Efficient Replication of DNA Injected into Xenopus Eggs
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser', 'PO_EMAI': None, 'PO_PHON': None}
We are investigating the molecular mechanisms controlling                       initiation of DNA replication in eggs of the frog Xenopus                       laevis.  Our approach makes use of new techniques and                           findings established in this laboratory, these include: 1)                      Development of a technique which permits injection of                           materials into eggs without activating them;  2) Discovery                      that injected Type 1 Bovine Papilloma Virus (BPV1) DNA is                       only replicated after eggs are activated, but replication is                    made more efficient by injection prior to activation; 3)                        Observation that vectors which lack the BPV1 origin of                          replication do not display the same improvement in                              replication efficiency.  These results provide the first                        clear indication that Xenopus eggs initiate DNA replication                     via use of a known eukaryotic origin.  Experiments aimed at                     further characterizing the egg-plasmid interactions are                         proposed: A) Determination of the optimal amount of DNA/egg                     which yields synchronous initiation of 1st round replication                    of the maximum number of molecules: B) Confirmation that                        double stranded DNA is replicated in activated eggs via a                       semiconservative mechanism; C) Determination, of the role of                    RNA transcription in the initiation of DNA replication.  D)                     Determination of whether plasmids which lack origins of                         replication inhibit the efficient replication of those which                    contain functional origins.  Experiments along these lines                      will help to test our working model which postulates that the                   protein factors in the cytoplasm of the unactivated egg                         interact with origin containing DNA sequences.                                                                                                                  The successful completion of these experiments will allow                       greater insight into the mechanisms and cellular factors                        necessary for the faithful initiation and replication of                        eukaryotic DNA in in vivo conditions.
{'FirstName': 'Lawrence', 'LastName': 'Wangh', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Lawrence J Wangh', 'EmailAddress': 'wangh@binah.cc.brandeis.edu', 'NSF_ID': '000178251', 'StartDate': '02/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Brandeis University', 'CityName': 'WALTHAM', 'ZipCode': '024532728', 'PhoneNumber': '7817362121', 'StreetAddress': '415 SOUTH ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'MXLZGAMFEKN5', 'ORG_LGL_BUS_NAME': 'BRANDEIS UNIVERSITY', 'ORG_PRNT_UEI_NUM': 'JLC9RHNT9479'}
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[{'Code': '1116', 'Text': 'EUKARYOTIC GENETICS'}, {'Code': '9120', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}]
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