Primary Amplification of ADH2 and ADH4 in the Yeast         Saccharomyces Cerevisiae
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
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The alcohol dehydrogenase (ADH) system of the yeast Saccharomyces               cerevisiae will be used to isolate ADH2 and ADH4 amplifications                 in a single selection step and estimate primary amplification                   rates for ADH2 and ADH4.  A duplication of ADH2 (Paquin and                     Williamson, unpublished data) and an amplification of ADH4                      (Walton, Paquin, Kaneko and Williamson, 1986) have been isolated.               The ADH system, in conjunction with the tools of genetics and                   molecular biology available in Saccharomyces cerevisiae, will                   provide a unique opportunity to investigate primary amplification               mechanisms at the single cell level in a simple, eukaryotic                     organism.                                                                                                                                                       An understanding of the mechanisms utilized for gene                            amplification is of obvious fundamental importance but there are                also practical ramifications.  There have been demonstrations                   that in some cases the acquisition of drug resistance is the                    result of gene duplication.  It is also true that in other                      examples, the phenotype of an organism is related to the number                 of gene copies.  Thus, insight into the mechanisms involved can                 suggest approaches to practical problems as well as increasing                  our understanding of genetic mechanisms.
{'FirstName': 'Charlotte', 'LastName': 'Paquin', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Charlotte E Paquin', 'EmailAddress': 'charlotte.paquin@uc.edu', 'NSF_ID': '000463870', 'StartDate': '08/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Cincinnati Main Campus', 'CityName': 'CINCINNATI', 'ZipCode': '452202872', 'PhoneNumber': '5135564358', 'StreetAddress': '2600 CLIFTON AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Ohio', 'StateCode': 'OH', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'OH01', 'ORG_UEI_NUM': 'DZ4YCZ3QSPR5', 'ORG_LGL_BUS_NAME': 'CINCINNATI UNIV OF', 'ORG_PRNT_UEI_NUM': 'DZ4YCZ3QSPR5'}
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[{'Code': '111200', 'Text': 'Genetic Mechanisms'}, {'Code': '111600', 'Text': 'EUKARYOTIC GENETICS'}]
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