Growth, Product Formation and Cell Differentiation in Plant Cell Biofilms
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
This proposal seeks to study:                                                                                                                                   l) The growth and product formation kinetics of plant cell biofilms             grown on inert support materials.  A mathematical model is to be                formulated and tested which describes film growth and secondary                 metabolite formation by plant cells with and without substrate diffusion        limitations.                                                                                                                                                    2) The influence of biofilm thickness and micro-environmental conditions        within the film on cell differentiation and secondary metabolite                formation.                                                                                                                                                      The plant Catharanthus roseus and its secondary metabolite, ajmalicine          (a pharmaceutical used to treat blood circulation problems), has been           selected as a model system for this study.  The proposed use of plant           cell biofilm cultures is new and unique and may overcome the problems of        cell-cell contact and control of the micro-environmental conditions             thought to be necessary for maintaining cell differentiation and the            hyothesized higher product yields.  It is suggested that the proposed           novel culturing technique could result in a better culturing technique          for other types of plant cells.                                                                                                                                 The Principal Investigator is considered to be wall qualified to carry          out the proposed research and I recommend funding of this proposal for          two years in accordance with the submitted budget.
{'FirstName': 'Fikret', 'LastName': 'Kargi', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Fikret Kargi', 'EmailAddress': None, 'NSF_ID': '000180155', 'StartDate': '01/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Washington University', 'CityName': 'SAINT LOUIS', 'ZipCode': '63110', 'PhoneNumber': '3147474134', 'StreetAddress': 'ONE BROOKINGS DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Missouri', 'StateCode': 'MO', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'MO01', 'ORG_UEI_NUM': 'L6NFUM28LQM5', 'ORG_LGL_BUS_NAME': 'WASHINGTON UNIVERSITY, THE', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8612936.xml'}