Molecular Analysis of a "Cadherin Related Molecule"
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith Plesset', 'PO_EMAI': None, 'PO_PHON': None}
All embryonic cells thus far examined bear both calcium                         independent and calcium dependent adhesive systems.  The calcium                dependent systems are mediated by a family of molecules referred                to generically as calCAMs.  Dr. Lilien has shown that the calCAMs               are divisible into at least two structural types on the basis of                their two dimensional peptide maps.  One group of calCAMs have                  peptide maps with over 60% of the spots being similar to N-                     cadherin related molecules.  The second type is represented by L-               CAM and shares only 20% of its peptides with the first group.                   Embryonic chick liver has both a cadherin related molecule, CRM-                L and L-CAM.  Dr. Lilien proposes to further compare the                        structure of CRM-L with N-cadherin and L-CAM.  To accomplish this               he will use molecular genetic techniques isolating cDNA clones of               the cadherin related molecules in liver.  He will screen liver                  cDNA libraries with antibodies recognizing all cadherins and                    oligonucleotide probes based on the known sequences of L-CAM and                N-cadherin. Additionally he will attempt to prepare antibodies                  which are specific to CRM-L.  To generate these he will use                     synthetic peptides chosen from sequence information for                         immunization of rabbits.  These antibodies along with monoclonals               will be screened immunohistochemically and ultimately analyzed                  for their effect on liver cell adhesion.                                        %%%                                                                             The development of the organs in an embryo involves movements of                cells and groups of cells relative to each other.  These                        movements require that cells make and break adhesions with                      neighboring cells.  This proposal is an investigation of a class                of molecules potentially important for adhesive interactions                    between cells.
{'FirstName': 'Jack', 'LastName': 'Lilien', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Jack E Lilien', 'EmailAddress': 'jlilien@biology.biosci.wayne.edu', 'NSF_ID': '000468155', 'StartDate': '07/15/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Wisconsin-Madison', 'CityName': 'MADISON', 'ZipCode': '537151218', 'PhoneNumber': '6082623822', 'StreetAddress': '21 North Park Street', 'StreetAddress2': 'Suite 6301', 'CountryName': 'United States', 'StateName': 'Wisconsin', 'StateCode': 'WI', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'WI02', 'ORG_DUNS_NUM': '161202122', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF WISCONSIN SYSTEM', 'ORG_PRNT_DUNS_NUM': '041188822'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1111', 'Text': 'DEVELOPMENTAL SYSTEMS CLUSTER'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8801909.xml'}