Chemistry Structure and Function of the Non-Tubulin Spindle Matrix in Dividing Cells
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
This proposal deals with the study of the mitotic spindles of                   invertebrates zygotes.  The major goal is to purify tektin-like proteins        from mitotic spindles and to determine how these proteins interact with         the microtubules of the spindle and the spindle matrix.  Tektin                 antibodies will be used to identify and purify similar proteins from the        spindles and these proteins will be used to generate antibodies to be           used as specific probes.  Laser confocal light microscopy and                   transmission and scanning electron microscopy will be used to determine         the 3-dimensional organization of these matrix polypeptides.  Antibodies        will be microinjected into living cells to determine effects on spindle         structure and function.                                                                                                                                         These studies will advance our understanding of microtubule assembly,           polarity and stability in the mitotic spindle and how the matrix                provides a framework for the operation of the cell division process.
{'FirstName': 'Richard', 'LastName': 'Linck', 'PI_MID_INIT': 'W', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Richard W Linck', 'EmailAddress': 'linck001@umn.edu', 'NSF_ID': '000447656', 'StartDate': '02/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Minnesota-Twin Cities', 'CityName': 'MINNEAPOLIS', 'ZipCode': '554552009', 'PhoneNumber': '6126245599', 'StreetAddress': '200 OAK ST SE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MN05', 'ORG_UEI_NUM': 'KABJZBBJ4B54', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF MINNESOTA', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8811015.xml'}