Early Regulatory Mechanisms of Bacteriophage T4
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
The proposed research investigates basic mechanisms in virus-                   host interactions, specifically the shift from host to viral                    transcription, using the E. coli-bacteriophage T4 system.                       Emphasis is placed upon the T4 gene product alc/unf and its                     interaction with other factors in shutting off transcription                    from cytosine-containing DNA and unfolding the host nucleoid.                                                                                                   Our recent work has established that gpalc generally inhibits                   transcription from dC-DNA both in vivo and in vitro, probably                   by promoting pausing and termination of elongating RNA                          transcripts.  Proposed research includes: 1) Completion of                      cloning of the cDNA for alc into an expression vector and                       purification of the alc protein; 2) use of the purified protein                 to further study the inhibition by gpalc of RNA chain                           elongation; 3) investigation of the interaction of gpalc with                   other factors involved in transcriptional pausing and                           termination; 4) determination of whether gpalc acts through                     binding to DNA, RNA polymerase, or a complex; 5) analysis of                    sites on DNA where gpalc binding occurs; 6) studies of the                      relationship between the observed transcriptional inhibition                    and the other known effect of gpalc/unf - the unfolding of the                  host nucleoid; and 7) studies to determine what other T4 genes                  are also involved in shutoff of host transcription.
[{'FirstName': 'Burton', 'LastName': 'Guttman', 'PI_MID_INIT': 'S', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Burton S Guttman', 'EmailAddress': 'guttmanb@evergreen.edu', 'NSF_ID': '000381099', 'StartDate': '08/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Elizabeth', 'LastName': 'Kutter', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Elizabeth M Kutter', 'EmailAddress': 'kutterb@evergreen.edu', 'NSF_ID': '000429220', 'StartDate': '08/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Rolf', 'LastName': 'Drivdahl', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Rolf H Drivdahl', 'EmailAddress': None, 'NSF_ID': '000114723', 'StartDate': '08/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Evergreen State College', 'CityName': 'OLYMPIA', 'ZipCode': '985050001', 'PhoneNumber': '3608676640', 'StreetAddress': '2700 EVERGREEN PKWY NW', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Washington', 'StateCode': 'WA', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'WA10', 'ORG_UEI_NUM': 'CY9BGL4NJUP4', 'ORG_LGL_BUS_NAME': 'THE EVERGREEN STATE COLLEGE', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8704366.xml'}