Adrenal Medullary Enkephalin-Containing Peptides
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Stacy Springer', 'PO_EMAI': None, 'PO_PHON': None}
Although the amino acid sequence and the identities of many                     processing products of proenkephalin are known, little                          information is available on the rates of proenkephalin synthesis                and degradation or on the details of proenkephalin processing and               storage.  The first aim of this project is to determine the rates               of proenkephalin synthesis and degradation.  Bovine adrenal                     medullary chromaffin cells will be incubated with radiolabeled                  amino acids, and their incorporation into enkephalin sequences in               the various proenkephalin fragments will be determined following                fractionation by high performance liquid chromatography.  The                   second aim is to determine the pathway of proenkephalin                         processing.  Pulse-chase radiolabeling of enkephalin sequences                  within chromaffin cell proenkephalin will be used to study this                 pathway.  The third aim is to determine the second messenger(s)                 responsible for regulation of proenkephalin synthesis and                       processing.  The roles of calcium, cyclic AMP, and other agents,                including glucocorticiods, will be evaluated.  The forth aim is                 to determine the relationship between proenkephalin and                         chromaffin vesicle synthesis.  Vesicles from radiolabeled                       chromaffin cells will be subjected to density gradient                          centrifugation; fractions will be analyzed for newly synthesized                enkephalin sequences and other vesicle components.  These studies               will elucidate the basic mechanisms of neurotransmitter-hormone                 synthesis and storage.
{'FirstName': 'Steven', 'LastName': 'Wilson', 'PI_MID_INIT': 'P', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Steven P Wilson', 'EmailAddress': 'sw@sc.edu', 'NSF_ID': '000207964', 'StartDate': '04/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of South Carolina at Columbia', 'CityName': 'COLUMBIA', 'ZipCode': '292083403', 'PhoneNumber': '8037777093', 'StreetAddress': '1600 HAMPTON ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'South Carolina', 'StateCode': 'SC', 'CONGRESSDISTRICT': '06', 'CONGRESS_DISTRICT_ORG': 'SC06', 'ORG_UEI_NUM': 'J22LNTMEDP73', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF SOUTH CAROLINA', 'ORG_PRNT_UEI_NUM': 'J22LNTMEDP73'}
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[{'Code': '1188', 'Text': 'SYNAPTIC MECHANISMS'}, {'Code': '1192', 'Text': 'NEURONAL AND GLIAL MECHANISMS'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8719149.xml'}