Polyene Antibiotics and Dictyostelium Development
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Kevin Thompson', 'PO_EMAI': 'kthompso@nsf.gov', 'PO_PHON': '7032924220'}
The main objective of this proposal is to understand the                   biological and biochemical basis of polyene antibiotic resistance                                                                                               and sensitivity.  A genetic-physiological framework will be                     established for the study of polyene-resistant mutants and the                  analysis of polyene resistance and sensitivity through the study                of these mutants.  The framework will be constructed by isolating                                                                                               and genetically characterizing polyene-resistant mutants,                       analyzing the mutants for sterol alterations by gas                             chromatographic and mass spectroscopic analysis, screening the                  mutants for polyene-sterol binding and for the state of polyene-                induced permeability.  Although most of the evidence suggests                   that polyene caused cell death is the result of cell leakage,                   recent evidence suggests that the products of polyene                           autoxidation may be responsible for polyene toxicity.  In his                   analysis of polyene-resistant mutants Dr. Katz has found a class                of mutants that appear to be deficient in cell-substratum                       adhesion, reduced in membrane sterol content and increased in                   cellular peroxisomal activity.  The relationship of these three                 results to each other and to polyene resistance and sensitivity                 will be further studied.                                                             Polyenes are widely used clinically important drugs whose                  mode of action depends on their interaction with membrane sterol.                                                                                               Sterols are major structural components of the plasma membrane                  whose function is largely unknown.  There is a growing body of                  literature that indicates that they may play an active role in                  cellular regulation rather than only passively modulating                       membrane fluidity.  Dictyostelium discoideum with its extensive                 developmental program which features such membrane processes as                 cell motility, adhesion and phagocytosis will be used for these                 studies.
{'FirstName': 'Eugene', 'LastName': 'Katz', 'PI_MID_INIT': 'R', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Eugene R Katz', 'EmailAddress': None, 'NSF_ID': '000337917', 'StartDate': '07/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'SUNY at Stony Brook', 'CityName': 'STONY BROOK', 'ZipCode': '117940001', 'PhoneNumber': '6316329949', 'StreetAddress': 'W5510 FRANKS MELVILLE MEMORIAL L', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'NY01', 'ORG_UEI_NUM': 'M746VC6XMNH9', 'ORG_LGL_BUS_NAME': 'THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1111', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8703045.xml'}