Ribozyme Substrates
{'Code': '08070400', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Marcia Steinberg', 'PO_EMAI': None, 'PO_PHON': None}
This award will support research on two aspects of RNA                          catalysis.  The first is to define the substrate specificity of                 P RNA, the catalytic component of B. subtilis tRNA processing                   enzyme RNaseP.  Carefully designed mutations in the established                 domain of yeast tRNA-phe precursor will be assayed in the RNA                   dependent cleavage reaction.  Fluorescent assays will be                        developed to identify the binding and cleavage steps.  The                      second is to ascertain the mechanism of Pb and Mg dependent                     cleavage of yeast tRNA-phe.  Mutant tRNAs with unique deoxy-2-                  O'methyl and alpha-thio-phosphate substitutions will be                         prepared to test a model of Klug derived from X-ray diffraction                 data.                                                                                                                                                           The results will greatly aid our understanding of how RNA                       catalysis takes place, the evolution of enzymes and the origin                  of life on earth.
{'FirstName': 'Olke', 'LastName': 'Uhlenbeck', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Olke C Uhlenbeck', 'EmailAddress': 'o-uhlenbeck@northwestern.edu', 'NSF_ID': '000326521', 'StartDate': '08/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'University of Colorado at Boulder', 'CityName': 'Boulder', 'ZipCode': '803090001', 'PhoneNumber': '3034926221', 'StreetAddress': '3100 MARINE ST', 'StreetAddress2': 'STE 481 572 UCB', 'CountryName': 'United States', 'StateName': 'Colorado', 'StateCode': 'CO', 'CONGRESSDISTRICT': '02', 'CONGRESS_DISTRICT_ORG': 'CO02', 'ORG_UEI_NUM': 'SPVKK1RC2MZ3', 'ORG_LGL_BUS_NAME': 'THE REGENTS OF THE UNIVERSITY OF COLORADO', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1144', 'Text': 'Molecular Biophysics'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8805690.xml'}