The Role of Myosin Cross-Bridge Orientation in Muscle       Contraction
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Arthur Kowalsky', 'PO_EMAI': None, 'PO_PHON': None}
The aim of this project is to elucidate the molecular                           mechanism of muscle contraction by following the rotational                     motion of specific probes attached to myosin cross-bridges                      in muscle fibers.  Muscle fibers will be perturbed chemically                   or mechanically and the effect of these perturbations on the                    orientation of the cross-bridges will be followed in the                        steady state and time-resolved modes.  The resulting data                       will be correlated with the rotating cross-bridge model of                      muscle contraction.                                                                                                                                             An appealing model of the molecular mechanism of muscle                         contraction proposes that active protein elements of the                        mechanism move in a cyclical manner in synchrony with the                       hydrolysis of ATP to produce muscle shortening against a                        load.  It is known that the head region of myosin (the                          cross-bridge) contains the hydrolysis site for ATP and acts                     as a transducer converting chemical energy to mechanical                        energy.  Actin is also of interest in this class of models                      since the actin filament has specific binding sites for                         myosin.  A popular model of model contraction, the rotating                     cross-bridge model, serves as a focal point of the                              experimental investigation of muscle contrating mechanisms by                   Dr. Burghardt.
{'FirstName': 'Thomas', 'LastName': 'Burghardt', 'PI_MID_INIT': 'P', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Thomas P Burghardt', 'EmailAddress': 'burghardt@mayo.edu', 'NSF_ID': '000318229', 'StartDate': '09/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Mayo Clinic Rochester', 'CityName': 'ROCHESTER', 'ZipCode': '559050001', 'PhoneNumber': '5072844715', 'StreetAddress': '200 1ST ST SW', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'MN01', 'ORG_UEI_NUM': 'Y2K4F9RPRRG7', 'ORG_LGL_BUS_NAME': 'MAYO CLINIC', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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