Physical Studies of the Translocatable Genetic Elements in  Bacteria
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
We will continue our isolation and characterization of                          mutations that affect transposase expression at steps                           subsequent to transcription initiation in order to determine                    why translation of the transposase gene is so rare, to assess                   the interplay between translation and messenger degradation,                    and to determine the roles of these events in IS10 anti-sense                   inhibition.  We will analyze bacterium E.coli mutant strains                    lacking IHF and/or HU to determine the relative contributions                   of these, and any other related accessory host factors present                  in wild type cells, to the activity of IS10 outside ends and to                 host factor inhibition of transposase expression in vivo.  We                   will also try to understand why E.coli uses IHF to regulate                     IS10 transposition (and other processes) by examining changes                   in IHF levels under different growth conditions.  Finally,                      using strains lacking known host factors, we will isolate                       revertants in which outside end activity is restored in hopes                   of identifying genes for additional accessory proteins.                         Depending on the progress made in (1) and (2), we may initiate                  a new search for E.coli mutants defective in the transposition                  reaction.
{'FirstName': 'Nancy', 'LastName': 'Kleckner', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Nancy E Kleckner', 'EmailAddress': 'kleckner@fas.harvard.edu', 'NSF_ID': '000463553', 'StartDate': '04/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Harvard University', 'CityName': 'CAMBRIDGE', 'ZipCode': '021385366', 'PhoneNumber': '6174955501', 'StreetAddress': '1033 MASSACHUSETTS AVE STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'LN53LCFJFL45', 'ORG_LGL_BUS_NAME': 'PRESIDENT AND FELLOWS OF HARVARD COLLEGE', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '1156', 'Text': 'MICROBIAL GENETICS'}]
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