Structure of Microbial Communities Through 16S rRNA         Analysis:  cDNA and Hybridization Probe Methods
{'Code': '08010208', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DEB', 'LongName': 'Division Of Environmental Biology'}}
{'SignBlockName': 'Althea Ball', 'PO_EMAI': 'aball@nsf.gov', 'PO_PHON': '7032920000'}
Microbial ecologists are unsure of the composition and                     structure of any microbial community, because traditional methods               cannot ensure the recognition of all community members.  This                   provides a poor basis for understanding how toxicants and                       genetically engineered organisms affect natural microbial                       communities.  New solutions to this problem come from molecular                 phylogeny and improvements in recombinant-DNA technology.  16S                  rRNA is a unique cellular component useful for describing                       microbial communities.  Prior work on this approach leads to the                proposed development of a method for retrieving 16S rRNA sequence               information from natural communities as copy DNA, valuable in                   that it permits quantitative as well as qualitative analysis of                 community members.  The limitations and scope of the method will                be analyzed on a hot spring cyanobacterial mat community as a                   model.
{'FirstName': 'David', 'LastName': 'Ward', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David M Ward', 'EmailAddress': 'umbdw@montana.edu', 'NSF_ID': '000283247', 'StartDate': '01/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
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[{'Code': '1128', 'Text': 'Population & Community Ecology'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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