In the late seventies and early eighties, Jo Messing and his colleagues developed the, shotgun sequencing and site-directed mutagenesis became widely used to sequence large DNA molecules like human chromosomes and to engineer genes and proteins.
Jo Messing developed the, shotgun sequencing and site. His colleagues developed the, shotgun sequencing and site. Directed mutagenesis became widely used to sequence large DNA molecules like human chromosomes and to engineer genes and proteins. This was in the late seventies. This was in early eighties.