Response of Anchorage-Dependent Animal Cells to Fluid Shear Stress
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Maria Burka', 'PO_EMAI': None, 'PO_PHON': None}
Recently, there has been increased interest in growing                          anchorage-dependent animal cells for the production of medically                important biochemicals as well as for the possible use of these cells           in artificial organs.  The interaction of these cells with the in-vitro         environment is poorly understood.  The objective of this study is to            examine the response of anchorage-dependent animal cells to fluid shear         stresses.  A parallel plate flow channel will be constructed and tested         to expose the cells to a well-defined laminar flow field.  Cell                 detachment from the surface as well as nonlethal changes in cell                behavior and metabolism (such as increased permeability and                     morphological changes) and protein secretion will be examined as a              function of the magnitude and duration of the applied shear stress.             The effect of the surface on cell detachment and other changes in cell          behavior will be examined by studying the response to shear stress of           cells grown on a variety of natural and synthetic surfaces.
{'FirstName': 'George', 'LastName': 'Truskey', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'George A Truskey', 'EmailAddress': 'gtruskey@acpub.duke.edu', 'NSF_ID': '000221449', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Tufts University', 'CityName': 'SOMERVILLE', 'ZipCode': '021442401', 'PhoneNumber': '6176273696', 'StreetAddress': '169 HOLLAND ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '07', 'CONGRESS_DISTRICT_ORG': 'MA07', 'ORG_UEI_NUM': 'WL9FLBRVPJJ7', 'ORG_LGL_BUS_NAME': 'TRUSTEES OF TUFTS COLLEGE', 'ORG_PRNT_UEI_NUM': 'S6ZWCYHRQQB3'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1402', 'Text': 'BIOCHEMICAL & BIOMASS ENG'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8612586.xml'}