Equipment Grant:  Macromolecular Transport in Normal and    Tumor Capillary Beds (Effects in Charge and Configuration)
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Robert M. Wellek', 'PO_EMAI': None, 'PO_PHON': None}
The overall aim of this study is to quantify the effects of   molecular         configuration and charge on the transport of macromolecules in normal           and tumor capillary-beds.  These molecules include well characterized           dextrans, immunoglobulins, and monoclonal antibodies, all tagged with           fluorescein isothiocyanate (FITC).  Experimental methods involve                growing normal (mature granulation) and tumor (VX2 Carcinoma) tissues           in a transparent rabbit ear chamber prepared routinely in the CMU               laboratory.  Once the tissue preparation is mature, FITC tagged                 molecules are injected into the animal's circulation.  Resulting                extravasation of molecules is video recorded for up to three hours              using an intravital fluorescene microscopy technique developed in the           laboratory.  Since the intensity of the photo-signal is very low (i.e.          10-6 ft- candle), a highly sensitive video camera will be purchased.            Increased sensitivity and camera resolution enable transport over a             small segment of a blood vessel to be monitored..
{'FirstName': 'Rakesh', 'LastName': 'Jain', 'PI_MID_INIT': 'K', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Rakesh K Jain', 'EmailAddress': None, 'NSF_ID': '000237802', 'StartDate': '12/15/1984', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Carnegie-Mellon University', 'CityName': 'PITTSBURGH', 'ZipCode': '152133815', 'PhoneNumber': '4122688746', 'StreetAddress': '5000 FORBES AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '18', 'CONGRESS_DISTRICT_ORG': 'PA18', 'ORG_UEI_NUM': 'U3NKNFLNQ613', 'ORG_LGL_BUS_NAME': 'CARNEGIE MELLON UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'U3NKNFLNQ613'}
{'Name': 'Carnegie-Mellon Institute of Technology', 'CityName': 'PITTSBURGH', 'StateCode': 'PA', 'ZipCode': '152133815', 'StreetAddress': '5000 FORBES AVE', 'CountryCode': 'US', 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'CountryFlag': '1', 'CONGRESSDISTRICT': '18', 'CONGRESS_DISTRICT_PERF': 'PA18'}
{'Code': '1414', 'Text': 'INTERFAC PROCESSES & THERMODYN'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1984&All=true', 'xml': '8413191.xml'}