Flagellar Ultrastructure and Phylogeny of Golden Algae
{'Code': '08010207', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'DEB', 'LongName': 'Division Of Environmental Biology'}}
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The structure of the flagella (swimming organelles) and                 associated subcellular components will be examined in golden                    algae by Dr. Robert Andersen of DePaul University.  These algae                 are photosynthetic organisms, but they have cell cytoskeleton                   features similar to animals.  The combination of photosynthetic                 and cytoskeletal features with the small size and rapid growth of               the organisms makes the golden algae a good experimental model                  system where the results can aid in our understanding plants and                animals.  Two techniques, electron microscopy and monoclonal                    antibody immunofluorescent studies, will be employed.                                   Microtubules, which are major components of flagella and                cell cytoskeletons, will be identified using a fluorescent stain                conjugated to tubulin protein molecules.  The position of                       microtubules in "ordinary" cells will be compared to the position               of microtubules in dividing cells.  Furthermore, as one cell                    divides into two cells, the developmental process of producing                  two flagellar apparatuses and two cytoskeletons will be                         investigated.  Electron microscopy will be employed to provide                  fine details of these structures and processes.  The findings                   should help us better understand the structure and change in                    structure of flagellar apparatuses and cytoskeletons in golden                  algae.  In addition, the findings should also help us better                    understand evolutionary relationships among these and other                     algae.
{'FirstName': 'Robert', 'LastName': 'Andersen', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Robert A Andersen', 'EmailAddress': 'raa48@uw.edu', 'NSF_ID': '000400729', 'StartDate': '10/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'DePaul University', 'CityName': 'CHICAGO', 'ZipCode': '606042287', 'PhoneNumber': '3123627388', 'StreetAddress': '1 E JACKSON BLVD', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Illinois', 'StateCode': 'IL', 'CONGRESSDISTRICT': '07', 'CONGRESS_DISTRICT_ORG': 'IL07', 'ORG_UEI_NUM': 'MNZ8KMRWTDB6', 'ORG_LGL_BUS_NAME': 'DEPAUL UNIVERSITY', 'ORG_PRNT_UEI_NUM': None}
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{'Code': '112700', 'Text': 'Evolutionary Processes'}
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