Structural Studies of Bacterial Luciferase:  Subunit        Folding and Dimer Assembly in Vivo
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
How a newly translated linear polypeptide folds into a                     functional native protein is the central issue in protein                       biochemistry today.  The purpose of the proposed research is to                 delineate the mechanism by which the subunits of bacterial                      luciferase fold and assemble into the functional heterodimeric                  enzyme in vivo.  Genes for the two subunits, luxA and luxB,                     have been cloned and expressed in E. coli.   Thus, the subunits                 can be easily prepared.  Moreover, their structures can be                      readily altered by oligonucleotide-directed site specific                       mutagenesis.  The ease with which low light intensities can be                  measured permits the detection of specific activites in mutants                 over a range of six orders of magnitude.  Thus, the luciferase                  heterodimer provides an ideal system for studying the                           principles which govern protein folding in vivo.  In this                       project mutants of the subunits will be prepared, and the way                   they combine to form active enzyme will be analyzed through                     kinetic and physical characterization of the folding and                        assembly process.
{'FirstName': 'Thomas', 'LastName': 'Baldwin', 'PI_MID_INIT': 'O', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Thomas O Baldwin', 'EmailAddress': 'tbaldwin@ucr.edu', 'NSF_ID': '000366880', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Texas A&M Research Foundation', 'CityName': 'COLLEGE STATION', 'ZipCode': '778454375', 'PhoneNumber': '9798626777', 'StreetAddress': '400 HARVEY MITCHELL PKWY S STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'TX10', 'ORG_UEI_NUM': 'EQH8NQ4AXFT7', 'ORG_LGL_BUS_NAME': 'TEXAS A & M RESEARCH FOUNDATION', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '114400', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8716262.xml'}