Optimization of Protein Overproduction by Recombinant       Microorganisms
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
Three undergraduate students are to be supported by this supplementary          request.  Their resumes are included with this request.  The proposed           work involves three undergraduate research projects which are as                follows:                                                                                                                                                        1.   Experimental Study of Plasmid Loss                                              The undergraduate student will run continuous fermentations with                bacteria containing plasmids encoded to overproduce a protein.                  Samples will be taken and analyzed in order to correlate                        plasmid loss with cell growth and protein production rate.                      Results will be compared with a mathematical model.                                                                                                        2.   Modeling and Control of Invertase Overproduction                                The undergraduate student will perform shake flask                              experiments and scale-up experiments with a 10-15 liter                         fermentor in order to test and further develop a mathematical                   model of the secretion of overproduced invertase by yeast.                                                                                                 3.   Selective Recycle via Flocculation and Sedimentation                            The undergraduate student will run a continuous recycle                         bioreactor configured to maintain a slower growing flocculent                   strain in spite of competition from a faster-growing non-                       flocculent strain.  He or she will also perform coflocculation                  studies with mixed cultures and measure the degree of separation                of the two strains that can be achieved by sedimentation in a                   inclined settler.                                                                                                                                          Each of the three undergraduate students will be supervised by and              meet at least biweekly with one of the co-PIs on the NSF grant.  In             addition, a graduate student supported by the NSF grant will be                 assigned as a mentor to each undergraduate student in order to assist           the undergraduate with the day-to-day aspects of carrying out each              project.
[{'FirstName': 'Dhinakar', 'LastName': 'Kompala', 'PI_MID_INIT': 'S', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Dhinakar S Kompala', 'EmailAddress': 'dhinakar.kompala@colorado.edu', 'NSF_ID': '000290114', 'StartDate': '03/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Robert', 'LastName': 'Sclafani', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Robert A Sclafani', 'EmailAddress': '3032707288', 'NSF_ID': '000160188', 'StartDate': '03/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Austin', 'LastName': 'Taylor', 'PI_MID_INIT': 'L', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Austin L Taylor', 'EmailAddress': None, 'NSF_ID': '000476271', 'StartDate': '03/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'W. Fred', 'LastName': 'Ramirez', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'W. Fred Ramirez', 'EmailAddress': 'fred.ramirez@colorado.edu', 'NSF_ID': '000358320', 'StartDate': '03/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Robert', 'LastName': 'Davis', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Robert H Davis', 'EmailAddress': 'robert.davis@colorado.edu', 'NSF_ID': '000466005', 'StartDate': '03/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'University of Colorado at Boulder', 'CityName': 'Boulder', 'ZipCode': '803090001', 'PhoneNumber': '3034926221', 'StreetAddress': '3100 MARINE ST', 'StreetAddress2': 'STE 481 572 UCB', 'CountryName': 'United States', 'StateName': 'Colorado', 'StateCode': 'CO', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'CO02', 'ORG_UEI_NUM': 'SPVKK1RC2MZ3', 'ORG_LGL_BUS_NAME': 'THE REGENTS OF THE UNIVERSITY OF COLORADO', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '149100', 'Text': 'Cellular & Biochem Engineering'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8842370.xml'}