Protein Refolding in the Activation of Denatured Xylose     Isomerase
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Maria Burka', 'PO_EMAI': None, 'PO_PHON': None}
A large percentage of protein products from genetically engineered              cells exist in scrambled forms and are biologically nonfunctional.              First, unfolding and then refolding these inactive products into active         proteins is one of the most important problems of the new                       biotechnology.  Hardly any bioprocess engineering research has ever             been done in this area and, in fact, most engineers have not been aware         of such difficulties until recently.  This study will examine several           related approaches to refold a model protein, xylose isomerase, after           first unfolding.  The objective is the development of bioprocessing             techniques that will allow improved percentage yield of active products         from scrambled proteins.
{'FirstName': 'George', 'LastName': 'Tsao', 'PI_MID_INIT': 'T', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'George T Tsao', 'EmailAddress': 'tsaogt2@yahoo.com', 'NSF_ID': '000438069', 'StartDate': '08/15/1986', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Purdue University', 'CityName': 'WEST LAFAYETTE', 'ZipCode': '479061332', 'PhoneNumber': '7654941055', 'StreetAddress': '2550 NORTHWESTERN AVE # 1100', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Indiana', 'StateCode': 'IN', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'IN04', 'ORG_UEI_NUM': 'YRXVL4JYCEF5', 'ORG_LGL_BUS_NAME': 'PURDUE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1402', 'Text': 'BIOCHEMICAL & BIOMASS ENG'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8610844.xml'}