Acquisition of a Transmission Electron Microscope
{'Code': '08080102', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DBI', 'LongName': 'Div Of Biological Infrastructure'}}
{'SignBlockName': 'Kathie L. Olsen', 'PO_EMAI': None, 'PO_PHON': None}
Matching funds have been requested by California State University               Long Beach for a transmission electron microscope (TEM).  The                   instrument will be used by a number of investigators within the                 School of Natural Sciences for a diverse range of projects                      requiring high resolution bright and dark field imaging and                     selected area diffraction.  These projects include studying the                 cytological dedifferentiation of primary invertebrate cells                     cultured on support beads, studying the formation and secretion                 of haemocyanin and inorganic polyphosphate inclusions in cultured               molluscan cells, examining the ability of various purified                      intermediate filament proteins to be reconstituted into                         filaments in vivo and examining the size and geometry of                        purified, soluble neuroprotein oligomers.  Other projects                       requiring conventional TEM include a study to quantitatively                    determine plasmodesmatal and desmotubule communication to                       estimate the relative degree of involvement of symplastic and                   apoplastic transport of photosynthates to sieve tubes.  The                     instrument will also be used to study the developmental changes                 in the cysts and trophozoite forms of Pneumocystis carinii and                  establish the mode of attachment of the organism to host lung                   epithelial cells.  A number of investigators require the                        instrument for immunocytological studies.  These studies aim to                 determine the induction and cytological distribution of                         metallothionein and heat shock 70 proteins in marine                            invertebrates upon exposure to environmental stress.  Other                     studies include the immunolocalization of phosphoprotein                        phosphatase-1 activating kinase and the various phosphatases and                their regulatory subunits in the enzyme cascade involved in                     glycogen metabolism.  TEM in combination with O2 plasma                         microincineration and energy dispersive X-ray microanalysis will                also be used to determine the ultrastructural features and                      cellular ligands involved in metal accumulation in commercially                 and ecologically important marine invertebrates.
[{'FirstName': 'Andrew', 'LastName': 'Mason', 'PI_MID_INIT': 'Z', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Andrew Z Mason', 'EmailAddress': None, 'NSF_ID': '000451573', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'Carol', 'LastName': 'Itatani', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Carol A Itatani', 'EmailAddress': None, 'NSF_ID': '000187464', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Frank', 'LastName': 'Alfieri', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Frank J Alfieri', 'EmailAddress': None, 'NSF_ID': '000459165', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Kenneth', 'LastName': 'Jenkins', 'PI_MID_INIT': 'D', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Kenneth D Jenkins', 'EmailAddress': None, 'NSF_ID': '000321654', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Jeffrey', 'LastName': 'Cohlberg', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Jeffrey A Cohlberg', 'EmailAddress': 'cohlberg@csulb.edu', 'NSF_ID': '000210793', 'StartDate': '04/01/1989', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
{'Name': 'California State University-Long Beach', 'CityName': 'LONG BEACH', 'ZipCode': '908154670', 'PhoneNumber': '5629855314', 'StreetAddress': '6300 E STATE UNIVERSITY DR STE 3', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '42', 'CONGRESS_DISTRICT_ORG': 'CA42', 'ORG_UEI_NUM': 'P2TDH1JCJD31', 'ORG_LGL_BUS_NAME': 'CALIFORNIA STATE UNIVERSITY LONG BEACH RESEARCH FOUNDATION', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '110800', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8820774.xml'}