Another RESOLFT-based technique proposed early on was Ground State Depletion microscopy, which uses an excitation pulse to boost off-axis ground-state electrons to a long-lived higher energy state before exciting remaining ground-state electrons with a fluorescence excitation pulse.
Another RESOLFT-based technique proposed early on was Ground State Depletion microscopy-axis ground-state electrons to a long-lived higher energy state before exciting remaining ground-state electrons with a fluorescence excitation pulse. Ground State Depletion microscopy uses an excitation pulse to boost off.