Structural Basis of Transfer Ribonucleic Acid Function
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Arthur Kowalsky', 'PO_EMAI': None, 'PO_PHON': None}
The nature and rate of conformational changes that take place                   in transfer RNA (tRNA) as it interacts with the                                 macromolecular components involved in protein synthesis                         remain unclear.  Nuclear magnetic resonance (NMR) is a                          powerful, non-destructive method for studying the structure,                    conformation and dynamic properties of tRNA in solution.                        While most NMR studies have involved protein NMR, F-19 NMR                      offers the advantages of high sensitivity to detection, a                       large range of chemical shifts and great sensitivity to the                     environment of the nucleus.                                                                                                                                     Dr. Horowitz is using high resolution F-19 NMR to probe                         conformational changes in  5-fluorouracil (FUra)-substituted                    E. coli tRNAs as they relate to function.  Interpretation of                    results at the molecular level requires reliable peak                           assignments; approximately half the resonances in the F-19                      NMR spectrum of the valine tRNA have been assigned.  This                       project will be expanded to include experiments with                            molecular fragments and hybrid tRNA molecules, chemical                         modification studies and the investigation of tRNAs with                        specific FUra residues replaced with other bases by                             recombinant DNA techniques.  Then will be used in                               assigning the remaining peaks in the F-19 spectrum.
{'FirstName': 'Jack', 'LastName': 'Horowitz', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Jack Horowitz', 'EmailAddress': None, 'NSF_ID': '000185639', 'StartDate': '07/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Iowa State University', 'CityName': 'AMES', 'ZipCode': '500112103', 'PhoneNumber': '5152945225', 'StreetAddress': '1350 BEARDSHEAR HALL', 'StreetAddress2': '515 MORRILL ROAD', 'CountryName': 'United States', 'StateName': 'Iowa', 'StateCode': 'IA', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'IA04', 'ORG_UEI_NUM': 'DQDBM7FGJPC5', 'ORG_LGL_BUS_NAME': 'IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8704978.xml'}