Molecular Mapping of the Chlamydomonas Genome
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
The unicellular green alga Chlamydomonas reinhardtii has                        several unique characteristics that make it the model system of                 choice for molecular genetic studies of genes involved in                       eukaryotic flagella, chloroplasts, cell-cell interactions, and                  a number of biochemical pathways.  We will develop a molecular                  map of the Chlamydomonas genome to complement the extensive                     genetic map that currently exists.  Restriction fragment length                 polymorphism that distinguish the DNA from two interfertile                     strains will be used to follow the segregation of molecular                     markers in genetic crosses.  Molecular markers will be mapped                   with respect to phenotypic markers on each of the nineteen                      linkage groups.  Sets of linked molecular markers will be ordered               on each linkage group.  The ends of linkage groups will be mapped               using molecular markers associated with telomere.  Precise                      correlation between the molecular map and the genetic map will                  be sought by carrying out a "chromosome walk" in one region of                  the genome.  This project will greatly facilitate molecular and                 genetic analysis of Chlamydomonas nuclear genes currently                       underway in many laboratories including our own.                                                                                                                The single-celled green alga Chlamydomonas has become an                        important model organism for the study of a number of problems                  of fundamental biological interest.  This is a proposal to                      develop a detailed and comprehensive map of the DNA and genes                   of this organism.  Such a map will be a valuable resource to                    workers in the field, and will greatly facilitate their work.
[{'FirstName': 'Paul', 'LastName': 'Lefebvre', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Paul A Lefebvre', 'EmailAddress': 'pete@umn.edu', 'NSF_ID': '000245634', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Carolyn', 'LastName': 'Silflow', 'PI_MID_INIT': 'D', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Carolyn D Silflow', 'EmailAddress': 'silfl001@umn.edu', 'NSF_ID': '000412779', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'University of Minnesota-Twin Cities', 'CityName': 'MINNEAPOLIS', 'ZipCode': '554552009', 'PhoneNumber': '6126245599', 'StreetAddress': '200 OAK ST SE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MN05', 'ORG_UEI_NUM': 'KABJZBBJ4B54', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF MINNESOTA', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '111600', 'Text': 'EUKARYOTIC GENETICS'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
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