Quantitative Fluorescence Microscopy by Digital Image       Analysis
{'Code': '08000000', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}}
{'SignBlockName': 'name not available', 'PO_EMAI': None, 'PO_PHON': None}
A video image memory system, acquistion procesor and digitizer, and a           cursor generator will be acquired to complete a distributed, yet                integrated, imaging facilty for the Center for Fluorescence Research in         Biomedical Sciences.  Interactive video image acquisition and analysis,         and supermini computers for data acquisition and reduction are also part        of the imaging facility.  Four separate laboratories are networked to           allow multiple user applications.  A wide variety of projects in cell           biology, cell physiology, developmental biology, regulatory biology, and        biological chemistry will be supported by in the instrumentation.  Among        the projects to be supported are analyses of the growth factor                  stimulation of quiescent cells and macrophage chemotaxis.  Ratio imaging        will be used as a spectroscopic method for determining the spatial and          temporal changes in (cellular) physiological parameters such as pH and          pCa.                                                                                                                                                            Multiple parameter analysis of two or more separable fluorescent probes         will also be used to correlate spatial and temporal dynamics of a               variety of cellular funcitons.  Three dimensional reconstruction will be        carried out on actin networks in living cells.  A method of two                 wavelength total internal reflection image analysis will be used to             quantitate the distance from the cell substrate to selected molecules.          Methods for mapping the spatial and temporal variations in fluorescence         lifetime and fluorescence anisotropy of suitably labeled proteins in            living cells will be developed.  The first target for mapping with be           calmodulin.  Overall, the equipment, coupled with other resources in the        Center, will permit state-of-the-art measurements greatly extending our         understanding of cellular function.
[{'FirstName': 'Robert', 'LastName': 'Birge', 'PI_MID_INIT': 'R', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Robert R Birge', 'EmailAddress': 'rbirge@uconn.edu', 'NSF_ID': '000154923', 'StartDate': '05/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Alan', 'LastName': 'Waggoner', 'PI_MID_INIT': 'S', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Alan S Waggoner', 'EmailAddress': None, 'NSF_ID': '000418096', 'StartDate': '05/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'D. Lansing', 'LastName': 'Taylor', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'D. Lansing Taylor', 'EmailAddress': None, 'NSF_ID': '000230023', 'StartDate': '05/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Frederick', 'LastName': 'Lanni', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Frederick Lanni', 'EmailAddress': 'lanni@cmu.edu', 'NSF_ID': '000193532', 'StartDate': '05/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Carnegie-Mellon University', 'CityName': 'PITTSBURGH', 'ZipCode': '152133815', 'PhoneNumber': '4122688746', 'StreetAddress': '5000 FORBES AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '18', 'CONGRESS_DISTRICT_ORG': 'PA18', 'ORG_UEI_NUM': 'U3NKNFLNQ613', 'ORG_LGL_BUS_NAME': 'CARNEGIE MELLON UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'U3NKNFLNQ613'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8609687.xml'}