Samples are transferred through baths of progressively more concentrated ethanol to remove the water, followed by a hydrophobic clearing agent (such as xylene) to remove the alcohol, and finally molten paraffin wax, the infiltration agent, which replaces the xylene.
Samples are transferred through baths of progressively more concentrated ethanol. This was to remove the water. The water is the infiltration agent. Samples are followed by a hydrophobic clearing agent (such as xylene). This was to remove the alcohol, and finally molten paraffin wax. Molten paraffin wax is the infiltration agent. The water is followed by a hydrophobic clearing agent (such as xylene). This was to remove the alcohol, and finally molten paraffin wax. The water is the infiltration agent. The water replaces the xylene. The water is the infiltration agent.