Analysis of a Novel Immobilized Cell Reactor for Unstable   Recombinant Cultures
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
This proposal seeks to develop an immobilized cell reactor in                   which a recombinant DNA instability problem (plasmid instability)               is overcome by selectively killing cells without recombinant DNA                (without a plasmid).  This is to be done using a toxin (colicin)                to E. coli cells.  A plasmid is to be used that confers                         resistance of these cells to colicin as well as a receptor site                 on the cell surface to this toxin.  Colicin is to be attached to                a column of sepharose beads and act as a binding agent for the E.               coli.  Those cells without the plasmid presumably will be killed                and wash off the column while those cells with the plasmid will                 presumably stay attached to the column.                                                                                                                         The advantage of the above system to a suspension cell culture                  (fermentation) is twofold.                                                                                                                                             -  The toxin is not in solution and will not wash out of                           the reactor thus saving on the cost of the toxin.                                                                                                            -  The product of the bioreactor will not need to pass                             through a purification step to eliminate the toxin.                                                                                                   The PI is considered to be well qualified to carry out the                      proposed research and I recommend funding of this proposal.
{'FirstName': 'Martin', 'LastName': 'Hjortso', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Martin A Hjortso', 'EmailAddress': 'hjortso@lsu.edu', 'NSF_ID': '000377373', 'StartDate': '06/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Louisiana State University', 'CityName': 'BATON ROUGE', 'ZipCode': '708030001', 'PhoneNumber': '2255782760', 'StreetAddress': '202 HIMES HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Louisiana', 'StateCode': 'LA', 'CONGRESSDISTRICT': '06', 'CONGRESS_DISTRICT_ORG': 'LA06', 'ORG_UEI_NUM': 'ECQEYCHRNKJ4', 'ORG_LGL_BUS_NAME': 'LOUISIANA STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'Z7ZNZH7LJ778'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8710512.xml'}