Biochemical Studies of Protein Methylatin
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
The purpose of this award is to elucidate the structure-                        function relationships of the S. typhimurium CheR S-                            adenosylmethionine:glutamyl methyltransferase, an enzyme that                   is essential for the regulation of the membrane receptor                        proteins involved in chemotaxis.  An efficient CheR expression                  vector will be constructed so the enzyme can be over-produced                   and large amounts of it purified.  Efforts will be made to                      obtain good quality crystals in order to determine the                          structure of the protein X-ray analysis.  Attempts will be made                 to determine the identities of the amino acids that constitute                  the S-adenosyl- methionine binding site.  Modification                          reagents and inhibitors will be used to label active site                       resides which can then be examined using established methods                    of biochemical analysis.                                                                                                                                        The proposed research will help us understand how cells                         respond to chemical stimuli.  It may also develop specific                      inhibitors which may be useful in controlling gene expression                   and cell growth.
{'FirstName': 'Simon', 'LastName': 'Simms', 'PI_MID_INIT': None, 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Simon Simms', 'EmailAddress': 'simms@sci.ccny.cuny.edu', 'NSF_ID': '000172054', 'StartDate': '08/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'CUNY City University of New York', 'CityName': 'NEW YORK', 'ZipCode': '100175773', 'PhoneNumber': '6466648910', 'StreetAddress': '217 E 42ND ST', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'New York', 'StateCode': 'NY', 'CONGRESSDISTRICT': '12', 'CONGRESS_DISTRICT_ORG': 'NY12', 'ORG_UEI_NUM': 'UJ36PVKE4RY9', 'ORG_LGL_BUS_NAME': 'RESEARCH FOUNDATION OF THE CITY UNIVERSITY OF NEW YORK', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '116600', 'Text': 'MOLECULAR BIOCHEMISTRY'}, {'Code': '912000', 'Text': 'CROSS-DIRECTORATE PROGRAMS'}, {'Code': '914500', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8800876.xml'}