Genetic Hypervariability in Halobacterium Halobium
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
The extremely halophilic archaebacterium, Halobacterium halobium,               has the capability to synthesize intracellular gas-filled                       vesicles for buoyancy to regulate the availability of light and                 oxygen.  The mutation rate for gas vesicle synthesis (Vac) is the               highest of several phenotypic hypervariabilities observed in H.                 halobium.  The Vac mutation rate (1%) is 100-fold higher than for               the well-characterized purple membrane mutations which are the                  result of insertional inactivation of the bacterio-opsin (bop                   gene (DasSarma, et al., unpublished), we are now in an ideal                    position to study the mutation, structure, and regulation of the                gvh gene using standard methods of molecular genetics.  We plan                 to:  (a) examine the physical and transcriptional state of the                  gvh gene region in many Vac mutants by Southern and Northern                    hybridizations; and (b) complete the DNA sequence of the gvh gene               region and determine the structure of the mRNA by mapping with S1               nuclease and capping enzyme.
{'FirstName': 'Shiladitya', 'LastName': 'DasSarma', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Shiladitya DasSarma', 'EmailAddress': 'sdassarma@som.umaryland.edu', 'NSF_ID': '000248545', 'StartDate': '07/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Massachusetts Amherst', 'CityName': 'AMHERST', 'ZipCode': '010039252', 'PhoneNumber': '4135450698', 'StreetAddress': '101 COMMONWEALTH AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'MA02', 'ORG_UEI_NUM': 'VGJHK59NMPK9', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF MASSACHUSETTS', 'ORG_PRNT_UEI_NUM': 'VGJHK59NMPK9'}
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[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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