Hydrate Formation and Protein Solubilization in Reversed    Micelles (Expedited Award)
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Robert M. Wellek', 'PO_EMAI': None, 'PO_PHON': None}
This expedited award project is a study on protein solubilization               characteristics in reversed micelles under conditions where the water           pools within the micelles are partially converted to clathrate                  hydrates.  Hydrate formation in reversed micelles may be a novel and            viable method to control the solubilization of proteins within the              micelles.  The present technique to extract proteins from fermentation          broths into reversed micelles and subsequently recover them from the            micelles involves cycling the ionic strength, promoting solubilization          with low ionic strenth, high ionic strength causing desolubilization.           Such approaches can weaken enzymes.  The extraction approach under              investigation is to solubilize protein in reversed micelles at low              ionic strength, and ten to induce the formation of clathrate hydrates           under relatively low temperatures (273-283 K) and moderate pressures            (0.2-5 MPa) to effect protein recovery out of the micelles.  Formation          of hydrates, which are ice-like structures, would effectively reduce            the microaqueous phase volume within the micelles, and thus control             desolubilization characteristics.                                                                                                                               The exploratory research project is divided into three parts (a) the            effect of moderately enhanced pressures on protein solubilization in            reversed micelles under ambient temperature conditions where hydrate            formation is prevented (b) the study of hydrate formation in reversed           micelles at low temperatures, and comparisons with hydrate formation            in bulk aqueous media, and most importantly, the synthesis of parts             (a) and (b), i.e. (c) a study of the effects of hydrate formation on            protein solubilization in reversed micelles.
{'FirstName': 'Vijay', 'LastName': 'John', 'PI_MID_INIT': 'T', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Vijay T John', 'EmailAddress': 'vijay.john@tulane.edu', 'NSF_ID': '000206852', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Tulane University', 'CityName': 'NEW ORLEANS', 'ZipCode': '701185665', 'PhoneNumber': '5048654000', 'StreetAddress': '6823 SAINT CHARLES AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Louisiana', 'StateCode': 'LA', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'LA01', 'ORG_UEI_NUM': 'XNY5ULPU8EN6', 'ORG_LGL_BUS_NAME': 'ADMINISTRATORS OF THE TULANE EDUCATIONAL FUND, THE', 'ORG_PRNT_UEI_NUM': 'XNY5ULPU8EN6'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '141400', 'Text': 'INTERFAC PROCESSES & THERMODYN'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8802564.xml'}