Metabolic Mapping with (14C)-2-Deoxyglucose and (14C)-      Glucose
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'name not available', 'PO_EMAI': None, 'PO_PHON': None}
Experiments are proposed to provide a critical assessment of the                use of (14C)-2-deoxglucose and specifically labeled (14C)-glucose               in the measurement of cerebral functional activity.  The uptake                 and retention of radioactivity in discrete brain regions from                   (14C)-2-deoxyglucose, (1-14C)-glucose and (6-14C)-glucose will be               evaluated with high resolution autoradiography in which 4 um                    frozen brain sections are thaw-mounted onto nuclear emulsion                    coated slides.  These studies will delineate the similarities and               differences in the relative uptake and retention of radioactivity               from the various compounds and in glucose utilization rates                     determined with the appropriate kinetic models.  The hypothesis                 will be tested that the previously demonstrated low retention of                radioactivity from (14C)-2-DG in some brain regions relative to                 that from (1-14C)-glucose is due to regionally high glucose-6-                  phosphatase activity, low phosphorylation of 2-DG, or low                       transport of (14C)-2-deoxyglucose-6- phosphate accumulation will                be measured in microdissected brain regions by liquid                           scintillation counting in rats killed by microwave radiation, 1                 to 45 minutes after i.v. injection of (14C)-deoxyglucose.  If                   some brain regions contain high glucose-6-phosphate activity, an                initial accumulation, followed by a decline in 2-deoxyglucose-6-                phosphate, is predicted.  If a brain region exhibits low                        phosphorylation of (14C)-2-DG, little accumulation of (14C)-2-DG                is not transported as effectively into the cells of certain                     regions as is glucose, low levels of accumulation of                            radioactivity from (14C)-2-DG will occur at all times.  The                     ability of (14C)-2-DG and specifically labelled (14C)-glucose to                register alterations in functional activity will be determined by               sensory stimulation and by stimulating a well defined afferent                  pathway to the hippocampal formation.  Concurrent                               electrophysiological recording will be performed to determine the               relationship of metabolic and electrical activities.
{'FirstName': 'Gary', 'LastName': 'Duncan', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Gary E Duncan', 'EmailAddress': None, 'NSF_ID': '000261324', 'StartDate': '05/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of North Carolina at Chapel Hill', 'CityName': 'CHAPEL HILL', 'ZipCode': '275995023', 'PhoneNumber': '9199663411', 'StreetAddress': '104 AIRPORT DR STE 2200', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'North Carolina', 'StateCode': 'NC', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'NC04', 'ORG_UEI_NUM': 'D3LHU66KBLD5', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL', 'ORG_PRNT_UEI_NUM': 'N8DMK1K4C2K5'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1192', 'Text': 'NEURONAL AND GLIAL MECHANISMS'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8721777.xml'}