Physical Chemical Basis of Control of Gene Expression
{'Code': '03090007', 'Directorate': {'Abbreviation': 'MPS', 'LongName': 'Direct For Mathematical & Physical Scien'}, 'Division': {'Abbreviation': 'CHE', 'LongName': 'Division Of Chemistry'}}
{'SignBlockName': 'Richard Hilderbrandt', 'PO_EMAI': None, 'PO_PHON': None}
This project is in the Chemistry of Life Processes Initiative                   in the Office of Special Projects in Chemistry.  The objectives                 of this research are to understand the physical chemical                        foundations of control of gene expression in living systems.                    The general thermodynamic principles governing stability and                    specificity of regulatory proteins and nucleic acids, and the                   important steps involved in the association and dissociation of                 these macromolecular species, are now reasonably understood in                  dilute solution under laboratory conditions.  This project will                 extend the this level of chemical understanding to the crowded                  environment of the cell cytoplasm.  This fundamental research                   will have biomedical and biotechnological significance.                                                                                                         Experiments will quantify a) the chemical environment of the                    cytoplasm of E. coli and the variability of this environment,                   b) the properties of and structures of DNA in this environment,                 and c) the effects of the cytoplasmic environment on                            noncovalent interactions of proteins and nucleic acids.  In                     particular the project wil address the origins of the in                        vivo-in vitro paradox in which protein-DNA interactions are                     highly salt dependent in vitro and salt independent in vivo.                    This project is a renewal of one previously supported by the                    Foundation.
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[{'Code': '1956', 'Text': 'STATISTICAL AND SIMULATIONS'}, {'Code': '1982', 'Text': 'BIOLOGICAL CHEMISTRY'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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