RUI Regulation of the Bacillus subtillis Inducible DNA      Repair System
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
The overall objective of the proposed research is to identify                   and isolate the regulatory components of the Bacillus subtilis                  SOS DNA repair system and start to characterize the molecular                   details of its regulation.  Evidence available to date suggests                 that the well-characterized Escherichia coli SOS system may be                  highly conserved in B. subtilis. In E. coli, induction of the                   SOS DNA repair genes is due to the RecA protein-mediated                        cleavage of the SOS gene repressor, LexA protein.  It appears                   that an analogous mechanism, involving the B. subtilis RecA                     protein analogue, occurs in B. subtilis.  The specific aims of                  this project are to (1) characterize the regulatory DNA                         sequences of four distinct B. subtilis DNA damage-inducible                     genes; (2) purify the protein (presumably a LexA analogue) that                 binds to these sequences and clone the corresponding gene; and                  (3) characterize the interactions of this protein with the                      regulatory DNA sequences and with the B. subtilis RecA protein                  analogue.  The methods will include using the regulatory DNA                    sequences to (1) identify the protein and track its                             purification in DNase footprinting assays; (2) construct an                     affinity column to facilitate the protein purification; and (3)                 probe a lambda gt11 library for the gene that codes for the                     protein.                                                                                                                                                        At present, the regulation of inducible DNA repair is only                      understood for E. coli.  This research will be a decisive step                  toward understanding the regulation of the SOS DNA repair                       system in B. subtilis, an organism that is not closely related                  to E. coli.
{'FirstName': 'Charles', 'LastName': 'Lovett', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': 'Jr', 'PI_FULL_NAME': 'Charles M Lovett', 'EmailAddress': 'clovett@williams.edu', 'NSF_ID': '000085365', 'StartDate': '01/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Williams College', 'CityName': 'WILLIAMSTOWN', 'ZipCode': '012672600', 'PhoneNumber': '4135974352', 'StreetAddress': '880 MAIN ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'MA01', 'ORG_UEI_NUM': 'JVZEJJ6N5EM8', 'ORG_LGL_BUS_NAME': 'PRESIDENT & TRUSTEES OF WILLIAMS COLLEGE', 'ORG_PRNT_UEI_NUM': 'JVZEJJ6N5EM8'}
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[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8818644.xml'}