Although this method is widely used for immobilization of proteins onto agarose resin, there are drawbacks to the technique: the covalent bond is relatively unstable at pH extremes and there is a consistent leakage of the immobilized ligand from the resin during use.
This method is widely used for immobilization of proteins onto agarose resin. There are drawbacks to the technique. The covalent bond is relatively unstable at pH extremes. There is a consistent leakage of the immobilized. This immobilized is ligand from the resin during use.