33K/BETA-Gamma Transducin Complex of Mammalian Rod          Photoreceptors
{'Code': '08090200', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'name not available', 'PO_EMAI': None, 'PO_PHON': None}
This research project aims to characterize further the                     mechanisms which regulate the phosphorylation in darkness and the               light-activated dephosphorylation of the visual cell specific,                  33K/beta-gamma transducin (33K/TBY) complex.  The 33K (33                       Kilodalton) protein which is phosphorylated by cyclic nucleotide-               dependent protein kinase, in vitro, has been purified to near                   homogeneity and shown to form a complex with the beta-gamma                     subunits of transducin.  In situ, the level of phosphorylated 33K               in dark-adapted ROS is enhanced further by incubating retinas                   with drugs which increase the concentration of cyclic GMP.                      Immunocytochemistry using monospecific antibodies against bovine                33K or the beta/gamma subunits of transducin shows that both 33K                and T-beta/gamma are localized in rod outer segments as well as                 the inner segments of bovine and mouse photoreceptors.                          Biochemical fractionation shows further that the 33K protein is                 bound firmly to ROS membranes and that the 33K is probably                      phosphorylated whereas the soluble 33K/TBY complex is                           unphosphorylated.  These findings suggest that the binding of 33K               and/or the 33K/TBY complex to ROS membranes is controlled by the                phosphorylation of 33K.  The P.I.s will evaluate this hypothesis                and find the role of 33K/TBY complex in a series of related                     projects that (1) characterize the membrane-bound species of 33K                (m33K); (2) evaluate the partitioning of 33K between the soluble                and membrane fractions of ROS; (3) investigate the light-                       initiated dephosphorylation of m33K; (4) identify and                           characterize the protein kinase which phosphorylates m33K; and                  (5) determine whether 33K and/or the 33K/TBY complex interacts                  with transducin and/or modulates the participation of transducin                in the PDE-activation cascade.  This systematic study of the                    33K/TBY complex will provide a fuller understanding of light-                   activated events in rod visual cells and project a role for the                 33K/TBY complex in ROS function.
[{'FirstName': 'Rehwa', 'LastName': 'Lee', 'PI_MID_INIT': 'H', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Rehwa H Lee', 'EmailAddress': None, 'NSF_ID': '000107693', 'StartDate': '01/01/1988', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}, {'FirstName': 'Richard', 'LastName': 'Lolley', 'PI_MID_INIT': 'N', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Richard N Lolley', 'EmailAddress': None, 'NSF_ID': '000406299', 'StartDate': '01/01/1988', 'EndDate': None, 'RoleCode': 'Principal Investigator'}]
{'Name': 'University of California-Los Angeles', 'CityName': 'LOS ANGELES', 'ZipCode': '900244200', 'PhoneNumber': '3107940102', 'StreetAddress': '10889 WILSHIRE BLVD STE 700', 'StreetAddress2': None, 'CountryName': 'United States', 'StateName': 'California', 'StateCode': 'CA', 'CONGRESSDISTRICT': '36', 'CONGRESS_DISTRICT_ORG': 'CA36', 'ORG_UEI_NUM': 'RN64EPNH8JC6', 'ORG_LGL_BUS_NAME': 'UNIVERSITY OF CALIFORNIA, LOS ANGELES', 'ORG_PRNT_UEI_NUM': None}
{'Name': 'University of California LA Ctr for Health Sci Sch of Medicine', 'CityName': 'Los Angeles', 'StateCode': 'CA', 'ZipCode': '900241301', 'StreetAddress': '405 Hilgard Ave', 'CountryCode': 'US', 'CountryName': 'United States', 'StateName': 'California', 'CountryFlag': '1', 'CONGRESSDISTRICT': '36', 'CONGRESS_DISTRICT_PERF': 'CA36'}
{'Code': '1192', 'Text': 'NEURONAL AND GLIAL MECHANISMS'}
{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8708416.xml'}