Higher Plant Nitrate Reductase: Structure, Mechanism and    Modification
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
Nitrate reductase (NR) is the enzyme that reduces soil                          nitrate to nitrite, a key step in the utilization of                            inorganic nitrogen fertilizer by plants.  This proposal is                      aimed at elucidating the structure and mechanism of action of                   NR, which has been purified to homogeneity using affinity                       chromatography and monoclonal antibody columns.  The NR gene                    from squash has been cloned.  Efforts will be made to express                   this gene in a suitable host so that NR can be obtained in                      quantity for crystallization studies.  The enzyme will be                       subjected to limited proteolysis and the fragments sequenced                    in order to identify the active sites.                                                                                                                          The results will enhance our understanding of how NR works,                     and may eventually enable plants to utilize nitrogen                            fertilizer more efficiently.
{'FirstName': 'Wilbur', 'LastName': 'Campbell', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Wilbur H Campbell', 'EmailAddress': 'wcampbel@mtu.edu', 'NSF_ID': '000448644', 'StartDate': '07/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Michigan Technological University', 'CityName': 'HOUGHTON', 'ZipCode': '499311200', 'PhoneNumber': '9064871885', 'StreetAddress': '1400 TOWNSEND DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Michigan', 'StateCode': 'MI', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'MI01', 'ORG_UEI_NUM': 'GKMSN3DA6P91', 'ORG_LGL_BUS_NAME': 'MICHIGAN TECHNOLOGICAL UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'GKMSN3DA6P91'}
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[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
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