Temporal Regulation of Baculovirus Gene Expression
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
The polyhedrin gene of Autographa californica nuclear                           polyhedrosis virus is maximally expressed very late in                          infection, after the release of extracellular virus.  It is                     believed that temporal regulation of polyhedrin requires the                    sequential expression of an immediate early gene(s) to                          activate delayed early gene expression; a delayed early                         gene(s) to turn on DNA synthesis and large gene expression;                     and a late gene(s) to stimulate polyhedrin, a very large                        gene.  I have devised a complementation assay to map the                        viral factors which control the temporal regulation of                          polyhedrin expression.  A plasmid, polcat, was constructed                      that contains the bacterial gene chloramphenicol                                acetyltransferase (CAT) under the control of the polyhedrin                     promoter.  CAT is expressed upon cotransfection of                              permissive insect cells with polcat and viral DNA, but not                      with viral DNA which has previously been digested with                          restriction enzymes.  The inability of digested DNA to                          support polcat expression is apparently due to inactivation                     of one or more essential genes by restriction digest.  The                      location of these genes can be mapped by complemenation with                    cloned PstI-restriction fragments.  Preliminary results                         indicated that twelve of the PstI clones complement different                   digests of viral DNA, indicating that at least twelve genes                     are involved in the temporal cascade. I intend to map and                       determine the function of several of these factors in                           temporal regulation of polyhedrin expression.                                                                                                                   The ultimate goal of this project is to define a limited set                    of viral genes which permits high level expression of                           polyhedrin in insect cells.  Although it may not be possible                    to identify all of the factors during the grant period, this                    research should increase our understanding of the mechanisms                    of temporal regulation of baculovirus gene expression.
{'FirstName': 'Linda', 'LastName': 'Guarino', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Linda A Guarino', 'EmailAddress': 'lguarino@tamu.edu', 'NSF_ID': '000417227', 'StartDate': '12/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Texas A&M Research Foundation', 'CityName': 'COLLEGE STATION', 'ZipCode': '778454375', 'PhoneNumber': '9798626777', 'StreetAddress': '400 HARVEY MITCHELL PKWY S STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Texas', 'StateCode': 'TX', 'CONGRESSDISTRICT': '10', 'CONGRESS_DISTRICT_ORG': 'TX10', 'ORG_UEI_NUM': 'EQH8NQ4AXFT7', 'ORG_LGL_BUS_NAME': 'TEXAS A & M RESEARCH FOUNDATION', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '111600', 'Text': 'EUKARYOTIC GENETICS'}, {'Code': '914500', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8804732.xml'}