Substrate and Primer Binding Sites in Terminal              Deoxynucleotidyl Transferase
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
He has used TdT as a model DNA polymerase to analyze the                   process of substrate and primer binding.  Several site-specific                 reagents and procedures for the site-specific covalent                          modifications have been developed to label the desired site(s)                  in TdT.  Using an affinity labeling protocol, we have                           demonstrated the identity of the substrate binding domain in                    the 3 molecular weight species of TdT.  He will now define the                  primary amino acid sequence of that domain as well as identify                  the amino acid residue which participates in binding of the                     substrate triphosphate.  Studies are also planned to define the                 primer binding site domain in TdT which utilizes a) covalent                    crosslinking of defined length oligomeric DNA primer to various                 MW species of TdT and b) covalent chemical linkage of Ap5A, a                   newly found inhibitor, to the primer binding site.                                   An analysis of peptides involved in primer binding and                     their primary amino acid sequence together with investigation                   on the mechanism of inhibition of some Ap5A analogues is                        expected to provide a general geometry of the active site                       structure involved in substrate and primer binding in TdT.
{'FirstName': 'Mukund', 'LastName': 'Modak', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Mukund J Modak', 'EmailAddress': 'modak@umdnj.edu', 'PI_PHON': '9739725515', 'NSF_ID': '000153073', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'New Jersey Medical School', 'CityName': 'Newark', 'ZipCode': '071032407', 'PhoneNumber': None, 'StreetAddress': '100 Bergen Street', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New Jersey', 'StateCode': 'NJ', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'NJ10', 'ORG_UEI_NUM': None, 'ORG_LGL_BUS_NAME': None, 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1144', 'Text': 'Molecular Biophysics'}
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