Mechanisms of Light-Clock Interaction in Plant Cells
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Kamal Shukla', 'PO_EMAI': None, 'PO_PHON': None}
Ion transport and leaflet movement will be studied with:                                                                                                             1) Single and double barrel ion-sensitive microelectrodes                          to measure the dynamic relationship of H+, K+, Cl- and                          Ca++ fluxes in the pulvinar apoplast, in response to                            light, channel blockers, and pump inhibitors; and                                                                                                            2) Patch clamp methods to probe for changes in the                                 electrical behavior of ion pumps and channels in                                Samanea protoplast membranes in response to light,                              channel blockers, and a variety of ionic conditions:                                                                                                    To further probe the mechanism of light signal transduction,                    standard biochemical methods will be used to elucidate the                      diacylglyerol portion of the PI pathway.  The effects of PI cycle                                                                                               intermediates on ion transport will also be investigated.%%%                                                                                                    Samanea saman is a nyctinastic legume with paired leaflets that                 usually separate from each other (open) in the light and fold                   together (close) in the dark.  These leaf movements are also                    under circadian control and furnish a convenient model system for                                                                                               studying biological time measurements in plants.  They are turgor                                                                                               regulated and driven by movements of ions through membranes of                                                                                                  the cells in the motor organ (pulvinus).  The ion movements                                                                                                     result from rhythmic and light-regulated changes in pumps and                                                                                                   channels in the membranes.  The light signals require                                                                                                           amplification and the plant uses the phosphatidylinositol (PI)                                                                                                  pathway.  This pathway is well studied in animals but unexplored                                                                                                in plants.  This project will study the tranduction mechanism                                                                                                   using a variety of techniques.***//
{'FirstName': 'Donald', 'LastName': 'Wetherell', 'PI_MID_INIT': 'F', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Donald F Wetherell', 'EmailAddress': None, 'NSF_ID': '000111981', 'StartDate': '07/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Connecticut', 'CityName': 'STORRS', 'ZipCode': '062699018', 'PhoneNumber': '8604863622', 'StreetAddress': '438 WHITNEY RD EXTENSION UNIT 11', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Connecticut', 'StateCode': 'CT', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'CT02', 'ORG_UEI_NUM': 'WNTPS995QBM7', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF CONNECTICUT', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '114400', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8804810.xml'}