DNA Synthesis in Rice Endosperm
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Kevin Thompson', 'PO_EMAI': 'kthompso@nsf.gov', 'PO_PHON': '7032924220'}
The DNA content of rice endosperm nuclei, instead of remaining at               the expected 3C level, increases to higher values.  The questions               that are being asked are the following.  What is the pattern of                 DNA synthesis and accumulation in endosperm nuclei during the                   entire course of development of this tissue?  If the endosperm                  nuclei exhibits a modal increase in DNA content, is this                        referable to cell fusion, polyploidy, polyteny, endoreduplication               or selective gene amplification?  Is there evidence for the                     under.representation of certain genes during the ontogeny of the                endosperm?  Autoradiographic (including DNA fiber                               autoradiography) and Feulgen microspectrophotometic methods will                be used to study the changing pattern of DNA synthesis and                      accumulation.  The reassociation kinetics of endosperm DNA with                 leaf or embryo DNA and with cloned genes will be employed to                    establish whether the high DNA content is due to                                endoreduplication or gene amplification.  The kinetics of                       hybridization between endosperm DNA and rRNA will be analyzed to                determine whether the rRNA genes are under.replicated during                    endosperm development.
{'FirstName': 'Valayamghat', 'LastName': 'Raghavan', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Valayamghat Raghavan', 'EmailAddress': None, 'NSF_ID': '000099702', 'StartDate': '09/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Ohio State University Research Foundation -DO NOT USE', 'CityName': 'Columbus', 'ZipCode': '432101016', 'PhoneNumber': '6146888734', 'StreetAddress': '1960 KENNY RD', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Ohio', 'StateCode': 'OH', 'CONGRESSDISTRICT': '03', 'CONGRESS_DISTRICT_ORG': 'OH03', 'ORG_UEI_NUM': 'QR7NH79713E5', 'ORG_LGL_BUS_NAME': None, 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1111', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8709092.xml'}