Receptor-Mediated Cell Separations
{'Code': '07020000', 'Directorate': {'Abbreviation': 'ENG', 'LongName': 'Directorate For Engineering'}, 'Division': {'Abbreviation': 'CBET', 'LongName': 'Div Of Chem, Bioeng, Env, & Transp Sys'}}
{'SignBlockName': 'Fred G. Heineken', 'PO_EMAI': None, 'PO_PHON': None}
The exploitation of cell surface receptors for selective cell                   separation processes holds great promise for a wide range of                    applications.  The fundamental engineering principles and                       underlying biochemical/biophysical phenomena to be generated by  the            proposed projects are expected to be generalizable to a very  diverse           range of potential cell separation processes.                                                                                                                   In addition to intrinsic receptor/ligand (binding molecule)                     biochemical interactions, there are a number of biophysical                     considerations which affect the selectivity and yield of                        receptor-mediated cell separation processes.  The PIs propose a set of          complimentary projects aimed at a quantitative understanding of the             influence of key parameters on receptor-mediated adhesion of cells to a         ligand-coated surface.  Specifically, they will investigate the affects         of receptor and ligand densities, receptor mobility, receptor/ligand            binding affinity, temperature, and fluid shear rate on receptor-mediated        cell separations.  Model systems will include antigen-coated polymeric          microspheres and cultured neuroblastome cells, with appropriate antibody        and lectin ligand-coated surfaces.                                                                                                                              This grant application is an excellent example of the type of                   cross-disciplinary proposal we wish to see in the Engineering                   Directorate's Biotechnology Program.  The Principal Investigators are           considered well qualified to carry out the proposed research and I              recommend funding of this proposal for three years in accordance with           the revised budget.
[{'FirstName': 'Douglas', 'LastName': 'Lauffenburger', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Douglas A Lauffenburger', 'EmailAddress': 'lauffen@mit.edu', 'NSF_ID': '000308106', 'StartDate': '11/01/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'John', 'LastName': 'Quinn', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'John A Quinn', 'EmailAddress': 'quinn@cheme.seas.upenn.edu', 'NSF_ID': '000302305', 'StartDate': '11/01/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'David', 'LastName': 'Graves', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David J Graves', 'EmailAddress': 'graves@seas.upenn.edu', 'NSF_ID': '000273646', 'StartDate': '11/01/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'University of Pennsylvania', 'CityName': 'Philadelphia', 'ZipCode': '191046205', 'PhoneNumber': '2158987293', 'StreetAddress': 'Research Services', 'StreetAddress2': '3451 Walnut St, 5th Flr Franklin', 'CountryName': 'United States', 'StateName': 'Pennsylvania', 'StateCode': 'PA', 'CONGRESSDISTRICT': '03', 'CONGRESS_DISTRICT_ORG': 'PA03', 'ORG_DUNS_NUM': '042250712', 'ORG_LGL_BUS_NAME': 'TRUSTEES OF THE UNIVERSITY OF PENNSYLVANIA, THE', 'ORG_PRNT_DUNS_NUM': '042250712'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1491', 'Text': 'Cellular & Biochem Engineering'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8712784.xml'}