Rayleigh Imaging of Macromolecular Motions in Living Cells
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
This research will apply a recently developed, ultrasensitive              interferometric imaging technique to map, onto images of a living cell,         the distribution of motion of its macromolecules.  The larger                   macromolecules can behave as optical markers even though they may be            spaced too closely to be optically resolved, and interference between           their scatterings of coherent incident light contains statistically rich        information about their relative disposition.  This information can be          extracted by quantitative, pixel-by-pixel analysis of the map                   intensities, and quantitative measures of the scales of length and time         over which their motions are correlated can be established even when the        spatial scale proves to be fine in comparison to an optical wavelength.         Finally, the structure and composition of skeletal muscle are such that         the force generators (crossbridges) themselves are its dominant Rayleigh        scatterers.  Interferometric Rayleigh maps of intact skeletal muscle            fibers developing steady tension are thus likely to yield information           that has an intimate and unusually direct connection with the scale on          which one of the Life Processes is organized.                                       This research represents a potentially major technical advance in           our ability to observe macromolecular movements in the living cell.  The        technique, once perfected, can probably be adapted to other problems of         regular movement at the macromolecular level, also.
{'FirstName': 'Mark', 'LastName': 'Sharnoff', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Mark Sharnoff', 'EmailAddress': None, 'NSF_ID': '000475416', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Delaware', 'CityName': 'NEWARK', 'ZipCode': '197160099', 'PhoneNumber': '3028312136', 'StreetAddress': '220 HULLIHEN HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Delaware', 'StateCode': 'DE', 'CONGRESSDISTRICT': '00', 'CONGRESS_DISTRICT_ORG': 'DE00', 'ORG_UEI_NUM': 'T72NHKM259N3', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF DELAWARE', 'ORG_PRNT_UEI_NUM': 'T72NHKM259N3'}
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[{'Code': '1108', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}, {'Code': '1114', 'Text': 'Cellular Dynamics and Function'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8821065.xml'}