Recombinant-DNA Alterations, and Genetic and Enzymatic      Characterization of Anaerobic Fermentation Process Importantto Biocatalysis by Butric-Acid Bacteria (Clostridia)
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
Efforts are underway to investigate and develop methodologies for the           transformation of butyric-acid clostridia (Clostridium acetobutylicum           and related strains) and to investigate the fundamental and applied             aspects of their molecular biology and genetics for biocatialysis               applications.  Little is known about the genetics or regulation of              enzymatic activities involved in the formation of primary metabolism            products (organic acids and solvents) of strict anaerober such as the           butyric-acid clostridia.  Mutants deficient in enzymes of solvent               forming pathways will be isolated and characterized.  These mutant              strains will allow the cloning and characterization of genes which              complement the deficiencies.  This approach will not only allow                 isolation of the structural genes of the enzymes directly involved, but         also enable regulatory elements which may be necessary for expression           of these genes to be identified and analyzed.
[{'FirstName': 'Frederick', 'LastName': 'Rudolph', 'PI_MID_INIT': 'B', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Frederick B Rudolph', 'EmailAddress': 'fbr@bioc.rice.edu', 'NSF_ID': '000398628', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'George', 'LastName': 'Bennett', 'PI_MID_INIT': 'N', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'George N Bennett', 'EmailAddress': 'gbennett@rice.edu', 'NSF_ID': '000404375', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Eleftherios', 'LastName': 'Papoutsakis', 'PI_MID_INIT': 'T', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Eleftherios T Papoutsakis', 'EmailAddress': 'papoutsakis@dbi.udel.edu', 'NSF_ID': '000293098', 'StartDate': '09/01/1986', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'William Marsh Rice University', 'CityName': 'Houston', 'ZipCode': '770051827', 'PhoneNumber': '7133484820', 'StreetAddress': '6100 MAIN ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '09', 'CONGRESS_DISTRICT_ORG': 'TX09', 'ORG_UEI_NUM': 'K51LECU1G8N3', 'ORG_LGL_BUS_NAME': 'WILLIAM MARSH RICE UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '149100', 'Text': 'Cellular & Biochem Engineering'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8613077.xml'}