Characterization, Manipulation, and Modeling of Membrane-   Bound Yeast Alcohol Dehydrogenase Used for a High-Loading   Analytical Application
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Maria Burka', 'PO_EMAI': None, 'PO_PHON': None}
The objective of this research is to characterize, manipulate and model         the immobilized enzymes used for a high loading analytical sensing              application.  The main goal is to experimentally explore the                    relationship between phenomological performance (activity, lifetime)            and enzyme loading, distribution and lability as observed with direct           techniques in a slab geometry, membrane system.  Experiments will be            performed with membrane-bound yeast alcohol dehydrogenase (YADH) that           consist of: (1) characterizing the distribution of labelled YADH within         the membrane, (2) examining the intrinsic lability of bound YADH with           differential scanning calorimetry, and (3) correlating these direct             observations with the activity and lifetime of membrane-bound YADH              where substrate flux is either convective or diffusive.
{'FirstName': 'Michael', 'LastName': 'Domach', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Michael M Domach', 'EmailAddress': 'md0q@andrew.cmu.edu', 'NSF_ID': '000100579', 'StartDate': '03/01/1986', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Carnegie-Mellon University', 'CityName': 'PITTSBURGH', 'ZipCode': '152133815', 'PhoneNumber': '4122688746', 'StreetAddress': '5000 FORBES AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '12', 'CONGRESS_DISTRICT_ORG': 'PA12', 'ORG_UEI_NUM': 'U3NKNFLNQ613', 'ORG_LGL_BUS_NAME': 'CARNEGIE MELLON UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'U3NKNFLNQ613'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1402', 'Text': 'BIOCHEMICAL & BIOMASS ENG'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1985&All=true', 'xml': '8513443.xml'}