Biochemistry of Dyneins
{'Code': '08070600', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'MCB', 'LongName': 'Div Of Molecular and Cellular Bioscience'}}
{'SignBlockName': 'Maryanna P. Henkart', 'PO_EMAI': None, 'PO_PHON': None}
The determination of the structure of the dyneins is of                    fundamental importance to the understanding of microtubule-                     mediated motility.  This project will identify the structural                   relationships among the dyneins which are expressed in the                      gametes and early embryo of a single animal species, the sea                    urchin, a uniquely well-suited model system.  By focussing on a                 single animal which expresses multiple dyneins in different                     developmental contexts, information related directly to function                and to development will be obtained.                                                                                                                            Specific aims are to: determine the polypeptide composition of                  the ca. 21S ciliary dynein which is obtained by the high salt                   extraction of embryonic cilia; raise new antibodies to ciliary                  dynein polypeptides; and to determine the structure of the                      ciliary 12S and 21S dyneins by electron microscopy.  The results                of these studies will add to our understanding of dynein                        structual heterogeneity and how it might affect dynein function.
{'FirstName': 'David', 'LastName': 'Asai', 'PI_MID_INIT': 'J', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'David J Asai', 'EmailAddress': 'david_asai@hmc.edu', 'NSF_ID': '000473906', 'StartDate': '06/01/1989', 'EndDate': None, 'RoleCode': 'Principal Investigator'}
{'Name': 'Purdue Research Foundation', 'CityName': 'WEST LAFAYETTE', 'ZipCode': '479064353', 'PhoneNumber': '3174946200', 'StreetAddress': '1281 WIN HENTSCHEL BLVD STE 2500', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'Indiana', 'StateCode': 'IN', 'CONGRESSDISTRICT': '04', 'CONGRESS_DISTRICT_ORG': 'IN04', 'ORG_UEI_NUM': 'C1G1LGYJF5G5', 'ORG_LGL_BUS_NAME': 'PURDUE RESEARCH FOUNDATION', 'ORG_PRNT_UEI_NUM': 'YRXVL4JYCEF5'}
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{'Code': '1114', 'Text': 'Cellular Dynamics and Function'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1988&All=true', 'xml': '8818762.xml'}