Genome Structure and Replication of a Plant Rhabdovirus
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
This study has three primary objectives:  l) to continue                        studies on the structure and organization of the RNA of sonchus                 yellow net virus (SYNV), a plant rhabdovirus; 2) to infect                      tobacco protoplasts with SYNV so that kinetics and subcellular                  location of the viral specific RNAs and proteins can be                         investigated during synchronous replication; and 3) to isolate                  a transcriptase from infected protoplasts.  Completion of                       objective l will provide a precise description of the genome of                 a plant rhabdovirus that can be used to compare its structure,                  function, and evolution with related animal rhabdoviruses.                      Infection of protoplasts with SYNV (Objective 2) will allow                     analysis of the kinetics of synthesis of SYNV RNAs and                          proteins at defined stages of replication and will permit                       investigation of their association with host polypeptides and                   cytopathic structures that appear during infection.  Isolation                  of a transcriptase complex (Objective 3) from infected                          protoplasts will facilitate in vitro studies of the functions                   of viral specific components and the requirement for host                       factors during transcription of messenger RNAs and replication                  of SYNV RNA.
{'FirstName': 'Andrew', 'LastName': 'Jackson', 'PI_MID_INIT': 'O', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Andrew O Jackson', 'EmailAddress': 'andyoj@uclink4.berkeley.edu', 'NSF_ID': '000351311', 'StartDate': '03/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of California-Berkeley', 'CityName': 'BERKELEY', 'ZipCode': '947101749', 'PhoneNumber': '5106433891', 'StreetAddress': '1608 4TH ST STE 201', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '12', 'CONGRESS_DISTRICT_ORG': 'CA12', 'ORG_UEI_NUM': 'GS3YEVSS12N6', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF CALIFORNIA, THE', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '1116', 'Text': 'EUKARYOTIC GENETICS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8716467.xml'}