Control of Genome Structure
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
The purpose of the proposed research is to characterize a                       novel genetic process in Neurospora crassa referred to by the                   acronym as "RIPing" (rearrangement induced premeiotically).                     Our preliminary work suggests that RIPing is responsible for                    rearranging certain duplicated DNA sequences at high                            frequency.  The process also typically leads to methylation                     of the affected sequences, de novo.  We will: (1) test the                      hypothesis that RIPing is triggered by sequence duplications;                   (2) determine the nature of the induced alterations; (3)                        explore why some sequences appear resistant to the process;                     (4) attempt to isolate mutants defective in RIPing: and (5)                     investigate whether RIPing is responsible for a class of                        spontaneous mutations.                                                                                                                                          Characterization of this process may illuminate unrecognized                    genetic mechanisms, and at the same time should provide                         information of practical use to scientists making                               modifications to the genome.  RIPing is almost certainly                        responsible for instability of transforming DNA in N. crassa,                   and it seems possible that a process analogous to RIPing is                     responsible for DNA rearrangements observed in other systems,                   such as in transgenic mice.  It is likely that an                               understanding of RIPing will also give us insights into why                     the genome is organized the way it is.
{'FirstName': 'Eric', 'LastName': 'Selker', 'PI_MID_INIT': 'U', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Eric U Selker', 'EmailAddress': 'selker@molbio.uoregon.edu', 'NSF_ID': '000429389', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Oregon Eugene', 'CityName': 'EUGENE', 'ZipCode': '974031905', 'PhoneNumber': '5413465131', 'StreetAddress': '1776 E 13TH AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Oregon', 'StateCode': 'OR', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'OR04', 'ORG_UEI_NUM': 'Z3FGN9MF92U2', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF OREGON', 'ORG_PRNT_UEI_NUM': 'Z3FGN9MF92U2'}
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[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '1116', 'Text': 'EUKARYOTIC GENETICS'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8718163.xml'}