Ribosomal RNA Processing: Removal of the Gap in Eukaryotic  26S-28S rRNA
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg'}
In general the largest mature derivative in the  processing                     of precursor 45S ribosomal RNA (rRNA) is 26S-28S rRNA, a                        component of the large ribosomal subunit.  Little is known                      about the structural elements, interacting molecules, and                       machinery needed to remove transcribed spacers from                             eukaryotic rRNA.  In some eukaryotic Z6S-Z8S rRNA is further                    fragmented into alpha and beta halves, held together by                         hydrogen bonding.  There is also a dearth of information                        about the phenomenon, which is called gap processing.  The                      long term goals of the project are to determine the                             recognition signals and mechanisms involved in gap processing                   and to ascertain its role in ribosome function.                                                                                                                 The specific objectives of this proposal are to:  1)                            determine the interaction of ribosomal protein L25 with the                     gap region in the fungus fly Sciara coprophila by in vitro                      binding experiments; 2)  determine the structure of rRNA in                     the gap region using strand-specific nucleases as probes and                    primer extension analysis; 3) localize the site of gap                          processing by subcellular fractionation of Tetrahymena; and                     4) determine the fate of the excised rRNA by hybridization                      and S1 nuclease or Northern blot analyses.  These studies                       should provide a better understanding of gap processing and                     the expression of mature rRNA in eukaroyotes.
{'FirstName': 'Vassie', 'LastName': 'Ware', 'EmailAddress': 'vcw0@lehigh.edu', 'StartDate': '07/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Lehigh University', 'CityName': 'Bethlehem', 'ZipCode': '180153005', 'PhoneNumber': '6107583021', 'StreetAddress': 'Alumni Building 27', 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA'}
[{'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1986&All=true', 'xml': '8620212.xml'}