Genetics and Biochemical Studies of the ATP Synthase
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The goal of this project to gain an understanding of the                        structure and function of mitochondrial ATP synthase.                           Specifically, molecular genetic approaches will be applied to                   an investigation of two key peptides of the oligomeric enzyme                   from yeast: 1) the beta subunit of the F1-ATPase, and 2) the                    oligomycin sensitive conferring peptide (OSCP) that links F1                    with F0 domain.  The beta subunit is thought to be at least a                   part of the catalytic site for ATP synthesis.  The role of                      specific amino acids in catalysis or binding will be                            evaluated by site directed mutagenesis using oligonucleotides                   and by random mutagenesis using chemical methodology.  The                      mutations will be studied by introducing the mutated genes                      into a beta subunit deficient yeast strain determining its                      effect on function.  For the OSCP unit the gene will be                         cloned, sequenced and studied in a similar manner as is                         planned for the beta subunit.  The results of the research                      should enhance our understanding of how ATP, the key compound                   involved in transduction of energy in biological systems, is                    synthesized.
{'FirstName': 'David', 'LastName': 'Mueller', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David M Mueller', 'EmailAddress': None, 'PI_PHON': None, 'NSF_ID': '000307538', 'StartDate': '02/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'ROSALIND FRANKLIN UNIVERSITY OF MEDICINE AND SCIENCE', 'CityName': 'NORTH CHICAGO', 'ZipCode': '600643037', 'PhoneNumber': '8475788524', 'StreetAddress': '3333 GREEN BAY RD', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Illinois', 'StateCode': 'IL', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'IL10', 'ORG_UEI_NUM': 'V913TCT2SFZ9', 'ORG_LGL_BUS_NAME': 'ROSALIND FRANKLIN UNIVERSITY OF MEDICINE & SCIENCE', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1144', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8703618.xml'}