An Improved Method of Microbial Cell Disruption
{'Code': '15030000', 'Directorate': {'Abbreviation': 'TIP', 'LongName': 'Dir for Tech, Innovation, & Partnerships'}, 'Division': {'Abbreviation': 'TI', 'LongName': 'Translational Impacts'}}
{'SignBlockName': 'Ritchie B. Coryell', 'PO_EMAI': None, 'PO_PHON': None}
Narrative: Disruption of the cell envelope is one of the first                  steps in the recovery of materials contained within microbial                   cells.  Conventional techniques typically involve high shear,                   heat generation, and multiple stage processing, all factors which               can lead to degradation of the desired product.  Furthermore,                   these techniques often lead to the creation of small cell wall                  fragments which adds to the burden of downstream purification.                  The present proposal suggests a method of cell disruption which                 may avoid these difficulties, improve product yields, and prove                 more economical as well.  The method, a modified form of                        explosive decompression, is based on the large specific volume                  changes which may be rapidly effected upon depressurization of a                supercritical fluid or low-boiling liquid.  Any process requiring               microbial cell disruption may benefit significantly from the                    proposed explosive disruption method.
{'FirstName': 'Trevor', 'LastName': 'Castor', 'PI_MID_INIT': 'P', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Trevor P Castor', 'EmailAddress': 'aphios@aol.com', 'NSF_ID': '000159019', 'StartDate': '02/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Aphios Corporation', 'CityName': 'WOBURN', 'ZipCode': '018016307', 'PhoneNumber': '6179326933', 'StreetAddress': '25 OLYMPIA AVE STE K-500', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Massachusetts', 'StateCode': 'MA', 'CONGRESSDISTRICT': '05', 'CONGRESS_DISTRICT_ORG': 'MA05', 'ORG_UEI_NUM': 'HDE2J1H521A7', 'ORG_LGL_BUS_NAME': 'APHIOS CORP', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
{'Code': '5371', 'Text': 'SBIR Phase I'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8760517.xml'}