In contrast to Polymerase Cycling Assembly, Gibson Assembly is a single-step, isothermal reaction with larger sequence-length capacity; ergo, it is used in place of Polymerase Cycling Assembly for genomes larger than 6 kb.
Gibson Assembly is a single-step, isothermal reaction with larger sequence-length capacity. This is in contrast to Polymerase Cycling Assembly. It is used in place of Polymerase Cycling Assembly for genomes larger than 6 kb. This was ergo.