Mechanism and Function of Serine Hydroxymethyltransferase
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Eve Ida Barak', 'PO_EMAI': None, 'PO_PHON': None}
The major source of one-carbon groups in the cell is the                        conversion of serine to glycine and methylene-H4folate.  In                     addition to H4folate this reaction also requires pyridoxal 5'                   phosphate (PLP).  The reaction is catalyzed by the enzyme                       serine hydroxymethyltransferase (SHMT).  This proposal                          focuses on elucidating its structure, regulation, and                           mechanism.  Under the previous grant the enzyme was                             purified to homogeneity from several sources and several more                   purifications are underway.  Site-specific mutagenesis will                     be used to determine the function of several amino acid                         residues at the active site in the E. coli enzyme.  Next the                    structural genes from rabbitt liver which code for cytosolic                    and mitochondrial SHMT will be cloned.  The genes will be                       isolated sequenced and expressed in a procaryotic plasmid                       system. Additionally, the function of mitochondrial SHMT will                   be determined.
{'FirstName': 'LaVerne', 'LastName': 'Schirch', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'LaVerne Schirch', 'EmailAddress': None, 'NSF_ID': '000303408', 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Virginia Commonwealth University', 'CityName': 'RICHMOND', 'ZipCode': '232849005', 'PhoneNumber': '8048286772', 'StreetAddress': '910 WEST FRANKLIN ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Virginia', 'StateCode': 'VA', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'VA04', 'ORG_UEI_NUM': 'MLQFL4JSSAA9', 'ORG_LGL_BUS_NAME': 'VIRGINIA COMMONWEALTH UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1144', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8704031.xml'}