Genetic Engineering of Proteins to Enhance Product Recovery
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
Using molecular biology to bestow a characteristic on a product                 that permits its selective removal from a multicomponent cellular               extract can be of immense benefit in the downstream processing of               the products of biotechnology, including enzymes, hormones and                  antibodies.  Theory and past results both indicate that the                     addition of charged tails to proteins can aid the selectivity of                separation methods.                                                                                                                                             This proposal seeks to fuse polypeptide tails to proteins in                    order to enhance the recovery of proteins.  Beta-galactosidase                  has been chosen as a model protein to carry out this proposal.                  The work plan includes the following:                                                  1.  Design of the polypeptide tail.                                             2.  Synthesis of the gene segment for the tail.                                 3.  Cloning this segment into the beta-galactosidase gene.                      4.  Expression of the gene in E. coli.                                          5.  Assessment of structural and/or activity changes in                                the target protein.                                                      6.  Recovery of the fused protein by precipitation and                              two-phase aqueous extraction.                                                                                                                        This grant application is an excellent example of the type of                   cross-disciplinary proposal we wish to see in the Engineering                   Directorate's Biotechnology Program.  The Principal Investigators               are considered to be well qualified to carry out the proposed                   research.
[{'FirstName': 'Steven', 'LastName': 'Gendel', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Steven M Gendel', 'EmailAddress': None, 'NSF_ID': '000273694', 'StartDate': '11/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Malcolm', 'LastName': 'Rougvie', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Malcolm A Rougvie', 'EmailAddress': None, 'NSF_ID': '000096295', 'StartDate': '11/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Clark', 'LastName': 'Ford', 'PI_MID_INIT': 'F', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Clark F Ford', 'EmailAddress': 'cfford@iastate.edu', 'NSF_ID': '000457803', 'StartDate': '11/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Charles', 'LastName': 'Glatz', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Charles E Glatz', 'EmailAddress': 'cglatz@iastate.edu', 'NSF_ID': '000128897', 'StartDate': '11/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'Iowa State University', 'CityName': 'AMES', 'ZipCode': '500112103', 'PhoneNumber': '5152945225', 'StreetAddress': '1350 BEARDSHEAR HALL', 'StreetAddress2': '515 MORRILL ROAD', 'CountryName': 'United States', 'StateName': 'Iowa', 'StateCode': 'IA', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'IA04', 'ORG_UEI_NUM': 'DQDBM7FGJPC5', 'ORG_LGL_BUS_NAME': 'IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
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