Techniques of Flow Cytoenzymological Analysis
{'Code': '08000000', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}}
{'SignBlockName': 'Machi F. Dilworth', 'PO_EMAI': None, 'PO_PHON': None}
This proposal requests funds for the development of novel flow                  cytometric instrumentation and operating procedures for use in                  the cytoenzymological analysis of large eukaryotic cells, those                 cells having diameters in the range of 20-l20 um.  The research                 focuses on the development of novel substrates for use in flow                  cytoenzymology and on the development of techniques to permit the               real-time analysis and sorting of cells based on surface area,                  volume, and concentration.  The techniques will be employed for                 the analysis of those factors involved in the expression in                     plants of suitably engineered chimaeric genes, specifically beta-               glucuronidase, following transfer from bacteria, and for the                    selection through flow sorting of living plant protoplasts                      according to the level of expression of the introduced genes.
{'FirstName': 'David', 'LastName': 'Galbraith', 'PI_MID_INIT': 'W', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David W Galbraith', 'EmailAddress': 'galbraith@arizona.edu', 'NSF_ID': '000472027', 'StartDate': '11/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Nebraska-Lincoln', 'CityName': 'LINCOLN', 'ZipCode': '685032427', 'PhoneNumber': '4024723171', 'StreetAddress': '2200 VINE ST # 830861', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Nebraska', 'StateCode': 'NE', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'NE01', 'ORG_UEI_NUM': 'HTQ6K6NJFHA6', 'ORG_LGL_BUS_NAME': 'BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '110800', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8709697.xml'}