The Tobacco Ringspot Virus and its Associated Satellite     in Plant Protoplasts Infected by Electroporation
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
Some RNA plant viruses become associated with RNAs termed                       "small satellite RNAs".  The ca. 360 nucleotide residue                         satellite RNA of tobacco of tobacco ringspot virus (STobRV RNA)                 is not a messenger RNA, and it has no extensive nucleotide                      sequence homology with its supporting virus, tobacco ringspot                   virus (TobRV), as detected by hybridization analysis.                           Nevertheless, STobRV RNA replicates in TobRV infected plants                    where it reduces TobRV accumulation and symptom severity and                    becomes encapsidated in TobRV coat protein.  Multimeric and                     circular forms of STobRV RNA found in infected tissues are                      likely replication intermediates.  The goals of this study are                  to develop electroporation procedures to infect plant cell                      protoplasts with TobRV and STobRV and to study the mechanism of                 replication of the satellite RNA in protoplasts.  These studies                 will allow elucidation of the interactions and relationships                    between the genomic and satellite RNAs in this system and will                  enable these relationships in protoplasts to be compared with                   those of intact plants.
{'FirstName': 'Gerry', 'LastName': 'Prody', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Gerry A Prody', 'EmailAddress': 'prody@chem.wwu.edu', 'NSF_ID': '000456566', 'StartDate': '05/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Western Washington University', 'CityName': 'BELLINGHAM', 'ZipCode': '982255996', 'PhoneNumber': '3606502884', 'StreetAddress': '516 HIGH ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Washington', 'StateCode': 'WA', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'WA02', 'ORG_UEI_NUM': 'U3ZFA57417D4', 'ORG_LGL_BUS_NAME': 'WESTERN WASHINGTON UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'U3ZFA57417D4'}
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