RUI:  Calcium Regulation of Brain Gangliosides
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
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The proposed study will test the hypothesis that chronic changes                in extracellular calcium levels will alter the relative                         concentration or turnover of selected ganglioside species.  In                  the first in vitro phase, extracellular calcium will be                         manipulated in incubating tissue slices in a physiological medium               containing variable calcium (and control) ion concentrations                    after preloading the tissue with radiolabeled ganglioside                       precursors.  In a subsequent in vivo phase, animals will be                     injected to maximally label gangliosides, then chronic serum                    calcium levels will be manipulated by a combination of endocrine                surgery and diet control.  Gangliosides will be extracted from                  membranous fractions of cerebrocortical, hippocampal, and                       thalamic tissue, then purified and separated by two-dimensional                 thin-layer chromatography.  The content of individual                           gangliosides will be determined by scanning densitometry coupled                with computer-assisted image analysis.  Turnover of individual                  ganglioside species will be determined by scanning densitometry                 of autoradiograms.  The ultimate aim of this research is to shed                light on the role of gangliosides and their interactions with                   calcium.  This question is of fundamental importance in basic                   neuroscience, but heightened public interest in dietary calcium                 gives the topic timely relevance as well.
{'FirstName': 'Louis', 'LastName': 'Irwin', 'PI_MID_INIT': 'N', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Louis N Irwin', 'EmailAddress': 'lirwin@utep.edu', 'NSF_ID': '000371838', 'StartDate': '07/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Simmons University', 'CityName': 'BOSTON', 'ZipCode': '021155820', 'PhoneNumber': '6175212824', 'StreetAddress': '300 FENWAY', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '07', 'CONGRESS_DISTRICT_ORG': 'MA07', 'ORG_UEI_NUM': 'SAKCL8BJ6DL9', 'ORG_LGL_BUS_NAME': 'SIMMONS UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'SAKCL8BJ6DL9'}
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[{'Code': '1192', 'Text': 'NEURONAL AND GLIAL MECHANISMS'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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