Molecular Genetics of Ribosomal RNA
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
We propose to develop genetic approaches to the study of                        ribosomal RNA in E. coli.  The main feature of the method is to                 use antibiotic-resistance mutations in 16S and 23S rRNA as                      genetic markers.  Another important aspect is the use of rapid                  methodologies that will permit isolation and characterization                   of large numbers (hundreds) of mutants.  Mutations that confer                  a variety of phenotypes, including temperature-sensitive,                       "downs," nulls, Ram, suppressors, etc. will be isolated and                     localized by marker rescue and DNA sequencing.  Second site                     revertants will also be sought, in an effort to identify long-                  range structural and functional interactions involving                          ribosomal RNA.
{'FirstName': 'Harry', 'LastName': 'Noller', 'PI_MID_INIT': 'F', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Harry F Noller', 'EmailAddress': 'harry@nuvolari.ucsc.edu', 'NSF_ID': '000440375', 'StartDate': '09/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of California-Santa Cruz', 'CityName': 'SANTA CRUZ', 'ZipCode': '950641077', 'PhoneNumber': '8314595278', 'StreetAddress': '1156 HIGH ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '19', 'CONGRESS_DISTRICT_ORG': 'CA19', 'ORG_UEI_NUM': 'VXUFPE4MCZH5', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF CALIFORNIA SANTA CRUZ', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '111200', 'Text': 'Genetic Mechanisms'}
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