Structural and Functional Characteristics of Tubulin        Isoforms
{'Code': '03090007', 'Directorate': {'Abbreviation': 'MPS', 'LongName': 'Direct For Mathematical & Physical Scien'}, 'Division': {'Abbreviation': 'CHE', 'LongName': 'Division Of Chemistry'}}
{'SignBlockName': 'George M. Rubottom', 'PO_EMAI': None, 'PO_PHON': None}
This project will be supported by a joint award from the                   Chemistry Office of Special Projects and the Cellular                           Biosciences Cell Biology Program.  It is aimed at the                           development of structural information on tubulin and                            exploration of the relationship between tubulin function and                    structural features.  Particular emphasis will be placed on the                 the role of tubulin isoforms in the mechanism of function and                   control.  Monoclonal antibodies will be used to recognize                       specifc tubulins.  Separation of electrophoretically distinct                   tubulins will be investigated using immuno-affinity                             chromatography, pH gradient chromatofocusing, and affinity                      chromatography.  Photoaffinity and chemico-affinity studies will                be directed at ligand binding site localization.                                Thermodynamic and kinetic features of ligand-binding sites will                 be probed with particular emphasis on ligand-induced                            conformational changes in tubulin and proteolytic fragments.                    Protein sequencing of purified isoforms, proteolytic fragments,                 and affinity-labeled fragments will be done by mass                             spectrometric methods.  The information obtained from these                     studies will be used to develop a model of tubulin and the                      conformational effects induced by assembly and ligand binding.
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{'Name': 'University of Virginia Main Campus', 'CityName': 'CHARLOTTESVILLE', 'ZipCode': '229034833', 'PhoneNumber': '4349244270', 'StreetAddress': '1001 EMMET ST N', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Virginia', 'StateCode': 'VA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'VA05', 'ORG_UEI_NUM': 'JJG6HU8PA4S5', 'ORG_LGL_BUS_NAME': 'RECTOR & VISITORS OF THE UNIVERSITY OF VIRGINIA', 'ORG_PRNT_UEI_NUM': None}
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[{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}, {'Code': '1950', 'Text': 'METHODOLOGY'}, {'Code': '1978', 'Text': 'PROJECTS'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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