Molecular Analysis of the TMV-Movement Protein, A Virulence Factor
{'Code': '08070100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Philip Harriman', 'PO_EMAI': None, 'PO_PHON': None}
Plant viruses move throughout their hosts by unknown mechanisms                 that involve expression of one or more viral gene products.                     Since viruses that do not spread in their hosts are generally not               pathogenic, the capacity for movement in the inoculated plant                   constitutes a virulence function.  However, the nature of the                   protein(s) and the molecular and cellular mechanisms by which the               proteins function are not yet determined.  Recently this                        laboratory demonstrated that the 30 kDa protein of Tobacco Mosaic               Virus (TMV) is responsible for potentiating cell-to-cell movement               of TMV by using transgenic plants that constitutively express a                 chimeric gene encoding the 30 kDa protein.  This excellent system               will be used to:  1) purify and characterize the TMV-30 kDa                     protein (referred to here as the movement protein, MP);  2) to                  generate polyclonal antibodies against the TMV-MP;  3) to                       localize the MP in transgenic and TMV-infected tobacco plants;                  and 4) to genetically map the functional domains/amino acids of                 the MP by site directed and random mutagenesis of an infectious                 cloned cDNA of TMV, rescuing defective mutants on transgenic                    plants that express the MP.  By characterizing the virus mutants                that are defective in movement, as determined by ability to move                in the transgenic plants that express the 30 kDa protein, the                   results of this research will lead to an understanding of the                   function of TMV-30 kDa protein in potentiating the movement of                  TMV in tobacco plants.
{'FirstName': 'Roger', 'LastName': 'Beachy', 'PI_MID_INIT': 'N', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Roger N Beachy', 'EmailAddress': 'RnBeachy@danforthcenter.org', 'NSF_ID': '000290415', 'StartDate': '04/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Washington University', 'CityName': 'SAINT LOUIS', 'ZipCode': '63110', 'PhoneNumber': '3147474134', 'StreetAddress': 'ONE BROOKINGS DR', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Missouri', 'StateCode': 'MO', 'CONGRESSDISTRICT': '01', 'CONGRESS_DISTRICT_ORG': 'MO01', 'ORG_UEI_NUM': 'L6NFUM28LQM5', 'ORG_LGL_BUS_NAME': 'WASHINGTON UNIVERSITY, THE', 'ORG_PRNT_UEI_NUM': None}
{'Name': None, 'CityName': None, 'StateCode': None, 'ZipCode': None, 'StreetAddress': None, 'CountryCode': None, 'CountryName': None, 'StateName': None, 'CountryFlag': None, 'CONGRESSDISTRICT': None, 'CONGRESS_DISTRICT_PERF': '""'}
[{'Code': '1112', 'Text': 'Genetic Mechanisms'}, {'Code': '1116', 'Text': 'EUKARYOTIC GENETICS'}]
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8717012.xml'}