Molecular Studies of Signal Peptidase Function in Yeast
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
Signal peptidases are the key enzymes which are responsible                for removing the transient amino-terminal "signal peptides" which               direct cellular proteins across specific membrane barriers.  The                signal peptidase to be studied in this project is the one which                 cleaves the signal peptide from nascent secretory or membrane                   proteins which are directed through the rough endoplasmic                       reticulum membrane.  Yeast signal peptidase will be purified and                characterized, and proteins associated with signal peptidase                    function will be isolated.  Genes encoding these proteins will be               cloned and used in functional studies in vivo.  The role of the                 sec11 gene product (sec 11 is one of a series of yeast mutants                  with impaired secretory function) in signal peptide function will               be investigated.                                                                                                                                                This research will increase our understanding of the molecular                  basis of intracellular protein transport.  This will lead not                   only to an enhanced understanding of fundamental life processes                 at the cellular level, but may also have practical applications                 in biotechnology, particularly in the production of genetically-                engineered fermentation products.
{'FirstName': 'Peter', 'LastName': 'Bohni', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Peter C Bohni', 'EmailAddress': None, 'NSF_ID': '000446884', 'StartDate': '07/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'SUNY at Stony Brook', 'CityName': 'STONY BROOK', 'ZipCode': '117940001', 'PhoneNumber': '6316329949', 'StreetAddress': 'W5510 FRANKS MELVILLE MEMORIAL L', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'NY01', 'ORG_UEI_NUM': 'M746VC6XMNH9', 'ORG_LGL_BUS_NAME': 'THE RESEARCH FOUNDATION FOR THE STATE UNIVERSITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8819149.xml'}