Membrane-Based Process for the Removal of Toxic Ammonium IonProduced During Mammalian-Cell Culture
{'Code': '15030000', 'Directorate': {'Abbreviation': 'TIP', 'LongName': 'Dir for Tech, Innovation, & Partnerships'}, 'Division': {'Abbreviation': 'TI', 'LongName': 'Translational Impacts'}}
{'SignBlockName': 'Ritchie B. Coryell', 'PO_EMAI': None, 'PO_PHON': None}
Mammalian cells grown in culture excrete ammonium ion that                 accumulates in quantities that may limit cell growth and reduce                 product synthesis.  The usual tactic for limiting the                           accumulation of ammonia is to replace the "spent" medium with                   fresh medium.  Since medium replacement increases the use of                    expensive medium components, ammonium accumulation lowers cell                  and product yield on medium and increases production costs.  An                 attractive strategy for nullifying the problems associated with                 ammonium ion excretion is to provide a continuous process for                   removing ammonium ion and maintaining it at a subinhibitory,                    steady-state concentration.  In this proposal, Dr. Van Eikeren                  describes a membrane-based process for the selective and                        continuous removal of ammonium ions produced during mammalian                   cell culture. Preliminary technical and economic analyses suggest               that the process proposed will be effective.                                        A membrane-based process  for ammonia removal, coupled to a                 fermenter would be valuable for growing mammalian cells for the                 purpose of manufacturing protein products.
{'FirstName': 'Paul', 'LastName': 'van Eikeren', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Paul van Eikeren', 'EmailAddress': 'paul.van.eikeren@intellichem.com', 'PI_PHON': '5413827043', 'NSF_ID': '000302473', 'StartDate': '02/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Bend Research Inc', 'CityName': 'Bend', 'ZipCode': '977018599', 'PhoneNumber': '5413824100', 'StreetAddress': '64550 Research Road', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Oregon', 'StateCode': 'OR', 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_ORG': None, 'ORG_UEI_NUM': None, 'ORG_LGL_BUS_NAME': None, 'ORG_PRNT_UEI_NUM': None}
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{'Code': '5371', 'Text': 'SBIR Phase I'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8760538.xml'}