New Instrumentation for Cell Cycle Research
{'Code': '08080102', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DBI', 'LongName': 'Div Of Biological Infrastructure'}}
{'SignBlockName': 'Joann P. Roskoski', 'PO_EMAI': None, 'PO_PHON': None}
The goal of the project is to develop a small, easy-to-use                      instrument to perform cell cycle studies, and related work on                   cellular proliferation, aging, partitioning and development, on                 both eukaryotes and prokaryotes in steady-state growth.  This new               technology will apply a concept currently used for cell cycle                   studies on bacteria.  In the procedure, cells are grown on a                    surface such that each time a cell divides, one new daughter cell               remains attached to the surface and the other is released form                  the surface.  As a result, newborn cells are shed continuously                  form the surface for use in any cell cycle/cell age                             investigation.  The unique aspect of the technique is that the                  investigation can be performed on large quantities of cells in                  long-term, undisturbed, balanced growth.  In the instruments to                 be developed in this project, the surfaces will  be covered with                a pattern of small spots to which cells will adhere, with a                     matrix between the spots to which the cell will not adhere or                   will adhere less firmly.  The project entails:  the production of               appropriate cell adherent surfaces the testing of the surfaces in               an experimental flow cell system, the design and construction of                the culture apparatus for large-scale production of cells of                    uniform age, and the evaluation of the instruments for cell cycle               studies on cells in steady-state growth.
{'FirstName': 'Charles', 'LastName': 'Helmstetter', 'PI_MID_INIT': 'E', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Charles E Helmstetter', 'EmailAddress': None, 'NSF_ID': '000195205', 'StartDate': '02/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Florida Institute of Technology', 'CityName': 'MELBOURNE', 'ZipCode': '329018995', 'PhoneNumber': '3216748000', 'StreetAddress': '150 W UNIVERSITY BLVD', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Florida', 'StateCode': 'FL', 'CONGRESSDISTRICT': '08', 'CONGRESS_DISTRICT_ORG': 'FL08', 'ORG_UEI_NUM': 'WNN6VH618X58', 'ORG_LGL_BUS_NAME': 'FLORIDA INSTITUTE OF TECHNOLOGY INC', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1108', 'Text': 'INSTRUMENTAT & INSTRUMENT DEVP'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8802989.xml'}