Research Initiation: Protein Elicitation of Secondary       Metabolites in Cultured Plant Cells
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
RESEARCH INITIATION AWARD:                                                                                                                            PROTEIN ELICITATION OF SECONDARY METABOLITES IN                                              CULTURED PLANT CELLS                                                                                                                                                  ABSTRACT                                                                                                                            This proposal attempts to:                                                                                                                                           - determine the mechanism of elicitor activity by placing a                       recognized elicitor protein inside a cell and monitoring                        the cell's response, thereby bypassing the hypothesized                         polysaccharide receptor sites on the outer wall, and                                                                                                          - evaluate the observation that tyrosine decarboxylase (TDC)                      levels correlate with synthesis of a secondary metabolite,                      berberine.                                                                                                                                               The resulting data are to be used to develop kinetic models for                 growth rate, product synthesis and secretion rates which reflect                the dependence on elicitor concentration.  Until more detail is                 known regarding the metabolic control involved in secondary                     metabolite production, effective use of plant cell culture will be              extremely difficult to achieve.
{'FirstName': 'Steven', 'LastName': 'Peretti', 'PI_MID_INIT': 'W', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Steven W Peretti', 'EmailAddress': 'peretti@eos.ncsu.edu', 'NSF_ID': '000190036', 'StartDate': '06/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'North Carolina State University', 'CityName': 'RALEIGH', 'ZipCode': '276950001', 'PhoneNumber': '9195152444', 'StreetAddress': '2601 WOLF VILLAGE WAY', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'North Carolina', 'StateCode': 'NC', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'NC02', 'ORG_UEI_NUM': 'U3NVH931QJJ3', 'ORG_LGL_BUS_NAME': 'NORTH CAROLINA STATE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8809319.xml'}