Regulation of the Metabolic Processes of the single cell at the Molecular level
{'Code': '08070100', 'Directorate': {'LongName': 'Direct For Biological Sciences'}, 'Division': {'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'DeLill Nasser'}
The object of the proposed research is to elucidate the                         mechanism responsible for the activation of transcription at                    the nitrogen-regulated promoter glnAp2 of the structural gene                   for glutamine synthetase in Escherichia coli.  We have shown                    that core RNA polymerase with sigma54, the specific o factor                    for nitrogen regulated promoters, combines with glnAp2 in a                     closed complex.  The activator NRI, after phosphorylation by                    the modulator NRII stimulates the isomerization to the open                     complex.  We plan to separate the open complexes from other                     proteins and small molecules and to probe the interaction of                    NRI- phosphate with the DNA of the promoter and with sigma54.                   We shall also attempt to discover the mechanism responsible for                 the ability of NRI to activate in intact cells the                              transcription of glnAp2 in the absence of NRII by the isolation                 and study of mutants altered in this ability.  We shall attempt                 to identify the DNA sites in the vicinity of glnAp2 that are                    responsible for the dampening of glnAp2 transcription by an                     increased intracellular concentration of NRI.
{'FirstName': 'Boris', 'LastName': 'Magasanik', 'EmailAddress': 'bmag@mit.edu', 'StartDate': '06/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Massachusetts Institute of Technology', 'CityName': 'Cambridge', 'ZipCode': '021394301', 'PhoneNumber': '6172531000', 'StreetAddress': '77 MASSACHUSETTS AVE', 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA'}
{'Code': '1156', 'Text': 'MICROBIAL GENETICS'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8817091.xml'}