCode': '926970001', 'PhoneNumber': '9498247295', 'StreetAddress': '160 ALDRICH HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '47', 'CONGRESS_DISTRICT_ORG': 'CA47', 'ORG_UEI_NUM': 'MJC5FCYQTPE6', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF CALIFORNIA IRVINE', 'ORG_PRNT_UEI_NUM': 'MJC5FCYQTPE6'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '111100', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8819247.xml'}