Regulation of the micF Gene and Mechanism of Action of the  Transcript as a Repressor
{'Code': '08070800', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Todd M. Martensen', 'PO_EMAI': None, 'PO_PHON': None}
Within the past few years a large number of small RNAs have                     been discovered and characterized.  Several of these have been                  found to be regulatory molecules.  One small RNA termed micF                    RNA has been hypothesized to negatively regulate the                            expressions of outer membrane protein F (ompF) at the level of                  translation in E coli.  The mechanism faction of micF RNA as a                  repressor molecular will be elucidated.  The kinetics of its                    systhesis and decay will be determined.  The in vivo levels of                  ompF m RNA in cells having micF on a multicopy plasmid, in                      reference strains and in micF deletion strain will be                           investigated.  Attempts will be made to form a micF RNA - ompF                  m RNA hybrid in vitro and its properties will be characterized.                                                                                                 The question of whether small RNA molecules complementary fo                    certain mRNA sequences can repress translation of those mRNAs                   is of much current interest.  Specifically is there a micC RNA                  that participates in the regulation of ompC synthesis?   MicF                   RNA may prove to be a prototype of other regulatory RNA genes                   that function in eubacteria and it would indeed be exciting if                  this were the case.
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[{'Code': '1154', 'Text': 'GENE EXPRESSION'}, {'Code': '1166', 'Text': 'MOLECULAR BIOCHEMISTRY'}, {'Code': '9145', 'Text': 'SPECIAL PROGRAMS-RESERVE'}]
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