Fiber Optic Biosensors for Glutamate
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
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Glutamate biosensing probes based on fiber optic technology will                be developed.  These probes consist of the enzyme glutamate                     dehydrogenase immobilized at the distill tip of an optical fiber.               This enzyme catalyzes the reaction between glutamate and oxidized               nicotinamide adenine dinucleotide to form 2-oxoglutarate, ammonia               and reduced nicotinamide adenine dinucleotide (NADH).  The                      generation of NADH is monitored by either a fluorescence or                     bioluminescence process.  In both cases, a steady-state signal is               produced which can be related to the sample concentration of                    glutamate.  If successful, the proposed glutamate biosensors will               be suitable for in vivo studies to determine the neurological                   properties of glutamate.  These sensors will allow localized,                   intratissue measurements of glutamate (and possibly aspartate) in               intact neuoral tissue.  Such localized measurements can                         potentially separate synaptic from non-synaptic pools of these                  putative neurotransmitters.  In addition, a reagent-less                        glutamate probe will also be investigate as a means to make                     continuous, in vivo glutamate measurements.
{'FirstName': 'Mark', 'LastName': 'Arnold', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Mark A Arnold', 'EmailAddress': 'mark-arnold@uiowa.edu', 'NSF_ID': '000287252', 'StartDate': '05/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Iowa', 'CityName': 'IOWA CITY', 'ZipCode': '522421316', 'PhoneNumber': '3193352123', 'StreetAddress': '105 JESSUP HALL', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Iowa', 'StateCode': 'IA', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'IA01', 'ORG_UEI_NUM': 'Z1H9VJS8NG16', 'ORG_LGL_BUS_NAME': 'THE UNIVERSITY OF IOWA', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1192', 'Text': 'NEURONAL AND GLIAL MECHANISMS'}, {'Code': '1974', 'Text': 'ANALYTICAL SEPARATIONS & MEAS.'}]
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