RUI: Control of Cell Proliferation and Differentiation in   Adult Cardiac Muscle
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
The aim of the proposed work is to determine the optimum                    conditions required to maintain and grow adult cardiac muscle                   cells in serum-free long term culture.  This study will formulate               an effective, serum-free, chemically defined growth medium, using               different growth factors, hormones, and chemicals for vigorous                  cell proliferation and continued survival.  The effects of growth               conditions on DNA synthesis, DNA polymerase and thymidine kinase                activities will be evaluated in these myocyte cultures.                         Biochemical and immunological studies will be done in order to                  identify and characterize myosin isoforms in these cells, and                   electron microscopy will be carried out to monitor cell fine                    structure during long term cell culture.                                             Studies of adult cardiac cell biology have been hampered by                the absence of a long term cell culture system, so that one                     immediate benefit of the work proposed will be the establishment                of such a system. The long term significance will be an increased               knowledge of the cell biology, physiology, and pharmacology of                  cardiac myocytes.
{'FirstName': 'Asish', 'LastName': 'Nag', 'PI_MID_INIT': 'C', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Asish C Nag', 'EmailAddress': None, 'NSF_ID': '000474644', 'StartDate': '08/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Oakland University', 'CityName': 'Rochester', 'ZipCode': '483094401', 'PhoneNumber': '2483704116', 'StreetAddress': '530 Wilson Hall', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Michigan', 'StateCode': 'MI', 'CONGRESSDISTRICT': '11', 'CONGRESS_DISTRICT_ORG': 'MI11', 'ORG_DUNS_NUM': '041808262', 'ORG_LGL_BUS_NAME': 'OAKLAND UNIVERSITY', 'ORG_PRNT_DUNS_NUM': '041808262'}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8709594.xml'}