Membrane Traffic and Topography
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
Intracellular membrane traffic in cells has been postulated                to require small vesicles which shuttle between fixed                           endomembrane compartments of the cell.  Such vesicles are thought               to carry contents in a vectorial manner and return membrane                     material to the point of origin after delivery.  Though often                   postulated, little morphological evidence has been forthcoming to               show that these are real. Cryoelectron microscopy will be                       utilized to attempt to visualize such vesicles in Paramecium.                   Cryofixation will be used to physically fix cells ultrarapidly so               intermediate steps in fission or fusion can be preserved.                       Subsequent cryosubstitution or deep-etch rotary-shadow steps will               allow observation of the cell interior in a nearly native state,                making it possible to locate small transport or shuttle                         vesicles.  Monoclonal antibody histochemistry will be used to                   identify membrane components of the shuttle vesicles.                                                                                                           These studies will provide new information on the movements of                  membranes and membrane components within cells.
[{'FirstName': 'Agnes', 'LastName': 'Fok', 'PI_MID_INIT': 'K', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Agnes K Fok', 'EmailAddress': 'fok@hawaii.edu', 'NSF_ID': '000411931', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Richard', 'LastName': 'Allen', 'PI_MID_INIT': 'D', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Richard D Allen', 'EmailAddress': 'allen@pbrc.hawaii.edu', 'NSF_ID': '000388267', 'StartDate': '02/15/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'University of Hawaii', 'CityName': 'HONOLULU', 'ZipCode': '968222247', 'PhoneNumber': '8089567800', 'StreetAddress': '2425 CAMPUS RD SINCLAIR RM 1', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Hawaii', 'StateCode': 'HI', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'HI01', 'ORG_UEI_NUM': 'NSCKLFSSABF2', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF HAWAII', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
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