The tagging portion of the Pitcher is designed not to hybridize to the target sequence, during the DPOTM primer extension, the Pitcher is cleaved by the exonuclease activity of DNA polymerase, and then the tagging portion is released.
The tagging portion of the Pitcher is designed not to hybridize to the target sequence. The Pitcher is cleaved by the exonuclease activity of DNA polymerase. This was during the DPOTM primer extension. The tagging portion is released.