Regulatory Mechanisms of Surface Protein Expression
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
The overall objective of this research is to understand how                     surface protein genes are regulated in response to a change                     in cell environment.  The ciliated protozoan, Tetrahymena                       thermophila is being used as a model system because it                          contains a family of surface protein genes (serotype genes)                     whose expression is dependent on incubation temperature.                        Specific aims of this proposal are:  l) Isolate and                             characterize Tetrahymena SerH3 genomic fragments by                             sequencing the flanking and coding regions and mapping                          transcription stop and start sites; 2) Identify regions of                      the SerH3 mRNA which are responsible for conferring                             temperature dependent expression by developing a cell free                      cytoplasmic extract and constructing SerH3 chimeric mRNAs; 3)                   Identify the specific trans-acting factors (positive or                         negative) responsible for SerH3 mRNA stability by using SerH3                   regulatory mutants and identifying the factor(s) which bind                     to SerH3 messages.                                                                                                                                              This research should provide insight into the mechanisms                        regulating genomic responses to environmental signals.
{'FirstName': 'Gary', 'LastName': 'Bannon', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Gary A Bannon', 'EmailAddress': 'bannongarya@exhange.usms.edu', 'NSF_ID': '000393055', 'StartDate': '04/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Arkansas Medical Sciences Campus', 'CityName': 'LITTLE ROCK', 'ZipCode': '722057101', 'PhoneNumber': '5016865502', 'StreetAddress': '4301 W MARKHAM ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Arkansas', 'StateCode': 'AR', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'AR02', 'ORG_UEI_NUM': 'VDFYLZPJEAV6', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES', 'ORG_PRNT_UEI_NUM': None}
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