Enzymes Catalyzing the Biosynthesis of Alkaloids            (Chemistry)
{'Code': '03090003', 'Directorate': {'Abbreviation': 'MPS', 'LongName': 'Direct For Mathematical & Physical Scien'}, 'Division': {'Abbreviation': 'CHE', 'LongName': 'Division Of Chemistry'}}
{'SignBlockName': 'George M. Rubottom', 'PO_EMAI': None, 'PO_PHON': None}
This research project in the Organic and Macromolecular Chemistry               Program is designed to provide deeper understanding of how                      certain key organic compounds are synthesized in plants.                                                                                                        Cell-free systems containing specific enzymes will be obtained                  from various parts of alkaloid-producing plants.  The ability of                these cell-free extracts to catalyze the transformation of known                or suspected intermediates in the biosynthesis of the ultimate                  alkaloids will be examined.  In general, the enzymes will be                    assayed by the use of specifically labeled (carbon, tritium, and                deuterium) substrates, the use of which will facilitate                         identification of products and transient intermediates.  Some                   transformations which will be studied (with the potential source                of the enzymes indicated in parenthesis) are the following: (1)                 the demethylation of nicotine (Nicotinia glutinosa leaves); (2)                 cocaine biosynthesis (Erythroxylon coca leaves); (3) phenylalanine              conversion to (s)-tropic acid (Danutura innoxia roots); (4)                     tropinone conversion to tropine (Datura innoxia roots); (5)                     octanoic acid conversion to 5-oxo-octanoic acid (Conium maculatum               leaves); and (6) o-succinyl benzoic acid conversion to shihunine                (Dendrobium pierardii stems and leaves).
{'FirstName': 'Edward', 'LastName': 'Leete', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Edward Leete', 'EmailAddress': None, 'NSF_ID': '000382834', 'StartDate': '09/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Minnesota-Twin Cities', 'CityName': 'MINNEAPOLIS', 'ZipCode': '554552009', 'PhoneNumber': '6126245599', 'StreetAddress': '200 OAK ST SE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MN05', 'ORG_UEI_NUM': 'KABJZBBJ4B54', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF MINNESOTA', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1950', 'Text': 'METHODOLOGY'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8620345.xml'}