Mechanism of Action of Lipoxygenase
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Lipoxygenases are iron-containing dioxygenases the catalyze                     the hydopeoxidation of polyunsaturated lipids.  This                            laboratory has obtained the first primary sequence of                           lipoxygenase, the L-1 isozyme of soybean.  It contains a 40                     amino acid sequence region which might contain the Fe-                          octahedral binding site whose presence in the protein is                        supported by physical evidene.  The sequence is highly                          conserved in the other two major isozymes of soybeans.  He                      now proposes to clone and express the enzyme in E.coli in                       high amounts, to determine its three dimensional structure by                   x-ray crystallography and to perform site-directed                              mutagenesis of selected possibly key amino-acids in the                         enzyme's sequence.                                                                                                                                              This research will contribute to the ultimate understanding                     of structure function relationship of the lipoxygenases and                     to a better preception of the role of the various ligands in                    non-heme non-sulfur iron proteins.  These are expecially                        interesting enzymes because while catalyzing the same primary                   reaction, they exhibit a great diversity in their enzymic                       behavior.  Thus a wide range of behavior remains to be                          explained by rather limited variations in structure.  Dr.                       Axelrod has had a long and distringuished career in                             enzymology and has been responsible for much of our                             fundamental knowledge about plant lipoxygenases.
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{'Code': '1144', 'Text': 'Molecular Biophysics'}
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