Messenger RNA Processing in Spinach Chloroplasts
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Kamal Shukla', 'PO_EMAI': None, 'PO_PHON': None}
The longterm goal of this research is to discover how gene                      expression in chloroplasts affects and is itself affected by                    chloroplast development and biogenesis.  This goal will be                      approached by first studying the processing of multigenic                       messenger RNAs transcribed within the chloroplast.                                                                                                              There are two main objectives to this project.  First is to                     identify precursor and product RNAs from the ATP synthase                       gene cluster in spinach chloroplasts.  In the course of                         achieving that goal, the primary transcripts from that gene                     cluster will be identified.  The second objective is to                         observe the levels of these multigenic transcripts and their                    processing products as the chloroplast develops from its                        immature beginnings in the seedling stage to the fully                          developed state that it reaches in the mature leaf.  Both of                    these objectives will be studied with a number of molecular                     biological techniques, the chief among them being Northern                      analysis.%%%                                                                                                                                                    Chloroplasts are the cell organelles where photosynthesis                       takes place.  Chloroplasts, like the cell nucleus, have genes                   which direct the synthesis of proteins needed for                               photosynthesis.  mRNA's are the intermediates between genes                     and proteins.  There has been very little research performed                    on mRNA processing in chloroplasts.  Results from this                          research will serve as the first steps in the elucidation of                    this complex process.***                                                         //
{'FirstName': 'Margaret', 'LastName': 'Hollingsworth', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Margaret J Hollingsworth', 'EmailAddress': 'hollings@buffalo.edu', 'NSF_ID': '000362927', 'StartDate': '09/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'SUNY College at Buffalo', 'CityName': 'BUFFALO', 'ZipCode': '142221004', 'PhoneNumber': '7168783047', 'StreetAddress': '1300 ELMWOOD AVE', 'StreetAddress2': 'BUCKHAM HALL B206', 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '26', 'CONGRESS_DISTRICT_ORG': 'NY26', 'ORG_UEI_NUM': 'VKAPREANC226', 'ORG_LGL_BUS_NAME': 'THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1144', 'Text': 'Molecular Biophysics'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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