Regulation of Iron Transport and Virulence in Shigella
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
Iron is an essential, but elusive element for most cells, and                   high affinity transport systems are usually required for its                    acquisition.  The long range goals of this project are to                       characterize the regulation of the iron acquisition systems and                 other iron regulated genes of the enteric bacterial genus,                      Shigella.  These studies will include (1) characterizing the                    mechanisms of regulation of enterobactin expression in Shigella                 flexneri, (2) measuring the expression of iron-regulated genes in               an in vivo system, and (3) characterizing the positive and                      negative regulatory elements which affect expression of the gene                for Congo red/heme binding (crb).  Experimental approaches will                 utilize genetic and biochemical techniques.  In vivo expression                 of the genes will be investigated using fusions to reporter                     genes, such as lux, whose expression can be readily monitored,                  and an in vivo labeling system has been developed to compare                    proteins synthesized by bacteria inside host cells to those                     observed in vitro.  Characterization of mutants with altered gene               regulation is also proposed.  These studies will help define                    mechanisms of gene regulation both in vivo and in vitro.
{'FirstName': 'Shelley', 'LastName': 'Payne', 'PI_MID_INIT': 'M', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Shelley M Payne', 'EmailAddress': 'payne@mail.utexas.edu', 'NSF_ID': '000377795', 'StartDate': '05/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Texas at Austin', 'CityName': 'AUSTIN', 'ZipCode': '787121139', 'PhoneNumber': '5124716424', 'StreetAddress': '110 INNER CAMPUS DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '25', 'CONGRESS_DISTRICT_ORG': 'TX25', 'ORG_UEI_NUM': 'V6AFQPN18437', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF TEXAS AT AUSTIN', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '111200', 'Text': 'Genetic Mechanisms'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '914500', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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