The Mechanism and Regulation of Ovulation in Fish
{'Code': '08090300', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Vincent P. Gutschick', 'PO_EMAI': None, 'PO_PHON': None}
Past research implicates eicosanoids, the phosphatidylinositol/protein          kinase C system and proteolytic enzymes in fish ovulation. The proposal         research will increase our understanding of the role of these three             mediators in fish ovulation.  Specifically, the production of                   lipoxygense metabolites by brook trout and goldfish ovaries will be             investigated using radio labeled precursor incorporation, followed by           metabolite separation on high performance liquid chromatography.  The           production of primary prostaglandins during steroid-induced ovulation           in yellow perch will be investigated using radioimmunoassay.  An                incorporation system, involving the labeling of follicular                      phosphatidylinositol by radioactive myoinositol, will be investigated           so that future research can use this system to study the cycling of             follicular inositol phosphates following agonist stimulation.  Total            follicular proteolytic activity thoughout maturation and ovulation will         be followed in brook trout and goldfish using substrate polyacrylamide          gel electrophoresis.  Collagenolytic activity will be followed through          ovulation in brook trout follicles using a specific substrate assay to          determine the role of this proteases in the release of the eggs from            the follicles.  The goal is a better understanding of the endocrine             processes regulating ovulation in fish.
{'FirstName': 'Frederick', 'LastName': 'Goetz', 'PI_MID_INIT': 'W', 'PI_SUFX_NAME': 'Jr.', 'PI_FULL_NAME': 'Frederick W Goetz', 'EmailAddress': 'rick@uwm.edu', 'NSF_ID': '000193499', 'StartDate': '04/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Notre Dame', 'CityName': 'NOTRE DAME', 'ZipCode': '465565708', 'PhoneNumber': '5746317432', 'StreetAddress': '940 GRACE HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Indiana', 'StateCode': 'IN', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'IN02', 'ORG_UEI_NUM': 'FPU6XGFXMBE9', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF NOTRE DAME DU LAC', 'ORG_PRNT_UEI_NUM': 'FPU6XGFXMBE9'}
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{'Code': '114100', 'Text': 'PHYSIOLOG & STRUCTURAL SYS'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8718178.xml'}