Factors Modulating Responses to Nerve Growth Factor
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Steven C. McLoon', 'PO_EMAI': None, 'PO_PHON': None}
During maturation and aging, the nervous system exhibits a                      progressive decline in plasticity and capacity for repair.  This                decline is similar to the decline in responsiveness with the age                of target cells to nerve growth factor (NGF).  In these cells,                  NGF is specifically bound by cell surface receptors,                            internalized, transported to the cell body and degraded in                      lysosomal organelles.  Dr. Claude will begin investigation of                   these steps in order to gain a better understanding of the                      mechanism of action of NGF, and of the ways in which target cell                response is modified by age and by other factors, including                     glucocorticoid hormones.                                                                                                                                        The experiments will make use of dissociated cell cultures of                   adrenal chromaffin cells from rats of different ages.  These                    cells respond to NGF with neuritic outgrowth.  Dr. Claude will                  monitor the number and distribution of NGF receptors on the                     surface of the target cells as a function of (1) the age of the                 cell, and (2) the presence of glucocorticoid hormones.  The                     techniques to be used include neural tissue culture, electron                   microscopy (EM), and cell surface labelling with visible markers                (colloidal gold).  The information to be gained from these                      experiments will help us to understand the mechanism of action of               NGF, and provide insights into some of the changes that neurons                 undergo during development, maturation and aging.
{'FirstName': 'Philippa', 'LastName': 'Claude', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Philippa Claude', 'EmailAddress': None, 'NSF_ID': '000108190', 'StartDate': '08/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Wisconsin-Madison', 'CityName': 'MADISON', 'ZipCode': '537151218', 'PhoneNumber': '6082623822', 'StreetAddress': '21 North Park Street', 'StreetAddress2': 'Suite 6301', 'CountryName': 'United States', 'StateName': 'Wisconsin', 'StateCode': 'WI', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'WI02', 'ORG_DUNS_NUM': '161202122', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF WISCONSIN SYSTEM', 'ORG_PRNT_DUNS_NUM': '041188822'}
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{'Code': '1190', 'Text': 'DEVELOPMENTAL NEUROSCIENCE'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8616958.xml'}