Analysis of Blastoderm-Specific Genes in Drosophila
{'Code': '08090100', 'Directorate': {'Abbreviation': 'BIO', 'LongName': 'Direct For Biological Sciences'}, 'Division': {'Abbreviation': 'IOS', 'LongName': 'Division Of Integrative Organismal Systems'}}
{'SignBlockName': 'Judith Plesset', 'PO_EMAI': None, 'PO_PHON': None}
The goal of this proposal is to understand the function of            the >Drosophila> segmentation gene >tailless> (>tll>).  The mutant              phenotype consists of characteristic defects or absence of                      structures of segmental origin located near the anterior and the                posterior of the ectodermal region of the blastoderm.  The                      remaining segments appear larger.  Like the identified "gap"                    segmentation genes, >tll> mutants alter the >ftz> blastoderm striping           pattern.  Hence, >tll> acts early in development.  However, it                  appears to serve a unique function, as there are no other                       zygotically active genes altering both poles of the embryo.                     While the development of a segment pattern in insects has been                  explained on the basis of a double gradient resulting from the                  action of two separate systems, it has not been predicted that                  the products of a single gene might be involved in establishing                 the two opposite poles.  The objectives are to use molecular                    techniques to isolate the >tll> gene and to characterize its gene               product.  In situ hybridizations will be carried out to early                   embryos to determine whether the transcript is localized to the                 anterior and posterior regions altered in the mutants.  The                     transcription unit will be defined and the transcription pattern                determined.  The gene will be sequenced, antibodies will be                     raised against fusion proteins, and the effect of mutations in                  other segmentation genes on expression of >tll> will be assessed.                    The experiments will provide insight into a unique gene                    which controls segmentation at the two ends of an organism, and                 so into the process of cell determination at the onset of                       development.
[{'FirstName': 'Judith', 'LastName': 'Lengyel', 'PI_MID_INIT': 'A', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'Judith A Lengyel', 'EmailAddress': None, 'NSF_ID': '000445438', 'StartDate': '07/15/1987', 'EndDate': None, 'RoleCode': 'Principal Investigator'}, {'FirstName': 'John', 'LastName': 'Merriam', 'PI_MID_INIT': 'R', 'PI_SUFX_NAME': None, 'PI_FULL_NAME': 'John R Merriam', 'EmailAddress': 'jmerriam@mcdb.ucla.edu', 'NSF_ID': '000340614', 'StartDate': '07/15/1987', 'EndDate': None, 'RoleCode': 'Co-Principal Investigator'}]
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{'url': 'https://www.nsf.gov/awardsearch/download?DownloadFileName=1987&All=true', 'xml': '8710451.xml'}