Acquisition of Shared Spectropolarimeter for CD Studies
{'Code': '08080102', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DBI', 'LongName': 'Div Of Biological Infrastructure'}}
{'SignBlockName': 'Kathie L. Olsen'}
This proposal requests support to obtain powerful and highly                    sensitive CD spectroscopic equipment.  All of the projects                      involve the use of recombinant DNA technology to generate                       recombinant proteins (nerve growth factor), transcarboxylase                    subunits, glucokinase, and the retroviral nucleocapsid protein.                 We propose to determine the structure and function of these                     recombinant proteins and to compare them with the corresponding                 properties of the native protein; it is clear that the structural               properties must be evaluated before any functional changes can be               interpreted.  Such studies require the comparison of CD spectra                 from native and recombinant proteins, as well as functional                     studies.  Thus we require access to a spectropolarimeter capable                of high sensivity and low wavelength for quantitative evaluation                of small amounts of sample (frequently of the native protein).                  Sensitivity is also important for the monitoring of nucleocapsid                protein binding to nucleic acid by following the amplitude of the               CD band from the nucleic acid.  Currently access to the requested               CD instrumentation is unavailable on campus, because the resident               spectropolarimeter is out of date and inadequate.  The requested                instrument will be available to serve any other qualified user on               campus, time permitting.
[{'FirstName': 'Kenneth', 'LastName': 'Neet', 'EmailAddress': '2163683684', 'StartDate': '04/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Joyce', 'LastName': 'Jentoft', 'EmailAddress': 'jej@cwru.edu', 'StartDate': '04/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Harland', 'LastName': 'Wood', 'EmailAddress': None, 'StartDate': '04/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'Case Western Reserve University', 'CityName': 'CLEVELAND', 'ZipCode': '441064901', 'PhoneNumber': '2163684510', 'StreetAddress': 'Nord Hall, Suite 615', 'CountryName': 'United States', 'StateName': 'Ohio', 'StateCode': 'OH'}
{'Code': '1108', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8820739.xml'}