Development of Neuron-to-Neuron Synapses in Vitro
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Christopher Platt', 'PO_EMAI': None, 'PO_PHON': None}
This research will focus on development of neuron-to-neuron                     synapses and will be aimed at testing the validity of                           neuromuscular junctions as a model for neuron-to-neuron synapses.               Specifically, motoneurons will be isolated from embryonic or                    young post-natal rats, using a flourescence-activated cell                      sorter, and will be grown in culture with neurons that should and               neurons that should not form synapses onto them.  The presence of               synapses will be ascertained electrophysically and                              immunocytochemically.  The numbers and subcellular distributions                of two types of neurotransmitter receptors (those for a peptide,                substance P, and an amino acid, glycine) will be monitored, and                 the effects of the presence of other neurons and of formation of                synapses will be determined.  Particular attention will be given                to whether inducers of different types of receptors acting on the               same neuron combine in a linearly additive or synergistic manner.               A working hypothesis for this work is that changes in subcellular               distributions of receptors will be more prominent in neuron-to-                 neuron synaptogenesis than will effects on total receptor number.
{'FirstName': 'Paul', 'LastName': 'St. John', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Paul A St. John', 'EmailAddress': 'stjohn@email.arizona.edu', 'NSF_ID': '000099041', 'StartDate': '08/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Arizona', 'CityName': 'TUCSON', 'ZipCode': '85721', 'PhoneNumber': '5206266000', 'StreetAddress': '845 N PARK AVE RM 538', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Arizona', 'StateCode': 'AZ', 'CONGRESSDISTRICT': '07', 'CONGRESS_DISTRICT_ORG': 'AZ07', 'ORG_UEI_NUM': 'ED44Y3W6P7B9', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF ARIZONA', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '119000', 'Text': 'DEVELOPMENTAL NEUROSCIENCE'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8808506.xml'}