Cell Permeable Probes for Free Magnesium Ion
{'Code': '08060200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DCB', 'LongName': 'Division of Cellular Biosciences'}}
{'SignBlockName': 'Maryanna P. Henkart'}
This research is directed toward the development of cell permeable         metallochromic indicators for free magnesium ion.  Preliminary data             suggest that tropolone derivatives possess both the necessary                   selectivity for magnesium and against other ions and adequate absorbance        spectra for this use.  Current goals are to synthesize additional               derivatives of the tropolone compounds already  prepared to enhance             their absorbance and binding properties.  Potential magnesium sensitive         dyes will be tested in three cell systems chosen because of their               specific magnesium-related properties:  (1) Ehrlich ascites cells, for          which the intracellular free magnesium concentration has been                   determined, (2) Murine S49 lymphoma cells which have a hormone-sensitive        magnesium influx (but not efflux) system, and thus exhibit a hormone-           sensitive change in cell magnesium content, and (3) synchronized                cultures of Schizosaccharomyces pombe which exhibit a 5 to 10-fold              change in total cell magnesium content over a short, specific period in         the cell cycle.                                                                      Magnesium ion activates or inhibits a numer of important enzyme            systems, and magnesium transport appears to be regulated by a variety of        hormones.  Detailed studies of the possible role of free intracellular          magnesium in regulation of cellular functions have been severely                hampered, however, by the lack of a relatively simple, easily applied           method to determine the free intracellular magnesium ion concentration          on a physiologically relevant time scale.  This research, if successful,        will provide much needed probes for studies of the role of magnesium ion        in cellular physiology.
[{'FirstName': 'Robert', 'LastName': 'Salomon', 'EmailAddress': None, 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'George', 'LastName': 'Dubyak', 'EmailAddress': None, 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Michael', 'LastName': 'Maguire', 'EmailAddress': 'mem6@cwru.edu', 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'Case Western Reserve University', 'CityName': 'CLEVELAND', 'ZipCode': '441064901', 'PhoneNumber': '2163684510', 'StreetAddress': 'Nord Hall, Suite 615', 'CountryName': 'United States', 'StateName': 'Ohio', 'StateCode': 'OH'}
[{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}, {'Code': '1117', 'Text': 'CELLULAR PHYSIOLOGY'}, {'Code': '1950', 'Text': 'METHODOLOGY'}, {'Code': '1978', 'Text': 'PROJECTS'}, {'Code': '1982', 'Text': 'BIOLOGICAL CHEMISTRY'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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