Protein Sequencer for Structural Analysis of Proteins
{'Code': '08080102', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DBI', 'LongName': 'Div Of Biological Infrastructure'}}
{'SignBlockName': 'Kathie L. Olsen'}
Determination of the sequence of amino acids is the first step                  toward the study of cloning and structure-function relationships                of proteins.  DNA of known base sequence can be synthesized if                  the amino acid sequence of a protein is known.  Genes for several               proteins are proposed to be cloned in the Biochemistry                          Department; these include actin depolymerization factor and an                  rRNA transcription initiation factor.  Amino acid sequence                      is also essential to find the site of proteins damaged by                       oxyradicals and hydrogen peroxide; for the region of                            neurotoxin-acetylcholine receptor binding; to elucidate the                     active center of snake hemorrhagic toxins; and to determine the                 regulatory site of phosphorylation in actin depolymerizing                      factor.  The active sites of the RNA polymerases from E. coli and               bacteriophage T7 are also proposed to be studied.                                                                                                               Unfortunately, there is no protein sequencer at Colorado State                  University.  At the moment we are relying on a facility at a                    neighboring state university.  Our ability to do protein research               is impaired by the lack of more direct and timely access to this                instrumentation.  Acquisition of a sequenator will enhance the                  efficiency and quality of protein research at Colorado State                    University.
[{'FirstName': 'Anthony', 'LastName': 'Tu', 'EmailAddress': None, 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Robert', 'LastName': 'Woody', 'EmailAddress': 'rww@lamar.colostate.edu', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Winslow', 'LastName': 'Caughey', 'EmailAddress': None, 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'James', 'LastName': 'Bamburg', 'EmailAddress': 'james.bamburg@colostate.edu', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Marvin', 'LastName': 'Paule', 'EmailAddress': None, 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Colorado State University', 'CityName': 'Fort Collins', 'ZipCode': '805232002', 'PhoneNumber': '9704916355', 'StreetAddress': '601 S Howes St', 'CountryName': 'United States', 'StateName': 'Colorado', 'StateCode': 'CO'}
{'Code': '1108', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8820626.xml'}