For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, different final concentration of IPTG may also be used.
For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, different final concentration of IPTG may also be used. This was however.