Site-Specific Mutation of Nitrogenase Proteins
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
Nitrogen fixation component enzymes have been isolated and                      crystallized.  The 3-dimensional structures will soon be known.                 The objective of this award is to the methods of side specific                  mutagenesis to augment biochemical and biophysical studies of                   the Fe-protein component.  The experiments are designed to                      elucidate: a) the role of the five conserved cysteines, and                     arginine-100 and b) the amino acid residues involved in complex                 formation between components of nitrogenase, flavodoxin binding                 and ATP binding.  The effects of substitutions will be studied                  by biochemical, biophysical and crystallographic methods.                                                                                                       The results will enhance our understanding of the structure and                 function of nitrogenase, an enzyme which converts molecular                     nitrogen into ammonia and makes it utilizable by all the forms                  of life on earth.
{'FirstName': 'James', 'LastName': 'Howard', 'PI_MID_INIT': 'B', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'James B Howard', 'EmailAddress': None, 'NSF_ID': '000294531', 'StartDate': '09/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Minnesota-Twin Cities', 'CityName': 'MINNEAPOLIS', 'ZipCode': '554552009', 'PhoneNumber': '6126245599', 'StreetAddress': '200 OAK ST SE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Minnesota', 'StateCode': 'MN', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MN05', 'ORG_UEI_NUM': 'KABJZBBJ4B54', 'ORG_LGL_BUS_NAME': 'REGENTS OF THE UNIVERSITY OF MINNESOTA', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8808943.xml'}