Segregation of Developmentally Restricted Subpopulations in the Neural Crest Lineage
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith A. Verbeke', 'PO_EMAI': None, 'PO_PHON': None}
Dr. Weston will exploit the avian neural crest, which provides an               experimentally accessible population of pluripotent embryonic                   cells that undergoes a characteristic sequence of restrictions of               developmental potential.  Phenotypically distinct crest-derived                 subpopulations can be detected at early stages of development                   using sensitive cell type-specific markers.  Some of these                      subpopulations appear to arise from developmentally restricted,                 but functionally undifferentiated embryonic cells.  Although the                existence of developmentally restricted precursors has been                     inferred, they cannot now be identified directly.  Therefore to                 characterize the initial appearance and ultimate fate of these                  cells, we propose: 1) to identify subpopulations of crest-derived               cells that have distinct, limited developmental potentials using                specifically identified monoclonal antibody markers; and 2) to                  characterize the normal developmental appearance of                             immunochemically identified cells, and the subsequent expression                by these cells of other phenotypic traits characteristic of crest               derivatives.  To examine early restriction of developmental                     potential, we propose; 3) to analyze the response of identified                 precursors to environmental factors, including an assessment of                 a) the responses of a crest-derived neurogenic precursor                        subpopulation to specific growth and survival factors, and b) the               normal responses of nascent neurons to environmental cues.                      %%%                                                                             The developmental potential of embryonic cells becomes                          progressively more restricted during embryogenesis. The                         mechanisms by which this occurs remain to be elucidated. In this                study Dr. Weston will address the important issue of how this                   diversity is generated, stabilized and propagated within cellular               lineages during embryonic development.
[{'FirstName': 'James', 'LastName': 'Weston', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'James A Weston', 'EmailAddress': '5036865187', 'NSF_ID': '000241776', 'StartDate': '05/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Michael', 'LastName': 'Marusich', 'PI_MID_INIT': 'F', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Michael F Marusich', 'EmailAddress': None, 'NSF_ID': '000328421', 'StartDate': '05/01/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'University of Oregon Eugene', 'CityName': 'EUGENE', 'ZipCode': '974031905', 'PhoneNumber': '5413465131', 'StreetAddress': '1776 E 13TH AVE', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Oregon', 'StateCode': 'OR', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'OR04', 'ORG_UEI_NUM': 'Z3FGN9MF92U2', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF OREGON', 'ORG_PRNT_UEI_NUM': 'Z3FGN9MF92U2'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1111', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8820070.xml'}